LOCUS       sequence-496362-       11260 bp    DNA     circular SYN 07-JAN-2026
DEFINITION  Mammalian expression vector of talin1 N-terminal fragment fused with
            pdDronpa1.2.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11260)
  AUTHORS   Nishimura R, Barnett SFH, Jain K, Huang Z, Goult BT, Kanchanawong P
  TITLE     Optogenetic control of mechanotransduction based on light-induced 
            homodimerization of talin.
  JOURNAL   J Cell Sci. 2025 Nov 24:jcs.264110. doi: 10.1242/jcs.264110.
  PUBMED    41277405
REFERENCE   2  (bases 1 to 11260)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Jan 7, 2026 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Cell Sci.
            2025 Nov 24:jcs.264110. doi: 10.1242/jcs.264110."
COMMENT     Sequence Label: mApple-Talin1(1–1973)-pdDronpa1.2
FEATURES             Location/Qualifiers
     source          1..11260
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        61..364
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        365..568
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     519..539
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     regulatory      595..604
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             601..1308
                     /codon_start=1
                     /product="photostable monomeric derivative of DsRed (Shaner
                     et al., 2008)"
                     /label=mApple
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEENNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEA
                     FQTAKLKVTKGGPLPFAWDILSPQFMYGSKVYIKHPADIPDYFKLSFPEGFRWERVMNF
                     EDGGIIHVNQDSSLQDGVFIYKVKLRGTNFPSDGPVMQKKTMGWEASEERMYPEDGALK
                     SEIKKRLKLKDGGHYAAEVKTTYKAKKPVQLPGAYIVDIKLDIVSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     1014..1033
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     polyA_signal    8048..8169
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(8085..8104)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     8139..8158
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      complement(8176..8631)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(8313..8334)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     8525..8544
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     promoter        8658..8762
                     /gene="bla"
                     /label=AmpR promoter
     promoter        8764..9121
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     enhancer        8764..8955
                     /label=SV40 enhancer
                     /note="enhancer for the SV40 early promoter (Herr, 1993)"
     primer_bind     complement(8787..8807)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     rep_origin      8972..9107
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             9156..9950
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     CDS             9156..9950
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(9210..9229)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     9820..9839
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     complement(10138..10157)
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     polyA_signal    10182..10229
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     rep_origin      10558..11146
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     11047..11066
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata tggagttccg
