LOCUS       sequence-496036-        8634 bp    DNA     circular SYN 02-JAN-2026
DEFINITION  Expression of OsTIR1.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8634)
  AUTHORS   Hubbe P, Sharma C, Pajonk O, Peters N, Guschtschin-Schmidt N, 
            Friemel N, Schuck S
  TITLE     An optimised system for rapid auxin-inducible protein degradation in
            budding yeast
  JOURNAL   bioRxiv 2025.09.24.678194
REFERENCE   2  (bases 1 to 8634)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Jan 2, 2026 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "bioRxiv 
            2025.09.24.678194"
COMMENT     Sequence Label: pRS303H-Padh-OsTIR1(F74G)
FEATURES             Location/Qualifiers
     source          1..8634
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(25..47)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     147..166
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     promoter        313..500
                     /gene="S. cerevisiae HIS3"
                     /label=HIS3 promoter
     terminator      complement(726..973)
                     /gene="S. cerevisiae CYC1"
                     /label=CYC1 terminator
                     /note="transcription terminator for CYC1"
     CDS             complement(981..2009)
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MGKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGR
                     GYVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDL
                     PETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHW
                     QTVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAM
                     FGDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVD
                     GNFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRA
                     KE"
     CDS             complement(981..2009)
                     /codon_start=1
                     /gene="aph(4)-Ia"
                     /product="aminoglycoside phosphotransferase from E. coli"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MGKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGR
                     GYVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDL
                     PETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHW
                     QTVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAM
                     FGDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVD
                     GNFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRA
                     KE"
     promoter        complement(2010..2353)
                     /label=TEF promoter
                     /note="Ashbya gossypii TEF promoter"
     promoter        2432..3136
                     /gene="S. cerevisiae ADH1"
                     /label=ADH1 promoter
                     /note="promoter for alcohol dehydrogenase 1"
     CDS             3188..4915
                     /codon_start=1
                     /product="rice transport inhibitor response 1 protein, 
                     mutated for use in the AID2 system of inducible degradation
                     (Yesbolatova et al., 2020)"
                     /label=OsTIR1(F74G)
                     /note="F-box protein that mediates auxin-dependent 
                     interaction of a ubiquitin ligase with an auxin-inducible 
                     degron (Tan et al., 2007)"
                     /translation="MTYFPEEVVEHIFSFLPAQRDRNTVSLVCKVWYEIERLSRRGVFV
