LOCUS       sequence-496010-       20467 bp    DNA     circular SYN 23-DEC-2025
DEFINITION  for chromosome editing.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 20467)
  AUTHORS   Sun C, Li H, Liu Y, Li Y, Gao R, Shi X, Fei H, Liu J, Liang R, Gao C
  TITLE     Iterative recombinase technologies for efficient and precise genome 
            engineering across kilobase to megabase scales.
  JOURNAL   Cell. 2025 Aug 1:S0092-8674(25)00800-1. doi: 
            10.1016/j.cell.2025.07.011.
  PUBMED    40763736
REFERENCE   2  (bases 1 to 20467)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 23, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell. 2025 
            Aug 1:S0092-8674(25)00800-1. doi: 10.1016/j.cell.2025.07.011."
COMMENT     Sequence Label: pH-SpRY-PCE
FEATURES             Location/Qualifiers
     source          1..20467
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     174..196
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     188..205
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     189..205
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     ncRNA           1355..1422
                     /product="hepatitis delta virus self-cleaving ribozyme 
                     (Ferre-D'Amare and Scott, 2010)"
                     /label=HDV ribozyme
                     /ncRNA_class="ribozyme"
     terminator      1453..1701
                     /label=HSP terminator
                     /note="efficient transcription terminator from the 
                     Arabidopsis thaliana heat shock protein 18.2 gene (Nagaya 
                     et al., 2010)"
     promoter        1724..3715
                     /label=Ubi promoter
                     /note="maize polyubiquitin gene promoter"
     regulatory      3723..3732
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             3736..3762
                     /codon_start=1
                     /product="nuclear localization signal of human c-Myc 
                     proto-oncogene (Dang and Lee, 1988)"
                     /label=c-myc NLS
                     /translation="PAAKRVKLD"
     CDS             3802..3822
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             8146..8166
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             9892..9912
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             11038..11064
                     /codon_start=1
                     /product="nuclear localization signal of human c-Myc 
                     proto-oncogene (Dang and Lee, 1988)"
                     /label=c-myc NLS
                     /translation="PAAKRVKLD"
     CDS             11104..11124
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     primer_bind     complement(11783..11799)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(11783..11799)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(11796..11818)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    11807..11823
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(11831..11837,11838..11855,11856..11861))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    11876..11897
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        12088..12764
                     /label=CaMV 35S promoter (enhanced)
                     /note="cauliflower mosaic virus 35S promoter with a 
                     duplicated enhancer region"
     primer_bind     12701..12721
                     /label=35S promoter
                     /note="CaMV 35S promoter, forward primer"
     CDS             12831..13856
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRSQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     K"
     polyA_signal    13897..14071
                     /label=CaMV poly(A) signal
                     /note="cauliflower mosaic virus polyadenylation signal"
     misc_feature    14149..14173
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     CDS             14598..15392
