LOCUS       sequence-496264-       10647 bp    DNA     circular SYN 06-JAN-2026
DEFINITION  Expresses alpha-amanitin resistant EYFP tagged POLR2A in mammalian 
            cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10647)
  AUTHORS   Darzacq X, Shav-Tal Y, de Turris V, Brody Y, Shenoy SM, Phair RD, 
            Singer RH
  TITLE     In vivo dynamics of RNA polymerase II transcription.
  JOURNAL   Nat Struct Mol Biol. 2007 Sep;14(9):796-806. Epub 2007 Aug 5.
  PUBMED    17676063
REFERENCE   2  (bases 1 to 10647)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Jan 6, 2026 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat Struct 
            Mol Biol."; date: "2007-09"; volume: "14(9)"; pages: "796-806. Epub 
            2007 Aug 5"
COMMENT     Sequence Label: pYFP-RPB1aAmr
FEATURES             Location/Qualifiers
     source          1..10647
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      1..589
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     490..509
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     enhancer        764..1067
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        1068..1271
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     1222..1242
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     regulatory      1310..1319
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(1316..1318,1319..1321,1322..2032)
                     /codon_start=1
                     /product="enhanced YFP"
                     /label=EYFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTFGYGLQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSYQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(1622..1641)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     1969..1990
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     misc_feature    2033..2098
                     /label=MCS
                     /note="multiple cloning site"
     regulatory      2650..2659
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     regulatory      3053..3062
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     polyA_signal    8138..8259
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(8175..8194)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     8229..8248
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      complement(8266..8721)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(8403..8424)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     8615..8634
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     promoter        8748..8852
                     /gene="bla"
                     /label=AmpR promoter
     promoter        8854..9211
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     enhancer        8854..9045
                     /label=SV40 enhancer
                     /note="enhancer for the SV40 early promoter (Herr, 1993)"
     primer_bind     complement(8877..8897)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     rep_origin      9062..9197
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             9246..10040
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     CDS             9246..10040
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(9300..9319)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     9910..9929
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     complement(10228..10247)
