LOCUS       sequence-496331-       14519 bp    DNA     circular SYN 07-JAN-2026
DEFINITION  PARP1 and XRCC1 Split TurboID with a puromycin resistance cassette.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 14519)
  TITLE     replication stress 2
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 14519)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Jan 7, 2026 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: 
            pLV-Puro-EF1A-HA-PARP1-3xGS-TurboC-T2A-XRCC1-myc-3xGS-TurboN
FEATURES             Location/Qualifiers
     source          1..14519
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        3..229
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             230..410
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    457..582
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1075..1308
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1493..1537
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    1803..1920
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     protein_bind    1938..1958
                     /gene="mutant version of attB"
                     /label=attB4
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     promoter        1959..3137
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          2190..3128
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     3085..3105
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     protein_bind    3137..3161
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             3171..3197
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             6990..7043
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             8943..8972
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     protein_bind    complement(9192..9216)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     misc_feature    9234..9822
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(9287..9307)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(9705..9716)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     promoter        9853..10352
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(9888..9911)
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     primer_bind     10285..10304
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     CDS             10369..10968
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(10369..10388)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     10865..10885
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     LTR             11040..11273
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    11345..11466
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(11382..11401)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     11436..11455
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      11506..11641
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(11661..11680)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(11662..11680)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(11690..11707)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(11690..11706)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(11699..11721)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      11848..12303
