LOCUS       sequence-496190-        8117 bp    DNA     circular SYN 05-JAN-2026
DEFINITION  Expression of the human FGFR1c tagged with Venus2 for BiFC .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8117)
  TITLE     Krejci lab plasmids 2025
REFERENCE   2  (bases 1 to 8117)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Jan 5, 2026 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pcDNA3.1-hFGFR1c-Venus2
FEATURES             Location/Qualifiers
     source          1..8117
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(44..63)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        235..614
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        615..818
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     769..789
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     863..882
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        863..881
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             complement(1302..1319)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     3610..3631
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     complement(3708..3725)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    3714..3938
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      3984..4412
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(4071..4090)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     4281..4302
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(4421..4441)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        4426..4755
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      4606..4741
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     4668..4687
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     CDS             4822..5616
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(4876..4895)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     5486..5505
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     polyA_signal    5790..5911
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5827..5846)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     5881..5900
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(5960..5976)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5960..5976)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(5973..5995)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    5984..6000
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(6008..6014,6015..6032,6033..6038))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6053..6074
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(6191..6208)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(6362..6950)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(6442..6461)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(7121..7912,7913..7981))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     7744..7763
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(7982..8086)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 gacggatcgg gagatctccc gatcccctat ggtgcactct cagtacaatc tgctctgatg
       61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg
      121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc
      181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt
      241 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata
      301 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc
      361 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc
      421 attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt
      481 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt
      541 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca
      601 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg
      661 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc
      721 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg
      781 gtaggcgtgt acggtgggag gtctatataa gcagagctct ctggctaact agagaaccca
      841 ctgcttactg gcttatcgaa attaatacga ctcactatag ggagacccaa gctggctagc
      901 gtttaaactt aagcttgccg ccatgtggag ctggaagtgc ctcctcttct gggctgtgct
      961 ggtcacagcc acactctgca ccgctaggcc gtccccgacc ttgcctgaac aagcccagcc
     1021 ctggggagcc cctgtggaag tggagtcctt cctggtccac cccggtgacc tgctgcagct
     1081 tcgctgtcgg ctgcgggacg atgtgcagag catcaactgg ctgcgggacg gggtgcagct
     1141 ggcggaaagc aaccgcaccc gcatcacagg ggaggaggtg gaggtgcagg actccgtgcc
