LOCUS       sequence-496233-       14617 bp    DNA     circular SYN 06-JAN-2026
DEFINITION  A Gateway-compatible vector harboring a cTurboID–myc fusion and 
            conferring hygromycin resistance in plants..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 14617)
  AUTHORS   Huang A, Zhang J, Liu Z, Schoen V, Verma D, Zheng H, Pedmale UV, 
            Dong J
  TITLE     Split-YFP-coupled interaction-dependent TurboID identifies new 
            functions of basal cell polarity in Arabidopsis.
  JOURNAL   Proc Natl Acad Sci U S A. 2025 Aug 12;122(32):e2502445122. doi: 
            10.1073/pnas.2502445122. Epub 2025 Aug 6.
  PUBMED    40768356
REFERENCE   2  (bases 1 to 14617)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Jan 6, 2026 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Proc Natl 
            Acad Sci U S A."; date: "2025-08-12"; volume: "122(32):e2502445122. 
            doi"; pages: " 10.1073/pnas.2502445122. Epub 2025 Aug 6"
COMMENT     Sequence Label: cTurboID-myc_pHygGate100
FEATURES             Location/Qualifiers
     source          1..14617
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    1..25
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     terminator      complement(111..363)
                     /label=MAS terminator
                     /note="mannopine synthase terminator"
     CDS             complement(759..1784)
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRSQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     K"
     promoter        complement(1958..2338)
                     /label=MAS promoter
                     /note="mannopine synthase promoter (Velten et al., 1984)"
     promoter        3334..3679
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     primer_bind     3622..3642
                     /label=35S promoter
                     /note="CaMV 35S promoter, forward primer"
     protein_bind    3691..3815
                     /gene="mutant version of attR"
                     /label=attR1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     promoter        join(3840..3845,3846..3863,3864..3870)
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an ""up"" mutation"
     CDS             3924..4583
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     complement(3990..4009)
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     CDS             4925..5230
                     /codon_start=1
                     /gene="ccdB"
                     /product="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /label=ccdB
                     /note="Plasmids containing the ccdB gene cannot be 
                     propagated in standard E. coli strains."
                     /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK
                     VSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     primer_bind     5109..5128
                     /label=ccdB-fwd
                     /note="ccdB gene, forward primer"
