LOCUS       sequence-49598-e         841 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 841)
  AUTHORS   Ritchie TK, Grinkova YV, Bayburt TH, Denisov IG, Zolnerciks JK, 
            Atkins WM, Sligar SG
  TITLE     Chapter 11 - Reconstitution of membrane proteins in phospholipid 
            bilayer nanodiscs.
  JOURNAL   Methods Enzymol. 2009;464:211-31. doi: 
            10.1016/S0076-6879(09)64011-8.
  PUBMED    19903557
REFERENCE   2  (bases 1 to 841)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Methods 
            Enzymol."; date: "2009"; volume: "464:211-31. doi"; pages: " 
            10.1016/S0076-6879(09)64011-8"
COMMENT     Sequence Label: pMSP1E3D1_D73C
FEATURES             Location/Qualifiers
     source          1..841
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             10..27
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             49..69
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
ORIGIN
        1 accatgggtc atcatcatca tcatcacgat tatgatattc ctactactga gaatttgtat
       61 tttcagggtt ctaccttcag taaacttcgc gaacaactgg gccccgtgac gcaggaattt
      121 tggtgcaacc tggaaaaaga aaccgaggga ctgcgtcagg aaatgtccaa agatttagaa
      181 gaggtgaagg ccaaggttca gccatatctc gatgactttc agaaaaaatg gcaggaagag
      241 atggaattat atcgtcaaaa ggtggaaccg ctgcgtgcgg aactgcaaga gggggcacgc
      301 caaaaactcc atgagctcca agagaagctc agcccattag gcgaagaaat gcgcgatcgc
      361 gcccgtgcac atgttgatgc actccggact catttggcgc catatctcga tgactttcag
      421 aaaaaatggc aggaagagat ggaattatat cgtcaaaagg tggaaccgct gcgtgcggaa
      481 ctgcaagagg gggcacgcca aaaactccat gagctccaag agaagctcag cccattaggc
      541 gaagaaatgc gcgatcgcgc ccgtgcacat gttgatgcac tccggactca tttggcgccg
      601 tattcggatg aacttcgcca gcgtttggcc gcacgtctcg aggcgctgaa agaaaacggg
      661 ggtgcccgct tggctgagta ccacgcgaaa gcgacagaac acctgagcac cttgagcgaa
      721 aaagcgaaac cggcgctgga agatctacgc cagggcttat tgcctgttct tgagagcttt
      781 aaagtcagtt ttctgtcagc tctggaagaa tatactaaaa agctgaatac ccagtaagct
      841 t
//