       61 cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt
      121 gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca
      181 atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc
      241 aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta
      301 catgacctta tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac
      361 catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg actcacgggg
      421 atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg
      481 ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg gtaggcgtgt
      541 acggtgggag gtctatataa gcagagctgg tttagtgaac cgtcagatcc gctagccacc
      601 atggtgagca agggcgagga gaataacatg gccatcatca aggagttcat gcgcttcaag
      661 gtgcacatgg agggctccgt gaacggccac gagttcgaga tcgagggcga gggcgagggc
      721 cgcccctacg aggcctttca gaccgctaag ctgaaggtga ccaagggtgg ccccctgccc
      781 ttcgcctggg acatcctgtc ccctcagttc atgtacggct ccaaggtcta cattaagcac
      841 ccagccgaca tccccgacta cttcaagctg tccttccccg agggcttcag gtgggagcgc
      901 gtgatgaact tcgaggacgg cggcattatt cacgttaacc aggactcctc cctgcaggac
      961 ggcgtgttca tctacaaggt gaagctgcgc ggcaccaact tcccctccga cggccccgta
     1021 atgcagaaga agaccatggg ctgggaggcc tccgaggagc ggatgtaccc cgaggacggc
     1081 gccctgaaga gcgagatcaa gaagaggctg aagctgaagg acggcggcca ctacgccgcc
     1141 gaggtcaaga ccacctacaa ggccaagaag cccgtgcagc tgcccggcgc ctacatcgtc
     1201 gacatcaagt tggacatcgt gtcccacaac gaggactaca ccatcgtgga acagtacgaa
     1261 cgcgccgagg gccgccactc caccggcggc atggacgagc tgtacaaggt cgacctcgag
     1321 atggttgcgc tttcgctgaa gattagcatt gggaatgtgg tgaagacgat gcaatttgag
     1381 ccatctacca tggtgtacga cgcctgccgc atgattcgtg agcggatccc agaggccctg
     1441 gctggccctc ccaacgactt tgggctcttt ctgtcagatg atgaccccaa aaaaggcatc
     1501 tggctagagg ctgggaaagc tttggactac tacatgctcc gaaatgggga caccatggag
     1561 tacagaaaaa agcagagacc cctgaagata cggatgttag acggaacggt gaagactatc
     1621 atggtggatg actccaagac cgtcacagac atgctcatga ccatttgtgc ccgaattggt
     1681 atcaccaacc atgatgagta ttcactggtt cgagagctga tggaagaaaa gaaagatgag
     1741 gggacaggga ccctgagaaa agacaagacc ctactgcgag atgaaaagaa gatggagaag
     1801 ctaaagcaga agttgcacac agacgatgag ttgaactggc tggaccatgg gcggacactg
     1861 agggaacagg gagtggaaga gcatgagacg ttgctgctgc ggagaaagtt cttctactca
     1921 gaccagaatg tggattcccg agaccctgta cagctgaacc ttctctatgt gcaggcacga
     1981 gatgacatcc tgaatggctc ccatcctgtc tcctttgaca aggcctgtga atttgcaggc
     2041 ttccagtgcc agatccagtt tggacctcac aatgaacaga agcacaaggc tggcttcctt
     2101 gacctgaagg acttcctgcc caaggagtat gtgaagcaga agggagagcg taagatcttt
     2161 caggcacaca agaattgtgg gcagatgagt gaaattgagg ccaaggtacg ctacgtgaag
     2221 ctggcccgtt ccctcaagac ttacggtgtc tccttcttcc tagtcaagga aaagatgaag
     2281 gggaagaata aactggtgcc caggctgttg ggcatcacta aggagtgtgt gatgcgtgtg
     2341 gatgagaaga ccaaggaggt gattcaggaa tggagcctca ccaacatcaa acgctgggct
     2401 gcctctccca agagctttac tctggacttt ggagactatc aggatggcta ctactcagta
     2461 cagacgacag aaggcgagca gatcgcacag ctcattgctg gctacataga tatcatcctt
     2521 aagaagaaaa aaagcaagga ccattttggg ctggagggag atgaagagtc tactatgttg
     2581 gaggactcag tgtctcccaa aaagtcaaca gtccttcagc agcagtacaa ccgagtgggg
     2641 aaagtggagc acggctctgt ggctctgcca gccatcatgc gctctggagc ctctggtcct
     2701 gagaatttcc aggtggggag catgccacct gcccagcagc agatcaccag tggccagatg
     2761 caccgaggac acatgccacc tctgacttca gcccagcagg cgctcactgg aaccattaac
     2821 tccagcatgc aggctgtgca ggctgcccag gccactctgg atgactttga gactctaccc
