LOCUS       sequence-496165-       12306 bp    DNA     circular SYN 05-JAN-2026
DEFINITION  Express HA C-terminal tagged wild-type mouse ADCY3 transiently via 
            transfection or stably via lentivirus-mediated infection in 
            mammalian cells..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12306)
  AUTHORS   Xun Y, Jiang Y, Xu B, Tang M, Ludwig S, Nakamura K, Mukhopadhyay S, 
            Liu C, Beutler B, Zhang Z
  TITLE     GPR45 modulates Galpha(s) at primary cilia of the paraventricular 
            hypothalamus to control food intake.
  JOURNAL   Science. 2025 Jun 5;388(6751):eadp3989. doi: 
            10.1126/science.adp3989. Epub 2025 Jun 5.
  PUBMED    40472089
REFERENCE   2  (bases 1 to 12306)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Jan 5, 2026 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Science."; 
            date: "2025-06-5"; volume: "388(6751):eadp3989. doi"; pages: " 
            10.1126/science.adp3989. Epub 2025 Jun 5"
COMMENT     Sequence Label: pBOB-Adcy3-HA
FEATURES             Location/Qualifiers
     source          1..12306
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(44..63)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        235..614
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        616..814
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     LTR             832..1012
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    1059..1184
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1677..1910
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             2095..2139
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2391..2507
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     enhancer        2530..2833
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        2834..3037
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     3034..3058
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     CDS             6592..6618
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     intron          6707..6936
                     /label=chimeric intron
                     /note="chimera between introns from adenovirus and 
                     immunoglobulin heavy chain genes"
     primer_bind     6919..6938
                     /label=pMT2-F
                     /note="Synthetic intron, forward primer"
     misc_feature    7113..7701
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(7166..7186)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(7584..7595)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             8108..8288
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(8314..8331)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    8320..8544
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      8590..9018
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(8677..8696)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     8887..8908
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(9027..9047)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        9032..9361
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      9212..9347
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     9274..9293
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     promoter        9409..9456
