LOCUS       sequence-496364-       12466 bp    DNA     circular SYN 07-JAN-2026
DEFINITION  Mammalian expression vector of truncated talin1 lacks C-terminal 
            dimerization domain (Delta-DD).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12466)
  AUTHORS   Nishimura R, Barnett SFH, Jain K, Huang Z, Goult BT, Kanchanawong P
  TITLE     Optogenetic control of mechanotransduction based on light-induced 
            homodimerization of talin.
  JOURNAL   J Cell Sci. 2025 Nov 24:jcs.264110. doi: 10.1242/jcs.264110.
  PUBMED    41277405
REFERENCE   2  (bases 1 to 12466)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Jan 7, 2026 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Cell Sci.
            2025 Nov 24:jcs.264110. doi: 10.1242/jcs.264110."
COMMENT     Sequence Label: mIFP-Talin1(1–2493)
FEATURES             Location/Qualifiers
     source          1..12466
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     polyA_signal    8123..8244
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(8160..8179)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     8214..8233
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      complement(8251..8706)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(8388..8409)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     8600..8619
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     promoter        8733..8837
                     /gene="bla"
                     /label=AmpR promoter
     promoter        8839..9196
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     enhancer        8839..9030
                     /label=SV40 enhancer
                     /note="enhancer for the SV40 early promoter (Herr, 1993)"
     primer_bind     complement(8862..8882)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     rep_origin      9047..9182
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             9231..10025
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     CDS             9231..10025
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(9285..9304)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     9895..9914
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     complement(10213..10232)
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     polyA_signal    10257..10304
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     rep_origin      10633..11221
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     11122..11141
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     enhancer        11396..11699
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        11700..11903
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     11854..11874
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     regulatory      11930..11939
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             11939..11962
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             join(11978..12466,1..471)
                     /codon_start=1
                     /product="naturally monomeric infrared fluorescent protein 
                     derived from a truncated bacteriophytochrome (Yu et al., 
                     2015)"
                     /label=mIFP
