LOCUS       sequence-496312-       10951 bp    DNA     circular SYN 07-JAN-2026
DEFINITION  Expresses nucleus-localized NNG-Slug-nCas9 (D10A) fused to 
            PolI5M-Delta and mCherry using a CMV promoter. Includes a GFP 
            cassette flanked by BsmbI cut sites to knock in and coexpress 
            user-defined gRNAs..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10951)
  AUTHORS   Hurtado JE, Schieferecke AJ, Halperin SO, Guan J, Aidlen D, Schaffer
            DV, Dueber JE
  TITLE     Nickase fidelity drives EvolvR-mediated diversification in mammalian
            cells.
  JOURNAL   Nat Commun. 2025 Apr 19;16(1):3723. doi: 10.1038/s41467-025-58414-0.
  PUBMED    40253348
REFERENCE   2  (bases 1 to 10951)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Jan 7, 2026 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2025-04-19"; volume: "16(1):3723. doi"; pages: " 
            10.1038/s41467-025-58414-0"
COMMENT     Sequence Label: pEvolvR-mCherry-NNG-Slug-nCas9(D10A)-PolI5M-Delta
FEATURES             Location/Qualifiers
     source          1..10951
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      1..589
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     490..509
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     743..760
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    877..898
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(913..918,919..936,937..943)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    951..967
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     956..978
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     975..991
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     975..991
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     promoter        1012..1030
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        1153..1401
                     /label=U6 promoter
                     /note="RNA polymerase III promoter for human U6 snRNA 
                     (Domitrovich & Kunkel, 2003)"
     primer_bind     1153..1173
                     /label=hU6-F
                     /note="Human U6 promoter, forward primer"
     primer_bind     1324..1343
                     /label=LKO.1 5'
                     /note="Human U6 promoter, forward primer"
     RBS             1557..1568
                     /note="strong bacterial ribosome binding site (Elowitz and 
                     Leibler, 2000)"
     CDS             1575..2288
                     /codon_start=1
                     /product="GFP variant that folds robustly even when fused 
                     to poorly folded proteins (Pedelacq et al., 2006)"
                     /label=superfolder GFP
                     /translation="MRKGEELFTGVVPILVELDGDVNGHKFSVRGEGEGDATNGKLTLK
                     FICTTGKLPVPWPTLVTTLTYGVQCFARYPDHMKQHDFFKSAMPEGYVQERTISFKDDG
                     TYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNFNSHNVYITADKQKNGIKA
                     NFKIRHNVEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSVLSKDPNEKRDHMVLLE
                     FVTAAGITHGMDELYK"
     terminator      2300..2371
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     terminator      2387..2414
                     /label=T7Te terminator
                     /note="phage T7 early transcription terminator"
     enhancer        2534..2913
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        2914..3117
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     3068..3088
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     regulatory      3136..3145
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             3148..3168
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             3193..3897
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="VSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEGT
                     QTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNFE
                     DGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALKG
                     EIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERAE
                     GRHSTGGMDELYK"
     primer_bind     complement(3335..3353)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     3603..3622
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     CDS             7072..7098
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             7078..7104
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /note="This is a variant of the standard HA tag, which has 
                     sequence YPYDVPDYA. Some anti-HA antibodies may not 
                     recognize this variant."