     2881 cctcttggcc aggatgctgc ctccaaagct tggcgtaaga acaagatgga tgaatcaaag
     2941 catgagatcc actcccaggt agatgccatc acagctggca ctgcctctgt ggtaaacctg
     3001 acagcaggag atcctgcaga gacagactat acagcagtgg gctgtgcagt caccaccatc
     3061 tcttccaacc tgacggagat gtcccgtggg gtgaagctat tggctgccct gctggaggat
     3121 gaaggcggca atggccggcc ccttctgcaa gcagcaaagg gccttgcagg ggctgtgtcg
     3181 gaactgcttc gcagtgctca gcccgccagc gctgagcctc gtcagaacct gctgcaggca
     3241 gccgggaacg tgggccaggc cagtggggag ctgttgcagc aaattgggga aagtgacact
     3301 gacccccact tccaggatgt tctaatgcag ctagcaaacg cagtggcaag tgctgcagcc
     3361 gccctggtcc tcaaggccaa gagtgtggcc cagcgcacag aggattccgg gcttcagacc
     3421 caagtgattg ctgcagctac gcaatgtgct ctgtccactt cccagctggt ggcctgtacc
     3481 aaggtggtag cacctacaat cagctcacct gtgtgccaag aacagctagt cgaggctggg
     3541 cgactggtgg ccaaagctgt ggagggctgt gtgtctgcct cgcaagcagc tacagaagat
     3601 ggacagctgc ttcgaggggt aggagcagca gccacggctg tcacccaggc cctcaatgag
     3661 ctgctgcagc acgtgaaggc ccacgccacc ggagctgggc ctgccggtcg gtatgaccaa
     3721 gccactgata ccatcctcac cgtcactgaa aacatcttca gctccatggg tgatgctggg
     3781 gagatggtgc gccaggcccg catcctggcc caagccacct cagacctggt caatgctatc
     3841 aaggccgatg ctgaggggga gagtgatctg gagaactcta gaaagctcct gagtgctgcc
     3901 aagatcctcg ctgatgccac cgccaagatg gtggaggcgg ccaagggagc agccgcccac
     3961 cctgacagtg aggaacagca gcagcgactg cgtgaagcag ctgaggggct tcgcatggcc
     4021 accaatgcag ctgcgcagaa cgccatcaag aagaagttgg tgcagcgcct ggagcatgca
     4081 gccaagcaag ctgcagcctc tgcaacacag accattgctg cagcccaaca tgcagcctct
     4141 gcccccaagg cctctgcggg cccccagccc ctactggtgc agagctgtaa ggccgtggca
     4201 gagcagattc cgttgctggt gcagggtgtc cgaggaagtc aagctcagcc tgacagccct
     4261 agtgctcagc tcgccctcat tgctgccagc cagagcttcc tgcagccagg tgggaagatg
     4321 gtggcagcgg caaaagcctc agtgccaaca attcaggacc aggcttcagc gatgcagctg
     4381 agtcagtgcg caaagaacct aggcactgcc ctggccgagc tgcgcactgc tgctcagaag
     4441 gctcaggagg catgtggacc tttggagatg gattctgcac tgagtgttgt acaaaatcta
     4501 gagaaagacc tacaggaaat aaaagcagca gctcgagatg gcaagcttaa gcccttacct
     4561 ggggaaacaa tggagaagtg tacccaagat cttggcaaca gcaccaaagc ggtgagctct
     4621 gccatcgcca agctgctggg ggagattgcc cagggcaacg agaactatgc aggtattgca
     4681 gctcgggatg tagctggtgg actaaggtca ctagcccagg ctgcgcgggg tgtggctgcg
     4741 ctgacatcag atcctgcagt gcaggccatt gtgcttgaca cagccagcga cgttctggac
     4801 aaggccagca gcctcattga agaggcaaaa aaagcatctg gacacccagg ggacccagaa
     4861 agccagcaaa ggcttgctca ggtagctaaa gcagtgaccc aagccctgaa ccgctgtgtc
     4921 agctgcctgc ctggccaaag agacgtggat aatgccctac gagccgttgg agatgccagc
     4981 aagcgactcc tgagtgactt gcttcctcca agcacgggga catttcaaga agcacagagc
     5041 cgattgaatg aagctgcagc tgggttaaat caggccgcca cagaactggt gcaagcctcc
     5101 cgaggaactc ctcaggacct ggctcgggcc tccggccgat ttggacagga cttcagcacc
     5161 ttcctggaag ctggtgtgga gatggccgga caggccccga gccaggagga ccgagcccag
     5221 gtggtgtcca acctgaaggg catatccatg tcttcaagca aacttctcct tgccgctaag
     5281 gccttgtcca cagaccctgc ttctcccaac ctcaagagtc agctggctgc ggctgcccgg
     5341 gcagtgacgg acagcatcaa ccagctcatc accatgtgca cccagcaggc acctggccag
     5401 aaggagtgtg acaatgcact tcggcagcta gagacagtcc gagaactcct ggagaatcca
     5461 gtccagccca tcaacgacat gtcctacttc ggttgcttgg acagtgtcat ggagaactcc
     5521 aaggtcctag gtgaggccat gactggcatc tcccaaaatg ccaagaatgg aaatctgccg
     5581 gagtttggag atgccattgc cacagcctcc aaggctctct gtggcttcac ggaggcagcc
     5641 gcacaggcag catatctagt tggtgtctct gaccccaaca gccaagctgg acagcaagga