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     polyA_signal    10272..10319
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
ORIGIN
        1 ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc
       61 agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt
      121 cagcagagcg cagataccaa atactgttct tctagtgtag ccgtagttag gccaccactt
      181 caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc
      241 tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa
      301 ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac
      361 ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg
      421 gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga
      481 gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact
      541 tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa
      601 cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc
      661 gttatcccct gattctgtgg ataaccgtat taccgccatg cattagttat taatagtaat
      721 caattacggg gtcattagtt catagcccat atatggagtt ccgcgttaca taacttacgg
      781 taaatggccc gcctggctga ccgcccaacg acccccgccc attgacgtca ataatgacgt
      841 atgttcccat agtaacgcca atagggactt tccattgacg tcaatgggtg gagtatttac
      901 ggtaaactgc ccacttggca gtacatcaag tgtatcatat gccaagtacg ccccctattg
      961 acgtcaatga cggtaaatgg cccgcctggc attatgccca gtacatgacc ttatgggact
     1021 ttcctacttg gcagtacatc tacgtattag tcatcgctat taccatggtg atgcggtttt
     1081 ggcagtacat caatgggcgt ggatagcggt ttgactcacg gggatttcca agtctccacc
     1141 ccattgacgt caatgggagt ttgttttggc accaaaatca acgggacttt ccaaaatgtc
     1201 gtaacaactc cgccccattg acgcaaatgg gcggtaggcg tgtacggtgg gaggtctata
     1261 taagcagagc tggtttagtg aaccgtcaga tccgctagcg ctaccggtcg ccaccatggt
     1321 gagcaagggc gaggagctgt tcaccggggt ggtgcccatc ctggtcgagc tggacggcga
     1381 cgtaaacggc cacaagttca gcgtgtccgg cgagggcgag ggcgatgcca cctacggcaa
     1441 gctgaccctg aagttcatct gcaccaccgg caagctgccc gtgccctggc ccaccctcgt
     1501 gaccaccttc ggctacggcc tgcagtgctt cgcccgctac cccgaccaca tgaagcagca
     1561 cgacttcttc aagtccgcca tgcccgaagg ctacgtccag gagcgcacca tcttcttcaa
     1621 ggacgacggc aactacaaga cccgcgccga ggtgaagttc gagggcgaca ccctggtgaa
     1681 ccgcatcgag ctgaagggca tcgacttcaa ggaggacggc aacatcctgg ggcacaagct
     1741 ggagtacaac tacaacagcc acaacgtcta tatcatggcc gacaagcaga agaacggcat
     1801 caaggtgaac ttcaagatcc gccacaacat cgaggacggc agcgtgcagc tcgccgacca
     1861 ctaccagcag aacaccccca tcggcgacgg ccccgtgctg ctgcccgaca accactacct
     1921 gagctaccag tccgccctga gcaaagaccc caacgagaag cgcgatcaca tggtcctgct
     1981 ggagttcgtg accgccgccg ggatcactct cggcatggac gagctgtaca agtccggact
     2041 cagatctcga gctcaagctt cgaattctgc agtcgacggt accgcgggcc cgggatccac
     2101 cggatctagc atgcacgggg gtggcccccc ctcgggggac agcgcatgcc cgctgcgcac
     2161 catcaagaga gtccagttcg gagtcctgag tccggatgaa ctgaagcgaa tgtctgtgac
     2221 ggagggtggc atcaaatacc cagagacgac tgagggaggc cgccccaagc ttggggggct
     2281 gatggacccg aggcaggggg tgattgagcg gactggccgc tgccaaacat gtgcaggaaa
     2341 catgacagag tgtcctggcc actttggcca cattgaactg gccaagcctg tgtttcacgt
     2401 gggcttcctg gtgaagacaa tgaaagtttt gcgctgtgtc tgcttcttct gctccaaact
     2461 gcttgtggac tctaacaacc caaagatcaa ggatatcctg gctaagtcca agggacagcc
     2521 caagaagcgg ctcacacatg tctacgacct ttgcaagggc aaaaacatat gcgagggtgg
     2581 ggaggagatg gacaacaagt tcggtgtgga acaacctgag ggtgacgagg atctgaccaa
