LOCUS       sequence-496341-        9700 bp    DNA     circular SYN 07-JAN-2026
DEFINITION  Empty vector for generating yeast 2-hybrid GAL4 activation domain 
            fusion constructs by Gateway cloning. It may also be used as an 
            empty vector experimental control..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9700)
  AUTHORS   Rossignol P, Collier S, Bush M, Shaw P, Doonan JH
  TITLE     Arabidopsis POT1A interacts with TERT-V(I8), an N-terminal splicing 
            variant of telomerase.
  JOURNAL   J Cell Sci. 2007 Oct 15;120(Pt 20):3678-87. doi: 10.1242/jcs.004119.
            Epub 2007 Oct 2.
  PUBMED    17911168
REFERENCE   2  (bases 1 to 9700)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Jan 7, 2026 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Cell 
            Sci."; date: "2007-10-15"; volume: "120(Pt 20):3678-87. doi"; pages:
            " 10.1242/jcs.004119. Epub 2007 Oct 2"
COMMENT     Sequence Label: pDEST-GADT7
FEATURES             Location/Qualifiers
     source          1..9700
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        771..1475
                     /gene="S. cerevisiae ADH1"
                     /label=ADH1 promoter
                     /note="promoter for alcohol dehydrogenase 1"
     CDS             1521..1541
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             1557..1898
                     /codon_start=1
                     /gene="Saccharomyces cerevisiae GAL4 (truncated)"
                     /product="activation domain of the GAL4 transcriptional 
                     activator"
                     /label=GAL4 activation domain
                     /translation="ANFNQSGNIADSSLSFTFTNSSNGPNLITTQTNSQALSQPIASSN
                     VHDNFMNNEITASKIDDGNNSKPLSPGWTDQTAYNAFGITTGMFNTTTMDDVYNYLFDD
                     EDTPPNPKKE"
     primer_bind     1904..1923
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        1904..1922
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     regulatory      1929..1938
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             1941..1967
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     protein_bind    2004..2128
                     /gene="mutant version of attR"
                     /label=attR1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     promoter        join(2153..2158,2159..2176,2177..2183)
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an ""up"" mutation"
     CDS             2237..2896
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     complement(2303..2322)
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     CDS             3238..3543
                     /codon_start=1
                     /gene="ccdB"
                     /product="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /label=ccdB
                     /note="Plasmids containing the ccdB gene cannot be 
                     propagated in standard E. coli strains."
                     /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK
                     VSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     primer_bind     3422..3441
                     /label=ccdB-fwd
                     /note="ccdB gene, forward primer"
     protein_bind    complement(3584..3708)
                     /gene="mutant version of attR"
                     /label=attR2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     terminator      4128..4315
                     /gene="S. cerevisiae ADH1"
                     /label=ADH1 terminator
                     /note="transcription terminator for the S. cerevisiae 
                     alcohol dehydrogenase 1 (ADH1) gene"