     protein_bind    complement(5271..5395)
                     /gene="mutant version of attR"
                     /label=attR2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     CDS             6139..6168
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     terminator      6188..6895
                     /label=OCS terminator
                     /note="octopine synthase terminator"
     primer_bind     complement(6920..6937)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(6920..6936)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6929..6951)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     misc_feature    7139..7163
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     CDS             8464..9093
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
     CDS             9522..10595
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="MSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
                     QAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
                     KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
                     GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
                     ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
                     LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
                     LVMRYRNLIEGEASAGS"
     rep_origin      10661..10855
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     primer_bind     complement(11091..11113)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     misc_feature    11199..11339
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     11254..11273
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     complement(11354..11371)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(11525..12113)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(11605..11624)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(13399..14193)
                     /codon_start=1
                     /gene="aphA-3"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM
                     TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED
                     EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
                     PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
                     FCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
ORIGIN
        1 tggcaggata tattgtggtg taaacaaatt gacgcttaga caacttaata acacattgcg
       61 gacgttttta atgtactgaa ttaacgccga attaattcga gctcggatct gataatttat
      121 ttgaaaattc ataagaaaag caaacgttac atgaattgat gaaacaatac aaagacagat
      181 aaagccacgc acatttagga tattggccga gattactgaa tattgagtaa gatcacggaa
      241 tttctgacag gagcatgtct tcaattcagc ccaaatggca gttgaaatac tcaaaccgcc
      301 ccatatgcag gagcggatca ttcattgttt gtttggttgc ctttgccaac atgggagtcc
      361 aagattctgc agtcaaatct cggtgacggg caggaccgga cggggcggta ccggcaggct
      421 gaagtccagc tgccagaaac ccacgtcatg ccagttcccg tgcttgaagc cggccgcccg