     5701 ctggtggaac ccacacagtt tgcccgtgca aaccaggcaa ttcagatggc ctgtcagagt
     5761 ctgggggagc ctggctgtac ccaggcccag gtgttatctg cagccactat tgtagccaaa
     5821 cacacatctg cattgtgtaa cagctgtcgc ctggcttccg ctaggactgc caatcccact
     5881 gccaagcgcc agtttgtaca gtcagccaag gaggtggcca acagtacagc caatcttgtc
     5941 aagaccatca aggcactaga tggggacttc acagaagaga accgtgccca gtgccgagca
     6001 gccacagccc ctctgctgga agctgtggat aacctgagtg cctttgcctc caaccctgag
     6061 ttctccagcg tccctgccca gatcagccct gagggcagag cagccatgga gcccattgta
     6121 atctctgcta agacaatgtt ggagagtgct ggagggctca tccagacagc tcgggcgtta
     6181 gcagtcaatc cccgagaccc cccacgttgg tctgtgctag ctggccactc tcggactgtc
     6241 tcagattcca tcaagaaact tattacaagc atgagggaca aagccccagg gcagctggag
     6301 tgtgagacag ccattgcggc tctgaacagc tgcttgcggg acctagatca ggcttcgctt
     6361 gccgctgtca gccagcagct tgctccccgt gaaggaatct ctcaagaggc tttgcacacc
     6421 cagatgctca ctgcagtgca agaaatttct catctcattg agccgctggc cagcgctgct
     6481 cgggctgaag cctcccagct gggacacaag gtgtcccaaa tggcccagta ctttgaacca
     6541 ctcaccctgg ctgcagtggg tgctgcgtct aagaccctga gccacccgca gcagatggca
     6601 cttctggacc agactaaaac gttggcagag tctgccttgc agttgctata tactgccaag
     6661 gaggctggtg gtaaccccaa gcaagcagca cacacccagg aagccctgga ggaggctgtg
     6721 cagatgatga cagaggccgt agaagacctg acgacgaccc tcaacgaagc agccagtgct
     6781 gcaggagtcg ttggcggcat ggtggactct atcactcagg ccatcaacca gctagatgaa
     6841 ggacctatgg gtgacccaga aggctcattc gtagattacc agacaaccat ggtgaggaca
     6901 gccaaggcca ttgctgtcac tgttcaggag atggtaacca agtcaaacac cagccctgaa
     6961 gagctaggcc ctcttgccaa ccagctgacc agtgactatg gccgactggc ctcacaagcc
     7021 aagcctgcag ctgtggctgc tgaaaatgaa gagataggcg ctcacatcaa acaccgagta
     7081 caggagctgg gccatggctg ctctgctctg gtcaccaagg caggtgcctt gcagtgtagc
     7141 cccagtgatg tctacaccaa gaaggagctc atagagtgtg cccgcagagt gtcagagaag
     7201 gtctcccatg ttctggctgc actccaggct gggaatcgtg aattcaccgg ttctggatcc
     7261 tccgtgatta aaccagacat gaagatcaag ctgcgtatgg aaggcgctgt aaatggacac
     7321 ccgttcgcga ttgaaggagt tggccttggg aagcctttcg agggaaaaca gagtattgac
     7381 cttaaagtca aagaaggcgg acctctgcct ttcgcctatg acatcttgac aactgctttc
     7441 tgttacggca acagggtatt cgccaaatac ccagaaaata tagtagacta tttcaagcag
     7501 tcgtttcctg agggctactc ttgggaacga agcatgaatt acgaagacgg gggcatttgt
     7561 aacgcgacaa acgacataac cctggatggt gactgttata tctatgaaat tcgatttaga
     7621 ggtacgaact ttcctgccaa tggtccagtt atgcagaaga ggactgtgaa atgggagcca
     7681 tccactgaga acttgtatgt gcgtgatgga gtgctgaagg gtgatgttat catggctctg
     7741 tcgcttgaag gaggtggcca ttaccgatgt gacttcaaaa ctacttataa agctaagaag
     7801 gttgtccagt tgccagacta tcactttgtg gaccaccaca ttgagattaa aagccacgac
     7861 aaagattaca gtaatgttaa tctgcatgag cacgccgaag cgcattctga gctgccgagg
     7921 cagtaagcgg ccgcgactct agatcataat cagccatacc acatttgtag aggttttact
     7981 tgctttaaaa aacctcccac acctccccct gaacctgaaa cataaaatga atgcaattgt
     8041 tgttgttaac ttgtttattg cagcttataa tggttacaaa taaagcaata gcatcacaaa
     8101 tttcacaaat aaagcatttt tttcactgca ttctagttgt ggtttgtcca aactcatcaa
     8161 tgtatcttaa ggcgtaaatt gtaagcgtta atattttgtt aaaattcgcg ttaaattttt
     8221 gttaaatcag ctcatttttt aaccaatagg ccgaaatcgg caaaatccct tataaatcaa
     8281 aagaatagac cgagataggg ttgagtgttg ttccagtttg gaacaagagt ccactattaa
     8341 agaacgtgga ctccaacgtc aaagggcgaa aaaccgtcta tcagggcgat ggcccactac
     8401 gtgaaccatc accctaatca agttttttgg ggtcgaggtg ccgtaaagca ctaaatcgga
     8461 accctaaagg gagcccccga tttagagctt gacggggaaa gccggcgaac gtggcgagaa
