LOCUS       sequence-496354-        9016 bp    DNA     circular SYN 07-JAN-2026
DEFINITION  Empty vector for generating yeast 2-hybrid GAL4 DNA binding domain 
            fusion constructs by Gateway cloning. It may also be used as an 
            empty vector experimental control..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9016)
  AUTHORS   Rossignol P, Collier S, Bush M, Shaw P, Doonan JH
  TITLE     Arabidopsis POT1A interacts with TERT-V(I8), an N-terminal splicing 
            variant of telomerase.
  JOURNAL   J Cell Sci. 2007 Oct 15;120(Pt 20):3678-87. doi: 10.1242/jcs.004119.
            Epub 2007 Oct 2.
  PUBMED    17911168
REFERENCE   2  (bases 1 to 9016)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Jan 7, 2026 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Cell 
            Sci."; date: "2007-10-15"; volume: "120(Pt 20):3678-87. doi"; pages:
            " 10.1242/jcs.004119. Epub 2007 Oct 2"
COMMENT     Sequence Label: pDEST-GBKT7
FEATURES             Location/Qualifiers
     source          1..9016
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        30..734
                     /gene="S. cerevisiae ADH1"
                     /label=ADH1 promoter
                     /note="promoter for alcohol dehydrogenase 1"
     CDS             762..1202
                     /codon_start=1
                     /gene="Saccharomyces cerevisiae GAL4 (truncated)"
                     /product="DNA binding domain of the GAL4 transcriptional 
                     activator"
                     /label=GAL4 DNA binding domain
                     /translation="MKLLSSIEQACDICRLKKLKCSKEKPKCAKCLKNNWECRYSPKTK
                     RSPLTRAHLTEVESRLERLEQLFLLIFPREDLDMILKMDSLQDIKALLTGLFVQDNVNK
                     DAVTDRLASVETDMPLTLRQHRISATSSSEESSNKGQRQLTVS"
     primer_bind     1213..1232
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        1213..1231
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             1251..1280
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     protein_bind    1311..1435
                     /gene="mutant version of attR"
                     /label=attR1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     promoter        join(1460..1465,1466..1483,1484..1490)
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an ""up"" mutation"
     CDS             1544..2203
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     complement(1610..1629)
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     CDS             2545..2850
                     /codon_start=1
                     /gene="ccdB"
                     /product="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /label=ccdB
                     /note="Plasmids containing the ccdB gene cannot be 
                     propagated in standard E. coli strains."
                     /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK
                     VSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     primer_bind     2729..2748
                     /label=ccdB-fwd
                     /note="ccdB gene, forward primer"
     protein_bind    complement(2891..3015)
                     /gene="mutant version of attR"
                     /label=attR2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     terminator      3051..3098
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     terminator      3125..3312
                     /gene="S. cerevisiae ADH1"
                     /label=ADH1 terminator
                     /note="transcription terminator for the S. cerevisiae 
                     alcohol dehydrogenase 1 (ADH1) gene"
     primer_bind     complement(3180..3204)
                     /label=pHybLex-R
                     /note="ADH terminator, reverse primer"
     primer_bind     complement(3336..3352)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3336..3352)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(3349..3371)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    3360..3376
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(3384..3390,3391..3408,3409..3414))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    3429..3450
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(3567..3584)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(3738..4326)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(3818..3837)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     polyA_signal    4655..4702
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     primer_bind     4727..4746
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     CDS             complement(4934..5728)
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     CDS             complement(4934..5728)
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(5045..5064)
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     5655..5674
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     promoter        complement(5729..5833)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      5860..7202
                     /label=2u ori
                     /note="yeast 2u plasmid origin of replication"
     protein_bind    complement(6823..6870)
                     /label=FRT
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="FLP-mediated recombination occurs in the 8-bp core 
                     sequence TCTAGAAA (Turan and Bode, 2011)."