      481 cagcatgccg cggggggcat atccgagcgc ctcgtgcatg cgcacgctcg ggtcgttggg
      541 cagcccgatg acagcgacca cgctcttgaa gccctgtgcc tccagggact tcagcaggtg
      601 ggtgtagagc gtggagccca gtcccgtccg ctggtggcgg ggggagacgt acacggtcga
      661 ctcggccgtc cagtcgtagg cgttgcgtgc cttccagggg cccgcgtagg cgatgccggc
      721 gacctcgccg tccacctcgg cgacgagcca gggatagcct atttctttgc cctcggacga
      781 gtgctggggc gtcggtttcc actatcggcg agtacttcta cacagccatc ggtccagacg
      841 gccgcgcttc tgcgggcgat ttgtgtacgc ccgacagtcc cggctccgga tcggacgatt
      901 gcgtcgcatc gaccctgcgc ccaagctgca tcatcgaaat tgccgtcaac caagctctga
      961 tagagttggt caagaccaat gcggagcata tacgcccgga gtcgtggcga tcctgcaagc
     1021 tccggatgcc tccgctcgaa gtagcgcgtc tgctgctcca tacaagccaa ccacggcctc
     1081 cagaagaaga tgttggcgac ctcgtattgg gaatccccga acatcgcctc gctccagtca
     1141 atgaccgctg ttatgcggcc attgtccgtc aggacattgt tggagccgaa atccgcgtgc
     1201 acgaggtgcc ggacttcggg gcagtcctcg gcccaaagca tcagctcatc gagagcctgc
     1261 gcgacggacg cactgacggt gtcgtccatc acagtttgcc agtgatacac atggggatca
     1321 gcaatcgcgc atatgaaatc acgccatgta gtgtattgac cgattccttg cggtccgaat
     1381 gggccgaacc cgctcgtctg gctaagatcg gccgcagcga tcgcatccat agcctccgcg
     1441 accggttgta gaacagcggg cagttcggtt tcaggcaggt cttgcaacgt gacaccctgt
     1501 gaacggcggg agatgcaata ggtcaggctc tcgctaaact ccccaatgtc aagcacttcc
     1561 ggaatcggga gcgcggccga tgcaaagtgc cgataaacat aacgatcttt gtagaaacca
     1621 tcggcgcagc tatttacccg caggacatat ccacgccctc ctacatcgaa gctgaaagca
     1681 cgagattctt cgccctccga gagctgcatc aggtcggaga cgctgtcgaa cttttcgatc
     1741 agaaacttct cgacagacgt cgcggtgagt tcaggctttt tcatttgctc ccgcagacgg
     1801 acgaggtcgt ccgtccactc ctgcggttcc tgcggctcgg tacggaagtt gaccgtgctt
     1861 gtctcgatgt agtggttgac gatggtgcag accgccggca tgtccgcctc ggtggcacgg
     1921 cggatgtcgg ccgggcgtcg ttctgggctc atcgattcga tttggtgtat cgagattggt
     1981 tatgaaattc agatgctagt gtaatgtatt ggtaatttgg gaagatataa taggaagcaa
     2041 ggctatttat ccatttctga aaaggcgaaa tggcgtcacc gcgagcgtca cgcgcattcc
     2101 gttcttgctg taaagcgttg tttggtacac ttttgactag cgaggcttgg cgtgtcagcg
     2161 tatctattca aaagtcgtta atggctgcgg atcaagaaaa agttggaata gaaacagaat
     2221 acccgcgaaa ttcaggcccg gttgccatgt cctacacgcc gaaataaacg accaaattag
     2281 tagaaaaata aaaactgact cggatactta cgtcacgtct tgcgcactga tttgaaaaat
     2341 ctcagaattc caatcccaca aaaatctgag cttaacagca cagttgctcc tctcagagca
     2401 gaatcgggta ttcaacaccc tcatatcaac tactacgttg tgtataacgg tccacatgcc
     2461 ggtatatacg atgactgggg ttgtacaaag gcggcaacaa acggcgttcc cggagttgca
     2521 cacaagaaat ttgccactat tacagaggca agagcagcag ctgacgcgta cacaacaagt
     2581 cagcaaacag acaggttgaa cttcatcccc aaaggagaag ctcaactcaa gcccaagagc
     2641 tttgctaagg ccctaacaag cccaccaaag caaaaagccc actggctcac gctaggaacc
     2701 aaaaggccca gcagtgatcc agccccaaaa gagatctcct ttgccccgga gattacaatg
     2761 gacgatttcc tctatcttta cgatctagga aggaagttcg aaggtgaagg tgacgacact
     2821 atgttcacca ctgataatga gaaggttagc ctcttcaatt tcagaaagaa tgctgaccca
