LOCUS       sequence-496389-        8214 bp    DNA     circular SYN 07-JAN-2026
DEFINITION  PBT0779 biosensor circuit (pPhlF133 version) in pNBU2 integrative 
            Bacteroides plasmid.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8214)
  TITLE     Tropini Lab plasmids
REFERENCE   2  (bases 1 to 8214)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Jan 7, 2026 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pEM667
FEATURES             Location/Qualifiers
     source          1..8214
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             207..893
                     /codon_start=1
                     /product="bright cysteine-free monomeric red fluorescent 
                     protein with fast and complete maturation (Gadella et al., 
                     2023)"
                     /label=mScarlet3
                     /translation="MDSTEAVIKEFMRFKVHMEGSMNGHEFEIEGEGEGRPYEGTQTAK
                     LKVTKGGPLPFSWDILSPQFMYGSRAFIKHPADIPDYWKQSFPEGFKWERVMIFEDGGT
                     VSVTQDTSLEDGTLIYKVKLRGGNFPPDGPVMQKRTMGWEASTERLYPEDVVLKGDIKM
                     ALRLKDGGRYLADFKTTYKAKKPVQMPGAFNIDRKLDITSHNEDYTVVEQYERSVARHS
                     TGGSGGS"
     primer_bind     complement(337..355)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     terminator      919..946
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      2100..2171
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     terminator      2187..2214
                     /label=T7Te terminator
                     /note="phage T7 early transcription terminator"
     CDS             join(2510..2512,2513..2515,2516..3229)
                     /codon_start=1
                     /product="monomeric GFP derivative that displays 
                     exceptionally high brightness in cells (Campbell et al., 
                     2020)"
                     /label=mGreenLantern
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVRGEGEGDATNGKLTL
                     KFICTTGKLPVPWPTLVTTLGYGVACFARYPDHMKQHDFFKSAMPEGYVQERTISFKDD
                     GTYKTRAEVKFEGDTLVNRIVLKGIDFKEDGNILGHKLEYNFNSHKVYITADKQKNGIK
                     ANFKTRHNVEDGGVQLADHYQQNTPIGDGPVLLPDNHYLSHQSKLSKDPNEKRDHMVLK
                     ERVTAAGITHDMDELYK"
     primer_bind     complement(2555..2576)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     terminator      3250..3277
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     CDS             complement(join(5257..6048,6049..6117))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     5880..5899
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      7409..7797
                     /label=R6K gamma ori
                     /note="gamma replication origin from E. coli plasmid R6K; 
                     requires the R6K initiator protein pi for replication"
     oriT            7968..8077
                     /note="incP origin of transfer"
ORIGIN
        1 caattgggct accttttttt tgttttgttt gcaatggtta atctattgtt aaaatttaaa
       61 gtttcacttg aactttcaaa taatgttctt atatttgcag tgtcgaaaga aacaaagtag
      121 gactgatcac acaacattta aaaaataaca ttatgaaagc actcgaaaag agattcaaag
      181 gtaatattaa caataattta ttttcaatgg atagcaccga ggcagtgatc aaggagttca
      241 tgcggttcaa ggtgcacatg gagggctcca tgaacggcca cgagttcgag atcgagggcg
