LOCUS       sequence-49618-e         767 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 767)
  AUTHORS   Shen A, Lupardus PJ, Morell M, Ponder EL, Sadaghiani AM, Garcia KC, 
            Bogyo M
  TITLE     Simplified, enhanced protein purification using an inducible, 
            autoprocessing enzyme tag.
  JOURNAL   PLoS One. 2009 Dec 2;4(12):e8119.
  PUBMED    19956581
REFERENCE   2  (bases 1 to 767)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2009-12-2"; volume: "4(12)"; pages: "e8119"
COMMENT     Sequence Label: pET22b-CPDSalI
FEATURES             Location/Qualifiers
     source          1..767
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(35..52)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
ORIGIN
        1 cttcctttcg ggctttgtta gcagccggat ctcagtggtg gtggtggtgg tgctcgagac
       61 cttgcgcgtc ccagcttagc gaaactttgt tgttttctgc cttttgaacc caatcgccat
      121 tcgcgtcctt ggtatgctta cgtcccgcct cgtctacggc cagttcagaa ctacgaacag
      181 agacatcgac acgaagacca ttcgcatcca tcgcgttaat aaactgatga ccaaagcctt
      241 tttgcttgtc gtcactcacc aaagaacaac caacaatact gatgtgatcc ggtttgttgt
      301 tgatgttttc ggcttgatta aacgactgtt ggaacttggc caatttcacg gccaactcat
      361 cggcactgta accacttaag cgagtattgt tagtttctga gtggtcgcga ccatgcccca
      421 ccaactgcca acgtagcttt ccatccagtt ttgacggatc gccatacacc acgcgatagt
      481 tgccgtctga atcgagctgc accaccacac tgctttcagc atgtttacct gctaaattgg
      541 ctgccgcttt tgctactacc gggtcgtttt ccatttgaac gatgatttga ccgtcgaagc
      601 gggtttcacc accatctgtc gttggtgtaa ccgtaatcgg gccccagcta ttaacatttt
      661 gattatggag tatttttcca tccgctaatg cgtcgacgga gctcgaattc ggatccgaat
      721 taattccgat atccatggcc anngccggct gggcagcgag gagcagc
//
