LOCUS       sequence-496186-       10093 bp    DNA     circular SYN 05-JAN-2026
DEFINITION  Expression of the human INSR tagged with Venus1 for BiFC .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10093)
  TITLE     Krejci lab plasmids 2025
REFERENCE   2  (bases 1 to 10093)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Jan 5, 2026 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pcDNA3.1-hINSR-Venus1
FEATURES             Location/Qualifiers
     source          1..10093
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(44..63)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        235..614
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        615..818
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     769..789
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     863..882
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        863..881
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             join(5179..5181,5182..5640)
                     /codon_start=1
                     /product="improved N-terminal fragment of mVenus for use in
                     bimolecular fluorescence complementation (BiFC) (Kodama and
                     Hu, 2010)"
                     /label=VN155(I152L)
                     /translation="VSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     LICTTGKLPVPWPTLVTTLGYGLQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYITA"
     primer_bind     complement(5221..5242)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(5482..5501)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     complement(5684..5701)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    5690..5914
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      5960..6388
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(6047..6066)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     6257..6278
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(6397..6417)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        6402..6731
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      6582..6717
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             6798..7592
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(6852..6871)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     7462..7481
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     polyA_signal    7766..7887
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7803..7822)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     7857..7876
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(7936..7952)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7936..7952)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(7949..7971)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    7960..7976
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(7984..7990,7991..8008,8009..8014))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8029..8050
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(8167..8184)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(8338..8926)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(8418..8437)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(9097..9888,9889..9957))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     9720..9739
