LOCUS       sequence-49619-e         664 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 664)
  AUTHORS   Miller WG, Leveau JH, Lindow SE
  TITLE     Improved gfp and inaZ broad-host-range promoter-probe vectors.
  JOURNAL   Mol Plant Microbe Interact. 2000 Nov;13(11):1243-50.
  PUBMED    11059491
REFERENCE   2  (bases 1 to 664)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Plant 
            Microbe Interact."; date: "2000-11"; volume: "13(11)"; pages: 
            "1243-50"
COMMENT     Sequence Label: pPROBE-TT
FEATURES             Location/Qualifiers
     source          1..664
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     misc_feature    67..123
                     /label=MCS
                     /note="pUC18/19 multiple cloning site"
     terminator      complement(215..301)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     terminator      complement(398..484)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     terminator      complement(581..663)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
ORIGIN
        1 cttctccttt actcatatgt ttttcctcct tataaagtta atctttagtt agttagggaa
       61 taagccgaat tcgagctcgg tacccgggga tcctctagag tcgacctgca ggcatgcaag
      121 cttccgatcc ccaattcctg gcagtttatg gcgggcgtcc tgcccgccac cctccgggcc
      181 gttgcttcgc aacgttcaaa tccgctcccg gcggatttgt cctactcagg agagcgttca
      241 ccgacaaaca acagataaaa cgaaaggccc agtctttcga ctgagccttt cgttttattt
      301 gatgcctggg gaattaattc ctggcagttt atggcgggcg tcctgcccgc caccctccgg
      361 gccgttgctt cgcaacgttc aaatccgctc ccggcggatt tgtcctactc aggagagcgt
      421 tcaccgacaa acaacagata aaacgaaagg cccagtcttt cgactgagcc tttcgtttta
      481 tttgatgcct ggggaattaa ttcctggcag tttatggcgg gcgtcctgcc cgccaccctc
      541 cgggccgttg cttcgcaacg ttcaaatccg ctcccggcgg atttgtccta ctcaggagag
      601 cgttcaccga caaacaacag ataaaaacga aaggcccagt ctttcgactg agcctttcgt
      661 ttta
//