                     /codon_start=1
                     /gene="aphA-3"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM
                     TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED
                     EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
                     PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
                     FCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     rep_origin      15479..16067
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     15968..15987
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     16221..16238
                     /label=L4440
                     /note="L4440 vector, forward primer"
     misc_feature    16253..16393
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     complement(16319..16338)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     16479..16501
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     rep_origin      16737..16931
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     CDS             complement(16997..18070)
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="MSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
                     QAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
                     KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
                     GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
                     ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
                     LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
                     LVMRYRNLIEGEASAGS"
     CDS             complement(18499..19128)
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
     misc_feature    20429..20453
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
ORIGIN
        1 aaactgaagg cgggaaacga caatctgatc caagctcaag ctgctctagc attcgccatt
       61 caggctgcgc aactgttggg aagggcgatc ggtgcgggcc tcttcgctat tacgccagct
      121 ggcgaaaggg ggatgtgctg caaggcgatt aagttgggta acgccagggt tttcccagtc
      181 acgacgttgt aaaacgacgg ccagtgaatt catggagtca aagattcaaa tagaggacct
      241 aacagaactc gccgtaaaga ctggcgaaca gttcatacag agtctcttac gactcaatga
      301 caagaagaaa atcttcgtca acatggtgga gcacgacaca cttgtctact ccaaaaatat
      361 caaagataca gtctcagaag accaaagggc aattgagact tttcaacaaa gggtaatatc
      421 cggaaacctc ctcggattcc attgcccagc tatctgtcac tttattgtga agatagtgga
      481 aaaggaaggt ggctcctaca aatgccatca ttgcgataaa ggaaaggcca tcgttgaaga
      541 tgcctctgcc gacagtggtc ccaaagatgg acccccaccc acgaggagca tcgtggaaaa
      601 agaagacgtt ccaaccacgt cttcaaagca agtggattga tgtgattggc agacatactg
      661 tcccacaaat gaagatggaa tctgtaaaag aaaacgcgtg aaataatgcg tctgacaaag
      721 gttaggtcgg ctgcctttaa tcaataccaa agtggtccct accacgatgg aaaaactgtg
      781 cagtcggttt ggctttttct gacgaacaaa taagattcgt ggccgacagg tgggggtcca
      841 ccatgtgaag gcatcttcag actccaataa tggagcaatg acgtaagggc ttacgaaata
      901 agtaagggta gtttgggaaa tgtccactca cccgtcagtc tataaatact tagcccctcc
      961 ctcattgtta agggagcaaa atctcagaga gatagtccta gagagagaaa gagagcaagt
     1021 agcctagaag tagtcaaggc ggcgaagtat tcaggcacgt ggccaggaag aagaaaagcc
     1081 aagacgacga aaacaggtaa gagctaagca tctagataag ttgaaaacaa tcttcaaaag
     1141 tcccacatcg cttagataag aaaacgaagc tgagtttata tacagctaga gtcgaagtag
     1201 tgattgaaca aagcaccagt ggtctagtgg tagaatagta ccctgccacg gtacagaccc
     1261 gggttcgatt cccggctggt gcatgagacc ttcggggaat atgagcgtca ggtctcacgc
     1321 ggttctatct agttacgcgt taaaccaact agaaggccgg catggtccca gcctcctcgc
     1381 tggcgccggc tgggcaacat gcttcggcat ggcgaatggg actttttttt gatatctccg
     1441 gggctaattg aatatgaaga tgaagatgaa atatttggtg tgtcaaataa aaagctggtg
     1501 tgcttaagtt tgtgtttttt tcttggcttg ttgtgttatg aatttgtggc tttttctaat
     1561 attaaatgaa tgtaagatct cattataatg aataaacaaa tgtttctata atccattgtg
     1621 aatgttttgt tggatctctt ctgcagcata taactactgt atgtgctatg gtatggacta
