LOCUS       sequence-496219-       10947 bp    DNA     circular SYN 06-JAN-2026
DEFINITION  Express Flag-HA-RBM33 in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10947)
  AUTHORS   Thomas A, Rehfeld F, Zhang H, Chang TC, Goodarzi M, Gillet F, 
            Mendell JT
  TITLE     RBM33 directs the nuclear export of transcripts containing GC-rich 
            elements.
  JOURNAL   Genes Dev. 2022 May 1;36(9-10):550-565. doi: 10.1101/gad.349456.122.
            Epub 2022 May 19.
  PUBMED    35589130
REFERENCE   2  (bases 1 to 10947)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Jan 6, 2026 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Genes 
            Dev."; date: "2022-05-1"; volume: "36(9-10):550-565. doi"; pages: " 
            10.1101/gad.349456.122. Epub 2022 May 19"
COMMENT     Sequence Label: pLJM1-FH-RBM33
FEATURES             Location/Qualifiers
     source          1..10947
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        36..239
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     190..210
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     CDS             324..347
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             348..374
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             2277..2303
                     /codon_start=1
                     /product="9xHis affinity tag"
                     /label=9xHis
                     /translation="HQHHHHHHH"
     misc_feature    3935..4052
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        4101..4611
                     /label=hPGK promoter
                     /note="human phosphoglycerate kinase 1 promoter"
     primer_bind     4530..4548
                     /label=hPGK-F
                     /note="Human PGK promoter, forward primer"
     CDS             4633..5232
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(4633..4652)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     5129..5149
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     LTR             5360..5593
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    5665..5786
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5702..5721)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     5756..5775
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      5826..5961
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(5981..6000)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(5982..6000)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(6010..6027)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(6010..6026)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6019..6041)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      6168..6623
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(6255..6274)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     6465..6486
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        6649..6753
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(6754..6822,6823..7614)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(6972..6991)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      7785..8373
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     8274..8293
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     8527..8544
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    8661..8682
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(8697..8702,8703..8720,8721..8727)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8735..8751
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     8740..8762
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     8759..8775