      301 agggcgaggg ccgcccctac gagggcaccc agaccgccaa gctgaaggtg accaagggtg
      361 gccccctgcc cttctcctgg gacatcctgt cccctcagtt catgtacggc tccagggcct
      421 tcatcaagca ccccgccgac atccccgact actggaagca gtccttcccc gagggcttca
      481 agtgggagcg cgtgatgatc ttcgaggacg gcggcaccgt gtccgtgacc caggacacct
      541 ccctggagga cggcaccctg atctacaagg tgaagctccg cggcggcaac ttccctcctg
      601 acggccccgt aatgcagaag aggacaatgg gctgggaagc atccaccgag cggttgtacc
      661 ccgaggacgt cgtgctgaag ggcgacatta agatggccct gcgcctgaag gacggcggcc
      721 gctacctggc ggacttcaag accacctaca aggccaagaa gcccgtgcag atgcccggcg
      781 ccttcaacat cgaccgcaag ttggacatca cctcccacaa cgaggactac accgtggtgg
      841 aacagtacga acgctccgtg gcccgccact ccaccggcgg ctccggtggc tcctaataaa
      901 tcctagctga ttagctcgag aaggccatcc tgacggatgg cctttttttt gtatggggac
      961 tctgagaacc ctacctttca aaccccgagg cagcatccat tgcctgaaaa ataaccagaa
     1021 ccgagtagcg gtcttgtgca taaagcatac tttgcagttc tgacagcttt tcgatatttt
     1081 gggctaactg ttctttggca tcctgtttgg acatatcagc agtaaatgag ggattgaaat
     1141 cggatacatg atgttccttc cccggtttgg ctgtaagctc ttttaagact tcttttttca
     1201 ttgttgtata attaggttgt tataggtata taacagttga ataaagaaat cagttcttgc
     1261 caaattaaga gcgttgaata gtaattcccc aattatgtat caacagattc tacataattc
     1321 cacactaatt ccacaattat atttatattg tctgctgttt atatgtttga ttatcagtta
     1381 tatatggcgg agtgaaacgt ttggcatctg ctttgctata tattaagtac aagaaacaat
     1441 agttctatta ttcacactta aaaaattacg attatggcac gtaccccgag ccgtagcagc
     1501 attggtagcc tgcgtagtcc gcatacccat aaagcaattc tgaccagcac cattgaaatc
     1561 ctgaaagaat gtggttatag cggtctgagc attgaaagcg ttgcacgtcg tgccggtgca
     1621 agcaaaccga ccatttatcg ttggtggacc aataaagcag cactgattgc cgaagtgtat
     1681 gaaaatgaaa gcgaacaggt gcgtaaattt ccggatctgg gtagctttaa agccgatctg
     1741 gattttctgc tgcgtaatct gtggaaagtt tggcgtgaaa ccatttgtgg tgaagcattt
     1801 cgttgtgtta ttgcagaagc acagctggac cctgcaaccc tgacccagct gaaagatcag
     1861 tttatggaac gtcgtcgtga gatgccgaaa aaactggttg aaaatgccat tagcaatggt
     1921 gaactgccga aagataccaa tcgtgaactg ctgctggata tgatttttgg tttttgttgg
     1981 tatcgcctgc tgaccgaaca gctgaccgtt gaacaggata ttgaagaatt taccttcctg
     2041 ctgattaatg gtgtttgtcc gggtacacag cgttaactag ggcccatacc cccaggcatc
     2101 aaataaaacg aaaggctcag tcgaaagact gggcctttcg ttttatctgt tgtttgtcgg
     2161 tgaacgctct ctactagagt cacactggct caccttcggg tgggcctttc tgcgtttata
     2221 cgacgtacgg tggaatctga ttcgttacca attgacatga tacgaaacgt accgtatcgg
     2281 gtattcgagc attacctcat actttggatt tttttttgtt ttgtttgcaa tggttaatct
     2341 attgttaaat gatacgaaac gtaccgtatc gttaaggtat gttcttatat ttgcagtatg
     2401 atacgaaacg taccgtatcg ttaaggtgat cacacaacat ttaaaaaata acattatgaa
     2461 agcactcgaa aagagattca aaggtaatat taacaataat ttattttcaa tggtgagcaa
     2521 gggcgaggag ctgttcaccg gggtggtgcc catcctggtc gagctggacg gcgacgtaaa
     2581 cggccacaag ttcagcgtcc gcggcgaggg cgagggcgat gccaccaacg gcaagctgac
     2641 cctgaagttc atctgcacca ccggcaagct gcccgtgccc tggcccaccc tcgtgaccac
     2701 cttaggctac ggcgtggcct gcttcgcccg ctaccccgac cacatgaagc agcacgactt
     2761 cttcaagtcc gccatgcccg aaggctacgt ccaggagcgc accatctctt tcaaggacga
     2821 cggtacctac aagacccgcg ccgaggtgaa gttcgagggc gacaccctgg tgaaccgcat
     2881 cgtgctgaag ggcatcgact tcaaggagga cggcaacatc ctggggcaca agctggagta
