LOCUS       sequence-496217-        9486 bp    DNA     circular SYN 06-JAN-2026
DEFINITION  Golden Gate destination vector containing S. pyogenes Cas9 nuclease 
            (with BsaI sites removed). For multicassette gRNA assembly and 
            expression in moss Physcomitrium patens (Physcomitrella)..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9486)
  AUTHORS   Kozgunova E
  TITLE     tRNA-Based Polycistronic CRISPR/Cas9 System Boosts Efficiency of 
            Multi-Gene Deletion in the Moss Physcomitrella
  JOURNAL   bioRxiv 2025.07.29.667574
REFERENCE   2  (bases 1 to 9486)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Jan 6, 2026 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "bioRxiv 
            2025.07.29.667574"
COMMENT     Sequence Label: EKv444(pIhcsrI677-Cas9 GG destination vector )
FEATURES             Location/Qualifiers
     source          1..9486
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     91..108
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(267..294)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(386..472)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     669..691
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     683..700
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     684..700
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     CDS             1434..5537
                     /codon_start=1
                     /product="Cas9 (Csn1) endonuclease from the Streptococcus 
                     pyogenes Type II CRISPR/Cas system"
                     /label=Cas9
                     /note="generates RNA-guided double strand breaks in DNA"
                     /translation="MDKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKK
                     NLIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEES
                     FLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIK
                     FRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRL
                     ENLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQ
                     IGDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVR
                     QQLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLL
                     RKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARG
                     NSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEY
                     FTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFD
                     SVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEER
                     LKTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFM
                     QLIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGR
                     HKPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLY
                     LYYLQNGRDMYVDQELDINRLSDYDVDHIVPQFFLKDDSIDNKVLTRSDKNRGKSDNVP
                     SEEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKH
                     VAQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYL
                     NAVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKT
                     EITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSK
                     ESILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGI
                     TIMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNE
                     LALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILAD