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     8759..8775
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     8794..8814
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        8796..8814
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        8842..9068
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             9069..9249
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    9296..9421
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    9914..10147
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             10332..10376
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     primer_bind     10674..10694
                     /label=hU6-F
                     /note="Human U6 promoter, forward primer"
ORIGIN
        1 agtacatcta cgtattagtc atcgctatta ccatggtgat gcggttttgg cagtacatca
       61 atgggcgtgg atagcggttt gactcacggg gatttccaag tctccacccc attgacgtca
      121 atgggagttt gttttggcac caaaatcaac gggactttcc aaaatgtcgt aacaactccg
      181 ccccattgac gcaaatgggc ggtaggcgtg tacggtggga ggtctatata agcagagctg
      241 gtttagtgaa ccgtcagatc cgctagcgct accgggtaca agtccggact cagatctcga
      301 gctcaagctt cgaattcgcc atggactaca aggacgacga tgacaagtac ccttatgacg
      361 tgcccgatta cgctagcggc cgcgcggccg ccctgggagc gagcggagga gcaggcgccg
      421 gagatgatga ctttgaccag tttgataagc ctggcgcgga acggtcgtgg agaagaagag
      481 ctgctgatga ggactgggac agtgaacttg aagatgattt acttggagaa gatttgctat
      541 ctggcaaaaa gaatcagtcg gatttgtcag atgaagagct aaatgatgat cttttgcaga
      601 gtgataatga agatgaagaa aatttcagtt ctcagggtgt tacaattagt ctgaatgcta
      661 catctggcat ggttacatca tttgaactct ctgacaacac taacgaccaa tctggagaac
      721 aggaatctga gtatgaacaa gaacaaggag aggatgaact ggtttatcac aaatctgatg
      781 gatcagaatt gtatactcaa gagtacccag aagaaggaca gtatgaaggc cacgaagctg
      841 agttgacaga agaccaaata gaatatgtgg aagagccaga ggaggagcag ctttacactg
      901 atgaagtgtt agacatcgag atcaatgaac ctttagatga atttacagga ggtatggaaa
      961 cattggaact tcaaaaggac atcaaagaag aatcagatga agaagaagaa gatgatgaag
     1021 aatctggacg attacgtttc aaaactgaaa ggaaagaagg aacaattatt aggctctcag
     1081 atgtaactag agagagaagg aacattccag aaactttgga gctttcagca gaggccaagg
     1141 cagcattgct tgaatttgaa gaaagggagc gacagcataa acaaggacgc tacagctcca
     1201 ggcggggagg acggcgagga ggtccgctga tgtgtcgtgg tgtgggggac cagaggagag
     1261 agagcaccga gaggggcagg atgaaggacc acagacctgc gctgcttcct acacagcctc
     1321 ctgtcgtgcc ccaggctccc cctccaccgc caccgccgcc tcagcagcag ccgatcagaa
     1381 gcctgttcca gccgcagccg ctgcagccgc tgcttccggt gcagcacccg caccacccat
     1441 ccccgcctca gggaatgcac atgcctcccc agctagagac cccaaggatg atgatgaccc
     1501 cgccacccgt gactccacag cagcccaaga acatacacat caacccgcac ttcaaaggga
     1561 cggtggtcac gcctgttcaa gtgcccttgc taccagttcc gagccagccg agacctgccg
     1621 tgggacccca gagattccca ggccctccag aatttccaca gcatacacct ggacctgttc
     1681 ccaacagttt cagccagccc ccacgactcc ctctccagga ccagtggaga gccccacccc
     1741 cgcctcagga tcgagaccct ttcttcttag gagtttcagg tgaaccaaga ttcccgagcc
     1801 atctttttct ggaacagcga agtccccctc caccaccacc gcctcctacc cttcttaaca
     1861 gtagccatcc tgttcctact cagagtcctc taccattcac tcagccagga ccagcattta
     1921 atcagcaagg acagcagcca gtgttcccaa gagagcggcc cgtacgacca gccttgcagc
     1981 ctccaggtcc ggtggggatt ctgcacttta gccagcctgg gtcggcaacc acacggccct
     2041 tcattcctcc tagacagccg ttcctgccag gcccaggaca gccgtttctg cccacacaca
     2101 cacagcccaa cctgcagggg ccgttgcatc ctccattgcc ccctccgcat cagcctcagc
     2161 ctcagcaacc tcagcaacag cccccgccac agcaccagcc tccgcaccag cccccgcacc
     2221 agcccccgcc ccagcaccag cccccacccc agcacccacc acagcacccg ccgcagcacc
