LOCUS       sequence-495978-       12603 bp    DNA     circular SYN 23-DEC-2025
DEFINITION  This plasmid expresses the BRCA1 sequence with Y1845D mutation, the 
            construct includes a HBBIVS2 intron sequence at the 3' end of the 
            mRuby sequence to improve the stable integration into cell lines.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12603)
  AUTHORS   Gracia B, Zhang XH, Montes P, Pham TC, Huang M, Chen J, Karras GI
  TITLE     HSP90 buffers deleterious genetic variations in BRCA1.
  JOURNAL   Mol Cell. 2025 Nov 19:S1097-2765(25)00867-6. doi: 
            10.1016/j.molcel.2025.10.026.
  PUBMED    41265458
REFERENCE   2  (bases 1 to 12603)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 23, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Cell. 
            2025 Nov 19:S1097-2765(25)00867-6. doi: 
            10.1016/j.molcel.2025.10.026."
COMMENT     Sequence Label: 
            pKlab1070-HBEGF-2a-3xFLAG-BRCA1(full-length)-Y1845D-2a-mRuby3-HBBIVS
            2-WPRE
FEATURES             Location/Qualifiers
     source          1..12603
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(61..78)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(171..759)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(251..270)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(930..1721,1722..1790))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     1553..1572
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(1791..1895)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     2196..2218
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     2210..2227
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     2211..2227
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     2261..2280
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        2261..2279
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             3371..3424
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             3443..3508
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     protein_bind    3533..3557
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             9155..9211
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             9230..9937
                     /codon_start=1
                     /product="monomeric red fluorescent protein derived from 
                     mRuby2, with improved brightness and photostability (Bajar 
                     et al., 2016)"
                     /label=mRuby3
                     /translation="VSKGEELIKENMRMKVVMEGSVNGHQFKCTGEGEGRPYEGVQTMR
                     IKVIEGGPLPFAFDILATSFMYGSRTFIKYPADIPDFFKQSFPEGFTWERVTRYEDGGV
                     VTVTQDTSLEDGELVYNVKVRGVNFPSNGPVMQKKTKGWEPNTEMMYPADGGLRGYTDI
                     ALKVDGGGHLHCNFVTTYRSKKTVGNIKMPGVHAVDHRLERIEESDNETYVVQREVAVA
                     KYSNLGGGMDELYK"
     misc_feature    10903..11491
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(10956..10976)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(11374..11385)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(11529..11546)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    11535..11759
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