                     /translation="YDVPDYASL"
     CDS             7117..7137
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     primer_bind     complement(9151..9170)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(9152..9170)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(9177..9194)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(9177..9193)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(9186..9208)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      9335..9790
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(9422..9441)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     9632..9653
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        9816..9920
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(9921..9989,9990..10781)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(10139..10158)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
ORIGIN
        1 ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc
       61 agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt
      121 cagcagagcg cagataccaa atactgttct tctagtgtag ccgtagttag gccaccactt
      181 caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc
      241 tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa
      301 ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac
      361 ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg
      421 gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga
      481 gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact
      541 tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa
      601 cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc
      661 gttatcccct gattctgtgg ataaccgtat taccgccttt gagtgagctg ataccgctcg
      721 cagcagccga acgaccgagc gcagcgagtc agtgagcgag gaagcggaag agcgcccaat
      781 acgcaaaccg cctctccccg cgcgttggcc gattcattaa tgcagctggc acgacaggtt
      841 tcccgactgg aaagcgggca gtgagcgcaa cgcaattaat gtgagttagc tcactcatta
      901 ggcaccccag gctttacact ttatgcttcc ggctcgtatg ttgtgtggaa ttgtgagcgg
      961 ataacaattt cacacaggaa acagctatga ccatgattac gccaagctcg aaattaaccc
     1021 tcactaaagg gaacaaaagc tggagctcca ccgcggtggc ggccgctctt aaggggtgca
     1081 gcggcctccg cgccgggttt tggcgcctcg gtgagtgagt gtgtgcgtgt tggatccaag
     1141 gtcgggcagg aagagggcct atttcccatg attccttcat atttgcatat acgatacaag
     1201 gctgttagag agataattag aattaatttg actgtaaaca caaagatatt agtacaaaat
     1261 acgtgacgta gaaagtaata atttcttggg tagtttgcag ttttaaaatt atgttttaaa
     1321 atggactatc atatgcttac cgtaacttga aagtatttcg atttcttggc tttatatatc
     1381 ttgtggaaag gacgaaacac ctgagacgga aagtgaaacg tgatttcatg cgtcattttg
     1441 aacattttgt aaatcttatt taataatgtg tgcggcaatt cacatttaat ttatgaatgt
     1501 tttcttaaca tcgcggcaac tcaagaaacg gcaggttcgg atcttagcta ctagagaaag
     1561 aggagaaata ctagatgcgt aaaggcgaag agctgttcac tggtgtcgtc cctattctgg
     1621 tggaactgga tggtgatgtc aacggtcata agttttccgt gcgtggcgag ggtgaaggtg