     1201 cgcagactcc ggcctctatg cttgcgtaac cagcagcccc tcgggcagtg acaccaccta
     1261 cttctccgtc aatgtttcag atgctctccc ctcctcggag gatgatgatg atgatgatga
     1321 ctcctcttca gaggagaaag aaacagataa caccaaacca aaccgtatgc ccgtagctcc
     1381 atattggaca tccccagaaa agatggaaaa gaaattgcat gcagtgccgg ctgccaagac
     1441 agtgaagttc aaatgccctt ccagtgggac cccaaacccc acactgcgct ggttgaaaaa
     1501 tggcaaagaa ttcaaacctg accacagaat tggaggctac aaggtccgtt atgccacctg
     1561 gagcatcata atggactctg tggtgccctc tgacaagggc aactacacct gcattgtgga
     1621 gaatgagtac ggcagcatca accacacata ccagctggat gtcgtggagc ggtcccctca
     1681 ccggcccatc ctgcaagcag ggttgcccgc caacaaaaca gtggccctgg gtagcaacgt
     1741 ggagttcatg tgtaaggtgt acagtgaccc gcagccgcac atccagtggc taaagcacat
     1801 cgaggtgaat gggagcaaga ttggcccaga caacctgcct tatgtccaga tcttgaagac
     1861 tgctggagtt aataccaccg acaaagagat ggaggtgctt cacttaagaa atgtctcctt
     1921 tgaggacgca ggggagtata cgtgcttggc gggtaactct atcggactct cccatcactc
     1981 tgcatggttg accgttctgg aagccctgga agagaggccg gcagtgatga cctcgcccct
     2041 gtacctggag atcatcatct attgcacagg ggccttcctc atctcctgca tggtggggtc
     2101 ggtcatcgtc tacaagatga agagtggtac caagaagagt gacttccaca gccagatggc
     2161 tgtgcacaag ctggccaaga gcatccctct gcgcagacag gtaacagtgt ctgctgactc
     2221 cagtgcatcc atgaactctg gggttcttct ggttcggcca tcacggctct cctccagtgg
     2281 gactcccatg ctagcagggg tctctgagta tgagcttccc gaagaccctc gctgggagct
     2341 gcctcgggac agactggtct taggcaaacc cctgggagag ggctgctttg ggcaggtggt
     2401 gttggcagag gctatcgggc tggacaagga caaacccaac cgtgtgacca aagtggctgt
     2461 gaagatgttg aagtcggacg caacagagaa agacttgtca gacctgatct cagaaatgga
     2521 gatgatgaag atgatcggga agcataagaa tatcatcaac ctgctggggg cctgcacgca
     2581 ggatggtccc ttgtatgtca tcgtggagta tgcctccaag ggcaacctgc gggagtacct
     2641 gcaggcccgg aggcccccag ggctggaata ctgctacaac cccagccaca acccagagga
     2701 gcagctctcc tccaaggacc tggtgtcctg cgcctaccag gtggcccgag gcatggagta
     2761 tctggcctcc aagaagtgca tacaccgaga cctggcagcc aggaatgtcc tggtgacaga
     2821 ggacaatgtg atgaagatag cagactttgg cctcgcacgg gacattcacc acatcgacta
     2881 ctataaaaag acaaccaacg gccgactgcc tgtgaagtgg atggcacccg aggcattatt
     2941 tgaccggatc tacacccacc agagtgatgt gtggtctttc ggggtgctcc tgtgggagat
     3001 cttcactctg ggcggctccc cataccccgg tgtgcctgtg gaggaacttt tcaagctgct
     3061 gaaggagggt caccgcatgg acaagcccag taactgcacc aacgagctgt acatgatgat
     3121 gcgggactgc tggcatgcag tgccctcaca gagacccacc ttcaagcagc tggtggaaga
     3181 cctggaccgc atcgtggcct tgacctccaa ccaggagtac ctggacctgt ccatgcccct
     3241 ggaccagtac tcccccagct ttcccgacac ccggagctct acgtgctcct caggggagga
     3301 ttccgtcttc tctcatgagc cgctgcccga ggagccctgc ctgccccgac acccagccca
     3361 gcttgccaat ggcggactca aacgccgcct cgagggtggc ggtggctctg gaggtggtgg
     3421 gtcctccgga aagaacggca tcaaggccaa cttcaagatc cgccacaaca tcgaggacgg
     3481 cggcgtgcag ctcgccgacc actaccagca gaacaccccc atcggcgacg gccccgtgct
     3541 gctgcccgac aaccactacc tgagctacca gtccgccctg agcaaagacc ccaacgagaa
     3601 gcgcgatcac atggtcctgc tggagttcgt gaccgccgcc gggatcactc tcggcatgga
     3661 cgagctgtac aagtaatcta gagggcccgt ttaaacccgc tgatcagcct cgactgtgcc
     3721 ttctagttgc cagccatctg ttgtttgccc ctcccccgtg ccttccttga ccctggaagg
     3781 tgccactccc actgtccttt cctaataaaa tgaggaaatt gcatcgcatt gtctgagtag
     3841 gtgtcattct attctggggg gtggggtggg gcaggacagc aagggggagg attgggaaga
     3901 caatagcagg catgctgggg atgcggtggg ctctatggct tctgaggcgg aaagaaccag
     3961 ctggggctct agggggtatc cccacgcgcc ctgtagcggc gcattaagcg cggcgggtgt
     4021 ggtggttacg cgcagcgtga ccgctacact tgccagcgcc ctagcgcccg ctcctttcgc
     4081 tttcttccct tcctttctcg ccacgttcgc cggctttccc cgtcaagctc taaatcgggg
     4141 gctcccttta gggttccgat ttagtgcttt acggcacctc gaccccaaaa aacttgatta
     4201 gggtgatggt tcacgtagtg ggccatcgcc ctgatagacg gtttttcgcc ctttgacgtt
     4261 ggagtccacg ttctttaata gtggactctt gttccaaact ggaacaacac tcaaccctat
     4321 ctcggtctat tcttttgatt tataagggat tttgccgatt tcggcctatt ggttaaaaaa
     4381 tgagctgatt taacaaaaat ttaacgcgaa ttaattctgt ggaatgtgtg tcagttaggg
     4441 tgtggaaagt ccccaggctc cccagcaggc agaagtatgc aaagcatgca tctcaattag
     4501 tcagcaacca ggtgtggaaa gtccccaggc tccccagcag gcagaagtat gcaaagcatg
     4561 catctcaatt agtcagcaac catagtcccg cccctaactc cgcccatccc gcccctaact
     4621 ccgcccagtt ccgcccattc tccgccccat ggctgactaa ttttttttat ttatgcagag
