LOCUS       sequence-496191-        6125 bp    DNA     circular SYN 05-JAN-2026
DEFINITION  Expresses BFP in a subset of cortical somatostatin neurons..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6125)
  AUTHORS   Green J, Bruno CA, Traunmuller L, Ding J, Hrvatin S, Wilson DE, 
            Khodadad T, Samuels J, Greenberg ME, Harvey CD
  TITLE     A cell-type-specific error-correction signal in the posterior 
            parietal cortex.
  JOURNAL   Nature. 2023 Aug;620(7973):366-373. doi: 10.1038/s41586-023-06357-1.
            Epub 2023 Jul 19.
  PUBMED    37468637
REFERENCE   2  (bases 1 to 6125)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Jan 5, 2026 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/s41586-023-06357-1"; journalName: "Nature"; date: 
            "2023-08"; volume: "620"; issue: "7973"; pages: "366-373"
COMMENT     Sequence Label: pAAV2-Sst44-mTagBFP2
FEATURES             Location/Qualifiers
     source          1..6125
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      449..1037
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     938..957
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     repeat_region   1099..1228
                     /label=AAV2 ITR (alternate)
                     /note="Functional equivalent of wild-type AAV2 ITR"
     polyA_signal    1410..1544
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     polyA_signal    1664..1798
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     2595..2619
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     regulatory      2704..2713
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             2716..2736
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             2743..2766
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             2773..3480
                     /codon_start=1
                     /product="enhanced monomeric blue fluorescent protein 
                     (Subach et al., 2011)"
                     /label=mTagBFP2
                     /note="mammalian codon-optimized"
                     /translation="VSKGEELIKENMHMKLYMEGTVDNHHFKCTSEGEGKPYEGTQTMR
                     IKVVEGGPLPFAFDILATSFLYGSKTFINHTQGIPDFFKQSFPEGFTWERVTTYEDGGV
                     LTATQDTSLQDGCLIYNVKIRGVNFTSNGPVMQKKTLGWEAFTETLYPADGGLEGRNDM
                     ALKLVGGSHLIANAKTTYRSKKPAKNLKMPGVYYVDYRLERIKEANNETYVEQHEVAVA
                     RYCDLPSKLGHKLN"
     CDS             3487..3507
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     misc_feature    3554..4142
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(3607..3627)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(4025..4036)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     polyA_signal    4326..4407
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     repeat_region   4486..4626
                     /label=AAV2 ITR
                     /note="inverted terminal repeat of adeno-associated virus 
                     serotype 2"
     repeat_region   4486..4615
                     /label=AAV2 ITR
     rep_origin      4701..5156
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(4788..4807)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     4998..5019
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(5173..5192)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     5292..5314
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(5352..5370)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        5438..5542
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(5543..5611,5612..6125,1..278)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(5761..5780)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
ORIGIN
        1 aactacttac tctagcttcc cggcaacaat taatagactg gatggaggcg gataaagttg