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             9475..9849
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     polyA_signal    9979..10100
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(10016..10035)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     10070..10089
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(10149..10165)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(10149..10165)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(10162..10184)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    10173..10189
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(10197..10203,10204..10221,10222..10227))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    10242..10263
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(10380..10397)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(10551..11139)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(10631..10650)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(11310..12101,12102..12170))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     11933..11952
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(12171..12275)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 gacggatcgg gagatctccc gatcccctat ggtgcactct cagtacaatc tgctctgatg
       61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg
      121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc
      181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt
      241 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata
      301 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc
      361 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc
      421 attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt
      481 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt
      541 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca
      601 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg
      661 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc
      721 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg
      781 gtaggcgtgt acggtgggag gtctatataa gcagcgcgtt ttgcctgtac tgggtctctc
      841 tggttagacc agatctgagc ctgggagctc tctggctaac tagggaaccc actgcttaag
      901 cctcaataaa gcttgccttg agtgcttcaa gtagtgtgtg cccgtctgtt gtgtgactct
      961 ggtaactaga gatccctcag acccttttag tcagtgtgga aaatctctag cagtggcgcc
     1021 cgaacaggga cttgaaagcg aaagggaaac cagaggagct ctctcgacgc aggactcggc
     1081 ttgctgaagc gcgcacggca agaggcgagg ggcggcgact ggtgagtacg ccaaaaattt
     1141 tgactagcgg aggctagaag gagagagatg ggtgcgagag cgtcagtatt aagcggggga
     1201 gaattagatc gcgatgggaa aaaattcggt taaggccagg gggaaagaaa aaatataaat
     1261 taaaacatat agtatgggca agcagggagc tagaacgatt cgcagttaat cctggcctgt
     1321 tagaaacatc agaaggctgt agacaaatac tgggacagct acaaccatcc cttcagacag
     1381 gatcagaaga acttagatca ttatataata cagtagcaac cctctattgt gtgcatcaaa
     1441 ggatagagat aaaagacacc aaggaagctt tagacaagat agaggaagag caaaacaaaa
     1501 gtaagaccac cgcacagcaa gcggccgctg atcttcagac ctggaggagg agatatgagg
     1561 gacaattgga gaagtgaatt atataaatat aaagtagtaa aaattgaacc attaggagta
     1621 gcacccacca aggcaaagag aagagtggtg cagagagaaa aaagagcagt gggaatagga
     1681 gctttgttcc ttgggttctt gggagcagca ggaagcacta tgggcgcagc gtcaatgacg
     1741 ctgacggtac aggccagaca attattgtct ggtatagtgc agcagcagaa caatttgctg
