LOCUS       sequence-495987-        9572 bp    DNA     circular SYN 23-DEC-2025
DEFINITION  Double Death Trap (DDT) Wnt reporter Containing 
            FKBP12(F36V)-Delta-Caspase9 (FC) and Puromycin Resistance Gene for 
            Assessing Wnt Signaling.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9572)
  AUTHORS   He Z, Chen S, Suo J, Xia K, Liu M, Ma J, Chu Y, Wang C, Xie Y, Jiang
            W, Du H, Chen S, Zhou Z, Li M, Wei Q, Zhao Y, Chen J, Li L, Zeng Y, 
            Zou W, Lin M, Jiang H
  TITLE     STT3A is essential for Wnt signaling and represents a target for 
            cancers driven by RNF43 deficiency.
  JOURNAL   Cell Chem Biol. 2025 Oct 22:S2451-9456(25)00306-X. doi: 
            10.1016/j.chembiol.2025.10.001.
  PUBMED    41130209
REFERENCE   2  (bases 1 to 9572)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 23, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell Chem 
            Biol. 2025 Oct 22:S2451-9456(25)00306-X. doi: 
            10.1016/j.chembiol.2025.10.001."
COMMENT     Sequence Label: DDT Wnt
FEATURES             Location/Qualifiers
     source          1..9572
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        41..72
                     /label=minP
                     /note="minimal TATA-box promoter with low basal activity"
     CDS             join(142..144,145..465)
                     /codon_start=1
                     /product="F36V mutant of FK506-binding protein FKBP12"
                     /label=FKBP-V
                     /note="binds synthetic ligands that do not bind wild-type 
                     FKBP"
                     /translation="MGVQVETISPGDGRTFPKRGQTCVVHYTGMLEDGKKVDSSRDRNK
                     PFKFMLGKQEVIRGWEEGVAQMSVGQRAKLTISPDYAYGATGHPGIIPPHATLVFDVEL
                     LKLE"
     CDS             complement(808..819)
                     /codon_start=1
                     /product="WELQut protease recognition and cleavage site"
                     /label=WELQut site
                     /translation="WELQ"
     CDS             1336..1392
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             1393..1989
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="TEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIERV
                     TELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLAA
                     QQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETSA
                     PRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     1886..1906
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     enhancer        2053..2356
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        2357..2560
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     2511..2531
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     CDS             2599..3309
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(2744..2762)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     3012..3031
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     misc_feature    3316..3904
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(3369..3389)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(3787..3798)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             3991..4224
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    4296..4417
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(4333..4352)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     4387..4406
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      4457..4592