                     /translation="MSVPLTTSAFGHAFLANCEREQIHLAGSIQPHGILLAVKEPDNVV
                     IQASINAAEFLNTNSVVGRPLRDLGGDLPLQILPHLNGPLHLAPMTLRCTVGSPPRRVD
                     CTIHRPSNGGLIVELEPATKTTNIAPALDGAFHRITSSSSLMGLCDETATIIREITGYD
                     RVMVVRFDEEGNGEILSERRRADLEAFLGNRYPASTIPQIARRLYEHNRVRLLVDVNYT
                     PVPLQPRISPLNGRDLDMSLSCLRSMSPIHQKYMQDMGVGATLVCSLMVSGRLWGLIAC
                     HHYEPRFVPFHIRAAGEALAETCAIRIATLESFAQSQSK"
ORIGIN
        1 cgtgtgatgg tagtacgttt cgatgaagag ggtaatggcg aaattctgtc cgaacgtcgt
       61 cgtgcggacc tggaagcgtt cctgggtaac cgctacccgg cgtctactat tccgcagatc
      121 gctcgtcgcc tgtacgaaca taaccgtgtt cgcctgctgg tagatgtgaa ctatactccg
      181 gttccgctac agccgcgcat cagcccgctg aacggtcgtg atctggatat gtccctgtct
      241 tgcctgcgct ctatgtcccc gatccaccag aaatacatgc aggacatggg cgttggcgcg
      301 accctggttt gctctctgat ggtgtctggt cgtctgtggg gtctgatcgc ttgccaccac
      361 tacgaaccgc gcttcgttcc gttccacatt cgcgctgctg gcgaagcgct ggcggaaact
      421 tgtgcgatcc gcatcgcgac gctggagagc tttgcacagt ctcagtccaa aggcgctggg
      481 tccgggtccg ggcaagggtc cggcgcaggc tccgcgtcca tggttgcgct ttcgctgaag
      541 attagcattg ggaatgtggt gaagacgatg caatttgagc catctaccat ggtgtacgac
      601 gcctgccgca tgattcgtga gcggatccca gaggccctgg ctggccctcc caacgacttt
      661 gggctctttc tgtcagatga tgaccccaaa aaaggcatct ggctagaggc tgggaaagct
      721 ttggactact acatgctccg aaatggggac accatggagt acagaaaaaa gcagagaccc
      781 ctgaagatac ggatgttaga cggaacggtg aagactatca tggtggatga ctccaagacc
      841 gtcacagaca tgctcatgac catttgtgcc cgaattggta tcaccaacca tgatgagtat
      901 tcactggttc gagagctgat ggaagaaaag aaagatgagg ggacagggac cctgagaaaa
      961 gacaagaccc tactgcgaga tgaaaagaag atggagaagc taaagcagaa gttgcacaca
     1021 gacgatgagt tgaactggct ggaccatggg cggacactga gggaacaggg agtggaagag
     1081 catgagacgt tgctgctgcg gagaaagttc ttctactcag accagaatgt ggattcccga
     1141 gaccctgtac agctgaacct tctctatgtg caggcacgag atgacatcct gaatggctcc
     1201 catcctgtct cctttgacaa ggcctgtgaa tttgcaggct tccagtgcca gatccagttt
     1261 ggacctcaca atgaacagaa gcacaaggct ggcttccttg acctgaagga cttcctgccc
     1321 aaggagtatg tgaagcagaa gggagagcgt aagatctttc aggcacacaa gaattgtggg
     1381 cagatgagtg aaattgaggc caaggtacgc tacgtgaagc tggcccgttc cctcaagact
     1441 tacggtgtct ccttcttcct agtcaaggaa aagatgaagg ggaagaataa actggtgccc
     1501 aggctgttgg gcatcactaa ggagtgtgtg atgcgtgtgg atgagaagac caaggaggtg
     1561 attcaggaat ggagcctcac caacatcaaa cgctgggctg cctctcccaa gagctttact
     1621 ctggactttg gagactatca ggatggctac tactcagtac agacgacaga aggcgagcag
     1681 atcgcacagc tcattgctgg ctacatagat atcatcctta agaagaaaaa aagcaaggac
     1741 cattttgggc tggagggaga tgaagagtct actatgttgg aggactcagt gtctcccaaa
     1801 aagtcaacag tccttcagca gcagtacaac cgagtgggga aagtggagca cggctctgtg
     1861 gctctgccag ccatcatgcg ctctggagcc tctggtcctg agaatttcca ggtggggagc
     1921 atgccacctg cccagcagca gatcaccagt ggccagatgc accgaggaca catgccacct
     1981 ctgacttcag cccagcaggc gctcactgga accattaact ccagcatgca ggctgtgcag
     2041 gctgcccagg ccactctgga tgactttgag actctacccc ctcttggcca ggatgctgcc
     2101 tccaaagctt ggcgtaagaa caagatggat gaatcaaagc atgagatcca ctcccaggta
     2161 gatgccatca cagctggcac tgcctctgtg gtaaacctga cagcaggaga tcctgcagag
