LOCUS       sequence-496378-        8453 bp    DNA     circular SYN 07-JAN-2026
DEFINITION  Yeast 2-hybrid vector encoding human heterotrimeric G protein GNAO1 
            fused to the GAL4 DNA binding domain..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8453)
  TITLE     Assman lab Y2H vectors
REFERENCE   2  (bases 1 to 8453)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Jan 7, 2026 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pDEST-GBKT7-GNAO1
FEATURES             Location/Qualifiers
     source          1..8453
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        30..734
                     /gene="S. cerevisiae ADH1"
                     /label=ADH1 promoter
                     /note="promoter for alcohol dehydrogenase 1"
     CDS             762..1202
                     /codon_start=1
                     /gene="Saccharomyces cerevisiae GAL4 (truncated)"
                     /product="DNA binding domain of the GAL4 transcriptional 
                     activator"
                     /label=GAL4 DNA binding domain
                     /translation="MKLLSSIEQACDICRLKKLKCSKEKPKCAKCLKNNWECRYSPKTK
                     RSPLTRAHLTEVESRLERLEQLFLLIFPREDLDMILKMDSLQDIKALLTGLFVQDNVNK
                     DAVTDRLASVETDMPLTLRQHRISATSSSEESSNKGQRQLTVS"
     primer_bind     1213..1232
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        1213..1231
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             1251..1280
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     protein_bind    1311..1335
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(2428..2452)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     terminator      2488..2535
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     terminator      2562..2749
                     /gene="S. cerevisiae ADH1"
                     /label=ADH1 terminator
                     /note="transcription terminator for the S. cerevisiae 
                     alcohol dehydrogenase 1 (ADH1) gene"
     primer_bind     complement(2617..2641)
                     /label=pHybLex-R
                     /note="ADH terminator, reverse primer"
     primer_bind     complement(2773..2789)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2773..2789)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(2786..2808)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    2797..2813
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(2821..2827,2828..2845,2846..2851))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    2866..2887
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(3004..3021)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(3175..3763)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(3255..3274)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     polyA_signal    4092..4139
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     primer_bind     4164..4183
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     CDS             complement(4371..5165)
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     CDS             complement(4371..5165)
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(4482..4501)