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(9958..10062)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 gacggatcgg gagatctccc gatcccctat ggtgcactct cagtacaatc tgctctgatg
       61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgtg
      121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc
      181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt
      241 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata
      301 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc
      361 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc
      421 attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt
      481 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt
      541 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca
      601 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg
      661 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc
      721 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg
      781 gtaggcgtgt acggtgggag gtctatataa gcagagctct ctggctaact agagaaccca
      841 ctgcttactg gcttatcgaa attaatacga ctcactatag ggagacccaa gctggctagc
      901 aagctggtac cgagctcgga tccagtaccc ttcaccatgg gcaccggggg ccggcggggg
      961 gcggcggccg cgccgctgct ggtggcggtg gccgcgctgc tactgggcgc cgcgggccac
     1021 ctgtaccccg gagaggtgtg tcccggcatg gatatccgga acaacctcac taggttgcat
     1081 gagctggaga attgctctgt catcgaagga cacttgcaga tactcttgat gttcaaaacg
     1141 aggcccgaag atttccgaga cctcagtttc cccaaactca tcatgatcac tgattacttg
     1201 ctgctcttcc gggtctatgg gctcgagagc ctgaaggacc tgttccccaa cctcacggtc
     1261 atccggggat cacgactgtt ctttaactac gcgctggtca tcttcgagat ggttcacctc
     1321 aaggaactcg gcctctacaa cctgatgaac atcacccggg gttctgtccg catcgagaag
     1381 aacaatgagc tctgttactt ggccactatc gactggtccc gtatcctgga ttccgtggag
     1441 gataattaca tcgtgttgaa caaagatgac aacgaggagt gtggagacat ctgtccgggt
     1501 accgcgaagg gcaagaccaa ctgccccgcc accgtcatca acgggcagtt tgtcgaacga
     1561 tgttggactc atagtcactg ccagaaagtt tgcccgacca tctgtaagtc acacggctgc
     1621 accgccgaag gcctctgttg ccacagcgag tgcctgggca actgttctca gcccgacgac
     1681 cccaccaagt gcgtggcctg ccgcaacttc tacctggacg gcaggtgtgt ggagacctgc
     1741 ccgcccccgt actaccactt ccaggactgg cgctgtgtga acttcagctt ctgccaggac
     1801 ctgcaccaca aatgcaagaa ctcgcggagg cagggctgcc accagtacgt cattcacaac
     1861 aacaagtgca tccctgagtg tccctccggg tacacgatga attccagcaa cttgctgtgc
     1921 accccatgcc tgggtccctg tcccaaggtg tgccacctcc tagaaggcga gaagaccatc
     1981 gactcggtga cgtctgccca ggagctccga ggatgcaccg tcatcaacgg gagtctgatc
     2041 atcaacattc gaggaggcaa caatctggca gctgagctag aagccaacct cggcctcatt
     2101 gaagaaattt cagggtatct aaaaatccgc cgatcctacg ctctggtgtc actttccttc
     2161 ttccggaagt tacgtctgat tcgaggagag accttggaaa ttgggaacta ctccttctat
     2221 gccttggaca accagaacct aaggcagctc tgggactgga gcaaacacaa cctcaccatc
     2281 actcagggga aactcttctt ccactataac cccaaactct gcttgtcaga aatccacaag
     2341 atggaagaag tttcaggaac caaggggcgc caggagagaa acgacattgc cctgaagacc
     2401 aatggggacc aggcatcctg tgaaaatgag ttacttaaat tttcttacat tcggacatct
     2461 tttgacaaga tcttgctgag atgggagccg tactggcccc ccgacttccg agacctcttg