ORIGIN
        1 gtgccagctg cattaacatg gtcatagctg tttccttgcg tattgggcgc tctccgcttc
       61 ctcgctcact gactcgctgc gctcggtcgt tcgggtaaag cctggggtgc ctaatgagca
      121 aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt tttccatagg
      181 ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg
      241 acaggactat aaagatacca ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt
      301 ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc cttcgggaag cgtggcgctt
      361 tctcatagct cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc caagctgggc
      421 tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt
      481 gagtccaacc cggtaagaca cgacttatcg ccactggcag cagccactgg taacaggatt
      541 agcagagcga ggtatgtagg cggtgctaca gagttcttga agtggtggcc taactacggc
      601 tacactagaa gaacagtatt tggtatctgc gctctgctga agccagttac cttcggaaaa
      661 agagttggta gctcttgatc cggcaaacaa accaccgctg gtagcggtgg tttttttgtt
      721 tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag aagatccttt gatcttttct
      781 acggggtctg acgctcagtg gaacgaaaac tcacgttaag ggattttggt catgagatta
      841 tcaaaaagga tcttcaccta gatcctttta aattaaaaat gaagttttaa atcaatctaa
      901 agtatatatg agtaaacttg gtctgacagt taccaatgct taatcagtga ggcacctatc
      961 tcagcgatct gtctatttcg ttcatccata gttgcctgac tccccgtcgt gtagataact
     1021 acgatacggg agggcttacc atctggcccc agtgctgcaa tgataccgcg agaaccacgc
     1081 tcaccggctc cagatttatc agcaataaac cagccagccg gaagggccga gcgcagaagt
     1141 ggtcctgcaa ctttatccgc ctccatccag tctattaatt gttgccggga agctagagta
     1201 agtagttcgc cagttaatag tttgcgcaac gttgttgcca ttgctacagg catcgtggtg
     1261 tcacgctcgt cgtttggtat ggcttcattc agctccggtt cccaacgatc aaggcgagtt
     1321 acatgatccc ccatgttgtg caaaaaagcg gttagctcct tcggtcctcc gatcgttgtc
     1381 agaagtaagt tggccgcagt gttatcactc atggttatgg cagcactgca taattctctt
     1441 actgtcatgc catccgtaag atgcttttct gtgactggtg agtactcaac caagtcattc
     1501 tgagaatagt gtatgcggcg accgagttgc tcttgcccgg cgtcaatacg ggataatacc
     1561 gcgccacata gcagaacttt aaaagtgctc atcattggaa aacgttcttc ggggcgaaaa
     1621 ctctcaagga tcttaccgct gttgagatcc agttcgatgt aacccactcg tgcacccaac
     1681 tgatcttcag catcttttac tttcaccagc gtttctgggt gagcaaaaac aggaaggcaa
     1741 aatgccgcaa aaaagggaat aagggcgaca cggaaatgtt gaatactcat actcttcctt
     1801 tttcaatatt attgaagcat ttatcagggt tattgtctca tgagcggata catatttgaa
     1861 tgtatttaga aaaataaaca aataggggtt ccgcgcacat ttccccgaaa agtgccacct
     1921 aaattgtaag cgttaatatt ttgttaaaat tcgcgttaaa tttttgttaa atcagctcat
     1981 tttttaacca ataggccgaa atcggcaaaa tcccttataa atcaaaagaa tagaccgaga
     2041 tagggttgag tggccgctac agggcgctcc cattcgccat tcaggctgcg caactgttgg
     2101 gaagggcgtt tcggtgcggg cctcttcgct attacgccag ctggcgaaag ggggatgtgc
     2161 tgcaaggcga ttaagttggg taacgccagg gttttcccag tcacgacgtt gtaaaacgac
     2221 ggccagtccc ccggtcaggc aacatcaccc gagcgcgacg taatacgact cactataggg
     2281 cgaattggcg gaaggccgtc aaggccgcat acgcgtccat gtcttcggaa atacaaggac
     2341 ttctgcatcc atggagaatg caaatatgtg aaggagctcc gggctccctc ctgcatgtaa
     2401 gtgccccttc cccagggctg aatctcatca gcacactttg tcagccacgt ggctgttcct
     2461 cgttgtcact gttccttgaa ttcatgattt cacccagttt cttctcaacc tctgggcgga
