LOCUS       sequence-496330-       12737 bp    DNA     circular SYN 07-JAN-2026
DEFINITION  PARP1 with split TurboID at C-term.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12737)
  TITLE     replication stress
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 12737)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Jan 7, 2026 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pLV-Hygro-EF1A-HA-PARP1-3GS-TurboC
FEATURES             Location/Qualifiers
     source          1..12737
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        3..229
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             230..410
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    457..582
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1075..1308
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1493..1537
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    1803..1920
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     protein_bind    1938..1958
                     /gene="mutant version of attB"
                     /label=attB4
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     promoter        1959..3137
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          2190..3128
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     3085..3105
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     protein_bind    3137..3161
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             3171..3197
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     protein_bind    complement(6984..7008)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     misc_feature    7026..7614
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(7079..7099)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(7497..7508)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     promoter        7645..8144
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(7680..7703)
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     primer_bind     8077..8096
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     CDS             8161..9186
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     CDS             8161..9186
                     /codon_start=1
                     /gene="aph(4)-Ia"
                     /product="aminoglycoside phosphotransferase from E. coli"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     LTR             9258..9491
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    9563..9684
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(9600..9619)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     9654..9673
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      9724..9859
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(9879..9898)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(9880..9898)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(9908..9925)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(9908..9924)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(9917..9939)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      10066..10521
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(10153..10172)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     10363..10384
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        10547..10651
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(10652..10720,10721..11512)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      11683..12271