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(11935..11954)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     12145..12166
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        12329..12433
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(12434..12502,12503..13294)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      13465..14053
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     13954..13973
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     14207..14224
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    14341..14362
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(14377..14382,14383..14400,14401..14407)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    14415..14431
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     14420..14442
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     14439..14455
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     14439..14455
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     14474..14494
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        14476..14494
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
ORIGIN
        1 aatgtagtct tatgcaatac tcttgtagtc ttgcaacatg gtaacgatga gttagcaaca
       61 tgccttacaa ggagagaaaa agcaccgtgc atgccgattg gtggaagtaa ggtggtacga
      121 tcgtgcctta ttaggaaggc aacagacggg tctgacatgg attggacgaa ccactgaatt
      181 gccgcattgc agagatattg tatttaagtg cctagctcga tacataaacg ggtctctctg
      241 gttagaccag atctgagcct gggagctctc tggctaacta gggaacccac tgcttaagcc
      301 tcaataaagc ttgccttgag tgcttcaagt agtgtgtgcc cgtctgttgt gtgactctgg
      361 taactagaga tccctcagac ccttttagtc agtgtggaaa atctctagca gtggcgcccg
      421 aacagggact tgaaagcgaa agggaaacca gaggagctct ctcgacgcag gactcggctt
      481 gctgaagcgc gcacggcaag aggcgagggg cggcgactgg tgagtacgcc aaaaattttg
      541 actagcggag gctagaagga gagagatggg tgcgagagcg tcagtattaa gcgggggaga
      601 attagatcgc gatgggaaaa aattcggtta aggccagggg gaaagaaaaa atataaatta
      661 aaacatatag tatgggcaag cagggagcta gaacgattcg cagttaatcc tggcctgtta
      721 gaaacatcag aaggctgtag acaaatactg ggacagctac aaccatccct tcagacagga
      781 tcagaagaac ttagatcatt atataataca gtagcaaccc tctattgtgt gcatcaaagg
      841 atagagataa aagacaccaa ggaagcttta gacaagatag aggaagagca aaacaaaagt
      901 aagaccaccg cacagcaagc ggccgctgat cttcagacct ggaggaggag atatgaggga
      961 caattggaga agtgaattat ataaatataa agtagtaaaa attgaaccat taggagtagc
     1021 acccaccaag gcaaagagaa gagtggtgca gagagaaaaa agagcagtgg gaataggagc
     1081 tttgttcctt gggttcttgg gagcagcagg aagcactatg ggcgcagcgt caatgacgct
     1141 gacggtacag gccagacaat tattgtctgg tatagtgcag cagcagaaca atttgctgag
     1201 ggctattgag gcgcaacagc atctgttgca actcacagtc tggggcatca agcagctcca
     1261 ggcaagaatc ctggctgtgg aaagatacct aaaggatcaa cagctcctgg ggatttgggg
     1321 ttgctctgga aaactcattt gcaccactgc tgtgccttgg aatgctagtt ggagtaataa
     1381 atctctggaa cagatttgga atcacacgac ctggatggag tgggacagag aaattaacaa
     1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga
     1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa
     1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat
     1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt
