LOCUS       sequence-496361-        7324 bp    DNA     circular SYN 07-JAN-2026
DEFINITION  Mammalian expression vector of talin1 C-terminal fragment fused with
            pdDronpa1.2.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7324)
  AUTHORS   Nishimura R, Barnett SFH, Jain K, Huang Z, Goult BT, Kanchanawong P
  TITLE     Optogenetic control of mechanotransduction based on light-induced 
            homodimerization of talin.
  JOURNAL   J Cell Sci. 2025 Nov 24:jcs.264110. doi: 10.1242/jcs.264110.
  PUBMED    41277405
REFERENCE   2  (bases 1 to 7324)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Jan 7, 2026 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Cell Sci.
            2025 Nov 24:jcs.264110. doi: 10.1242/jcs.264110."
COMMENT     Sequence Label: pdDronpa1.2-Talin1(1974–2541)-mIFP
FEATURES             Location/Qualifiers
     source          1..7324
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        61..364
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        365..568
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     519..539
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     CDS             3031..3990
                     /codon_start=1
                     /product="naturally monomeric infrared fluorescent protein 
                     derived from a truncated bacteriophytochrome (Yu et al., 
                     2015)"
                     /label=mIFP
                     /translation="MSVPLTTSAFGHAFLANCEREQIHLAGSIQPHGILLAVKEPDNVV
                     IQASINAAEFLNTNSVVGRPLRDLGGDLPLQILPHLNGPLHLAPMTLRCTVGSPPRRVD
                     CTIHRPSNGGLIVELEPATKTTNIAPALDGAFHRITSSSSLMGLCDETATIIREITGYD
                     RVMVVRFDEEGNGEILSERRRADLEAFLGNRYPASTIPQIARRLYEHNRVRLLVDVNYT
                     PVPLQPRISPLNGRDLDMSLSCLRSMSPIHQKYMQDMGVGATLVCSLMVSGRLWGLIAC
                     HHYEPRFVPFHIRAAGEALAETCAIRIATLESFAQSQSK"
     polyA_signal    4112..4233
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(4149..4168)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     4203..4222
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      complement(4240..4695)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(4377..4398)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     4589..4608
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     promoter        4722..4826
                     /gene="bla"
                     /label=AmpR promoter
     promoter        4828..5185
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     enhancer        4828..5019
                     /label=SV40 enhancer
                     /note="enhancer for the SV40 early promoter (Herr, 1993)"
     primer_bind     complement(4851..4871)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     rep_origin      5036..5171
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             5220..6014
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     CDS             5220..6014
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(5274..5293)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     5884..5903
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     complement(6202..6221)