     1681 tggaatatga ttaaagataa gaagcttggc gtaatctgca tgcctgcagt gcagcgtgac
     1741 ccggtcgtgc ccctctctag agataatgag cattgcatgt ctaagttata aaaaattacc
     1801 acatattttt tttgtcacac ttgtttgaag tgcagtttat ctatctttat acatatattt
     1861 aaactttact ctacgaataa tataatctat agtactacaa taatatcagt gttttagaga
     1921 atcatataaa tgaacagtta gacatggtct aaaggacaat tgagtatttt gacaacagga
     1981 ctctacagtt ttatcttttt agtgtgcatg tgttctcctt tttttttgca aatagcttca
     2041 cctatataat acttcatcca ttttattagt acatccattt agggtttagg gttaatggtt
     2101 tttatagact aattttttta gtacatctat tttattctat tttagcctct aaattaagaa
     2161 aactaaaact ctattttagt ttttttattt aataatttag atataaaata gaataaaata
     2221 aagtgactaa aaattaaaca aatacccttt aagaaattaa aaaaactaag gaaacatttt
     2281 tcttgtttcg agtagataat gccagcctgt taaacgccgt cgacgagtct aacggacacc
     2341 aaccagcgaa ccagcagcgt cgcgtcgggc caagcgaagc agacggcacg gcatctctgt
     2401 cgctgcctct ggacccctct cgagagttcc gctccaccgt tggacttgct ccgctgtcgg
     2461 catccagaaa ttgcgtggcg gagcggcaga cgtgagccgg cacggcaggc ggcctcctcc
     2521 tcctctcacg gcaccggcag ctacggggga ttcctttccc accgctcctt cgctttccct
     2581 tcctcgcccg ccgtaataaa tagacacccc ctccacaccc tctttcccca acctcgtgtt
     2641 gttcggagcg cacacacaca caaccagatc tcccccaaat ccacccgtcg gcacctccgc
     2701 ttcaaggtac gccgctcgtc ctcccccccc cccctctcta ccttctctag atcggcgttc
     2761 cggtccatgg ttagggcccg gtagttctac ttctgttcat gtttgtgtta gatccgtgtt
     2821 tgtgttagat ccgtgctgct agcgttcgta cacggatgcg acctgtacgt cagacacgtt
     2881 ctgattgcta acttgccagt gtttctcttt ggggaatcct gggatggctc tagccgttcc
     2941 gcagacggga tcgatttcat gatttttttt gtttcgttgc atagggtttg gtttgccctt
     3001 ttcctttatt tcaatatatg ccgtgcactt gtttgtcggg tcatcttttc atgctttttt
     3061 ttgtcttggt tgtgatgatg tggtctggtt gggcggtcgt tctagatcgg agtagaattc
     3121 tgtttcaaac tacctggtgg atttattaat tttggatctg tatgtgtgtg ccatacatat
     3181 tcatagttac gaattgaaga tgatggatgg aaatatcgat ctaggatagg tatacatgtt
     3241 gatgcgggtt ttactgatgc atatacagag atgctttttg ttcgcttggt tgtgatgatg
     3301 tggtgtggtt gggcggtcgt tcattcgttc tagatcggag tagaatactg tttcaaacta
     3361 cctggtgtat ttattaattt tggaactgta tgtgtgtgtc atacatcttc atagttacga
     3421 gtttaagatg gatggaaata tcgatctagg ataggtatac atgttgatgt gggttttact
     3481 gatgcatata catgatggca tatgcagcat ctattcatat gctctaacct tgagtaccta
     3541 tctattataa taaacaagta tgttttataa ttattttgat cttgatatac ttggatgatg
     3601 gcatatgcag cagctatatg tggatttttt tagccctgcc ttcatacgct atttatttgc
     3661 ttggtactgt ttcttttgtc gatgctcacc ctgttgtttg gtgttacttc tgcagcccta
     3721 gggccaccat ggatgcctgc cgccaagaga gtcaagctcg atggcggcaa gaggaccgcc
     3781 gatggcagcg agttcgagtc tcctaagaag aagaggaagg tcgacaagaa gtactcgatc
     3841 ggcctcgata ttgggactaa ctctgttggc tgggccgtga tcaccgacga gtacaaggtg
     3901 ccctcaaaga agttcaaggt cctgggcaac accgatcggc attccatcaa gaagaatctc
     3961 attggcgctc tcctgttcga cagcggcgag acggctgagc gtacgcggct caagcgcacc
     4021 gcccgcaggc ggtacacgcg caggaagaat cgcatctgct acctgcagga gattttctcc
     4081 aacgagatgg cgaaggttga cgattctttc ttccacaggc tggaggagtc attcctcgtg
     4141 gaggaggata agaagcacga gcggcatcca atcttcggca acattgtcga cgaggttgcc
     4201 taccacgaga agtaccctac gatctaccat ctgcggaaga agctcgtgga ctccacagat
     4261 aaggcggacc tccgcctgat ctacctcgct ctggcccaca tgattaagtt caggggccat
     4321 ttcctgatcg agggggatct caacccggac aatagcgatg ttgacaagct gttcatccag
     4381 ctcgtgcaga cgtacaacca gctcttcgag gagaacccca ttaatgcgtc aggcgtcgac
     4441 gcgaaggcta tcctgtccgc taggctctcg aagtctcgga agctcgagaa cctgatcgcc
     4501 cagctgccgg gcgagaagaa gaacggcctg ttcgggaatc tcattgcgct cagcctgggg
     4561 ctcacgccca acttcaagtc gaatttcgat ctcgctgagg acgccaagct gcagctctcc
     4621 aaggacacat acgacgatga cctggataac ctcctggccc agatcggcga tcagtacgcg
     4681 gacctgttcc tcgctgccaa gaatctgtcg gacgccatcc tcctgtctga tattctcagg
     4741 gtgaacaccg agattacgaa ggctccgctc tcagcctcca tgatcaagcg ctacgacgag
     4801 caccatcagg atctgaccct cctgaaggcg ctggtcaggc agcagctccc cgagaagtac