     1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg
     1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggtatcgcta
     1801 gcttttaaaa gaaaaggggg gattgggggg tacagtgcag gggaaagaat agtagacata
     1861 atagcaacag acatacaaac taaagaatta caaaaacaaa ttacaaaaat tcaaaatttt
     1921 actagtgatt atcggatcaa ctttgtatag aaaagttggg ctccggtgcc cgtcagtggg
     1981 cagagcgcac atcgcccaca gtccccgaga agttgggggg aggggtcggc aattgaaccg
     2041 gtgcctagag aaggtggcgc ggggtaaact gggaaagtga tgtcgtgtac tggctccgcc
     2101 tttttcccga gggtggggga gaaccgtata taagtgcagt agtcgccgtg aacgttcttt
     2161 ttcgcaacgg gtttgccgcc agaacacagg taagtgccgt gtgtggttcc cgcgggcctg
     2221 gcctctttac gggttatggc ccttgcgtgc cttgaattac ttccacctgg ctgcagtacg
     2281 tgattcttga tcccgagctt cgggttggaa gtgggtggga gagttcgagg ccttgcgctt
     2341 aaggagcccc ttcgcctcgt gcttgagttg aggcctggcc tgggcgctgg ggccgccgcg
     2401 tgcgaatctg gtggcacctt cgcgcctgtc tcgctgcttt cgataagtct ctagccattt
     2461 aaaatttttg atgacctgct gcgacgcttt ttttctggca agatagtctt gtaaatgcgg
     2521 gccaagatct gcacactggt atttcggttt ttggggccgc gggcggcgac ggggcccgtg
     2581 cgtcccagcg cacatgttcg gcgaggcggg gcctgcgagc gcggccaccg agaatcggac
     2641 gggggtagtc tcaagctggc cggcctgctc tggtgcctgg tctcgcgccg ccgtgtatcg
     2701 ccccgccctg ggcggcaagg ctggcccggt cggcaccagt tgcgtgagcg gaaagatggc
     2761 cgcttcccgg ccctgctgca gggagctcaa aatggaggac gcggcgctcg ggagagcggg
     2821 cgggtgagtc acccacacaa aggaaaaggg cctttccgtc ctcagccgtc gcttcatgtg
     2881 actccacgga gtaccgggcg ccgtccaggc acctcgatta gttctcgagc ttttggagta
     2941 cgtcgtcttt aggttggggg gaggggtttt atgcgatgga gtttccccac actgagtggg
     3001 tggagactga agttaggcca gcttggcact tgatgtaatt ctccttggaa tttgcccttt
     3061 ttgagtttgg atcttggttc attctcaagc ctcagacagt ggttcaaagt ttttttcttc
     3121 catttcaggt gtcgtgacaa gtttgtacaa aaaagcaggc tgccaccatg tacccatacg
     3181 atgttccaga ttacgctgcg gagtcttcgg ataagctcta tcgagtcgag tacgccaaga
     3241 gcgggcgcgc ctcttgcaag aaatgcagcg agagcatccc caaggactcg ctccggatgg
     3301 ccatcatggt gcagtcgccc atgtttgatg gaaaagtccc acactggtac cacttctcct
     3361 gcttctggaa ggtgggccac tccatccggc accctgacgt tgaggtggat gggttctctg
     3421 agcttcggtg ggatgaccag cagaaagtca agaagacagc ggaagctgga ggagtgacag
     3481 gcaaaggcca ggatggaatt ggtagcaagg cagagaagac tctgggtgac tttgcagcag
     3541 agtatgccaa gtccaacaga agtacgtgca aggggtgtat ggagaagata gaaaagggcc
     3601 aggtgcgcct gtccaagaag atggtggacc cggagaagcc acagctaggc atgattgacc
     3661 gctggtacca tccaggctgc tttgtcaaga acagggagga gctgggtttc cggcccgagt
     3721 acagtgcgag tcagctcaag ggcttcagcc tccttgctac agaggataaa gaagccctga
     3781 agaagcagct cccaggagtc aagagtgaag gaaagagaaa aggcgatgag gtggatggag
     3841 tggatgaagt ggcgaagaag aaatctaaaa aagaaaaaga caaggatagt aagcttgaaa
     3901 aagccctaaa ggctcagaac gacctgatct ggaacatcaa ggacgagcta aagaaagtgt
     3961 gttcaactaa tgacctgaag gagctactca tcttcaacaa gcagcaagtg ccttctgggg
     4021 agtcggcgat cttggaccga gtagctgatg gcatggtgtt cggtgccctc cttccctgcg
     4081 aggaatgctc gggtcagctg gtcttcaaga gcgatgccta ttactgcact ggggacgtca
     4141 ctgcctggac caagtgtatg gtcaagacac agacacccaa ccggaaggag tgggtaaccc
     4201 caaaggaatt ccgagaaatc tcttacctca agaaattgaa ggttaaaaaa caggaccgta