     2641 agaaaagggc catggtggct gtgggcggta ccagcccagg atccggcgtt ctggcctaga
     2701 gctgtatgcg gaatggaagc acgttaatga ggactctcag gagaagaaga tcctgctgag
     2761 tccagagcga gtgcatgaga tcttcaaacg catctcagat gaggagtgtt ttgtgctggg
     2821 catggagccc cgctatgcac ggccagagtg gatgattgtc acagtgctgc ctgtgccccc
     2881 gctctccgtg cggcctgctg ttgtgatgca gggctctgcc cgtaaccagg atgacctgac
     2941 tcacaaactg gctgacatcg tgaagatcaa caatcagctg cggcgcaatg agcagaacgg
     3001 cgcagcggcc catgtcattg cagaggatgt gaagctcctc cagttccatg tggccaccat
     3061 ggtggacaat gagctgcctg gcttgccccg tgccatgcag aagtctgggc gtcccctcaa
     3121 gtccctgaag cagcggttga agggcaagga aggccgggtg cgagggaacc tgatgggcaa
     3181 aagagtggac ttctcggccc gtactgtcat cacccccgac cccaacctct ccattgacca
     3241 ggttggcgtg ccccgctcca ttgctgccaa catgaccttt gcggagattg tcaccccctt
     3301 caacattgac agacttcaag aactagtgcg cagggggaac agccagtacc caggcgccaa
     3361 gtacatcatc cgagacaatg gtgatcgcat tgacttgcgt ttccacccca agcccagtga
     3421 ccttcacctg cagaccggct ataaggtgga acggcacatg tgtgatgggg acattgttat
     3481 cttcaaccgg cagccaactc tgcacaaaat gtccatgatg gggcatcggg tccgcattct
     3541 cccatggtct acctttcgct tgaatcttag cgtgacaact ccgtacaatg cagactttga
     3601 cggggatgag atgaacttgc acctgccaca gtctctggag acgcgagcag agatccagga
     3661 gctggccatg gttcctcgca tgattgtcac cccccagagc aatcggcctg tcatgggtat
     3721 tgtgcaggac acactcacag cagtgcgcaa attcaccaag agagacgtct tcctggagcg
     3781 gggtgaagtg atgaacctcc tgatgttcct gtcgacgtgg gatgggaagg tcccacagcc
     3841 ggccatccta aagccccggc ccctgtggac aggcaagcaa atcttctccc tcatcatacc
     3901 tggtcacatc aattgtatcc gtacccacag cacccatccc gatgatgaag acagtggccc
     3961 ttacaagcac atctctcctg gggacaccaa ggtggtggtg gagaatgggg agctgatcat
     4021 gggcatcctg tgtaagaagt ctctgggcac gtcagctggc tccctggtcc acatctccta
     4081 cctagagatg ggtcatgaca tcactcgcct cttctactcc aacattcaga ctgtcattaa
     4141 caactggctc ctcatcgagg gtcatactat tggcattggg gactccattg ctgattctaa
     4201 gacttaccag gacattcaga acactattaa gaaggccaag caggacgtaa tagaggtcat
     4261 cgagaaggca cacaacaatg agctggagcc caccccaggg aacactctgc ggcagacgtt
     4321 tgagaatcag gtgaaccgca ttcttaacga tgcccgagac aagactggct cctctgctca
     4381 gaaatccctg tctgaataca acaacttcaa gtctatggtc gtgtccggag ctaaaggttc
     4441 caagattaac atctcccagg tcattgctgt cgttggacag caggacgtcg agggcaagcg
     4501 gattccattt ggcttcaagc accggactct gcctcacttc atcaaggatg actacgggcc
     4561 tgagagccgt ggctttgtgg agaactccta cctagccggc ctcacaccca ctgagttctt
     4621 tttccacgcc atggggggtc gtgaggggct cattgacacg gctgtcaaga ctgctgagac
     4681 tggatacatc cagcggcggc tgatcaagtc catggagtca gtgatggtga agtacgacgc
     4741 gactgtgcgg aactccatca accaggtggt gcagctgcgc tacggcgaag acggcctggc
     4801 aggcgagagc gttgagttcc agaacctggc tacgcttaag ccttccaaca aggcttttga
     4861 gaagaagttc cgctttgatt ataccaatga gagggccctg cggcgcactc tgcaggagga
     4921 cctggtgaag gacgtgctga gcaacgcaca catccagaac gagttggagc gggaatttga
     4981 gcggatgcgg gaggatcggg aggtgctcag ggtcatcttc ccaactggag acagcaaggt
     5041 cgtcctcccc tgtaacctgc tgcggatgat ctggaatgct cagaaaatct tccacatcaa
     5101 cccacgcctt ccctccgacc tgcaccccat caaagtggtg gagggagtca aggaattgag
     5161 caagaagctg gtgattgtga atggggatga cccactaagt cgacaggccc aggaaaatgc
     5221 cacgctgctc ttcaacatcc acctgcggtc cacgttgtgt tcccgccgca tggcagagga