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     12172..12191
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     12425..12442
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    12559..12580
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(12595..12600,12601..12618,12619..12625)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    12633..12649
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     12638..12660
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     12657..12673
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     12657..12673
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     12692..12712
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        12694..12712
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
ORIGIN
        1 aatgtagtct tatgcaatac tcttgtagtc ttgcaacatg gtaacgatga gttagcaaca
       61 tgccttacaa ggagagaaaa agcaccgtgc atgccgattg gtggaagtaa ggtggtacga
      121 tcgtgcctta ttaggaaggc aacagacggg tctgacatgg attggacgaa ccactgaatt
      181 gccgcattgc agagatattg tatttaagtg cctagctcga tacataaacg ggtctctctg
      241 gttagaccag atctgagcct gggagctctc tggctaacta gggaacccac tgcttaagcc
      301 tcaataaagc ttgccttgag tgcttcaagt agtgtgtgcc cgtctgttgt gtgactctgg
      361 taactagaga tccctcagac ccttttagtc agtgtggaaa atctctagca gtggcgcccg
      421 aacagggact tgaaagcgaa agggaaacca gaggagctct ctcgacgcag gactcggctt
      481 gctgaagcgc gcacggcaag aggcgagggg cggcgactgg tgagtacgcc aaaaattttg
      541 actagcggag gctagaagga gagagatggg tgcgagagcg tcagtattaa gcgggggaga
      601 attagatcgc gatgggaaaa aattcggtta aggccagggg gaaagaaaaa atataaatta
      661 aaacatatag tatgggcaag cagggagcta gaacgattcg cagttaatcc tggcctgtta
      721 gaaacatcag aaggctgtag acaaatactg ggacagctac aaccatccct tcagacagga
      781 tcagaagaac ttagatcatt atataataca gtagcaaccc tctattgtgt gcatcaaagg
      841 atagagataa aagacaccaa ggaagcttta gacaagatag aggaagagca aaacaaaagt
      901 aagaccaccg cacagcaagc ggccgctgat cttcagacct ggaggaggag atatgaggga
      961 caattggaga agtgaattat ataaatataa agtagtaaaa attgaaccat taggagtagc
     1021 acccaccaag gcaaagagaa gagtggtgca gagagaaaaa agagcagtgg gaataggagc
     1081 tttgttcctt gggttcttgg gagcagcagg aagcactatg ggcgcagcgt caatgacgct
     1141 gacggtacag gccagacaat tattgtctgg tatagtgcag cagcagaaca atttgctgag
     1201 ggctattgag gcgcaacagc atctgttgca actcacagtc tggggcatca agcagctcca
     1261 ggcaagaatc ctggctgtgg aaagatacct aaaggatcaa cagctcctgg ggatttgggg
     1321 ttgctctgga aaactcattt gcaccactgc tgtgccttgg aatgctagtt ggagtaataa
     1381 atctctggaa cagatttgga atcacacgac ctggatggag tgggacagag aaattaacaa
     1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga
     1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa
     1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat
     1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt
     1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg
     1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggtatcgcta
     1801 gcttttaaaa gaaaaggggg gattgggggg tacagtgcag gggaaagaat agtagacata
     1861 atagcaacag acatacaaac taaagaatta caaaaacaaa ttacaaaaat tcaaaatttt
     1921 actagtgatt atcggatcaa ctttgtatag aaaagttggg ctccggtgcc cgtcagtggg
     1981 cagagcgcac atcgcccaca gtccccgaga agttgggggg aggggtcggc aattgaaccg