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     polyA_signal    6246..6293
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     rep_origin      6622..7210
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     7111..7130
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata tggagttccg
       61 cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt
      121 gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca
      181 atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc
      241 aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta
      301 catgacctta tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac
      361 catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg actcacgggg
      421 atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg
      481 ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg gtaggcgtgt
      541 acggtgggag gtctatataa gcagagctgg tttagtgaac cgtcagatcc gctagccacc
      601 atgtccgtga ttaaaccaga catgaagatc aagctgcgta tggaaggcgc tgtaaatgga
      661 cacccgttcg cgattgaagg agttggcctt gggaagcctt tcgagggaaa acagagtatt
      721 gaccttaaag tcaaagaagg cggacctctg cctttcgcct atgacatctt gacaactgct
      781 ttctgttacg gcaacagggt attcgccaaa tacccagaaa atatagtaga ctatttcaag
      841 cagtcgtttc ctgagggcta ctcttgggaa cgaagcatga attacgaaga cgggggcatt
      901 tgtaacgcga caaacgacat aaccctggat ggtgactgtt atatctatga aattcgattt
      961 agaggtacga actttcctgc caatggtcca gttatgcaga agaggactgt gaaatgggag
     1021 ccatccactg agaacttgta tgtgcgtgat ggagtgctga agggtgatgt tatcatggct
     1081 ctgtcgcttg aaggaggtgg ccattaccga tgtgacttca aaactactta taaagctaag
     1141 aaggttgtcc agttgccaga ctatcacttt gtggaccacc acattgagat taaaagccac
     1201 gacaaagatt acagtaatgt taatctgcat gagcacgccg aagcgcattc tgagctgccg
     1261 aggcagggtg gttctggtgg tagcactagt gatatcggta cccaggcctg cattacagca
     1321 gccagtgctg tgtctggtat cattgctgac ctcgacacca ccatcatgtt cgctactgct
     1381 ggcacactta accgtgaggg tgctgaaact tttgctgacc accgggaggg tatcttaaag
     1441 acagcaaagg ttcttgtgga ggacaccaag gtcctagtgc agaatgcagc tgggagccag
     1501 gagaagttgg cacaagccgc ccagtcctcc gtggccacca ttacccgcct cgctgatgtg
     1561 gtcaagctcg gtgcagccag cctaggagcc gaagaccctg aaactcaggt ggtgctgatc
     1621 aatgcagtaa aggacgtagc caaggccctg ggtgacctca tcagcgctac gaaggctgca
     1681 gcgggcaaag ttggggatga ccctgcagtg tggcagctca agaactctgc caaggtgatg
     1741 gtgaccaatg tgacatcatt gctcaagaca gtgaaggctg tggaagatga ggccaccaaa
     1801 ggcacacggg ccctagaggc aaccacagag cacatacgtc aggaactggc ggtcttctgt
     1861 tccccagagc cacctgccaa gacctctacc cctgaagact tcatccgaat gaccaagggt
     1921 attactatgg caacagccaa agccgttgct gctggcaatt cctgtcgaca ggaagatgtc
     1981 attgccacag ccaatctgag ccgacgggct attgcggaca tgcttcgggc ttgcaaggaa
     2041 gcagctttcc acccagaagt ggcgcctgat gtacggctcc gagccctgca ctatggccgg
     2101 gagtgtgcca atggttacct ggaactgctg gaccacgtgc tgctgaccct tcagaagcca
     2161 aacccagacc tgaagcagca gctaactgga cactcaaagc gggttgctgg ctctgtgact
     2221 gagctcatcc aggctgctga agccatgaaa ggaacagagt gggtggaccc agaggaccct
     2281 actgtcattg ctgagaatga gctcttggga gccgcagccg ccatcgaggc tgcagccaag