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     5092..5111
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     promoter        complement(5166..5270)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      5297..6639
                     /label=2u ori
                     /note="yeast 2u plasmid origin of replication"
     protein_bind    complement(6260..6307)
                     /label=FRT
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="FLP-mediated recombination occurs in the 8-bp core 
                     sequence TCTAGAAA (Turan and Bode, 2011)."
     primer_bind     6680..6698
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(6736..6758)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     6858..6877
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     promoter        6898..7179
                     /gene="S. cerevisiae TRP1"
                     /label=TRP1 promoter
     CDS             7180..7854
                     /codon_start=1
                     /gene="S. cerevisiae TRP1"
                     /product="phosphoribosylanthranilate isomerase, required 
                     for tryptophan biosynthesis"
                     /label=TRP1
                     /note="yeast auxotrophic marker"
                     /translation="MSVINFTGSSGPLVKVCGLQSTEAAECALDSDADLLGIICVPNRK
                     RTIDPVIARKISSLVKAYKNSSGTPKYLVGVFRNQPKEDVLALVNDYGIDIVQLHGDES
                     WQEYQEFLGLPVIKRLVFPKDCNILLSAASQKPHSFIPLFDSEAGGTGELLDWNSISDW
                     VGRQESPESLHFMLAGGLTPENVGDALRLNGVIGVDVSGGVETNGVKDSNKIANFVKNA
                     KK"
     rep_origin      complement(7957..8412)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(8094..8115)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     8306..8325
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
ORIGIN
        1 cggtgcgggc ctcttcgcta ttacgccaga tccttttgtt gtttccgggt gtacaatatg
       61 gacttcctct tttctggcaa ccaaacccat acatcgggat tcctataata ccttcgttgg
      121 tctccctaac atgtaggtgg cggaggggag atatacaata gaacagatac cagacaagac
      181 ataatgggct aaacaagact acaccaatta cactgcctca ttgatggtgg tacataacga
      241 actaatactg tagccctaga cttgatagcc atcatcatat cgaagtttca ctaccctttt
      301 tccatttgcc atctattgaa gtaataatag gcgcatgcaa cttcttttct ttttttttct
      361 tttctctctc ccccgttgtt gtctcaccat atccgcaatg acaaaaaaat gatggaagac
      421 actaaaggaa aaaattaacg acaaagacag caccaacaga tgtcgttgtt ccagagctga
      481 tgaggggtat ctcgaagcac acgaaacttt ttccttcctt cattcacgca cactactctc
      541 taatgagcaa cggtatacgg ccttccttcc agttacttga atttgaaata aaaaaaagtt
      601 tgctgtcttg ctatcaagta taaatagacc tgcaattatt aatcttttgt ttcctcgtca
      661 ttgttctcgt tccctttctt ccttgtttct ttttctgcac aatatttcaa gctataccaa
      721 gcatacaatc aactccaagc ttgaagcaag cctcctgaaa gatgaagcta ctgtcttcta
      781 tcgaacaagc atgcgatatt tgccgactta aaaagctcaa gtgctccaaa gaaaaaccga
      841 agtgcgccaa gtgtctgaag aacaactggg agtgtcgcta ctctcccaaa accaaaaggt
      901 ctccgctgac tagggcacat ctgacagaag tggaatcaag gctagaaaga ctggaacagc
      961 tatttctact gatttttcct cgagaagacc ttgacatgat tttgaaaatg gattctttac