     2521 agttgggagg aggggaaata tatttttagt cagcggaagc cccctccccc ctataggatg
     2581 caatttcctg tggtatggtt ttgtgacgtg ctttaatcct tggggacatt tcctgcttgc
     2641 ccagaaatga gcatgtggct aggacagctg gcacctgaag gcaggccctt aattcttgcc
     2701 tgatgcccta ctctgggagg gagaagccag taggaaacat ggcagagtgg gcttccaggg
     2761 cagagtagag ctcctgtggg aaggtaggaa gtgcatttgg atgcatgatg tataggtatg
     2821 tgtgtatttg ggtttatgtg catgtaagtg tgcaaatgtg gattgactgt gaggcatggc
     2881 aggactgtac agagagggat catcatggcg gcaggttgag gcctctcttt cttcttcctt
     2941 atcccagcaa ggacgaggag gtgggagaca tggagagtac tggcctttgg ccacgttgtg
     3001 agagaacaat tcctttgtgc agggttcaca ggaaatggaa cctgacccat taggcatcag
     3061 cccccggaat tctagggcgc agtagtccag ggtttccttg atgatgtcat acttatcctg
     3121 tccctttttt ttccacagct gccacccggg ttaccatgga aaaaggtgtc atgggctgag
     3181 cctcccagtg gaaaatcgct tatataccta tgaccacaca accatcctgg ccgtggtggc
     3241 tgtggtgctg tcatctgtct gtctgctggt catcgtgggg cttctcatgt ttaggtacca
     3301 taggagagga ggttatgatg tggaaaatga agagaaagtg aagttgggca tgactaattc
     3361 ccacggatcc gagggcagag gaagtctgct aacatgcggt gacgtcgagg agaatcctgg
     3421 cccagcaccg ggatccaccg gtgactacaa agaccatgac ggtgattata aagatcatga
     3481 catcgactac aaggatgacg atgacaagcc agtgtggtgg aattcgatat caacaagttt
     3541 gtacaaaaaa gttggcgatt tatctgctct tcgcgttgaa gaagtacaaa atgtcattaa
     3601 tgctatgcag aaaatcttag agtgtcccat ctgtctggag ttgatcaagg aaccggtgtc
     3661 cacaaagtgt gaccacatat tttgcaaatt ttgcatgctg aaacttctca accagaagaa
     3721 agggccttca cagtgtcctt tatgtaagaa tgatataacc aaaaggagcc tacaagaaag
     3781 tacgagattt agtcaacttg ttgaagagct attgaaaatc atttgtgctt ttcagcttga
     3841 cacaggtttg gagtatgcaa acagctataa ttttgcaaaa aaggaaaata actctcctga
     3901 acatctaaaa gatgaagttt ctatcatcca aagtatgggc tacagaaacc gtgccaaaag
     3961 acttctacag agtgaacccg aaaatccttc cttgcaggaa accagtctca gtgtccaact
     4021 ctctaacctt ggaactgtga gaactctgag gacaaagcag cggatacaac ctcaaaagac
     4081 gtctgtctac attgaattgg gatctgattc ttctgaagat accgttaata aggcaactta
     4141 ttgcagtgtg ggagatcaag aattgttaca aatcacccct caaggaacca gggatgaaat
     4201 cagtttggat tctgcaaaaa aggctgcttg tgaattttct gagacggatg taacaaatac
     4261 tgaacatcat caacccagta ataatgattt gaacaccact gagaagcgtg cagctgagag
     4321 gcatccagaa aagtatcagg gtagttctgt ttcaaacttg catgtggagc catgtggcac
     4381 aaatactcat gccagctcat tacagcatga gaacagcagt ttattactca ctaaagacag
     4441 aatgaatgta gaaaaggctg aattctgtaa taaaagcaaa cagcctggct tagcaaggag
     4501 ccaacataac agatgggctg gaagtaagga aacatgtaat gataggcgga ctcccagcac
     4561 agaaaaaaag gtagatctga atgctgatcc cctgtgtgag agaaaagaat ggaataagca
     4621 gaaactgcca tgctcagaga atcctagaga tactgaagat gttccttgga taacactaaa
     4681 tagcagcatt cagaaagtta atgagtggtt ttccagaagt gatgaactgt taggttctga
     4741 tgactcacat gatggggagt ctgaatcaaa tgccaaagta gctgatgtat tggacgttct
     4801 aaatgaggta gatgaatatt ctggttcttc agagaaaata gacttactgg ccagtgatcc
     4861 tcatgaggct ttaatatgta aaagtgaaag agttcactcc aaatcagtag agagtaatat
     4921 tgaagacaaa atatttggga aaacctatcg gaagaaggca agcctcccca acttaagcca
     4981 tgtaactgaa aatctaatta taggagcatt tgttactgag ccacagataa tacaagagcg