     2041 gtgcctagag aaggtggcgc ggggtaaact gggaaagtga tgtcgtgtac tggctccgcc
     2101 tttttcccga gggtggggga gaaccgtata taagtgcagt agtcgccgtg aacgttcttt
     2161 ttcgcaacgg gtttgccgcc agaacacagg taagtgccgt gtgtggttcc cgcgggcctg
     2221 gcctctttac gggttatggc ccttgcgtgc cttgaattac ttccacctgg ctgcagtacg
     2281 tgattcttga tcccgagctt cgggttggaa gtgggtggga gagttcgagg ccttgcgctt
     2341 aaggagcccc ttcgcctcgt gcttgagttg aggcctggcc tgggcgctgg ggccgccgcg
     2401 tgcgaatctg gtggcacctt cgcgcctgtc tcgctgcttt cgataagtct ctagccattt
     2461 aaaatttttg atgacctgct gcgacgcttt ttttctggca agatagtctt gtaaatgcgg
     2521 gccaagatct gcacactggt atttcggttt ttggggccgc gggcggcgac ggggcccgtg
     2581 cgtcccagcg cacatgttcg gcgaggcggg gcctgcgagc gcggccaccg agaatcggac
     2641 gggggtagtc tcaagctggc cggcctgctc tggtgcctgg tctcgcgccg ccgtgtatcg
     2701 ccccgccctg ggcggcaagg ctggcccggt cggcaccagt tgcgtgagcg gaaagatggc
     2761 cgcttcccgg ccctgctgca gggagctcaa aatggaggac gcggcgctcg ggagagcggg
     2821 cgggtgagtc acccacacaa aggaaaaggg cctttccgtc ctcagccgtc gcttcatgtg
     2881 actccacgga gtaccgggcg ccgtccaggc acctcgatta gttctcgagc ttttggagta
     2941 cgtcgtcttt aggttggggg gaggggtttt atgcgatgga gtttccccac actgagtggg
     3001 tggagactga agttaggcca gcttggcact tgatgtaatt ctccttggaa tttgcccttt
     3061 ttgagtttgg atcttggttc attctcaagc ctcagacagt ggttcaaagt ttttttcttc
     3121 catttcaggt gtcgtgacaa gtttgtacaa aaaagcaggc tgccaccatg tacccatacg
     3181 atgttccaga ttacgctgcg gagtcttcgg ataagctcta tcgagtcgag tacgccaaga
     3241 gcgggcgcgc ctcttgcaag aaatgcagcg agagcatccc caaggactcg ctccggatgg
     3301 ccatcatggt gcagtcgccc atgtttgatg gaaaagtccc acactggtac cacttctcct
     3361 gcttctggaa ggtgggccac tccatccggc accctgacgt tgaggtggat gggttctctg
     3421 agcttcggtg ggatgaccag cagaaagtca agaagacagc ggaagctgga ggagtgacag
     3481 gcaaaggcca ggatggaatt ggtagcaagg cagagaagac tctgggtgac tttgcagcag
     3541 agtatgccaa gtccaacaga agtacgtgca aggggtgtat ggagaagata gaaaagggcc
     3601 aggtgcgcct gtccaagaag atggtggacc cggagaagcc acagctaggc atgattgacc
     3661 gctggtacca tccaggctgc tttgtcaaga acagggagga gctgggtttc cggcccgagt
     3721 acagtgcgag tcagctcaag ggcttcagcc tccttgctac agaggataaa gaagccctga
     3781 agaagcagct cccaggagtc aagagtgaag gaaagagaaa aggcgatgag gtggatggag
     3841 tggatgaagt ggcgaagaag aaatctaaaa aagaaaaaga caaggatagt aagcttgaaa
     3901 aagccctaaa ggctcagaac gacctgatct ggaacatcaa ggacgagcta aagaaagtgt
     3961 gttcaactaa tgacctgaag gagctactca tcttcaacaa gcagcaagtg ccttctgggg
     4021 agtcggcgat cttggaccga gtagctgatg gcatggtgtt cggtgccctc cttccctgcg
     4081 aggaatgctc gggtcagctg gtcttcaaga gcgatgccta ttactgcact ggggacgtca
     4141 ctgcctggac caagtgtatg gtcaagacac agacacccaa ccggaaggag tgggtaaccc
     4201 caaaggaatt ccgagaaatc tcttacctca agaaattgaa ggttaaaaaa caggaccgta
     4261 tattcccccc agaaaccagc gcctccgtgg cggccacgcc tccgccctcc acagcctcgg
     4321 ctcctgctgc tgtgaactcc tctgcttcag cagataagcc attatccaac atgaagatcc
     4381 tgactctcgg gaagctgtcc cggaacaagg atgaagtgaa ggccatgatt gagaaactcg
     4441 gggggaagtt gacggggacg gccaacaagg cttccctgtg catcagcacc aaaaaggagg
     4501 tggaaaagat gaataagaag atggaggaag taaaggaagc caacatccga gttgtgtctg
     4561 aggacttcct ccaggacgtc tccgcctcca ccaagagcct tcaggagttg ttcttagcgc
     4621 acatcttgtc cccttggggg gcagaggtga aggcagagcc tgttgaagtt gtggccccaa
     4681 gagggaagtc aggggctgcg ctctccaaaa aaagcaaggg ccaggtcaag gaggaaggta