     4261 tattcccccc agaaaccagc gcctccgtgg cggccacgcc tccgccctcc acagcctcgg
     4321 ctcctgctgc tgtgaactcc tctgcttcag cagataagcc attatccaac atgaagatcc
     4381 tgactctcgg gaagctgtcc cggaacaagg atgaagtgaa ggccatgatt gagaaactcg
     4441 gggggaagtt gacggggacg gccaacaagg cttccctgtg catcagcacc aaaaaggagg
     4501 tggaaaagat gaataagaag atggaggaag taaaggaagc caacatccga gttgtgtctg
     4561 aggacttcct ccaggacgtc tccgcctcca ccaagagcct tcaggagttg ttcttagcgc
     4621 acatcttgtc cccttggggg gcagaggtga aggcagagcc tgttgaagtt gtggccccaa
     4681 gagggaagtc aggggctgcg ctctccaaaa aaagcaaggg ccaggtcaag gaggaaggta
     4741 tcaacaaatc tgaaaagaga atgaaattaa ctcttaaagg aggagcagct gtggatcctg
     4801 attctggact ggaacactct gcgcatgtcc tggagaaagg tgggaaggtc ttcagtgcca
     4861 cccttggcct ggtggacatc gttaaaggaa ccaactccta ctacaagctg cagcttctgg
     4921 aggacgacaa ggaaaacagg tattggatat tcaggtcctg gggccgtgtg ggtacggtga
     4981 tcggtagcaa caaactggaa cagatgccgt ccaaggagga tgccattgag cacttcatga
     5041 aattatatga agaaaaaacc gggaacgctt ggcactccaa aaatttcacg aagtatccca
     5101 aaaagttcta ccccctggag attgactatg gccaggatga agaggcagtg aagaagctga
     5161 cagtaaatcc tggcaccaag tccaagctcc ccaagccagt tcaggacctc atcaagatga
     5221 tctttgatgt ggaaagtatg aagaaagcca tggtggagta tgagatcgac cttcagaaga
     5281 tgcccttggg gaagctgagc aaaaggcaga tccaggccgc atactccatc ctcagtgagg
     5341 tccagcaggc ggtgtctcag ggcagcagcg actctcagat cctggatctc tcaaatcgct
     5401 tttacaccct gatcccccac gactttggga tgaagaagcc tccgctcctg aacaatgcag
     5461 acagtgtgca ggccaaggtg gaaatgcttg acaacctgct ggacatcgag gtggcctaca
     5521 gtctgctcag gggagggtct gatgatagca gcaaggatcc catcgatgtc aactatgaga
     5581 agctcaaaac tgacattaag gtggttgaca gagattctga agaagccgag atcatcagga
     5641 agtatgttaa gaacactcat gcaaccacac acaatgcgta tgacttggaa gtcatcgata
     5701 tctttaagat agagcgtgaa ggcgaatgcc agcgttacaa gccctttaag cagcttcata
     5761 accgaagatt gctgtggcac gggtccagga ccaccaactt tgctgggatc ctgtcccagg
     5821 gtcttcggat agccccgcct gaagcgcccg tgacaggcta catgtttggt aaagggatct
     5881 atttcgctga catggtctcc aagagtgcca actactgcca tacgtctcag ggagacccaa
     5941 taggcttaat cctgttggga gaagttgccc ttggaaacat gtatgaactg aagcacgctt
     6001 cacatatcag caagttaccc aagggcaagc acagtgtcaa aggtttgggc aaaactaccc
     6061 ctgatccttc agctaacatt agtctggatg gtgtagacgt tcctcttggg accgggattt
     6121 catctggtgt gaatgacacc tctctactat ataacgagta cattgtctat gatattgctc
     6181 aggtaaatct gaagtatctg ctgaaactga aattcaattt taagacctcc ctgtggggac
     6241 agctggacgg cgggagcgtg gcagtcctgc ctgtggtcga ctccaccaat cagtacctgc
     6301 tggatcgaat cggcgagctg aagagtgggg atgcttgcat tgcagaatat cagcaggcag
     6361 ggagaggaag cagagggagg aaatggttct ctccttttgg agctaacctg tacctgagta
     6421 tgttttggcg cctgaagcgg ggaccagcag caatcggcct gggcccggtc atcggaattg
     6481 tcatggcaga agcgctgcga aagctgggag cagacaaggt gcgagtcaaa tggcccaatg
     6541 acctgtatct gcaggataga aagctggcag gcatcctggt ggagctggcc ggaataacag
     6601 gcgatgctgc acagatcgtc attggcgccg ggattaacgt ggctatgagg cgcgtggagg
     6661 aaagcgtggt caatcagggc tggatcacac tgcaggaagc agggattaac ctggacagga
     6721 atactctggc cgctacgctg atccgagagc tgcgggcagc cctggaactg ttcgagcagg
     6781 aaggcctggc tccatatctg ccacggtggg agaagctgga taacttcatc aatagacccg