     1801 agggctattg aggcgcaaca gcatctgttg caactcacag tctggggcat caagcagctc
     1861 caggcaagaa tcctggctgt ggaaagatac ctaaaggatc aacagctcct ggggatttgg
     1921 ggttgctctg gaaaactcat ttgcaccact gctgtgcctt ggaatgctag ttggagtaat
     1981 aaatctctgg aacagatttg gaatcacacg acctggatgg agtgggacag agaaattaac
     2041 aattacacaa gcttaataca ctccttaatt gaagaatcgc aaaaccagca agaaaagaat
     2101 gaacaagaat tattggaatt agataaatgg gcaagtttgt ggaattggtt taacataaca
     2161 aattggctgt ggtatataaa attattcata atgatagtag gaggcttggt aggtttaaga
     2221 atagtttttg ctgtactttc tatagtgaat agagttaggc agggatattc accattatcg
     2281 tttcagaccc acctcccaac cccgagggga cccgacaggc ccgaaggaat agaagaagaa
     2341 ggtggagaga gagacagaga cagatccatt cgattagtga acggatcaac ttttaaaaga
     2401 aaagggggga ttggggggta cagtgcaggg gaaagaatag tagacataat agcaacagac
     2461 atacaaacta aagaattaca aaaacaaatt acaaaattca aaattttatc gataagcttg
     2521 ggagttccgc gttacataac ttacggtaaa tggcccgcct ggctgaccgc ccaacgaccc
     2581 ccgcccattg acgtcaataa tgacgtatgt tcccatagta acgccaatag ggactttcca
     2641 ttgacgtcaa tgggtggagt atttacggta aactgcccac ttggcagtac atcaagtgta
     2701 tcatatgcca agtacgcccc ctattgacgt caatgacggt aaatggcccg cctggcatta
     2761 tgcccagtac atgaccttat gggactttcc tacttggcag tacatctacg tattagtcat
     2821 cgctattacc atggtgatgc ggttttggca gtacatcaat gggcgtggat agcggtttga
     2881 ctcacgggga tttccaagtc tccaccccat tgacgtcaat gggagtttgt tttggcacca
     2941 aaatcaacgg gactttccaa aatgtcgtaa caactccgcc ccattgacgc aaatgggcgg
     3001 taggcgtgta cggtgggagg tctatataag cagagctcgt ttagtgaacc gtcagatcgc
     3061 ctggagacgc catccacgct gttttgacct ccatagaaga caccgactga gctccttaag
     3121 gttaactcta gagaattcgg atccgccacc atgccgagga accagggctt ctcggatccc
     3181 gagtactcgg cagagtactc agctgaatac tcggtcagcc tgccctctga ccccgaccgc
     3241 ggggtcggcc ggacccatga aatttctgtg cggaactcgg ggtcctgcct gtgcctgcct
     3301 cgctttatgc ggctgacctt cgtgcctgag tccttggaga acctctacca gacctacttc
     3361 aaaaggcagc gccatgagac cctgctggtg ctggtggtct ttgcggccct ctttgactgc
     3421 tacgtggtag tgatgtgcgc ggtggtcttc tccagcgaca agctggcgcc cctcatggtg
     3481 gcaggcttcg gtctggtgtt ggacatcatc cttttcgtgc tctgtaaaaa ggggctgctc
     3541 ccggaccgag tgagccgcaa agtggtaccc tacctgctgt ggctgctcat ctcggcccag
     3601 atcttctcct acctgggcct gaacttttca cgggcccacg cagccagtga cactgtgggt
     3661 tggcaggctt tctttgtctt ctccttcttc ataacgctgc ccctcagcct cagccccatc
     3721 gtgatcatct ctgtggtctc ctgtgttgtg cacacgcttg tgttgggggt cactgtggcc
     3781 cagcagcagc aggatgagct ggaagggatg cagctgctga gggagatcct ggccaacgtc
     3841 ttcctctacc tgtgtgctat catcgtgggc atcatgtcct actacatggc agaccgcaag
     3901 caccgcaagg ccttcctgga ggcccgccag tcactggagg tgaagatgaa tctggaggag
     3961 cagagccagc agcaggaaaa ccttatgctt tccatcctgc ccaagcacgt ggctgacgag
     4021 atgctgaagg acatgaagaa ggacgagagc cagaaggacc agcagcagtt caataccatg
     4081 tacatgtacc ggcacgagaa tgtcagcatc ctgtttgcag atattgtggg ctttacccag
     4141 ctgtcctctg cttgcagtgc ccaggagctc gtgaagttac tcaacgagct cttcgcccgc
     4201 tttgacaagc tggcagccaa ataccaccag ctgaggatca agatcctagg cgactgttac
     4261 tactgcatct gcggcttgcc tgactaccgg gaggaccatg ccgtgtgctc catccttatg
     4321 gggcttgcca tggtagaggc catctcgtac gtgcgggaga agaccaagac tggagtggac
     4381 atgcgtgtgg gagtgcacac aggcactgtc ctaggcggtg tcctgggcca gaagcgctgg