     4861 aaggagatct tcttcgatca gtcgaagaac ggctacgctg ggtacattga cggcggggcc
     4921 tctcaggagg agttctacaa gttcatcaag ccgattctgg agaagatgga cggcacggag
     4981 gagctgctgg tgaagctcaa gcgcgaggac ctcctgagga agcagcggac attcgataac
     5041 ggcagcatcc cacaccagat tcatctcggg gagctgcacg ctatcctgag gaggcaggag
     5101 gacttctacc ctttcctcaa ggataaccgc gagaagatcg agaagattct gactttcagg
     5161 atcccgtact acgtcggccc actcgctagg ggcaactccc gcttcgcttg gatgacccgc
     5221 aagtcagagg agacgatcac gccgtggaac ttcgaggagg tggtcgacaa gggcgctagc
     5281 gctcagtcgt tcatcgagag gatgacgaat ttcgacaaga acctgccaaa tgagaaggtg
     5341 ctccctaagc actcgctcct gtacgagtac ttcacagtct acaacgagct gactaaggtg
     5401 aagtatgtga ccgagggcat gaggaagccg gctttcctgt ctggggagca gaagaaggcc
     5461 atcgtggacc tcctgttcaa gaccaaccgg aaggtcacgg ttaagcagct caaggaggac
     5521 tacttcaaga agattgagtg cttcgattcg gtcgagatct ctggcgttga ggaccgcttc
     5581 aacgcctccc tggggaccta ccacgatctc ctgaagatca ttaaggataa ggacttcctg
     5641 gacaacgagg agaatgagga tatcctcgag gacattgtgc tgacactcac tctgttcgag
     5701 gaccgggaga tgatcgagga gcgcctgaag acttacgccc atctcttcga tgacaaggtc
     5761 atgaagcagc tcaagaggag gaggtacacc ggctggggga ggctgagcag gaagctcatc
     5821 aacggcattc gggacaagca gtccgggaag acgatcctcg acttcctgaa gagcgatggc
     5881 ttcgcgaacc gcaatttcat gcaactgatc cacgatgaca gcctcacctt caaggaggat
     5941 atccaaaagg ctcaagtgag cggccagggg gactcgctgc acgagcatat cgcgaacctc
     6001 gctggctccc ccgcgatcaa gaagggcatc ctccagaccg tgaaggttgt ggacgagctc
     6061 gtgaaggtca tgggccggca caagcctgag aacatcgtca tcgagatggc cagagagaac
     6121 caaaccacgc agaaggggca aaagaactct agggagcgca tgaagcgcat cgaggagggc
     6181 atcaaggagc tggggtccca aatcctcaag gagcacccag tggagaacac ccaactgcag
     6241 aacgagaagc tctacctgta ctacctccag aacggcaggg atatgtacgt ggaccaagag
     6301 ctggatatca accgcctcag cgattacgac gtcgatgcca tcgttcccca gtctttcctg
     6361 aaggatgact ccatcgacaa caaggtcctc accaggtcgg acaagaaccg cggcaagtca
     6421 gataacgttc catctgagga ggtcgttaag aagatgaaga actactggag gcagctcctg
     6481 aacgccaagc tgatcacgca aaggaagttc gacaacctca ccaaggctga gagaggcggg
     6541 ctctcagagc tggacaaggc cggcttcatc aagcggcagc tggtcgaaac gagacaaatc
     6601 acgaagcacg ttgcgcaaat cctcgactct cggatgaaca cgaagtacga tgagaacgac
     6661 aagctgatca gggaggttaa ggtgatcacc ctgaagtcta agctcgtctc cgacttcagg
     6721 aaggatttcc agttctacaa ggttcgcgag atcaacaact accaccatgc ccatgacgct
     6781 tacctcaacg ctgtggtcgg caccgctctg atcaagaagt acccaaagct ggagtccgag
     6841 ttcgtgtacg gggactacaa ggtttacgat gtgcgcaaga tgatcgccaa gtcggagcaa
     6901 gagatcggca aggctaccgc caagtacttc ttctactcaa acatcatgaa cttcttcaag
     6961 accgagatca cgctggccaa cggcgagatc cggaagcggc ccctgatcga aacgaacggc
     7021 gagacggggg agatcgtgtg ggacaagggc agggatttcg cgaccgtccg caaggttctc
     7081 tccatgcccc aggtgaacat cgtcaagaag accgaggtcc aaacgggcgg gttctcaaag
     7141 gagtctatca ggcctaagcg gaacagcgac aagctcatcg ccagaaagaa ggactgggac
     7201 ccaaagaagt acggcgggtt cctctggcct accgtggcct actcggtcct ggttgtggcg
     7261 aaggttgaga agggcaagtc caagaagctc aagagcgtga aggagctcct ggggatcacc
     7321 atcatggaga ggtccagctt cgagaagaac ccaatcgact tcctggaggc caagggctac
     7381 aaggaggtga agaaggacct gatcatcaag ctcccgaagt actctctctt cgagctggag
     7441 aacggcagga agagaatgct ggcttccgct aagcagctcc agaaggggaa cgagctcgcg
     7501 ctgccaagca agtacgtgaa cttcctctac ctggcttccc actacgagaa gctcaagggc
     7561 agcccggagg acaacgagca aaagcagctg ttcgtcgagc agcacaagca ttacctcgac
     7621 gagatcatcg agcaaatctc cgagttcagc aagcgcgtga tcctcgccga cgcgaacctg
     7681 gataaggtcc tctccgccta caacaagcac cgggacaagc ccatcagaga gcaagcggag
     7741 aacatcatcc atctcttcac cctgacgagg ctcggcgctc ctagggcttt caagtacttc
     7801 gacaccacga tcgatcccaa gcaatacagg tccacgaagg aggtcctgga cgcgaccctc
     7861 atccaccagt cgatcaccgg cctgtacgag acgaggatcg acctctcaca actcggcggg
     7921 gatgaattct ccgggagcga gacgccaggc acctccgagt cggccacccc agaatctgcc