     2521 gggttcatgc tgttctacaa agaggcccct tatcagaatg tgacggagtt cgacgggcag
     2581 gatgcgtgtg gttccaacag ttggacggtg gtagacattg acccacccct gaggtccaac
     2641 gaccccaaat cacagaacca cccagggtgg ctgatgcggg gtctcaagcc ctggacccag
     2701 tatgccatct ttgtgaagac cctggtcacc ttttcggatg aacgccggac ctatggggcc
     2761 aagagtgaca tcatttatgt ccagacagat gccaccaacc cctctgtgcc cctggatcca
     2821 atctcagtgt ctaactcatc atcccagatt attctgaagt ggaaaccacc ctccgacccc
     2881 aatggcaaca tcacccacta cctggttttc tgggagaggc aggcggaaga cagtgagctg
     2941 ttcgagctgg attattgcct caaagggctg aagctgccct cgaggacctg gtctccacca
     3001 ttcgagtctg aagattctca gaagcacaac cagagtgagt atgaggattc ggccggcgaa
     3061 tgctgctcct gtccaaagac agactctcag atcctgaagg agctggagga gtcctcgttt
     3121 aggaagacgt ttgaggatta cctgcacaac gtggttttcg tccccagaaa aacctcttca
     3181 ggcactggtg ccgaggaccc taggccatct cggaaacgca ggtcccttgg cgatgttggg
     3241 aatgtgacgg tggccgtgcc cacggtggca gctttcccca acacttcctc gaccagcgtg
     3301 cccacgagtc cggaggagca caggcctttt gagaaggtgg tgaacaagga gtcgctggtc
     3361 atctccggct tgcgacactt cacgggctat cgcatcgagc tgcaggcttg caaccaggac
     3421 acccctgagg aacggtgcag tgtggcagcc tacgtcagtg cgaggaccat gcctgaagcc
     3481 aaggctgatg acattgttgg ccctgtgacg catgaaatct ttgagaacaa cgtcgtccac
     3541 ttgatgtggc aggagccgaa ggagcccaat ggtctgatcg tgctgtatga agtgagttat
     3601 cggcgatatg gtgatgagga gctgcatctc tgcgtctccc gcaagcactt cgctctggaa
     3661 cggggctgca ggctgcgtgg gctgtcaccg gggaactaca gcgtgcgaat ccgggccacc
     3721 tcccttgcgg gcaacggctc ttggacggaa cccacctatt tctacgtgac agactattta
     3781 gacgtcccgt caaatattgc aaaaattatc atcggccccc tcatctttgt ctttctcttc
     3841 agtgttgtga ttggaagtat ttatctattc ctgagaaaga ggcagccaga tgggccgctg
     3901 ggaccgcttt acgcttcttc aaaccctgag tatctcagtg ccagtgatgt gtttccatgc
     3961 tctgtgtacg tgccggacga gtgggaggtg tctcgagaga agatcaccct ccttcgagag
     4021 ctggggcagg gctccttcgg catggtgtat gagggcaatg ccagggacat catcaagggt
     4081 gaggcagaga cccgcgtggc ggtgaagacg gtcaacgagt cagccagtct ccgagagcgg
     4141 attgagttcc tcaatgaggc ctcggtcatg aagggcttca cctgccatca cgtggtgcgc
     4201 ctcctgggag tggtgtccaa gggccagccc acgctggtgg tgatggagct gatggctcac
     4261 ggagacctga agagctacct ccgttctctg cggccagagg ctgagaataa tcctggccgc
     4321 cctcccccta cccttcaaga gatgattcag atggcggcag agattgctga cgggatggcc
     4381 tacctgaacg ccaagaagtt tgtgcatcgg gacctggcag cgagaaactg catggtcgcc
     4441 catgatttta ctgtcaaaat tggagacttt ggaatgacca gagacatcta tgaaacggat
     4501 tactaccgga aagggggcaa gggtctgctc cctgtacggt ggatggcacc ggagtccctg
     4561 aaggatgggg tcttcaccac ttcttctgac atgtggtcct ttggcgtggt cctttgggaa
     4621 atcaccagct tggcagaaca gccttaccaa ggcctgtcta atgaacaggt gttgaaattt
     4681 gtcatggatg gagggtatct ggatcaaccc gacaactgtc cagagagagt cactgacctc
     4741 atgcgcatgt gctggcaatt caaccccaag atgaggccaa ccttcctgga gattgtcaac
     4801 ctgctcaagg acgacctgca ccccagcttt ccagaggtgt cgttcttcca cagcgaggag
     4861 aacaaggctc ccgagagtga ggagctggag atggagtttg aggacatgga gaatgtgccc
     4921 ctggaccgtt cctcgcactg tcagagggag gaggcggggg gccgggatgg agggtcctcg
     4981 ctgggtttca agcggagcta cgaggaacac atcccttaca cacacatgaa cggaggcaag
