LOCUS       sequence-496168-        3857 bp    DNA     circular SYN 05-JAN-2026
DEFINITION  Express the human eIF2 beta subunit in insect cells or clone it in 
            other vectors.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3857)
  AUTHORS   Diaz-Lopez I, Gordiyenko Y, Zuber PK, Li X, Ramakrishnan V
  TITLE     Purification and characterization of recombinant human translation 
            initiation factor eIF3.
  JOURNAL   Protein Sci. 2026 Jan;35(1):e70388. doi: 10.1002/pro.70388.
  PUBMED    41432235
REFERENCE   2  (bases 1 to 3857)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Jan 5, 2026 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Protein 
            Sci."; date: "2026-01"; volume: "35(1):e70388. doi"; pages: " 
            10.1002/pro.70388"
COMMENT     Sequence Label: pACEBac_eIF2_beta
FEATURES             Location/Qualifiers
     source          1..3857
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    complement(1..34)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     promoter        111..202
                     /gene="polh from Autographa californica"
                     /label=polyhedrin promoter
                     /note="promoter for the baculovirus polyhedrin gene"
     primer_bind     127..144
                     /label=Polyhedrin-F
                     /note="Polyhedrin promoter, forward primer"
     polyA_signal    1414..1548
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(1451..1470)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     1505..1524
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     mobile_element  1669..1834
                     /mobile_element_type="transposon:Tn7"
                     /label=Tn7L
                     /note="mini-Tn7 element (left end of the Tn7 transposon)"
     rep_origin      2024..2612
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2513..2532
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     mobile_element  2747..2970
                     /mobile_element_type="transposon:Tn7"
                     /label=Tn7R
                     /note="mini-Tn7 element (right end of the Tn7 transposon)"
     CDS             complement(3037..3570)
                     /codon_start=1
                     /gene="aacC1"
                     /product="gentamycin acetyltransferase"
                     /label=GmR
                     /note="confers resistance to gentamycin"
                     /translation="MLRSSNDVTQQGSRPKTKLGGSSMGIIRTCRLGPDQVKSMRAALD
                     LFGREFGDVATYSQHQPDSDYLGNLLRSKTFIALAAFDQEAVVGALAAYVLPKFEQPRS
                     EIYIYDLAVSGEHRRQGIATALINLLKHEANALGAYVIYVQADYGDDPAVALYTKLGIR
                     EEVMHFDIDPSTAT"
     promoter        complement(join(3759..3764,3765..3781,3782..3787))
                     /gene="intI1 (promoter lies within the coding sequence)"
                     /label=Pc promoter
                     /note="class 1 integron promoter"
ORIGIN
        1 ataacttcgt atagcataca ttatacgaag ttatctgtaa ctataacggt cctaaggtag
       61 cgagtttaaa cactagtatc gattcgcgac ctactccgga atattaatag atcatggaga
      121 taattaaaat gataaccatc tcgcaaataa ataagtattt tactgttttc gtaacagttt
      181 tgtaataaaa aaacctataa atattccgga ttattcatac cgtcccacca tcgggcgcgg
      241 atccctcgag tcaacatgtc tggggacgag atgatttttg atcctactat gagcaagaag
      301 aaaaagaaga agaagaagcc ttttatgtta gatgaggaag gggataccca aacagaggaa
      361 acccagcctt cagaaacaaa agaagtggag ccagagccaa ctgaggacaa ggatttggaa
      421 gctgatgaag aggacactag gaaaaaagat gcttctgatg atctagatga cttgaacttc
      481 tttaatcaaa agaaaaagaa gaaaaaaact aaaaagatat ttgatattga tgaagctgaa
      541 gaaggtgtaa aggatcttaa gattgaaagt gatgttcaag aaccaactga accagaggat
      601 gaccttgaca ttatgcttgg caataaaaag aagaaaaaga agaatgttaa gttcccagat
      661 gaggatgaaa tactagagaa agatgaagct ctagaagatg aagacaacaa aaaagatgat
      721 ggtatctcat tcagtaatca gacaggccct gcttgggcag gctcagaaag agactacaca
