LOCUS       sequence-496363-       13144 bp    DNA     circular SYN 07-JAN-2026
DEFINITION  Mammalian expression vector of opto-dimerizable talin1.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 13144)
  AUTHORS   Nishimura R, Barnett SFH, Jain K, Huang Z, Goult BT, Kanchanawong P
  TITLE     Optogenetic control of mechanotransduction based on light-induced 
            homodimerization of talin.
  JOURNAL   J Cell Sci. 2025 Nov 24:jcs.264110. doi: 10.1242/jcs.264110.
  PUBMED    41277405
REFERENCE   2  (bases 1 to 13144)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Jan 7, 2026 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Cell Sci.
            2025 Nov 24:jcs.264110. doi: 10.1242/jcs.264110."
COMMENT     Sequence Label: mIFP-Talin1(1–2493)-pdDronpa1.2
FEATURES             Location/Qualifiers
     source          1..13144
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        61..364
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        365..568
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     519..539
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     regulatory      595..604
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             604..627
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             643..1602
                     /codon_start=1
                     /product="naturally monomeric infrared fluorescent protein 
                     derived from a truncated bacteriophytochrome (Yu et al., 
                     2015)"
                     /label=mIFP
                     /translation="MSVPLTTSAFGHAFLANCEREQIHLAGSIQPHGILLAVKEPDNVV
                     IQASINAAEFLNTNSVVGRPLRDLGGDLPLQILPHLNGPLHLAPMTLRCTVGSPPRRVD
                     CTIHRPSNGGLIVELEPATKTTNIAPALDGAFHRITSSSSLMGLCDETATIIREITGYD
                     RVMVVRFDEEGNGEILSERRRADLEAFLGNRYPASTIPQIARRLYEHNRVRLLVDVNYT
                     PVPLQPRISPLNGRDLDMSLSCLRSMSPIHQKYMQDMGVGATLVCSLMVSGRLWGLIAC
                     HHYEPRFVPFHIRAAGEALAETCAIRIATLESFAQSQSK"
     polyA_signal    9932..10053
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(9969..9988)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     10023..10042
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      complement(10060..10515)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(10197..10218)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     10409..10428
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     promoter        10542..10646
                     /gene="bla"
                     /label=AmpR promoter
     promoter        10648..11005
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     enhancer        10648..10839
                     /label=SV40 enhancer
                     /note="enhancer for the SV40 early promoter (Herr, 1993)"
     primer_bind     complement(10671..10691)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     rep_origin      10856..10991