     2881 cagatggtta gagaggccta cgcagcaggt ctcatcaaga cgatctaccc gagtaacaat
     2941 ctccaggaga tcaaatacct tcccaagaag gttaaagatg cagtcaaaag attcaggact
     3001 aattgcatca agaacacaga gaaagacata tttctcaaga tcagaagtac tattccagta
     3061 tggacgattc aaggcttgct tcataaacca aggcaagtaa tagagattgg agtctctaaa
     3121 aaggtagttc ctactgaatc taaggccatg catggagtct aagattcaaa tcgaggatct
     3181 aacagaactc gccgtgaaga ctggcgaaca gttcatacag agtcttttac gactcaatga
     3241 caagaagaaa atcttcgtca acatggtgga gcacgacact ctggtctact ccaaaaatgt
     3301 caaagataca gtctcagaag accaaagggc tattgagact tttcaacaaa ggataatttc
     3361 gggaaacctc ctcggattcc attgcccagc tatctgtcac ttcatcgaaa ggacagtaga
     3421 aaaggaaggt ggctcctaca aatgccatca ttgcgataaa ggaaaggcta tcattcaaga
     3481 tctctctgcc gacagtggtc ccaaagatgg acccccaccc acgaggagca tcgtggaaaa
     3541 agaagacgtt ccaaccacgt cttcaaagca agtggattga tgtgacatct ccactgacgt
     3601 aagggatgac gcacaatccc actatccttc gcaagaccct tcctctatat aaggaagttc
     3661 atttcatttg gagaggacac gctcgagatc acaagtttgt acaaaaaagc tgaacgagaa
     3721 acgtaaaatg atataaatat caatatatta aattagattt tgcataaaaa acagactaca
     3781 taatactgta aaacacaaca tatccagtca ctatggcggc cgcattaggc accccaggct
     3841 ttacacttta tgcttccggc tcgtataatg tgtggatttt gagttaggat ccgtcgagat
     3901 tttcaggagc taaggaagct aaaatggaga aaaaaatcac tggatatacc accgttgata
     3961 tatcccaatg gcatcgtaaa gaacattttg aggcatttca gtcagttgct caatgtacct
     4021 ataaccagac cgttcagctg gatattacgg cctttttaaa gaccgtaaag aaaaataagc
     4081 acaagtttta tccggccttt attcacattc ttgcccgcct gatgaatgct catccggaat
     4141 tccgtatggc aatgaaagac ggtgagctgg tgatatggga tagtgttcac ccttgttaca
     4201 ccgttttcca tgagcaaact gaaacgtttt catcgctctg gagtgaatac cacgacgatt
     4261 tccggcagtt tctacacata tattcgcaag atgtggcgtg ttacggtgaa aacctggcct
     4321 atttccctaa agggtttatt gagaatatgt ttttcgtctc agccaatccc tgggtgagtt
     4381 tcaccagttt tgatttaaac gtggccaata tggacaactt cttcgccccc gttttcacca
     4441 tgggcaaata ttatacgcaa ggcgacaagg tgctgatgcc gctggcgatt caggttcatc
     4501 atgccgtttg tgatggcttc catgtcggca gaatgcttaa tgaattacaa cagtactgcg
     4561 atgagtggca gggcggggcg taaagatctg gatccggctt actaaaagcc agataacagt
     4621 atgcgtattt gcgcgctgat ttttgcggta taagaatata tactgatatg tatacccgaa
     4681 gtatgtcaaa aagaggtatg ctatgaagca gcgtattaca gtgacagttg acagcgacag
     4741 ctatcagttg ctcaaggcat atatgatgtc aatatctccg gtctggtaag cacaaccatg
     4801 cagaatgaag cccgtcgtct gcgtgccgaa cgctggaaag cggaaaatca ggaagggatg
     4861 gctgaggtcg cccggtttat tgaaatgaac ggctcttttg ctgacgagaa caggggctgg
     4921 tgaaatgcag tttaaggttt acacctataa aagagagagc cgttatcgtc tgtttgtgga
     4981 tgtacagagt gatattattg acacgcccgg gcgacggatg gtgatccccc tggccagtgc
     5041 acgtctgctg tcagataaag tctcccgtga actttacccg gtggtgcata tcggggatga
     5101 aagctggcgc atgatgacca ccgatatggc cagtgtgccg gtctccgtta tcggggaaga
     5161 agtggctgat ctcagccacc gcgaaaatga catcaaaaac gccattaacc tgatgttctg