     primer_bind     7243..7261
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(7299..7321)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     7421..7440
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     promoter        7461..7742
                     /gene="S. cerevisiae TRP1"
                     /label=TRP1 promoter
     CDS             7743..8417
                     /codon_start=1
                     /gene="S. cerevisiae TRP1"
                     /product="phosphoribosylanthranilate isomerase, required 
                     for tryptophan biosynthesis"
                     /label=TRP1
                     /note="yeast auxotrophic marker"
                     /translation="MSVINFTGSSGPLVKVCGLQSTEAAECALDSDADLLGIICVPNRK
                     RTIDPVIARKISSLVKAYKNSSGTPKYLVGVFRNQPKEDVLALVNDYGIDIVQLHGDES
                     WQEYQEFLGLPVIKRLVFPKDCNILLSAASQKPHSFIPLFDSEAGGTGELLDWNSISDW
                     VGRQESPESLHFMLAGGLTPENVGDALRLNGVIGVDVSGGVETNGVKDSNKIANFVKNA
                     KK"
     rep_origin      complement(8520..8975)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(8657..8678)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     8869..8888
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
ORIGIN
        1 cggtgcgggc ctcttcgcta ttacgccaga tccttttgtt gtttccgggt gtacaatatg
       61 gacttcctct tttctggcaa ccaaacccat acatcgggat tcctataata ccttcgttgg
      121 tctccctaac atgtaggtgg cggaggggag atatacaata gaacagatac cagacaagac
      181 ataatgggct aaacaagact acaccaatta cactgcctca ttgatggtgg tacataacga
      241 actaatactg tagccctaga cttgatagcc atcatcatat cgaagtttca ctaccctttt
      301 tccatttgcc atctattgaa gtaataatag gcgcatgcaa cttcttttct ttttttttct
      361 tttctctctc ccccgttgtt gtctcaccat atccgcaatg acaaaaaaat gatggaagac
      421 actaaaggaa aaaattaacg acaaagacag caccaacaga tgtcgttgtt ccagagctga
      481 tgaggggtat ctcgaagcac acgaaacttt ttccttcctt cattcacgca cactactctc
      541 taatgagcaa cggtatacgg ccttccttcc agttacttga atttgaaata aaaaaaagtt
      601 tgctgtcttg ctatcaagta taaatagacc tgcaattatt aatcttttgt ttcctcgtca
      661 ttgttctcgt tccctttctt ccttgtttct ttttctgcac aatatttcaa gctataccaa
      721 gcatacaatc aactccaagc ttgaagcaag cctcctgaaa gatgaagcta ctgtcttcta
      781 tcgaacaagc atgcgatatt tgccgactta aaaagctcaa gtgctccaaa gaaaaaccga
      841 agtgcgccaa gtgtctgaag aacaactggg agtgtcgcta ctctcccaaa accaaaaggt
      901 ctccgctgac tagggcacat ctgacagaag tggaatcaag gctagaaaga ctggaacagc
      961 tatttctact gatttttcct cgagaagacc ttgacatgat tttgaaaatg gattctttac
     1021 aggatataaa agcattgtta acaggattat ttgtacaaga taatgtgaat aaagatgccg
     1081 tcacagatag attggcttca gtggagactg atatgcctct aacattgaga cagcatagaa
     1141 taagtgcgac atcatcatcg gaagagagta gtaacaaagg tcaaagacag ttgactgtat
     1201 cgccggaatt tgtaatacga ctcactatag ggcgagccgc catcatggag gagcagaagc
     1261 tgatctcaga ggaggacctg catatggcca tggaggccga attcccatca acaagtttgt
     1321 acaaaaaagc tgaacgagaa acgtaaaatg atataaatat caatatatta aattagattt
     1381 tgcataaaaa acagactaca taatactgta aaacacaaca tatccagtca ctatggcggc
     1441 cgcattaggc accccaggct ttacacttta tgcttccggc tcgtataatg tgtggatttt
