LOCUS       sequence-496169-        4385 bp    DNA     circular SYN 05-JAN-2026
DEFINITION  Express the human eIF2 gamma subunit in insect cells or clone it in 
            other vectors.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4385)
  AUTHORS   Diaz-Lopez I, Gordiyenko Y, Zuber PK, Li X, Ramakrishnan V
  TITLE     Purification and characterization of recombinant human translation 
            initiation factor eIF3.
  JOURNAL   Protein Sci. 2026 Jan;35(1):e70388. doi: 10.1002/pro.70388.
  PUBMED    41432235
REFERENCE   2  (bases 1 to 4385)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Jan 5, 2026 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Protein 
            Sci."; date: "2026-01"; volume: "35(1):e70388. doi"; pages: " 
            10.1002/pro.70388"
COMMENT     Sequence Label: pACEBac_eIF2_gamma
FEATURES             Location/Qualifiers
     source          1..4385
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    complement(1..34)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     promoter        111..202
                     /gene="polh from Autographa californica"
                     /label=polyhedrin promoter
                     /note="promoter for the baculovirus polyhedrin gene"
     primer_bind     127..144
                     /label=Polyhedrin-F
                     /note="Polyhedrin promoter, forward primer"
     CDS             1675..1698
                     /codon_start=1
                     /product="recognition and cleavage site for human 
                     rhinovirus 3C and PreScission proteases"
                     /label=HRV 3C site
                     /translation="LEVLFQGP"
     CDS             1699..1722
                     /codon_start=1
                     /product="peptide that binds Strep-Tactin(R), an engineered
                     form of streptavidin"
                     /label=Strep-Tag II
                     /translation="WSHPQFEK"
     CDS             1759..1782
                     /codon_start=1
                     /product="peptide that binds Strep-Tactin(R), an engineered
                     form of streptavidin"
                     /label=Strep-Tag II
                     /translation="WSHPQFEK"
     polyA_signal    1942..2076
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(1979..1998)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     2033..2052
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     mobile_element  2197..2362
                     /mobile_element_type="transposon:Tn7"
                     /label=Tn7L
                     /note="mini-Tn7 element (left end of the Tn7 transposon)"
     rep_origin      2552..3140
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3041..3060
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     mobile_element  3275..3498
                     /mobile_element_type="transposon:Tn7"
                     /label=Tn7R
                     /note="mini-Tn7 element (right end of the Tn7 transposon)"
     CDS             complement(3565..4098)
                     /codon_start=1
                     /gene="aacC1"
                     /product="gentamycin acetyltransferase"
                     /label=GmR
                     /note="confers resistance to gentamycin"
                     /translation="MLRSSNDVTQQGSRPKTKLGGSSMGIIRTCRLGPDQVKSMRAALD
                     LFGREFGDVATYSQHQPDSDYLGNLLRSKTFIALAAFDQEAVVGALAAYVLPKFEQPRS
                     EIYIYDLAVSGEHRRQGIATALINLLKHEANALGAYVIYVQADYGDDPAVALYTKLGIR
                     EEVMHFDIDPSTAT"
     promoter        complement(join(4287..4292,4293..4309,4310..4315))
                     /gene="intI1 (promoter lies within the coding sequence)"
                     /label=Pc promoter
                     /note="class 1 integron promoter"
ORIGIN
        1 ataacttcgt atagcataca ttatacgaag ttatctgtaa ctataacggt cctaaggtag
       61 cgagtttaaa cactagtatc gattcgcgac ctactccgga atattaatag atcatggaga
      121 taattaaaat gataaccatc tcgcaaataa ataagtattt tactgttttc gtaacagttt
      181 tgtaataaaa aaacctataa atattccgga ttattcatac cgtcccacca tcgggcgcgg
      241 atccctcgag tcaacatggc gggcggagaa gctggagtga ctctagggca gccgcatctt
      301 tcgcgtcagg atctcaccac cttggatgtt accaagttga cgccactttc acacgaagtt
      361 atcagcagac aagccacaat taacataggt acaattggtc atgtagctca tgggaaatcc
      421 acagtcgtca aagctatttc tggagttcat actgtcaggt tcaaaaatga actagaaaga
      481 aatattacaa tcaagcttgg atatgctaat gctaagattt ataagcttga tgacccaagt
      541 tgccctcggc cagaatgtta tagatcttgt gggagcagta cacctgacga gtttcctacg
      601 gacattccag ggaccaaagg gaacttcaaa ttagtcagac atgtttcctt tgttgactgt
      661 cctggccacg atattttgat ggctactatg ctgaacggtg cagcagtgat ggatgcagct
