LOCUS       sequence-496344-        3102 bp    DNA     circular SYN 07-JAN-2026
DEFINITION  PhlF part vector for Golden Gate Assembly.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3102)
  TITLE     Tropini Lab plasmids
REFERENCE   2  (bases 1 to 3102)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Jan 7, 2026 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pEM118
FEATURES             Location/Qualifiers
     source          1..3102
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      complement(35..77)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     terminator      910..981
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     terminator      997..1024
                     /label=T7Te terminator
                     /note="phage T7 early transcription terminator"
     primer_bind     complement(1124..1141)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(1295..1883)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(1375..1394)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     terminator      complement(2066..2160)
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     CDS             complement(2181..2840)
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     2755..2774
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     primer_bind     3002..3020
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     terminator      complement(3024..3067)
                     /label=bacterial terminator
                     /note="putative bacterial transcription terminator"
     misc_feature    3070..3089
                     /label=BioBrick prefix
                     /note="BioBrick prefix for parts that start with ""ATG"""
ORIGIN
        1 gttcctcgat tcagatctaa aggatccaat ctagataaaa cgaaaggctc agtcgaaaga
       61 ctgggccttt cgttttacaa ttgggctacc ttttttttgt tttgtttgca atggttaatc
      121 tattgttaaa atttaaagtt tcacttgaac tttcaaataa tgttcttata tttccagtgt
      181 cgaaagaaac aaagtaggac tgatcacaca acatttaaaa aataacatta tgaaagcact
      241 cgaaaagaga ttcaaaggta atattaacaa taatttattt tcaatggcac gtaccccgag
      301 ccgtagcagc attggtagcc tgcgtagtcc gcatacccat aaagcaattc tgaccagcac
      361 cattgaaatc ctgaaagaat gtggttatag cggtctgagc attgaaagcg ttgcacgtcg
      421 tgccggtgca agcaaaccga ccatttatcg ttggtggacc aataaagcag cactgattgc
      481 cgaagtgtat gaaaatgaaa gcgaacaggt gcgtaaattt ccggatctgg gtagctttaa
      541 agccgatctg gattttctgc tgcgtaatct gtggaaagtt tggcgtgaaa ccatttgtgg
      601 tgaagcattt cgttgtgtta ttgcagaagc acagctggac cctgcaaccc tgacccagct
      661 gaaagatcag tttatggaac gtcgtcgtga gatgccgaaa aaactggttg aaaatgccat
      721 tagcaatggt gaactgccga aagataccaa tcgtgaactg ctgctggata tgatttttgg
      781 tttttgttgg tatcgcctgc tgaccgaaca gctgaccgtt gaacaggata ttgaagaatt
      841 taccttcctg ctgattaatg gtgtttgtcc gggtacacag cgttaactag ggcccatacc
      901 cccaggcatc aaataaaacg aaaggctcag tcgaaagact gggcctttcg ttttatctgt
      961 tgtttgtcgg tgaacgctct ctactagagt cacactggct caccttcggg tgggcctttc
     1021 tgcgtttata cgacgtacgg tggaatctga ttcgttacca attgacatga tacgaaacgt
     1081 accgtatcga cggtgagacc ttacttcgcg ttatgcaggc ttcctcgctc actgactcgc
     1141 tgcgctcggt cgttcggctg cggcgagcgg tatcagctca ctcaaaggcg gtaatacggt
     1201 tatccacaga atcaggggat aacgcaggaa agaacatgtg agcaaaaggc cagcaaaagg
     1261 ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca caggctccgc ccccctgacg
     1321 agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa cccgacagga ctataaagat
     1381 accaggcgtt tccccctgga agctccctcg tgcgctctcc tgttccgacc ctgccgctta
     1441 ccggatacct gtccgccttt ctcccttcgg gaagcgtggc gctttctcat agctcacgct
     1501 gtaggtatct cagttcggtg taggtcgttc gctccaagct gggctgtgtg cacgaacccc
     1561 ccgttcagcc cgaccgctgc gccttatccg gtaactatcg tcttgagtcc aacccggtaa
     1621 gacacgactt atcgccactg gcagcagcca ctggtaacag gattagcaga gcgaggtatg
     1681 taggcggtgc tacagagttc ttgaagtggt ggcctaacta cggctacact agaagaacag
     1741 tatttggtat ctgcgctctg ctgaagccag ttaccttcgg aaaaagagtt ggtagctctt
     1801 gatccggcaa acaaaccacc gctggtagcg gtggtttttt tgtttgcaag cagcagatta
     1861 cgcgcagaaa aaaaggatct caagaagatc ctttgatctt ttctacgggg tctgacgctc
     1921 agtggaacga aaactcacgt taagggattt tggtcatgag attatcaaaa aggatcttca
     1981 cctagatcct tttaaattaa aaatgaagtt ttaaatcaat ctaaagtata tatgagtaaa
     2041 cttggtctga cagctcgagg cttggattct caccaataaa aaacgcccgg cggcaaccga
     2101 gcgttctgaa caaatccaga tggagttctg aggtcattac tggatctatc aacaggagtc
     2161 caagcgagct cgatatcaaa ttacgccccg ccctgccact catcgcagta ctgttgtaat
     2221 tcattaagca ttctgccgac atggaagcca tcacaaacgg catgatgaac ctgaatcgcc
     2281 agcggcatca gcaccttgtc gccttgcgta taatatttgc ccatggtgaa aacgggggcg
     2341 aagaagttgt ccatattggc cacgtttaaa tcaaaactgg tgaaactcac ccagggattg
     2401 gctgacacga aaaacatatt ctcaataaac cctttaggga aataggccag gttttcaccg
     2461 taacacgcca catcttgcga atatatgtgt agaaactgcc ggaaatcgtc gtggtattca
     2521 ctccagagcg atgaaaacgt ttcagtttgc tcatggaaaa cggtgtaaca agggtgaaca
     2581 ctatcccata tcaccagctc accgtctttc attgccatac gaaattccgg atgagcattc
     2641 atcaggcggg caagaatgtg aataaaggcc ggataaaact tgtgcttatt tttctttacg
     2701 gtctttaaaa aggccgtaat atccagctga acggtctggt tataggtaca ttgagcaact
     2761 gactgaaatg cctcaaaatg ttctttacga tgccattggg atatatcaac ggtggtatat
     2821 ccagtgattt ttttctccat tttagcttcc ttagctcctg aaaatctcga taactcaaaa
     2881 aatacgcccg gtagtgatct tatttcatta tggtgaaagt tggaacctct tacgtgcccg
     2941 atcaactcga gtgccacctg acgtctaaga aaccattatt atcatgacat taacctataa
     3001 aaataggcgt atcacgaggc agaatttcag ataaaaaaaa tccttagctt tcgctaagga
     3061 tgatttctgg aattcgcggc cgcttctaga atctaggtct cc
//