                     ANLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVL
                     DATLIHQSITGLYETRIDLSQLGGD"
     CDS             5550..5570
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     promoter        5649..5996
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     primer_bind     5939..5959
                     /label=35S promoter
                     /note="CaMV 35S promoter, forward primer"
     CDS             6016..7041
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     polyA_signal    7083..7259
                     /label=CaMV poly(A) signal
                     /note="cauliflower mosaic virus polyadenylation signal"
     primer_bind     complement(7751..7767)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7751..7767)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(7868..7887)
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     CDS             7879..8688
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP
                     DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA
                     FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD
                     FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD
                     RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     complement(7946..7965)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     rep_origin      8835..9423
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     9324..9343
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 tttcctgcgt tatcccctga ttctgtggat aaccgtatta ccgcctttga gtgagctgat
       61 accgctcgcc gcagccgaac gaccgagcgc agcgagtcag tgagcgagga agcggaagag
      121 cgcccaatac gcaaaccgcc tctccccgcg cgttggccga ttcattaatg cagctggcac
      181 gacaggtttc ccgactggaa agcgggcagt gagcgcaacg caattaatac gcgtaccgct
      241 agccaggaag agtttgtaga aacgcaaaaa ggccatccgt caggatggcc ttctgcttag
      301 tttgatgcct ggcagtttat ggcgggcgtc ctgcccgcca ccctccgggc cgttgcttca
      361 caacgttcaa atccgctccc ggcggatttg tcctactcag gagagcgttc accgacaaac
      421 aacagataaa acgaaaggcc cagtcttccg actgagcctt tcgttttatt tgatgcctgg
      481 cagttcccta ctctcgcgtt aacgctagca tggatgtttt cccagtcacg acgtcatcag
      541 gcgccattcg ccattcaggc tgcacaactg ttgggaaggg cgatcggtgc gggcctcttc
      601 gctattacgc cagctggcga aagggggatg tgctgcaagg cgattaagtt gggtaacgcc
      661 agggttttcc cagtcacgac gttgtaaaac gacggccagt gtttaaacgt gagttgagac
      721 cactagctgc aatccggtct ctccttgcgt ttaaacgtgt cgacgcttga agagaatgtg
      781 ggaacgagtt gcgtccgccg tgtcaggaag taattcgagc ccggggaaaa agtcggggac
      841 tgaagaatta ggtgctccag aaatagggtc cagatggcga gaatccatgg aattggcttc
      901 aggataagat cgagccgcac atacaatcac ttgtgggata gcgaaaagac ggagggaagg
      961 accgacgaaa ttgactagtt gggcgtgaga gactgacaat tcgacacaag tgatgacgta
     1021 aagataaact gaggcgcggt gcaaaattgg gtggacgagg ctgacattca tcatttcgcg
     1081 tgactgcatc catttttgaa aatcattgtg gtcaaaatgt cctaggatgt ctacatctgt
     1141 aacgccgcta gatctgcagc ctggattggg acacgatgag gcctacgcaa tgggaggttg
     1201 agaattagtt cagaggatcg atcatttatc atcacacaat cgggatgtag gattaattgc
     1261 gttgccacaa aagcgtggcg gggaatgtat cctagtccat catataatga cacagttagg
     1321 cttcttcagg cacacagatt cagttcactc gctctgcaac tttcctttcc acttggctcc
     1381 cgtatttcca atctcgagct tttgctagcg tttctctgaa ctgctttaga accatggaca