     1021 aggatataaa agcattgtta acaggattat ttgtacaaga taatgtgaat aaagatgccg
     1081 tcacagatag attggcttca gtggagactg atatgcctct aacattgaga cagcatagaa
     1141 taagtgcgac atcatcatcg gaagagagta gtaacaaagg tcaaagacag ttgactgtat
     1201 cgccggaatt tgtaatacga ctcactatag ggcgagccgc catcatggag gagcagaagc
     1261 tgatctcaga ggaggacctg catatggcca tggaggccga attcccatca acaagtttgt
     1321 acaaaaaagc aggctccgaa ttcgccctta tgggatgtac tctgagcgca gaggagagag
     1381 ccgccctcga gcggagcaag gcgattgaga aaaacctcaa agaggatggc atcagcgccg
     1441 ccaaagacgt gaaattactc ctgctcgggg ctggagaatc aggaaaaagc accattgtga
     1501 agcagatgaa gatcatccat gaagatggct tctccggaga agacgtgaaa cagtacaagc
     1561 ctgttgtcta cagcaacact atccagtccc tggcagccat cgtccgggcc atggacactt
     1621 tgggcatcga atatggtgat aaggagagaa aggctgacgc caagatggtg tgtgatgtgg
     1681 tgagtcggat ggaagacacc gagcccttct ctgcagagct gctttctgcc atgatgcggc
     1741 tctggggcga ctcaggaatc caagagtgct tcaaccggtc ccgggagtat cagctcaacg
     1801 actctgccaa atactacctg gacagcctgg atcggattgg ggccgccgac taccagccca
     1861 ccgagcagga catcctccga accagggtca aaaccactgg catcgtagaa acccacttca
     1921 cattcaagaa cctccacttc aggctgtttg acgtcggagg ccagcgatct gaacgcaaga
     1981 agtggatcca ttgcttcgag gacgtcacgg ccatcatttt ctgtgtcgcg ctcagcggct
     2041 atgaccaggt gctccacgaa gacgaaacca cgaaccgcat gcacgagtct ctcatgctct
     2101 tcgactccat ctgtaacaac aagttcttca tcgatacctc catcattctc ttcctcaaca
     2161 agaaagatct ctttggcgag aagatcaaga agtcaccttt gaccatctgc tttcctgaat
     2221 acacaggccc caatacctat gaagacgcag ccgcctacat ccaagcacaa tttgaaagca
     2281 aaaaccgctc acccaacaaa gaaatatatt gtcacatgac ttgtgccaca gacacgaata
     2341 acatccaggt ggtgttcgac gccgtcaccg acatcatcat tgccaacaac ctccggggct
     2401 gcggcttgta ctgaaagggc gaattcgacc cagctttctt gtacaaagtg gttgatgggg
     2461 atccgtcgac ctgcagcggc cgcataacta gcataacccc ttggggcctc taaacgggtc
     2521 ttgaggggtt ttttgcgcgc ttgcagccaa gctaattccg ggcgaatttc ttatgattta
     2581 tgatttttat tattaaataa gttataaaaa aaataagtgt atacaaattt taaagtgact
     2641 cttaggtttt aaaacgaaaa ttcttattct tgagtaactc tttcctgtag gtcaggttgc
     2701 tttctcaggt atagcatgag gtcgctctta ttgaccacac ctctaccggc atgcaagctt
     2761 ggcgtaatca tggtcatagc tgtttcctgt gtgaaattgt tatccgctca caattccaca
     2821 caacatacga gccggaagca taaagtgtaa agcctggggt gcctaatgag tgagctaact
     2881 cacattaatt gcgttgcgct cactgcccgc tttccagtcg ggaaacctgt cgtgccagct
     2941 gcattaatga atcggccaac gcgcggggag aggcggtttg cgtattgggc gctcttccgc
     3001 ttcctcgctc actgactcgc tgcgctcggt cgttcggctg cggcgagcgg tatcagctca
     3061 ctcaaaggcg gtaatacggt tatccacaga atcaggggat aacgcaggaa agaacatgtg
     3121 agcaaaaggc cagcaaaagg ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca
     3181 taggctccgc ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa
     3241 cccgacagga ctataaagat accaggcgtt tccccctgga agctccctcg tgcgctctcc
     3301 tgttccgacc ctgccgctta ccggatacct gtccgccttt ctcccttcgg gaagcgtggc
     3361 gctttctcat agctcacgct gtaggtatct cagttcggtg taggtcgttc gctccaagct
     3421 gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc gccttatccg gtaactatcg