     5041 tcccctcaca aataaattaa agcgtaaaag gagacctaca tcaggccttc atcctgagga
     5101 ttttatcaag aaagcagatt tggcagttca aaagactcct gaaatgataa atcagggaac
     5161 taaccaaacg gagcagaatg gtcaagtgat gaatattact aatagtggtc atgagaataa
     5221 aacaaaaggt gattctattc agaatgagaa aaatcctaac ccaatagaat cactcgaaaa
     5281 agaatctgct ttcaaaacga aagctgaacc tataagcagc agtataagca atatggaact
     5341 cgaattaaat atccacaatt caaaagcacc taaaaagaat aggctgagga ggaagtcttc
     5401 taccaggcat attcatgcgc ttgaactagt agtcagtaga aatctaagcc cacctaattg
     5461 tactgaattg caaattgata gttgttctag cagtgaagag ataaagaaaa aaaagtacaa
     5521 ccaaatgcca gtcaggcaca gcagaaacct acaactcatg gaaggtaaag aacctgcaac
     5581 tggagccaag aagagtaaca agccaaatga acagacaagt aaaagacatg acagtgatac
     5641 tttcccagag ctgaagttaa caaatgcacc tggttctttt actaagtgtt caaataccag
     5701 tgaacttaaa gaatttgtca atcctagcct tccaagagaa gaaaaagaag agaaactaga
     5761 aacagttaaa gtgtctaata atgctgaaga ccccaaagat ctcatgttaa gtggagaaag
     5821 ggttttgcaa actgaaagat ctgtagagag tagcagtatt tcactggtac ctggtactga
     5881 ttatggcact caggaaagta tctcgttact ggaagttagc actctaggga aggcaaaaac
     5941 agaaccaaat aaatgtgtga gtcagtgtgc agcatttgaa aaccccaagg gactaattca
     6001 tggttgttcc aaagataata gaaatgacac agaaggcttt aagtatccat tgggacatga
     6061 agttaaccac agtcgggaaa caagcataga aatggaagaa agtgaacttg atgctcagta
     6121 tttgcagaat acattcaagg tttcaaagcg ccagtcattt gctctgtttt caaatccagg
     6181 aaatgcagaa gaggaatgtg caacattctc tgcccactct gggtccttaa agaaacaaag
     6241 tccaaaagtc acttttgaat gtgaacaaaa ggaagaaaat caaggaaaga atgagtctaa
     6301 tatcaagcct gtacagacag ttaatatcac tgcaggcttt cctgtggttg gtcagaaaga
     6361 taagccagtt gataatgcca aatgtagtat caaaggaggc tctaggtttt gtctatcatc
     6421 tcagttcaga ggcaacgaaa ctggactcat tactccaaat aaacatggac ttttacaaaa
     6481 cccatatcgt ataccaccac tttttcccat caagtcattt gttaaaacta aatgtaagaa
     6541 aaatctgcta gaggaaaact ttgaggaaca ttcaatgtca cctgaaagag aaatgggaaa
     6601 tgagaacatt ccaagtacag tgagcacaat tagccgtaat aacattagag aaaatgtttt
     6661 taaaggagcc agctcaagca atattaatga agtaggttcc agtactaatg aagtgggctc
     6721 cagtattaat gaaataggtt ccagtgatga aaacattcaa gcagaactag gtagaaacag
     6781 agggccaaaa ttgaatgcta tgcttagatt aggggttttg caacctgagg tctataaaca
     6841 aagtcttcct ggaagtaatt gtaagcatcc tgaaataaaa aagcaagaat atgaagaagt
     6901 agttcagact gttaatacag atttctctcc atatctgatt tcagataact tagaacagcc
     6961 tatgggaagt agtcatgcat ctcaggtttg ttctgagaca cctgatgacc tgttagatga
     7021 tggtgaaata aaggaagata ctagttttgc tgaaaatgac attaaggaaa gttctgctgt
     7081 ttttagcaaa agcgtccaga gaggagagct tagcaggagt cctagccctt tcacccatac
     7141 acatttggct cagggttacc gaagaggggc caagaaatta gagtcctcag aagagaactt
     7201 atctagtgag gatgaagagc ttccctgctt ccaacacttg ttatttggta aagtaaacaa
     7261 tataccttct cagtctacta ggcatagcac cgttgctacc gagtgtctgt ctaagaacac
     7321 agaggagaat ttattatcat tgaagaatag cttaaatgac tgcagtaacc aggtaatatt
     7381 ggcaaaggca tctcaggaac atcaccttag tgaggaaaca aaatgttctg ctagcttgtt
     7441 ttcttcacag tgcagtgaat tggaagactt gactgcaaat acaaacaccc aggatccttt
     7501 cttgattggt tcttccaaac aaatgaggca tcagtctgaa agccagggag ttggtctgag