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(4612..4631)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(4613..4631)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(4641..4658)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(4641..4657)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4650..4672)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      4799..5254
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(4886..4905)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     5096..5117
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        5280..5384
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(5385..5453,5454..6245)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(5603..5622)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      6416..7004
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6905..6924
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     7158..7175
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    7292..7313
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(7328..7333,7334..7351,7352..7358)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7366..7382
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     7371..7393
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     7390..7406
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     7390..7406
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     7425..7445
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        7427..7445
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        7473..7699
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             7700..7880
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    7927..8052
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    8545..8778
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             8963..9007
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    9274..9391
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
ORIGIN
        1 ggcgcgcccg cgtgctagcc cgggctcgag atctagactc tagagggtat ataatggaag
       61 ctcgaattcc agcttggcat tccggtactg ttggtaaaaa gcttggcatt ccggtactgt
      121 tggtaaagcc accttaatta aatgggagtg caggtggaaa ccatctcccc aggagacggg
      181 cgcaccttcc ccaagcgcgg ccagacctgc gtggtgcact acaccgggat gcttgaagat
      241 ggaaagaaag ttgattcctc ccgggacaga aacaagccct ttaagtttat gctaggcaag
      301 caggaggtga tccgaggctg ggaagaaggg gttgcccaga tgagtgtggg tcagagagcc
      361 aaactgacta tatctccaga ttatgcctat ggtgccactg ggcacccagg catcatccca
      421 ccacatgcca ctctcgtctt cgatgtggag cttctaaaac tggaatctgg cggtggatcc
      481 ggatttggtg atgtcggtgc tcttgagagt ttgaggggaa atgcagattt ggcttacatc
      541 ctgagcatgg agccctgtgg ccactgcctc attatcaaca atgtgaactt ctgccgtgag
      601 tccgggctcc gcacccgcac tggctccaac atcgactgtg agaagttgcg gcgtcgcttc
      661 tcctcgctgc atttcatggt ggaggtgaag ggcgacctga ctgccaagaa aatggtgctg
      721 gctttgctgg agctggcgca gcaggaccac ggtgctctgg actgctgcgt ggtggtcatt
      781 ctctctcacg gctgtcaggc cagccacctg cagttcccag gggctgtcta cggcacagat
      841 ggatgccctg tgtcggtcga gaagattgtg aacatcttca atgggaccag ctgccccagc
      901 ctgggaggga agcccaagct ctttttcatc caggcctgtg gtggggagca gaaagaccat
      961 gggtttgagg tggcctccac ttcccctgaa gacgagtccc ctggcagtaa ccccgagcca
     1021 gatgccaccc cgttccagga aggtttgagg accttcgacc agctggacgc catatctagt
     1081 ttgcccacac ccagtgacat ctttgtgtcc tactctactt tcccaggttt tgtttcctgg
     1141 agggacccca agagtggctc ctggtacgtt gagaccctgg acgacatctt tgagcagtgg