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             11040..11834
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     CDS             11040..11834
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(11094..11113)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     11704..11723
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     complement(12022..12041)
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     polyA_signal    12066..12113
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     rep_origin      12442..13030
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     12931..12950
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata tggagttccg
       61 cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt
      121 gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca
      181 atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc
      241 aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta
      301 catgacctta tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac
      361 catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg actcacgggg
      421 atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg
      481 ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg gtaggcgtgt
      541 acggtgggag gtctatataa gcagagctgg tttagtgaac cgtcagatcc gctagccacc
      601 atggactaca aagacgatga cgacaagggg tccgggtccg ggatgtcggt accgctgact
      661 acctcagcat tcggccacgc gtttctggct aactgtgaac gcgagcagat ccacctggcg
      721 ggctccattc agccgcacgg tatcctgctg gctgtgaaag agccggacaa cgtggtgatc
      781 caggcttcta ttaacgctgc ggagttcctg aacaccaact ctgttgttgg ccgtccgctg
      841 cgtgacctgg gcggcgatct gcctttgcag atcctgccgc acctgaacgg cccgctgcac
      901 ctggctccga tgaccctgcg ttgtaccgtg ggttctccgc cgcgtcgtgt ggactgtacc
      961 attcatcgtc cgtctaacgg cggcctgatc gtagaactgg aaccagcaac caagaccact
     1021 aacattgcgc cggctctgga cggtgcgttt catcgtatca cttcttcatc ctccctgatg
     1081 ggcctgtgtg acgaaaccgc gactattatc cgtgagatta ctggctacga ccgtgtgatg
     1141 gtagtacgtt tcgatgaaga gggtaatggc gaaattctgt ccgaacgtcg tcgtgcggac
     1201 ctggaagcgt tcctgggtaa ccgctacccg gcgtctacta ttccgcagat cgctcgtcgc
     1261 ctgtacgaac ataaccgtgt tcgcctgctg gtagatgtga actatactcc ggttccgcta
     1321 cagccgcgca tcagcccgct gaacggtcgt gatctggata tgtccctgtc ttgcctgcgc
     1381 tctatgtccc cgatccacca gaaatacatg caggacatgg gcgttggcgc gaccctggtt
     1441 tgctctctga tggtgtctgg tcgtctgtgg ggtctgatcg cttgccacca ctacgaaccg
     1501 cgcttcgttc cgttccacat tcgcgctgct ggcgaagcgc tggcggaaac ttgtgcgatc
     1561 cgcatcgcga cgctggagag ctttgcacag tctcagtcca aaggcgctgg gtccgggtcc
     1621 gggcaagggt ccggcgcagg ctccgcgtcc atggttgcgc tttcgctgaa gattagcatt
     1681 gggaatgtgg tgaagacgat gcaatttgag ccatctacca tggtgtacga cgcctgccgc
     1741 atgattcgtg agcggatccc agaggccctg gctggccctc ccaacgactt tgggctcttt