     5041 aaaaacgggc ggattctgac cttgcctcgg tccaatcctt ccgaattcgg aggaagtggc
     5101 ggaagtggcg gaagtggcgg aagtggagga agtgcaccgg ttggtggcgg tggctctgga
     5161 ggtggtgggt cctccggagt gagcaagggc gaggagctgt tcaccggggt ggtgcccatc
     5221 ctggtcgagc tggacggcga cgtaaacggc cacaagttca gcgtgtccgg cgagggcgag
     5281 ggcgatgcca cctacggcaa gctgaccctg aagctgatct gcaccaccgg caagctgccc
     5341 gtgccctggc ccaccctcgt gaccaccctg ggctacggcc tgcagtgctt cgcccgctac
     5401 cccgaccaca tgaagcagca cgacttcttc aagtccgcca tgcccgaagg ctacgtccag
     5461 gagcgcacca tcttcttcaa ggacgacggc aactacaaga cccgcgccga ggtgaagttc
     5521 gagggcgaca ccctggtgaa ccgcatcgag ctgaagggca tcgacttcaa ggaggacggc
     5581 aacatcctgg ggcacaagct ggagtacaac tacaacagcc acaacgtcta tatcaccgcc
     5641 gacaagcagt aatctagagg gcccgtttaa acccgctgat cagcctcgac tgtgccttct
     5701 agttgccagc catctgttgt ttgcccctcc cccgtgcctt ccttgaccct ggaaggtgcc
     5761 actcccactg tcctttccta ataaaatgag gaaattgcat cgcattgtct gagtaggtgt
     5821 cattctattc tggggggtgg ggtggggcag gacagcaagg gggaggattg ggaagacaat
     5881 agcaggcatg ctggggatgc ggtgggctct atggcttctg aggcggaaag aaccagctgg
     5941 ggctctaggg ggtatcccca cgcgccctgt agcggcgcat taagcgcggc gggtgtggtg
     6001 gttacgcgca gcgtgaccgc tacacttgcc agcgccctag cgcccgctcc tttcgctttc
     6061 ttcccttcct ttctcgccac gttcgccggc tttccccgtc aagctctaaa tcgggggctc
     6121 cctttagggt tccgatttag tgctttacgg cacctcgacc ccaaaaaact tgattagggt
     6181 gatggttcac gtagtgggcc atcgccctga tagacggttt ttcgcccttt gacgttggag
     6241 tccacgttct ttaatagtgg actcttgttc caaactggaa caacactcaa ccctatctcg
     6301 gtctattctt ttgatttata agggattttg ccgatttcgg cctattggtt aaaaaatgag
     6361 ctgatttaac aaaaatttaa cgcgaattaa ttctgtggaa tgtgtgtcag ttagggtgtg
     6421 gaaagtcccc aggctcccca gcaggcagaa gtatgcaaag catgcatctc aattagtcag
     6481 caaccaggtg tggaaagtcc ccaggctccc cagcaggcag aagtatgcaa agcatgcatc
     6541 tcaattagtc agcaaccata gtcccgcccc taactccgcc catcccgccc ctaactccgc
     6601 ccagttccgc ccattctccg ccccatggct gactaatttt ttttatttat gcagaggccg
     6661 aggccgcctc tgcctctgag ctattccaga agtagtgagg aggctttttt ggaggcctag
     6721 gcttttgcaa aaagctcccg ggagcttgta tatccatttt cggatctgat caagagacag
     6781 gatgaggatc gtttcgcatg attgaacaag atggattgca cgcaggttct ccggccgctt
     6841 gggtggagag gctattcggc tatgactggg cacaacagac aatcggctgc tctgatgccg
     6901 ccgtgttccg gctgtcagcg caggggcgcc cggttctttt tgtcaagacc gacctgtccg
     6961 gtgccctgaa tgaactgcag gacgaggcag cgcggctatc gtggctggcc acgacgggcg
     7021 ttccttgcgc agctgtgctc gacgttgtca ctgaagcggg aagggactgg ctgctattgg
     7081 gcgaagtgcc ggggcaggat ctcctgtcat ctcaccttgc tcctgccgag aaagtatcca
     7141 tcatggctga tgcaatgcgg cggctgcata cgcttgatcc ggctacctgc ccattcgacc
     7201 accaagcgaa acatcgcatc gagcgagcac gtactcggat ggaagccggt cttgtcgatc
     7261 aggatgatct ggacgaagag catcaggggc tcgcgccagc cgaactgttc gccaggctca
     7321 aggcgcgcat gcccgacggc gaggatctcg tcgtgaccca tggcgatgcc tgcttgccga
     7381 atatcatggt ggaaaatggc cgcttttctg gattcatcga ctgtggccgg ctgggtgtgg
     7441 cggaccgcta tcaggacata gcgttggcta cccgtgatat tgctgaagag cttggcggcg
     7501 aatgggctga ccgcttcctc gtgctttacg gtatcgccgc tcccgattcg cagcgcatcg
