LOCUS       sequence-496032-       11830 bp    DNA     circular SYN 02-JAN-2026
DEFINITION  Expression of OsTIR1.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11830)
  AUTHORS   Hubbe P, Sharma C, Pajonk O, Peters N, Guschtschin-Schmidt N, 
            Friemel N, Schuck S
  TITLE     An optimised system for rapid auxin-inducible protein degradation in
            budding yeast
  JOURNAL   bioRxiv 2025.09.24.678194
REFERENCE   2  (bases 1 to 11830)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Jan 2, 2026 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "bioRxiv 
            2025.09.24.678194"
COMMENT     Sequence Label: pRS303K-Pgal-OsTIR1(F74G)-GEM
FEATURES             Location/Qualifiers
     source          1..11830
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(25..47)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     147..166
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     promoter        313..500
                     /gene="S. cerevisiae HIS3"
                     /label=HIS3 promoter
     gene            complement(771..2127)
                     /label=kanMX
                     /note="yeast selectable marker conferring kanamycin 
                     resistance (Wach et al., 1994)"
     terminator      complement(771..968)
                     /label=TEF terminator
                     /note="Ashbya gossypii TEF terminator"
     CDS             complement(974..1783)
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin"
                     /translation="MGKEKTHVSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP
                     DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA
                     FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD
                     FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD
                     RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     1697..1716
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     promoter        complement(1784..2127)
                     /label=TEF promoter
                     /note="Ashbya gossypii TEF promoter"
     promoter        2211..2652
                     /gene="S. cerevisiae GAL1"
                     /label=GAL1 promoter
                     /note="inducible promoter, regulated by Gal4"
     protein_bind    2211..2328
                     /label=UAS
                     /bound_moiety="Gal4"
                     /note="upstream activating sequence mediating 
                     Gal4-dependent induction"
     primer_bind     2623..2646
                     /label=GAL1
                     /note="S. cerevisiae GAL1 promoter, forward primer"
     CDS             2692..4419
                     /codon_start=1
                     /product="rice transport inhibitor response 1 protein, 
                     mutated for use in the AID2 system of inducible degradation
                     (Yesbolatova et al., 2020)"
                     /label=OsTIR1(F74G)
                     /note="F-box protein that mediates auxin-dependent 
                     interaction of a ubiquitin ligase with an auxin-inducible 
                     degron (Tan et al., 2007)"
                     /translation="MTYFPEEVVEHIFSFLPAQRDRNTVSLVCKVWYEIERLSRRGVFV
                     GNCYAVRAGRVAARFPNVRALTVKGKPHGADFNLVPPDWGGYAGPWIEAAARGCHGLEE