     1801 ctgtcagatg atgaccccaa aaaaggcatc tggctagagg ctgggaaagc tttggactac
     1861 tacatgctcc gaaatgggga caccatggag tacagaaaaa agcagagacc cctgaagata
     1921 cggatgttag acggaacggt gaagactatc atggtggatg actccaagac cgtcacagac
     1981 atgctcatga ccatttgtgc ccgaattggt atcaccaacc atgatgagta ttcactggtt
     2041 cgagagctga tggaagaaaa gaaagatgag gggacaggga ccctgagaaa agacaagacc
     2101 ctactgcgag atgaaaagaa gatggagaag ctaaagcaga agttgcacac agacgatgag
     2161 ttgaactggc tggaccatgg gcggacactg agggaacagg gagtggaaga gcatgagacg
     2221 ttgctgctgc ggagaaagtt cttctactca gaccagaatg tggattcccg agaccctgta
     2281 cagctgaacc ttctctatgt gcaggcacga gatgacatcc tgaatggctc ccatcctgtc
     2341 tcctttgaca aggcctgtga atttgcaggc ttccagtgcc agatccagtt tggacctcac
     2401 aatgaacaga agcacaaggc tggcttcctt gacctgaagg acttcctgcc caaggagtat
     2461 gtgaagcaga agggagagcg taagatcttt caggcacaca agaattgtgg gcagatgagt
     2521 gaaattgagg ccaaggtacg ctacgtgaag ctggcccgtt ccctcaagac ttacggtgtc
     2581 tccttcttcc tagtcaagga aaagatgaag gggaagaata aactggtgcc caggctgttg
     2641 ggcatcacta aggagtgtgt gatgcgtgtg gatgagaaga ccaaggaggt gattcaggaa
     2701 tggagcctca ccaacatcaa acgctgggct gcctctccca agagctttac tctggacttt
     2761 ggagactatc aggatggcta ctactcagta cagacgacag aaggcgagca gatcgcacag
     2821 ctcattgctg gctacataga tatcatcctt aagaagaaaa aaagcaagga ccattttggg
     2881 ctggagggag atgaagagtc tactatgttg gaggactcag tgtctcccaa aaagtcaaca
     2941 gtccttcagc agcagtacaa ccgagtgggg aaagtggagc acggctctgt ggctctgcca
     3001 gccatcatgc gctctggagc ctctggtcct gagaatttcc aggtggggag catgccacct
     3061 gcccagcagc agatcaccag tggccagatg caccgaggac acatgccacc tctgacttca
     3121 gcccagcagg cgctcactgg aaccattaac tccagcatgc aggctgtgca ggctgcccag
     3181 gccactctgg atgactttga gactctaccc cctcttggcc aggatgctgc ctccaaagct
     3241 tggcgtaaga acaagatgga tgaatcaaag catgagatcc actcccaggt agatgccatc
     3301 acagctggca ctgcctctgt ggtaaacctg acagcaggag atcctgcaga gacagactat
     3361 acagcagtgg gctgtgcagt caccaccatc tcttccaacc tgacggagat gtcccgtggg
     3421 gtgaagctat tggctgccct gctggaggat gaaggcggca atggccggcc ccttctgcaa
     3481 gcagcaaagg gccttgcagg ggctgtgtcg gaactgcttc gcagtgctca gcccgccagc
     3541 gctgagcctc gtcagaacct gctgcaggca gccgggaacg tgggccaggc cagtggggag
     3601 ctgttgcagc aaattgggga aagtgacact gacccccact tccaggatgt tctaatgcag
     3661 ctagcaaacg cagtggcaag tgctgcagcc gccctggtcc tcaaggccaa gagtgtggcc
     3721 cagcgcacag aggattccgg gcttcagacc caagtgattg ctgcagctac gcaatgtgct
     3781 ctgtccactt cccagctggt ggcctgtacc aaggtggtag cacctacaat cagctcacct
     3841 gtgtgccaag aacagctagt cgaggctggg cgactggtgg ccaaagctgt ggagggctgt
     3901 gtgtctgcct cgcaagcagc tacagaagat ggacagctgc ttcgaggggt aggagcagca
     3961 gccacggctg tcacccaggc cctcaatgag ctgctgcagc acgtgaaggc ccacgccacc
     4021 ggagctgggc ctgccggtcg gtatgaccaa gccactgata ccatcctcac cgtcactgaa