     2221 acagactata cagcagtggg ctgtgcagtc accaccatct cttccaacct gacggagatg
     2281 tcccgtgggg tgaagctatt ggctgccctg ctggaggatg aaggcggcaa tggccggccc
     2341 cttctgcaag cagcaaaggg ccttgcaggg gctgtgtcgg aactgcttcg cagtgctcag
     2401 cccgccagcg ctgagcctcg tcagaacctg ctgcaggcag ccgggaacgt gggccaggcc
     2461 agtggggagc tgttgcagca aattggggaa agtgacactg acccccactt ccaggatgtt
     2521 ctaatgcagc tagcaaacgc agtggcaagt gctgcagccg ccctggtcct caaggccaag
     2581 agtgtggccc agcgcacaga ggattccggg cttcagaccc aagtgattgc tgcagctacg
     2641 caatgtgctc tgtccacttc ccagctggtg gcctgtacca aggtggtagc acctacaatc
     2701 agctcacctg tgtgccaaga acagctagtc gaggctgggc gactggtggc caaagctgtg
     2761 gagggctgtg tgtctgcctc gcaagcagct acagaagatg gacagctgct tcgaggggta
     2821 ggagcagcag ccacggctgt cacccaggcc ctcaatgagc tgctgcagca cgtgaaggcc
     2881 cacgccaccg gagctgggcc tgccggtcgg tatgaccaag ccactgatac catcctcacc
     2941 gtcactgaaa acatcttcag ctccatgggt gatgctgggg agatggtgcg ccaggcccgc
     3001 atcctggccc aagccacctc agacctggtc aatgctatca aggccgatgc tgagggggag
     3061 agtgatctgg agaactctag aaagctcctg agtgctgcca agatcctcgc tgatgccacc
     3121 gccaagatgg tggaggcggc caagggagca gccgcccacc ctgacagtga ggaacagcag
     3181 cagcgactgc gtgaagcagc tgaggggctt cgcatggcca ccaatgcagc tgcgcagaac
     3241 gccatcaaga agaagttggt gcagcgcctg gagcatgcag ccaagcaagc tgcagcctct
     3301 gcaacacaga ccattgctgc agcccaacat gcagcctctg cccccaaggc ctctgcgggc
     3361 ccccagcccc tactggtgca gagctgtaag gccgtggcag agcagattcc gttgctggtg
     3421 cagggtgtcc gaggaagtca agctcagcct gacagcccta gtgctcagct cgccctcatt
     3481 gctgccagcc agagcttcct gcagccaggt gggaagatgg tggcagcggc aaaagcctca
     3541 gtgccaacaa ttcaggacca ggcttcagcg atgcagctga gtcagtgcgc aaagaaccta
     3601 ggcactgccc tggccgagct gcgcactgct gctcagaagg ctcaggaggc atgtggacct
     3661 ttggagatgg attctgcact gagtgttgta caaaatctag agaaagacct acaggaaata
     3721 aaagcagcag ctcgagatgg caagcttaag cccttacctg gggaaacaat ggagaagtgt
     3781 acccaagatc ttggcaacag caccaaagcg gtgagctctg ccatcgccaa gctgctgggg
     3841 gagattgccc agggcaacga gaactatgca ggtattgcag ctcgggatgt agctggtgga
     3901 ctaaggtcac tagcccaggc tgcgcggggt gtggctgcgc tgacatcaga tcctgcagtg
     3961 caggccattg tgcttgacac agccagcgac gttctggaca aggccagcag cctcattgaa
     4021 gaggcaaaaa aagcatctgg acacccaggg gacccagaaa gccagcaaag gcttgctcag
     4081 gtagctaaag cagtgaccca agccctgaac cgctgtgtca gctgcctgcc tggccaaaga
     4141 gacgtggata atgccctacg agccgttgga gatgccagca agcgactcct gagtgacttg
     4201 cttcctccaa gcacggggac atttcaagaa gcacagagcc gattgaatga agctgcagct
     4261 gggttaaatc aggccgccac agaactggtg caagcctccc gaggaactcc tcaggacctg
     4321 gctcgggcct ccggccgatt tggacaggac ttcagcacct tcctggaagc tggtgtggag
     4381 atggccggac aggccccgag ccaggaggac cgagcccagg tggtgtccaa cctgaagggc
     4441 atatccatgt cttcaagcaa acttctcctt gccgctaagg ccttgtccac agaccctgct
     4501 tctcccaacc tcaagagtca gctggctgcg gctgcccggg cagtgacgga cagcatcaac
     4561 cagctcatca ccatgtgcac ccagcaggca cctggccaga aggagtgtga caatgcactt
     4621 cggcagctag agacagtccg agaactcctg gagaatccag tccagcccat caacgacatg
     4681 tcctacttcg gttgcttgga cagtgtcatg gagaactcca aggtcctagg tgaggccatg
     4741 actggcatct cccaaaatgc caagaatgga aatctgccgg agtttggaga tgccattgcc