     2281 agcaccacca ccaccaccac cacctgtccg tcccgccccc tcctttgatg ccgatgtctc
     2341 agccacagtt ccggcctcac gtacagaccg ctcagcctca ggccagcagc agccggatgc
     2401 agtgccccca gcgccagggg ctccggcata atacaacttc tcagaatgta agcaagcggc
     2461 ccatgcagca aatgcagccc actgcgccaa ggaacagcaa tttgcgtgaa ttacccatag
     2521 cgccgtcaca cgtgatagaa atgagcagca gccgctgctc tgccacgccc tcagcacaag
     2581 tgaaacctat cgtcagcgcg tcaccaccct cgcgggccgt ggcgggttcc agaagctcac
     2641 agggaaagac ggaagtgaaa gtcaagccag ctagccctgt ggctcaacct aaagaagagg
     2701 caaaaacaga aacagagttt cctgatgaag atgaggaaac aaggttatat cgcttaaaga
     2761 tagaagaaca gaaacgccta agagaagaaa tcctgaaaca gaaggagtta cggcggcagc
     2821 agcaggctgg tgccaggaag aaggagctgc tggagagact cgcgcagcaa cagcagcagc
     2881 tgtacgctcc cccaccccca gcagagcagg aagagcaggc actgtcacca tcacccacca
     2941 acggtaaccc actgttgccc tttccaggtg cacaggtcag acaaaatgtg aagaacagac
     3001 ttcttgttaa aaaccaggat gtcagtattt caaacgttca gcccaaaaca tccaattttg
     3061 taccatccag tgccaacatg cagtatcaag gacaacagat gaaagcactg aaacatttga
     3121 gacagaccag aacagttcct caaagtcaga ctcagccgct gcataaagtg ctcccgatca
     3181 aacctgcaga tgtggaggag ccagctgtcc cccagactcc tcgagtggcg tccatccagg
     3241 gccggcccca ggacacaaag cctggcgtga aaaggactgt cacgcacagg acaaacagtg
     3301 gtggtggaga cgggccccac atcagctcca aggtcagggt gattaagctg tcaggtgggg
     3361 gaggagagag cgatggcttt tttcacccag aaggccagcc ccagcggctt ccccagcctc
     3421 cggaagtggg accacagcct gcccgcaagg tgacgctgac cagggggggc ctccagcagc
     3481 ccccacatct gccagcgggg ccccacgcac actcgcctgt ccctccaggg atcaaaagca
     3541 tccaaggaat tcacccggcg aagaaggcca tcatgcacgg acgaggcaga ggagtggccg
     3601 gtcccatggg ccgggggcgc ctgatgccaa acaagcagaa cctgcgggtg gtggagtgca
     3661 agccccagcc ctgcgtggtg tctgtggagg ggctgtcctc gtccaccacg gatgcccagc
     3721 tgaagagcct gctgatgtca gtgggaccca ttcagagttt acagatgctt cctcagcaac
     3781 ggaaagccat agctaagttc aaggagccag cccacgcatt agcatttcag cagaaattcc
     3841 acaggcatat gatagatttg tcccacataa atgtggccct gatcgtggag tgagaattct
     3901 cgacctcgag acaaatggca gtattcatcc acaattttaa aagaaaaggg gggattgggg
     3961 ggtacagtgc aggggaaaga atagtagaca taatagcaac agacatacaa actaaagaat
     4021 tacaaaaaca aattacaaaa attcaaaatt ttcgggttta ttacagggac agcagagatc
     4081 cactttggcc gcggctcgag ggggttgggg ttgcgccttt tccaaggcag ccctgggttt
     4141 gcgcagggac gcggctgctc tgggcgtggt tccgggaaac gcagcggcgc cgaccctggg
     4201 tctcgcacat tcttcacgtc cgttcgcagc gtcacccgga tcttcgccgc tacccttgtg
     4261 ggccccccgg cgacgcttcc tgctccgccc ctaagtcggg aaggttcctt gcggttcgcg
     4321 gcgtgccgga cgtgacaaac ggaagccgca cgtctcacta gtaccctcgc agacggacag
     4381 cgccagggag caatggcagc gcgccgaccg cgatgggctg tggccaatag cggctgctca
     4441 gcagggcgcg ccgagagcag cggccgggaa ggggcggtgc gggaggcggg gtgtggggcg
     4501 gtagtgtggg ccctgttcct gcccgcgcgg tgttccgcat tctgcaagcc tccggagcgc
     4561 acgtcggcag tcggctccct cgttgaccga atcaccgacc tctctcccca gggggatcca
     4621 ccggagctta ccatgaccga gtacaagccc acggtgcgcc tcgccacccg cgacgacgtc
     4681 cccagggccg tacgcaccct cgccgccgcg ttcgccgact accccgccac gcgccacacc
     4741 gtcgatccgg accgccacat cgagcgggtc accgagctgc aagaactctt cctcacgcgc
     4801 gtcgggctcg acatcggcaa ggtgtgggtc gcggacgacg gcgccgcggt ggcggtctgg
     4861 accacgccgg agagcgtcga agcgggggcg gtgttcgccg agatcggccc gcgcatggcc
     4921 gagttgagcg gttcccggct ggccgcgcag caacagatgg aaggcctcct ggcgccgcac
     4981 cggcccaagg agcccgcgtg gttcctggcc accgtcggcg tctcgcccga ccaccagggc
     5041 aagggtctgg gcagcgccgt cgtgctcccc ggagtggagg cggccgagcg cgccggggtg
     5101 cccgccttcc tggagacctc cgcgccccgc aacctcccct tctacgagcg gctcggcttc
     5161 accgtcaccg ccgacgtcga ggtgcccgaa ggaccgcgca cctggtgcat gacccgcaag