     6841 tgaagctgat cattggggac aaagagattt tcgggattag ccgggggatt gataaacagg
     6901 gagccctgct gctggaacag gacggagtta tcaaaccctg gatgggcgga gaaatcagtc
     6961 tgcggtctgc cgaaaaggct ggaagcggag agggcagggg aagtcttcta acatgcgggg
     7021 acgtggagga aaatcccggc cccatgccgg agatccgcct ccgccatgtc gtgtcctgca
     7081 gcagccagga ctcgactcac tgtgcagaaa atcttctcaa ggcagacact taccgaaaat
     7141 ggcgggcagc caaggcaggc gagaagacca tctctgtggt cctacagttg gagaaggagg
     7201 agcagataca cagtgtggac attgggaatg atggctcagc tttcgtggag gtgctggtgg
     7261 gcagttcagc tggaggcgct ggggagcaag actatgaggt ccttctggtc acctcatctt
     7321 tcatgtcccc ttccgagagc cgcagtggct caaaccccaa ccgcgttcgc atgtttgggc
     7381 ctgacaagct ggtccgggca gccgccgaga agcgctggga ccgggtcaaa attgtttgca
     7441 gccagcccta cagcaaggac tccccctttg gcttgagttt tgtacggttt catagccccc
     7501 cagacaaaga tgaggcagag gccccgtccc agaaggtgac agtgaccaag cttggccagt
     7561 tccgtgtgaa ggaggaggat gagagcgcca actctctgag gccgggggct ctcttcttca
     7621 gccggatcaa caagacatcc ccagtcacag ccagcgaccc agcaggacct agctatgcag
     7681 ctgctaccct ccaggcttct agtgctgcct cctcagcctc tccagtctcc agggccatag
     7741 gcagcacctc caagccccag gagtctccca aagggaagag gaagttggat ttgaaccaag
     7801 aagaaaagaa gacccccagc aaaccaccag cccagctgtc gccatctgtt cccaagagac
     7861 ctaaattgcc agctccaact cgtaccccag ccacagcccc agtccctgcc cgagcacagg
     7921 gggcagtgac aggcaaaccc cgaggagaag gcaccgagcc cagacgaccc cgagctggcc
     7981 cagaggagct ggggaagatc cttcagggtg tggtagtggt gctgagtggc ttccagaacc
     8041 ccttccgctc cgagctgcga gataaggccc tagagcttgg ggccaagtat cggccagact
     8101 ggacccggga cagcacgcac ctcatctgtg cctttgccaa cacccccaag tacagccagg
     8161 tcctaggcct gggaggccgc atcgtgcgta aggagtgggt gctggactgt caccgcatgc
     8221 gtcggcggct gccctcccag aggtacctca tggcagggcc aggttccagc agtgaggagg
     8281 atgaggcctc tcacagcggt ggcagcggag atgaagcccc caagcttcct cagaagcaac
     8341 cccagaccaa aaccaagccc actcaggcag ctggacccag ctcaccccag aagcccccaa
     8401 cccctgaaga gaccaaagca gcctcaccag tgctccagga agatatagac attgaggggg
     8461 tacagtcaga aggacaggac aatggggcgg aagattctgg ggacacagag gatgagctga
     8521 ggagggtggc agagcagaag gaacacagac tgccccctgg ccaggaggag aatggggaag
     8581 acccgtatgc aggctccacg gatgagaaca cggacagtga ggaacaccag gagcctcctg
     8641 atctgccagt ccctgagctc ccagatttct tccagggcaa gcacttcttt ctttacgggg
     8701 agttccctgg ggacgagcgg cggaaactca tccgatacgt cacagccttc aatggggagc
     8761 tcgaggacta tatgagtgac cgggttcagt ttgtgatcac agcacaggaa tgggatccca
     8821 gctttgagga ggccctgatg gacaacccct ccctggcatt cgttcgtccc cgatggatct
     8881 acagttgcaa tgagaagcag aagttacttc ctcaccagct ctatggggtg gtgccgcaag
     8941 ccgaacaaaa actcatctca gaagaggatc tgaaagacaa tactgtgcct ctgaagctga
     9001 tcgctctcct ggctaatggc gagttccata gtggcgaaca gctgggagaa accctgggca
     9061 tgtccagggc cgctatcaac aagcacattc agactctgcg cgactggggc gtggacgtgt
     9121 tcaccgtgcc cggaaagggc tactctctgc ccgagcctat cccgctgctg aacgctaaac
     9181 agattctgtg aacccagctt tcttgtacaa agtggtgata atcgaattcc gataatcaac
     9241 ctctggatta caaaatttgt gaaagattga ctggtattct taactatgtt gctcctttta
     9301 cgctatgtgg atacgctgct ttaatgcctt tgtatcatgc tattgcttcc cgtatggctt
     9361 tcattttctc ctccttgtat aaatcctggt tgctgtctct ttatgaggag ttgtggcccg