     7981 acagtggtgt ccggccaaaa gcaggaccgc cagggcggag aacgcagaag gtcccagctc
     8041 gatagggatc agtgtgccta ctgcaaggag aagggccact gggccaaaga ctgcccgaaa
     8101 aagccgcgcg gcccacgcgg cccaaggcca caaacatccc tccttccaaa gaagaagcgg
     8161 aaggtggagc tcagcggagg atcttccgga ggatctagcg gctccgagac accaggaaca
     8221 tccgaaagcg ctacaccaga atctagcgga ggctcttccg gaggatctag gcctaccctc
     8281 aacatcgagg atgagtatcg cctccacgaa acctccaaag aaccggacgt gtccctcggc
     8341 agcacatggc tcagcgactt cccacaagcg tgggccgaaa ccggcggcat gggcctcgcc
     8401 gtccgccaag ccccactcat tatcccgctg aaggcgacct ccacaccggt gtccatcaag
     8461 cagtacccga tgagccaaga ggcgaggctc gggattaagc cgcacattca gcgcctcctc
     8521 gatcaaggca ttctcgtgcc gtgccaatcc ccgtggaata caccactcct cccggtcaaa
     8581 aagccgggca ccaacgacta tcgcccggtc caagatctcc gcgaggtcaa caagcgcgtg
     8641 gaagacatcc acccgaccgt cccgaacccg tataatctgc tctccgggct cccaccatcc
     8701 caccagtggt atacagtgct ggacctcaaa gacgccttct tctgtctccg cctccaccca
     8761 acaagccagc cgctcttcgc cttcgagtgg cgcgacccgg agatgggcat ctccggccaa
     8821 ctgacatgga cacgcctccc gcaaggcttc aagaacagcc cgacactctt caacgaggcg
     8881 ctccataggg acctcgcgga ttttcgcatc cagcatccgg acctcatcct cctccagtat
     8941 gcggatgatc tcctcctcgc cgcgacctcc gagctggatt gtcaacaagg cacacgcgcg
     9001 ctcctccaaa cactcgggaa cctcggctat cgcgcgtccg cgaaaaaggc ccaaatctgc
     9061 cagaagcaag tgaagtacct cgggtatctg ctcaaggaag gccaacgctg gctcaccgaa
     9121 gcgcgcaaag aaacagtgat ggggcaaccg acaccgaaaa caccacgcca gctgcgcgag
     9181 tttctcggca aagccggctt ctgtcgcctc ttcatcccgg gctttgccga gatggccgcg
     9241 ccactctacc cactcaccaa gccgggcaca ctgtttaact gggggccgga tcagcagaaa
     9301 gcctaccaag agatcaaaca agcgctcctc accgccccag cgctcgggct cccagatctc
     9361 acaaagccgt tcgagctgtt cgtcgatgag aagcaaggct acgcgaaggg cgtgctcaca
     9421 cagaagctcg gcccgtggag gaggccagtg gcctatctct ccaaaaaact cgatccagtg
     9481 gccgccggct ggccaccgtg tctgcgcatg gtcgccgcga ttgccgtgct cacaaaggat
     9541 gccggcaaac tcacaatggg ccagccgctg gtgatcctcg cgccacatgc cgtggaagcc
     9601 ctcgtcaaac agccgccgga taggtggctc tccaatgcgc gcatgaccca ttaccaagcg
     9661 ctcctcctcg acaccgatcg cgtccagttc ggcccagtgg tcgccctcaa tccggcgaca
     9721 ctgctgccac tcccagagga gggcctccaa cacaactgtc tggatattct cgcggaagcg
     9781 catggcacaa ggccagacct cacagatcaa ccgctcagcg gcggcagcaa gaggaccgcc
     9841 gactcccagc actcgacccc gccgaagacc aagaggaagg tggagttcga gccgaagaag
     9901 aagaggaaag tgagcggcgg atcttccgga ggatctagcg gctccgagac accaggaaca
     9961 tccgaatccg ctacaccaga gtcttctgga ggatctagcg gaggatctat gagtaatctc
    10021 ttgaccgttc atcaaaattt accggcgctc ccggtggacg ccacaagcga tgaggtgcgg
    10081 aagaacctga tggatatgtt ccgtgatcgg caggcgttta gcgaagagac atggaagatg
    10141 cttctttccg tgtgtcgtag ttgggcagcg tggtgcaagc tcaacaaccg gaagtggttc
    10201 cctgctgagc cggaagacgt cagggactat ttactctacc tccaagctag ggggttagca
    10261 gttaaaacta tacagcagca tctcggtcaa ttgaacatgc tgcaccggcg cagcggactg
    10321 ccgcggccca gtgactctaa tgctgtgtcg ctcgtgatga ggcgcatcag aaaggagaat
    10381 gtggatgctg gtgaacgagc caagcaggcg cttgccttcg agaggacaca gtttgatcaa
    10441 gttcgcagcc tcatggagaa cagcgatcgt tgccaagaca tcaggaatct agccttcctg
    10501 ggtatcgcct acaatactct gttgagaatt gcggagattg cgaggatccg tgtgaaggac
    10561 ataagccgaa ctgatggtgg gaggatgttg attcacattg gacgcaccaa gacactcgta
    10621 tcaaccgcag gagtcgagaa ggctctatcg cttggggtta ctaagttggt cgaacgatgg
    10681 atatcagtat ccggcgtcgc cgacgatcca aacaattacc tgttctgccg cgtgcgcaag
    10741 aatggggttg cggcgccttc agctacgtcc cagctctcga cgagggcact ggagggcatc
    10801 tttgaggcta cgcacaggct gatctatggc ccgccggatg attccggcgg gagatacttg
    10861 gcatggtctg gacactccgc cagagtcggc gccgcccgcg acatggcgcg ggccggcgtg
    10921 tcaattccag aaataatgca ggcaggaggg tggaccaatg tcaacattgt aatgaactat
    10981 atcagaaacc tcgactcgga gacaggagcc atggtcaggc ttctagaaga cggtgaccca
    11041 gcagctaaac gcgtaaagtt ggatggaggt aagaggacag cggatggatc tgaattcgag