     4801 acagcctcca aggctctctg tggcttcacg gaggcagccg cacaggcagc atatctagtt
     4861 ggtgtctctg accccaacag ccaagctgga cagcaaggac tggtggaacc cacacagttt
     4921 gcccgtgcaa accaggcaat tcagatggcc tgtcagagtc tgggggagcc tggctgtacc
     4981 caggcccagg tgttatctgc agccactatt gtagccaaac acacatctgc attgtgtaac
     5041 agctgtcgcc tggcttccgc taggactgcc aatcccactg ccaagcgcca gtttgtacag
     5101 tcagccaagg aggtggccaa cagtacagcc aatcttgtca agaccatcaa ggcactagat
     5161 ggggacttca cagaagagaa ccgtgcccag tgccgagcag ccacagcccc tctgctggaa
     5221 gctgtggata acctgagtgc ctttgcctcc aaccctgagt tctccagcgt ccctgcccag
     5281 atcagccctg agggcagagc agccatggag cccattgtaa tctctgctaa gacaatgttg
     5341 gagagtgctg gagggctcat ccagacagct cgggcgttag cagtcaatcc ccgagacccc
     5401 ccacgttggt ctgtgctagc tggccactct cggactgtct cagattccat caagaaactt
     5461 attacaagca tgagggacaa agccccaggg cagctggagt gtgagacagc cattgcggct
     5521 ctgaacagct gcttgcggga cctagatcag gcttcgcttg ccgctgtcag ccagcagctt
     5581 gctccccgtg aaggaatctc tcaagaggct ttgcacaccc agatgctcac tgcagtgcaa
     5641 gaaatttctc atctcattga gccgctggcc agcgctgctc gggctgaagc ctcccagctg
     5701 ggacacaagg tgtcccaaat ggcccagtac tttgaaccac tcaccctggc tgcagtgggt
     5761 gctgcgtcta agaccctgag ccacccgcag cagatggcac ttctggacca gactaaaacg
     5821 ttggcagagt ctgccttgca gttgctatat actgccaagg aggctggtgg taaccccaag
     5881 caagcagcac acacccagga agccctggag gaggctgtgc agatgatgac agaggccgta
     5941 gaagacctga cgacgaccct caacgaagca gccagtgctg caggagtcgt tggcggcatg
     6001 gtggactcta tcactcaggc catcaaccag ctagatgaag gacctatggg tgacccagaa
     6061 ggctcattcg tagattacca gacaaccatg gtgaggacag ccaaggccat tgctgtcact
     6121 gttcaggaga tggtaaccaa gtcaaacacc agccctgaag agctaggccc tcttgccaac
     6181 cagctgacca gtgactatgg ccgactggcc tcacaagcca agcctgcagc tgtggctgct
     6241 gaaaatgaag agataggcgc tcacatcaaa caccgagtac aggagctggg ccatggctgc
     6301 tctgctctgg tcaccaaggc aggtgccttg cagtgtagcc ccagtgatgt ctacaccaag
     6361 aaggagctca tagagtgtgc ccgcagagtg tcagagaagg tctcccatgt tctggctgca
     6421 ctccaggctg ggaatcgtgg tacccaggcc tgcattacag cagccagtgc tgtgtctggt
     6481 atcattgctg acctcgacac caccatcatg ttcgctactg ctggcacact taaccgtgag
     6541 ggtgctgaaa cttttgctga ccaccgggag ggtatcttaa agacagcaaa ggttcttgtg
     6601 gaggacacca aggtcctagt gcagaatgca gctgggagcc aggagaagtt ggcacaagcc
     6661 gcccagtcct ccgtggccac cattacccgc ctcgctgatg tggtcaagct cggtgcagcc
     6721 agcctaggag ccgaagaccc tgaaactcag gtggtgctga tcaatgcagt aaaggacgta
     6781 gccaaggccc tgggtgacct catcagcgct acgaaggctg cagcgggcaa agttggggat
     6841 gaccctgcag tgtggcagct caagaactct gccaaggtga tggtgaccaa tgtgacatca
     6901 ttgctcaaga cagtgaaggc tgtggaagat gaggccacca aaggcacacg ggccctagag
     6961 gcaaccacag agcacatacg tcaggaactg gcggtcttct gttccccaga gccacctgcc
     7021 aagacctcta cccctgaaga cttcatccga atgaccaagg gtattactat ggcaacagcc
     7081 aaagccgttg ctgctggcaa ttcctgtcga caggaagatg tcattgccac agccaatctg
     7141 agccgacggg ctattgcgga catgcttcgg gcttgcaagg aagcagcttt ccacccagaa
     7201 gtggcgcctg atgtacggct ccgagccctg cactatggcc gggagtgtgc caatggttac
     7261 ctggaactgc tggaccacgt gctgctgacc cttcagaagc caaacccaga cctgaagcag
     7321 cagctaactg gacactcaaa gcgggttgct ggctctgtga ctgagctcat ccaggctgct