     2941 caacttcaac agccacaagg tctatatcac ggccgacaag cagaagaacg gcatcaaggc
     3001 taacttcaag acccgccaca acgttgagga cggcggcgtg cagctcgccg accactacca
     3061 gcagaacacc cccatcggcg acggccccgt gctgctgccc gacaaccact acctgagcca
     3121 tcagtccaag ctgagcaaag accccaacga gaagcgcgat cacatggtcc tgaaggagag
     3181 ggtgaccgcc gccgggatta cacatgacat ggacgagctg tacaagtaat agcgttcagc
     3241 tcgactgtga gaaggccatc ctgacggatg gccttttttt ttcgttataa ctagttgcgt
     3301 tcctagccgc tactagtgta attgcctatc ttccagtgat ggaacagcat ttgtgcattg
     3361 gctgcaacaa tcagccttac ttgtgcctgt tctatttccg aaccgaccgc ttgtatgaat
     3421 gcatcaaaat tcgttttctc tacgttggat tccttgttgc tcatattgtg atgataattt
     3481 ctacaaatat agtcattggt aactatctat gaaactgttt gatactttta tagttgatta
     3541 aacttgttca tggcatttgc cttaatatca tccgctatgt caatgtaggg tttcatagct
     3601 ttgtagtcgc tgtgtcccgt ccatttcatg accacctgtg ccgggattcc gagagccagc
     3661 gcattgcaga tgaatgtcct ttttcctgca tgggtactga gcaaagcgta tttgtgtgtg
     3721 acttcatcaa tacgttcatt tcccttgtag taggtttccc gtacaggctc gttgatttct
     3781 gccagttcgc ccagctcttt caggtaatcg ttcatcttct ggttgctgat gacgggcaga
     3841 gccatgtaat tctcgaaatg gatgtccttg tatttgtcca gtatggcttt gctgtatttg
     3901 ttcagttcaa tcgtcaggct gtcggcagtc ttgactgtgg ttatttcgat gtggtcggac
     3961 ttcacatcgc ttcttttcag attgcgaaca tccgaatacc gcaaactcgt aaagcagcag
     4021 aacaggaaaa catcacgcac acgttccagg tattgcttat ccttgggtat ctggtagtct
     4081 ttcagcttgt tcagttcatc ccaagtcagg aagattactt ttttcgaggt ggttttcagt
     4141 ttcggtttga acgtatcgta tgcaatgttc tgatgatgtc ctttcttgaa gctccagcgc
     4201 aggaaccatt tgaggaatcc catttgcttg ccgatggtgc tgtttctcat atccttggtg
     4261 tcacgcagga agttgacgta ttcgttcaat ccaaactcgt tgaaatagtt gaacgttgca
     4321 tcctccttga actctttgag gtggttcctc actgctgcaa atttttcata ggtggatgcc
     4381 gtccagttat tctggttacc gcactctttt acaaactcat cgaacacctc ccaaaagctg
     4441 acaggggctt cttccggctg ttcttcactg gtgtctttca ttctcatgtt gaaagcttcc
     4501 ttcaactgtt gggtcgttgg catgacctcc tgcacctcaa attccttgaa aatattctgg
     4561 atttcggcat agtatttcag caagtccgta ttgatttcgg ctgcactttg ctttagcttg
     4621 ttggtacatc cgttctttac ccgctgctta tctgcatccc atttggctac gtcaatccgg
     4681 tagcccgttg taaactcgat acgttggctg gcaaagatga cacgcatacg gatgggtacg
     4741 ttctctacga ttggcacacc gttctttttc cggctctcca atgcaaaaat gatgttgcgc
     4801 ttgatattca taattgggtg cgtttgaaat tctacaccca aatatacacc caattattga
     4861 gatagcaaaa gacatttaga aacatttact tttactctat attgtaattt acacttgatt
     4921 atcagtcgtt tgcagtttta tgatattctg tgaaagtata agttcgagag cctgtctctc
     4981 cgcaaaaaac gctgaaaatc agcagattgc aaaacaaaca ccctgtttta cacccaagaa
     5041 tgtaaagtcg ggtgtttttg ttttatttaa gataatacaa ccactacata ataaaagagt
     5101 agcgatatta aaagaatccg atgagaaaag actaatattt atctatccat tcagtttgat
     5161 ttctcaggac tttacatcgt cctgaaagta tttgttggta ccggtaccga ggacgcgaca
     5221 gctgcagacg cgttatgagt aaacttggtc tgacagttac caatgcttaa tcagtgaggc
     5281 acctatctca gcgatctgtc tatttcgttc atccatagtt gcctgactcc ccgtcgtgta
     5341 gataactacg atacgggagg gcttaccatc tggccccagt gctgcaatga taccgcggga
     5401 cccacgctca ccggctccag atttatcagc aataaaccag ccagccggaa gggccgagcg
     5461 cagaagtggt cctgcaactt tatccgcctc catccagtct attaattgtt gccgggaagc
     5521 tagagtaagt agttcgccag ttaatagttt gcgcaacgtt gttgccattg ctacaggcat