     1681 acgcaactaa tggtaaactg acgctgaagt tcatctgtac tactggtaaa ctgccggttc
     1741 cttggccgac tctggtaacg acgctgactt atggtgttca gtgctttgct cgttatccgg
     1801 accatatgaa gcagcatgac ttcttcaagt ccgccatgcc ggaaggctat gtgcaggaac
     1861 gcacgatttc ctttaaggat gacggcacgt acaaaacgcg tgcggaagtg aaatttgaag
     1921 gcgataccct ggtaaaccgc attgagctga aaggcattga ctttaaagag gacggcaata
     1981 tcctgggcca taagctggaa tacaatttta acagccacaa tgtttacatc accgccgata
     2041 aacaaaaaaa tggcattaaa gcgaatttta aaattcgcca caacgtggag gatggcagcg
     2101 tgcagctggc tgatcactac cagcaaaaca ctccaatcgg tgatggtcct gttctgctgc
     2161 cagacaatca ctatctgagc acgcaaagcg ttctgtctaa agatccgaac gagaaacgcg
     2221 atcatatggt tctgctggag ttcgtaaccg cagcgggcat cacgcatggt atggatgaac
     2281 tgtacaaatg accaggcatc aaataaaacg aaaggctcag tcgaaagact gggcctttcg
     2341 ttttatctgt tgtttgtcgg tgaacgctct ctactagagt cacactggct caccttcggg
     2401 tgggcctttc tgcgtttata cgtctcagtt ttagtactct ggaaacagaa tctactgaaa
     2461 caagacaata tgtcgtgttt atcccatcaa tttattggtg ggatttttcc gcggcctcta
     2521 gactcgaggc gttgacattg attattgact agttattaat agtaatcaat tacggggtca
     2581 ttagttcata gcccatatat ggagttccgc gttacataac ttacggtaaa tggcccgcct
     2641 ggctgaccgc ccaacgaccc ccgcccattg acgtcaataa tgacgtatgt tcccatagta
     2701 acgccaatag ggactttcca ttgacgtcaa tgggtggagt atttacggta aactgcccac
     2761 ttggcagtac atcaagtgta tcatatgcca agtacgcccc ctattgacgt caatgacggt
     2821 aaatggcccg cctggcatta tgcccagtac atgaccttat gggactttcc tacttggcag
     2881 tacatctacg tattagtcat cgctattacc atggtgatgc ggttttggca gtacatcaat
     2941 gggcgtggat agcggtttga ctcacgggga tttccaagtc tccaccccat tgacgtcaat
     3001 gggagtttgt tttggcacca aaatcaacgg gactttccaa aatgtcgtaa caactccgcc
     3061 ccattgacgc aaatgggcgg taggcgtgta cggtgggagg tctatataag cagagctctc
     3121 tggctaacta ccggtgccac catggcccca aagaagaagc ggaaggtcgg tatccacgga
     3181 gtcccagcag ccgtgagcaa gggcgaggag gataacatgg ccatcatcaa ggagttcatg
     3241 cgattcaagg tgcacatgga gggctccgtg aacggccacg agttcgagat cgagggcgag
     3301 ggcgagggcc gcccctacga gggcacccag accgccaagc tgaaggtgac caagggtggc
     3361 cccctgccct tcgcctggga catcctgtcc cctcagttca tgtacggctc caaggcctac
     3421 gtgaagcacc ccgccgacat ccccgactac ttgaagctgt ccttccccga gggcttcaag
     3481 tgggagcgcg tgatgaactt cgaggacggc ggcgtggtga ccgtgaccca ggactcctcc
     3541 ctgcaggacg gcgagttcat ctacaaggtg aagctgcgcg gcaccaactt cccctccgac
     3601 ggccccgtaa tgcagaagaa gaccatgggc tgggaggcct cctccgagcg gatgtacccc
     3661 gaggacggcg ccctgaaggg cgagatcaag cagaggctga agctgaagga cggcggccac
     3721 tacgacgctg aggtcaagac cacctacaag gccaagaagc ccgtgcagct gcccggcgcc
     3781 tacaacgtca acatcaagtt ggacatcacc tcccacaacg aggactacac catcgtggaa
     3841 cagtacgaac gcgccgaggg ccgccactcc accggcggca tggacgagct gtacaaggga
     3901 tccggtatga accaaaaatt catactggga ctggccatcg gaatcaccag cgtgggctac
     3961 ggcctgatcg actacgagac aaagaatatc atcgatgccg gcgttagact gttccccgag