     2341 aagctggagc agctgaagcc cagggccaaa cccaaggagg cggatgagtc cttgaacttt
     2401 gaggaacaaa tcctagaagc tgccaagtcc atcgctgcag ccaccagtgc actggtaaag
     2461 gctgcgtcag cagcccagag ggagctggtg gctcaaggaa aggtgggcgc cattccagcc
     2521 aatgcactgg atgatgggca gtggtcgcag ggcctcattt ccgctgcccg tatggtggct
     2581 gcggccacca acaatctgtg tgaagcagct aacgcagctg tccagggcca tgctagccag
     2641 gagaaactca tttcctcagc caagcaagta gctgcctcca cagctcagct cctggtagct
     2701 tgcaaggtca aagcagatca ggactctgag gcaatgaaac ggctccaggc tgctggcaat
     2761 gcagtgaaga gggcctcaga taacctggta aaggcggccc agaaagctgc agccttcgaa
     2821 gaccaggaga atgagacggt ggtggtgaag gagaaaatgg ttgggggcat tgcccagatt
     2881 atcgcagcac aggaagagat gcttcggaag gaacgagagc tggaagaggc tcggaaaaag
     2941 ctcgcccaga tccggcagca gcagtacaag ttcttgcctt cagagcttcg agacgagcac
     3001 ggctccgcgt cccgatcacc ggtcgccacc atgtcggtac cgctgactac ctcagcattc
     3061 ggccacgcgt ttctggctaa ctgtgaacgc gagcagatcc acctggcggg ctccattcag
     3121 ccgcacggta tcctgctggc tgtgaaagag ccggacaacg tggtgatcca ggcttctatt
     3181 aacgctgcgg agttcctgaa caccaactct gttgttggcc gtccgctgcg tgacctgggc
     3241 ggcgatctgc ctttgcagat cctgccgcac ctgaacggcc cgctgcacct ggctccgatg
     3301 accctgcgtt gtaccgtggg ttctccgccg cgtcgtgtgg actgtaccat tcatcgtccg
     3361 tctaacggcg gcctgatcgt agaactggaa ccagcaacca agaccactaa cattgcgccg
     3421 gctctggacg gtgcgtttca tcgtatcact tcttcatcct ccctgatggg cctgtgtgac
     3481 gaaaccgcga ctattatccg tgagattact ggctacgacc gtgtgatggt agtacgtttc
     3541 gatgaagagg gtaatggcga aattctgtcc gaacgtcgtc gtgcggacct ggaagcgttc
     3601 ctgggtaacc gctacccggc gtctactatt ccgcagatcg ctcgtcgcct gtacgaacat
     3661 aaccgtgttc gcctgctggt agatgtgaac tatactccgg ttccgctaca gccgcgcatc
     3721 agcccgctga acggtcgtga tctggatatg tccctgtctt gcctgcgctc tatgtccccg
     3781 atccaccaga aatacatgca ggacatgggc gttggcgcga ccctggtttg ctctctgatg
     3841 gtgtctggtc gtctgtgggg tctgatcgct tgccaccact acgaaccgcg cttcgttccg
     3901 ttccacattc gcgctgctgg cgaagcgctg gcggaaactt gtgcgatccg catcgcgacg
     3961 ctggagagct ttgcacagtc tcagtccaaa gcggccgcga ctctagatca taatcagcca
     4021 taccacattt gtagaggttt tacttgcttt aaaaaacctc ccacacctcc ccctgaacct
     4081 gaaacataaa atgaatgcaa ttgttgttgt taacttgttt attgcagctt ataatggtta
     4141 caaataaagc aatagcatca caaatttcac aaataaagca tttttttcac tgcattctag
     4201 ttgtggtttg tccaaactca tcaatgtatc ttaaggcgta aattgtaagc gttaatattt
     4261 tgttaaaatt cgcgttaaat ttttgttaaa tcagctcatt ttttaaccaa taggccgaaa
     4321 tcggcaaaat cccttataaa tcaaaagaat agaccgagat agggttgagt gttgttccag
     4381 tttggaacaa gagtccacta ttaaagaacg tggactccaa cgtcaaaggg cgaaaaaccg
     4441 tctatcaggg cgatggccca ctacgtgaac catcacccta atcaagtttt ttggggtcga
     4501 ggtgccgtaa agcactaaat cggaacccta aagggagccc ccgatttaga gcttgacggg
     4561 gaaagccggc gaacgtggcg agaaaggaag ggaagaaagc gaaaggagcg ggcgctaggg
     4621 cgctggcaag tgtagcggtc acgctgcgcg taaccaccac acccgccgcg cttaatgcgc
     4681 cgctacaggg cgcgtcaggt ggcacttttc ggggaaatgt gcgcggaacc cctatttgtt
     4741 tatttttcta aatacattca aatatgtatc cgctcatgag acaataaccc tgataaatgc
     4801 ttcaataata ttgaaaaagg aagagtcctg aggcggaaag aaccagctgt ggaatgtgtg