     8521 aggaagggaa gaaagcgaaa ggagcgggcg ctagggcgct ggcaagtgta gcggtcacgc
     8581 tgcgcgtaac caccacaccc gccgcgctta atgcgccgct acagggcgcg tcaggtggca
     8641 cttttcgggg aaatgtgcgc ggaaccccta tttgtttatt tttctaaata cattcaaata
     8701 tgtatccgct catgagacaa taaccctgat aaatgcttca ataatattga aaaaggaaga
     8761 gtcctgaggc ggaaagaacc agctgtggaa tgtgtgtcag ttagggtgtg gaaagtcccc
     8821 aggctcccca gcaggcagaa gtatgcaaag catgcatctc aattagtcag caaccaggtg
     8881 tggaaagtcc ccaggctccc cagcaggcag aagtatgcaa agcatgcatc tcaattagtc
     8941 agcaaccata gtcccgcccc taactccgcc catcccgccc ctaactccgc ccagttccgc
     9001 ccattctccg ccccatggct gactaatttt ttttatttat gcagaggccg aggccgcctc
     9061 ggcctctgag ctattccaga agtagtgagg aggctttttt ggaggcctag gcttttgcaa
     9121 agatcgatca agagacagga tgaggatcgt ttcgcatgat tgaacaagat ggattgcacg
     9181 caggttctcc ggccgcttgg gtggagaggc tattcggcta tgactgggca caacagacaa
     9241 tcggctgctc tgatgccgcc gtgttccggc tgtcagcgca ggggcgcccg gttctttttg
     9301 tcaagaccga cctgtccggt gccctgaatg aactgcaaga cgaggcagcg cggctatcgt
     9361 ggctggccac gacgggcgtt ccttgcgcag ctgtgctcga cgttgtcact gaagcgggaa
     9421 gggactggct gctattgggc gaagtgccgg ggcaggatct cctgtcatct caccttgctc
     9481 ctgccgagaa agtatccatc atggctgatg caatgcggcg gctgcatacg cttgatccgg
     9541 ctacctgccc attcgaccac caagcgaaac atcgcatcga gcgagcacgt actcggatgg
     9601 aagccggtct tgtcgatcag gatgatctgg acgaagagca tcaggggctc gcgccagccg
     9661 aactgttcgc caggctcaag gcgagcatgc ccgacggcga ggatctcgtc gtgacccatg
     9721 gcgatgcctg cttgccgaat atcatggtgg aaaatggccg cttttctgga ttcatcgact
     9781 gtggccggct gggtgtggcg gaccgctatc aggacatagc gttggctacc cgtgatattg
     9841 ctgaagagct tggcggcgaa tgggctgacc gcttcctcgt gctttacggt atcgccgctc
     9901 ccgattcgca gcgcatcgcc ttctatcgcc ttcttgacga gttcttctga gcgggactct
     9961 ggggttcgaa atgaccgacc aagcgacgcc caacctgcca tcacgagatt tcgattccac
    10021 cgccgccttc tatgaaaggt tgggcttcgg aatcgttttc cgggacgccg gctggatgat
    10081 cctccagcgc ggggatctca tgctggagtt cttcgcccac cctaggggga ggctaactga
    10141 aacacggaag gagacaatac cggaaggaac ccgcgctatg acggcaataa aaagacagaa
    10201 taaaacgcac ggtgttgggt cgtttgttca taaacgcggg gttcggtccc agggctggca
    10261 ctctgtcgat accccaccga gaccccattg gggccaatac gcccgcgttt cttccttttc
    10321 cccaccccac cccccaagtt cgggtgaagg cccagggctc gcagccaacg tcggggcggc
    10381 aggccctgcc atagcctcag gttactcata tatactttag attgatttaa aacttcattt
    10441 ttaatttaaa aggatctagg tgaagatcct ttttgataat ctcatgacca aaatccctta
    10501 acgtgagttt tcgttccact gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg
    10561 agatcctttt tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac cgctaccagc
    10621 ggtggtttgt ttgccggatc aagagctacc aactcttttt ccgaaggtaa ctggcttcag
    10681 cagagcgcag ataccaaata ctgttcttct agtgtagccg tagttaggcc accacttcaa
    10741 gaactctgta gcaccgccta catacctcgc tctgctaatc ctgttaccag tggctgctgc
    10801 cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac cggataaggc
    10861 gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc agcttggagc gaacgaccta
    10921 caccgaactg agatacctac agcgtgagct atgagaaagc gccacgcttc ccgaagggag
    10981 aaaggcggac aggtatccgg taagcggcag ggtcggaaca ggagagcgca cgagggagct
    11041 tccaggggga aacgcctggt atctttatag tcctgtcggg tttcgccacc tctgacttga
    11101 gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc
    11161 ggccttttta cggttcctgg ccttttgctg gccttttgct cacatgttct ttcctgcgtt
    11221 atcccctgat tctgtggata accgtattac cgccatgcat
//