     4021 gccaacgtgg aaaacaacga gggaagaagg tccaaacgtg gaagcagaag actgaagcga
     4081 cgccgcattc acagacttga acgggtgaag aagctgctcg aggattataa tctgctggat
     4141 cagtcccaga ttcctcagtc tacaaacccc tacgccatcc gcgtgaaggg cctgtctgaa
     4201 gccctgagca aggacgaact cgtgattgcc ctgctccata tcgccaagag aagaggcatc
     4261 cacaagatcg acgtgatcga cagcaacgac gacgtgggga acgagctcag caccaaggaa
     4321 cagctgaata agaacagcaa gctgctgaaa gacaaatttg tgtgccagat ccagctggaa
     4381 agaatgaatg agggccaggt gcggggagag aaaaaccggt tcaagaccgc tgatatcatc
     4441 aaggaaatca tccagctgct gaatgtgcag aagaacttcc accagctgga cgagaacttc
     4501 atcaacaagt acatcgaact ggttgagatg aggcgggaat acttcgaggg ccccggcaag
     4561 ggcagtccat atggctggga aggcgaccct aaggcttggt acgagacact gatgggccac
     4621 tgcacctact tcccagatga gctgagaagc gtgaaatacg cctacagtgc cgacctgttc
     4681 aacgctctga acgacctgaa caacctggtc atccaaagag atggactgtc taagctcgag
     4741 tatcatgaga agtatcacat catcgagaac gtgttcaagc agaagaagaa acctacactg
     4801 aagcagatcg ccaatgagat caatgtcaac cctgaagata tcaagggcta cagaatcaca
     4861 aagtctggca agccccagtt taccgagttt aagctctacc acgacctgaa aagcgtgctg
     4921 tttgaccaga gcatcctgga gaacgaagac gtgctggacc agatcgctga gatcctgacc
     4981 atctaccagg acaaggatag catcaaatct aagctgacgg aactggacat cctgctgaac
     5041 gaggaagata aggaaaacat cgcccagctg actggctaca ccgggaccca ccggctcagc
     5101 ctgaaatgca tccggctggt cctggaagag cagtggtatt ctagccggaa tcagatggaa
     5161 atcttcacac acctgaacat taagcctaag aagatcaacc tgacagccgc caacaagatc
     5221 ccgaaggcta tgatcgacga gttcatcctg agccctgtgg tgaagaggac cttcggccag
     5281 gccattaacc ttattaacaa gatcatagaa aagtacggcg tgcctgaaga tatcatcatc
     5341 gagctggcca gagaaaataa tagcaaggac aagcagaagt tcatcaatga gatgcagaaa
     5401 aagaacgaga acaccagaaa gagaattaac gaaatcatcg gcaagtatgg caaccagaac
     5461 gccaagagac tggtcgagaa gattagactg cacgacgagc aggagggcaa gtgcctgtac
     5521 tcactggaaa gcatccctct ggaggacctg ctgaacaacc ccaaccacta cgaggtggac
     5581 cacatcattc caagatctgt gtccttcgac aactcttacc acaacaaagt gctcgtgaag
     5641 cagagcgaga actccaaaaa atccaacctg accccttacc agtactttaa cagcggcaag
     5701 tccaagctct cttacaacca gtttaaacaa cacatcctga acctgagcaa gtcccaggat
     5761 agaatcagca aaaaaaagaa agagtatctg ctggaagaac gggacatcaa caagttcgag
     5821 gtgcaaaaag agttcatcaa tagaaacctg gtggataccc ggtacgccac aagagagctg
     5881 acaaactacc tgaaggccta cttcagcgcc aacaatatga acgtgaaggt gaaaacgatc
     5941 aacggcagct tcaccgatta cctgcggaaa gtgtggaagt ttaagaagga acggaaccac
     6001 ggctacaagc accacgccga ggacgccctg attatcgcta atgccgattt cctgttcaaa
     6061 gagaacaaga agctgaaagc cgtgaactct gtgctggaaa aacctgagat cgagagcaag
     6121 cagctggata tccaggtgga tagcgaggat aactacagcg aaatgttcat catccctaag
     6181 caggtccagg acatcaagga cttcagaaac ttcaagtaca gccacagagt ggacaagaag
     6241 cctaacagac ggctgatcaa cgatacactg tacagcaccc ggaagaagga caactccacc