                     GNCYAVRAGRVAARFPNVRALTVKGKPHGADFNLVPPDWGGYAGPWIEAAARGCHGLEE
                     LRMKRMVVSDESLELLARSFPRFRALVLISCEGFSTDGLAAVASHCKLLRELDLQENEV
                     EDRGPRWLSCFPDSCTSLVSLNFACIKGEVNAGSLERLVSRSPNLRSLRLNRSVSVDTL
                     AKILLRTPNLEDLGTGNLTDDFQTESYFKLTSALEKCKMLRSLSGFWDASPVCLSFIYP
                     LCAQLTGLNLSYAPTLDASDLTKMISRCVKLQRLWVLDCISDKGLQVVASSCKDLQELR
                     VFPSDFYVAGYSAVTEEGLVAVSLGCPKLNSLLYFCHQMTNAALVTVAKNCPNFTRFRL
                     CILEPGKPDVVTSQPLDEGFGAIVRECKGLQRLSISGLLTDKVFMYIGKYAKQLEMLSI
                     AFAGDSDKGMMHVMNGCKNLRKLEIRDSPFGDAALLGNFARYETMRSLWMSSCNVTLKG
                     CQVLASKMPMLNVEVINERDGSNEMEENHGDLPKVEKLYVYRTTAGARDDAPNFVKIL"
     terminator      4922..5169
                     /gene="S. cerevisiae CYC1"
                     /label=CYC1 terminator
                     /note="transcription terminator for CYC1"
     rep_origin      complement(5694..6149)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(5831..5852)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     6043..6062
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     CDS             complement(6126..6413)
                     /codon_start=1
                     /gene="lacZ fragment"
                     /product="LacZ-alpha fragment of beta-galactosidase"
                     /label=lacZ-alpha
                     /translation="MTMITPSSKLTLTKGNKSWYRAPPSNSPYSESYYNSLAVVLQRRD
                     WENPGVTQLNRLAAHPPFASWRNSEEARTDRPSQQLRSLNGEWTRPVAAH"
     primer_bind     6275..6297
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     6289..6306
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     6290..6306
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     6313..6332
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        6313..6331
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     promoter        complement(6370..6388)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(6409..6425)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6409..6425)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(6422..6444)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    6433..6449
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(6457..6463,6464..6481,6482..6487))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6502..6523
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(6640..6657)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(6811..7399)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(6891..6910)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(7570..8361,8362..8430))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     8193..8212
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(8431..8535)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     8603..8621
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
ORIGIN
        1 gcgtttcggt gatgacggtg aaaacctctg acacatgcag ctcccggaga cggtcacagc
       61 ttgtctgtaa gcggatgccg ggagcagaca agcccgtcag ggcgcgtcag cgggtgttgg
      121 cgggtgtcgg ggctggctta actatgcggc atcagagcag attgtactga gagtgcacca
      181 taaattcccg ttttaagagc ttggtgagcg ctaggagtca ctgccaggta tcgtttgaac
      241 acggcattag tcagggaagt cataacacag tcctttcccg caattttctt tttctattac
      301 tcttggcctc ctctagtaca ctctatattt ttttatgcct cggtaatgat tttcattttt
      361 ttttttccac ctagcggatg actctttttt tttcttagcg attggcatta tcacataatg