     primer_bind     complement(4183..4207)
                     /label=pHybLex-R
                     /note="ADH terminator, reverse primer"
     CDS             complement(4432..5526)
                     /codon_start=1
                     /gene="S. cerevisiae LEU2"
                     /product="3-isopropylmalate dehydrogenase, required for 
                     leucine biosynthesis"
                     /label=LEU2
                     /note="yeast auxotrophic marker"
                     /translation="MSAPKKIVVLPGDHVGQEITAEAIKVLKAISDVRSNVKFDFENHL
                     IGGAAIEATGVPLPDEALEASKKADAVLLGAVGGPKWGTGSVRPEQGLLKIRKELQLYA
                     NLRPCNFASDSLLDLSPIKPQFAKGTDFVVVRELVGGIYFGKRKEDDGDGVAWDSEQYT
                     VPEVQRITRMAAFMALQHEPPLPIWSLDKANVLASSRLWRKTVEETIKNEFPTLKVQHQ
                     LIDSAAMILVKNPTHLNGIIITSNMFGDIISDEASVIPGSLGLLPSASLASLPDKNTAF
                     GLYEPCHGSAPDLPKNKVNPIATILSAAMMLKLSLNLPEEGKAIEDAVKKVLDAGIRTG
                     DLGGSNSTTEVGDAVAEEVKKILA"
     promoter        complement(5527..5932)
                     /gene="S. cerevisiae LEU2"
                     /label=LEU2 promoter
     primer_bind     complement(5974..5990)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5974..5990)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(5987..6009)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    5998..6014
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(6022..6028,6029..6046,6047..6052))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6067..6088
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     protein_bind    6143..6176
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     protein_bind    6254..6287
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     primer_bind     complement(6356..6373)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(6527..7115)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(6607..6626)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(7286..8077,8078..8146))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     7909..7928
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(8147..8251)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     8319..8337
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(8375..8397)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     8497..8516
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     rep_origin      8533..9697
                     /label=2u ori
                     /note="yeast 2u plasmid origin of replication"
ORIGIN
        1 tgcatgcctg caggtcgaga tccgggatcg aagaaatgat ggtaaatgaa ataggaaatc
       61 aaggagcatg aaggcaaaag acaaatataa gggtcgaacg aaaaataaag tgaaaagtgt
      121 tgatatgatg tatttggctt tgcggcgccg aaaaaacgag tttacgcaat tgcacaatca
      181 tgctgactct gtggcggacc cgcgctcttg ccggcccggc gataacgctg ggcgtgaggc
      241 tgtgcccggc ggagtttttt gcgcctgcat tttccaaggt ttaccctgcg ctaaggggcg
      301 agattggaga agcaataaga atgccggttg gggttgcgat gatgacgacc acgacaactg
      361 gtgtcattat ttaagttgcc gaaagaacct gagtgcattt gcaacatgag tatactagaa
      421 gaatgagcca agacttgcga gacgcgagtt tgccggtggt gcgaacaata gagcgaccat
      481 gaccttgaag gtgagacgcg cataaccgct agagtacttt gaagaggaaa cagcaatagg
      541 gttgctacca gtataaatag acaggtacat acaacactgg aaatggttgt ctgtttgagt
      601 acgctttcaa ttcatttggg tgtgcacttt attatgttac aatatggaag ggaactttac
      661 acttctccta tgcacatata ttaattaaag tccaatgcta gtagagaagg ggggtaacac
      721 ccctccgcgc tcttttccga tttttttcta aaccgtggaa tatttcggat atccttttgt
      781 tgtttccggg tgtacaatat ggacttcctc ttttctggca accaaaccca tacatcggga
      841 ttcctataat accttcgttg gtctccctaa catgtaggtg gcggagggga gatatacaat
      901 agaacagata ccagacaaga cataatgggc taaacaagac tacaccaatt acactgcctc