     1441 agaagtactc cattgggctc gatatcggca caaacagcgt cggctgggcc gtcattacgg
     1501 acgagtacaa ggtgccgagc aaaaaattca aagttctggg caataccgat cgccacagca
     1561 taaagaagaa cctcattggc gccctcctgt tcgactccgg ggagacggcc gaagccacgc
     1621 ggctcaaaag aacagcacgg cgcagatata cccgcagaaa gaatcggatc tgctacctgc
     1681 aggagatctt tagtaatgag atggctaagg tggatgactc tttcttccat aggctggagg
     1741 agtccttttt ggtggaggag gataaaaagc acgagcgcca cccaatcttt ggcaatatcg
     1801 tggacgaggt ggcgtaccat gaaaagtacc caaccatata tcatctgagg aagaagcttg
     1861 tagacagtac tgataaggct gacttgcggt tgatctatct cgcgctggcg catatgatca
     1921 aatttcgggg acacttcctc atcgaggggg acctgaaccc agacaacagc gatgtcgaca
     1981 aactctttat ccaactggtt cagacttaca atcagctttt cgaagagaac ccgatcaacg
     2041 catccggagt tgacgccaaa gcaatcctga gcgctaggct gtccaaatcc cggcggctcg
     2101 aaaacctcat cgcacagctc cctggggaga agaagaacgg cctgtttggt aatcttatcg
     2161 ccctgtcact cgggctgacc cccaacttta aatctaactt cgacctggcc gaagatgcca
     2221 agcttcaact gagcaaagac acctacgatg atgatctcga caatctgctg gcccagatcg
     2281 gcgaccagta cgcagacctt tttttggcgg caaagaacct gtcagacgcc attctgctga
     2341 gtgatattct gcgagtgaac acggagatca ccaaagctcc gctgagcgct agtatgatca
     2401 agcgctatga tgagcaccac caagacttga ctttgctgaa ggcccttgtc agacagcaac
     2461 tgcctgagaa gtacaaggaa attttcttcg atcagtctaa aaatggctac gccggataca
     2521 ttgacggcgg agcaagccag gaggaatttt acaaatttat taagcccatc ttggaaaaaa
     2581 tggacggcac cgaggagctg ctggtaaagc ttaacagaga agatctgttg cgcaaacagc
     2641 gcactttcga caatggaagc atcccccacc agattcacct gggcgaactg cacgctatcc
     2701 tcaggcggca agaggatttc tacccctttt tgaaagataa cagggaaaag attgagaaaa
     2761 tcctcacatt tcggataccc tactatgtag gccccctcgc ccggggaaat tccagattcg
     2821 cgtggatgac tcgcaaatca gaagaaacca tcactccctg gaacttcgag gaagtcgtgg
     2881 ataagggggc ctctgcccag tccttcatcg aaaggatgac taactttgat aaaaatctgc
     2941 ctaacgaaaa ggtgcttcct aaacactctc tgctgtacga gtacttcaca gtttataacg
     3001 agctcaccaa ggtcaaatac gtcacagaag ggatgagaaa gccagcattc ctgtctggag
     3061 agcagaagaa agctatcgtg gacctcctct tcaagacgaa ccggaaagtt accgtgaaac
     3121 agctcaaaga agactatttc aaaaagattg aatgtttcga ctctgttgaa atcagcggag
     3181 tggaggatcg cttcaacgca tccctgggaa cgtatcacga tctcctgaaa atcattaaag
     3241 acaaggactt cctggacaat gaggagaacg aggacattct tgaggacatt gtcctcaccc
     3301 ttacgttgtt tgaagatagg gagatgattg aagaacgctt gaaaacttac gctcatctct
     3361 tcgacgacaa agtcatgaaa cagctcaaga ggcgccgata tacaggatgg gggcggctgt
     3421 caagaaaact gatcaatggg atccgagaca agcagagtgg aaagacaatc ctggattttc
     3481 ttaagtccga tggatttgcc aaccggaact tcatgcagtt gatccatgat gactctctca
     3541 cctttaagga ggacatccag aaagcacaag tttctggcca gggggacagt cttcacgagc
     3601 acatcgctaa tcttgcaggt agcccagcta tcaaaaaggg aatactgcag accgttaagg
     3661 tcgtggatga actcgtcaaa gtaatgggaa ggcataagcc cgagaatatc gttatcgaga
     3721 tggcccgaga gaaccaaact acccagaagg gacagaagaa cagtagggaa aggatgaaga
     3781 ggattgaaga gggtataaaa gaactggggt cccaaatcct taaggaacac ccagttgaaa
     3841 acacccagct tcagaatgag aagctctacc tgtactacct gcagaacggc agggacatgt
     3901 acgtggatca ggaactggac atcaatcggc tctccgacta cgacgtggat catatcgtgc
     3961 cccagttttt tctcaaagat gattctattg ataataaagt gttgacaaga tccgataaaa
     4021 atagagggaa gagtgataac gtcccctcag aagaagttgt caagaaaatg aaaaattatt
     4081 ggcggcagct gctgaacgcc aaactgatca cacaacggaa gttcgataat ctgactaagg