     3481 tcttgagtcc aacccggtaa gacacgactt atcgccactg gcagcagcca ctggtaacag
     3541 gattagcaga gcgaggtatg taggcggtgc tacagagttc ttgaagtggt ggcctaacta
     3601 cggctacact agaagaacag tatttggtat ctgcgctctg ctgaagccag ttaccttcgg
     3661 aaaaagagtt ggtagctctt gatccggcaa acaaaccacc gctggtagcg gtggtttttt
     3721 tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct caagaagatc ctttgatctt
     3781 ttctacgggg tctgacgctc agtggaacga aaactcacgt taagggattt tggtcatgag
     3841 attatcaaaa aggatcttca cctagatcct tttaaattaa aaatgaagtt ttaaatcaat
     3901 ctaaagtata tatgagtaac ctgaggctat ggcagggcct gccgccccga cgttggctgc
     3961 gagccctggg ccttcacccg aacttggggg gtggggtggg gaaaaggaag aaacgcgggc
     4021 gtattggccc caatggggtc tcggtggggt atcgacagag tgccagccct gggaccgaac
     4081 cccgcgttta tgaacaaacg acccaacacc gtgcgtttta ttctgtcttt ttattgccgt
     4141 catagcgcgg gttccttccg gtattgtctc cttccgtgtt tcagttagcc tccccctagg
     4201 gtgggcgaag aactccagca tgagatcccc gcgctggagg atcatccagc cggcgtcccg
     4261 gaaaacgatt ccgaagccca acctttcata gaaggcggcg gtggaatcga aatctcgtga
     4321 tggcaggttg ggcgtcgctt ggtcggtcat ttcgaacccc agagtcccgc tcagaagaac
     4381 tcgtcaagaa ggcgatagaa ggcgatgcgc tgcgaatcgg gagcggcgat accgtaaagc
     4441 acgaggaagc ggtcagccca ttcgccgcca agctcttcag caatatcacg ggtagccaac
     4501 gctatgtcct gatagcggtc cgccacaccc agccggccac agtcgatgaa tccagaaaag
     4561 cggccatttt ccaccatgat attcggcaag caggcatcgc catgagtcac gacgagatcc
     4621 tcgccgtcgg gcatgctcgc cttgagcctg gcgaacagtt cggctggcgc gagcccctga
     4681 tgctcttcgt ccagatcatc ctgatcgaca agaccggctt ccatccgagt acgtgctcgc
     4741 tcgatgcgat gtttcgcttg gtggtcgaat gggcaggtag ccggatcaag cgtatgcagc
     4801 cgccgcattg catcagccat gatggatact ttctcggcag gagcaaggtg agatgacagg
     4861 agatcctgcc ccggcacttc gcccaatagc agccagtccc ttcccgcttc agtgacaacg
     4921 tcgagcacag ctgcgcaagg aacgcccgtc gtggccagcc acgatagccg cgctgcctcg
     4981 tcttgcagtt cattcagggc accggacagg tcggtcttga caaaaagaac cgggcgcccc
     5041 tgcgctgaca gccggaacac ggcggcatca gagcagccga ttgtctgttg tgcccagtca
     5101 tagccgaata gcctctccac ccaagcggcc ggagaacctg cgtgcaatcc atcttgttca
     5161 atcatactct tcctttttca atattattga agcatttatc agggttattg tctcatgagc
     5221 ggatacatat ttgaatgtat ttagaaaaat aaacaaatag gggttccgcg cacatttccc
     5281 cgaaaagtgc cacctgaacg aagcatctgt gcttcatttt gtagaacaaa aatgcaacgc
     5341 gagagcgcta atttttcaaa caaagaatct gagctgcatt tttacagaac agaaatgcaa
     5401 cgcgaaagcg ctattttacc aacgaagaat ctgtgcttca tttttgtaaa acaaaaatgc
     5461 aacgcgagag cgctaatttt tcaaacaaag aatctgagct gcatttttac agaacagaaa
     5521 tgcaacgcga gagcgctatt ttaccaacaa agaatctata cttctttttt gttctacaaa
     5581 aatgcatccc gagagcgcta tttttctaac aaagcatctt agattacttt ttttctcctt
     5641 tgtgcgctct ataatgcagt ctcttgataa ctttttgcac tgtaggtccg ttaaggttag
     5701 aagaaggcta ctttggtgtc tattttctct tccataaaaa aagcctgact ccacttcccg
     5761 cgtttactga ttactagcga agctgcgggt gcattttttc aagataaagg catccccgat
     5821 tatattctat accgatgtgg attgcgcata ctttgtgaac agaaagtgat agcgttgatg
     5881 attcttcatt ggtcagaaaa ttatgaacgg tttcttctat tttgtctcta tatactacgt