     5221 gggaatataa atgtcaggct cccttataca cagccagtct gcaggtcgac catagtgact
     5281 ggatatgttg tgttttacag tattatgtag tctgtttttt atgcaaaatc taatttaata
     5341 tattgatatt tatatcattt tacgtttctc gttcagcttt cttgtacaaa gtggtgccta
     5401 ggtgagtcta gagggagcgt ggcagtcctg cctgtggtcg actccaccaa tcagtacctg
     5461 ctggatcgaa tcggcgagct gaagagtggg gatgcttgca ttgcagaata tcagcaggca
     5521 gggagaggaa gcagagggag gaaatggttc tctccttttg gagctaacct gtacctgagt
     5581 atgttttggc gcctgaagcg gggaccagca gcaatcggcc tgggcccggt catcggaatt
     5641 gtcatggcag aagcgctgcg aaagctggga gcagacaagg tgcgagtcaa atggcccaat
     5701 gacctgtatc tgcaggatag aaagctggca ggcatcctgg tggagctggc cggaataaca
     5761 ggcgatgctg cacagatcgt cattggcgcc gggattaacg tggctatgag gcgcgtggag
     5821 gaaagcgtgg tcaatcaggg ctggatcaca ctgcaggaag cagggattaa cctggacagg
     5881 aatactctgg ccgctacgct gatccgagag ctgcgggcag ccctggaact gttcgagcag
     5941 gaaggcctgg ctccatatct gccacggtgg gagaagctgg ataacttcat caatagaccc
     6001 gtgaagctga tcattgggga caaagagatt ttcgggatta gccgggggat tgataaacag
     6061 ggagccctgc tgctggaaca ggacggagtt atcaaaccct ggatgggcgg agaaatcagt
     6121 ctgcggtctg ccgaaaagga gcaaaagctc atttctgaag aggacttgta gactagtccc
     6181 tagagtcctg ctttaatgag atatgcgaga cgcctatgat cgcatgatat ttgctttcaa
     6241 ttctgttgtg cacgttgtaa aaaacctgag catgtgtagc tcagatcctt accgccggtt
     6301 tcggttcatt ctaatgaata tatcacccgt tactatcgta tttttatgaa taatattctc
     6361 cgttcaattt actgattgta ccctactact tatatgtaca atattaaaat gaaaacaata
     6421 tattgtgctg aataggttta tagcgacatc tatgatagag cgccacaata acaaacaatt
     6481 gcgttttatt attacaaatc caattttaaa aaaagcggca gaaccggtca aacctaaaag
     6541 actgattaca taaatcttat tcaaatttca aaagtgcccc aggggctagt atctacgaca
     6601 caccgagcgg cgaactaata acgctcactg aagggaactc cggttccccg ccggcgcgca
     6661 tgggtgagat tccttgaagt tgagtattgg ccgtccgctc taccgaaagt tacgggcacc
     6721 attcaacccg gtccagcacg gcggccgggt aaccgacttg ctgccccgag aattatgcag
     6781 catttttttg gtgtatgtgg gccccaaatg aagtgcaggt caaaccttga cagtgacgac
     6841 aaatcgttgg gcgggtccag ggcgaatttt gcgacaacat gtcgaggctc agcaggacct
     6901 gcaggcatgc aagcttggca ctggccgtcg ttttacaacg tcgtgactgg gaaaaccctg
     6961 gcgttaccca acttaatcgc cttgcagcac atcccccttt cgccagctgg cgtaatagcg
     7021 aagaggcccg caccgatcgc ccttcccaac agttgcgcag cctgaatggc gaatgctaga
     7081 gcagcttgag cttggatcag attgtcgttt cccgccttca gtttaaacta tcagtgtttg
     7141 acaggatata ttggcgggta aacctaagag aaaagagcgt ttattagaat aatcggatat
     7201 ttaaaagggc gtgaaaaggt ttatccgttc gtccatttgt atgtgcatgc caaccacagg
     7261 gttcccctcg ggatcaaagt actttgatcc aacccctccg ctgctatagt gcagtcggct
     7321 tctgacgttc agtgcagccg tcttctgaaa acgacatgtc gcacaagtcc taagttacgc
     7381 gacaggctgc cgccctgccc ttttcctggc gttttcttgt cgcgtgtttt agtcgcataa
     7441 agtagaatac ttgcgactag aaccggagac attacgccat gaacaagagc gccgccgctg
     7501 gcctgctggg ctatgcccgc gtcagcaccg acgaccagga cttgaccaac caacgggccg