     4141 ctgaacgagg tggcctgtct gagttggata aagccggctt catcaaaagg cagcttgttg
     4201 agacacgcca gatcaccaag cacgtggccc aaattctcga ttcacgcatg aacaccaagt
     4261 acgatgaaaa tgacaaactg attcgagagg tgaaagttat tactctgaag tctaagctgg
     4321 tatcagattt cagaaaggac tttcagtttt ataaggtgag agagatcaac aattaccacc
     4381 atgcgcatga tgcctacctg aatgcagtgg taggcactgc acttatcaaa aaatatccca
     4441 agcttgaatc tgaatttgtt tacggagact ataaagtgta cgatgttagg aaaatgatcg
     4501 caaagtctga gcaggaaata ggcaaggcca ccgctaagta cttcttttac agcaatatta
     4561 tgaatttttt caagaccgag attacactgg ccaatggaga gattcggaag cgaccactta
     4621 tcgaaacaaa cggagaaaca ggagaaatcg tgtgggacaa gggtagggat ttcgcgacag
     4681 tccggaaggt cctgtccatg ccgcaggtga acatcgttaa aaagaccgaa gtacagaccg
     4741 gaggcttctc caaggaaagt atcctcccga aaaggaacag cgacaagctg atcgcacgca
     4801 aaaaagattg ggaccccaag aaatacggcg gattcgattc tcctacagtc gcttacagtg
     4861 tactggttgt ggccaaagtg gagaaaggga agtctaaaaa actcaaaagc gtcaaggaac
     4921 tgctgggcat cacaatcatg gagcgatcaa gcttcgaaaa aaaccccatc gactttctcg
     4981 aggcgaaagg atataaagag gtcaaaaaag acctcatcat taagcttccc aagtactctc
     5041 tctttgagct tgaaaacggc cggaaacgaa tgctcgctag tgcgggcgag ctgcagaaag
     5101 gtaacgagct ggcactgccc tctaaatacg ttaatttctt gtatctggcc agccactatg
     5161 aaaagctcaa aggatctccc gaagataatg agcagaagca gctgttcgtg gaacaacaca
     5221 aacactacct tgatgagatc atcgagcaaa taagcgaatt ctccaaaaga gtgatcctcg
     5281 ccgacgctaa cctcgataag gtgctttctg cttacaataa gcacagggat aagcccatca
     5341 gggagcaggc agaaaacatt atccacttgt ttactctgac caacttgggc gcgcctgcag
     5401 ccttcaagta cttcgacacc accatagaca gaaagcggta cacctctaca aaggaggtcc
     5461 tggacgccac actgattcat cagtcaatta cggggctcta tgaaacaaga atcgacctct
     5521 ctcagctcgg tggagacagc agggctgacc ccaagaagaa gaggaaggtg tgagagctcg
     5581 aattcgtaat catgtctcga ctactccaaa aatgtcaaag atacagtctc agaagaccaa
     5641 agggctattg acacttttca acaaagggta tataatcggg aagactcctc ggattccatt
     5701 gcccagctat ctgtcacttc atcgaaagga cagtagaaaa ggaaggtggc tcctacaaat
     5761 gccatcattg cgataaagga aaggctatca ttcaagatgc ctctgccgac agtggtccca
     5821 aagatggacc cccacccacg aggagcatcg tggaaaaaga agacgttcca accacgtctt
     5881 caaagcaagt ggattgatgt gacatctcca ctgacgtaag ggatgacgca caatcccact
     5941 atccttcgca agacccttcc tctatataag gaagttcatt tcatttggag aggacagccc
     6001 aagcaaggag gtgatatgaa gaagcctgaa ctcaccgcga cgtctgtcga gaagtttctg
     6061 atcgaaaagt tcgacagcgt ctccgacctg atgcagctct cggagggcga agaatctcgt
     6121 gctttcagct tcgatgtagg agggcgtgga tatgtcctgc gggtaaatag ctgcgccgat
     6181 ggtttctaca aagatcgtta tgtttatcgg cactttgcat cggccgcgct cccgattccg
     6241 gaagtgcttg acattggaga gttcagcgag agcctgacct attgcatctc ccgccgtgca
     6301 cagggtgtca cgttgcaaga cctgcctgaa accgaactgc ccgctgttct tcagccggtc
     6361 gcggaggcta tggatgcgat cgctgcggcc gatcttagcc agacgagcgg gttcggccca
     6421 ttcggaccgc aaggaatcgg tcaatacact acatggcgtg atttcatatg cgcgattgct
     6481 gatccccatg tgtatcactg gcaaactgtg atggacgaca ccgtcagtgc gtccgtcgcg
     6541 caggctctcg atgagctgat gctttgggcc gaggattgcc ccgaagtccg gcacctcgtg
     6601 cacgcggatt tcggctccaa caatgtcctg acggacaatg gccgcataac agcggtcatt
     6661 gactggagcg aggcgatgtt cggggattcc caatacgagg tcgccaacat cttcttctgg
     6721 aggccgtggt tggcttgtat ggagcagcag acgcgctact tcgagcggag gcatccggag
     6781 cttgcaggat cgccgcggct ccgggcgtat atgctccgca ttggtcttga ccaactctat