     1501 gagttaggat ccgtcgagat tttcaggagc taaggaagct aaaatggaga aaaaaatcac
     1561 tggatatacc accgttgata tatcccaatg gcatcgtaaa gaacattttg aggcatttca
     1621 gtcagttgct caatgtacct ataaccagac cgttcagctg gatattacgg cctttttaaa
     1681 gaccgtaaag aaaaataagc acaagtttta tccggccttt attcacattc ttgcccgcct
     1741 gatgaatgct catccggaat tccgtatggc aatgaaagac ggtgagctgg tgatatggga
     1801 tagtgttcac ccttgttaca ccgttttcca tgagcaaact gaaacgtttt catcgctctg
     1861 gagtgaatac cacgacgatt tccggcagtt tctacacata tattcgcaag atgtggcgtg
     1921 ttacggtgaa aacctggcct atttccctaa agggtttatt gagaatatgt ttttcgtctc
     1981 agccaatccc tgggtgagtt tcaccagttt tgatttaaac gtggccaata tggacaactt
     2041 cttcgccccc gttttcacca tgggcaaata ttatacgcaa ggcgacaagg tgctgatgcc
     2101 gctggcgatt caggttcatc atgccgtttg tgatggcttc catgtcggca gaatgcttaa
     2161 tgaattacaa cagtactgcg atgagtggca gggcggggcg taaagatctg gatccggctt
     2221 actaaaagcc agataacagt atgcgtattt gcgcgctgat ttttgcggta taagaatata
     2281 tactgatatg tatacccgaa gtatgtcaaa aagaggtatg ctatgaagca gcgtattaca
     2341 gtgacagttg acagcgacag ctatcagttg ctcaaggcat atatgatgtc aatatctccg
     2401 gtctggtaag cacaaccatg cagaatgaag cccgtcgtct gcgtgccgaa cgctggaaag
     2461 cggaaaatca ggaagggatg gctgaggtcg cccggtttat tgaaatgaac ggctcttttg
     2521 ctgacgagaa caggggctgg tgaaatgcag tttaaggttt acacctataa aagagagagc
     2581 cgttatcgtc tgtttgtgga tgtacagagt gatattattg acacgcccgg gcgacggatg
     2641 gtgatccccc tggccagtgc acgtctgctg tcagataaag tctcccgtga actttacccg
     2701 gtggtgcata tcggggatga aagctggcgc atgatgacca ccgatatggc cagtgtgccg
     2761 gtctccgtta tcggggaaga agtggctgat ctcagccacc gcgaaaatga catcaaaaac
     2821 gccattaacc tgatgttctg gggaatataa atgtcaggct cccttataca cagccagtct
     2881 gcaggtcgac catagtgact ggatatgttg tgttttacag tattatgtag tctgtttttt
     2941 atgcaaaatc taatttaata tattgatatt tatatcattt tacgtttctc gttcagcttt
     3001 cttgtacaaa gtggttgatg gggatccgtc gacctgcagc ggccgcataa ctagcataac
     3061 cccttggggc ctctaaacgg gtcttgaggg gttttttgcg cgcttgcagc caagctaatt
     3121 ccgggcgaat ttcttatgat ttatgatttt tattattaaa taagttataa aaaaaataag
     3181 tgtatacaaa ttttaaagtg actcttaggt tttaaaacga aaattcttat tcttgagtaa
     3241 ctctttcctg taggtcaggt tgctttctca ggtatagcat gaggtcgctc ttattgacca
     3301 cacctctacc ggcatgcaag cttggcgtaa tcatggtcat agctgtttcc tgtgtgaaat
     3361 tgttatccgc tcacaattcc acacaacata cgagccggaa gcataaagtg taaagcctgg
     3421 ggtgcctaat gagtgagcta actcacatta attgcgttgc gctcactgcc cgctttccag
     3481 tcgggaaacc tgtcgtgcca gctgcattaa tgaatcggcc aacgcgcggg gagaggcggt
     3541 ttgcgtattg ggcgctcttc cgcttcctcg ctcactgact cgctgcgctc ggtcgttcgg
     3601 ctgcggcgag cggtatcagc tcactcaaag gcggtaatac ggttatccac agaatcaggg
     3661 gataacgcag gaaagaacat gtgagcaaaa ggccagcaaa aggccaggaa ccgtaaaaag
     3721 gccgcgttgc tggcgttttt ccataggctc cgcccccctg acgagcatca caaaaatcga
     3781 cgctcaagtc agaggtggcg aaacccgaca ggactataaa gataccaggc gtttccccct
     3841 ggaagctccc tcgtgcgctc tcctgttccg accctgccgc ttaccggata cctgtccgcc
     3901 tttctccctt cgggaagcgt ggcgctttct catagctcac gctgtaggta tctcagttcg
     3961 gtgtaggtcg ttcgctccaa gctgggctgt gtgcacgaac cccccgttca gcccgaccgc
