LOCUS       sequence-496189-        9865 bp    DNA     circular SYN 05-JAN-2026
DEFINITION  Expression of the human INSR tagged with Venus2 for BiFC .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9865)
  TITLE     Krejci lab plasmids 2025
REFERENCE   2  (bases 1 to 9865)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Jan 5, 2026 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pcDNA3.1-hINSR-Venus2
FEATURES             Location/Qualifiers
     source          1..9865
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(44..63)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        235..614
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        615..818
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     769..789
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     863..882
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        863..881
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     5358..5379
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     complement(5456..5473)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    5462..5686
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      5732..6160
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(5819..5838)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     6029..6050
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(6169..6189)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        6174..6503
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      6354..6489
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             6570..7364
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(6624..6643)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     7234..7253
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     polyA_signal    7538..7659
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7575..7594)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     7629..7648
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(7708..7724)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7708..7724)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(7721..7743)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    7732..7748
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(7756..7762,7763..7780,7781..7786))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7801..7822
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(7939..7956)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(8110..8698)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(8190..8209)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(8869..9660,9661..9729))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     9492..9511
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(9730..9834)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 gacggatcgg gagatctccc gatcccctat ggtgcactct cagtacaatc tgctctgatg
       61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgtg
      121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc
      181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt
      241 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata
      301 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc
      361 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc
      421 attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt
      481 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt
      541 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca
      601 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg
      661 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc
      721 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg
      781 gtaggcgtgt acggtgggag gtctatataa gcagagctct ctggctaact agagaaccca
      841 ctgcttactg gcttatcgaa attaatacga ctcactatag ggagacccaa gctggctagc