      421 aattatacat tatataaagt aatgtgattt cttcgaagaa tatactaaaa aatgagcagg
      481 caagataaac gaaggcaaag atgacagagc agaaagccct agtaaagcgt attacaaatg
      541 aaaccaagat tcagattgcg atctctttaa agggtggtcc cctagcgata gagcactcga
      601 tcttcccaga aaaagaggca gaagcagtag cagaacaggc cacacaatcg caagtgatta
      661 acgtccacac aggtataggg tttctggacc atatgataca tgctctggat catcgatgaa
      721 ttcgagctcg ttaaagcctt cgagcgtccc aaaaccttct caagcaaggt tttcagtata
      781 atgttacatg cgtacacgcg tctgtacaga aaaaaaagaa aaatttgaaa tataaataac
      841 gttcttaata ctaacataac tataaaaaaa taaataggga cctagacttc aggttgtcta
      901 actccttcct tttcggttag agcggatgtg gggggagggc gtgaatgtaa gcgtgacata
      961 actaattaca tgactcgaga ttattccttt gccctcggac gagtgctggg gcgtcggttt
     1021 ccactatcgg cgagtacttc tacacagcca tcggtccaga cggccgcgct tctgcgggcg
     1081 atttgtgtac gcccgacagt cccggctccg gatcggacga ttgcgtcgca tcgaccctgc
     1141 gcccaagctg catcatcgaa attgccgtca accaagctct gatagagttg gtcaagacca
     1201 atgcggagca tatacgcccg gagccgcggc gatcctgcaa gctccggatg cctccgctcg
     1261 aagtagcgcg tctgctgctc catacaagcc aaccacggcc tccagaagaa gatgttggcg
     1321 acctcgtatt gggaatcccc gaacatcgcc tcgctccagt caatgaccgc tgttatgcgg
     1381 ccattgtccg tcaggacatt gttggagccg aaatccgcgt gcacgaggtg ccggacttcg
     1441 gggcagtcct cggcccaaag catcagctca tcgagagcct gcgcgacgga cgcactgacg
     1501 gtgtcgtcca tcacagtttg ccagtgatac acatggggat cagcaatcgc gcatatgaaa
     1561 tcacgccatg tagtgtattg accgattcct tgcggtccga atgggccgaa cccgctcgtc
     1621 tggctaagat cggccgcagc gatcgcatcc atggcctccg cgaccggctg cagaacagcg
     1681 ggcagttcgg tttcaggcag gtcttgcaac gtgacaccct gtgcacggcg ggagatgcaa
     1741 taggtcaggc tctcgctgaa ttccccaatg tcaagcactt ccggaatcgg gagcgcggcc
     1801 gatgcaaagt gccgataaac ataacgatct ttgtagaaac catcggcgca gctatttacc
     1861 cgcaggacat atccacgccc tcctacatcg aagctgaaag cacgagattc ttcgccctcc
     1921 gagagctgca tcaggtcgga gacgctgtcg aacttttcga tcagaaactt ctcgacagac
     1981 gtcgcggtga gttcaggctt tttacccatg gttgtttatg ttcggatgtg atgtgagaac
     2041 tgtatcctag caagatttta aaaggaagta tatgaaagaa gaacctcagt ggcaaatcct
     2101 aaccttttat atttctctac aggggcgcgg cgtggggaca attcaacgcg tctgtgaggg
     2161 gagcgtttcc ctgctcgcag gtccgcagcg aggagccgta atttttgctt cgcgccgtgc
     2221 ggccatcaaa atgtatggat gcaaatgatt atacatgggg atgtatgggc taaatgtacg
     2281 ggcgacagtc acatcatgcc cctgagctgc gcacgtcaag actgtcaagg agggtattct
     2341 gggcctccat gtcgctggcc gggtgacccg gcggggacga ggcaagctaa acagatctgg
     2401 cgcgccttaa ttaacccggg gatccgagct catccttttg ttgtttccgg gtgtacaata
     2461 tggacttcct cttttctggc aaccaaaccc atacatcggg attcctataa taccttcgtt
     2521 ggtctcccta acatgtaggt ggcggagggg agatatacaa tagaacagat accagacaag
     2581 acataatggg ctaaacaaga ctacaccaat tacactgcct cattgatggt ggtacataac
     2641 gaactaatac tgtagcccta gacttgatag ccaccatcat atcgaagttt cactaccctt
     2701 tttccatttg ccatctattg aagtaataat aggcgcatgc aacttctttt cttttttttt
     2761 cttttctctc tcccccgttg ttgtctcacc atatccgcaa tgacaaaaaa atgatggaag
     2821 acactaaagg aaaaaattaa cgacaaagac agcaccaaca gatgtcgttg ttccagagct
     2881 gatgaggggt atctcgaagc acacgaaact ttttccttcc ttcattcacg cacactactc
     2941 tctaatgagc aacggtatac ggccttcctt ccagttactt gaatttgaaa taaaaaaaag
     3001 tttgctgtct tgctatcaag tataaataga cctgcaatta ttaatctttt gtttcctcgt
     3061 cattgttctc gttccctttc ttccttgttt ctttttctgc acaatatttc aagctatacc
     3121 aagcatacaa tcaactccaa gctggccgct ctagaagaac ttagtttcga cggattctag