     6841 cagagcttgg ttgacggcaa tttcgatgat gcagcttggg cgcagggtcg atgcgacgca
     6901 atcgtccgat ccggagccgg gactgtcggg cgtacacaaa tcgcccgcag aagcgcggcc
     6961 gtctggaccg atggctgtgt agaagtactc gccgatagtg gaaaccgacg ccccagcact
     7021 cgtcctaggg caaaggaata gtgaaatcac cagtctctct ctacaaatct atctctctct
     7081 attttctcca taaataatgt gtgagtagtt tcccgataag ggaaattagg gttcttatag
     7141 ggtttcgctc atgtgttgag catataagaa acccttagta tgtatttgta tttgtaaaat
     7201 acttctatca ataaaatttc taattcctaa aaccaaaatc cagtactaaa atccagatct
     7261 cctaaagtcc ctatagatct ttgtcgtgaa tataaaccag acacgagacg actaaacctg
     7321 gagcccagac gccgttcgaa gctagaagta ccgcttaggc aggaggccgt tagggaaaag
     7381 atgctaaggc agggttggtt acgttgactc ccccgtaggt ttggtttaaa tatgatgaag
     7441 tggacggaag gaaggaggaa gacaaggaag gataaggttg caggccctgt gcaaggtaag
     7501 aagatggaaa tttgatagag gtacgctact atacttatac tatacgctaa gggaatgctt
     7561 gtatttatac cctatacccc ctaataaccc cttatcaatt taagaaataa tccgcataag
     7621 cccccgctta aaaattggta tcagggccat gaataggtct atgaccaaaa ctcaagagga
     7681 taaaacctca ccaaaatacg aaagagttct taactctaaa gataaaagat ctttcaagat
     7741 caaaactgtc gtcatagctg tttcctgtgt gaaattgccg tgtctcaaaa tctctgatgt
     7801 tacattgcac aagataaaaa tatatcatca tgaacaataa aactgtctgc ttacataaac
     7861 agtaatacaa ggggtgttat gagccatatt caacgggaaa cgtcgaggcc gcgattaaat
     7921 tccaacatgg atgctgattt atatgggtat aaatgggctc gcgataatgt cgggcaatca
     7981 ggtgcgacaa tctatcgctt gtatgggaag cccgatgcgc cagagttgtt tctgaaacat
     8041 ggcaaaggta gcgttgccaa tgatgttaca gatgagatgg tcagactaaa ctggctgacg
     8101 gaatttatgc ctcttccgac catcaagcat tttatccgta ctcctgatga tgcatggtta
     8161 ctcaccactg cgatccccgg aaaaacagca ttccaggtat tagaagaata tcctgattca
     8221 ggtgaaaata ttgttgatgc gctggcagtg ttcctgcgcc ggttgcattc gattcctgtt
     8281 tgtaattgtc cttttaacag cgatcgcgta tttcgtctcg ctcaggcgca atcacgaatg
     8341 aataacggtt tggttgatgc gagtgatttt gatgacgagc gtaatggctg gcctgttgaa
     8401 caagtctgga aagaaatgca taaacttttg ccattctcac cggattcagt cgtcactcat
     8461 ggtgatttct cacttgataa ccttattttt gacgagggga aattaatagg ttgtattgat
     8521 gttggacgag tcggaatcgc agaccgatac caggatcttg ccatcctatg gaactgcctc
     8581 ggtgagtttt ctccttcatt acagaaacgg ctttttcaaa aatatggtat tgataatcct
     8641 gatatgaata aattgcagtt tcatttgatg ctcgatgagt ttttctaatc agaattggtt
     8701 aattggttgt aacactggca gagcattacg ctgacttgac gggacggcgc aagctcatga
     8761 ccaaaatccc ttaacgtgag ttacgcgtcg ttccactgag cgtcagaccc cgtagaaaag
     8821 atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa
     8881 aaaccaccgc taccagcggt ggtttgtttg ccggatcaag agctaccaac tctttttccg
     8941 aaggtaactg gcttcagcag agcgcagata ccaaatactg ttcttctagt gtagccgtag
     9001 ttaggccacc acttcaagaa ctctgtagca ccgcctacat acctcgctct gctaatcctg
     9061 ttaccagtgg ctgctgccag tggcgataag tcgtgtctta ccgggttgga ctcaagacga
     9121 tagttaccgg ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac acagcccagc
     9181 ttggagcgaa cgacctacac cgaactgaga tacctacagc gtgagctatg agaaagcgcc
     9241 acgcttcccg aagggagaaa ggcggacagg tatccggtaa gcggcagggt cggaacagga
     9301 gagcgcacga gggagcttcc agggggaaac gcctggtatc tttatagtcc tgtcgggttt
     9361 cgccacctct gacttgagcg tcgatttttg tgatgctcgt caggggggcg gagcctatgg
     9421 aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct tttgctggcc ttttgctcac
     9481 atgttc
//