     9421 ttgtcaggca acgtggcgtg gtgtgcactg tgtttgctga cgcaaccccc actggttggg
     9481 gcattgccac cacctgtcag ctcctttccg ggactttcgc tttccccctc cctattgcca
     9541 cggcggaact catcgccgcc tgccttgccc gctgctggac aggggctcgg ctgttgggca
     9601 ctgacaattc cgtggtgttg tcggggaagc tgacgtcctt tccatggctg ctcgcctgtg
     9661 ttgccacctg gattctgcgc gggacgtcct tctgctacgt cccttcggcc ctcaatccag
     9721 cggaccttcc ttcccgcggc ctgctgccgg ctctgcggcc tcttccgcgt cttcgccttc
     9781 gccctcagac gagtcggatc tccctttggg ccgcctcccc gcatcgggaa ttcccgcggt
     9841 tcgaattcta ccgggtaggg gaggcgcttt tcccaaggca gtctggagca tgcgctttag
     9901 cagccccgct gggcacttgg cgctacacaa gtggcctctg gcctcgcaca cattccacat
     9961 ccaccggtag gcgccaaccg gctccgttct ttggtggccc cttcgcgcca ccttctactc
    10021 ctcccctagt caggaagttc ccccccgccc cgcagctcgc gtcgtgcagg acgtgacaaa
    10081 tggaagtagc acgtctcact agtctcgtgc agatggacag caccgctgag caatggaagc
    10141 gggtaggcct ttggggcagc ggccaatagc agctttgctc cttcgctttc tgggctcaga
    10201 ggctgggaag gggtgggtcc gggggcgggc tcaggggcgg gctcaggggc ggggcgggcg
    10261 cccgaaggtc ctccggaggc ccggcattct gcacgcttca aaagcgcacg tctgccgcgc
    10321 tgttctcctc ttcctcatct ccgggccttt cgacctcacg tggccaccat gaccgagtac
    10381 aagcccacgg tgcgcctcgc cacccgcgac gacgtcccca gggccgtacg caccctcgcc
    10441 gccgcgttcg ccgactaccc cgccacgcgc cacaccgtcg atccggaccg ccacatcgag
    10501 cgggtcaccg agctgcaaga actcttcctc acgcgcgtcg ggctcgacat cggcaaggtg
    10561 tgggtcgcgg acgacggcgc cgcggtggcg gtctggacca cgccggagag cgtcgaagcg
    10621 ggggcggtgt tcgccgagat cggcccgcgc atggccgagt tgagcggttc ccggctggcc
    10681 gcgcagcaac agatggaagg cctcctggcg ccgcaccggc ccaaggagcc cgcgtggttc
    10741 ctggccaccg tcggcgtctc gcccgaccac cagggcaagg gtctgggcag cgccgtcgtg
    10801 ctccccggag tggaggcggc cgagcgcgcc ggggtgcccg ccttcctgga gacctccgcg
    10861 ccccgcaacc tccccttcta cgagcggctc ggcttcaccg tcaccgccga cgtcgaggtg
    10921 cccgaaggac cgcgcacctg gtgcatgacc cgcaagcccg gtgcctgagg tacctttaag
    10981 accaatgact tacaaggcag ctgtagatct tagccacttt ttaaaagaaa aggggggact
    11041 ggaagggcta attcactccc aacgaagaca agatctgctt tttgcttgta ctgggtctct
    11101 ctggttagac cagatctgag cctgggagct ctctggctaa ctagggaacc cactgcttaa
    11161 gcctcaataa agcttgcctt gagtgcttca agtagtgtgt gcccgtctgt tgtgtgactc
    11221 tggtaactag agatccctca gaccctttta gtcagtgtgg aaaatctcta gcagtagtag
    11281 ttcatgtcat cttattattc agtatttata acttgcaaag aaatgaatat cagagagtga
    11341 gaggaacttg tttattgcag cttataatgg ttacaaataa agcaatagca tcacaaattt
    11401 cacaaataaa gcattttttt cactgcattc tagttgtggt ttgtccaaac tcatcaatgt
    11461 atcttatcat gtctggctct agctatcccg cccctaactc cgcccatccc gcccctaact
    11521 ccgcccagtt ccgcccattc tccgccccat ggctgactaa ttttttttat ttatgcagag
    11581 gccgaggccg cctcggcctc tgagctattc cagaagtagt gaggaggctt ttttggaggc
    11641 ctagggacgt acccaattcg ccctatagtg agtcgtatta cgcgcgctca ctggccgtcg
    11701 ttttacaacg tcgtgactgg gaaaaccctg gcgttaccca acttaatcgc cttgcagcac
    11761 atcccccttt cgccagctgg cgtaatagcg aagaggcccg caccgatcgc ccttcccaac
    11821 agttgcgcag cctgaatggc gaatgggacg cgccctgtag cggcgcatta agcgcggcgg
    11881 gtgtggtggt tacgcgcagc gtgaccgcta cacttgccag cgccctagcg cccgctcctt
    11941 tcgctttctt cccttccttt ctcgccacgt tcgccggctt tccccgtcaa gctctaaatc