     7561 aactgcacgc ggccggctgc accaagctgt tttccgagaa gatcaccggc accaggcgcg
     7621 accgcccgga gctggccagg atgcttgacc acctacgccc tggcgacgtt gtgacagtga
     7681 ccaggctaga ccgcctggcc cgcagcaccc gcgacctact ggacattgcc gagcgcatcc
     7741 aggaggccgg cgcgggcctg cgtagcctgg cagagccgtg ggccgacacc accacgccgg
     7801 ccggccgcat ggtgttgacc gtgttcgccg gcattgccga gttcgagcgt tccctaatca
     7861 tcgaccgcac ccggagcggg cgcgaggccg ccaaggcccg aggcgtgaag tttggccccc
     7921 gccctaccct caccccggca cagatcgcgc acgcccgcga gctgatcgac caggaaggcc
     7981 gcaccgtgaa agaggcggct gcactgcttg gcgtgcatcg ctcgaccctg taccgcgcac
     8041 ttgagcgcag cgaggaagtg acgcccaccg aggccaggcg gcgcggtgcc ttccgtgagg
     8101 acgcattgac cgaggccgac gccctggcgg ccgccgagaa tgaacgccaa gaggaacaag
     8161 catgaaaccg caccaggacg gccaggacga accgtttttc attaccgaag agatcgaggc
     8221 ggagatgatc gcggccgggt acgtgttcga gccgcccgcg cacgtctcaa ccgtgcggct
     8281 gcatgaaatc ctggccggtt tgtctgatgc caagctggcg gcctggccgg ccagcttggc
     8341 cgctgaagaa accgagcgcc gccgtctaaa aaggtgatgt gtatttgagt aaaacagctt
     8401 gcgtcatgcg gtcgctgcgt atatgatgcg atgagtaaat aaacaaatac gcaaggggaa
     8461 cgcatgaagg ttatcgctgt acttaaccag aaaggcgggt caggcaagac gaccatcgca
     8521 acccatctag cccgcgccct gcaactcgcc ggggccgatg ttctgttagt cgattccgat
     8581 ccccagggca gtgcccgcga ttgggcggcc gtgcgggaag atcaaccgct aaccgttgtc
     8641 ggcatcgacc gcccgacgat tgaccgcgac gtgaaggcca tcggccggcg cgacttcgta
     8701 gtgatcgacg gagcgcccca ggcggcggac ttggctgtgt ccgcgatcaa ggcagccgac
     8761 ttcgtgctga ttccggtgca gccaagccct tacgacatat gggccaccgc cgacctggtg
     8821 gagctggtta agcagcgcat tgaggtcacg gatggaaggc tacaagcggc ctttgtcgtg
     8881 tcgcgggcga tcaaaggcac gcgcatcggc ggtgaggttg ccgaggcgct ggccgggtac
     8941 gagctgccca ttcttgagtc ccgtatcacg cagcgcgtga gctacccagg cactgccgcc
     9001 gccggcacaa ccgttcttga atcagaaccc gagggcgacg ctgcccgcga ggtccaggcg
     9061 ctggccgctg aaattaaatc aaaactcatt tgagttaatg aggtaaagag aaaatgagca
     9121 aaagcacaaa cacgctaagt gccggccgtc cgagcgcacg cagcagcaag gctgcaacgt
     9181 tggccagcct ggcagacacg ccagccatga agcgggtcaa ctttcagttg ccggcggagg
     9241 atcacaccaa gctgaagatg tacgcggtac gccaaggcaa gaccattacc gagctgctat
     9301 ctgaatacat cgcgcagcta ccagagtaaa tgagcaaatg aataaatgag tagatgaatt
     9361 ttagcggcta aaggaggcgg catggaaaat caagaacaac caggcaccga cgccgtggaa
     9421 tgccccatgt gtggaggaac gggcggttgg ccaggcgtaa gcggctgggt tgcctgccgg
     9481 ccctgcaatg gcactggaac ccccaagccc gaggaatcgg cgtgagcggt cgcaaaccat
     9541 ccggcccggt acaaatcggc gcggcgctgg gtgatgacct ggtggagaag ttgaaggccg
     9601 cgcaggccgc ccagcggcaa cgcatcgagg cagaagcacg ccccggtgaa tcgtggcaag
     9661 cggccgctga tcgaatccgc aaagaatccc ggcaaccgcc ggcagccggt gcgccgtcga
     9721 ttaggaagcc gcccaagggc gacgagcaac cagatttttt cgttccgatg ctctatgacg
     9781 tgggcacccg cgatagtcgc agcatcatgg acgtggccgt tttccgtctg tcgaagcgtg
     9841 accgacgagc tggcgaggtg atccgctacg agcttccaga cgggcacgta gaggtttccg
     9901 cagggccggc cggcatggcc agtgtgtggg attacgacct ggtactgatg gcggtttccc