     7381 gaagccatga aaggaacaga gtgggtggac ccagaggacc ctactgtcat tgctgagaat
     7441 gagctcttgg gagccgcagc cgccatcgag gctgcagcca agaagctgga gcagctgaag
     7501 cccagggcca aacccaagga ggcggatgag tccttgaact ttgaggaaca aatcctagaa
     7561 gctgccaagt ccatcgctgc agccaccagt gcactggtaa aggctgcgtc agcagcccag
     7621 agggagctgg tggctcaagg aaaggtgggc gccattccag ccaatgcact ggatgatggg
     7681 cagtggtcgc agggcctcat ttccgctgcc cgtatggtgg ctgcggccac caacaatctg
     7741 tgtgaagcag ctaacgcagc tgtccagggc catgctagcc aggagaaact catttcctca
     7801 gccaagcaag tagctgcctc cacagctcag ctcctggtag cttgcaaggt caaagcagat
     7861 caggactctg aggcaatgaa acggctccag gctgctggca atgcagtgaa gagggcctca
     7921 gataacctgg taaaggcggc ccagaaagct gcagccttcg aagaccagga gaatgagacg
     7981 gtggtggtga aggagaaata agcggccgcg actctagatc ataatcagcc ataccacatt
     8041 tgtagaggtt ttacttgctt taaaaaacct cccacacctc cccctgaacc tgaaacataa
     8101 aatgaatgca attgttgttg ttaacttgtt tattgcagct tataatggtt acaaataaag
     8161 caatagcatc acaaatttca caaataaagc atttttttca ctgcattcta gttgtggttt
     8221 gtccaaactc atcaatgtat cttaaggcgt aaattgtaag cgttaatatt ttgttaaaat
     8281 tcgcgttaaa tttttgttaa atcagctcat tttttaacca ataggccgaa atcggcaaaa
     8341 tcccttataa atcaaaagaa tagaccgaga tagggttgag tgttgttcca gtttggaaca
     8401 agagtccact attaaagaac gtggactcca acgtcaaagg gcgaaaaacc gtctatcagg
     8461 gcgatggccc actacgtgaa ccatcaccct aatcaagttt tttggggtcg aggtgccgta
     8521 aagcactaaa tcggaaccct aaagggagcc cccgatttag agcttgacgg ggaaagccgg
     8581 cgaacgtggc gagaaaggaa gggaagaaag cgaaaggagc gggcgctagg gcgctggcaa
     8641 gtgtagcggt cacgctgcgc gtaaccacca cacccgccgc gcttaatgcg ccgctacagg
     8701 gcgcgtcagg tggcactttt cggggaaatg tgcgcggaac ccctatttgt ttatttttct
     8761 aaatacattc aaatatgtat ccgctcatga gacaataacc ctgataaatg cttcaataat
     8821 attgaaaaag gaagagtcct gaggcggaaa gaaccagctg tggaatgtgt gtcagttagg
     8881 gtgtggaaag tccccaggct ccccagcagg cagaagtatg caaagcatgc atctcaatta
     8941 gtcagcaacc aggtgtggaa agtccccagg ctccccagca ggcagaagta tgcaaagcat
     9001 gcatctcaat tagtcagcaa ccatagtccc gcccctaact ccgcccatcc cgcccctaac
     9061 tccgcccagt tccgcccatt ctccgcccca tggctgacta atttttttta tttatgcaga
     9121 ggccgaggcc gcctcggcct ctgagctatt ccagaagtag tgaggaggct tttttggagg
     9181 cctaggcttt tgcaaagatc gatcaagaga caggatgagg atcgtttcgc atgattgaac
     9241 aagatggatt gcacgcaggt tctccggccg cttgggtgga gaggctattc ggctatgact
     9301 gggcacaaca gacaatcggc tgctctgatg ccgccgtgtt ccggctgtca gcgcaggggc
     9361 gcccggttct ttttgtcaag accgacctgt ccggtgccct gaatgaactg caagacgagg
     9421 cagcgcggct atcgtggctg gccacgacgg gcgttccttg cgcagctgtg ctcgacgttg
     9481 tcactgaagc gggaagggac tggctgctat tgggcgaagt gccggggcag gatctcctgt
     9541 catctcacct tgctcctgcc gagaaagtat ccatcatggc tgatgcaatg cggcggctgc
     9601 atacgcttga tccggctacc tgcccattcg accaccaagc gaaacatcgc atcgagcgag
     9661 cacgtactcg gatggaagcc ggtcttgtcg atcaggatga tctggacgaa gagcatcagg
     9721 ggctcgcgcc agccgaactg ttcgccaggc tcaaggcgag catgcccgac ggcgaggatc
     9781 tcgtcgtgac ccatggcgat gcctgcttgc cgaatatcat ggtggaaaat ggccgctttt
     9841 ctggattcat cgactgtggc cggctgggtg tggcggaccg ctatcaggac atagcgttgg
     9901 ctacccgtga tattgctgaa gagcttggcg gcgaatgggc tgaccgcttc ctcgtgcttt
