LOCUS       sequence-496428-        5059 bp    DNA     circular SYN 07-JAN-2026
DEFINITION  to generate mRNA for membrane mStayGold(E138D) for zebrafish.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5059)
  AUTHORS   Tschanz A, Matus DQ, Swinburne I
  TITLE     Systematic comparison of mStayGold and mGold2 variants for live 
            imaging in zebrafish.
  JOURNAL   MicroPubl Biol. 2025. 10.17912/micropub.biology.001847.
REFERENCE   2  (bases 1 to 5059)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Jan 7, 2026 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "MicroPubl 
            Biol. 2025. 10.17912/micropub.biology.001847."
COMMENT     Sequence Label: pDQM049
FEATURES             Location/Qualifiers
     source          1..5059
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        1..19
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     1..18
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     regulatory      25..34
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     polyA_signal    918..1039
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(929..948)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     983..1002
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     terminator      1154..1200
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     terminator      1230..1366
                     /label=rrnG terminator
                     /note="transcription terminator from the E. coli ribosomal 
                     RNA rrnG operon (Albrechtsen et al., 1991)"
     misc_feature    1411..1482
                     /label=3' beta-globin insulator
                     /note="insulator downstream of the human beta-globin locus 
                     (Farrell et al., 2002)"
     rep_origin      complement(1708..2296)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(1788..1807)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             join(3042..3110,3111..3902)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(3260..3279)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     terminator      3915..4051
                     /label=rrnG terminator
                     /note="transcription terminator from the E. coli ribosomal 
                     RNA rrnG operon (Albrechtsen et al., 1991)"
     misc_feature    4203..4274
                     /label=5' beta-globin insulator
                     /note="insulator upstream of the human beta-globin locus 
                     (Farrell et al., 2002)"
     terminator      complement(4279..4325)
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     polyA_signal    4343..4391
                     /note="synthetic polyadenylation signal"
     enhancer        4472..4775
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        4777..4979
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     4930..4950
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
ORIGIN
        1 atttaggtga cactatagac tagtgccacc atgggctgca tcaagagcaa gcgtaaggac
       61 aacctgaacg atgatgaagc tgctatggga tgtatcaaat ctaagcgtaa ggataacctt
      121 aatgatgatg agggagcagc tgctcccgct gctgctggat ccatggtaag cacaggagaa
      181 gagctcttca caggtgtggt accctttaaa ttccaactga aagggactat caatggcaag
      241 tctttcaccg ttgagggaga gggagagggt aacagccacg aaggctcgca caagggaaag
      301 tacgtctgca cctccggaaa gctgccaatg tcctgggcag ctctggggac gtcatttgga
      361 tatggaatga agtattacac caagtaccct agcggactca agaactggtt ccacgaagtt
      421 atgcctgaag gttttactta cgatcgtcac atccaatata aaggtgatgg gtctatccac