     3181 aactagtatg acttattttc ctgaagaagt tgtagaacac atattttcct ttttacctgc
     3241 tcaaagagat agaaacaccg tctccttggt ctgtaaggtt tggtacgaaa tcgaaagatt
     3301 gtctagaaga ggtgtttttg ttggtaattg ttacgcagtt agagctggta gagttgctgc
     3361 aagattccca aacgttagag cattgactgt taaaggtaaa cctcatggtg ctgatttcaa
     3421 tttggttcca cctgattggg gtggttatgc tggtccatgg attgaagctg cagctagagg
     3481 ttgtcacggt ttggaagaat tgagaatgaa gagaatggtt gtttctgatg aatcattgga
     3541 attgttagca agatcttttc ctagattcag agctttggtt ttgatctcat gtgaaggttt
     3601 ttccaccgat ggtttagcag ctgttgcttc acattgtaag ttgttgagag aattggattt
     3661 gcaagaaaac gaagttgaag atagaggtcc aagatggtta tcttgttttc ctgattcctg
     3721 tacaagtttg gtttctttga acttcgcatg tatcaagggt gaagttaacg ctggtagttt
     3781 ggaaagatta gtttctagat caccaaattt gagatctttg agattgaaca gatccgttag
     3841 tgttgatacc ttggcaaaga tcttgttgag aactcctaat ttggaagatt tgggtactgg
     3901 taacttgaca gatgatttcc aaacagaatc atacttcaag ttgacctccg ctttggaaaa
     3961 gtgtaagatg ttgagatctt tgtcaggttt ttgggatgca agtccagttt gtttgtcttt
     4021 tatatatcct ttgtgtgctc aattgactgg tttgaatttg tcttacgcac caactttgga
     4081 tgctagtgat ttgacaaaga tgatctctag atgtgttaag ttgcaaagat tgtgggtttt
     4141 ggattgtatc tcagataaag gtttgcaagt tgttgcttct tcttgtaagg atttgcaaga
     4201 attgagagtt tttccaagtg atttctatgt tgcaggttac tctgctgtta cagaagaagg
     4261 tttagttgca gtttcattgg gttgtcctaa gttgaactcc ttgttgtact tctgtcatca
     4321 aatgaccaac gcagctttgg ttactgttgc taaaaattgt ccaaacttca ctagattcag
     4381 attgtgtatc ttggaaccag gtaaacctga tgttgttaca tcacaacctt tagatgaagg
     4441 ttttggtgca atcgttagag agtgtaaggg tttgcaaaga ttgtccatca gtggtttgtt
     4501 gacagataag gtttttatgt acatcggtaa atacgctaag caattggaaa tgttgtcaat
     4561 cgcattcgct ggtgactccg ataagggtat gatgcatgtt atgaacggtt gtaagaactt
     4621 aagaaagttg gaaatcagag attctccatt tggtgacgca gctttgttgg gtaatttcgc
     4681 aagatatgaa acaatgagat ctttgtggat gtccagttgt aacgttacct tgaaaggttg
     4741 tcaagtttta gcttcaaaga tgccaatgtt gaacgttgaa gttattaacg aaagagatgg
     4801 ttccaatgaa atggaagaaa accatggtga cttgcctaaa gttgaaaagt tatacgtcta
     4861 cagaacaacc gcaggtgcca gagatgatgc tcctaacttc gtcaagattt tatgactcga
     4921 gtcatgtaat tagttatgtc acgcttacat tcacgccctc cccccacatc cgctctaacc
     4981 gaaaaggaag gagttagaca acctgaagtc taggtcccta tttatttttt tatagttatg
     5041 ttagtattaa gaacgttatt tatatttcaa atttttcttt tttttctgta cagacgcgtg
     5101 tacgcatgta acattatact gaaaaccttg cttgagaagg ttttgggacg ctcgaaggct
     5161 ttaatttgcg gccggtcgac ctgcagcgta cgcagttgtc gaacttggtt tgcaaaggga
     5221 gaaagtagga gatctctctt gcgagatgat cccgcatttt cttgaaagct ttgcagaggc
     5281 tagcagaatt accctccacg ttgattgtct gcgaggcaag aatgatcatc accgtagtga
     5341 gagtgcgttc aaggctcttg cggttgccat aagagaagcc acctcgccca atggtaccaa
     5401 cgatgttccc tccaccaaag gtgttcttat gtagtgacac cgattattta aagctgcagc
     5461 atacgatata tatacatgtg tatatatgta tacctatgaa tgtcagtaag tatgtatacg
     5521 aacagtatga tactgaagat gacaaggtaa tgcatcattc tatacgtgtc attctgaacg
     5581 aggcgcgctt tccttttttc tttttgcttt ttcttttttt ttctcttgaa ctcgacggat
     5641 ctatgcggtg tgaaataccg cacagatgcg taaggagaaa ataccgcatc aggaaattgt
     5701 aagcgttaat attttgttaa aattcgcgtt aaatttttgt taaatcagct cattttttaa
     5761 ccaataggcc gaaatcggca aaatccctta taaatcaaaa gaatagaccg agatagggtt
     5821 gagtgttgtt ccagtttgga acaagagtcc actattaaag aacgtggact ccaacgtcaa