     1201 gctcactctg aagacctgca gtccctcctg cttagggtcg ctaatgctgt ttcggtgaaa
     1261 gggatttata aacagatgcc tggttgcttt aatttcctcc ggaaaaaact tttctttaaa
     1321 acatcaggat ccggcgcaac aaacttctct ctgctgaaac aagccggaga tgtcgaagag
     1381 aatcctggac cgaccgagta caagcccacg gtgcgcctcg ccacccgcga cgacgtcccc
     1441 agggccgtac gcaccctcgc cgccgcgttc gccgactacc ccgccacgcg ccacaccgtc
     1501 gatccggacc gccacatcga gcgggtcacc gagctgcaag aactcttcct cacgcgcgtc
     1561 gggctcgaca tcggcaaggt gtgggtcgcg gacgacggcg ccgcggtggc ggtctggacc
     1621 acgccggaga gcgtcgaagc gggggcggtg ttcgccgaga tcggcccgcg catggccgag
     1681 ttgagcggtt cccggctggc cgcgcagcaa cagatggaag gcctcctggc gccgcaccgg
     1741 cccaaggagc ccgcgtggtt cctggccacc gtcggagtct cgcccgacca ccagggcaag
     1801 ggtctgggca gcgccgtcgt gctccccgga gtggaggcgg ccgagcgcgc cggggtgccc
     1861 gccttcctgg agacctccgc gccccgcaac ctccccttct acgagcggct cggcttcacc
     1921 gtcaccgccg acgtcgaggt gcccgaagga ccgcgcacct ggtgcatgac ccgcaagccc
     1981 ggtgcctgat gatcattcga aatagtaatc aattacgggg tcattagttc atagcccata
     2041 tatggagttc cgcgttacat aacttacggt aaatggcccg cctggctgac cgcccaacga
     2101 cccccgccca ttgacgtcaa taatgacgta tgttcccata gtaacgccaa tagggacttt
     2161 ccattgacgt caatgggtgg agtatttacg gtaaactgcc cacttggcag tacatcaagt
     2221 gtatcatatg ccaagtacgc cccctattga cgtcaatgac ggtaaatggc ccgcctggca
     2281 ttatgcccag tacatgacct tatgggactt tcctacttgg cagtacatct acgtattagt
     2341 catcgctatt accatggtga tgcggttttg gcagtacatc aatgggcgtg gatagcggtt
     2401 tgactcacgg ggatttccaa gtctccaccc cattgacgtc aatgggagtt tgttttggca
     2461 ccaaaatcaa cgggactttc caaaatgtcg taacaactcc gccccattga cgcaaatggg
     2521 cggtaggcgt gtacggtggg aggtctatat aagcagagct ggtttagtga accgtcagat
     2581 ccgctagcgc taccggtcat ggtgagcaag ggcgaggagg ataacatggc catcatcaag
     2641 gagttcatgc gcttcaaggt gcacatggag ggctccgtga acggccacga gttcgagatc
     2701 gagggcgagg gcgagggccg cccctacgag ggcacccaga ccgccaagct gaaggtgacc
     2761 aagggtggcc ccctgccctt cgcctgggac atcctgtccc ctcagttcat gtacggctcc
     2821 aaggcctacg tgaagcaccc cgccgacatc cccgactact tgaagctgtc cttccccgag
     2881 ggcttcaagt gggagcgcgt gatgaacttc gaggacggcg gcgtggtgac cgtgacccag
     2941 gactcctccc tgcaggacgg cgagttcatc tacaaggtga agctgcgcgg caccaacttc
     3001 ccctccgacg gccccgtaat gcagaagaag accatgggct gggaggcctc ctccgagcgg
     3061 atgtaccccg aggacggcgc cctgaagggc gagatcaagc agaggctgaa gctgaaggac
     3121 ggcggccact acgacgctga ggtcaagacc acctacaagg ccaagaagcc cgtgcagctg
     3181 cccggcgcct acaacgtcaa catcaagttg gacatcacct cccacaacga ggactacacc
     3241 atcgtggaac agtacgaacg cgccgagggc cgccactcca ccggcggcat ggacgagctg
     3301 tacaagtaag tcgacaatca acctctggat tacaaaattt gtgaaagatt gactggtatt
     3361 cttaactatg ttgctccttt tacgctatgt ggatacgctg ctttaatgcc tttgtatcat
     3421 gctattgctt cccgtatggc tttcattttc tcctccttgt ataaatcctg gttgctgtct
     3481 ctttatgagg agttgtggcc cgttgtcagg caacgtggcg tggtgtgcac tgtgtttgct
     3541 gacgcaaccc ccactggttg gggcattgcc accacctgtc agctcctttc cgggactttc
     3601 gctttccccc tccctattgc cacggcggaa ctcatcgccg cctgccttgc ccgctgctgg
     3661 acaggggctc ggctgttggg cactgacaat tccgtggtgt tgtcggggaa gctgacgtcc
     3721 tttccatggc tgctcgcctg tgttgccacc tggattctgc gcgggacgtc cttctgctac
     3781 gtcccttcgg ccctcaatcc agcggacctt ccttcccgcg gcctgctgcc ggctctgcgg
     3841 cctcttccgc gtcttcgcct tcgccctcag acgagtcgga tctccctttg ggccgcctcc
     3901 ccgcctggaa ttcgagctcg gtacctttaa gaccaatgac ttacaaggca gctgtagatc
     3961 ttagccactt tttaaaagaa aaggggggac tggaagggct aattcactcc caacgaagac