      781 tacgaggagc tgctgaatcg agtgttcaac atcatgaggg aaaagaatcc agatatggtt
      841 gctggggaga aaaggaaatt tgtcatgaaa cctccacaag tcgtccgagt aggaaccaag
      901 aaaacttctt ttgtcaactt tacagatatc tgtaaactat tacatcgtca gcccaaacat
      961 ctccttgcat ttttgttggc tgaattgggt acaagtggtt ctatagatgg taataaccaa
     1021 cttgtaatca aaggaagatt ccaacagaaa cagatagaaa atgtcttgag aagatatatc
     1081 aaggaatatg tcacttgtca cacatgccga tcaccggaca caatcctgca gaaggacaca
     1141 cgactctatt tcctacagtg cgaaacttgt cattctagat gttctgttgc cagtatcaaa
     1201 accggcttcc aggctgtcac gggcaagcga gcacagctcc gtgccaaagc taactaaggt
     1261 acctctagag cctgcagtct cgacaagctt gtcgagaagt actagaggat cataatcagc
     1321 cataccacat ttgtagaggt tttacttgct ttaaaaaacc tcccacacct ccccctgaac
     1381 ctgaaacata aaatgaatgc aattgttgtt gttaacttgt ttattgcagc ttataatggt
     1441 tacaaataaa gcaatagcat cacaaatttc acaaataaag catttttttc actgcattct
     1501 agttgtggtt tgtccaaact catcaatgta tcttatcatg tctggatctg atcactgctt
     1561 gagcctagaa gatccggctg ctaacaaagc ccgaaaggaa gctgagttgg ctgctgccac
     1621 cgctgagcaa taactatcat aacccctagg gtatacccat ctaattggaa ccagataagt
     1681 gaaatctagt tccaaactat tttgtcattt ttaattttcg tattagctta cgacgctaca
     1741 cccagttccc atctattttg tcactcttcc ctaaataatc cttaaaaact ccatttccac
     1801 ccctcccagt tcccaactat tttgtccgcc cacaaccggt tgacttgggt caactgtcag
     1861 accaagttta ctcatatata ctttagattg atttaaaact tcatttttaa tttaaaagga
     1921 tctaggtgaa gatccttttt gataatctca tgaccaaaat cccttaacgt gagttttcgt
     1981 tccactgagc gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat cctttttttc
     2041 tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct accagcggtg gtttgtttgc
     2101 cggatcaaga gctaccaact ctttttccga aggtaactgg cttcagcaga gcgcagatac
     2161 caaatactgt tcttctagtg tagccgtagt taggccacca cttcaagaac tctgtagcac
     2221 cgcctacata cctcgctctg ctaatcctgt taccagtggc tgctgccagt ggcgataagt
     2281 cgtgtcttac cgggttggac tcaagacgat agttaccgga taaggcgcag cggtcgggct
     2341 gaacgggggg ttcgtgcaca cagcccagct tggagcgaac gacctacacc gaactgagat
     2401 acctacagcg tgagctatga gaaagcgcca cgcttcccga agggagaaag gcggacaggt
     2461 atccggtaag cggcagggtc ggaacaggag agcgcacgag ggagcttcca gggggaaacg
     2521 cctggtatct ttatagtcct gtcgggtttc gccacctctg acttgagcgt cgatttttgt
     2581 gatgctcgtc aggggggcgg agcctatgga aaaacgccag caacgcggcc tttttacggt
     2641 tcctggcctt ttgctggcct tttgctcaca tgttctttcc tgcgttatcc cctgattgac
     2701 ttgggtcgct cttcctgtgg atgcgcagat gccctgcgta agcgggtgtg ggcggacaat
     2761 aaagtcttaa actgaacaaa atagatctaa actatgacaa taaagtctta aactagacag
     2821 aatagttgta aactgaaatc agtccagtta tgctgtgaaa aagcatactg gacttttgtt
     2881 atggctaaag caaactcttc attttctgaa gtgcaaattg cccgtcgtat taaagagggg
     2941 cgtggccaag ggcatgtaaa gactatattc gcggcgttgt gacaatttac cgaacaactc
     3001 cgcggccggg aagccgatct cggcttgaac gaattgttag gtggcggtac ttgggtcgat
     3061 atcaaagtgc atcacttctt cccgtatgcc caactttgta tagagagcca ctgcgggatc
     3121 gtcaccgtaa tctgcttgca cgtagatcac ataagcacca agcgcgttgg cctcatgctt
     3181 gaggagattg atgagcgcgg tggcaatgcc ctgcctccgg tgctcgccgg agactgcgag
     3241 atcatagata tagatctcac tacgcggctg ctcaaacttg ggcagaacgt aagccgcgag
     3301 agcgccaaca accgcttctt ggtcgaaggc agcaagcgcg atgaatgtct tactacggag
     3361 caagttcccg aggtaatcgg agtccggctg atgttgggag taggtggcta cgtctccgaa
     3421 ctcacgaccg aaaagatcaa gagcagcccg catggatttg acttggtcag ggccgagcct
     3481 acatgtgcga atgatgccca tacttgagcc acctaacttt gttttagggc gactgccctg
     3541 ctgcgtaaca tcgttgctgc tgcgtaacat cgttgctgct ccataacatc aaacatcgac
     3601 ccacggcgta acgcgcttgc tgcttggatg cccgaggcat agactgtaca aaaaaacagt
     3661 cataacaagc catgaaaacc gccactgcgc cgttaccacc gctgcgttcg gtcaaggttc
     3721 tggaccagtt gcgtgagcgc atacgctact tgcattacag tttacgaacc gaacaggctt
     3781 atgtcaactg ggttcgtgcc ttcatccgtt tccacggtgt gcgtcacccg gcaaccttgg
     3841 gcagcagcga agtcgcc
//