     9961 atctaaccga atccatgaac cgataccggg aagggaaggg agacaagccc ggccgcgtgt
    10021 tccgtccaca cgttgcggac gtactcaagt tctgccggcg agccgatggc ggaaagcaga
    10081 aagacgacct ggtagaaacc tgcattcggt taaacaccac gcacgttgcc atgcagcgta
    10141 cgaagaaggc caagaacggc cgcctggtga cggtatccga gggtgaagcc ttgattagcc
    10201 gctacaagat cgtaaagagc gaaaccgggc ggccggagta catcgagatc gagctagctg
    10261 attggatgta ccgcgagatc acagaaggca agaacccgga cgtgctgacg gttcaccccg
    10321 attacttttt gatcgatccc ggcatcggcc gttttctcta ccgcctggca cgccgcgccg
    10381 caggcaaggc agaagccaga tggttgttca agacgatcta cgaacgcagt ggcagcgccg
    10441 gagagttcaa gaagttctgt ttcaccgtgc gcaagctgat cgggtcaaat gacctgccgg
    10501 agtacgattt gaaggaggag gcggggcagg ctggcccgat cctagtcatg cgctaccgca
    10561 acctgatcga gggcgaagca tccgccggtt cctaatgtac ggagcagatg ctagggcaaa
    10621 ttgccctagc aggggaaaaa ggtcgaaaag gtctctttcc tgtggatagc acgtacattg
    10681 ggaacccaaa gccgtacatt gggaaccgga acccgtacat tgggaaccca aagccgtaca
    10741 ttgggaaccg gtcacacatg taagtgactg atataaaaga gaaaaaaggc gatttttccg
    10801 cctaaaactc tttaaaactt attaaaactc ttaaaacccg cctggcctgt gcataactgt
    10861 ctggccagcg cacagccgaa gagctgcaaa aagcgcctac ccttcggtcg ctgcgctccc
    10921 tacgccccgc cgcttcgcgt cggcctatcg cggccgctgg ccgctcaaaa atggctggcc
    10981 tacggccagg caatctacca gggcgcggac aagccgcgcc gtcgccactc gaccgccggc
    11041 gcccacatca aggcaccctg cctcgcgcgt ttcggtgatg acggtgaaaa cctctgacac
    11101 atgcagctcc cggagacggt cacagcttgt ctgtaagcgg atgccgggag cagacaagcc
    11161 cgtcagggcg cgtcagcggg tgttggcggg tgtcggggcg cagccatgac ccagtcacgt
    11221 agcgatagcg gagtgtatac tggcttaact atgcggcatc agagcagatt gtactgagag
    11281 tgcaccatat gcggtgtgaa ataccgcaca gatgcgtaag gagaaaatac cgcatcaggc
    11341 gctcttccgc ttcctcgctc actgactcgc tgcgctcggt cgttcggctg cggcgagcgg
    11401 tatcagctca ctcaaaggcg gtaatacggt tatccacaga atcaggggat aacgcaggaa
    11461 agaacatgtg agcaaaaggc cagcaaaagg ccaggaaccg taaaaaggcc gcgttgctgg
    11521 cgtttttcca taggctccgc ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga
    11581 ggtggcgaaa cccgacagga ctataaagat accaggcgtt tccccctgga agctccctcg
    11641 tgcgctctcc tgttccgacc ctgccgctta ccggatacct gtccgccttt ctcccttcgg
    11701 gaagcgtggc gctttctcat agctcacgct gtaggtatct cagttcggtg taggtcgttc
    11761 gctccaagct gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc gccttatccg
    11821 gtaactatcg tcttgagtcc aacccggtaa gacacgactt atcgccactg gcagcagcca
    11881 ctggtaacag gattagcaga gcgaggtatg taggcggtgc tacagagttc ttgaagtggt
    11941 ggcctaacta cggctacact agaaggacag tatttggtat ctgcgctctg ctgaagccag
    12001 ttaccttcgg aaaaagagtt ggtagctctt gatccggcaa acaaaccacc gctggtagcg
    12061 gtggtttttt tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct caagaagatc
    12121 ctttgatctt ttctacgggg tctgacgctc agtggaacga aaactcacgt taagggattt
    12181 tggtcatgca ttctagggaa ggtgcgaaca agtccctgat atgagatcat gtttgtcatc