     4021 aagatctgct ttttgcttgt actgggtctc tctggttaga ccagatctga gcctgggagc
     4081 tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct tgagtgcttc
     4141 aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc agaccctttt
     4201 agtcagtgtg gaaaatctct agcagtagta gttcatgtca tcttattatt cagtatttat
     4261 aacttgcaaa gaaatgaata tcagagagtg agaggaactt gtttattgca gcttataatg
     4321 gttacaaata aagcaatagc atcacaaatt tcacaaataa agcatttttt tcactgcatt
     4381 ctagttgtgg tttgtccaaa ctcatcaatg tatcttatca tgtctggctc tagctatccc
     4441 gcccctaact ccgcccatcc cgcccctaac tccgcccagt tccgcccatt ctccgcccca
     4501 tggctgacta atttttttta tttatgcaga ggccgaggcc gcctcggcct ctgagctatt
     4561 ccagaagtag tgaggaggct tttttggagg cctagggacg tacccaattc gccctatagt
     4621 gagtcgtatt acgcgcgctc actggccgtc gttttacaac gtcgtgactg ggaaaaccct
     4681 ggcgttaccc aacttaatcg ccttgcagca catccccctt tcgccagctg gcgtaatagc
     4741 gaagaggccc gcaccgatcg cccttcccaa cagttgcgca gcctgaatgg cgaatgggac
     4801 gcgccctgta gcggcgcatt aagcgcggcg ggtgtggtgg ttacgcgcag cgtgaccgct
     4861 acacttgcca gcgccctagc gcccgctcct ttcgctttct tcccttcctt tctcgccacg
     4921 ttcgccggct ttccccgtca agctctaaat cgggggctcc ctttagggtt ccgatttagt
     4981 gctttacggc acctcgaccc caaaaaactt gattagggtg atggttcacg tagtgggcca
     5041 tcgccctgat agacggtttt tcgccctttg acgttggagt ccacgttctt taatagtgga
     5101 ctcttgttcc aaactggaac aacactcaac cctatctcgg tctattcttt tgatttataa
     5161 gggattttgc cgatttcggc ctattggtta aaaaatgagc tgatttaaca aaaatttaac
     5221 gcgaatttta acaaaatatt aacgcttaca atttaggtgg cacttttcgg ggaaatgtgc
     5281 gcggaacccc tatttgttta tttttctaaa tacattcaaa tatgtatccg ctcatgagac
     5341 aataaccctg ataaatgctt caataatatt gaaaaaggaa gagtatgagt attcaacatt
     5401 tccgtgtcgc ccttattccc ttttttgcgg cattttgcct tcctgttttt gctcacccag
     5461 aaacgctggt gaaagtaaaa gatgctgaag atcagttggg tgcacgagtg ggttacatcg
     5521 aactggatct caacagcggt aagatccttg agagttttcg ccccgaagaa cgttttccaa
     5581 tgatgagcac ttttaaagtt ctgctatgtg gcgcggtatt atcccgtatt gacgccgggc
     5641 aagagcaact cggtcgccgc atacactatt ctcagaatga cttggttgag tactcaccag
     5701 tcacagaaaa gcatcttacg gatggcatga cagtaagaga attatgcagt gctgccataa
     5761 ccatgagtga taacactgcg gccaacttac ttctgacaac gatcggagga ccgaaggagc
     5821 taaccgcttt tttgcacaac atgggggatc atgtaactcg ccttgatcgt tgggaaccgg
     5881 agctgaatga agccatacca aacgacgagc gtgacaccac gatgcctgta gcaatggcaa
     5941 caacgttgcg caaactatta actggcgaac tacttactct agcttcccgg caacaattaa
     6001 tagactggat ggaggcggat aaagttgcag gaccacttct gcgctcggcc cttccggctg
     6061 gctggtttat tgctgataaa tctggagccg gtgagcgtgg gtctcgcggt atcattgcag
     6121 cactggggcc agatggtaag ccctcccgta tcgtagttat ctacacgacg gggagtcagg
     6181 caactatgga tgaacgaaat agacagatcg ctgagatagg tgcctcactg attaagcatt
     6241 ggtaactgtc agaccaagtt tactcatata tactttagat tgatttaaaa cttcattttt
     6301 aatttaaaag gatctaggtg aagatccttt ttgataatct catgaccaaa atcccttaac
     6361 gtgagttttc gttccactga gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag
     6421 atcctttttt tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg
     6481 tggtttgttt gccggatcaa gagctaccaa ctctttttcc gaaggtaact ggcttcagca
     6541 gagcgcagat accaaatact gttcttctag tgtagccgta gttaggccac cacttcaaga
     6601 actctgtagc accgcctaca tacctcgctc tgctaatcct gttaccagtg gctgctgcca
     6661 gtggcgataa gtcgtgtctt accgggttgg actcaagacg atagttaccg gataaggcgc
     6721 agcggtcggg ctgaacgggg ggttcgtgca cacagcccag cttggagcga acgacctaca