     7561 ccttctatcg ccttcttgac gagttcttct gagcgggact ctggggttcg aaatgaccga
     7621 ccaagcgacg cccaacctgc catcacgaga tttcgattcc accgccgcct tctatgaaag
     7681 gttgggcttc ggaatcgttt tccgggacgc cggctggatg atcctccagc gcggggatct
     7741 catgctggag ttcttcgccc accccaactt gtttattgca gcttataatg gttacaaata
     7801 aagcaatagc atcacaaatt tcacaaataa agcatttttt tcactgcatt ctagttgtgg
     7861 tttgtccaaa ctcatcaatg tatcttatca tgtctgtata ccgtcgacct ctagctagag
     7921 cttggcgtaa tcatggtcat agctgtttcc tgtgtgaaat tgttatccgc tcacaattcc
     7981 acacaacata cgagccggaa gcataaagtg taaagcctgg ggtgcctaat gagtgagcta
     8041 actcacatta attgcgttgc gctcactgcc cgctttccag tcgggaaacc tgtcgtgcca
     8101 gctgcattaa tgaatcggcc aacgcgcggg gagaggcggt ttgcgtattg ggcgctcttc
     8161 cgcttcctcg ctcactgact cgctgcgctc ggtcgttcgg ctgcggcgag cggtatcagc
     8221 tcactcaaag gcggtaatac ggttatccac agaatcaggg gataacgcag gaaagaacat
     8281 gtgagcaaaa ggccagcaaa aggccaggaa ccgtaaaaag gccgcgttgc tggcgttttt
     8341 ccataggctc cgcccccctg acgagcatca caaaaatcga cgctcaagtc agaggtggcg
     8401 aaacccgaca ggactataaa gataccaggc gtttccccct ggaagctccc tcgtgcgctc
     8461 tcctgttccg accctgccgc ttaccggata cctgtccgcc tttctccctt cgggaagcgt
     8521 ggcgctttct catagctcac gctgtaggta tctcagttcg gtgtaggtcg ttcgctccaa
     8581 gctgggctgt gtgcacgaac cccccgttca gcccgaccgc tgcgccttat ccggtaacta
     8641 tcgtcttgag tccaacccgg taagacacga cttatcgcca ctggcagcag ccactggtaa
     8701 caggattagc agagcgaggt atgtaggcgg tgctacagag ttcttgaagt ggtggcctaa
     8761 ctacggctac actagaagaa cagtatttgg tatctgcgct ctgctgaagc cagttacctt
     8821 cggaaaaaga gttggtagct cttgatccgg caaacaaacc accgctggta gcggtggttt
     8881 ttttgtttgc aagcagcaga ttacgcgcag aaaaaaagga tctcaagaag atcctttgat
     8941 cttttctacg gggtctgacg ctcagtggaa cgaaaactca cgttaaggga ttttggtcat
     9001 gagattatca aaaaggatct tcacctagat ccttttaaat taaaaatgaa gttttaaatc
     9061 aatctaaagt atatatgagt aaacttggtc tgacagttac caatgcttaa tcagtgaggc
     9121 acctatctca gcgatctgtc tatttcgttc atccatagtt gcctgactcc ccgtcgtgta
     9181 gataactacg atacgggagg gcttaccatc tggccccagt gctgcaatga taccgcgaga
     9241 cccacgctca ccggctccag atttatcagc aataaaccag ccagccggaa gggccgagcg
     9301 cagaagtggt cctgcaactt tatccgcctc catccagtct attaattgtt gccgggaagc
     9361 tagagtaagt agttcgccag ttaatagttt gcgcaacgtt gttgccattg ctacaggcat
     9421 cgtggtgtca cgctcgtcgt ttggtatggc ttcattcagc tccggttccc aacgatcaag
     9481 gcgagttaca tgatccccca tgttgtgcaa aaaagcggtt agctccttcg gtcctccgat
     9541 cgttgtcaga agtaagttgg ccgcagtgtt atcactcatg gttatggcag cactgcataa
     9601 ttctcttact gtcatgccat ccgtaagatg cttttctgtg actggtgagt actcaaccaa
     9661 gtcattctga gaatagtgta tgcggcgacc gagttgctct tgcccggcgt caatacggga
     9721 taataccgcg ccacatagca gaactttaaa agtgctcatc attggaaaac gttcttcggg
     9781 gcgaaaactc tcaaggatct taccgctgtt gagatccagt tcgatgtaac ccactcgtgc
     9841 acccaactga tcttcagcat cttttacttt caccagcgtt tctgggtgag caaaaacagg
     9901 aaggcaaaat gccgcaaaaa agggaataag ggcgacacgg aaatgttgaa tactcatact
     9961 cttccttttt caatattatt gaagcattta tcagggttat tgtctcatga gcggatacat
    10021 atttgaatgt atttagaaaa ataaacaaat aggggttccg cgcacatttc cccgaaaagt
    10081 gccacctgac gtc
//