     7561 tgacaaggaa ttggtttcag atgatgaaga aagaggaacg ggcttggaag aaaataatca
     7621 agaagagcaa agcatggatt caaacttagg tgaagcagca tctgggtgtg agagtgaaac
     7681 aagcgtctct gaagactgct cagggctatc ctctcagagt gacattttaa ccactcagca
     7741 gagggatacc atgcaacata acctgataaa gctccagcag gaaatggctg aactagaagc
     7801 tgtgttagaa cagcatggga gccagccttc taacagctac ccttccatca taagtgactc
     7861 ctctgccctt gaggacctgc gaaatccaga acaaagcaca tcagaaaaag cagtattaac
     7921 ttcacagaaa agtagtgaat accctataag ccagaatcca gaaggccttt ctgctgacaa
     7981 gtttgaggtg tctgcagata gttctaccag taaaaataaa gaaccaggag tggaaaggtc
     8041 atccccttct aaatgcccat cattagatga taggtggtac atgcacagtt gctctgggag
     8101 tcttcagaat agaaactacc catctcaaga ggagctcatt aaggttgttg atgtggagga
     8161 gcaacagctg gaagagtctg ggccacacga tttgacggaa acatcttact tgccaaggca
     8221 agatctagag ggaacccctt acctggaatc tggaatcagc ctcttctctg atgaccctga
     8281 atctgatcct tctgaagaca gagccccaga gtcagctcgt gttggcaaca taccatcttc
     8341 aacctctgca ttgaaagttc cccaattgaa agttgcagaa tctgcccagg gtccagctgc
     8401 tgctcatact actgatactg ctgggtataa tgcaatggaa gaaagtgtga gcagggagaa
     8461 gccagaattg acagcttcaa cagaaagggt caacaaaaga atgtccatgg tggtgtctgg
     8521 cctgacccca gaagaattta tgctcgtgta caagtttgcc agaaaacacc acatcacttt
     8581 aactaatcta attactgaag agactactca tgttgttatg aaaacagatg ctgagtttgt
     8641 gtgtgaacgg acactgaaat attttctagg aattgcggga ggaaaatggg tagttagcta
     8701 tttctgggtg acccagtcta ttaaagaaag aaaaatgctg aatgagcatg attttgaagt
     8761 cagaggagat gtggtcaatg gaagaaacca ccaaggtcca aagcgagcaa gagaatccca
     8821 ggacagaaag atcttcaggg ggctagaaat ctgttgctat gggcccttca ccaacatgcc
     8881 cacagatcaa ctggaatgga tggtacagct gtgtggtgct tctgtggtga aggagctttc
     8941 atcattcacc cttggcacag gtgtccaccc aattgtggtt gtgcagccag atgcctggac
     9001 agaggacaat ggcttccatg caattgggca gatgtgtgag gcacctgtgg tgacccgaga
     9061 gtgggtgttg gacagtgtag cactcgatca gtgccaggag ctggacacct acctgatacc
     9121 ccagatcccc cacagccact acaccggtgg ctccgccacc aatttcagcc tgctcaagca
     9181 ggctggcgac gtggaggaaa acccaggacc tgcgccgggt tcgcaattgg tgtctaaggg
     9241 cgaagagctg atcaaggaaa atatgcgtat gaaggtggtc atggaaggtt cggtcaacgg
     9301 ccaccaattc aaatgcacag gtgaaggaga aggcagaccg tacgagggag tgcaaaccat
     9361 gaggatcaaa gtcatcgagg gaggacccct gccatttgcc tttgacattc ttgccacgtc
     9421 gttcatgtat ggcagccgta cctttatcaa gtacccggcc gacatccctg atttctttaa
     9481 acagtccttt cctgagggtt ttacttggga aagagttacg agatacgaag atggtggagt
     9541 cgtcaccgtc acgcaggaca ccagccttga ggatggcgag ctcgtctaca acgtcaaggt
     9601 cagaggggta aactttccct ccaatggtcc cgtgatgcag aagaagacca agggttggga
     9661 gcctaataca gagatgatgt atccagcaga tggtggtctg agaggataca ctgacatcgc
     9721 actgaaagtt gatggtggtg gccatctgca ctgcaacttc gtgacaactt acaggtcaaa
     9781 aaagaccgtc gggaacatca agatgcccgg tgtccatgcc gttgatcacc gcctggaaag
     9841 gatcgaggag agtgacaatg aaacctacgt agtgcaaaga gaagtggcag ttgccaaata
     9901 cagcaacctt ggtggtggca tggacgagct gtacaagtaa tagtaaacgc tagcgtttaa
     9961 accttcaggg tgagtctatg ggacgcttga tgttttcttt ccccttcttt tctatggtta
    10021 agttcatgtc ataggaaggg gataagtaac agggtacagt ttagaatggg aaacagacga