    11101 agcccgaaaa agaagaggaa agtctgagag ctcagagctt tcgttcgtat catcggtttc
    11161 gacaacgttc gtcaagttca atgcatcagt ttcattgcgc acacaccaga atcctactga
    11221 gtttgagtat tatggcattg ggaaaactgt ttttcttgta ccatttgttg tgcttgtaat
    11281 ttactgtgtt ttttattcgg ttttcgctat cgaactgtga aatggaaatg gatggagaag
    11341 agttaatgaa tgatatggtc cttttgttca ttctcaaatt aatattattt gttttttctc
    11401 ttatttgttg tgtgttgaat ttgaaattat aagagatatg caaacatttt gttttgagta
    11461 aaaatgtgtc aaatcgtggc ctctaatgac cgaagttaat atgaggagta aaacacttgt
    11521 agttgtacca ttatgcttat tcactaggca acaaatatat tttcagacct agaaaagctg
    11581 caaatgttac tgaatacaag tatgtcctct tgtgttttag acatttatga actttccttt
    11641 atgtaatttt ccagaatcct tgtcagattc taatcattgc tttataatta tagttatact
    11701 catggatttg tagttgagta tgaaaatatt ttttaatgca ttttatgact tgccaattga
    11761 ttgacaacga attcgtaatc atgtcatagc tgtttcctgt gtgaaattgt tatccgctca
    11821 caattccaca caacatacga gccggaagca taaagtgtaa agcctggggt gcctaatgag
    11881 tgagctaact cacattaatt gcgttgcgct cactgcccgc tttccagtcg ggaaacctgt
    11941 cgtgccagct gcattaatga atcggccaac gcgcggggag aggcggtttg cgtattggct
    12001 agagcagctt gccaacatgg tggagcacga cactctcgtc tactccaaga atatcaaaga
    12061 tacagtctca gaagaccaaa gggctattga gacttttcaa caaagggtaa tatcgggaaa
    12121 cctcctcgga ttccattgcc cagctatctg tcacttcatc aaaaggacag tagaaaagga
    12181 aggtggcacc tacaaatgcc atcattgcga taaaggaaag gctatcgttc aagatgcctc
    12241 tgccgacagt ggtcccaaag atggaccccc acccacgagg agcatcgtgg aaaaagaaga
    12301 cgttccaacc acgtcttcaa agcaagtgga ttgatgtgaa catggtggag cacgacactc
    12361 tcgtctactc caagaatatc aaagatacag tctcagaaga ccaaagggct attgagactt
    12421 ttcaacaaag ggtaatatcg ggaaacctcc tcggattcca ttgcccagct atctgtcact
    12481 tcatcaaaag gacagtagaa aaggaaggtg gcacctacaa atgccatcat tgcgataaag
    12541 gaaaggctat cgttcaagat gcctctgccg acagtggtcc caaagatgga cccccaccca
    12601 cgaggagcat cgtggaaaaa gaagacgttc caaccacgtc ttcaaagcaa gtggattgat
    12661 gtgatatctc cactgacgta agggatgacg cacaatccca ctatccttcg caagaccctt
    12721 cctctatata aggaagttca tttcatttgg agaggacacg ctgaaatcac cagtctctct
    12781 ctacaaatct atctctctcg agctttcgca gatccggggg gcaatgagat atgaaaaagc
    12841 ctgaactcac cgcgacgtct gtcgagaagt ttctgatcga aaagttcgac agcgtctccg
    12901 acctgatgca gctctcggag ggcgaagaat ctcgtgcttt cagcttcgat gtaggagggc
    12961 gtggatatgt cctgcgggta aatagctgcg ccgatggttt ctacaaagat cgttatgttt
    13021 atcggcactt tgcatcggcc gcgctcccga ttccggaagt gcttgacatt ggggagttta
    13081 gcgagagcct gacctattgc atctcccgcc gttcacaggg tgtcacgttg caagacctgc
    13141 ctgaaaccga actgcccgct gttctacaac cggtcgcgga ggctatggat gcgatcgctg
    13201 cggccgatct tagccagacg agcgggttcg gcccattcgg accgcaagga atcggtcaat
    13261 acactacatg gcgtgatttc atatgcgcga ttgctgatcc ccatgtgtat cactggcaaa
    13321 ctgtgatgga cgacaccgtc agtgcgtccg tcgcgcaggc tctcgatgag ctgatgcttt
    13381 gggccgagga ctgccccgaa gtccggcacc tcgtgcacgc ggatttcggc tccaacaatg
    13441 tcctgacgga caatggccgc ataacagcgg tcattgactg gagcgaggcg atgttcgggg
    13501 attcccaata cgaggtcgcc aacatcttct tctggaggcc gtggttggct tgtatggagc
    13561 agcagacgcg ctacttcgag cggaggcatc cggagcttgc aggatcgcca cgactccggg
    13621 cgtatatgct ccgcattggt cttgaccaac tctatcagag cttggttgac ggcaatttcg
    13681 atgatgcagc ttgggcgcag ggtcgatgcg acgcaatcgt ccgatccgga gccgggactg
    13741 tcgggcgtac acaaatcgcc cgcagaagcg cggccgtctg gaccgatggc tgtgtagaag
    13801 tactcgccga tagtggaaac cgacgcccca gcactcgtcc gagggcaaag aaatagagta
    13861 gatgccgacc gggatctgtc gatcgacaag ctcgagtttc tccataataa tgtgtgagta
    13921 gttcccagat aagggaatta gggttcctat agggtttcgc tcatgtgttg agcatataag
    13981 aaacccttag tatgtatttg tatttgtaaa atacttctat caataaaatt tctaattcct
    14041 aaaaccaaaa tccagtacta aaatccagat cccccgaatt aattcggcgt taattcagta
    14101 cattaaaaac gtccgcaatg tgttattaag ttgtctaagc gtcaatttgt ttacaccaca
    14161 atatatcctg ccaccagcca gccaacagct ccccgaccgg cagctcggca caaaatcacc