      961 attgatggtg gtacataacg aactaatact gtagccctag acttgatagc catcatcata
     1021 tcgaagtttc actacccttt ttccatttgc catctattga agtaataata ggcgcatgca
     1081 acttcttttc tttttttttc ttttctctct cccccgttgt tgtctcacca tatccgcaat
     1141 gacaaaaaaa tgatggaaga cactaaagga aaaaattaac gacaaagaca gcaccaacag
     1201 atgtcgttgt tccagagctg atgaggggta tctcgaagca cacgaaactt tttccttcct
     1261 tcattcacgc acactactct ctaatgagca acggtatacg gccttccttc cagttacttg
     1321 aatttgaaat aaaaaaaagt ttgctgtctt gctatcaagt ataaatagac ctgcaattat
     1381 taatcttttg tttcctcgtc attgttctcg ttccctttct tccttgtttc tttttctgca
     1441 caatatttca agctatacca agcatacaat caactccaag ctttgcaaag atggataaag
     1501 cggaattaat tcccgagcct ccaaaaaaga agagaaaggt cgaattgggt accgccgcca
     1561 attttaatca aagtgggaat attgctgata gctcattgtc cttcactttc actaacagta
     1621 gcaacggtcc gaacctcata acaactcaaa caaattctca agcgctttca caaccaattg
     1681 cctcctctaa cgttcatgat aacttcatga ataatgaaat cacggctagt aaaattgatg
     1741 atggtaataa ttcaaaacca ctgtcacctg gttggacgga ccaaactgcg tataacgcgt
     1801 ttggaatcac tacagggatg tttaatacca ctacaatgga tgatgtatat aactatctat
     1861 tcgatgatga agatacccca ccaaacccaa aaaaagagat ctttaatacg actcactata
     1921 gggcgagcgc cgccatggag tacccatacg acgtaccaga ttacgctcat atggccatgg
     1981 aggccagtga attccaccca tcaacaagtt tgtacaaaaa agctgaacga gaaacgtaaa
     2041 atgatataaa tatcaatata ttaaattaga ttttgcataa aaaacagact acataatact
     2101 gtaaaacaca acatatccag tcactatggc ggccgcatta ggcaccccag gctttacact
     2161 ttatgcttcc ggctcgtata atgtgtggat tttgagttag gatccgtcga gattttcagg
     2221 agctaaggaa gctaaaatgg agaaaaaaat cactggatat accaccgttg atatatccca
     2281 atggcatcgt aaagaacatt ttgaggcatt tcagtcagtt gctcaatgta cctataacca
     2341 gaccgttcag ctggatatta cggccttttt aaagaccgta aagaaaaata agcacaagtt
     2401 ttatccggcc tttattcaca ttcttgcccg cctgatgaat gctcatccgg aattccgtat
     2461 ggcaatgaaa gacggtgagc tggtgatatg ggatagtgtt cacccttgtt acaccgtttt
     2521 ccatgagcaa actgaaacgt tttcatcgct ctggagtgaa taccacgacg atttccggca
     2581 gtttctacac atatattcgc aagatgtggc gtgttacggt gaaaacctgg cctatttccc
     2641 taaagggttt attgagaata tgtttttcgt ctcagccaat ccctgggtga gtttcaccag
     2701 ttttgattta aacgtggcca atatggacaa cttcttcgcc cccgttttca ccatgggcaa
     2761 atattatacg caaggcgaca aggtgctgat gccgctggcg attcaggttc atcatgccgt
     2821 ttgtgatggc ttccatgtcg gcagaatgct taatgaatta caacagtact gcgatgagtg
     2881 gcagggcggg gcgtaaagat ctggatccgg cttactaaaa gccagataac agtatgcgta
     2941 tttgcgcgct gatttttgcg gtataagaat atatactgat atgtataccc gaagtatgtc
     3001 aaaaagaggt atgctatgaa gcagcgtatt acagtgacag ttgacagcga cagctatcag
     3061 ttgctcaagg catatatgat gtcaatatct ccggtctggt aagcacaacc atgcagaatg
     3121 aagcccgtcg tctgcgtgcc gaacgctgga aagcggaaaa tcaggaaggg atggctgagg
     3181 tcgcccggtt tattgaaatg aacggctctt ttgctgacga gaacaggggc tggtgaaatg
     3241 cagtttaagg tttacaccta taaaagagag agccgttatc gtctgtttgt ggatgtacag
     3301 agtgatatta ttgacacgcc cgggcgacgg atggtgatcc ccctggccag tgcacgtctg
     3361 ctgtcagata aagtctcccg tgaactttac ccggtggtgc atatcgggga tgaaagctgg
     3421 cgcatgatga ccaccgatat ggccagtgtg ccggtctccg ttatcgggga agaagtggct
     3481 gatctcagcc accgcgaaaa tgacatcaaa aacgccatta acctgatgtt ctggggaata
     3541 taaatgtcag gctcccttat acacagccag tctgcaggtc gaccatagtg actggatatg
     3601 ttgtgtttta cagtattatg tagtctgttt tttatgcaaa atctaattta atatattgat
     3661 atttatatca ttttacgttt ctcgttcagc tttcttgtac aaagtggttg atgggtgggc
     3721 atcgatacgg gatccatcga gctcgagctg cagatgaatc gtagatactg aaaaaccccg
     3781 caagttcact tcaactgtgc atcgtgcacc atctcaattt ctttcattta tacatcgttt