                     LRMKRMVVSDESLELLARSFPRFRALVLISCEGFSTDGLAAVASHCKLLRELDLQENEV
                     EDRGPRWLSCFPDSCTSLVSLNFACIKGEVNAGSLERLVSRSPNLRSLRLNRSVSVDTL
                     AKILLRTPNLEDLGTGNLTDDFQTESYFKLTSALEKCKMLRSLSGFWDASPVCLSFIYP
                     LCAQLTGLNLSYAPTLDASDLTKMISRCVKLQRLWVLDCISDKGLQVVASSCKDLQELR
                     VFPSDFYVAGYSAVTEEGLVAVSLGCPKLNSLLYFCHQMTNAALVTVAKNCPNFTRFRL
                     CILEPGKPDVVTSQPLDEGFGAIVRECKGLQRLSISGLLTDKVFMYIGKYAKQLEMLSI
                     AFAGDSDKGMMHVMNGCKNLRKLEIRDSPFGDAALLGNFARYETMRSLWMSSCNVTLKG
                     CQVLASKMPMLNVEVINERDGSNEMEENHGDLPKVEKLYVYRTTAGARDDAPNFVKIL"
     terminator      4426..4673
                     /gene="S. cerevisiae CYC1"
                     /label=CYC1 terminator
                     /note="transcription terminator for CYC1"
     primer_bind     complement(4442..4460)
                     /label=CYC1
                     /note="CYC1 transcription termination signal, reverse 
                     primer"
     protein_bind    5260..5278
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    5281..5299
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     CDS             5352..5792
                     /codon_start=1
                     /gene="Saccharomyces cerevisiae GAL4 (truncated)"
                     /product="DNA binding domain of the GAL4 transcriptional 
                     activator"
                     /label=GAL4 DNA binding domain
                     /translation="MKLLSSIEQACDICRLKKLKCSKEKPKCAKCLKNNWECCYSPKTK
                     RSPLTRAHLTEVESRLERLEQLFLLIFPREDLGMILKMDSLQDIKALLTGLFVQDNVNK
                     DAVTDRLASVETDMPLTLRQHRISATSSSEESSNKGQRQLTVS"
     CDS             5838..6773
                     /codon_start=1
                     /product="mutated ligand-binding domain of the human 
                     estrogen receptor (Feil et al., 1997)"
                     /label=ERT2
                     /translation="AGDMRAANLWPSPLMIKRSKKNSLALSLTADQMVSALLDAEPPIL
                     YSEYDPTRPFSEASMMGLLTNLADRELVHMINWAKRVPGFVDLTLHDQVHLLECAWLEI
                     LMIGLVWRSMEHPVKLLFAPNLLLDRNQGKCVEGMVEIFDMLLATSSRFRMMNLQGEEF
                     VCLKSIILLNSGVYTFLSSTLKSLEEKDHIHRVLDKITDTLIHLMAKAGLTLQQQHQRL
                     AQLLLILSHIRHMSNKGMEHLYSMKCKNVVPLYDLLLEMLDAHRLHAPTSRGGASVEET
                     DQSHLATAGSTSSHSLQKYYITGEAEGFPAT"
     CDS             5886..6776
                     /codon_start=1
                     /gene="ESR1"
                     /product="estrogen nuclear receptor alpha ligand-binding 
                     domain"
                     /label=ER-LBD
                     /translation="KRSKKNSLALSLTADQMVSALLDAEPPILYSEYDPTRPFSEASMM
                     GLLTNLADRELVHMINWAKRVPGFVDLTLHDQVHLLECAWLEILMIGLVWRSMEHPVKL
                     LFAPNLLLDRNQGKCVEGMVEIFDMLLATSSRFRMMNLQGEEFVCLKSIILLNSGVYTF
                     LSSTLKSLEEKDHIHRVLDKITDTLIHLMAKAGLTLQQQHQRLAQLLLILSHIRHMSNK
                     GMEHLYSMKCKNVVPLYDLLLEMLDAHRLHAPTSRGGASVEETDQSHLATAGSTSSHSL
                     QKYYITGEAEGFPATV"
     rep_origin      complement(8890..9345)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(9027..9048)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     9239..9258
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     CDS             complement(9322..9609)
                     /codon_start=1