     5941 ataggaaatg tttacatttt cgtattgttt tcgattcact ctatgaatag ttcttactac
     6001 aatttttttg tctaaagagt aatactagag ataaacataa aaaatgtaga ggtcgagttt
     6061 agatgcaagt tcaaggagcg aaaggtggat gggtaggtta tatagggata tagcacagag
     6121 atatatagca aagagatact tttgagcaat gtttgtggaa gcggtattcg caatatttta
     6181 gtagctcgtt acagtccggt gcgtttttgg ttttttgaaa gtgcgtcttc agagcgcttt
     6241 tggttttcaa aagcgctctg aagttcctat actttctaga gaataggaac ttcggaatag
     6301 gaacttcaaa gcgtttccga aaacgagcgc ttccgaaaat gcaacgcgag ctgcgcacat
     6361 acagctcact gttcacgtcg cacctatatc tgcgtgttgc ctgtatatat atatacatga
     6421 gaagaacggc atagtgcgtg tttatgctta aatgcgtact tatatgcgtc tatttatgta
     6481 ggatgaaagg tagtctagta cctcctgtga tattatccca ttccatgcgg ggtatcgtat
     6541 gcttccttca gcactaccct ttagctgttc tatatgctgc cactcctcaa ttggattagt
     6601 ctcatccttc aatgctatca tttcctttga tattggatca tactaagaaa ccattattat
     6661 catgacatta acctataaaa ataggcgtat cacgaggccc tttcgtctcg cgcgtttcgg
     6721 tgatgacggt gaaaacctct gacacatgca gctcccggag acggtcacag cttgtctgta
     6781 agcggatgcc gggagcagac aagcccgtca gggcgcgtca gcgggtgttg gcgggtgtcg
     6841 gggctggctt aactatgcgg catcagagca gattgtactg agagtgcacc atagatcaac
     6901 gacattacta tatatataat ataggaagca tttaatagaa cagcatcgta atatatgtgt
     6961 actttgcagt tatgacgcca gatggcagta gtggaagata ttctttattg aaaaatagct
     7021 tgtcacctta cgtacaatct tgatccggag cttttctttt tttgccgatt aagaattaat
     7081 tcggtcgaaa aaagaaaagg agagggccaa gagggagggc attggtgact attgagcacg
     7141 tgagtatacg tgattaagca cacaaaggca gcttggagta tgtctgttat taatttcaca
     7201 ggtagttctg gtccattggt gaaagtttgc ggcttgcaga gcacagaggc cgcagaatgt
     7261 gctctagatt ccgatgctga cttgctgggt attatatgtg tgcccaatag aaagagaaca
     7321 attgacccgg ttattgcaag gaaaatttca agtcttgtaa aagcatataa aaatagttca
     7381 ggcactccga aatacttggt tggcgtgttt cgtaatcaac ctaaggagga tgttttggct
     7441 ctggtcaatg attacggcat tgatatcgtc caactgcatg gagatgagtc gtggcaagaa
     7501 taccaagagt tcctcggttt gccagttatt aaaagactcg tatttccaaa agactgcaac
     7561 atactactca gtgcagcttc acagaaacct cattcgttta ttcccttgtt tgattcagaa
     7621 gcaggtggga caggtgaact tttggattgg aactcgattt ctgactgggt tggaaggcaa
     7681 gagagccccg aaagcttaca ttttatgtta gctggtggac tgacgccaga aaatgttggt
     7741 gatgcgctta gattaaatgg cgttattggt gttgatgtaa gcggaggtgt ggagacaaat
     7801 ggtgtaaaag actctaacaa aatagcaaat ttcgtcaaaa atgctaagaa ataggttatt
     7861 actgagtagt atttatttaa gtattgtttg tgcacttgcc gatctatgcg gtgtgaaata
     7921 ccgcacagat gcgtaaggag aaaataccgc atcaggaaat tgtaagcgtt aatattttgt
     7981 taaaattcgc gttaaatttt tgttaaatca gctcattttt taaccaatag gccgaaatcg
     8041 gcaaaatccc ttataaatca aaagaataga ccgagatagg gttgagtgtt gttccagttt
     8101 ggaacaagag tccactatta aagaacgtgg actccaacgt caaagggcga aaaaccgtct
     8161 atcagggcga tggcccacta cgtgaaccat caccctaatc aagttttttg gggtcgaggt
     8221 gccgtaaagc actaaatcgg aaccctaaag ggagcccccg atttagagct tgacggggaa
     8281 agccggcgaa cgtggcgaga aaggaaggga agaaagcgaa aggagcgggc gctagggcgc
     8341 tggcaagtgt agcggtcacg ctgcgcgtaa ccaccacacc cgccgcgctt aatgcgccgc
     8401 tacagggcgc gtccattcgc cattcaggct gcgcaactgt tgggaagggc gat
//
