LOCUS       sequence-496167-        3803 bp    DNA     circular SYN 05-JAN-2026
DEFINITION  Express the human eIF2 alpha subunit in insect cells or clone it in 
            other vectors.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3803)
  AUTHORS   Diaz-Lopez I, Gordiyenko Y, Zuber PK, Li X, Ramakrishnan V
  TITLE     Purification and characterization of recombinant human translation 
            initiation factor eIF3.
  JOURNAL   Protein Sci. 2026 Jan;35(1):e70388. doi: 10.1002/pro.70388.
  PUBMED    41432235
REFERENCE   2  (bases 1 to 3803)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Jan 5, 2026 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Protein 
            Sci."; date: "2026-01"; volume: "35(1):e70388. doi"; pages: " 
            10.1002/pro.70388"
COMMENT     Sequence Label: pACEBac_eIF2_alpha
FEATURES             Location/Qualifiers
     source          1..3803
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    complement(1..34)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     promoter        111..202
                     /gene="polh from Autographa californica"
                     /label=polyhedrin promoter
                     /note="promoter for the baculovirus polyhedrin gene"
     primer_bind     127..144
                     /label=Polyhedrin-F
                     /note="Polyhedrin promoter, forward primer"
     polyA_signal    1360..1494
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(1397..1416)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     1451..1470
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     mobile_element  1615..1780
                     /mobile_element_type="transposon:Tn7"
                     /label=Tn7L
                     /note="mini-Tn7 element (left end of the Tn7 transposon)"
     rep_origin      1970..2558
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2459..2478
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     mobile_element  2693..2916
                     /mobile_element_type="transposon:Tn7"
                     /label=Tn7R
                     /note="mini-Tn7 element (right end of the Tn7 transposon)"
     CDS             complement(2983..3516)
                     /codon_start=1
                     /gene="aacC1"
                     /product="gentamycin acetyltransferase"
                     /label=GmR
                     /note="confers resistance to gentamycin"
                     /translation="MLRSSNDVTQQGSRPKTKLGGSSMGIIRTCRLGPDQVKSMRAALD
                     LFGREFGDVATYSQHQPDSDYLGNLLRSKTFIALAAFDQEAVVGALAAYVLPKFEQPRS
                     EIYIYDLAVSGEHRRQGIATALINLLKHEANALGAYVIYVQADYGDDPAVALYTKLGIR
                     EEVMHFDIDPSTAT"
     promoter        complement(join(3705..3710,3711..3727,3728..3733))
                     /gene="intI1 (promoter lies within the coding sequence)"
                     /label=Pc promoter
                     /note="class 1 integron promoter"
ORIGIN
        1 ataacttcgt atagcataca ttatacgaag ttatctgtaa ctataacggt cctaaggtag
       61 cgagtttaaa cactagtatc gattcgcgac ctactccgga atattaatag atcatggaga
      121 taattaaaat gataaccatc tcgcaaataa ataagtattt tactgttttc gtaacagttt
      181 tgtaataaaa aaacctataa atattccgga ttattcatac cgtcccacca tcgggcgcgg
      241 atccctcgag tcaacatgcc gggtctaagt tgtagatttt atcaacacaa atttcctgag
      301 gtggaagatg tagtgatggt gaatgtcaga tccattgctg aaatgggggc ttatgtcagc
      361 ttgctggaat acaacaacat tgaaggcatg attcttctta gtgaattatc cagaaggcgt
      421 atccgttcta tcaacaaact catccgaatt ggcaggaatg agtgtgtggt tgtcattagg
      481 gtggacaaag aaaaaggata tattgatttg tcaaaaagaa gagtttctcc agaggaagca
      541 atcaaatgtg aagacaaatt cacaaaatcc aaaactgttt atagcattct tcgtcatgtt
      601 gctgaggtgt tagaatacac caaggatgag cagctggaaa gcctattcca gaggactgcc