                     /gene="lacZ fragment"
                     /product="LacZ-alpha fragment of beta-galactosidase"
                     /label=lacZ-alpha
                     /translation="MTMITPSSKLTLTKGNKSWYRAPPSNSPYSESYYNSLAVVLQRRD
                     WENPGVTQLNRLAAHPPFASWRNSEEARTDRPSQQLRSLNGEWTRPVAAH"
     primer_bind     9471..9493
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     9485..9502
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     9486..9502
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     9509..9528
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        9509..9527
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     promoter        complement(9566..9584)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(9605..9621)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(9605..9621)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(9618..9640)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    9629..9645
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(9653..9659,9660..9677,9678..9683))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    9698..9719
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(9836..9853)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(10007..10595)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(10087..10106)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(10766..11557,11558..11626))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLXKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTATNLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     11389..11408
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(11627..11731)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     11799..11817
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
ORIGIN
        1 gcgtttcggt gatgacggtg aaaacctctg acacatgcag ctcccggaga cggtcacagc
       61 ttgtctgtaa gcggatgccg ggagcagaca agcccgtcag ggcgcgtcag cgggtgttgg
      121 cgggtgtcgg ggctggctta actatgcggc atcagagcag attgtactga gagtgcacca
      181 taaattcccg ttttaagagc ttggtgagcg ctaggagtca ctgccaggta tcgtttgaac
      241 acggcattag tcagggaagt cataacacag tcctttcccg caattttctt tttctattac
      301 tcttggcctc ctctagtaca ctctatattt ttttatgcct cggtaatgat tttcattttt
      361 ttttttccac ctagcggatg actctttttt tttcttagcg attggcatta tcacataatg
      421 aattatacat tatataaagt aatgtgattt cttcgaagaa tatactaaaa aatgagcagg
      481 caagataaac gaaggcaaag atgacagagc agaaagccct agtaaagcgt attacaaatg
      541 aaaccaagat tcagattgcg atctctttaa agggtggtcc cctagcgata gagcactcga
      601 tcttcccaga aaaagaggca gaagcagtag cagaacaggc cacacaatcg caagtgatta
      661 acgtccacac aggtataggg tttctggacc atatgataca tgctctggat catcgatgaa
      721 ttcgagctcg ttttcgacac tggatggcgg cgttagtatc gaatcgacag cagtatagcg
      781 accagcattc acatacgatt gacgcatgat attactttct gcgcacttaa cttcgcatct
      841 gggcagatga tgtcgaggcg aaaaaaaata taaatcacgc taacatttga ttaaaataga
      901 acaactacaa tataaaaaaa ctatacaaat gacaagttct tgaaaacaag aatcttttta
      961 ttgtcagtac tgattagaaa aactcatcga gcatcaaatg aaactgcaat ttattcatat
     1021 caggattatc aataccatat ttttgaaaaa gccgtttctg taatgaagga gaaaactcac
     1081 cgaggcagtt ccataggatg gcaagatcct ggtatcggtc tgcgattccg actcgtccaa
     1141 catcaataca acctattaat ttcccctcgt caaaaataag gttatcaagt gagaaatcac