     5221 cccggtgcct gacgcccgcc ccacgacccg cagcgcccga ccgaaaggag cgcacgaccc
     5281 catgcatcgg tacctttaag accaatgact tacaaggcag ctgtagatct tagccacttt
     5341 ttaaaagaaa aggggggact ggaagggcta attcactccc aacgaagaca agatctgctt
     5401 tttgcttgta ctgggtctct ctggttagac cagatctgag cctgggagct ctctggctaa
     5461 ctagggaacc cactgcttaa gcctcaataa agcttgcctt gagtgcttca agtagtgtgt
     5521 gcccgtctgt tgtgtgactc tggtaactag agatccctca gaccctttta gtcagtgtgg
     5581 aaaatctcta gcagtagtag ttcatgtcat cttattattc agtatttata acttgcaaag
     5641 aaatgaatat cagagagtga gaggaacttg tttattgcag cttataatgg ttacaaataa
     5701 agcaatagca tcacaaattt cacaaataaa gcattttttt cactgcattc tagttgtggt
     5761 ttgtccaaac tcatcaatgt atcttatcat gtctggctct agctatcccg cccctaactc
     5821 cgcccatccc gcccctaact ccgcccagtt ccgcccattc tccgccccat ggctgactaa
     5881 ttttttttat ttatgcagag gccgaggccg cctcggcctc tgagctattc cagaagtagt
     5941 gaggaggctt ttttggaggc ctagggacgt acccaattcg ccctatagtg agtcgtatta
     6001 cgcgcgctca ctggccgtcg ttttacaacg tcgtgactgg gaaaaccctg gcgttaccca
     6061 acttaatcgc cttgcagcac atcccccttt cgccagctgg cgtaatagcg aagaggcccg
     6121 caccgatcgc ccttcccaac agttgcgcag cctgaatggc gaatgggacg cgccctgtag
     6181 cggcgcatta agcgcggcgg gtgtggtggt tacgcgcagc gtgaccgcta cacttgccag
     6241 cgccctagcg cccgctcctt tcgctttctt cccttccttt ctcgccacgt tcgccggctt
     6301 tccccgtcaa gctctaaatc gggggctccc tttagggttc cgatttagtg ctttacggca
     6361 cctcgacccc aaaaaacttg attagggtga tggttcacgt agtgggccat cgccctgata
     6421 gacggttttt cgccctttga cgttggagtc cacgttcttt aatagtggac tcttgttcca
     6481 aactggaaca acactcaacc ctatctcggt ctattctttt gatttataag ggattttgcc
     6541 gatttcggcc tattggttaa aaaatgagct gatttaacaa aaatttaacg cgaattttaa
     6601 caaaatatta acgcttacaa tttaggtggc acttttcggg gaaatgtgcg cggaacccct
     6661 atttgtttat ttttctaaat acattcaaat atgtatccgc tcatgagaca ataaccctga
     6721 taaatgcttc aataatattg aaaaaggaag agtatgagta ttcaacattt ccgtgtcgcc
     6781 cttattccct tttttgcggc attttgcctt cctgtttttg ctcacccaga aacgctggtg
     6841 aaagtaaaag atgctgaaga tcagttgggt gcacgagtgg gttacatcga actggatctc
     6901 aacagcggta agatccttga gagttttcgc cccgaagaac gttttccaat gatgagcact
     6961 tttaaagttc tgctatgtgg cgcggtatta tcccgtattg acgccgggca agagcaactc
     7021 ggtcgccgca tacactattc tcagaatgac ttggttgagt actcaccagt cacagaaaag
     7081 catcttacgg atggcatgac agtaagagaa ttatgcagtg ctgccataac catgagtgat
     7141 aacactgcgg ccaacttact tctgacaacg atcggaggac cgaaggagct aaccgctttt
     7201 ttgcacaaca tgggggatca tgtaactcgc cttgatcgtt gggaaccgga gctgaatgaa
     7261 gccataccaa acgacgagcg tgacaccacg atgcctgtag caatggcaac aacgttgcgc
     7321 aaactattaa ctggcgaact acttactcta gcttcccggc aacaattaat agactggatg
     7381 gaggcggata aagttgcagg accacttctg cgctcggccc ttccggctgg ctggtttatt
     7441 gctgataaat ctggagccgg tgagcgtggg tctcgcggta tcattgcagc actggggcca
     7501 gatggtaagc cctcccgtat cgtagttatc tacacgacgg ggagtcaggc aactatggat
     7561 gaacgaaata gacagatcgc tgagataggt gcctcactga ttaagcattg gtaactgtca
     7621 gaccaagttt actcatatat actttagatt gatttaaaac ttcattttta atttaaaagg
     7681 atctaggtga agatcctttt tgataatctc atgaccaaaa tcccttaacg tgagttttcg
     7741 ttccactgag cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga tccttttttt
     7801 ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg
     7861 ccggatcaag agctaccaac tctttttccg aaggtaactg gcttcagcag agcgcagata
     7921 ccaaatactg ttcttctagt gtagccgtag ttaggccacc acttcaagaa ctctgtagca
     7981 ccgcctacat acctcgctct gctaatcctg ttaccagtgg ctgctgccag tggcgataag
     8041 tcgtgtctta ccgggttgga ctcaagacga tagttaccgg ataaggcgca gcggtcgggc