     4081 aacatcttca gctccatggg tgatgctggg gagatggtgc gccaggcccg catcctggcc
     4141 caagccacct cagacctggt caatgctatc aaggccgatg ctgaggggga gagtgatctg
     4201 gagaactcta gaaagctcct gagtgctgcc aagatcctcg ctgatgccac cgccaagatg
     4261 gtggaggcgg ccaagggagc agccgcccac cctgacagtg aggaacagca gcagcgactg
     4321 cgtgaagcag ctgaggggct tcgcatggcc accaatgcag ctgcgcagaa cgccatcaag
     4381 aagaagttgg tgcagcgcct ggagcatgca gccaagcaag ctgcagcctc tgcaacacag
     4441 accattgctg cagcccaaca tgcagcctct gcccccaagg cctctgcggg cccccagccc
     4501 ctactggtgc agagctgtaa ggccgtggca gagcagattc cgttgctggt gcagggtgtc
     4561 cgaggaagtc aagctcagcc tgacagccct agtgctcagc tcgccctcat tgctgccagc
     4621 cagagcttcc tgcagccagg tgggaagatg gtggcagcgg caaaagcctc agtgccaaca
     4681 attcaggacc aggcttcagc gatgcagctg agtcagtgcg caaagaacct aggcactgcc
     4741 ctggccgagc tgcgcactgc tgctcagaag gctcaggagg catgtggacc tttggagatg
     4801 gattctgcac tgagtgttgt acaaaatcta gagaaagacc tacaggaaat aaaagcagca
     4861 gctcgagatg gcaagcttaa gcccttacct ggggaaacaa tggagaagtg tacccaagat
     4921 cttggcaaca gcaccaaagc ggtgagctct gccatcgcca agctgctggg ggagattgcc
     4981 cagggcaacg agaactatgc aggtattgca gctcgggatg tagctggtgg actaaggtca
     5041 ctagcccagg ctgcgcgggg tgtggctgcg ctgacatcag atcctgcagt gcaggccatt
     5101 gtgcttgaca cagccagcga cgttctggac aaggccagca gcctcattga agaggcaaaa
     5161 aaagcatctg gacacccagg ggacccagaa agccagcaaa ggcttgctca ggtagctaaa
     5221 gcagtgaccc aagccctgaa ccgctgtgtc agctgcctgc ctggccaaag agacgtggat
     5281 aatgccctac gagccgttgg agatgccagc aagcgactcc tgagtgactt gcttcctcca
     5341 agcacgggga catttcaaga agcacagagc cgattgaatg aagctgcagc tgggttaaat
     5401 caggccgcca cagaactggt gcaagcctcc cgaggaactc ctcaggacct ggctcgggcc
     5461 tccggccgat ttggacagga cttcagcacc ttcctggaag ctggtgtgga gatggccgga
     5521 caggccccga gccaggagga ccgagcccag gtggtgtcca acctgaaggg catatccatg
     5581 tcttcaagca aacttctcct tgccgctaag gccttgtcca cagaccctgc ttctcccaac
     5641 ctcaagagtc agctggctgc ggctgcccgg gcagtgacgg acagcatcaa ccagctcatc
     5701 accatgtgca cccagcaggc acctggccag aaggagtgtg acaatgcact tcggcagcta
     5761 gagacagtcc gagaactcct ggagaatcca gtccagccca tcaacgacat gtcctacttc
     5821 ggttgcttgg acagtgtcat ggagaactcc aaggtcctag gtgaggccat gactggcatc
     5881 tcccaaaatg ccaagaatgg aaatctgccg gagtttggag atgccattgc cacagcctcc
     5941 aaggctctct gtggcttcac ggaggcagcc gcacaggcag catatctagt tggtgtctct
     6001 gaccccaaca gccaagctgg acagcaagga ctggtggaac ccacacagtt tgcccgtgca
     6061 aaccaggcaa ttcagatggc ctgtcagagt ctgggggagc ctggctgtac ccaggcccag
     6121 gtgttatctg cagccactat tgtagccaaa cacacatctg cattgtgtaa cagctgtcgc
     6181 ctggcttccg ctaggactgc caatcccact gccaagcgcc agtttgtaca gtcagccaag
     6241 gaggtggcca acagtacagc caatcttgtc aagaccatca aggcactaga tggggacttc
     6301 acagaagaga accgtgccca gtgccgagca gccacagccc ctctgctgga agctgtggat