     9961 acggtatcgc cgctcccgat tcgcagcgca tcgccttcta tcgccttctt gacgagttct
    10021 tctgagcggg actctggggt tcgaaatgac cgaccaagcg acgcccaacc tgccatcacg
    10081 agatttcgat tccaccgccg ccttctatga aaggttgggc ttcggaatcg ttttccggga
    10141 cgccggctgg atgatcctcc agcgcgggga tctcatgctg gagttcttcg cccaccctag
    10201 ggggaggcta actgaaacac ggaaggagac aataccggaa ggaacccgcg ctatgacggc
    10261 aataaaaaga cagaataaaa cgcacggtgt tgggtcgttt gttcataaac gcggggttcg
    10321 gtcccagggc tggcactctg tcgatacccc accgagaccc cattggggcc aatacgcccg
    10381 cgtttcttcc ttttccccac cccacccccc aagttcgggt gaaggcccag ggctcgcagc
    10441 caacgtcggg gcggcaggcc ctgccatagc ctcaggttac tcatatatac tttagattga
    10501 tttaaaactt catttttaat ttaaaaggat ctaggtgaag atcctttttg ataatctcat
    10561 gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg tcagaccccg tagaaaagat
    10621 caaaggatct tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa
    10681 accaccgcta ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc tttttccgaa
    10741 ggtaactggc ttcagcagag cgcagatacc aaatactgtt cttctagtgt agccgtagtt
    10801 aggccaccac ttcaagaact ctgtagcacc gcctacatac ctcgctctgc taatcctgtt
    10861 accagtggct gctgccagtg gcgataagtc gtgtcttacc gggttggact caagacgata
    10921 gttaccggat aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac agcccagctt
    10981 ggagcgaacg acctacaccg aactgagata cctacagcgt gagctatgag aaagcgccac
    11041 gcttcccgaa gggagaaagg cggacaggta tccggtaagc ggcagggtcg gaacaggaga
    11101 gcgcacgagg gagcttccag ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg
    11161 ccacctctga cttgagcgtc gatttttgtg atgctcgtca ggggggcgga gcctatggaa
    11221 aaacgccagc aacgcggcct ttttacggtt cctggccttt tgctggcctt ttgctcacat
    11281 gttctttcct gcgttatccc ctgattctgt ggataaccgt attaccgcca tgcattagtt
    11341 attaatagta atcaattacg gggtcattag ttcatagccc atatatggag ttccgcgtta
    11401 cataacttac ggtaaatggc ccgcctggct gaccgcccaa cgacccccgc ccattgacgt
    11461 caataatgac gtatgttccc atagtaacgc caatagggac tttccattga cgtcaatggg
    11521 tggagtattt acggtaaact gcccacttgg cagtacatca agtgtatcat atgccaagta
    11581 cgccccctat tgacgtcaat gacggtaaat ggcccgcctg gcattatgcc cagtacatga
    11641 ccttatggga ctttcctact tggcagtaca tctacgtatt agtcatcgct attaccatgg
    11701 tgatgcggtt ttggcagtac atcaatgggc gtggatagcg gtttgactca cggggatttc
    11761 caagtctcca ccccattgac gtcaatggga gtttgttttg gcaccaaaat caacgggact
    11821 ttccaaaatg tcgtaacaac tccgccccat tgacgcaaat gggcggtagg cgtgtacggt
    11881 gggaggtcta tataagcaga gctggtttag tgaaccgtca gatccgctag ccaccatgga
    11941 ctacaaagac gatgacgaca aggggtccgg gtccgggatg tcggtaccgc tgactacctc
    12001 agcattcggc cacgcgtttc tggctaactg tgaacgcgag cagatccacc tggcgggctc
    12061 cattcagccg cacggtatcc tgctggctgt gaaagagccg gacaacgtgg tgatccaggc
    12121 ttctattaac gctgcggagt tcctgaacac caactctgtt gttggccgtc cgctgcgtga
    12181 cctgggcggc gatctgcctt tgcagatcct gccgcacctg aacggcccgc tgcacctggc
    12241 tccgatgacc ctgcgttgta ccgtgggttc tccgccgcgt cgtgtggact gtaccattca
    12301 tcgtccgtct aacggcggcc tgatcgtaga actggaacca gcaaccaaga ccactaacat
    12361 tgcgccggct ctggacggtg cgtttcatcg tatcacttct tcatcctccc tgatgggcct
    12421 gtgtgacgaa accgcgacta ttatccgtga gattactggc tacgac
//