    14221 actcgataca ggcagcccat cagtccggga cggcgtcagc gggagagccg ttgtaaggcg
    14281 gcagactttg ctcatgttac cgatgctatt cggaagaacg gcaactaagc tgccgggttt
    14341 gaaacacgga tgatctcgcg gagggtagca tgttgattgt aacgatgaca gagcgttgct
    14401 gcctgtgatc accgcggttt caaaatcggc tccgtcgata ctatgttata cgccaacttt
    14461 gaaaacaact ttgaaaaagc tgttttctgg tatttaaggt tttagaatgc aaggaacagt
    14521 gaattggagt tcgtcttgtt ataattagct tcttggggta tctttaaata ctgtagaaaa
    14581 gaggaaggaa ataataaatg gctaaaatga gaatatcacc ggaattgaaa aaactgatcg
    14641 aaaaataccg ctgcgtaaaa gatacggaag gaatgtctcc tgctaaggta tataagctgg
    14701 tgggagaaaa tgaaaaccta tatttaaaaa tgacggacag ccggtataaa gggaccacct
    14761 atgatgtgga acgggaaaag gacatgatgc tatggctgga aggaaagctg cctgttccaa
    14821 aggtcctgca ctttgaacgg catgatggct ggagcaatct gctcatgagt gaggccgatg
    14881 gcgtcctttg ctcggaagag tatgaagatg aacaaagccc tgaaaagatt atcgagctgt
    14941 atgcggagtg catcaggctc tttcactcca tcgacatatc ggattgtccc tatacgaata
    15001 gcttagacag ccgcttagcc gaattggatt acttactgaa taacgatctg gccgatgtgg
    15061 attgcgaaaa ctgggaagaa gacactccat ttaaagatcc gcgcgagctg tatgattttt
    15121 taaagacgga aaagcccgaa gaggaacttg tcttttccca cggcgacctg ggagacagca
    15181 acatctttgt gaaagatggc aaagtaagtg gctttattga tcttgggaga agcggcaggg
    15241 cggacaagtg gtatgacatt gccttctgcg tccggtcgat cagggaggat atcggggaag
    15301 aacagtatgt cgagctattt tttgacttac tggggatcaa gcctgattgg gagaaaataa
    15361 aatattatat tttactggat gaattgtttt agtacctaga atgcatgacc aaaatccctt
    15421 aacgtgagtt ttcgttccac tgagcgtcag accccgtaga aaagatcaaa ggatcttctt
    15481 gagatccttt ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag
    15541 cggtggtttg tttgccggat caagagctac caactctttt tccgaaggta actggcttca
    15601 gcagagcgca gataccaaat actgtccttc tagtgtagcc gtagttaggc caccacttca
    15661 agaactctgt agcaccgcct acatacctcg ctctgctaat cctgttacca gtggctgctg
    15721 ccagtggcga taagtcgtgt cttaccgggt tggactcaag acgatagtta ccggataagg
    15781 cgcagcggtc gggctgaacg gggggttcgt gcacacagcc cagcttggag cgaacgacct
    15841 acaccgaact gagataccta cagcgtgagc tatgagaaag cgccacgctt cccgaaggga
    15901 gaaaggcgga caggtatccg gtaagcggca gggtcggaac aggagagcgc acgagggagc
    15961 ttccaggggg aaacgcctgg tatctttata gtcctgtcgg gtttcgccac ctctgacttg
    16021 agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac gccagcaacg
    16081 cggccttttt acggttcctg gccttttgct ggccttttgc tcacatgttc tttcctgcgt
    16141 tatcccctga ttctgtggat aaccgtatta ccgcctttga gtgagctgat accgctcgcc
    16201 gcagccgaac gaccgagcgc agcgagtcag tgagcgagga agcggaagag cgcctgatgc
    16261 ggtattttct ccttacgcat ctgtgcggta tttcacaccg catatggtgc actctcagta
    16321 caatctgctc tgatgccgca tagttaagcc agtatacact ccgctatcgc tacgtgactg
    16381 ggtcatggct gcgccccgac acccgccaac acccgctgac gcgccctgac gggcttgtct
    16441 gctcccggca tccgcttaca gacaagctgt gaccgtctcc gggagctgca tgtgtcagag
    16501 gttttcaccg tcatcaccga aacgcgcgag gcagggtgcc ttgatgtggg cgccggcggt
    16561 cgagtggcga cggcgcggct tgtccgcgcc ctggtagatt gcctggccgt aggccagcca
    16621 tttttgagcg gccagcggcc gcgataggcc gacgcgaagc ggcggggcgt agggagcgca
    16681 gcgaccgaag ggtaggcgct ttttgcagct cttcggctgt gcgctggcca gacagttatg
    16741 cacaggccag gcgggtttta agagttttaa taagttttaa agagttttag gcggaaaaat
    16801 cgcctttttt ctcttttata tcagtcactt acatgtgtga ccggttccca atgtacggct
    16861 ttgggttccc aatgtacggg ttccggttcc caatgtacgg ctttgggttc ccaatgtacg
    16921 tgctatccac aggaaacaga ccttttcgac ctttttcccc tgctagggca atttgcccta
    16981 gcatctgctc cgtacattag gaaccggcgg atgcttcgcc ctcgatcagg ttgcggtagc
    17041 gcatgactag gatcgggcca gcctgccccg cctcctcctt caaatcgtac tccggcaggt
    17101 catttgaccc gatcagcttg cgcacggtga aacagaactt cttgaactct ccggcgctgc
    17161 cactgcgttc gtagatcgtc ttgaacaacc atctggcttc tgccttgcct gcggcgcggc
    17221 gtgccaggcg gtagagaaaa cggccgatgc cgggatcgat caaaaagtaa tcggggtgaa
    17281 ccgtcagcac gtccgggttc ttgccttctg tgatctcgcg gtacatccaa tcagctagct