     5881 agggcgaaaa accgtctatc agggcgatgg cccactacgt gaaccatcac cctaatcaag
     5941 ttttttgggg tcgaggtgcc gtaaagcact aaatcggaac cctaaaggga gcccccgatt
     6001 tagagcttga cggggaaagc cggcgaacgt ggcgagaaag gaagggaaga aagcgaaagg
     6061 agcgggcgct agggcgctgg caagtgtagc ggtcacgctg cgcgtaacca ccacacccgc
     6121 cgcgcttaat gcgccgctac agggcgcgtc cattcgccat tcaggctgcg caactgttgg
     6181 gaagggcgat cggtgcgggc ctcttcgcta ttacgccagc tggcgaaagg gggatgtgct
     6241 gcaaggcgat taagttgggt aacgccaggg ttttcccagt cacgacgttg taaaacgacg
     6301 gccagtgaat tgtaatacga ctcactatag ggcgaattgg aggggggggc ccggtaccag
     6361 cttttgttcc ctttagtgag ggttaatttc gagcttggcg taatcatggt catagctgtt
     6421 tcctgtgtga aattgttatc cgctcacaat tccacacaac atacgagccg gaagcataaa
     6481 gtgtaaagcc tggggtgcct aatgagtgag ctaactcaca ttaattgcgt tgcgctcact
     6541 gcccgctttc cagtcgggaa acctgtcgtg ccagctgcat taatgaatcg gccaacgcgc
     6601 ggggagaggc ggtttgcgta ttgggcgctc ttccgcttcc tcgctcactg actcgctgcg
     6661 ctcggtcgtt cggctgcggc gagcggtatc agctcactca aaggcggtaa tacggttatc
     6721 cacagaatca ggggataacg caggaaagaa catgtgagca aaaggccagc aaaaggccag
     6781 gaaccgtaaa aaggccgcgt tgctggcgtt tttccatagg ctccgccccc ctgacgagca
     6841 tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg acaggactat aaagatacca
     6901 ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg
     6961 atacctgtcc gcctttctcc cttcgggaag cgtggcgctt tctcatagct cacgctgtag
     7021 gtatctcagt tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt
     7081 tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc cggtaagaca
     7141 cgacttatcg ccactggcag cagccactgg taacaggatt agcagagcga ggtatgtagg
     7201 cggtgctaca gagttcttga agtggtggcc taactacggc tacactagaa gaacagtatt
     7261 tggtatctgc gctctgctga agccagttac cttcggaaaa agagttggta gctcttgatc
     7321 cggcaaacaa accaccgctg gtagcggtgg tttttttgtt tgcaagcagc agattacgcg
     7381 cagaaaaaaa ggatctcaag aagatccttt gatcttttct acggggtctg acgctcagtg
     7441 gaacgaaaac tcacgttaag ggattttggt catgagatta tcaaaaagga tcttcaccta
     7501 gatcctttta aattaaaaat gaagttttaa atcaatctaa agtatatatg agtaaacttg
     7561 gtctgacagt taccaatgct taatcagtga ggcacctatc tcagcgatct gtctatttcg
     7621 ttcatccata gttgcctgac tccccgtcgt gtagataact acgatacggg agggcttacc
     7681 atctggcccc agtgctgcaa tgataccgcg agacccacgc tcaccggctc cagatttatc
     7741 agcaataaac cagccagccg gaagggccga gcgcagaagt ggtcctgcaa ctttatccgc
     7801 ctccatccag tctattaatt gttgccggga agctagagta agtagttcgc cagttaatag
     7861 tttgcgcaac gttgttgcca ttgctacagg catcgtggtg tcacgctcgt cgtttggtat
     7921 ggcttcattc agctccggtt cccaacgatc aaggcgagtt acatgatccc ccatgttgtg
     7981 caaaaaagcg gttagctcct tcggtcctcc gatcgttgtc agaagtaagt tggccgcagt
     8041 gttatcactc atggttatgg cagcactgca taattctctt actgtcatgc catccgtaag
     8101 atgcttttct gtgactggtg agtactcaac caagtcattc tgagaatagt gtatgcggcg
     8161 accgagttgc tcttgcccgg cgtcaatacg ggataatacc gcgccacata gcagaacttt
     8221 aaaagtgctc atcattggaa aacgttcttc ggggcgaaaa ctctcaagga tcttaccgct
     8281 gttgagatcc agttcgatgt aacccactcg tgcacccaac tgatcttcag catcttttac
     8341 tttcaccagc gtttctgggt gagcaaaaac aggaaggcaa aatgccgcaa aaaagggaat
     8401 aagggcgaca cggaaatgtt gaatactcat actcttcctt tttcaatatt attgaagcat
     8461 ttatcagggt tattgtctca tgagcggata catatttgaa tgtatttaga aaaataaaca
     8521 aataggggtt ccgcgcacat ttccccgaaa agtgccacct gacgtctaag aaaccattat
     8581 tatcatgaca ttaacctata aaaataggcg tatcacgagg ccctttcgtc tcgc
//