       61 caggaccact tctgcgctcg gcccttccgg ctggctggtt tattgctgat aaatctggag
      121 ccggtgagcg tgggtctcgc ggtatcattg cagcactggg gccagatggt aagccctccc
      181 gtatcgtagt tatctacacg acggggagtc aggcaactat ggatgaacga aatagacaga
      241 tcgctgagat aggtgcctca ctgattaagc attggtaact gtcagaccaa gtttactcat
      301 atatacttta gattgattta aaacttcatt tttaatttaa aaggatctag gtgaagatcc
      361 tttttgataa tctcatgacc aaaatccctt aacgtgagtt ttcgttccac tgagcgtcag
      421 accccgtaga aaagatcaaa ggatcttctt gagatccttt ttttctgcgc gtaatctgct
      481 gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg tttgccggat caagagctac
      541 caactctttt tccgaaggta actggcttca gcagagcgca gataccaaat actgttcttc
      601 tagtgtagcc gtagttaggc caccacttca agaactctgt agcaccgcct acatacctcg
      661 ctctgctaat cctgttacca gtggctgctg ccagtggcga taagtcgtgt cttaccgggt
      721 tggactcaag acgatagtta ccggataagg cgcagcggtc gggctgaacg gggggttcgt
      781 gcacacagcc cagcttggag cgaacgacct acaccgaact gagataccta cagcgtgagc
      841 tatgagaaag cgccacgctt cccgaaggga gaaaggcgga caggtatccg gtaagcggca
      901 gggtcggaac aggagagcgc acgagggagc ttccaggggg aaacgcctgg tatctttata
      961 gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg
     1021 ggcggagcct atggaaaaac gccagcaacg cggccttttt acggttcctg gccttttgct
     1081 ggccttttgc tcacatgtcc tgcaggcagc tgcgcgctcg ctcgctcact gaggccgccc
     1141 gggcgtcggg cgacctttgg tcgcccggcc tcagtgagcg agcgagcgcg cagagaggga
     1201 gtggccaact ccatcactag gggttcctgc ggccgcacgc gttcgcgatg aataaatgaa
     1261 agcttgcaga tctgcgactc tagaggatct gcgactctag aggatcataa tcagccatac
     1321 cacattttgt agaggtttta cttgctttaa aaaacctccc acacctcccc ctgaacctga
     1381 aacataaaat gaatgcaatt gttgttgtta acttgtttat tgcagcttat aatggttaca
     1441 aataaagcaa tagcatcaca aatttcacaa ataaagcatt tttttcactg cattctagtt
     1501 gtggtttgtc caaactcatc aatgtatctt atcatgtctg gatctgcgac tctagaggat
     1561 cataatcagc cataccacat ttgtagaggt tttacttgct ttaaaaaacc tcccacacct
     1621 ccccctgaac ctgaaacata aaatgaatgc aattgttgtt gttaacttgt ttattgcagc
     1681 ttataatggt tacaaataaa gcaatagcat cacaaatttc acaaataaag catttttttc
     1741 actgcattct agttgtggtt tgtccaaact catcaatgta tcttatcatg tctggatccc
     1801 catcaagctg atccggaacc cctagggttc agtacccaga cactcccata cccttattta
     1861 gaagaaataa atatcatcaa gtcataatat ccttgactga ttaagaaagc cactttgtaa
     1921 gtgtttatta aactgtcaag aaacttacag aatttactac atgatcgtta gaataacttt
     1981 gagtcaggac atatttgata tgacttaatc atactccctc caaaaggaaa taaggctttg
     2041 tgaaggtaaa ttatttcttc ctgggttgga tatgtgttta tggagtgatc attcagctgt
     2101 tcccaacctt cattctgaaa aggcctcaga acacttcatg atgaatcaag ctgtatcctg
     2161 aatagagtaa aatgaaccac ttcgtaggaa ctatggtgtc accacatcag caattcttat
     2221 tgaaaagtgt gcatttctta ttcacatatt tcaaagatgg tattccagag gagtgatttt
     2281 ctcaatgtat ttttcatcta caagccttca ttttaagcct accaccgtgt gtgttttcaa
     2341 gacagcaatt atcgttttaa aatgtgcagg tctagcttga gcttctcagc aagtttctat
     2401 gccaaagaaa acaccaatcc tttccattta ctgagaatca atgtttaatc ctcctttttg
     2461 ttctcatact tattacaaat cataaagaat tctgagtgtc agtttgataa ctagaagctc
     2521 catgtaccat tcctgctcct tattgagtcg acgtataagc tttaggcgtg tacggtgggc
     2581 gcctataaaa gcagagctcg tttagtgaac cgtcagatcg cctggagcaa ttccacaaca
     2641 cttttgtctt ataccaactt tccgtaccac ttcctaccct cgtaaactct taagaaacta
     2701 gtagccacca tggtgccaaa gaagaagcgt aaggtaggat ccgactacaa ggacgacgac
     2761 gacaaaggat ccgtgtctaa gggcgaagag ctgattaagg agaacatgca catgaagctg
     2821 tacatggagg gcaccgtgga caaccatcac ttcaagtgca catccgaggg cgaaggcaag
     2881 ccctacgagg gcacccagac catgagaatc aaggtggtcg agggcggccc tctccccttc
     2941 gccttcgaca tcctggctac tagcttcctc tacggcagca agaccttcat caaccacacc
     3001 cagggcatcc ccgacttctt caagcagtcc ttccctgagg gcttcacatg ggagagagtc
     3061 accacatacg aagacggggg cgtgctgacc gctacccagg acaccagcct ccaggacggc