     6361 aacctgagtg cctttgcctc caaccctgag ttctccagcg tccctgccca gatcagccct
     6421 gagggcagag cagccatgga gcccattgta atctctgcta agacaatgtt ggagagtgct
     6481 ggagggctca tccagacagc tcgggcgtta gcagtcaatc cccgagaccc cccacgttgg
     6541 tctgtgctag ctggccactc tcggactgtc tcagattcca tcaagaaact tattacaagc
     6601 atgagggaca aagccccagg gcagctggag tgtgagacag ccattgcggc tctgaacagc
     6661 tgcttgcggg acctagatca ggcttcgctt gccgctgtca gccagcagct tgctccccgt
     6721 gaaggaatct ctcaagaggc tttgcacacc cagatgctca ctgcagtgca agaaatttct
     6781 catctcattg agccgctggc cagcgctgct cgggctgaag cctcccagct gggacacaag
     6841 gtgtcccaaa tggcccagta ctttgaacca ctcaccctgg ctgcagtggg tgctgcgtct
     6901 aagaccctga gccacccgca gcagatggca cttctggacc agactaaaac gttggcagag
     6961 tctgccttgc agttgctata tactgccaag gaggctggtg gtaaccccaa gcaagcagca
     7021 cacacccagg aagccctgga ggaggctgtg cagatgatga cagaggccgt agaagacctg
     7081 acgacgaccc tcaacgaagc agccagtgct gcaggagtcg ttggcggcat ggtggactct
     7141 atcactcagg ccatcaacca gctagatgaa ggacctatgg gtgacccaga aggctcattc
     7201 gtagattacc agacaaccat ggtgaggaca gccaaggcca ttgctgtcac tgttcaggag
     7261 atggtaacca agtcaaacac cagccctgaa gagctaggcc ctcttgccaa ccagctgacc
     7321 agtgactatg gccgactggc ctcacaagcc aagcctgcag ctgtggctgc tgaaaatgaa
     7381 gagataggcg ctcacatcaa acaccgagta caggagctgg gccatggctg ctctgctctg
     7441 gtcaccaagg caggtgcctt gcagtgtagc cccagtgatg tctacaccaa gaaggagctc
     7501 atagagtgtg cccgcagagt gtcagagaag gtctcccatg ttctggctgc actccaggct
     7561 gggaatcgtg gtacccaggc ctgcattaca gcagccagtg ctgtgtctgg tatcattgct
     7621 gacctcgaca ccaccatcat gttcgctact gctggcacac ttaaccgtga gggtgctgaa
     7681 acttttgctg accaccggga gggtatctta aagacagcaa aggttcttgt ggaggacacc
     7741 aaggtcctag tgcagaatgc agctgggagc caggagaagt tggcacaagc cgcccagtcc
     7801 tccgtggcca ccattacccg cctcgctgat gtggtcaagc tcggtgcagc cagcctagga
     7861 gccgaagacc ctgaaactca ggtggtgctg atcaatgcag taaaggacgt agccaaggcc
     7921 ctgggtgacc tcatcagcgc tacgaaggct gcagcgggca aagttgggga tgaccctgca
     7981 gtgtggcagc tcaagaactc tgccaaggtg atggtgacca atgtgacatc attgctcaag
     8041 acagtgaagg ctgtggaaga tgaggccacc aaaggcacac gggccctaga ggcaaccaca
     8101 gagcacatac gtcaggaact ggcggtcttc tgttccccag agccacctgc caagacctct
     8161 acccctgaag acttcatccg aatgaccaag ggtattacta tggcaacagc caaagccgtt
     8221 gctgctggca attcctgtcg acaggaagat gtcattgcca cagccaatct gagccgacgg
     8281 gctattgcgg acatgcttcg ggcttgcaag gaagcagctt tccacccaga agtggcgcct
     8341 gatgtacggc tccgagccct gcactatggc cgggagtgtg ccaatggtta cctggaactg
     8401 ctggaccacg tgctgctgac ccttcagaag ccaaacccag acctgaagca gcagctaact
     8461 ggacactcaa agcgggttgc tggctctgtg actgagctca tccaggctgc tgaagccatg
     8521 aaaggaacag agtgggtgga cccagaggac cctactgtca ttgctgagaa tgagctcttg
     8581 ggagccgcag ccgccatcga ggctgcagcc aagaagctgg agcagctgaa gcccagggcc
