LOCUS       sequence-49628-e         727 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 727)
  AUTHORS   Myeong-Je Chor, Wen Jiang, Peggy G. Lemaux
  TITLE     Transformation of recalcitrant barley cultivars through improvement 
            of regenerability and decreased albinism
  JOURNAL   Volume 138, Issue 2, 23 November 1998, Pages 229–244
REFERENCE   2  (bases 1 to 727)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Volume 138,
            Issue 2, 23 November 1998, Pages 229–244"
COMMENT     Sequence Label: pAct1IHPT-4
FEATURES             Location/Qualifiers
     source          1..727
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     terminator      408..660
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     primer_bind     complement(695..714)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(696..714)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
ORIGIN
        1 tcgtccgatc cggagccggg actgtcgggc gtacacaaat cgcccgcaga agcgcggccg
       61 tctggaccga tggctgtgta gaagtactcg ccgatagtgg aaaccgacgc cccagcactc
      121 gtccgagggc aaaggaatag agtagatgcc gaccgaacaa gagctgattt cgagaacgcc
      181 tcagccagca actcgcgcga gcctagcaag gcaaatgcga gagaacggcc ttacgcttgg
      241 tggcacagtt ctcgtccaca gttcgctaag ctcgctcggc tgggtcgcgg gagggccggt
      301 cgcagtgatt caggcccttc tggattgtgt tggtccccag ggcacgattg tccgggatct
      361 atcgattccc cctgaattcg agctcggtac cgagctcgaa tttccccgat cgttcaaaca
      421 tttggcaata aagtttctta agattgaatc ctgttgccgg tcttgcgatg attatcatat
      481 aatttctgtt gaattacgtt aagcatgtaa taattaacat gtaatgcatg acgttattta
      541 tgagatgggt ttttatgatt agagtcccgc aattatacat ttaatacgcg atagaaaaca
      601 aaatatagcg cgcaaactag gataaattat cgcgcgcggt gtcatctatg ttactagatc
      661 gggaattcat cgatgatatc agatctgccg gtctccctat agtgagtcgt attaatttcg
      721 ataagcc
//