     1201 catgagtgac gactgaatcc ggtgagaatg gcaaaagctt atgcatttct ttccagactt
     1261 gttcaacagg ccagccatta cgctcgtcat caaaatcact cgcatcaacc aaaccgttat
     1321 tcattcgtga ttgcgcctga gcgagacgaa atacgcgatc gctgttaaaa ggacaattac
     1381 aaacaggaat cgaatgcaac cggcgcagga acactgccag cgcatcaaca atattttcac
     1441 ctgaatcagg atattcttct aatacctgga atgctgtttt gccggggatc gcagtggtga
     1501 gtaaccatgc atcatcagga gtacggataa aatgcttgat ggtcggaaga ggcataaatt
     1561 ccgtcagcca gtttagtctg accatctcat ctgtaacatc attggcaacg ctacctttgc
     1621 catgtttcag aaacaactct ggcgcatcgg gcttcccata caatcgatag attgtcgcac
     1681 ctgattgccc gacattatcg cgagcccatt tatacccata taaatcagca tccatgttgg
     1741 aatttaatcg cggcctcgaa acgtgagtct tttccttacc catggttgtt tatgttcgga
     1801 tgtgatgtga gaactgtatc ctagcaagat tttaaaagga agtatatgaa agaagaacct
     1861 cagtggcaaa tcctaacctt ttatatttct ctacaggggc gcggcgtggg gacaattcaa
     1921 cgcgtctgtg aggggagcgt ttccctgctc gcaggtctgc agcgaggagc cgtaattttt
     1981 gcttcgcgcc gtgcggccat caaaatgtat ggatgcaaat gattatacat ggggatgtat
     2041 gggctaaatg tacgggcgac agtcacatca tgcccctgag ctgcgcacgt caagactgtc
     2101 aaggagggta ttctgggcct ccatgtcgct ggccgggtga cccggcgggg acgaggcaag
     2161 ctaaacagat ctggcgcgcc ttaattaacc cggggatccg agctctagta cggattagaa
     2221 gccgccgagc gggtgacagc cctccgaagg aagactctcc tccgtgcgtc ctcgtcttca
     2281 ccggtcgcgt tcctgaaacg cagatgtgcc tcgcgccgca ctgctccgaa caataaagat
     2341 tctacaatac tagcttttat ggttatgaag aggaaaaatt ggcagtaacc tggccccaca
     2401 aaccttcaaa tgaacgaatc aaattaacaa ccataggatg ataatgcgat tagtttttta
     2461 gccttatttc tggggtaatt aatcagcgaa gcgatgattt ttgatctatt aacagatata
     2521 taaatgcaaa aactgcataa ccactttaac taatactttc aacattttcg gtttgtatta
     2581 cttcttattc aaatgtaata aaagtatcaa caaaaaattg ttaatatacc tctatacttt
     2641 aacgtcaagg agaaaaaaca gaacttagtt tcgacggatt ctagaactag tatgacttat
     2701 tttcctgaag aagttgtaga acacatattt tcctttttac ctgctcaaag agatagaaac
     2761 accgtctcct tggtctgtaa ggtttggtac gaaatcgaaa gattgtctag aagaggtgtt
     2821 tttgttggta attgttacgc agttagagct ggtagagttg ctgcaagatt cccaaacgtt
     2881 agagcattga ctgttaaagg taaacctcat ggtgctgatt tcaatttggt tccacctgat
     2941 tggggtggtt atgctggtcc atggattgaa gctgcagcta gaggttgtca cggtttggaa
     3001 gaattgagaa tgaagagaat ggttgtttct gatgaatcat tggaattgtt agcaagatct
     3061 tttcctagat tcagagcttt ggttttgatc tcatgtgaag gtttttccac cgatggttta
     3121 gcagctgttg cttcacattg taagttgttg agagaattgg atttgcaaga aaacgaagtt
     3181 gaagatagag gtccaagatg gttatcttgt tttcctgatt cctgtacaag tttggtttct
     3241 ttgaacttcg catgtatcaa gggtgaagtt aacgctggta gtttggaaag attagtttct
     3301 agatcaccaa atttgagatc tttgagattg aacagatccg ttagtgttga taccttggca
     3361 aagatcttgt tgagaactcc taatttggaa gatttgggta ctggtaactt gacagatgat
     3421 ttccaaacag aatcatactt caagttgacc tccgctttgg aaaagtgtaa gatgttgaga
     3481 tctttgtcag gtttttggga tgcaagtcca gtttgtttgt cttttatata tcctttgtgt
     3541 gctcaattga ctggtttgaa tttgtcttac gcaccaactt tggatgctag tgatttgaca
     3601 aagatgatct ctagatgtgt taagttgcaa agattgtggg ttttggattg tatctcagat
     3661 aaaggtttgc aagttgttgc ttcttcttgt aaggatttgc aagaattgag agtttttcca
     3721 agtgatttct atgttgcagg ttactctgct gttacagaag aaggtttagt tgcagtttca
     3781 ttgggttgtc ctaagttgaa ctccttgttg tacttctgtc atcaaatgac caacgcagct
     3841 ttggttactg ttgctaaaaa ttgtccaaac ttcactagat tcagattgtg tatcttggaa