     8641 aaacccaagg aggcggatga gtccttgaac tttgaggaac aaatcctaga agctgccaag
     8701 tccatcgctg cagccaccag tgcactggta aaggctgcgt cagcagccca gagggagctg
     8761 gtggctcaag gaaaggtggg cgccattcca gccaatgcac tggatgatgg gcagtggtcg
     8821 cagggcctca tttccgctgc ccgtatggtg gctgcggcca ccaacaatct gtgtgaagca
     8881 gctaacgcag ctgtccaggg ccatgctagc caggagaaac tcatttcctc agccaagcaa
     8941 gtagctgcct ccacagctca gctcctggta gcttgcaagg tcaaagcaga tcaggactct
     9001 gaggcaatga aacggctcca ggctgctggc aatgcagtga agagggcctc agataacctg
     9061 gtaaaggcgg cccagaaagc tgcagccttc gaagaccagg agaatgagac ggtggtggtg
     9121 aaggagaaag ggtccgggtc cgggtccgtg attaaaccag acatgaagat caagctgcgt
     9181 atggaaggcg ctgtaaatgg acacccgttc gcgattgaag gagttggcct tgggaagcct
     9241 ttcgagggaa aacagagtat tgaccttaaa gtcaaagaag gcggacctct gcctttcgcc
     9301 tatgacatct tgacaactgc tttctgttac ggcaacaggg tattcgccaa atacccagaa
     9361 aatatagtag actatttcaa gcagtcgttt cctgagggct actcttggga acgaagcatg
     9421 aattacgaag acgggggcat ttgtaacgcg acaaacgaca taaccctgga tggtgactgt
     9481 tatatctatg aaattcgatt tagaggtacg aactttcctg ccaatggtcc agttatgcag
     9541 aagaggactg tgaaatggga gccatccact gagaacttgt atgtgcgtga tggagtgctg
     9601 aagggtgatg ttatcatggc tctgtcgctt gaaggaggtg gccattaccg atgtgacttc
     9661 aaaactactt ataaagctaa gaaggttgtc cagttgccag actatcactt tgtggaccac
     9721 cacattgaga ttaaaagcca cgacaaagat tacagtaatg ttaatctgca tgagcacgcc
     9781 gaagcgcatt ctgagctgcc gaggcagtaa gcggccgcga ctctagatca taatcagcca
     9841 taccacattt gtagaggttt tacttgcttt aaaaaacctc ccacacctcc ccctgaacct
     9901 gaaacataaa atgaatgcaa ttgttgttgt taacttgttt attgcagctt ataatggtta
     9961 caaataaagc aatagcatca caaatttcac aaataaagca tttttttcac tgcattctag
    10021 ttgtggtttg tccaaactca tcaatgtatc ttaaggcgta aattgtaagc gttaatattt
    10081 tgttaaaatt cgcgttaaat ttttgttaaa tcagctcatt ttttaaccaa taggccgaaa
    10141 tcggcaaaat cccttataaa tcaaaagaat agaccgagat agggttgagt gttgttccag
    10201 tttggaacaa gagtccacta ttaaagaacg tggactccaa cgtcaaaggg cgaaaaaccg
    10261 tctatcaggg cgatggccca ctacgtgaac catcacccta atcaagtttt ttggggtcga
    10321 ggtgccgtaa agcactaaat cggaacccta aagggagccc ccgatttaga gcttgacggg
    10381 gaaagccggc gaacgtggcg agaaaggaag ggaagaaagc gaaaggagcg ggcgctaggg
    10441 cgctggcaag tgtagcggtc acgctgcgcg taaccaccac acccgccgcg cttaatgcgc
    10501 cgctacaggg cgcgtcaggt ggcacttttc ggggaaatgt gcgcggaacc cctatttgtt
    10561 tatttttcta aatacattca aatatgtatc cgctcatgag acaataaccc tgataaatgc
    10621 ttcaataata ttgaaaaagg aagagtcctg aggcggaaag aaccagctgt ggaatgtgtg
    10681 tcagttaggg tgtggaaagt ccccaggctc cccagcaggc agaagtatgc aaagcatgca
    10741 tctcaattag tcagcaacca ggtgtggaaa gtccccaggc tccccagcag gcagaagtat
    10801 gcaaagcatg catctcaatt agtcagcaac catagtcccg cccctaactc cgcccatccc
    10861 gcccctaact ccgcccagtt ccgcccattc tccgccccat ggctgactaa ttttttttat