      721 cttctgttga tagctggtaa tgaatcttgc cctcagcctc agacatcgga acacctggct
      781 gctatagaga tcatgaaact gaagcatatt ttgattctac aaaataaaat tgatttggta
      841 aaagaaagtc aggctaaaga acaatacgag cagatccttg catttgtcca aggtacagta
      901 gcagagggag ctcccattat tccaatttca gctcagctga aatacaatat tgaagttgtt
      961 tgtgagtaca tagtaaagaa aattccagta cccccaagag actttacttc agagccccgg
     1021 cttattgtta ttagatcttt tgatgtcaac aaacctggct gtgaagttga tgaccttaag
     1081 ggaggtgtag ctggtggtag tatcctaaaa ggagtattaa aggtgggcca ggagatagaa
     1141 gtaagacctg gtattgtttc caaagatagt gaaggaaaac tcatgtgtaa accaatcttt
     1201 tccaaaattg tatcactttt tgcggagcat aatgatctgc aatatgctgc tccaggcggt
     1261 cttattggag ttggaacaaa aattgacccc actttgtgcc gggctgacag aatggtgggg
     1321 caagtacttg gtgcagtcgg agctttacct gagatattca cagaattgga aatttcctat
     1381 ttcctgctta gacggcttct aggtgtacgc actgaaggag acaagaaagc agcaaaggtt
     1441 caaaagctgt ctaagaatga agtgctcatg gtgaacatag gatccctgtc aacaggaggg
     1501 agagttagtg ctgtcaaggc cgatttgggt aaaattgttt tgaccaatcc agtgtgcaca
     1561 gaggtaggag aaaaaattgc ccttagccga agagttgaaa aacactggcg tttaattggt
     1621 tggggtcaga taagaagagg agtgacaatc aagccaacag tagatgatga cggactcgaa
     1681 gtcctgtttc aaggtccctg gagccatcct cagttcgaga agggctccgc tggatctgct
     1741 gctggatctg gagctggttg gagccaccct cagtttgaga agtaaggtac ctctagagcc
     1801 tgcagtctcg acaagcttgt cgagaagtac tagaggatca taatcagcca taccacattt
     1861 gtagaggttt tacttgcttt aaaaaacctc ccacacctcc ccctgaacct gaaacataaa
     1921 atgaatgcaa ttgttgttgt taacttgttt attgcagctt ataatggtta caaataaagc
     1981 aatagcatca caaatttcac aaataaagca tttttttcac tgcattctag ttgtggtttg
     2041 tccaaactca tcaatgtatc ttatcatgtc tggatctgat cactgcttga gcctagaaga
     2101 tccggctgct aacaaagccc gaaaggaagc tgagttggct gctgccaccg ctgagcaata
     2161 actatcataa cccctagggt atacccatct aattggaacc agataagtga aatctagttc
     2221 caaactattt tgtcattttt aattttcgta ttagcttacg acgctacacc cagttcccat
     2281 ctattttgtc actcttccct aaataatcct taaaaactcc atttccaccc ctcccagttc
     2341 ccaactattt tgtccgccca caaccggttg acttgggtca actgtcagac caagtttact
     2401 catatatact ttagattgat ttaaaacttc atttttaatt taaaaggatc taggtgaaga
     2461 tcctttttga taatctcatg accaaaatcc cttaacgtga gttttcgttc cactgagcgt
     2521 cagaccccgt agaaaagatc aaaggatctt cttgagatcc tttttttctg cgcgtaatct
     2581 gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg gatcaagagc
     2641 taccaactct ttttccgaag gtaactggct tcagcagagc gcagatacca aatactgttc
     2701 ttctagtgta gccgtagtta ggccaccact tcaagaactc tgtagcaccg cctacatacc
     2761 tcgctctgct aatcctgtta ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg
     2821 ggttggactc aagacgatag ttaccggata aggcgcagcg gtcgggctga acggggggtt
     2881 cgtgcacaca gcccagcttg gagcgaacga cctacaccga actgagatac ctacagcgtg
     2941 agctatgaga aagcgccacg cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg
     3001 gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc tggtatcttt
     3061 atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag
     3121 gggggcggag cctatggaaa aacgccagca acgcggcctt tttacggttc ctggcctttt
     3181 gctggccttt tgctcacatg ttctttcctg cgttatcccc tgattgactt gggtcgctct
     3241 tcctgtggat gcgcagatgc cctgcgtaag cgggtgtggg cggacaataa agtcttaaac
     3301 tgaacaaaat agatctaaac tatgacaata aagtcttaaa ctagacagaa tagttgtaaa
     3361 ctgaaatcag tccagttatg ctgtgaaaaa gcatactgga cttttgttat ggctaaagca
     3421 aactcttcat tttctgaagt gcaaattgcc cgtcgtatta aagaggggcg tggccaaggg
     3481 catgtaaaga ctatattcgc ggcgttgtga caatttaccg aacaactccg cggccgggaa
     3541 gccgatctcg gcttgaacga attgttaggt ggcggtactt gggtcgatat caaagtgcat
     3601 cacttcttcc cgtatgccca actttgtata gagagccact gcgggatcgt caccgtaatc
     3661 tgcttgcacg tagatcacat aagcaccaag cgcgttggcc tcatgcttga ggagattgat
     3721 gagcgcggtg gcaatgccct gcctccggtg ctcgccggag actgcgagat catagatata
     3781 gatctcacta cgcggctgct caaacttggg cagaacgtaa gccgcgagag cgccaacaac
     3841 cgcttcttgg tcgaaggcag caagcgcgat gaatgtctta ctacggagca agttcccgag
     3901 gtaatcggag tccggctgat gttgggagta ggtggctacg tctccgaact cacgaccgaa
     3961 aagatcaaga gcagcccgca tggatttgac ttggtcaggg ccgagcctac atgtgcgaat
     4021 gatgcccata cttgagccac ctaactttgt tttagggcga ctgccctgct gcgtaacatc
     4081 gttgctgctg cgtaacatcg ttgctgctcc ataacatcaa acatcgaccc acggcgtaac
     4141 gcgcttgctg cttggatgcc cgaggcatag actgtacaaa aaaacagtca taacaagcca
     4201 tgaaaaccgc cactgcgccg ttaccaccgc tgcgttcggt caaggttctg gaccagttgc
     4261 gtgagcgcat acgctacttg cattacagtt tacgaaccga acaggcttat gtcaactggg
     4321 ttcgtgcctt catccgtttc cacggtgtgc gtcacccggc aaccttgggc agcagcgaag
     4381 tcgcc
//