     4741 tcaacaaatc tgaaaagaga atgaaattaa ctcttaaagg aggagcagct gtggatcctg
     4801 attctggact ggaacactct gcgcatgtcc tggagaaagg tgggaaggtc ttcagtgcca
     4861 cccttggcct ggtggacatc gttaaaggaa ccaactccta ctacaagctg cagcttctgg
     4921 aggacgacaa ggaaaacagg tattggatat tcaggtcctg gggccgtgtg ggtacggtga
     4981 tcggtagcaa caaactggaa cagatgccgt ccaaggagga tgccattgag cacttcatga
     5041 aattatatga agaaaaaacc gggaacgctt ggcactccaa aaatttcacg aagtatccca
     5101 aaaagttcta ccccctggag attgactatg gccaggatga agaggcagtg aagaagctga
     5161 cagtaaatcc tggcaccaag tccaagctcc ccaagccagt tcaggacctc atcaagatga
     5221 tctttgatgt ggaaagtatg aagaaagcca tggtggagta tgagatcgac cttcagaaga
     5281 tgcccttggg gaagctgagc aaaaggcaga tccaggccgc atactccatc ctcagtgagg
     5341 tccagcaggc ggtgtctcag ggcagcagcg actctcagat cctggatctc tcaaatcgct
     5401 tttacaccct gatcccccac gactttggga tgaagaagcc tccgctcctg aacaatgcag
     5461 acagtgtgca ggccaaggtg gaaatgcttg acaacctgct ggacatcgag gtggcctaca
     5521 gtctgctcag gggagggtct gatgatagca gcaaggatcc catcgatgtc aactatgaga
     5581 agctcaaaac tgacattaag gtggttgaca gagattctga agaagccgag atcatcagga
     5641 agtatgttaa gaacactcat gcaaccacac acaatgcgta tgacttggaa gtcatcgata
     5701 tctttaagat agagcgtgaa ggcgaatgcc agcgttacaa gccctttaag cagcttcata
     5761 accgaagatt gctgtggcac gggtccagga ccaccaactt tgctgggatc ctgtcccagg
     5821 gtcttcggat agccccgcct gaagcgcccg tgacaggcta catgtttggt aaagggatct
     5881 atttcgctga catggtctcc aagagtgcca actactgcca tacgtctcag ggagacccaa
     5941 taggcttaat cctgttggga gaagttgccc ttggaaacat gtatgaactg aagcacgctt
     6001 cacatatcag caagttaccc aagggcaagc acagtgtcaa aggtttgggc aaaactaccc
     6061 ctgatccttc agctaacatt agtctggatg gtgtagacgt tcctcttggg accgggattt
     6121 catctggtgt gaatgacacc tctctactat ataacgagta cattgtctat gatattgctc
     6181 aggtaaatct gaagtatctg ctgaaactga aattcaattt taagacctcc ctgtggggac
     6241 agctggacgg cgggagcgtg gcagtcctgc ctgtggtcga ctccaccaat cagtacctgc
     6301 tggatcgaat cggcgagctg aagagtgggg atgcttgcat tgcagaatat cagcaggcag
     6361 ggagaggaag cagagggagg aaatggttct ctccttttgg agctaacctg tacctgagta
     6421 tgttttggcg cctgaagcgg ggaccagcag caatcggcct gggcccggtc atcggaattg
     6481 tcatggcaga agcgctgcga aagctgggag cagacaaggt gcgagtcaaa tggcccaatg
     6541 acctgtatct gcaggataga aagctggcag gcatcctggt ggagctggcc ggaataacag
     6601 gcgatgctgc acagatcgtc attggcgccg ggattaacgt ggctatgagg cgcgtggagg
     6661 aaagcgtggt caatcagggc tggatcacac tgcaggaagc agggattaac ctggacagga
     6721 atactctggc cgctacgctg atccgagagc tgcgggcagc cctggaactg ttcgagcagg
     6781 aaggcctggc tccatatctg ccacggtggg agaagctgga taacttcatc aatagacccg
     6841 tgaagctgat cattggggac aaagagattt tcgggattag ccgggggatt gataaacagg
     6901 gagccctgct gctggaacag gacggagtta tcaaaccctg gatgggcgga gaaatcagtc
     6961 tgcggtctgc cgaaaaggct taaacccagc tttcttgtac aaagtggtga taatcgaatt
     7021 ccgataatca acctctggat tacaaaattt gtgaaagatt gactggtatt cttaactatg
     7081 ttgctccttt tacgctatgt ggatacgctg ctttaatgcc tttgtatcat gctattgctt
     7141 cccgtatggc tttcattttc tcctccttgt ataaatcctg gttgctgtct ctttatgagg
     7201 agttgtggcc cgttgtcagg caacgtggcg tggtgtgcac tgtgtttgct gacgcaaccc
     7261 ccactggttg gggcattgcc accacctgtc agctcctttc cgggactttc gctttccccc
     7321 tccctattgc cacggcggaa ctcatcgccg cctgccttgc ccgctgctgg acaggggctc