     3901 ccaggtaaac ctgatgttgt tacatcacaa cctttagatg aaggttttgg tgcaatcgtt
     3961 agagagtgta agggtttgca aagattgtcc atcagtggtt tgttgacaga taaggttttt
     4021 atgtacatcg gtaaatacgc taagcaattg gaaatgttgt caatcgcatt cgctggtgac
     4081 tccgataagg gtatgatgca tgttatgaac ggttgtaaga acttaagaaa gttggaaatc
     4141 agagattctc catttggtga cgcagctttg ttgggtaatt tcgcaagata tgaaacaatg
     4201 agatctttgt ggatgtccag ttgtaacgtt accttgaaag gttgtcaagt tttagcttca
     4261 aagatgccaa tgttgaacgt tgaagttatt aacgaaagag atggttccaa tgaaatggaa
     4321 gaaaaccatg gtgacttgcc taaagttgaa aagttatacg tctacagaac aaccgcaggt
     4381 gccagagatg atgctcctaa cttcgtcaag attttatgac tcgagtcatg taattagtta
     4441 tgtcacgctt acattcacgc cctcccccca catccgctct aaccgaaaag gaaggagtta
     4501 gacaacctga agtctaggtc cctatttatt tttttatagt tatgttagta ttaagaacgt
     4561 tatttatatt tcaaattttt cttttttttc tgtacagacg cgtgtacgca tgtaacatta
     4621 tactgaaaac cttgcttgag aaggttttgg gacgctcgaa ggctttaatt tgcggccggt
     4681 cgacctgcag cgtacgcagt ggcaaccaaa cccatacatc gggattccta taataccttc
     4741 gttggtctcc ctaacatgta ggtggcggag gggagatata caatagaaca gataccagac
     4801 aagacataat gggctaaaca agactacacc aattacactg cctcattgat ggtggtacat
     4861 aacgaactaa tactgtagcc ctagacttga tagccatcat catatcgaag tttcactacc
     4921 ctttttccat ttgccatcta ttgaagtaat aataggcgca tgcaacttct tttctttttt
     4981 tttcttttct ctctcccccg ttgttgtctc accatatccg caatgacaaa aaaatgatgg
     5041 aagacactaa aggaaaaatt taacgacaaa gacagcacca acagatgtcg ttgttccaga
     5101 gctgatgagg ggtatctcga agcacacgaa actttttcct tccttcattc acgcacacta
     5161 ctctctaatg agcaacggta tacggccttc cttccagtta cttgaatttg aaataaaaaa
     5221 aagtttgctg tcttgctatc aagtataaat agacctgaat ccctatcagt gatagagatc
     5281 tccctatcag tgatagagat tcttccttgt ttctttttct gcaggtcgac aaacaaatgc
     5341 tcgacataaa aatgaagcta ctgtcttcta tcgaacaagc atgcgatatt tgccgactta
     5401 aaaagctcaa gtgctccaaa gaaaaaccga agtgcgccaa gtgtctgaag aacaactggg
     5461 agtgttgcta ctctcccaaa accaaaaggt ctccgctgac tagggcacat ctgacagaag
     5521 tggaatcaag gctagaaaga ctggaacagc tatttctact gatttttcct cgagaagacc
     5581 ttggcatgat tttgaaaatg gattctttac aggatataaa agcattgtta acaggattat
     5641 ttgtacaaga taatgtgaat aaagatgccg tcacagatag attggcttca gtggagactg
     5701 atatgcctct aacattgaga cagcatagaa taagtgcgac atcatcatcg gaagagagta
     5761 gtaacaaagg tcaaagacag ttgactgtat cgattgactc ggcaggatcc ggtgacggtg
     5821 ctggtttaat taactctgct ggagacatga gagctgccaa cctttggcca agcccgctca
     5881 tgatcaaacg ctctaagaag aacagcctgg ccttgtccct gacggccgac cagatggtca
     5941 gtgccttgtt ggatgctgag ccccccatac tctattccga gtatgatcct accagaccct
     6001 tcagtgaagc ttcgatgatg ggcttactga ccaacctggc agacagggag ctggttcaca
     6061 tgatcaactg ggcgaagagg gtgccaggct ttgtggattt gaccctccat gatcaggtcc
     6121 accttctaga atgtgcctgg ctagagatcc tgatgattgg tctcgtctgg cgctccatgg
     6181 agcacccagt gaagctactg tttgctccta acttgctctt ggacaggaac cagggaaaat
     6241 gtgtagaggg catggtggag atcttcgaca tgctgctggc tacatcatct cggttccgca
     6301 tgatgaatct gcagggagag gagtttgtgt gcctcaaatc tattattttg cttaattctg
     6361 gagtgtacac atttctgtcc agcaccctga agtctctgga agagaaggac catatccacc
     6421 gagtcctgga caagatcaca gacactttga tccacctgat ggccaaggca ggcctgaccc
     6481 tgcagcagca gcaccagcgg ctggcccagc tcctcctcat cctctcccac atcaggcaca