      901 aagctggtac cgagctcgga tccagtaccc ttcaccatgg gcaccggggg ccggcggggg
      961 gcggcggccg cgccgctgct ggtggcggtg gccgcgctgc tactgggcgc cgcgggccac
     1021 ctgtaccccg gagaggtgtg tcccggcatg gatatccgga acaacctcac taggttgcat
     1081 gagctggaga attgctctgt catcgaagga cacttgcaga tactcttgat gttcaaaacg
     1141 aggcccgaag atttccgaga cctcagtttc cccaaactca tcatgatcac tgattacttg
     1201 ctgctcttcc gggtctatgg gctcgagagc ctgaaggacc tgttccccaa cctcacggtc
     1261 atccggggat cacgactgtt ctttaactac gcgctggtca tcttcgagat ggttcacctc
     1321 aaggaactcg gcctctacaa cctgatgaac atcacccggg gttctgtccg catcgagaag
     1381 aacaatgagc tctgttactt ggccactatc gactggtccc gtatcctgga ttccgtggag
     1441 gataattaca tcgtgttgaa caaagatgac aacgaggagt gtggagacat ctgtccgggt
     1501 accgcgaagg gcaagaccaa ctgccccgcc accgtcatca acgggcagtt tgtcgaacga
     1561 tgttggactc atagtcactg ccagaaagtt tgcccgacca tctgtaagtc acacggctgc
     1621 accgccgaag gcctctgttg ccacagcgag tgcctgggca actgttctca gcccgacgac
     1681 cccaccaagt gcgtggcctg ccgcaacttc tacctggacg gcaggtgtgt ggagacctgc
     1741 ccgcccccgt actaccactt ccaggactgg cgctgtgtga acttcagctt ctgccaggac
     1801 ctgcaccaca aatgcaagaa ctcgcggagg cagggctgcc accagtacgt cattcacaac
     1861 aacaagtgca tccctgagtg tccctccggg tacacgatga attccagcaa cttgctgtgc
     1921 accccatgcc tgggtccctg tcccaaggtg tgccacctcc tagaaggcga gaagaccatc
     1981 gactcggtga cgtctgccca ggagctccga ggatgcaccg tcatcaacgg gagtctgatc
     2041 atcaacattc gaggaggcaa caatctggca gctgagctag aagccaacct cggcctcatt
     2101 gaagaaattt cagggtatct aaaaatccgc cgatcctacg ctctggtgtc actttccttc
     2161 ttccggaagt tacgtctgat tcgaggagag accttggaaa ttgggaacta ctccttctat
     2221 gccttggaca accagaacct aaggcagctc tgggactgga gcaaacacaa cctcaccatc
     2281 actcagggga aactcttctt ccactataac cccaaactct gcttgtcaga aatccacaag
     2341 atggaagaag tttcaggaac caaggggcgc caggagagaa acgacattgc cctgaagacc
     2401 aatggggacc aggcatcctg tgaaaatgag ttacttaaat tttcttacat tcggacatct
     2461 tttgacaaga tcttgctgag atgggagccg tactggcccc ccgacttccg agacctcttg
     2521 gggttcatgc tgttctacaa agaggcccct tatcagaatg tgacggagtt cgacgggcag
     2581 gatgcgtgtg gttccaacag ttggacggtg gtagacattg acccacccct gaggtccaac
     2641 gaccccaaat cacagaacca cccagggtgg ctgatgcggg gtctcaagcc ctggacccag
     2701 tatgccatct ttgtgaagac cctggtcacc ttttcggatg aacgccggac ctatggggcc
     2761 aagagtgaca tcatttatgt ccagacagat gccaccaacc cctctgtgcc cctggatcca
     2821 atctcagtgt ctaactcatc atcccagatt attctgaagt ggaaaccacc ctccgacccc
     2881 aatggcaaca tcacccacta cctggttttc tgggagaggc aggcggaaga cagtgagctg
     2941 ttcgagctgg attattgcct caaagggctg aagctgccct cgaggacctg gtctccacca
     3001 ttcgagtctg aagattctca gaagcacaac cagagtgagt atgaggattc ggccggcgaa
     3061 tgctgctcct gtccaaagac agactctcag atcctgaagg agctggagga gtcctcgttt
     3121 aggaagacgt ttgaggatta cctgcacaac gtggttttcg tccccagaaa aacctcttca
     3181 ggcactggtg ccgaggaccc taggccatct cggaaacgca ggtcccttgg cgatgttggg
     3241 aatgtgacgg tggccgtgcc cacggtggca gctttcccca acacttcctc gaccagcgtg
     3301 cccacgagtc cggaggagca caggcctttt gagaaggtgg tgaacaagga gtcgctggtc
     3361 atctccggct tgcgacactt cacgggctat cgcatcgagc tgcaggcttg caaccaggac
     3421 acccctgagg aacggtgcag tgtggcagcc tacgtcagtg cgaggaccat gcctgaagcc
     3481 aaggctgatg acattgttgg ccctgtgacg catgaaatct ttgagaacaa cgtcgtccac
     3541 ttgatgtggc aggagccgaa ggagcccaat ggtctgatcg tgctgtatga agtgagttat
     3601 cggcgatatg gtgatgagga gctgcatctc tgcgtctccc gcaagcactt cgctctggaa
     3661 cggggctgca ggctgcgtgg gctgtcaccg gggaactaca gcgtgcgaat ccgggccacc
     3721 tcccttgcgg gcaacggctc ttggacggaa cccacctatt tctacgtgac agactattta
     3781 gacgtcccgt caaatattgc aaaaattatc atcggccccc tcatctttgt ctttctcttc
     3841 agtgttgtga ttggaagtat ttatctattc ctgagaaaga ggcagccaga tgggccgctg