     4681 gccgaggccg cctctgcctc tgagctattc cagaagtagt gaggaggctt ttttggaggc
     4741 ctaggctttt gcaaaaagct cccgggagct tgtatatcca ttttcggatc tgatcaagag
     4801 acaggatgag gatcgtttcg catgattgaa caagatggat tgcacgcagg ttctccggcc
     4861 gcttgggtgg agaggctatt cggctatgac tgggcacaac agacaatcgg ctgctctgat
     4921 gccgccgtgt tccggctgtc agcgcagggg cgcccggttc tttttgtcaa gaccgacctg
     4981 tccggtgccc tgaatgaact gcaggacgag gcagcgcggc tatcgtggct ggccacgacg
     5041 ggcgttcctt gcgcagctgt gctcgacgtt gtcactgaag cgggaaggga ctggctgcta
     5101 ttgggcgaag tgccggggca ggatctcctg tcatctcacc ttgctcctgc cgagaaagta
     5161 tccatcatgg ctgatgcaat gcggcggctg catacgcttg atccggctac ctgcccattc
     5221 gaccaccaag cgaaacatcg catcgagcga gcacgtactc ggatggaagc cggtcttgtc
     5281 gatcaggatg atctggacga agagcatcag gggctcgcgc cagccgaact gttcgccagg
     5341 ctcaaggcgc gcatgcccga cggcgaggat ctcgtcgtga cccatggcga tgcctgcttg
     5401 ccgaatatca tggtggaaaa tggccgcttt tctggattca tcgactgtgg ccggctgggt
     5461 gtggcggacc gctatcagga catagcgttg gctacccgtg atattgctga agagcttggc
     5521 ggcgaatggg ctgaccgctt cctcgtgctt tacggtatcg ccgctcccga ttcgcagcgc
     5581 atcgccttct atcgccttct tgacgagttc ttctgagcgg gactctgggg ttcgaaatga
     5641 ccgaccaagc gacgcccaac ctgccatcac gagatttcga ttccaccgcc gccttctatg
     5701 aaaggttggg cttcggaatc gttttccggg acgccggctg gatgatcctc cagcgcgggg
     5761 atctcatgct ggagttcttc gcccacccca acttgtttat tgcagcttat aatggttaca
     5821 aataaagcaa tagcatcaca aatttcacaa ataaagcatt tttttcactg cattctagtt
     5881 gtggtttgtc caaactcatc aatgtatctt atcatgtctg tataccgtcg acctctagct
     5941 agagcttggc gtaatcatgg tcatagctgt ttcctgtgtg aaattgttat ccgctcacaa
     6001 ttccacacaa catacgagcc ggaagcataa agtgtaaagc ctggggtgcc taatgagtga
     6061 gctaactcac attaattgcg ttgcgctcac tgcccgcttt ccagtcggga aacctgtcgt
     6121 gccagctgca ttaatgaatc ggccaacgcg cggggagagg cggtttgcgt attgggcgct
     6181 cttccgcttc ctcgctcact gactcgctgc gctcggtcgt tcggctgcgg cgagcggtat
     6241 cagctcactc aaaggcggta atacggttat ccacagaatc aggggataac gcaggaaaga
     6301 acatgtgagc aaaaggccag caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt
     6361 ttttccatag gctccgcccc cctgacgagc atcacaaaaa tcgacgctca agtcagaggt
     6421 ggcgaaaccc gacaggacta taaagatacc aggcgtttcc ccctggaagc tccctcgtgc
     6481 gctctcctgt tccgaccctg ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa
     6541 gcgtggcgct ttctcatagc tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct
     6601 ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc ttatccggta
     6661 actatcgtct tgagtccaac ccggtaagac acgacttatc gccactggca gcagccactg
     6721 gtaacaggat tagcagagcg aggtatgtag gcggtgctac agagttcttg aagtggtggc
     6781 ctaactacgg ctacactaga agaacagtat ttggtatctg cgctctgctg aagccagtta
     6841 ccttcggaaa aagagttggt agctcttgat ccggcaaaca aaccaccgct ggtagcggtg
     6901 gtttttttgt ttgcaagcag cagattacgc gcagaaaaaa aggatctcaa gaagatcctt
     6961 tgatcttttc tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa gggattttgg
     7021 tcatgagatt atcaaaaagg atcttcacct agatcctttt aaattaaaaa tgaagtttta
     7081 aatcaatcta aagtatatat gagtaaactt ggtctgacag ttaccaatgc ttaatcagtg
     7141 aggcacctat ctcagcgatc tgtctatttc gttcatccat agttgcctga ctccccgtcg
     7201 tgtagataac tacgatacgg gagggcttac catctggccc cagtgctgca atgataccgc
     7261 gagacccacg ctcaccggct ccagatttat cagcaataaa ccagccagcc ggaagggccg
     7321 agcgcagaag tggtcctgca actttatccg cctccatcca gtctattaat tgttgccggg
     7381 aagctagagt aagtagttcg ccagttaata gtttgcgcaa cgttgttgcc attgctacag
     7441 gcatcgtggt gtcacgctcg tcgtttggta tggcttcatt cagctccggt tcccaacgat
     7501 caaggcgagt tacatgatcc cccatgttgt gcaaaaaagc ggttagctcc ttcggtcctc
     7561 cgatcgttgt cagaagtaag ttggccgcag tgttatcact catggttatg gcagcactgc
     7621 ataattctct tactgtcatg ccatccgtaa gatgcttttc tgtgactggt gagtactcaa
     7681 ccaagtcatt ctgagaatag tgtatgcggc gaccgagttg ctcttgcccg gcgtcaatac
     7741 gggataatac cgcgccacat agcagaactt taaaagtgct catcattgga aaacgttctt
     7801 cggggcgaaa actctcaagg atcttaccgc tgttgagatc cagttcgatg taacccactc
     7861 gtgcacccaa ctgatcttca gcatctttta ctttcaccag cgtttctggg tgagcaaaaa
     7921 caggaaggca aaatgccgca aaaaagggaa taagggcgac acggaaatgt tgaatactca
     7981 tactcttcct ttttcaatat tattgaagca tttatcaggg ttattgtctc atgagcggat
     8041 acatatttga atgtatttag aaaaataaac aaataggggt tccgcgcaca tttccccgaa
     8101 aagtgccacc tgacgtc
//