     4021 tgcgccttat ccggtaacta tcgtcttgag tccaacccgg taagacacga cttatcgcca
     4081 ctggcagcag ccactggtaa caggattagc agagcgaggt atgtaggcgg tgctacagag
     4141 ttcttgaagt ggtggcctaa ctacggctac actagaagaa cagtatttgg tatctgcgct
     4201 ctgctgaagc cagttacctt cggaaaaaga gttggtagct cttgatccgg caaacaaacc
     4261 accgctggta gcggtggttt ttttgtttgc aagcagcaga ttacgcgcag aaaaaaagga
     4321 tctcaagaag atcctttgat cttttctacg gggtctgacg ctcagtggaa cgaaaactca
     4381 cgttaaggga ttttggtcat gagattatca aaaaggatct tcacctagat ccttttaaat
     4441 taaaaatgaa gttttaaatc aatctaaagt atatatgagt aacctgaggc tatggcaggg
     4501 cctgccgccc cgacgttggc tgcgagccct gggccttcac ccgaacttgg ggggtggggt
     4561 ggggaaaagg aagaaacgcg ggcgtattgg ccccaatggg gtctcggtgg ggtatcgaca
     4621 gagtgccagc cctgggaccg aaccccgcgt ttatgaacaa acgacccaac accgtgcgtt
     4681 ttattctgtc tttttattgc cgtcatagcg cgggttcctt ccggtattgt ctccttccgt
     4741 gtttcagtta gcctccccct agggtgggcg aagaactcca gcatgagatc cccgcgctgg
     4801 aggatcatcc agccggcgtc ccggaaaacg attccgaagc ccaacctttc atagaaggcg
     4861 gcggtggaat cgaaatctcg tgatggcagg ttgggcgtcg cttggtcggt catttcgaac
     4921 cccagagtcc cgctcagaag aactcgtcaa gaaggcgata gaaggcgatg cgctgcgaat
     4981 cgggagcggc gataccgtaa agcacgagga agcggtcagc ccattcgccg ccaagctctt
     5041 cagcaatatc acgggtagcc aacgctatgt cctgatagcg gtccgccaca cccagccggc
     5101 cacagtcgat gaatccagaa aagcggccat tttccaccat gatattcggc aagcaggcat
     5161 cgccatgagt cacgacgaga tcctcgccgt cgggcatgct cgccttgagc ctggcgaaca
     5221 gttcggctgg cgcgagcccc tgatgctctt cgtccagatc atcctgatcg acaagaccgg
     5281 cttccatccg agtacgtgct cgctcgatgc gatgtttcgc ttggtggtcg aatgggcagg
     5341 tagccggatc aagcgtatgc agccgccgca ttgcatcagc catgatggat actttctcgg
     5401 caggagcaag gtgagatgac aggagatcct gccccggcac ttcgcccaat agcagccagt
     5461 cccttcccgc ttcagtgaca acgtcgagca cagctgcgca aggaacgccc gtcgtggcca
     5521 gccacgatag ccgcgctgcc tcgtcttgca gttcattcag ggcaccggac aggtcggtct
     5581 tgacaaaaag aaccgggcgc ccctgcgctg acagccggaa cacggcggca tcagagcagc
     5641 cgattgtctg ttgtgcccag tcatagccga atagcctctc cacccaagcg gccggagaac
     5701 ctgcgtgcaa tccatcttgt tcaatcatac tcttcctttt tcaatattat tgaagcattt
     5761 atcagggtta ttgtctcatg agcggataca tatttgaatg tatttagaaa aataaacaaa
     5821 taggggttcc gcgcacattt ccccgaaaag tgccacctga acgaagcatc tgtgcttcat
     5881 tttgtagaac aaaaatgcaa cgcgagagcg ctaatttttc aaacaaagaa tctgagctgc
     5941 atttttacag aacagaaatg caacgcgaaa gcgctatttt accaacgaag aatctgtgct
     6001 tcatttttgt aaaacaaaaa tgcaacgcga gagcgctaat ttttcaaaca aagaatctga
     6061 gctgcatttt tacagaacag aaatgcaacg cgagagcgct attttaccaa caaagaatct
     6121 atacttcttt tttgttctac aaaaatgcat cccgagagcg ctatttttct aacaaagcat
     6181 cttagattac tttttttctc ctttgtgcgc tctataatgc agtctcttga taactttttg
     6241 cactgtaggt ccgttaaggt tagaagaagg ctactttggt gtctattttc tcttccataa
     6301 aaaaagcctg actccacttc ccgcgtttac tgattactag cgaagctgcg ggtgcatttt
     6361 ttcaagataa aggcatcccc gattatattc tataccgatg tggattgcgc atactttgtg
     6421 aacagaaagt gatagcgttg atgattcttc attggtcaga aaattatgaa cggtttcttc
     6481 tattttgtct ctatatacta cgtataggaa atgtttacat tttcgtattg ttttcgattc