     6541 tgagtaacaa aggcatggag catctgtaca gcatgaagtg caagaacgtg gtgcccctct
     6601 atgacctgct gctggagatg ctggacgccc accgcctaca tgcgcccact agccgtggag
     6661 gggcatccgt ggaggagacg gaccaaagcc acttggccac tgcgggctct acttcatcgc
     6721 attccttgca aaagtattac atcacggggg aggcagaggg tttccctgcc acagtcgcgg
     6781 ccgcaggtga cggtgctggt ttaattaaca tgacggtcga ccatgatttc aatagcgaag
     6841 atattttatt ccccatagaa agcatgagta gtatacaata cgtggagaat aataacccaa
     6901 ataatattaa caacgatgtt atcccgtatt ctctagatat caaaaacact gtcttagata
     6961 gtgcggatct caatgacatt caaaatcaag aaacttcact gaatttgggg cttcctccac
     7021 tatctttcga ctctccactg cccgtaacgg aaacgatacc atccactacc gataacagct
     7081 tgcatttgaa agctgatagc aacaaaaatc gcgatgcaag aactattgaa aatgatagtg
     7141 aaattaagag tactaataat gctagtggct ctggggcaaa tcaatacaca actcttactt
     7201 caccttatcc tatgaacgac attttgtaca acatgaacaa tccgttacaa tcaccgtcac
     7261 cttcatcggt acctcaaaat ccgactataa atcctcccat aaatacagca agtaacgaaa
     7321 ctaatttatc gcctcaaact tcaaatggta atgaaactct tatatctcct cgagcccaac
     7381 aacatacgtc cattaaagat aatcgtctgt ccttacctaa tggtgctaat tcgaatcttt
     7441 tcattgacac taacccaaac aatttgaacg aaaaactaag aaatcaattg aactcagata
     7501 caaattcata ttctaactcc atttctaatt caaactccaa ttctacgggt aatttaaatt
     7561 ccagttattt taattcactg aacatagact ccatgctaga tgattacgtt tctagtgatc
     7621 tcttattgaa tgatgatgat gatgacacta atttatcacg ccgaagattt agcgacgtta
     7681 taacaaacca agagctctaa gcaaatagct aaattatata cgaattaata ttatgattaa
     7741 gtgtttacgt gagtgcgata tttttattac tatcttatac agttgtatat actctataaa
     7801 atgagttgtc tattaattaa cgcgatgaat gctttctggg tttacctctc caacaactct
     7861 agtttacttc tcaatacatt caattgtatt tgatttgtca atacttcatc attaatcaat
     7921 tctatagttt tgtttttctc gtttatttcc aaatttaatg catcaatttt attattcaat
     7981 ttgtcgttga ttttggttaa tgattttatg gtttgatctc tggcattgat tgtttgtgtt
     8041 agtttttcat tattgataat taaattattt aagttagtta tcaactcggt gttttcaagt
     8101 ttcaagtttt caatttcttt agagtttatt agatttgtca aagtttctga attgcttgat
     8161 tggtcctgta gaagagtatt tgttgttgtg gataattgat tcaatttttg agacaattgc
     8221 tggaaggcgt tgaaatatct agcatcaatc tcatggtttt tttcccgaga gtctcgtaga
     8281 ttcaattgtt ttaatatatc ttgggaccac tcttgatttg aactcatgga aattaaactg
     8341 ggtgttgtgt tgtggtgtaa tgattgtacc ccctttgctt ataattgtgt ggtgtcgaac
     8401 ttggtttgca aagggagaaa gtaggagatc tctcttgcga gatgatcccg cattttcttg
     8461 aaagctttgc agaggctagc agaattaccc tccacgttga ttgtctgcga ggcaagaatg
     8521 atcatcaccg tagtgagagt gcgttcaagg ctcttgcggt tgccataaga gaagccacct
     8581 cgcccaatgg taccaacgat gttccctcca ccaaaggtgt tcttatgtag tgacaccgat
     8641 tatttaaagc tgcagcatac gatatatata catgtgtata tatgtatacc tatgaatgtc
     8701 agtaagtatg tatacgaaca gtatgatact gaagatgaca aggtaatgca tcattctata
     8761 cgtgtcattc tgaacgaggc gcgctttcct tttttctttt tgctttttct ttttttttct
     8821 cttgaactcg acggatctat gcggtgtgaa ataccgcaca gatgcgtaag gagaaaatac
     8881 cgcatcagga aattgtaagc gttaatattt tgttaaaatt cgcgttaaat ttttgttaaa
     8941 tcagctcatt ttttaaccaa taggccgaaa tcggcaaaat cccttataaa tcaaaagaat
     9001 agaccgagat agggttgagt gttgttccag tttggaacaa gagtccacta ttaaagaacg
     9061 tggactccaa cgtcaaaggg cgaaaaaccg tctatcaggg cgatggccca ctacgtgaac
     9121 catcacccta atcaagtttt ttggggtcga ggtgccgtaa agcactaaat cggaacccta
