LOCUS       sequence-49610-e         540 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 540)
  AUTHORS   Tasic B, Miyamichi K, Hippenmeyer S, Dani VS, Zeng H, Joo W, Zong H,
            Chen-Tsai Y, Luo L
  TITLE     Extensions of MADM (Mosaic Analysis with Double Markers) in Mice.
  JOURNAL   PLoS One. 2012;7(3):e33332. Epub 2012 Mar 27.
  PUBMED    22479386
REFERENCE   2  (bases 1 to 540)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2012"; volume: "7(3)"; pages: "e33332. Epub 2012 Mar 27"
COMMENT     Sequence Label: pExT
FEATURES             Location/Qualifiers
     source          1..540
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    69..138
                     /label=attB
                     /bound_moiety="phage phi-C31 integrase"
                     /note="attB site for the phi-C31 integrase (Groth et al., 
                     2000)"
     polyA_signal    352..473
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(363..382)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     417..436
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
ORIGIN
        1 ttcctgcagc ccggctgcag gaattcgata tcaagcttat ctataccgtc gacgatgtag
       61 gtcacggtct cgaagccgcg gtgcgggtgc cagggcgtgc ccttgggctc cccgggcgcg
      121 tactccacct cacccatctg gtccatcatg atgaacgggt cgaggtggcg gtagttgatc
      181 ccggcgaacg cgcggcgcac cgggaagccc tcgccctcga aaccgctggg cgcggtggtc
      241 acggtgagca cgggacgtgc gacggcgtcg gcgggtgcgg atacgcgggg cagcgtcagc
      301 gggttctcga cggtcacggc gggcatgtcg acctcgaggg ggggcccgaa ataagataca
      361 ttgatgagtt tggacaaacc acaactagaa tgcagtgaaa aaaatgcttt atttgtgaaa
      421 tttgtgatgc tattgcttta tttgtaacca ttataagctg caataaacaa gttaacaaca
      481 acaattgcat tcattttatg tttcaggttc agggggaggt gtgggaggtt ttttaaagca
//