     6541 actctatgaa tagttcttac tacaattttt ttgtctaaag agtaatacta gagataaaca
     6601 taaaaaatgt agaggtcgag tttagatgca agttcaagga gcgaaaggtg gatgggtagg
     6661 ttatataggg atatagcaca gagatatata gcaaagagat acttttgagc aatgtttgtg
     6721 gaagcggtat tcgcaatatt ttagtagctc gttacagtcc ggtgcgtttt tggttttttg
     6781 aaagtgcgtc ttcagagcgc ttttggtttt caaaagcgct ctgaagttcc tatactttct
     6841 agagaatagg aacttcggaa taggaacttc aaagcgtttc cgaaaacgag cgcttccgaa
     6901 aatgcaacgc gagctgcgca catacagctc actgttcacg tcgcacctat atctgcgtgt
     6961 tgcctgtata tatatataca tgagaagaac ggcatagtgc gtgtttatgc ttaaatgcgt
     7021 acttatatgc gtctatttat gtaggatgaa aggtagtcta gtacctcctg tgatattatc
     7081 ccattccatg cggggtatcg tatgcttcct tcagcactac cctttagctg ttctatatgc
     7141 tgccactcct caattggatt agtctcatcc ttcaatgcta tcatttcctt tgatattgga
     7201 tcatactaag aaaccattat tatcatgaca ttaacctata aaaataggcg tatcacgagg
     7261 ccctttcgtc tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg
     7321 gagacggtca cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg
     7381 tcagcgggtg ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta
     7441 ctgagagtgc accatagatc aacgacatta ctatatatat aatataggaa gcatttaata
     7501 gaacagcatc gtaatatatg tgtactttgc agttatgacg ccagatggca gtagtggaag
     7561 atattcttta ttgaaaaata gcttgtcacc ttacgtacaa tcttgatccg gagcttttct
     7621 ttttttgccg attaagaatt aattcggtcg aaaaaagaaa aggagagggc caagagggag
     7681 ggcattggtg actattgagc acgtgagtat acgtgattaa gcacacaaag gcagcttgga
     7741 gtatgtctgt tattaatttc acaggtagtt ctggtccatt ggtgaaagtt tgcggcttgc
     7801 agagcacaga ggccgcagaa tgtgctctag attccgatgc tgacttgctg ggtattatat
     7861 gtgtgcccaa tagaaagaga acaattgacc cggttattgc aaggaaaatt tcaagtcttg
     7921 taaaagcata taaaaatagt tcaggcactc cgaaatactt ggttggcgtg tttcgtaatc
     7981 aacctaagga ggatgttttg gctctggtca atgattacgg cattgatatc gtccaactgc
     8041 atggagatga gtcgtggcaa gaataccaag agttcctcgg tttgccagtt attaaaagac
     8101 tcgtatttcc aaaagactgc aacatactac tcagtgcagc ttcacagaaa cctcattcgt
     8161 ttattccctt gtttgattca gaagcaggtg ggacaggtga acttttggat tggaactcga
     8221 tttctgactg ggttggaagg caagagagcc ccgaaagctt acattttatg ttagctggtg
     8281 gactgacgcc agaaaatgtt ggtgatgcgc ttagattaaa tggcgttatt ggtgttgatg
     8341 taagcggagg tgtggagaca aatggtgtaa aagactctaa caaaatagca aatttcgtca
     8401 aaaatgctaa gaaataggtt attactgagt agtatttatt taagtattgt ttgtgcactt
     8461 gccgatctat gcggtgtgaa ataccgcaca gatgcgtaag gagaaaatac cgcatcagga
     8521 aattgtaagc gttaatattt tgttaaaatt cgcgttaaat ttttgttaaa tcagctcatt
     8581 ttttaaccaa taggccgaaa tcggcaaaat cccttataaa tcaaaagaat agaccgagat
     8641 agggttgagt gttgttccag tttggaacaa gagtccacta ttaaagaacg tggactccaa
     8701 cgtcaaaggg cgaaaaaccg tctatcaggg cgatggccca ctacgtgaac catcacccta
     8761 atcaagtttt ttggggtcga ggtgccgtaa agcactaaat cggaacccta aagggagccc
     8821 ccgatttaga gcttgacggg gaaagccggc gaacgtggcg agaaaggaag ggaagaaagc
     8881 gaaaggagcg ggcgctaggg cgctggcaag tgtagcggtc acgctgcgcg taaccaccac
     8941 acccgccgcg cttaatgcgc cgctacaggg cgcgtccatt cgccattcag gctgcgcaac
     9001 tgttgggaag ggcgat
//