     3901 ggaccgcttt acgcttcttc aaaccctgag tatctcagtg ccagtgatgt gtttccatgc
     3961 tctgtgtacg tgccggacga gtgggaggtg tctcgagaga agatcaccct ccttcgagag
     4021 ctggggcagg gctccttcgg catggtgtat gagggcaatg ccagggacat catcaagggt
     4081 gaggcagaga cccgcgtggc ggtgaagacg gtcaacgagt cagccagtct ccgagagcgg
     4141 attgagttcc tcaatgaggc ctcggtcatg aagggcttca cctgccatca cgtggtgcgc
     4201 ctcctgggag tggtgtccaa gggccagccc acgctggtgg tgatggagct gatggctcac
     4261 ggagacctga agagctacct ccgttctctg cggccagagg ctgagaataa tcctggccgc
     4321 cctcccccta cccttcaaga gatgattcag atggcggcag agattgctga cgggatggcc
     4381 tacctgaacg ccaagaagtt tgtgcatcgg gacctggcag cgagaaactg catggtcgcc
     4441 catgatttta ctgtcaaaat tggagacttt ggaatgacca gagacatcta tgaaacggat
     4501 tactaccgga aagggggcaa gggtctgctc cctgtacggt ggatggcacc ggagtccctg
     4561 aaggatgggg tcttcaccac ttcttctgac atgtggtcct ttggcgtggt cctttgggaa
     4621 atcaccagct tggcagaaca gccttaccaa ggcctgtcta atgaacaggt gttgaaattt
     4681 gtcatggatg gagggtatct ggatcaaccc gacaactgtc cagagagagt cactgacctc
     4741 atgcgcatgt gctggcaatt caaccccaag atgaggccaa ccttcctgga gattgtcaac
     4801 ctgctcaagg acgacctgca ccccagcttt ccagaggtgt cgttcttcca cagcgaggag
     4861 aacaaggctc ccgagagtga ggagctggag atggagtttg aggacatgga gaatgtgccc
     4921 ctggaccgtt cctcgcactg tcagagggag gaggcggggg gccgggatgg agggtcctcg
     4981 ctgggtttca agcggagcta cgaggaacac atcccttaca cacacatgaa cggaggcaag
     5041 aaaaacgggc ggattctgac cttgcctcgg tccaatcctt ccgaattcgg aggaagtggc
     5101 ggaagtggcg gaagtggcgg aagtggagga agtgcaccgg ttggtggcgg tggctctgga
     5161 ggtggtgggt cctccggaaa gaacggcatc aaggccaact tcaagatccg ccacaacatc
     5221 gaggacggcg gcgtgcagct cgccgaccac taccagcaga acacccccat cggcgacggc
     5281 cccgtgctgc tgcccgacaa ccactacctg agctaccagt ccgccctgag caaagacccc
     5341 aacgagaagc gcgatcacat ggtcctgctg gagttcgtga ccgccgccgg gatcactctc
     5401 ggcatggacg agctgtacaa gtaatctaga gggcccgttt aaacccgctg atcagcctcg
     5461 actgtgcctt ctagttgcca gccatctgtt gtttgcccct cccccgtgcc ttccttgacc
     5521 ctggaaggtg ccactcccac tgtcctttcc taataaaatg aggaaattgc atcgcattgt
     5581 ctgagtaggt gtcattctat tctggggggt ggggtggggc aggacagcaa gggggaggat
     5641 tgggaagaca atagcaggca tgctggggat gcggtgggct ctatggcttc tgaggcggaa
     5701 agaaccagct ggggctctag ggggtatccc cacgcgccct gtagcggcgc attaagcgcg
     5761 gcgggtgtgg tggttacgcg cagcgtgacc gctacacttg ccagcgccct agcgcccgct
     5821 cctttcgctt tcttcccttc ctttctcgcc acgttcgccg gctttccccg tcaagctcta
     5881 aatcgggggc tccctttagg gttccgattt agtgctttac ggcacctcga ccccaaaaaa
     5941 cttgattagg gtgatggttc acgtagtggg ccatcgccct gatagacggt ttttcgccct
     6001 ttgacgttgg agtccacgtt ctttaatagt ggactcttgt tccaaactgg aacaacactc
     6061 aaccctatct cggtctattc ttttgattta taagggattt tgccgatttc ggcctattgg
     6121 ttaaaaaatg agctgattta acaaaaattt aacgcgaatt aattctgtgg aatgtgtgtc
     6181 agttagggtg tggaaagtcc ccaggctccc cagcaggcag aagtatgcaa agcatgcatc
     6241 tcaattagtc agcaaccagg tgtggaaagt ccccaggctc cccagcaggc agaagtatgc
     6301 aaagcatgca tctcaattag tcagcaacca tagtcccgcc cctaactccg cccatcccgc
     6361 ccctaactcc gcccagttcc gcccattctc cgccccatgg ctgactaatt ttttttattt
     6421 atgcagaggc cgaggccgcc tctgcctctg agctattcca gaagtagtga ggaggctttt
     6481 ttggaggcct aggcttttgc aaaaagctcc cgggagcttg tatatccatt ttcggatctg
     6541 atcaagagac aggatgagga tcgtttcgca tgattgaaca agatggattg cacgcaggtt
     6601 ctccggccgc ttgggtggag aggctattcg gctatgactg ggcacaacag acaatcggct
     6661 gctctgatgc cgccgtgttc cggctgtcag cgcaggggcg cccggttctt tttgtcaaga
     6721 ccgacctgtc cggtgccctg aatgaactgc aggacgaggc agcgcggcta tcgtggctgg
     6781 ccacgacggg cgttccttgc gcagctgtgc tcgacgttgt cactgaagcg ggaagggact
     6841 ggctgctatt gggcgaagtg ccggggcagg atctcctgtc atctcacctt gctcctgccg