     8101 tgaacggggg gttcgtgcac acagcccagc ttggagcgaa cgacctacac cgaactgaga
     8161 tacctacagc gtgagctatg agaaagcgcc acgcttcccg aagggagaaa ggcggacagg
     8221 tatccggtaa gcggcagggt cggaacagga gagcgcacga gggagcttcc agggggaaac
     8281 gcctggtatc tttatagtcc tgtcgggttt cgccacctct gacttgagcg tcgatttttg
     8341 tgatgctcgt caggggggca gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg
     8401 ttcctggcct tttgctggcc ttttgctcac atgttctttc ctgcgttatc ccctgattct
     8461 gtggataacc gtattaccgc ctttgagtga gctgataccg ctcgccgcag ccgaacgacc
     8521 gagcgcagcg agtcagtgag cgaggaagcg gaagagcgcc caatacgcaa accgcctctc
     8581 cccgcgcgtt ggccgattca ttaatgcagc tggcacgaca ggtttcccga ctggaaagcg
     8641 ggcagtgagc gcaacgcaat taatgtgagt tagctcactc attaggcacc ccaggcttta
     8701 cactttatgc ttccggctcg tatgttgtgt ggaattgtga gcggataaca atttcacaca
     8761 ggaaacagct atgaccatga ttacgccaag cgcgcaatta accctcacta aagggaacaa
     8821 aagctggagc tgcaagctta atgtagtctt atgcaatact cttgtagtct tgcaacatgg
     8881 taacgatgag ttagcaacat gccttacaag gagagaaaaa gcaccgtgca tgccgattgg
     8941 tggaagtaag gtggtacgat cgtgccttat taggaaggca acagacgggt ctgacatgga
     9001 ttggacgaac cactgaattg ccgcattgca gagatattgt atttaagtgc ctagctcgat
     9061 acataaacgg gtctctctgg ttagaccaga tctgagcctg ggagctctct ggctaactag
     9121 ggaacccact gcttaagcct caataaagct tgccttgagt gcttcaagta gtgtgtgccc
     9181 gtctgttgtg tgactctggt aactagagat ccctcagacc cttttagtca gtgtggaaaa
     9241 tctctagcag tggcgcccga acagggactt gaaagcgaaa gggaaaccag aggagctctc
     9301 tcgacgcagg actcggcttg ctgaagcgcg cacggcaaga ggcgaggggc ggcgactggt
     9361 gagtacgcca aaaattttga ctagcggagg ctagaaggag agagatgggt gcgagagcgt
     9421 cagtattaag cgggggagaa ttagatcgcg atgggaaaaa attcggttaa ggccaggggg
     9481 aaagaaaaaa tataaattaa aacatatagt atgggcaagc agggagctag aacgattcgc
     9541 agttaatcct ggcctgttag aaacatcaga aggctgtaga caaatactgg gacagctaca
     9601 accatccctt cagacaggat cagaagaact tagatcatta tataatacag tagcaaccct
     9661 ctattgtgtg catcaaagga tagagataaa agacaccaag gaagctttag acaagataga
     9721 ggaagagcaa aacaaaagta agaccaccgc acagcaagcg gccgctgatc ttcagacctg
     9781 gaggaggaga tatgagggac aattggagaa gtgaattata taaatataaa gtagtaaaaa
     9841 ttgaaccatt aggagtagca cccaccaagg caaagagaag agtggtgcag agagaaaaaa
     9901 gagcagtggg aataggagct ttgttccttg ggttcttggg agcagcagga agcactatgg
     9961 gcgcagcgtc aatgacgctg acggtacagg ccagacaatt attgtctggt atagtgcagc
    10021 agcagaacaa tttgctgagg gctattgagg cgcaacagca tctgttgcaa ctcacagtct
    10081 ggggcatcaa gcagctccag gcaagaatcc tggctgtgga aagataccta aaggatcaac
    10141 agctcctggg gatttggggt tgctctggaa aactcatttg caccactgct gtgccttgga
    10201 atgctagttg gagtaataaa tctctggaac agatttggaa tcacacgacc tggatggagt
    10261 gggacagaga aattaacaat tacacaagct taatacactc cttaattgaa gaatcgcaaa
    10321 accagcaaga aaagaatgaa caagaattat tggaattaga taaatgggca agtttgtgga
    10381 attggtttaa cataacaaat tggctgtggt atataaaatt attcataatg atagtaggag
    10441 gcttggtagg tttaagaata gtttttgctg tactttctat agtgaataga gttaggcagg
    10501 gatattcacc attatcgttt cagacccacc tcccaacccc gaggggaccc gacaggcccg
    10561 aaggaataga agaagaaggt ggagagagag acagagacag atccattcga ttagtgaacg
    10621 gatctcgacg gtatcgatca cgagactagc ctcgagcggc cgcccccttc accgagggcc
    10681 tatttcccat gattccttca tatttgcata tacgatacaa ggctgttaga gagataattg
    10741 gaattaattt gactgtaaac acaaagatat tagtacaaaa tacgtgacgt agaaagtaat
    10801 aatttcttgg gtagtttgca gttttaaaat tatgttttaa aatggactat catatgccaa
    10861 gtacgccccc tattgacgtc aatgacggta aatggcccgc ctggcattat gcccagtaca
    10921 tgaccttatg ggactttcct acttggc
//