     9181 aagggagccc ccgatttaga gcttgacggg gaaagccggc gaacgtggcg agaaaggaag
     9241 ggaagaaagc gaaaggagcg ggcgctaggg cgctggcaag tgtagcggtc acgctgcgcg
     9301 taaccaccac acccgccgcg cttaatgcgc cgctacaggg cgcgtccatt cgccattcag
     9361 gctgcgcaac tgttgggaag ggcgatcggt gcgggcctct tcgctattac gccagctggc
     9421 gaaaggggga tgtgctgcaa ggcgattaag ttgggtaacg ccagggtttt cccagtcacg
     9481 acgttgtaaa acgacggcca gtgaattgta atacgactca ctatagggcg aattggaggg
     9541 gggggcccgg taccagcttt tgttcccttt agtgagggtt aatttcgagc ttggcgtaat
     9601 catggtcata gctgtttcct gtgtgaaatt gttatccgct cacaattcca cacaacatac
     9661 gagccggaag cataaagtgt aaagcctggg gtgcctaatg agtgagctaa ctcacattaa
     9721 ttgcgttgcg ctcactgccc gctttccagt cgggaagcct gtcgtgccag ctgcattaat
     9781 gaatcggcca acgcgcgggg agaggcggtt tgcgtattgg gcgctcttcc gcttcctcgc
     9841 tcactgactc gctgcgctcg gtcgttcggc tgcggcgagc ggtatcagct cactcaaagg
     9901 cggtaatacg gttatccaca gaatcagggg ataacgcagg aaagaacatg tgagcaaaag
     9961 gccagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc
    10021 gcccccctga cgagcatcac aaaaatcgac gctcaagtca gaggtggcga aacccgacag
    10081 gactataaag ataccaggcg tttccccctg gaagctccct cgtgcgctct cctgttccga
    10141 ccctgccgct taccggatac ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc
    10201 atagctcacg ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg
    10261 tgcacgaacc ccccgttcag cccgaccgct gcgccttatc cggtaactat cgtcttgagt
    10321 ccaacccggt aagacacgac ttatcgccac tggcagcagc cactggtaac aggattagca
    10381 gagcgaggta tgtaggcggt gctacagagt tcttgaagtg gtggcctaac tacggctaca
    10441 ctagaagaac agtatttggt atctgcgctc tgctgaagcc agttaccttc ggaaaaagag
    10501 ttggtagctc ttgatccggc aaacaaacca ccgctggtag cggtggtttt tttgtttgca
    10561 agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg
    10621 ggtctgacgc tcagtggaac gaaaactcac gttaagggat tttggtcatg agattatcaa
    10681 aaaggatctt cacctagatc cttttaaatt aaaaatgaag ttttaaatca atctaaagta
    10741 tatatgagta aacttggtct gacagttacc aatgcttaat cagtgaggca cctatctcag
    10801 cgatctgtct atttcgttca tccatagttg cctgactccc cgtcgtgtag ataactacga
    10861 tacgggaggg cttaccatct ggccccagtg ctgcaatgat accgcgagac ccacgctcac
    10921 cggctccaga tttatcagca ataaaccagc cagccggaag ggccgagcgc agaagtggtc
    10981 ctgcaacttt atccgcctcc atccagtcta ttaattgttg ccgggaagct agagtaagta
    11041 gttcgccagt taatagtttg cgcaacgttg ttgccattgc tacaggcatc gtggtgtcac
    11101 gctcgtcgtt tggtatggct tcattcagct ccggttccca acgatcaagg cgagttacat
    11161 gatcccccat gttgtgcaaa aaagcggtta gctccttcgg tcctccgatc gttgtcagaa
    11221 gtaagttggt cgcagtgtta tcactcatgg ttatggcagc actgcataat tctcttactg
    11281 tcatgccatc cgtaagatgc ttttctgtga ctggtgagta ctcaaccaag tcattctgag
    11341 aatagtgtat gcggcgaccg agttgctctt gcccggcgtc aatacgggat aataccgcgc
    11401 cacatagcag aactttaaaa gtgctcatca ttggaaaacg ttcttcgggg cgaaaactct
    11461 caaggatctt accgctgttg agatccagtt cgatgtaacc cactcgtgca cccaactgat
    11521 cttcagcatc ttttactttc wccagcgttt ctgggtgagc aaaaacagga aggcaaaatg
    11581 ccgcaaaaaa gggaataagg gcgacacgga aatgttgaat actcatactc ttcctttttc
    11641 aatattattg aagcatttat cagggttatt gtctcatgag cggatacata tttgaatgta
    11701 tttagaaaaa taaacaaata ggggttccgc gcacatttcc ccgaaaagtg ccacctgacg
    11761 tctaagaaac cattattatc atgacattaa cctataaaaa taggcgtatc acgaggccct
    11821 ttcgtctcgc
//