     4861 tcagttaggg tgtggaaagt ccccaggctc cccagcaggc agaagtatgc aaagcatgca
     4921 tctcaattag tcagcaacca ggtgtggaaa gtccccaggc tccccagcag gcagaagtat
     4981 gcaaagcatg catctcaatt agtcagcaac catagtcccg cccctaactc cgcccatccc
     5041 gcccctaact ccgcccagtt ccgcccattc tccgccccat ggctgactaa ttttttttat
     5101 ttatgcagag gccgaggccg cctcggcctc tgagctattc cagaagtagt gaggaggctt
     5161 ttttggaggc ctaggctttt gcaaagatcg atcaagagac aggatgagga tcgtttcgca
     5221 tgattgaaca agatggattg cacgcaggtt ctccggccgc ttgggtggag aggctattcg
     5281 gctatgactg ggcacaacag acaatcggct gctctgatgc cgccgtgttc cggctgtcag
     5341 cgcaggggcg cccggttctt tttgtcaaga ccgacctgtc cggtgccctg aatgaactgc
     5401 aagacgaggc agcgcggcta tcgtggctgg ccacgacggg cgttccttgc gcagctgtgc
     5461 tcgacgttgt cactgaagcg ggaagggact ggctgctatt gggcgaagtg ccggggcagg
     5521 atctcctgtc atctcacctt gctcctgccg agaaagtatc catcatggct gatgcaatgc
     5581 ggcggctgca tacgcttgat ccggctacct gcccattcga ccaccaagcg aaacatcgca
     5641 tcgagcgagc acgtactcgg atggaagccg gtcttgtcga tcaggatgat ctggacgaag
     5701 agcatcaggg gctcgcgcca gccgaactgt tcgccaggct caaggcgagc atgcccgacg
     5761 gcgaggatct cgtcgtgacc catggcgatg cctgcttgcc gaatatcatg gtggaaaatg
     5821 gccgcttttc tggattcatc gactgtggcc ggctgggtgt ggcggaccgc tatcaggaca
     5881 tagcgttggc tacccgtgat attgctgaag agcttggcgg cgaatgggct gaccgcttcc
     5941 tcgtgcttta cggtatcgcc gctcccgatt cgcagcgcat cgccttctat cgccttcttg
     6001 acgagttctt ctgagcggga ctctggggtt cgaaatgacc gaccaagcga cgcccaacct
     6061 gccatcacga gatttcgatt ccaccgccgc cttctatgaa aggttgggct tcggaatcgt
     6121 tttccgggac gccggctgga tgatcctcca gcgcggggat ctcatgctgg agttcttcgc
     6181 ccaccctagg gggaggctaa ctgaaacacg gaaggagaca ataccggaag gaacccgcgc
     6241 tatgacggca ataaaaagac agaataaaac gcacggtgtt gggtcgtttg ttcataaacg
     6301 cggggttcgg tcccagggct ggcactctgt cgatacccca ccgagacccc attggggcca
     6361 atacgcccgc gtttcttcct tttccccacc ccacccccca agttcgggtg aaggcccagg
     6421 gctcgcagcc aacgtcgggg cggcaggccc tgccatagcc tcaggttact catatatact
     6481 ttagattgat ttaaaacttc atttttaatt taaaaggatc taggtgaaga tcctttttga
     6541 taatctcatg accaaaatcc cttaacgtga gttttcgttc cactgagcgt cagaccccgt
     6601 agaaaagatc aaaggatctt cttgagatcc tttttttctg cgcgtaatct gctgcttgca
     6661 aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg gatcaagagc taccaactct
     6721 ttttccgaag gtaactggct tcagcagagc gcagatacca aatactgttc ttctagtgta
     6781 gccgtagtta ggccaccact tcaagaactc tgtagcaccg cctacatacc tcgctctgct
     6841 aatcctgtta ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg ggttggactc
     6901 aagacgatag ttaccggata aggcgcagcg gtcgggctga acggggggtt cgtgcacaca
     6961 gcccagcttg gagcgaacga cctacaccga actgagatac ctacagcgtg agctatgaga
     7021 aagcgccacg cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg
     7081 aacaggagag cgcacgaggg agcttccagg gggaaacgcc tggtatcttt atagtcctgt
     7141 cgggtttcgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag gggggcggag
     7201 cctatggaaa aacgccagca acgcggcctt tttacggttc ctggcctttt gctggccttt
     7261 tgctcacatg ttctttcctg cgttatcccc tgattctgtg gataaccgta ttaccgccat
     7321 gcat
//