    10921 ttatgcagag gccgaggccg cctcggcctc tgagctattc cagaagtagt gaggaggctt
    10981 ttttggaggc ctaggctttt gcaaagatcg atcaagagac aggatgagga tcgtttcgca
    11041 tgattgaaca agatggattg cacgcaggtt ctccggccgc ttgggtggag aggctattcg
    11101 gctatgactg ggcacaacag acaatcggct gctctgatgc cgccgtgttc cggctgtcag
    11161 cgcaggggcg cccggttctt tttgtcaaga ccgacctgtc cggtgccctg aatgaactgc
    11221 aagacgaggc agcgcggcta tcgtggctgg ccacgacggg cgttccttgc gcagctgtgc
    11281 tcgacgttgt cactgaagcg ggaagggact ggctgctatt gggcgaagtg ccggggcagg
    11341 atctcctgtc atctcacctt gctcctgccg agaaagtatc catcatggct gatgcaatgc
    11401 ggcggctgca tacgcttgat ccggctacct gcccattcga ccaccaagcg aaacatcgca
    11461 tcgagcgagc acgtactcgg atggaagccg gtcttgtcga tcaggatgat ctggacgaag
    11521 agcatcaggg gctcgcgcca gccgaactgt tcgccaggct caaggcgagc atgcccgacg
    11581 gcgaggatct cgtcgtgacc catggcgatg cctgcttgcc gaatatcatg gtggaaaatg
    11641 gccgcttttc tggattcatc gactgtggcc ggctgggtgt ggcggaccgc tatcaggaca
    11701 tagcgttggc tacccgtgat attgctgaag agcttggcgg cgaatgggct gaccgcttcc
    11761 tcgtgcttta cggtatcgcc gctcccgatt cgcagcgcat cgccttctat cgccttcttg
    11821 acgagttctt ctgagcggga ctctggggtt cgaaatgacc gaccaagcga cgcccaacct
    11881 gccatcacga gatttcgatt ccaccgccgc cttctatgaa aggttgggct tcggaatcgt
    11941 tttccgggac gccggctgga tgatcctcca gcgcggggat ctcatgctgg agttcttcgc
    12001 ccaccctagg gggaggctaa ctgaaacacg gaaggagaca ataccggaag gaacccgcgc
    12061 tatgacggca ataaaaagac agaataaaac gcacggtgtt gggtcgtttg ttcataaacg
    12121 cggggttcgg tcccagggct ggcactctgt cgatacccca ccgagacccc attggggcca
    12181 atacgcccgc gtttcttcct tttccccacc ccacccccca agttcgggtg aaggcccagg
    12241 gctcgcagcc aacgtcgggg cggcaggccc tgccatagcc tcaggttact catatatact
    12301 ttagattgat ttaaaacttc atttttaatt taaaaggatc taggtgaaga tcctttttga
    12361 taatctcatg accaaaatcc cttaacgtga gttttcgttc cactgagcgt cagaccccgt
    12421 agaaaagatc aaaggatctt cttgagatcc tttttttctg cgcgtaatct gctgcttgca
    12481 aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg gatcaagagc taccaactct
    12541 ttttccgaag gtaactggct tcagcagagc gcagatacca aatactgttc ttctagtgta
    12601 gccgtagtta ggccaccact tcaagaactc tgtagcaccg cctacatacc tcgctctgct
    12661 aatcctgtta ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg ggttggactc
    12721 aagacgatag ttaccggata aggcgcagcg gtcgggctga acggggggtt cgtgcacaca
    12781 gcccagcttg gagcgaacga cctacaccga actgagatac ctacagcgtg agctatgaga
    12841 aagcgccacg cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg
    12901 aacaggagag cgcacgaggg agcttccagg gggaaacgcc tggtatcttt atagtcctgt
    12961 cgggtttcgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag gggggcggag
    13021 cctatggaaa aacgccagca acgcggcctt tttacggttc ctggcctttt gctggccttt
    13081 tgctcacatg ttctttcctg cgttatcccc tgattctgtg gataaccgta ttaccgccat
    13141 gcat
//