     4441 cagtatgatg tatggtctac tgatgtcacc gtggcaaaca agatggaggc tggtggcatc
     4501 ccagggcgcg tgcacatttc ccagagcacc atggactgcc tgaaggggga gtttgatgtg
     4561 gagcctggtg atgggggcag tcgctgcgac tacctagatg agaagggcat cgaaacctac
     4621 ctcatcattg cctccaagcc agaggtgaag aaaacagccc aaaatggcct caacggctcg
     4681 gccgtgccaa acggagcgcc ggcatcctcc aaacccagct cccctgccct catcgagacc
     4741 aaggagccca atggaagtgc ccatgccagc ggctccacat cagaggaggc tgaagaacag
     4801 gaggcccagg ctgacaaccc ctcgttcccc aacccccgcc gcaggctgcg cctccaggac
     4861 ctggcggacc gtgtggtgga cgcctctgag gatgagcacg aactgaacca gctgcttaat
     4921 gaggccctgc tggagcggga gtccgcccag gtggtaaaga agagaaacac attcctcctg
     4981 accatgaggt tcatggaccc agagatggaa acacgctact cggtggagaa ggagaagcag
     5041 agcggggctg ccttcagctg ctcctgtgtg gtcctcttct gcacggccat ggtggagata
     5101 ctcattgacc cctggctgat gacaaactat gtgacctttg tggttgggga ggttctgctc
     5161 ctgatcctga ccatctgctc gatggctgcc atctttccca ggtcatttcc caagaagctt
     5221 gtggccttct catcttggat tgaccggacc cgctgggcaa ggaacacctg ggccatgtta
     5281 gccatcttca ttctggttat ggccaatgtt gtggacatgc tcagctgtct ccagtactac
     5341 atgggacctt acaacatgac agctgggatg gagctggacg gcggctgcat ggagaacccc
     5401 aagtactaca actacgtagc tgtgctgtcg ctcatcgcca ccatcatgct ggtgcaggtc
     5461 agccacatgg tgaagctgac gctcatgctg ctcgtcacag gcgccgtgac tgccctcaac
     5521 ctgtacgcct ggtgtcctgt ctttgatgaa tacgaccaca agcgctttca ggaaaaggac
     5581 tctcctatgg tggccttaga gaagatgcag gtacttgcca cccctgggct caatggcact
     5641 gacagcaggc tgcccctggt gccttccaag tactccatga ctgtgatgat gttcgtcatg
     5701 atgctgagct tttactactt ctcgcgccac gtggaaaaac tggcccgaac actgttcttg
     5761 tggaagattg aggtccatga ccagaaagaa cgtgtctacg agatgcgccg atggaacgag
     5821 gccttggtca ccaacatgtt gcctgagcat gttgcacgcc attttctggg gtccaagaag
     5881 agagatgagg agctgtacag ccagtcttat gacgagattg gagtcatgtt tgcctccttg
     5941 cccaactttg ctgacttcta cactgaggag agcatcaaca acggcggcat cgagtgtcta
     6001 cgcttcctca atgagatcat ctctgatttt gactctctcc tggacaatcc caaattccgg
     6061 gtcatcacca agatcaaaac tattggcagc acctatatgg cagcttcagg agtcacacca
     6121 gatgtcaaca ccaatggctt tacaagctcc agcaaggagg agaagtcaga caaggagcgc
     6181 tggcagcacc tggctgacct ggctgacttt gcattagcca tgaaggacac gctcacaaac
     6241 atcaacaacc agtcattcaa caacttcatg ttgcgcatag gcatgaacaa aggaggggtt
     6301 ctggctggcg tcattggagc ccggaaacca cactatgaca tctggggcaa tacggtcaat
     6361 gtggccagca ggatggaatc cacaggagtc atgggcaaca tccaggtggt agaagagacg
     6421 caggtcatcc ttcgagagta cggcttccgc tttgtgaggc gaggtcccat ctttgtgaaa
     6481 ggcaaagggg agcttctgac ctttttcttg aaggggcggg acaggccagc tgccttcccc
     6541 aatggctcct ctgttacact gccccaccaa gtggtggaca accccctcga gtacccatac
     6601 gatgttccag attacgctta atcgagccat ggaagcttga tatctaactg actgaaccgg
     6661 tggtaccgag gaattaattc gctgtctgcg agggccagct gttggggtga gtactccctc
     6721 tcaaaagcgg gcatgacttc tgcgctaaga ttgtcagttt ccaaaaacga ggaggatttg
     6781 atattcacct ggcccgcggt gatgcctttg agggtggccg cgtccatctg gtcagaaaag
     6841 acaatctttt tgttgtcaag cttgaggtgt ggcaggcttg agatctggcc atacacttga
     6901 gtgacaatga catccacttt gcctttctct ccacaggtgt ccactcccag gtccaactgc
     6961 aggtcgagca tgcatctagg gcggccaatt ccgccgatcc tggaaaaaca tggagcaatc
     7021 acaagtagca atacagcagc taccaatgct gattgtgcct ggctagaagc acaagaggag