    10081 atgattgcat cagtgtggaa gtctcaggat cgttttagtt tcttttattt gctgttcata
    10141 acaattgttt tcttttgttt aattcttgct ttcttttttt ttcttctccg caatttttac
    10201 tattatactt aatgccttaa cattgtgtat aacaaaagga aatatctctg agatacatta
    10261 agtaacttaa aaaaaaactt tacacagtct gcctagtaca ttactatttg gaatatatgt
    10321 gtgcttattt gcatattcat aatctcccta ctttattttc ttttattttt aattgataca
    10381 taatcattat acatatttat gggttaaagt gtaatgtttt aatatgtgta cacatattga
    10441 ccaaatcagg gtaattttgc atttgtaatt ttaaaaaatg ctttcttctt ttaatatact
    10501 tttttgttta tcttatttct aatactttcc ctaatctctt tctttcaggg caataatgat
    10561 acaatgtatc atgcctcttt gcaccattct aaagaataac agtgataatt tctgggttaa
    10621 ggcaatagca atatctctgc atataaatat ttctgcatat aaattgtaac tgatgtaaga
    10681 ggtttcatat tgctaatagc agctacaatc cagctaccat tctgctttta ttttatggtt
    10741 gggataaggc tggattattc tgagtccaag ctaggccctt ttgctaatca tgttcatacc
    10801 tcttatcttc ctcccacagc tcctgggcaa cgtgctgttt aaacccgctg atcagcctcg
    10861 agctagcgtc gactaatgat aagtttaaac gctagcgtcg acaatcaacc tctggattac
    10921 aaaatttgtg aaagattgac tggtattctt aactatgttg ctccttttac gctatgtgga
    10981 tacgctgctt taatgccttt gtatcatgct attgcttccc gtatggcctt cattttctcc
    11041 tccttgtata aatcctggtt gctgtctctt tatgaggagt tgtggcccgt tgtcaggcaa
    11101 cgtggcgtgg tgtgcactgt gtttgctgac gcaaccccca ctggttgggg cattgccacc
    11161 acctgtcagc tcctttccgg gactttcgct ttccccctcc ctattgccac ggcggaactc
    11221 atcgccgcct gccttgcccg ctgctggaca ggggctcggc tgttgggcac tgacaattcc
    11281 gtggtgttgt cggggaagct gacgtccttt ccatggctgc tcgcctgtgt tgccacctgg
    11341 attctgcgcg ggacgtcctt ctgctacgtc ccttcggccc tcaatccagc ggaccttcct
    11401 tcccgcggcc tgctgccggc tctgcggcct cttccgcgtc ttcgccttcg ccctcagacg
    11461 agtcggatct ccctttgggc cgcctccccg cctgtaatct agatagtcag tttaaacccg
    11521 ctgatcagcc tcgactgtgc cttctagttg ccagccatct gttgtttgcc cctcccccgt
    11581 gccttccttg accctggaag gtgccactcc cactgtcctt tcctaataaa atgaggaaat
    11641 tgcatcgcat tgtctgagta ggtgtcattc tattctgggg ggtggggtgg ggcaggacag
    11701 caaggggggg gattgggaag acaatagcag gcatgctggg gatgcggtgg gctctatggc
    11761 ttctctcgag gtcaggcaac atcacccctt ccctgggtag gtgtgtgggt ggaggggctg
    11821 tgggttcctt agcctctctc ctaagccaaa cccagcaaac ggctgccttg gcaacccctc
    11881 agggatgaca gcactgccat gctctctggc aggcataatg ttgccactgt gcctgaggcc
    11941 aacaccctgc gtcgggctgc aaacatccat tcccttccct gtggggaggg aggctctggg
    12001 ggccttagtg ggagactctg gacagggcca agagactgtt gtatgcacac tgcctccagc
    12061 ctgtcaagaa ggcggcgtgc ctggcatccc ttctactggt gattggtgca gatcccttag
    12121 ctttttaaag cttccttgtt ttgtctgatc acacacagca gagctgccct gtatttggca
    12181 gttggcagac agacccatca ctccccacca tgtccacagt cacttgtgca tcctttccta
    12241 taacatcctt gtcaggagct tggtattaga gggagttgtt taagagtggc atagaaagcc
    12301 cccatattat ccttcccaag gtcttgggac agggtgggaa atgttcatct taaatttgta
    12361 aaatggcatc attagtacag ggtgaagaag gtgactcaag tagtcaaggt ggattgaggt
    12421 caggaatctg tctataccag attggtcctg ggcattttgg tggatggatg tggggcttgc
    12481 actgtgtggt tgagaggcct tataaggttg ccctcctgga ggtaccctgg gcctcatggg
    12541 ccttccgctc actgcccgct ttccagtcgg gaaacctgtc cccccggtca ggcaacatca
    12601 ccc
//