     3121 tgcctcatct acaacgtcaa gatcagaggg gtgaacttca catccaacgg ccctgtgatg
     3181 cagaagaaaa cactcggctg ggaggccttc accgagacgc tgtaccccgc tgacggcggc
     3241 ctggaaggca gaaacgacat ggccctgaag ctcgtgggcg ggagccatct gatcgcaaac
     3301 gccaagacca catatagatc caagaaaccc gctaagaacc tcaagatgcc tggcgtctac
     3361 tatgtggact acagactgga aagaatcaag gaggccaaca acgagaccta cgtcgagcag
     3421 cacgaggtgg cagtggccag atactgcgac ctccctagca aactggggca caagcttaat
     3481 ggaagcccca agaaaaagcg gaaggtgtaa ggcgcgccac ccctgcaggg aattcgatat
     3541 caagcttatc gataatcaac ctctggatta caaaatttgt gaaagattga ctggtattct
     3601 taactatgtt gctcctttta cgctatgtgg atacgctgct ttaatgcctt tgtatcatgc
     3661 tattgcttcc cgtatggctt tcattttctc ctccttgtat aaatcctggt tgctgtctct
     3721 ttatgaggag ttgtggcccg ttgtcaggca acgtggcgtg gtgtgcactg tgtttgctga
     3781 cgcaaccccc actggttggg gcattgccac cacctgtcag ctcctttccg ggactttcgc
     3841 tttccccctc cctattgcca cggcggaact catcgccgcc tgccttgccc gctgctggac
     3901 aggggctcgg ctgttgggca ctgacaattc cgtggtgttg tcggggaaat catcgtcctt
     3961 tccttggctg ctcgcctatg ttgccacctg gattctgcgc gggacgtcct tctgctacgt
     4021 cccttcggcc ctcaatccag cggaccttcc ttcccgcggc ctgctgccgg ctctgcggcc
     4081 tcttccgcgt cttcgccttc gccctcagac gagtcggatc tccctttggg ccgcctcccc
     4141 gcatcgatac cgagcgcgcg atcgcttccg cataatcgag agatctgtga tagcggccat
     4201 caagctggcc gcgactctag atcataatca gccataccac atttgtagag gttttacttg
     4261 ctttaaaaaa cctcccacac ctccccctga acctgaaaca taaaatgaat gcaattgttg
     4321 ttgttaactt gtttattgca gcttataatg gttacaaata aagcaatagc atcacaaatt
     4381 tcacaaataa agcatttttt tcactgcatt ctagttgtgg tttgtccaaa ctcatcaatg
     4441 tatcagctta tcgataccgc atgcacgtgc ggaccgagcg gccgcaggaa cccctagtga
     4501 tggagttggc cactccctct ctgcgcgctc gctcgctcac tgaggccggg cgaccaaagg
     4561 tcgcccgacg cccgggcttt gcccgggcgg cctcagtgag cgagcgagcg cgcagctgcc
     4621 tgcaggggcg cctgatgcgg tattttctcc ttacgcatct gtgcggtatt tcacaccgca
     4681 tacgtcaaag caaccatagt acgcgccctg tagcggcgca ttaagcgcgg cgggtgtggt
     4741 ggttacgcgc agcgtgaccg ctacacttgc cagcgcctta gcgcccgctc ctttcgcttt
     4801 cttcccttcc tttctcgcca cgttcgccgg ctttccccgt caagctctaa atcgggggct
     4861 ccctttaggg ttccgattta gtgctttacg gcacctcgac cccaaaaaac ttgatttggg
     4921 tgatggttca cgtagtgggc catcgccctg atagacggtt tttcgccctt tgacgttgga
     4981 gtccacgttc tttaatagtg gactcttgtt ccaaactgga acaacactca actctatctc
     5041 gggctattct tttgatttat aagggatttt gccgatttcg gtctattggt taaaaaatga
     5101 gctgatttaa caaaaattta acgcgaattt taacaaaata ttaacgttta caattttatg
     5161 gtgcactctc agtacaatct gctctgatgc cgcatagtta agccagcccc gacacccgcc
     5221 aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc
     5281 tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc
     5341 gagacgaaag ggcctcgtga tacgcctatt tttataggtt aatgtcatga taataatggt
     5401 ttcttagacg tcaggtggca cttttcgggg aaatgtgcgc ggaaccccta tttgtttatt
     5461 tttctaaata cattcaaata tgtatccgct catgagacaa taaccctgat aaatgcttca
     5521 ataatattga aaaaggaaga gtatgagtat tcaacatttc cgtgtcgccc ttattccctt
     5581 ttttgcggca ttttgccttc ctgtttttgc tcacccagaa acgctggtga aagtaaaaga
     5641 tgctgaagat cagttgggtg cacgagtggg ttacatcgaa ctggatctca acagcggtaa
     5701 gatccttgag agttttcgcc ccgaagaacg ttttccaatg atgagcactt ttaaagttct
     5761 gctatgtggc gcggtattat cccgtattga cgccgggcaa gagcaactcg gtcgccgcat
     5821 acactattct cagaatgact tggttgagta ctcaccagtc acagaaaagc atcttacgga
     5881 tggcatgaca gtaagagaat tatgcagtgc tgccataacc atgagtgata acactgcggc
     5941 caacttactt ctgacaacga tcggaggacc gaaggagcta accgcttttt tgcacaacat
     6001 gggggatcat gtaactcgcc ttgatcgttg ggaaccggag ctgaatgaag ccataccaaa
     6061 cgacgagcgt gacaccacga tgcctgtagc aatggcaaca acgttgcgca aactattaac
     6121 tggcg
//