     5281 gtttcggctc agtggggagg ccttcgactg gctgcttggg gagattgagt ccaagttcaa
     5341 ccaagccatt gcgcatcccg gggaaatggt gggggctctg gctgcgcagt cccttggaga
     5401 acctgccacc cagatgacct tgaatacctt ccactatgct ggtgtgtctg ccaagaatgt
     5461 gacgctgggt gtgccccgac ttaaggagct catcaacatt tccaagaagc caaagactcc
     5521 ttcgcttact gtcttcctgt tgggccagtc cgctcgagat gctgagagag ccaaggatat
     5581 tctgtgccgt ctggagcata caacgttgag gaaggtgact gccaacacag ccatctacta
     5641 tgaccccaac ccccagagca cggtggtggc agaggatcag gaatgggtga atgtctacta
     5701 tgaaatgcct gactttgatg tggcccgaat ctccccctgg ctgttgcggg tggagctgga
     5761 tcggaagcac atgactgacc ggaagctcac catggagcag attgctgaaa agatcaatgc
     5821 tggttttggt gacgacttga actgcatctt taatgatgac aatgcagaga agctggtgct
     5881 ccgtattcgc atcatgaaca gcgatgagaa caagatgcaa gaggaggaag aggtggtgga
     5941 caagatggat gatgatgtct tcctgcgctg catcgagtcc aacatgctga cagatatgac
     6001 cctgcagggc atcgagcaga tcagcaaggt gtacatgcac ttgccacaga cagacaacaa
     6061 gaagaagatc atcatcacgg aggatgggga attcaaggcc ctgcaggagt ggatcctgga
     6121 gacggacggc gtgagcttga tgcgggtgct gagtgagaag gacgtggacc ccgtacgcac
     6181 cacgtccaat gacattgtgg agatcttcac ggtgctgggc attgaagccg tgcggaaggc
     6241 cctggagcgg gagctgtacc acgtcatctc ctttgatggc tcctatgtca attaccgaca
     6301 cttggctctc ttgtgtgata ccatgacctg tcgtggccac ttgatggcca tcacccgaca
     6361 cggagtcaac cgccaggaca caggaccact catgaagtgt tcctttgagg aaacggtgga
     6421 cgtgcttatg gaagcagccg cacacggtga gagtgacccc atgaaggggg tctctgagaa
     6481 tatcatgctg ggccagctgg ctccggccgg cactggctgc tttgacctcc tgcttgatgc
     6541 agagaagtgc aagtatggca tggagatccc caccaatatc cccggcctgg gggctgctgg
     6601 acccaccggc atgttctttg gttcagcacc cagtcccatg ggtggaatct ctcctgccat
     6661 gacaccttgg aaccagggtg caacccctgc ctatggcgcc tggtccccca gtgttgggag
     6721 tggaatgacc ccaggggcag ccggcttctc tcccagtgct gcgtcagatg ccagcggctt
     6781 cagcccaggt tactcccctg cctggtctcc cacaccgggc tccccggggt ccccaggtcc
     6841 ctcaagcccc tacatccctt caccaggtgg tgccatgtct cccagctact cgccaacgtc
     6901 acctgcctac gagccccgct ctcctggggg ctacacaccc cagagtccct cttattcccc
     6961 cacttcaccc tcctactccc ctacctctcc atcctattct ccaaccagtc ccaactatag
     7021 tcccacatca cccagctatt cgccaacgtc acccagctac tcaccgacct ctcccagcta
     7081 ctcacccacc tctcccagct actcgcccac ctctcccagc tactcgccca cctctcccag
     7141 ctactcaccc acttccccta gctactcgcc cacttcccct agctactcgc caacgtctcc
     7201 cagctactcg ccgacatctc ccagctactc gccaacttca cccagctatt ctcccacttc
     7261 tcccagctac tcacctacct ctccaagcta ttcacccacc tcccccagct actcacccac
     7321 ttccccaagt tactcaccca ccagcccgaa ctattctcca accagtccca attacacccc
     7381 aacatcaccc agctacagcc cgacatcacc cagctattca cctactagtc ccaactacac
     7441 acctaccagc cctaactaca gcccaacctc tccaagctac tctccaacat cacccagcta
     7501 ttccccgacc tcaccaagtt actccccttc cagcccacga tacacaccac agtctccaac
     7561 ctatacccca agctcaccca gctacagccc cagctcgccc agctacagcc caacctcacc
     7621 caagtacacc ccaaccagtc cttcttacag tcccagctcc ccagagtata ccccaacctc
     7681 tcccaagtac tcacctacca gtcccaaata ttcacccacc tctcccaagt actcgcctac
     7741 cagtcccacc tattcaccca ccaccccaaa atactcccca acatctccta cttattcccc
     7801 aacctctcca gtctacaccc caacctctcc caagtactca cctactagcc ccacttactc
     7861 gcccacttcc cccaagtact cgcccaccag ccccacctac tcgcccacct cccccaaagg
     7921 ctcaacctac tctcccactt cccctggtta ctcgcccacc agccccacct acagtctcac