     7081 gaggaggtgg gttttccagt cacacctcag acaatcaacc tctggattac aaaatttgtg
     7141 aaagattgac tggtattctt aactatgttg ctccttttac gctatgtgga tacgctgctt
     7201 taatgccttt gtatcatgct attgcttccc gtatggcttt cattttctcc tccttgtata
     7261 aatcctggtt gctgtctctt tatgaggagt tgtggcccgt tgtcaggcaa cgtggcgtgg
     7321 tgtgcactgt gtttgctgac gcaaccccca ctggttgggg cattgccacc acctgtcagc
     7381 tcctttccgg gactttcgct ttccccctcc ctattgccac ggcggaactc atcgccgcct
     7441 gccttgcccg ctgctggaca ggggctcggc tgttgggcac tgacaattcc gtggtgttgt
     7501 cggggaagct gacgtccttt ccatggctgc tcgcctgtgt tgccacctgg attctgcgcg
     7561 ggacgtcctt ctgctacgtc ccttcggccc tcaatccagc ggaccttcct tcccgcggcc
     7621 tgctgccggc tctgcggcct cttccgcgtc ttcgccttcg ccctcagacg agtcggatct
     7681 ccctttgggc cgcctccccg cctggaattc gagctcggta cctttaagac caatgactta
     7741 caaggcagct gtagatctta gccacttttt aaaagaaaag gggggactgg aagggctaat
     7801 tcactcccaa cgaagacaag atatccttga tctgtggatc taccacacac aaggctactt
     7861 ccctgattgg cagaactaca caccagggcc agggatcaga tatccactga cctttggatg
     7921 gtgctacaag ctagtaccag ttgagcaaga gaaggtagaa gaagccaatg aaggagagaa
     7981 cacccgcttg ttacaccctg tgagcctgca tgggatggat gacccggaga gagaagtatt
     8041 agagtggagg tttgacagcc gcctagcatt tcatcacatg gcccgagagc tgcatccgga
     8101 ctgtactggg tctctctggt tagaccagat ctgagcctgg gagctctctg gctaactagg
     8161 gaacccactg cttaagcctc aataaagctt gccttgagtg cttcaagtag tgtgtgcccg
     8221 tctgttgtgt gactctggta actagagatc cctcagaccc ttttagtcag tgtggaaaat
     8281 ctctagcagg gccgctttaa acccgctgat cagcctcgac tgtgccttct agttgccagc
     8341 catctgttgt ttgcccctcc cccgtgcctt ccttgaccct ggaaggtgcc actcccactg
     8401 tcctttccta ataaaatgag gaaattgcat cgcattgtct gagtaggtgt cattctattc
     8461 tggggggtgg ggtggggcag gacagcaagg gggaggattg ggaagacaat agcaggcatg
     8521 ctggggatgc ggtgggctct atggcttctg aggcggaaag aaccagctgg ggctctaggg
     8581 ggtatcccca cgcgccctgt agcggcgcat taagcgcggc gggtgtggtg gttacgcgca
     8641 gcgtgaccgc tacacttgcc agcgccctag cgcccgctcc tttcgctttc ttcccttcct
     8701 ttctcgccac gttcgccggc tttccccgtc aagctctaaa tcgggggctc cctttagggt
     8761 tccgatttag tgctttacgg cacctcgacc ccaaaaaact tgattagggt gatggttcac
     8821 gtagtgggcc atcgccctga tagacggttt ttcgcccttt gacgttggag tccacgttct
     8881 ttaatagtgg actcttgttc caaactggaa caacactcaa ccctatctcg gtctattctt
     8941 ttgatttata agggattttg ccgatttcgg cctattggtt aaaaaatgag ctgatttaac
     9001 aaaaatttaa cgcgaattaa ttctgtggaa tgtgtgtcag ttagggtgtg gaaagtcccc
     9061 aggctcccca gcaggcagaa gtatgcaaag catgcatctc aattagtcag caaccaggtg
     9121 tggaaagtcc ccaggctccc cagcaggcag aagtatgcaa agcatgcatc tcaattagtc
     9181 agcaaccata gtcccgcccc taactccgcc catcccgccc ctaactccgc ccagttccgc
     9241 ccattctccg ccccatggct gactaatttt ttttatttat gcagaggccg aggccgcctc
     9301 tgcctctgag ctattccaga agtagtgagg aggctttttt ggaggcctag gcttttgcaa
     9361 aaagctcccg ggagcttgta tatccatttt cggatctgat cagcacgtgt tgacaattaa
     9421 tcatcggcat agtatatcgg catagtataa tacgacaagg tgaggaacta aaccatggcc
     9481 aagttgacca gtgccgttcc ggtgctcacc gcgcgcgacg tcgccggagc ggtcgagttc
     9541 tggaccgacc ggctcgggtt ctcccgggac ttcgtggagg acgacttcgc cggtgtggtc
     9601 cgggacgacg tgaccctgtt catcagcgcg gtccaggacc aggtggtgcc ggacaacacc
     9661 ctggcctggg tgtgggtgcg cggcctggac gagctgtacg ccgagtggtc ggaggtcgtg