      661 tgggtctttg atgacaagta caagagacct ggatatggtg cctatgatgc atttaagcat
      721 gcagtctcag acccatctat tttggatagt ttagatttga atgaagatga acgggaagta
      781 ctcattaata atattaatag gcgcttgacc ccacaggctg tcaaaattcg agcagatatt
      841 gaagtggctt gttatggtta tgaaggcatt gatgctgtaa aagaagccct aagagcaggt
      901 ttgaattgtt ctacagaaaa catgcccatt aagattaatc taatagctcc tcctcggtat
      961 gtaatgacta cgacaaccct ggagagaaca gaaggccttt ctgtcctcag tcaagctatg
     1021 gctgttatca aagagaagat tgaggaaaag aggggtgtgt tcaatgttca aatggagccc
     1081 aaagtggtca cagatacaga tgagactgaa cttgcgaggc agatggagag gcttgaaaga
     1141 gaaaatgccg aagtggatgg agatgatgat gcagaagaaa tggaagccaa agctgaagat
     1201 taaggtacct ctagagcctg cagtctcgac aagcttgtcg agaagtacta gaggatcata
     1261 atcagccata ccacatttgt agaggtttta cttgctttaa aaaacctccc acacctcccc
     1321 ctgaacctga aacataaaat gaatgcaatt gttgttgtta acttgtttat tgcagcttat
     1381 aatggttaca aataaagcaa tagcatcaca aatttcacaa ataaagcatt tttttcactg
     1441 cattctagtt gtggtttgtc caaactcatc aatgtatctt atcatgtctg gatctgatca
     1501 ctgcttgagc ctagaagatc cggctgctaa caaagcccga aaggaagctg agttggctgc
     1561 tgccaccgct gagcaataac tatcataacc cctagggtat acccatctaa ttggaaccag
     1621 ataagtgaaa tctagttcca aactattttg tcatttttaa ttttcgtatt agcttacgac
     1681 gctacaccca gttcccatct attttgtcac tcttccctaa ataatcctta aaaactccat
     1741 ttccacccct cccagttccc aactattttg tccgcccaca accggttgac ttgggtcaac
     1801 tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta
     1861 aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt
     1921 tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt
     1981 tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt
     2041 gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc
     2101 agataccaaa tactgttctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg
     2161 tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg
     2221 ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt
     2281 cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac
     2341 tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg
     2401 acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg
     2461 gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat
     2521 ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt
     2581 tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg
     2641 attgacttgg gtcgctcttc ctgtggatgc gcagatgccc tgcgtaagcg ggtgtgggcg
     2701 gacaataaag tcttaaactg aacaaaatag atctaaacta tgacaataaa gtcttaaact
     2761 agacagaata gttgtaaact gaaatcagtc cagttatgct gtgaaaaagc atactggact
     2821 tttgttatgg ctaaagcaaa ctcttcattt tctgaagtgc aaattgcccg tcgtattaaa
     2881 gaggggcgtg gccaagggca tgtaaagact atattcgcgg cgttgtgaca atttaccgaa
     2941 caactccgcg gccgggaagc cgatctcggc ttgaacgaat tgttaggtgg cggtacttgg
     3001 gtcgatatca aagtgcatca cttcttcccg tatgcccaac tttgtataga gagccactgc
     3061 gggatcgtca ccgtaatctg cttgcacgta gatcacataa gcaccaagcg cgttggcctc
     3121 atgcttgagg agattgatga gcgcggtggc aatgccctgc ctccggtgct cgccggagac
     3181 tgcgagatca tagatataga tctcactacg cggctgctca aacttgggca gaacgtaagc
     3241 cgcgagagcg ccaacaaccg cttcttggtc gaaggcagca agcgcgatga atgtcttact
     3301 acggagcaag ttcccgaggt aatcggagtc cggctgatgt tgggagtagg tggctacgtc
     3361 tccgaactca cgaccgaaaa gatcaagagc agcccgcatg gatttgactt ggtcagggcc
     3421 gagcctacat gtgcgaatga tgcccatact tgagccacct aactttgttt tagggcgact
     3481 gccctgctgc gtaacatcgt tgctgctgcg taacatcgtt gctgctccat aacatcaaac
     3541 atcgacccac ggcgtaacgc gcttgctgct tggatgcccg aggcatagac tgtacaaaaa
     3601 aacagtcata acaagccatg aaaaccgcca ctgcgccgtt accaccgctg cgttcggtca
     3661 aggttctgga ccagttgcgt gagcgcatac gctacttgca ttacagttta cgaaccgaac
     3721 aggcttatgt caactgggtt cgtgccttca tccgtttcca cggtgtgcgt cacccggcaa
     3781 ccttgggcag cagcgaagtc gcc
//