     7981 aagcccggct atcagcccgg atgacagtga cgaggagaac gctagataac tgatcataat
     8041 cagccatacc acatttgtag aggttttact tgctttaaaa aacctcccac acctccccct
     8101 gaacctgaaa cataaaatga atgcaattgt tgttgttaac ttgtttattg cagcttataa
     8161 tggttacaaa taaagcaata gcatcacaaa tttcacaaat aaagcatttt tttcactgca
     8221 ttctagttgt ggtttgtcca aactcatcaa tgtatcttaa ggcgtaaatt gtaagcgtta
     8281 atattttgtt aaaattcgcg ttaaattttt gttaaatcag ctcatttttt aaccaatagg
     8341 ccgaaatcgg caaaatccct tataaatcaa aagaatagac cgagataggg ttgagtgttg
     8401 ttccagtttg gaacaagagt ccactattaa agaacgtgga ctccaacgtc aaagggcgaa
     8461 aaaccgtcta tcagggcgat ggcccactac gtgaaccatc accctaatca agttttttgg
     8521 ggtcgaggtg ccgtaaagca ctaaatcgga accctaaagg gagcccccga tttagagctt
     8581 gacggggaaa gccggcgaac gtggcgagaa aggaagggaa gaaagcgaaa ggagcgggcg
     8641 ctagggcgct ggcaagtgta gcggtcacgc tgcgcgtaac caccacaccc gccgcgctta
     8701 atgcgccgct acagggcgcg tcaggtggca cttttcgggg aaatgtgcgc ggaaccccta
     8761 tttgtttatt tttctaaata cattcaaata tgtatccgct catgagacaa taaccctgat
     8821 aaatgcttca ataatattga aaaaggaaga gtcctgaggc ggaaagaacc agctgtggaa
     8881 tgtgtgtcag ttagggtgtg gaaagtcccc aggctcccca gcaggcagaa gtatgcaaag
     8941 catgcatctc aattagtcag caaccaggtg tggaaagtcc ccaggctccc cagcaggcag
     9001 aagtatgcaa agcatgcatc tcaattagtc agcaaccata gtcccgcccc taactccgcc
     9061 catcccgccc ctaactccgc ccagttccgc ccattctccg ccccatggct gactaatttt
     9121 ttttatttat gcagaggccg aggccgcctc ggcctctgag ctattccaga agtagtgagg
     9181 aggctttttt ggaggcctag gcttttgcaa agatcgatca agagacagga tgaggatcgt
     9241 ttcgcatgat tgaacaagat ggattgcacg caggttctcc ggccgcttgg gtggagaggc
     9301 tattcggcta tgactgggca caacagacaa tcggctgctc tgatgccgcc gtgttccggc
     9361 tgtcagcgca ggggcgcccg gttctttttg tcaagaccga cctgtccggt gccctgaatg
     9421 aactgcaaga cgaggcagcg cggctatcgt ggctggccac gacgggcgtt ccttgcgcag
     9481 ctgtgctcga cgttgtcact gaagcgggaa gggactggct gctattgggc gaagtgccgg
     9541 ggcaggatct cctgtcatct caccttgctc ctgccgagaa agtatccatc atggctgatg
     9601 caatgcggcg gctgcatacg cttgatccgg ctacctgccc attcgaccac caagcgaaac
     9661 atcgcatcga gcgagcacgt actcggatgg aagccggtct tgtcgatcag gatgatctgg
     9721 acgaagagca tcaggggctc gcgccagccg aactgttcgc caggctcaag gcgagcatgc
     9781 ccgacggcga ggatctcgtc gtgacccatg gcgatgcctg cttgccgaat atcatggtgg
     9841 aaaatggccg cttttctgga ttcatcgact gtggccggct gggtgtggcg gaccgctatc
     9901 aggacatagc gttggctacc cgtgatattg ctgaagagct tggcggcgaa tgggctgacc
     9961 gcttcctcgt gctttacggt atcgccgctc ccgattcgca gcgcatcgcc ttctatcgcc
    10021 ttcttgacga gttcttctga gcgggactct ggggttcgaa atgaccgacc aagcgacgcc
    10081 caacctgcca tcacgagatt tcgattccac cgccgccttc tatgaaaggt tgggcttcgg
    10141 aatcgttttc cgggacgccg gctggatgat cctccagcgc ggggatctca tgctggagtt
    10201 cttcgcccac cctaggggga ggctaactga aacacggaag gagacaatac cggaaggaac
    10261 ccgcgctatg acggcaataa aaagacagaa taaaacgcac ggtgttgggt cgtttgttca
    10321 taaacgcggg gttcggtccc agggctggca ctctgtcgat accccaccga gaccccattg
    10381 gggccaatac gcccgcgttt cttccttttc cccaccccac cccccaagtt cgggtgaagg
    10441 cccagggctc gcagccaacg tcggggcggc aggccctgcc atagcctcag gttactcata
    10501 tatactttag attgatttaa aacttcattt ttaatttaaa aggatctagg tgaagatcct
    10561 ttttgataat ctcatgacca aaatccctta acgtgagttt tcgttccact gagcgtcaga
    10621 ccccgtagaa aagatcaaag gatcttc
//