     5581 cgtggtgtca cgctcgtcgt ttggtatggc ttcattcagc tccggttccc aacgatcaag
     5641 gcgagttaca tgatccccca tgttgtgcaa aaaagcggtt agctccttcg gtcctccgat
     5701 cgttgtcaga agtaagttgg ccgcagtgtt atcactcatg gttatggcag cactgcataa
     5761 ttctcttact gtcatgccat ccgtaagatg cttttctgtg actggtgagt actcaaccaa
     5821 gtcattctga gaatagtgta tgcggcgacc gagttgctct tgcccggcgt caatacggga
     5881 taataccgcg ccacatagca gaactttaaa agtgctcatc attggaaaac gttcttcggg
     5941 gcgaaaactc tcaaggatct taccgctgtt gagatccagt tcgatgtaac ccactcgtgc
     6001 acccaactga tcttcagcat cttttacttt caccagcgtt tctgggtgag caaaaacagg
     6061 aaggcaaaat gccgcaaaaa agggaataag ggcgacacgg aaatgttgaa tactcatact
     6121 cttccttttt caatcatggc cgcgggatta aaagtcgggg attggtgaac aaaaaggtgt
     6181 ttctctcttt aagagaaata tcgttttgct aaacagttga tattgaggta tcattttatc
     6241 gtaaaagaca tttttgctca acaattgctt gacggaaatc aacaaatttt agcattttgt
     6301 aaaaaagtcg ctatataatt tggtgaattg gagttatttt catatttttg catcccgaag
     6361 agtttctctt aaagagagaa acatcttttg catacctttt ccgaccgaat ttttatgtcg
     6421 taaagagggg ctttgcaggg ggtggactca gaaagatgag aatagatgac tattgtagtt
     6481 gaaacacata gaaagttgct gatatacaga ccgatacgca tatcgggatg aaccatgagt
     6541 acgttctttt ctcaaaaaac ataaatattc gaaaagagat gcaataaatt aaggagaggt
     6601 tataatgaac aaagtaaata taaaagatag tcaaaatttt attacttcaa aatatcacat
     6661 agaaaaaata atgaattgca taagtttaga tgaaaaagat aacatctttg aaataggtgc
     6721 agggaaaggt cattttactg ctggattggt aaagagatgt aattttgtaa cggcgataga
     6781 aattgattct aaattatgtg aggtaactcg taataagctc ttaaattatc ctaactatca
     6841 aatagtaaat gatgatatac tgaaatttac atttcctagc cacaatccat ataaaatatt
     6901 tggcaacata ccttacaaca taagcacaaa tataattcga aaaattgttt ttgaaagttc
     6961 agccacaata agttatttaa tagtggaata tggttttgct aaaatgttat tagatacaaa
     7021 cagatcacta gcattgctgt taatggcaga ggtagatatt tctatattag caaaaattcc
     7081 taggtattat ttccatccaa aacctaaagt ggatagcaca ttaattgtat taaaaagaaa
     7141 gccagcaaaa atggcattta aagagagaaa aaaatatgaa acttttgtaa tgaaatgggt
     7201 taacaaagag tacgaaaaac tgtttacaaa aaatcaattt aataaagctt taaaacatgc
     7261 gagaatatat gatataaaca atattagttt cgaacaattt gtatcgctat ttaatagtta
     7321 taaaatattt aacggctaaa aacaataggc cacatgcaac tgtaaatgtt tacgcgggta
     7381 ccgacaccgc ggtggagggg aattcccatg tcagccgtta agtgttcctg tgtcactcaa
     7441 aattgctttg agaggctcta agggcttctc agtgcgttac atccctggct tgttgtccac
     7501 aaccgttaaa ccttaaaagc tttaaaagcc ttatatattc ttttttttct tataaaactt
     7561 aaaaccttag aggctattta agttgctgat ttatattaat tttattgttc aaacatgaga
     7621 gcttagtacg tgaaacatga gagcttagta cgttagccat gagagcttag tacgttagcc
     7681 atgagggttt agttcgttaa acatgagagc ttagtacgtt aaacatgaga gcttagtacg
     7741 tgaaacatga gagcttagta cgtactatca acaggttgaa ctgctgatct tcagatcctc
     7801 tacgccggac gcatcgtggc cggatcaatt ccgttttccg ctgcataacc ctgcttcggg
     7861 gtcattatag cgattttttc ggtatatcca tcctttttcg cacgatatac aggattttgc
     7921 caaagggttc gtgtagactt tccttggtgt atccaacggc gtcagccggg caggataggt
     7981 gaagtaggcc cacccgcgag cgggtgttcc ttcttcactg tcccttattc gcacctggcg
     8041 gtgctcaacg ggaatcctgc tctgcgaggc tggccggcta ccgccggcgt aacagatgag
     8101 ggcaagcgga tggctgatga aaccaagcca accaggaagg gcagcccacc tatcacggaa
     8161 ttgatccccc tcgaattgga attctagcct ggaataggga agcaccacat cacc
//