    12001 gggggctccc tttagggttc cgatttagtg ctttacggca cctcgacccc aaaaaacttg
    12061 attagggtga tggttcacgt agtgggccat cgccctgata gacggttttt cgccctttga
    12121 cgttggagtc cacgttcttt aatagtggac tcttgttcca aactggaaca acactcaacc
    12181 ctatctcggt ctattctttt gatttataag ggattttgcc gatttcggcc tattggttaa
    12241 aaaatgagct gatttaacaa aaatttaacg cgaattttaa caaaatatta acgcttacaa
    12301 tttaggtggc acttttcggg gaaatgtgcg cggaacccct atttgtttat ttttctaaat
    12361 acattcaaat atgtatccgc tcatgagaca ataaccctga taaatgcttc aataatattg
    12421 aaaaaggaag agtatgagta ttcaacattt ccgtgtcgcc cttattccct tttttgcggc
    12481 attttgcctt cctgtttttg ctcacccaga aacgctggtg aaagtaaaag atgctgaaga
    12541 tcagttgggt gcacgagtgg gttacatcga actggatctc aacagcggta agatccttga
    12601 gagttttcgc cccgaagaac gttttccaat gatgagcact tttaaagttc tgctatgtgg
    12661 cgcggtatta tcccgtattg acgccgggca agagcaactc ggtcgccgca tacactattc
    12721 tcagaatgac ttggttgagt actcaccagt cacagaaaag catcttacgg atggcatgac
    12781 agtaagagaa ttatgcagtg ctgccataac catgagtgat aacactgcgg ccaacttact
    12841 tctgacaacg atcggaggac cgaaggagct aaccgctttt ttgcacaaca tgggggatca
    12901 tgtaactcgc cttgatcgtt gggaaccgga gctgaatgaa gccataccaa acgacgagcg
    12961 tgacaccacg atgcctgtag caatggcaac aacgttgcgc aaactattaa ctggcgaact
    13021 acttactcta gcttcccggc aacaattaat agactggatg gaggcggata aagttgcagg
    13081 accacttctg cgctcggccc ttccggctgg ctggtttatt gctgataaat ctggagccgg
    13141 tgagcgtggg tctcgcggta tcattgcagc actggggcca gatggtaagc cctcccgtat
    13201 cgtagttatc tacacgacgg ggagtcaggc aactatggat gaacgaaata gacagatcgc
    13261 tgagataggt gcctcactga ttaagcattg gtaactgtca gaccaagttt actcatatat
    13321 actttagatt gatttaaaac ttcattttta atttaaaagg atctaggtga agatcctttt
    13381 tgataatctc atgaccaaaa tcccttaacg tgagttttcg ttccactgag cgtcagaccc
    13441 cgtagaaaag atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt
    13501 gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg ccggatcaag agctaccaac
    13561 tctttttccg aaggtaactg gcttcagcag agcgcagata ccaaatactg ttcttctagt
    13621 gtagccgtag ttaggccacc acttcaagaa ctctgtagca ccgcctacat acctcgctct
    13681 gctaatcctg ttaccagtgg ctgctgccag tggcgataag tcgtgtctta ccgggttgga
    13741 ctcaagacga tagttaccgg ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac
    13801 acagcccagc ttggagcgaa cgacctacac cgaactgaga tacctacagc gtgagctatg
    13861 agaaagcgcc acgcttcccg aagagagaaa ggcggacagg tatccggtaa gcggcagggt
    13921 cggaacagga gagcgcacga gggagcttcc agggggaaac gcctggtatc tttatagtcc
    13981 tgtcgggttt cgccacctct gacttgagcg tcgatttttg tgatgctcgt caggggggcg
    14041 gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct tttgctggcc
    14101 ttttgctcac atgttctttc ctgcgttatc ccctgattct gtggataacc gtattaccgc
    14161 ctttgagtga gctgataccg ctcgccgcag ccgaacgacc gagcgcagcg agtcagtgag
    14221 cgaggaagcg gaagagcgcc caatacgcaa accgcctctc cccgcgcgtt ggccgattca
    14281 ttaatgcagc tggcacgaca ggtttcccga ctggaaagcg ggcagtgagc gcaacgcaat
    14341 taatgtgagt tagctcactc attaggcacc ccaggcttta cactttatgc ttccggctcg
    14401 tatgttgtgt ggaattgtga gcggataaca atttcacaca ggaaacagct atgaccatga
    14461 ttacgccaag cgcgcaatta accctcacta aagggaacaa aagctggagc tgcaagctt
//