     6901 agaaagtatc catcatggct gatgcaatgc ggcggctgca tacgcttgat ccggctacct
     6961 gcccattcga ccaccaagcg aaacatcgca tcgagcgagc acgtactcgg atggaagccg
     7021 gtcttgtcga tcaggatgat ctggacgaag agcatcaggg gctcgcgcca gccgaactgt
     7081 tcgccaggct caaggcgcgc atgcccgacg gcgaggatct cgtcgtgacc catggcgatg
     7141 cctgcttgcc gaatatcatg gtggaaaatg gccgcttttc tggattcatc gactgtggcc
     7201 ggctgggtgt ggcggaccgc tatcaggaca tagcgttggc tacccgtgat attgctgaag
     7261 agcttggcgg cgaatgggct gaccgcttcc tcgtgcttta cggtatcgcc gctcccgatt
     7321 cgcagcgcat cgccttctat cgccttcttg acgagttctt ctgagcggga ctctggggtt
     7381 cgaaatgacc gaccaagcga cgcccaacct gccatcacga gatttcgatt ccaccgccgc
     7441 cttctatgaa aggttgggct tcggaatcgt tttccgggac gccggctgga tgatcctcca
     7501 gcgcggggat ctcatgctgg agttcttcgc ccaccccaac ttgtttattg cagcttataa
     7561 tggttacaaa taaagcaata gcatcacaaa tttcacaaat aaagcatttt tttcactgca
     7621 ttctagttgt ggtttgtcca aactcatcaa tgtatcttat catgtctgta taccgtcgac
     7681 ctctagctag agcttggcgt aatcatggtc atagctgttt cctgtgtgaa attgttatcc
     7741 gctcacaatt ccacacaaca tacgagccgg aagcataaag tgtaaagcct ggggtgccta
     7801 atgagtgagc taactcacat taattgcgtt gcgctcactg cccgctttcc agtcgggaaa
     7861 cctgtcgtgc cagctgcatt aatgaatcgg ccaacgcgcg gggagaggcg gtttgcgtat
     7921 tgggcgctct tccgcttcct cgctcactga ctcgctgcgc tcggtcgttc ggctgcggcg
     7981 agcggtatca gctcactcaa aggcggtaat acggttatcc acagaatcag gggataacgc
     8041 aggaaagaac atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa aggccgcgtt
     8101 gctggcgttt ttccataggc tccgcccccc tgacgagcat cacaaaaatc gacgctcaag
     8161 tcagaggtgg cgaaacccga caggactata aagataccag gcgtttcccc ctggaagctc
     8221 cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga tacctgtccg cctttctccc
     8281 ttcgggaagc gtggcgcttt ctcatagctc acgctgtagg tatctcagtt cggtgtaggt
     8341 cgttcgctcc aagctgggct gtgtgcacga accccccgtt cagcccgacc gctgcgcctt
     8401 atccggtaac tatcgtcttg agtccaaccc ggtaagacac gacttatcgc cactggcagc
     8461 agccactggt aacaggatta gcagagcgag gtatgtaggc ggtgctacag agttcttgaa
     8521 gtggtggcct aactacggct acactagaag aacagtattt ggtatctgcg ctctgctgaa
     8581 gccagttacc ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg
     8641 tagcggtggt ttttttgttt gcaagcagca gattacgcgc agaaaaaaag gatctcaaga
     8701 agatcctttg atcttttcta cggggtctga cgctcagtgg aacgaaaact cacgttaagg
     8761 gattttggtc atgagattat caaaaaggat cttcacctag atccttttaa attaaaaatg
     8821 aagttttaaa tcaatctaaa gtatatatga gtaaacttgg tctgacagtt accaatgctt
     8881 aatcagtgag gcacctatct cagcgatctg tctatttcgt tcatccatag ttgcctgact
     8941 ccccgtcgtg tagataacta cgatacggga gggcttacca tctggcccca gtgctgcaat
     9001 gataccgcga gacccacgct caccggctcc agatttatca gcaataaacc agccagccgg
     9061 aagggccgag cgcagaagtg gtcctgcaac tttatccgcc tccatccagt ctattaattg
     9121 ttgccgggaa gctagagtaa gtagttcgcc agttaatagt ttgcgcaacg ttgttgccat
     9181 tgctacaggc atcgtggtgt cacgctcgtc gtttggtatg gcttcattca gctccggttc
     9241 ccaacgatca aggcgagtta catgatcccc catgttgtgc aaaaaagcgg ttagctcctt
     9301 cggtcctccg atcgttgtca gaagtaagtt ggccgcagtg ttatcactca tggttatggc
     9361 agcactgcat aattctctta ctgtcatgcc atccgtaaga tgcttttctg tgactggtga
     9421 gtactcaacc aagtcattct gagaatagtg tatgcggcga ccgagttgct cttgcccggc
     9481 gtcaatacgg gataataccg cgccacatag cagaacttta aaagtgctca tcattggaaa
     9541 acgttcttcg gggcgaaaac tctcaaggat cttaccgctg ttgagatcca gttcgatgta
     9601 acccactcgt gcacccaact gatcttcagc atcttttact ttcaccagcg tttctgggtg
     9661 agcaaaaaca ggaaggcaaa atgccgcaaa aaagggaata agggcgacac ggaaatgttg
     9721 aatactcata ctcttccttt ttcaatatta ttgaagcatt tatcagggtt attgtctcat
     9781 gagcggatac atatttgaat gtatttagaa aaataaacaa ataggggttc cgcgcacatt
     9841 tccccgaaaa gtgccacctg acgtc
//