    12241 tggagccata gaacagggtt catcatgagt catcaactta ccttcgccga cagtgaattc
    12301 agcagtaagc gccgtcagac cagaaaagag attttcttgt cccgcatgga gcagattctg
    12361 ccatggcaaa acatggtgga agtcatcgag ccgttttacc ccaaggctgg taatggccgg
    12421 cgaccttatc cgctggaaac catgctacgc attcactgca tgcagcattg gtacaacctg
    12481 agcgatggcg cgatggaaga tgctctgtac gaaatcgcct ccatgcgtct gtttgcccgg
    12541 ttatccctgg atagcgcctt gccggaccgc accaccatca tgaatttccg ccacctgctg
    12601 gagcagcatc aactggcccg ccaattgttc aagaccatca atcgctggct ggccgaagca
    12661 ggcgtcatga tgactcaagg caccttggtc gatgccacca tcattgaggc acccagctcg
    12721 accaagaaca aagagcagca acgcgatccg gagatgcatc agaccaagaa aggcaatcag
    12781 tggcactttg gcatgaaggc ccacattggt gtcgatgcca agagtggcct gacccacagc
    12841 ctggtcacca ccgcggccaa cgagcatgac ctcaatcagc tgggtaatct gctgcatgga
    12901 gaggagcaat ttgtctcagc cgatgccggc taccaagggg cgccacagcg cgaggagctg
    12961 gccgaggtgg atgtggactg gctgatcgcc gagcgccccg gcaaggtaag aaccttgaaa
    13021 cagcatccac gcaagaacaa aacggccatc aacatcgaat acatgaaagc cagcatccgg
    13081 gccagggtgg agcacccatt tcgcatcatc aagcgacagt tcggcttcgt gaaagccaga
    13141 tacaaggggt tgctgaaaaa cgataaccaa ctggcgatgt tattcacgct ggccaacctg
    13201 tttcgggcgg accaaatgat acgtcagtgg gagagatctc actaaaaact ggggataacg
    13261 ccttaaatgg cgaagaaacg gtctaaatag gctgattcaa ggcatttacg ggagaaaaaa
    13321 tcggctcaaa catgaagaaa tgaaatgact gagtcagccg agaagaattt ccccgcttat
    13381 tcgcaccttc cctaggtact aaaacaattc atccagtaaa atataatatt ttattttctc
    13441 ccaatcaggc ttgatcccca gtaagtcaaa aaatagctcg acatactgtt cttccccgat
    13501 atcctccctg atcgaccgga cgcagaaggc aatgtcatac cacttgtccg ccctgccgct
    13561 tctcccaaga tcaataaagc cacttacttt gccatctttc acaaagatgt tgctgtctcc
    13621 caggtcgccg tgggaaaaga caagttcctc ttcgggcttt tccgtcttta aaaaatcata
    13681 cagctcgcgc ggatctttaa atggagtgtc ttcttcccag ttttcgcaat ccacatcggc
    13741 cagatcgtta ttcagtaagt aatccaattc ggctaagcgg ctgtctaagc tattcgtata
    13801 gggacaatcc gatatgtcga tggagtgaaa gagcctgatg cactccgcat acagctcgat
    13861 aatcttttca gggctttgtt catcttcata ctcttccgag caaaggacgc catcggcctc
    13921 actcatgagc agattgctcc agccatcatg ccgttcaaag tgcaggacct ttggaacagg
    13981 cagctttcct tccagccata gcatcatgtc cttttcccgt tccacatcat aggtggtccc
    14041 tttataccgg ctgtccgtca tttttaaata taggttttca ttttctccca ccagcttata
    14101 taccttagca ggagacattc cttccgtatc ttttacgcag cggtattttt cgatcagttt
    14161 tttcaattcc ggtgatattc tcattttagc catttattat ttccttcctc ttttctacag
    14221 tatttaaaga taccccaaga agctaattat aacaagacga actccaattc actgttcctt
    14281 gcattctaaa accttaaata ccagaaaaca gctttttcaa agttgttttc aaagttggcg
    14341 tataacatag tatcgacgga gccgattttg aaaccgcggt gatcacaggc agcaacgctc
    14401 tgtcatcgtt acaatcaaca tgctaccctc cgcgagatca tccgtgtttc aaacccggca
    14461 gcttagttgc cgttcttccg aatagcatcg gtaacatgag caaagtctgc cgccttacaa
    14521 cggctctccc gctgacgccg tcccggactg atgggctgcc tgtatcgagt ggtgattttg
    14581 tgccgagctg ccggtcgggg agctgttggc tggctgg
//