     7381 ggctgttggg cactgacaat tccgtggtgt tgtcggggaa gctgacgtcc tttccatggc
     7441 tgctcgcctg tgttgccacc tggattctgc gcgggacgtc cttctgctac gtcccttcgg
     7501 ccctcaatcc agcggacctt ccttcccgcg gcctgctgcc ggctctgcgg cctcttccgc
     7561 gtcttcgcct tcgccctcag acgagtcgga tctccctttg ggccgcctcc ccgcatcggg
     7621 aattcccgcg gttcgaattc taccgggtag gggaggcgct tttcccaagg cagtctggag
     7681 catgcgcttt agcagccccg ctgggcactt ggcgctacac aagtggcctc tggcctcgca
     7741 cacattccac atccaccggt aggcgccaac cggctccgtt ctttggtggc cccttcgcgc
     7801 caccttctac tcctccccta gtcaggaagt tcccccccgc cccgcagctc gcgtcgtgca
     7861 ggacgtgaca aatggaagta gcacgtctca ctagtctcgt gcagatggac agcaccgctg
     7921 agcaatggaa gcgggtaggc ctttggggca gcggccaata gcagctttgc tccttcgctt
     7981 tctgggctca gaggctggga aggggtgggt ccgggggcgg gctcaggggc gggctcaggg
     8041 gcggggcggg cgcccgaagg tcctccggag gcccggcatt ctgcacgctt caaaagcgca
     8101 cgtctgccgc gctgttctcc tcttcctcat ctccgggcct ttcgacctca cgtggccacc
     8161 atgaaaaagc ctgaactcac cgcgacgtct gtcgagaagt ttctgatcga aaagttcgac
     8221 agcgtctccg acctgatgca gctctcggag ggcgaagaat ctcgtgcttt cagcttcgat
     8281 gtaggagggc gtggatatgt cctgcgggta aatagctgcg ccgatggttt ctacaaagat
     8341 cgttatgttt atcggcactt tgcatcggcc gcgctcccga ttccggaagt gcttgacatt
     8401 ggggaattta gcgagagcct gacctattgc atctcccgcc gtgcacaggg tgtcacgttg
     8461 caagacctgc ctgaaaccga actgcccgct gttctgcagc cggtcgcgga ggccatggat
     8521 gcgatcgctg cggccgatct tagccagacg agcgggttcg gcccattcgg accgcaagga
     8581 atcggtcaat acactacatg gcgtgatttc atatgcgcga ttgctgatcc ccatgtgtat
     8641 cactggcaaa ctgtgatgga cgacaccgtc agtgcgtccg tcgcgcaggc tctcgatgag
     8701 ctgatgcttt gggccgagga ctgccccgaa gtccggcacc tcgtgcacgc ggatttcggc
     8761 tccaacaatg tcctgacgga caatggccgc ataacagcgg tcattgactg gagcgaggcg
     8821 atgttcgggg attcccaata cgaggtcgcc aacatcttct tctggaggcc gtggttggct
     8881 tgtatggagc agcagacgcg ctacttcgag cggaggcatc cggagcttgc aggatcgccg
     8941 cggctccggg cgtatatgct ccgcattggt cttgaccaac tctatcagag cttggttgac
     9001 ggcaatttcg atgatgcagc ttgggcgcag ggtcgatgcg acgcaatcgt ccgatccgga
     9061 gccgggactg tcgggcgtac acaaatcgcc cgcagaagcg cggccgtctg gaccgatggc
     9121 tgtgtagaag tactcgccga tagtggaaac cgacgcccca gcactcgtcc gagggcaaag
     9181 gaatagggta cctttaagac caatgactta caaggcagct gtagatctta gccacttttt
     9241 aaaagaaaag gggggactgg aagggctaat tcactcccaa cgaagacaag atctgctttt
     9301 tgcttgtact gggtctctct ggttagacca gatctgagcc tgggagctct ctggctaact
     9361 agggaaccca ctgcttaagc ctcaataaag cttgccttga gtgcttcaag tagtgtgtgc
     9421 ccgtctgttg tgtgactctg gtaactagag atccctcaga cccttttagt cagtgtggaa
     9481 aatctctagc agtagtagtt catgtcatct tattattcag tatttataac ttgcaaagaa
     9541 atgaatatca gagagtgaga ggaacttgtt tattgcagct tataatggtt acaaataaag
     9601 caatagcatc acaaatttca caaataaagc atttttttca ctgcattcta gttgtggttt
     9661 gtccaaactc atcaatgtat cttatcatgt ctggctctag ctatcccgcc cctaactccg
     9721 cccatcccgc ccctaactcc gcccagttcc gcccattctc cgccccatgg ctgactaatt
     9781 ttttttattt atgcagaggc cgaggccgcc tcggcctctg agctattcca gaagtagtga
     9841 ggaggctttt ttggaggcct agggacgtac ccaattcgcc ctatagtgag tcgtattacg
     9901 cgcgctcact ggccgtcgtt ttacaacgtc gtgactggga aaaccctggc gttacccaac
     9961 ttaatcgcct tgcagcacat ccccctttcg ccagctggcg taatagcgaa gaggcccgca
    10021 ccgatcgccc ttcccaacag ttgcgcagcc tgaatggcga atgggacgcg ccctgtagcg