     9721 tccacgaact tccgggacgc ctccgggccg gccatgaccg agatcggcga gcagccgtgg
     9781 gggcgggagt tcgccctgcg cgacccggcc ggcaactgcg tgcacttcgt ggccgaggag
     9841 caggactgac acgtgctacg agatttcgat tccaccgccg ccttctatga aaggttgggc
     9901 ttcggaatcg ttttccggga cgccggctgg atgatcctcc agcgcgggga tctcatgctg
     9961 gagttcttcg cccaccccaa cttgtttatt gcagcttata atggttacaa ataaagcaat
    10021 agcatcacaa atttcacaaa taaagcattt ttttcactgc attctagttg tggtttgtcc
    10081 aaactcatca atgtatctta tcatgtctgt ataccgtcga cctctagcta gagcttggcg
    10141 taatcatggt catagctgtt tcctgtgtga aattgttatc cgctcacaat tccacacaac
    10201 atacgagccg gaagcataaa gtgtaaagcc tggggtgcct aatgagtgag ctaactcaca
    10261 ttaattgcgt tgcgctcact gcccgctttc cagtcgggaa acctgtcgtg ccagctgcat
    10321 taatgaatcg gccaacgcgc ggggagaggc ggtttgcgta ttgggcgctc ttccgcttcc
    10381 tcgctcactg actcgctgcg ctcggtcgtt cggctgcggc gagcggtatc agctcactca
    10441 aaggcggtaa tacggttatc cacagaatca ggggataacg caggaaagaa catgtgagca
    10501 aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt tttccatagg
    10561 ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg
    10621 acaggactat aaagatacca ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt
    10681 ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc cttcgggaag cgtggcgctt
    10741 tctcatagct cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc caagctgggc
    10801 tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt
    10861 gagtccaacc cggtaagaca cgacttatcg ccactggcag cagccactgg taacaggatt
    10921 agcagagcga ggtatgtagg cggtgctaca gagttcttga agtggtggcc taactacggc
    10981 tacactagaa gaacagtatt tggtatctgc gctctgctga agccagttac cttcggaaaa
    11041 agagttggta gctcttgatc cggcaaacaa accaccgctg gtagcggtgg tttttttgtt
    11101 tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag aagatccttt gatcttttct
    11161 acggggtctg acgctcagtg gaacgaaaac tcacgttaag ggattttggt catgagatta
    11221 tcaaaaagga tcttcaccta gatcctttta aattaaaaat gaagttttaa atcaatctaa
    11281 agtatatatg agtaaacttg gtctgacagt taccaatgct taatcagtga ggcacctatc
    11341 tcagcgatct gtctatttcg ttcatccata gttgcctgac tccccgtcgt gtagataact
    11401 acgatacggg agggcttacc atctggcccc agtgctgcaa tgataccgcg agacccacgc
    11461 tcaccggctc cagatttatc agcaataaac cagccagccg gaagggccga gcgcagaagt
    11521 ggtcctgcaa ctttatccgc ctccatccag tctattaatt gttgccggga agctagagta
    11581 agtagttcgc cagttaatag tttgcgcaac gttgttgcca ttgctacagg catcgtggtg
    11641 tcacgctcgt cgtttggtat ggcttcattc agctccggtt cccaacgatc aaggcgagtt
    11701 acatgatccc ccatgttgtg caaaaaagcg gttagctcct tcggtcctcc gatcgttgtc
    11761 agaagtaagt tggccgcagt gttatcactc atggttatgg cagcactgca taattctctt
    11821 actgtcatgc catccgtaag atgcttttct gtgactggtg agtactcaac caagtcattc
    11881 tgagaatagt gtatgcggcg accgagttgc tcttgcccgg cgtcaatacg ggataatacc
    11941 gcgccacata gcagaacttt aaaagtgctc atcattggaa aacgttcttc ggggcgaaaa
    12001 ctctcaagga tcttaccgct gttgagatcc agttcgatgt aacccactcg tgcacccaac
    12061 tgatcttcag catcttttac tttcaccagc gtttctgggt gagcaaaaac aggaaggcaa
    12121 aatgccgcaa aaaagggaat aagggcgaca cggaaatgtt gaatactcat actcttcctt
    12181 tttcaatatt attgaagcat ttatcagggt tattgtctca tgagcggata catatttgaa
    12241 tgtatttaga aaaataaaca aataggggtt ccgcgcacat ttccccgaaa agtgccacct
    12301 gacgtc
//