    17341 cgatctcgat gtactccggc cgcccggttt cgctctttac gatcttgtag cggctaatca
    17401 aggcttcacc ctcggatacc gtcaccaggc ggccgttctt ggccttcttc gtacgctgca
    17461 tggcaacgtg cgtggtgttt aaccgaatgc aggtttctac caggtcgtct ttctgctttc
    17521 cgccatcggc tcgccggcag aacttgagta cgtccgcaac gtgtggacgg aacacgcggc
    17581 cgggcttgtc tcccttccct tcccggtatc ggttcatgga ttcggttaga tgggaaaccg
    17641 ccatcagtac caggtcgtaa tcccacacac tggccatgcc ggccggccct gcggaaacct
    17701 ctacgtgccc gtctggaagc tcgtagcgga tcacctcgcc agctcgtcgg tcacgcttcg
    17761 acagacggaa aacggccacg tccatgatgc tgcgactatc gcgggtgccc acgtcataga
    17821 gcatcggaac gaaaaaatct ggttgctcgt cgcccttggg cggcttccta atcgacggcg
    17881 caccggctgc cggcggttgc cgggattctt tgcggattcg atcagcggcc gcttgccacg
    17941 attcaccggg gcgtgcttct gcctcgatgc gttgccgctg ggcggcctgc gcggccttca
    18001 acttctccac caggtcatca cccagcgccg cgccgatttg taccgggccg gatggtttgc
    18061 gaccgctcac gccgattcct cgggcttggg ggttccagtg ccattgcagg gccggcaggc
    18121 aacccagccg cttacgcctg gccaaccgcc cgttcctcca cacatggggc attccacggc
    18181 gtcggtgcct ggttgttctt gattttccat gccgcctcct ttagccgcta aaattcatct
    18241 actcatttat tcatttgctc atttactctg gtagctgcgc gatgtattca gatagcagct
    18301 cggtaatggt cttgccttgg cgtaccgcgt acatcttcag cttggtgtga tcctccgccg
    18361 gcaactgaaa gttgacccgc ttcatggctg gcgtgtctgc caggctggcc aacgttgcag
    18421 ccttgctgct gcgtgcgctc ggacggccgg cacttagcgt gtttgtgctt ttgctcattt
    18481 tctctttacc tcattaactc aaatgagttt tgatttaatt tcagcggcca gcgcctggac
    18541 ctcgcgggca gcgtcgccct cgggttctga ttcaagaacg gttgtgccgg cggcggcagt
    18601 gcctgggtag ctcacgcgct gcgtgatacg ggactcaaga atgggcagct cgtacccggc
    18661 cagcgcctcg gcaacctcac cgccgatgcg cgtgcctttg atcgcccgcg acacgacaaa
    18721 ggccgcttgt agccttccat ccgtgacctc aatgcgctgc ttaaccagct ccaccaggtc
    18781 ggcggtggcc catatgtcgt aagggcttgg ctgcaccgga atcagcacga agtcggctgc
    18841 cttgatcgcg gacacagcca agtccgccgc ctggggcgct ccgtcgatca ctacgaagtc
    18901 gcgccggccg atggccttca cgtcgcggtc aatcgtcggg cggtcgatgc cgacaacggt
    18961 tagcggttga tcttcccgca cggccgccca atcgcgggca ctgccctggg gatcggaatc
    19021 gactaacaga acatcggccc cggcgagttg cagggcgcgg gctagatggg ttgcgatggt
    19081 cgtcttgcct gacccgcctt tctggttaag tacagcgata accttcatgc gttccccttg
    19141 cgtatttgtt tatttactca tcgcatcata tacgcagcga ccgcatgacg caagctgttt
    19201 tactcaaata cacatcacct ttttagacgg cggcgctcgg tttcttcagc ggccaagctg
    19261 gccggccagg ccgccagctt ggcatcagac aaaccggcca ggatttcatg cagccgcacg
    19321 gttgagacgt gcgcgggcgg ctcgaacacg tacccggccg cgatcatctc cgcctcgatc
    19381 tcttcggtaa tgaaaaacgg ttcgtcctgg ccgtcctggt gcggtttcat gcttgttcct
    19441 cttggcgttc attctcggcg gccgccaggg cgtcggcctc ggtcaatgcg tcctcacgga
    19501 aggcaccgcg ccgcctggcc tcggtgggcg tcacttcctc gctgcgctca agtgcgcggt
    19561 acagggtcga gcgatgcacg ccaagcagtg cagccgcctc tttcacggtg cggccttcct
    19621 ggtcgatcag ctcgcgggcg tgcgcgatct gtgccggggt gagggtaggg cgggggccaa
    19681 acttcacgcc tcgggccttg gcggcctcgc gcccgctccg ggtgcggtcg atgattaggg
    19741 aacgctcgaa ctcggcaatg ccggcgaaca cggtcaacac catgcggccg gccggcgtgg
    19801 tggtgtcggc ccacggctct gccaggctac gcaggcccgc gccggcctcc tggatgcgct
    19861 cggcaatgtc cagtaggtcg cgggtgctgc gggccaggcg gtctagcctg gtcactgtca
    19921 caacgtcgcc agggcgtagg tggtcaagca tcctggccag ctccgggcgg tcgcgcctgg
    19981 tgccggtgat cttctcggaa aacagcttgg tgcagccggc cgcgtgcagt tcggcccgtt
    20041 ggttggtcaa gtcctggtcg tcggtgctga cgcgggcata gcccagcagg ccagcggcgg
    20101 cgctcttgtt catggcgtaa tgtctccggt tctagtcgca agtattctac tttatgcgac
    20161 taaaacacgc gacaagaaaa cgccaggaaa agggcagggc ggcagcctgt cgcgtaactt
    20221 aggacttgtg cgacatgtcg ttttcagaag acggctgcac tgaacgtcag aagccgactg
    20281 cactatagca gcggaggggt tggatcaaag tactttgatc ccgaggggaa ccctgtggtt
    20341 ggcatgcaca tacaaatgga cgaacggata aaccttttca cgccctttta aatatccgat
    20401 tattctaata aacgctcttt tctcttaggt ttacccgcca atatatcctg tcaaacactg
    20461 atagttt
//