    10081 gcgcattaag cgcggcgggt gtggtggtta cgcgcagcgt gaccgctaca cttgccagcg
    10141 ccctagcgcc cgctcctttc gctttcttcc cttcctttct cgccacgttc gccggctttc
    10201 cccgtcaagc tctaaatcgg gggctccctt tagggttccg atttagtgct ttacggcacc
    10261 tcgaccccaa aaaacttgat tagggtgatg gttcacgtag tgggccatcg ccctgataga
    10321 cggtttttcg ccctttgacg ttggagtcca cgttctttaa tagtggactc ttgttccaaa
    10381 ctggaacaac actcaaccct atctcggtct attcttttga tttataaggg attttgccga
    10441 tttcggccta ttggttaaaa aatgagctga tttaacaaaa atttaacgcg aattttaaca
    10501 aaatattaac gcttacaatt taggtggcac ttttcgggga aatgtgcgcg gaacccctat
    10561 ttgtttattt ttctaaatac attcaaatat gtatccgctc atgagacaat aaccctgata
    10621 aatgcttcaa taatattgaa aaaggaagag tatgagtatt caacatttcc gtgtcgccct
    10681 tattcccttt tttgcggcat tttgccttcc tgtttttgct cacccagaaa cgctggtgaa
    10741 agtaaaagat gctgaagatc agttgggtgc acgagtgggt tacatcgaac tggatctcaa
    10801 cagcggtaag atccttgaga gttttcgccc cgaagaacgt tttccaatga tgagcacttt
    10861 taaagttctg ctatgtggcg cggtattatc ccgtattgac gccgggcaag agcaactcgg
    10921 tcgccgcata cactattctc agaatgactt ggttgagtac tcaccagtca cagaaaagca
    10981 tcttacggat ggcatgacag taagagaatt atgcagtgct gccataacca tgagtgataa
    11041 cactgcggcc aacttacttc tgacaacgat cggaggaccg aaggagctaa ccgctttttt
    11101 gcacaacatg ggggatcatg taactcgcct tgatcgttgg gaaccggagc tgaatgaagc
    11161 cataccaaac gacgagcgtg acaccacgat gcctgtagca atggcaacaa cgttgcgcaa
    11221 actattaact ggcgaactac ttactctagc ttcccggcaa caattaatag actggatgga
    11281 ggcggataaa gttgcaggac cacttctgcg ctcggccctt ccggctggct ggtttattgc
    11341 tgataaatct ggagccggtg agcgtgggtc tcgcggtatc attgcagcac tggggccaga
    11401 tggtaagccc tcccgtatcg tagttatcta cacgacgggg agtcaggcaa ctatggatga
    11461 acgaaataga cagatcgctg agataggtgc ctcactgatt aagcattggt aactgtcaga
    11521 ccaagtttac tcatatatac tttagattga tttaaaactt catttttaat ttaaaaggat
    11581 ctaggtgaag atcctttttg ataatctcat gaccaaaatc ccttaacgtg agttttcgtt
    11641 ccactgagcg tcagaccccg tagaaaagat caaaggatct tcttgagatc ctttttttct
    11701 gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc
    11761 ggatcaagag ctaccaactc tttttccgaa ggtaactggc ttcagcagag cgcagatacc
    11821 aaatactgtt cttctagtgt agccgtagtt aggccaccac ttcaagaact ctgtagcacc
    11881 gcctacatac ctcgctctgc taatcctgtt accagtggct gctgccagtg gcgataagtc
    11941 gtgtcttacc gggttggact caagacgata gttaccggat aaggcgcagc ggtcgggctg
    12001 aacggggggt tcgtgcacac agcccagctt ggagcgaacg acctacaccg aactgagata
    12061 cctacagcgt gagctatgag aaagcgccac gcttcccgaa gagagaaagg cggacaggta
    12121 tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg gagcttccag ggggaaacgc
    12181 ctggtatctt tatagtcctg tcgggtttcg ccacctctga cttgagcgtc gatttttgtg
    12241 atgctcgtca ggggggcgga gcctatggaa aaacgccagc aacgcggcct ttttacggtt
    12301 cctggccttt tgctggcctt ttgctcacat gttctttcct gcgttatccc ctgattctgt
    12361 ggataaccgt attaccgcct ttgagtgagc tgataccgct cgccgcagcc gaacgaccga
    12421 gcgcagcgag tcagtgagcg aggaagcgga agagcgccca atacgcaaac cgcctctccc
    12481 cgcgcgttgg ccgattcatt aatgcagctg gcacgacagg tttcccgact ggaaagcggg
    12541 cagtgagcgc aacgcaatta atgtgagtta gctcactcat taggcacccc aggctttaca
    12601 ctttatgctt ccggctcgta tgttgtgtgg aattgtgagc ggataacaat ttcacacagg
    12661 aaacagctat gaccatgatt acgccaagcg cgcaattaac cctcactaaa gggaacaaaa
    12721 gctggagctg caagctt
//