     6301 tacatcgtgc agaccatcaa agatatctat gccaaagata ataccaccct gaagaagcag
     6361 tttgacaagt cacccgagaa gttcctcatg taccaacacg atccgcggac cttcgagaag
     6421 ttggaagtga tcatgaagca gtacgctaat gagaagaatc ctctggccaa gtaccacgag
     6481 gaaacaggcg agtacctgac caaatacagc aaaaaaaaca acggccctat cgtgaaaagc
     6541 ctgaagtaca ttggaaacaa gctgggccgc cacctagatg tgacccacca gttcaagagc
     6601 agcaccaaga agttggtgaa gcggagcatc aagccttata gattcgacgt ctacctgacc
     6661 gacaagggat ataagttcat caccatcagc tacctggacg tgctgaagaa agacaattac
     6721 tactacatac ccgaacagaa gtacgacaag ctcaaactgg gcaaggccat cgacaaaaac
     6781 gccaagttta tcgctagctt ctacaagaat gatctgatca agctggacgg cgagatctac
     6841 aagatcatcg gcgtgagtag cgacaccaga aacatgatcg aactggatct gcctgacatc
     6901 agatacaaag aatactgcga gctgaacaat atcaagggca aacctagaat cataaagacc
     6961 atcggcaaaa aggtgaatag catcgaaaaa ctgacaaccg acgtgctggg caacgtgttc
     7021 accaacaccc agtacacaaa acctcagctg ctgttcaagc gaggaaatgc ctatccctat
     7081 gacgtgcccg attatgccag cctgggcagc ggctccccca agaaaaaacg caaggtggaa
     7141 gatcctaaga aaaagcggaa aggttctagt gaaaccccgg gaacaagtga gtcggccacc
     7201 cctgaaggtg gatcaggggg tagcggatcc tcttacgata attatgttac gattctggac
     7261 gaggaaacct taaaggcttg gatcgctaaa ttagagaagg ctcctgtttt cgctttcgac
     7321 acggaaacgg attctctgga caatattagt gcgaatcttg ttggtctgag tttcgcaatt
     7381 gaaccgggtg ttgctgctta catccctgtg gcacacgact acctggacgc tccggaccag
     7441 atttcacgtg aacgcgctct ggaactgctg aagcctttat tagaggacga gaaagctttg
     7501 aaagttggtc agaatttgaa gtatgctcgt ggaatcttag ctaattatgg tatcgagttg
     7561 cgcggtatcg ctttcgacac gatgttggaa tcttatatcc tgaactctgt cgctggtcgc
     7621 catgacatgg actctctggc tgagcgctgg ctgaaacata agacgattac cttcgaggaa
     7681 atcgcaggaa agggtaagaa ccagctcacg ttcaatcaaa tcgctctgga ggaagctggt
     7741 cgctatgctg ctgaggacgc tgacgttact ctgcaactgc acttgaagat gtggcctgac
     7801 ttgcagaagc ataagggtcc actgaatgtt tttgaaaaca ttgagatgcc tttggttcca
     7861 gttctgtctc gtatcgagcg caatggcgtt aaaattgacc caaaggtttt acataaccac
     7921 tcagaggaac tgacgctgcg cttagccgaa ttggagaaaa aggctcacga gatcgctggc
     7981 gaagagttca atctgtcatc tacgaaacaa ctgcagacta tcctgttcga gaagcaaggt
     8041 atcaagccat taaaaaagac ccctggcggt gctccgtcta cctctgagga agttttggag
     8101 gagttagctt tggattaccc tctgccgaag gttatcttgg aataccgcgg tttggctaaa
     8161 ttgaagtcta cttatacgga taaacttcct ttgatgatta atccaaagac gggtcgcgtt
     8221 cacacgtcgt accatcaagc tgttaccgct accggtcgcc tgtcttctac ggatccgaat
     8281 ttacagaata ttcctgtgcg caatgaggag ggccgccgca ttcgtcaagc ttttatcgct
     8341 ccggaagact acgttatcgt ttctgctgat tattctcaaa atgaattacg tatcatggct
     8401 cacctgtctc gcgataaggg tctgttgacg gcctttgctg agggtaagga cattcatcgt
     8461 gctaccgctg ctgaggttta cggcctgccg ttggaaacgg ttacgtctga acagcgtcgc
     8521 tctgctaagc gtattaattt cggcttaatc tacggtatgt ctgcgtttgg cttagctcgt
     8581 cagctgaata tcccgcgcaa ggaagctcaa aaatatatgg atctgtattt tgagcgttac