     6781 ccgaactgag atacctacag cgtgagctat gagaaagcgc cacgcttccc gaagggagaa
     6841 aggcggacag gtatccggta agcggcaggg tcggaacagg agagcgcacg agggagcttc
     6901 cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc
     6961 gtcgattttt gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg
     7021 cctttttacg gttcctggcc ttttgctggc cttttgctca catgttcttt cctgcgttat
     7081 cccctgattc tgtggataac cgtattaccg cctttgagtg agctgatacc gctcgccgca
     7141 gccgaacgac cgagcgcagc gagtcagtga gcgaggaagc ggaagagcgc ccaatacgca
     7201 aaccgcctct ccccgcgcgt tggccgattc attaatgcag ctggcacgac aggtttcccg
     7261 actggaaagc gggcagtgag cgcaacgcaa ttaatgtgag ttagctcact cattaggcac
     7321 cccaggcttt acactttatg cttccggctc gtatgttgtg tggaattgtg agcggataac
     7381 aatttcacac aggaaacagc tatgaccatg attacgccaa gcgcgcaatt aaccctcact
     7441 aaagggaaca aaagctggag ctgcaagctt aatgtagtct tatgcaatac tcttgtagtc
     7501 ttgcaacatg gtaacgatga gttagcaaca tgccttacaa ggagagaaaa agcaccgtgc
     7561 atgccgattg gtggaagtaa ggtggtacga tcgtgcctta ttaggaaggc aacagacggg
     7621 tctgacatgg attggacgaa ccactgaatt gccgcattgc agagatattg tatttaagtg
     7681 cctagctcga tacataaacg ggtctctctg gttagaccag atctgagcct gggagctctc
     7741 tggctaacta gggaacccac tgcttaagcc tcaataaagc ttgccttgag tgcttcaagt
     7801 agtgtgtgcc cgtctgttgt gtgactctgg taactagaga tccctcagac ccttttagtc
     7861 agtgtggaaa atctctagca gtggcgcccg aacagggact tgaaagcgaa agggaaacca
     7921 gaggagctct ctcgacgcag gactcggctt gctgaagcgc gcacggcaag aggcgagggg
     7981 cggcgactgg tgagtacgcc aaaaattttg actagcggag gctagaagga gagagatggg
     8041 tgcgagagcg tcagtattaa gcgggggaga attagatcgc gatgggaaaa aattcggtta
     8101 aggccagggg gaaagaaaaa atataaatta aaacatatag tatgggcaag cagggagcta
     8161 gaacgattcg cagttaatcc tggcctgtta gaaacatcag aaggctgtag acaaatactg
     8221 ggacagctac aaccatccct tcagacagga tcagaagaac ttagatcatt atataataca
     8281 gtagcaaccc tctattgtgt gcatcaaagg atagagataa aagacaccaa ggaagcttta
     8341 gacaagatag aggaagagca aaacaaaagt aagaccaccg cacagcaagc ggccgctgat
     8401 cttcagacct ggaggaggag atatgaggga caattggaga agtgaattat ataaatataa
     8461 agtagtaaaa attgaaccat taggagtagc acccaccaag gcaaagagaa gagtggtgca
     8521 gagagaaaaa agagcagtgg gaataggagc tttgttcctt gggttcttgg gagcagcagg
     8581 aagcactatg ggcgcagcgt caatgacgct gacggtacag gccagacaat tattgtctgg
     8641 tatagtgcag cagcagaaca atttgctgag ggctattgag gcgcaacagc atctgttgca
     8701 actcacagtc tggggcatca agcagctcca ggcaagaatc ctggctgtgg aaagatacct
     8761 aaaggatcaa cagctcctgg ggatttgggg ttgctctgga aaactcattt gcaccactgc
     8821 tgtgccttgg aatgctagtt ggagtaataa atctctggaa cagatttgga atcacacgac
     8881 ctggatggag tgggacagag aaattaacaa ttacacaagc ttaatacact ccttaattga
     8941 agaatcgcaa aaccagcaag aaaagaatga acaagaatta ttggaattag ataaatgggc
     9001 aagtttgtgg aattggttta acataacaaa ttggctgtgg tatataaaat tattcataat
     9061 gatagtagga ggcttggtag gtttaagaat agtttttgct gtactttcta tagtgaatag
     9121 agttaggcag ggatattcac cattatcgtt tcagacccac ctcccaaccc cgaggggacc
     9181 cgacaggccc gaaggaatag aagaagaagg tggagagaga gacagagaca gatccattcg
     9241 attagtgaac ggatctcgac ggtatcggtt aacttttaaa agaaaagggg ggattggggg
     9301 gtacagtgca ggggaaagaa tagtagacat aatagcaaca gacatacaaa ctaaagaatt
     9361 acaaaaacaa attacaaaaa ttcaaaattt tatcgatcac gagactagcc tcctagcccg
     9421 ggctcgagca gctgaagctt gcatgcctgc agggtaccga gctcttacgc gagatcaaag
     9481 ggggtaagat caaagggggt aagatcaaag gggcgcgaga tcaaaggggg taagatcaaa
     9541 gggggtaaga tcaaaggggg taagatcaaa gg
//