     3841 tgccttcttt tatgtaacta tactcctcta agtttcaatc ttggccatgt aacctctgat
     3901 ctatagaatt ttttaaatga ctagaattaa tgcccatctt ttttttggac ctaaattctt
     3961 catgaaaata tattacgagg gcttattcag aagctttgga cttcttcgcc agaggtttgg
     4021 tcaagtctcc aatcaaggtt gtcggcttgt ctaccttgcc agaaatttac gaaaagatgg
     4081 aaaagggtca aatcgttggt agatacgttg ttgacacttc taaataagcg aatttcttat
     4141 gatttatgat ttttattatt aaataagtta taaaaaaaat aagtgtatac aaattttaaa
     4201 gtgactctta ggttttaaaa cgaaaattct tattcttgag taactctttc ctgtaggtca
     4261 ggttgctttc tcaggtatag catgaggtcg ctcttattga ccacacctct accggccggt
     4321 cgaaattccc ctaccctatg aacatattcc attttgtaat ttcgtgtcgt ttctattatg
     4381 aatttcattt ataaagttta tgtacaaata tcataaaaaa agagaatctt tttaagcaag
     4441 gattttctta acttcttcgg cgacagcatc accgacttcg gtggtactgt tggaaccacc
     4501 taaatcacca gttctgatac ctgcatccaa aaccttttta actgcatctt caatggcctt
     4561 accttcttca ggcaagttca atgacaattt caacatcatt gcagcagaca agatagtggc
     4621 gatagggttg accttattct ttggcaaatc tggagcagaa ccgtggcatg gttcgtacaa
     4681 accaaatgcg gtgttcttgt ctggcaaaga ggccaaggac gcagatggca acaaacccaa
     4741 ggaacctggg ataacggagg cttcatcgga gatgatatca ccaaacatgt tgctggtgat
     4801 tataatacca tttaggtggg ttgggttctt aactaggatc atggcggcag aatcaatcaa
     4861 ttgatgttga accttcaatg taggaaattc gttcttgatg gtttcctcca cagtttttct
     4921 ccataatctt gaagaggcca aaacattagc tttatccaag gaccaaatag gcaatggtgg
     4981 ctcatgttgt agggccatga aagcggccat tcttgtgatt ctttgcactt ctggaacggt
     5041 gtattgttca ctatcccaag cgacaccatc accatcgtct tcctttctct taccaaagta
     5101 aatacctccc actaattctc tgacaacaac gaagtcagta cctttagcaa attgtggctt
     5161 gattggagat aagtctaaaa gagagtcgga tgcaaagtta catggtctta agttggcgta
     5221 caattgaagt tctttacgga tttttagtaa accttgttca ggtctaacac tacctgtacc
     5281 ccatttagga ccacccacag cacctaacaa aacggcatca gccttcttgg aggcttccag
     5341 cgcctcatct ggaagtggga cacctgtagc ttcgatagca gcaccaccaa ttaaatgatt
     5401 ttcgaaatcg aacttgacat tggaacgaac atcagaaata gctttaagaa ccttaatggc
     5461 ttcggctgtg atttcttgac caacgtggtc acctggcaaa acgacgatct tcttaggggc
     5521 agacattaga atggtatatc cttgaaatat atatatatat tgctgaaatg taaaaggtaa
     5581 gaaaagttag aaagtaagac gattgctaac cacctattgg aaaaaacaat aggtccttaa
     5641 ataatattgt caacttcaag tattgtgatg caagcattta gtcatgaacg cttctctatt
     5701 ctatatgaaa agccggttcc ggcgctctca cctttccttt ttctcccaat ttttcagttg
     5761 aaaaaggtat atgcgtcagg cgacctctga aattaacaaa aaatttccag tcatcgaatt
     5821 tgattctgtg cgatagcgcc cctgtgtgtt ctcgttatgt tgaggaaaaa aataatggtt
     5881 gctaagagat tcgaactctt gcatcttacg atacctgagt attcccacag ttgggggatc
     5941 tcgactctag ctagaggatc aattcgtaat catgtcatag ctgtttcctg tgtgaaattg
     6001 ttatccgctc acaattccac acaacatacg agccggaagc ataaagtgta aagcctgggg
     6061 tgcctaatga gtgagctaac tcacattaat tgcgttgcgc tcactgcccg ctttccagtc
     6121 gggaaacctg tcgtgccagc tgataacttc gtataatgta tgctatacga agttattagg
     6181 tctgaagagg agtttacgtc cagccaagct agcttggctg caggtcgagc ggccgcgatc
     6241 cggaaccctt aatataactt cgtataatgt atgctatacg aagttatcag ctgcattaat
     6301 gaatcggcca acgcgcgggg agaggcggtt tgcgtattgg gcgctcttcc gcttcctcgc
     6361 tcactgactc gctgcgctcg gtcgttcggc tgcggcgagc ggtatcagct cactcaaagg
     6421 cggtaatacg gttatccaca gaatcagggg ataacgcagg aaagaacatg tgagcaaaag
     6481 gccagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc
     6541 gcccccctga cgagcatcac aaaaatcgac gctcaagtca gaggtggcga aacccgacag
     6601 gactataaag ataccaggcg tttccccctg gaagctccct cgtgcgctct cctgttccga
     6661 ccctgccgct taccggatac ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc
     6721 atagctcacg ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg
     6781 tgcacgaacc ccccgttcag cccgaccgct gcgccttatc cggtaactat cgtcttgagt
     6841 ccaacccggt aagacacgac ttatcgccac tggcagcagc cactggtaac aggattagca
     6901 gagcgaggta tgtaggcggt gctacagagt tcttgaagtg gtggcctaac tacggctaca
     6961 ctagaagaac agtatttggt atctgcgctc tgctgaagcc agttaccttc ggaaaaagag
     7021 ttggtagctc ttgatccggc aaacaaacca ccgctggtag cggtggtttt tttgtttgca
     7081 agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg
     7141 ggtctgacgc tcagtggaac gaaaactcac gttaagggat tttggtcatg agattatcaa
     7201 aaaggatctt cacctagatc cttttaaatt aaaaatgaag ttttaaatca atctaaagta
     7261 tatatgagta aacttggtct gacagttacc aatgcttaat cagtgaggca cctatctcag
     7321 cgatctgtct atttcgttca tccatagttg cctgactccc cgtcgtgtag ataactacga
     7381 tacgggaggg cttaccatct ggccccagtg ctgcaatgat accgcgagac ccacgctcac
     7441 cggctccaga tttatcagca ataaaccagc cagccggaag ggccgagcgc agaagtggtc
     7501 ctgcaacttt atccgcctcc atccagtcta ttaattgttg ccgggaagct agagtaagta
     7561 gttcgccagt taatagtttg cgcaacgttg ttgccattgc tacaggcatc gtggtgtcac
     7621 gctcgtcgtt tggtatggct tcattcagct ccggttccca acgatcaagg cgagttacat
     7681 gatcccccat gttgtgcaaa aaagcggtta gctccttcgg tcctccgatc gttgtcagaa
     7741 gtaagttggc cgcagtgtta tcactcatgg ttatggcagc actgcataat tctcttactg
     7801 tcatgccatc cgtaagatgc ttttctgtga ctggtgagta ctcaaccaag tcattctgag
     7861 aatagtgtat gcggcgaccg agttgctctt gcccggcgtc aatacgggat aataccgcgc
     7921 cacatagcag aactttaaaa gtgctcatca ttggaaaacg ttcttcgggg cgaaaactct
     7981 caaggatctt accgctgttg agatccagtt cgatgtaacc cactcgtgca cccaactgat
     8041 cttcagcatc ttttactttc accagcgttt ctgggtgagc aaaaacagga aggcaaaatg
     8101 ccgcaaaaaa gggaataagg gcgacacgga aatgttgaat actcatactc ttcctttttc
     8161 aatattattg aagcatttat cagggttatt gtctcatgag cggatacata tttgaatgta
     8221 tttagaaaaa taaacaaata ggggttccgc gcacatttcc ccgaaaagtg ccacctgacg
     8281 tctaagaaac cattattatc atgacattaa cctataaaaa taggcgtatc acgaggccct
     8341 ttcgtctcgc gcgtttcggt gatgacggtg aaaacctctg acacatgcag ctcccggaga
     8401 cggtcacagc ttgtctgtaa gcggatgccg ggagcagaca agcccgtcag ggcgcgtcag
     8461 cgggtgttgg cgggtgtcgg ggctggctta actatgcggc atcagagcag attgtactga
     8521 gagtgcacca taacgcattt aagcataaac acgcactatg ccgttcttct catgtatata
     8581 tatatacagg caacacgcag atataggtgc gacgtgaaca gtgagctgta tgtgcgcagc
     8641 tcgcgttgca ttttcggaag cgctcgtttt cggaaacgct ttgaagttcc tattccgaag
     8701 ttcctattct ctagctagaa agtataggaa cttcagagcg cttttgaaaa ccaaaagcgc
     8761 tctgaagacg cactttcaaa aaaccaaaaa cgcaccggac tgtaacgagc tactaaaata
     8821 ttgcgaatac cgcttccaca aacattgctc aaaagtatct ctttgctata tatctctgtg
     8881 ctatatccct atataaccta cccatccacc tttcgctcct tgaacttgca tctaaactcg
     8941 acctctacat tttttatgtt tatctctagt attactcttt agacaaaaaa attgtagtaa
     9001 gaactattca tagagtgaat cgaaaacaat acgaaaatgt aaacatttcc tatacgtagt
     9061 atatagagac aaaatagaag aaaccgttca taattttctg accaatgaag aatcatcaac
     9121 gctatcactt tctgttcaca aagtatgcgc aatccacatc ggtatagaat ataatcgggg
     9181 atgcctttat cttgaaaaaa tgcacccgca gcttcgctag taatcagtaa acgcgggaag
     9241 tggagtcagg ctttttttat ggaagagaaa atagacacca aagtagcctt cttctaacct
     9301 taacggacct acagtgcaaa aagttatcaa gagactgcat tatagagcgc acaaaggaga
     9361 aaaaaagtaa tctaagatgc tttgttagaa aaatagcgct ctcgggatgc atttttgtag
     9421 aacaaaaaag aagtatagat tctttgttgg taaaatagcg ctctcgcgtt gcatttctgt
     9481 tctgtaaaaa tgcagctcag attctttgtt tgaaaaatta gcgctctcgc gttgcatttt
     9541 tgttttacaa aaatgaagca cagattcttc gttggtaaaa tagcgctttc gcgttgcatt
     9601 tctgttctgt aaaaatgcag ctcagattct ttgtttgaaa aattagcgct ctcgcgttgc
     9661 atttttgttc tacaaaatga agcacagatg cttcgttgct
//