     8641 cacggtgttt tggaatacat ggagcgtacg cgcgcgcaag ctaaggaaca aggttatgtg
     8701 gaaaccttgg atggtcgtcg cttgtacttg cctgacatta agtcttctaa cggcgcccgc
     8761 cgcgctgctg ccgagcgcgc tgctatcaat gctccgatgc aaggtactgc tgctgatatt
     8821 attaagcgtg ctatgatcgc tgtggacgct tggctgcaag ctgaacagcc tcgcgttcgc
     8881 atgattatgc aagttcatga cgagttggtt ttcgaggtgc ataaggacga cgtggacgct
     8941 gttgctaaac aaatccacca gttgatggag aattgcacgc gcttagacgt tccgctgctg
     9001 gttgaagttg gttctggtga aaactgggac caggctcact aatggctgaa aactgggacc
     9061 aggctcacta actcagatcc tactaggttt aataaacatc tttattttca ttacatctgt
     9121 gtgttggttt tttgtgtggt acccaattcg ccctatagtg agtcgtatta caattcactg
     9181 gccgtcgttt tacaacgtcg tgactgggaa aaccctggcg ttacccaact taatcgcctt
     9241 gcagcacatc cccctttcgc cagctggcgt aatagcgaag aggcccgcac cgatcgccct
     9301 tcccaacagt tgcgcagcct gaatggcgaa tgggacgcgc cctgtagcgg cgcattaagc
     9361 gcggcgggtg tggtggttac gcgcagcgtg accgctacac ttgccagcgc cctagcgccc
     9421 gctcctttcg ctttcttccc ttcctttctc gccacgttcg ccggctttcc ccgtcaagct
     9481 ctaaatcggg ggctcccttt agggttccga tttagtgctt tacggcacct cgaccccaaa
     9541 aaacttgatt agggtgatgg ttcacgtagt gggccatcgc cctgatagac ggtttttcgc
     9601 cctttgacgt tggagtccac gttctttaat agtggactct tgttccaaac tggaacaaca
     9661 ctcaacccta tctcggtcta ttcttttgat ttataaggga ttttgccgat ttcggcctat
     9721 tggttaaaaa atgagctgat ttaacaaaaa tttaacgcga attttaacaa aatattaacg
     9781 cttacaattt aggtggcact tttcggggaa atgtgcgcgg aacccctatt tgtttatttt
     9841 tctaaataca ttcaaatatg tatccgctca tgagacaata accctgataa atgcttcaat
     9901 aatattgaaa aaggaagagt atgagtattc aacatttccg tgtcgccctt attccctttt
     9961 ttgcggcatt ttgccttcct gtttttgctc acccagaaac gctggtgaaa gtaaaagatg
    10021 ctgaagatca gttgggtgca cgagtgggtt acatcgaact ggatctcaac agcggtaaga
    10081 tccttgagag ttttcgcccc gaagaacgtt ttccaatgat gagcactttt aaagttctgc
    10141 tatgtggcgc ggtattatcc cgtattgacg ccgggcaaga gcaactcggt cgccgcatac
    10201 actattctca gaatgacttg gttgagtact caccagtcac agaaaagcat cttacggatg
    10261 gcatgacagt aagagaatta tgcagtgctg ccataaccat gagtgataac actgcggcca
    10321 acttacttct gacaacgatc ggaggaccga aggagctaac cgcttttttg cacaacatgg
    10381 gggatcatgt aactcgcctt gatcgttggg aaccggagct gaatgaagcc ataccaaacg
    10441 acgagcgtga caccacgatg cctgtagcaa tggcaacaac gttgcgcaaa ctattaactg
    10501 gcgaactact tactctagct tcccggcaac aattaataga ctggatggag gcggataaag
    10561 ttgcaggacc acttctgcgc tcggcccttc cggctggctg gtttattgct gataaatctg
    10621 gagccggtga gcgtggttct cgcggtatca ttgcagcact ggggccagat ggtaagccct
    10681 cccgtatcgt agttatctac acgacgggga gtcaggcaac tatggatgaa cgaaatagac
    10741 agatcgctga gataggtgcc tcactgatta agcattggta actgtcagac caagtttact
    10801 catatatact ttagattgat ttaaaacttc atttttaatt taaaaggatc taggtgaaga
    10861 tcctttttga taatctcatg accaaaatcc cttaacgtga gttttcgttc cactgagcgt
    10921 cagaccccgt agaaaagatc aaaggatctt c
//