      481 gccaaacacc agcattttat gaagaatggc acctaccaca acatagtcga gttcacagga
      541 caggatttta aggaaaattc accagtactg acgggagata tgaatgtttc tctccctaac
      601 gacgtccagc atatccctag agatgatggc gtcgaatgcc cggtgaccct tctttaccct
      661 ctgctgtccg acaagtctaa atgtgtggaa gctcaccaga ataccatctg taagcctctg
      721 cataaccagc ctgctccaga cgtcccatat cattggatcc gtaagcagta cacacagtcc
      781 aaggacgata ccgaggagcg tgatcacatc tgccagtcgg aaacactgga ggcgcatctc
      841 taagaattca tacgtatccg gaaccggtgc tagcttgact gactgagata cagcgtacct
      901 tcagctcaca gacatgataa gatacattga tgagtttgga caaaccacaa ctagaatgca
      961 gtgaaaaaaa tgctttattt gtgaaatttg tgatgctatt gctttatttg taaccattat
     1021 aagctgcaat aaacaagtta acaacaacaa ttgcattcat tttatgtttc aggttcaggg
     1081 ggaggtgtgg gaggtttttt aaagcaagta aaacctctac aaatgtggta ttggcccatc
     1141 tctatcggta tcgtagcata accccttggg gcctctaaac gggtcttgag gggttttttg
     1201 tgcccctcgg gccggattgc tatctaccgg cattggcgca gaaaaaaatg cctgatgcga
     1261 cgctgcgcgt cttatactcc cacatatgcc agattcagca acggatacgg cttccccaac
     1321 ttgcccactt ccatacgtgt cctccttacc agaaatttat ccttaaggtc gtcagctatc
     1381 ctgcaggcga tctctcgatt tcgatcaaga cattccttta atggtctttt ctggacacca
     1441 ctaggggtca gaagtagttc atcaaacttt cttccctccc taatctcatt ggttaccttg
     1501 ggctatcgaa acttaattaa ccagtcaagt cagctacttg gcgagatcga cttgtctggg
     1561 tttcgactac gctcagaatt gcgtcagtca agttcgatct ggtccttgct attgcacccg
     1621 ttctccgatt acgagtttca tttaaatcat gtgagcaaaa ggccagcaaa aggccaggaa
     1681 ccgtaaaaag gccgcgttgc tggcgttttt ccataggctc cgcccccctg acgagcatca
     1741 caaaaatcga cgctcaagtc agaggtggcg aaacccgaca ggactataaa gataccaggc
     1801 gtttccccct ggaagctccc tcgtgcgctc tcctgttccg accctgccgc ttaccggata
     1861 cctgtccgcc tttctccctt cgggaagcgt ggcgctttct catagctcac gctgtaggta
     1921 tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt gtgcacgaac cccccgttca
     1981 gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag tccaacccgg taagacacga
     2041 cttatcgcca ctggcagcag ccactggtaa caggattagc agagcgaggt atgtaggcgg
     2101 tgctacagag ttcttgaagt ggtggcctaa ctacggctac actagaagaa cagtatttgg
     2161 tatctgcgct ctgctgaagc cagttacctt cggaaaaaga gttggtagct cttgatccgg
     2221 caaacaaacc accgctggta gcggtggttt ttttgtttgc aagcagcaga ttacgcgcag
     2281 aaaaaaagga tctcaagaag atcctttgat cttttctacg gggtctgacg ctcagtggaa
     2341 cgaaaactca cgttaaggga ttttggtcat gagattatca aaaaggatct tcacctagat
     2401 ccttttaaat taaaaatgaa gttttaaatc aatctaaagt atatatgagt aaacttggtc
     2461 tgacagttac caatgcttaa tcagtgaggc acctatctca gcgatctgtc tatttcgttc
     2521 atccatagtt gcatttaaat ttccgaactc tccaaggccc tcgtcggaaa atcttcaaac
     2581 ctttcgtccg atccatcttg caggctacct ctcgaacgaa ctatcgcaag tctcttggcc
     2641 ggccttgcgc cttggctatt gcttggcagc gcctatcgcc aggtattact ccaatcccga
     2701 atatccgaga tcgggatcac ccgagagaag ttcaacctac atcctcaatc ccgatctatc
     2761 cgagatccga ggaatatcga aatcggggcg cgcctggtgt accgagaacg atcctctcag
     2821 tgcgagtctc gacgatccat atcgttgctt ggcagtcagc cagtcggaat ccagcttggg
     2881 acccaggaag tccaatcgtc agatattgta ctcaagcctg gtcacggcag cgtaccgatc
     2941 tgtttaaacc tagatattga tagtctgatc ggtcaacgta taatcgagtc ctagcttttg
     3001 caaacatcta tcaagagaca ggatcagcag gaggctttcg catgagtatt caacatttcc
     3061 gtgtcgccct tattcccttt tttgcggcat tttgccttcc tgtttttgct cacccagaaa
     3121 cgctggtgaa agtaaaagat gctgaagatc agttgggtgc gcgagtgggt tacatcgaac
     3181 tggatctcaa cagcggtaag atccttgaga gttttcgccc cgaagaacgc tttccaatga
     3241 tgagcacttt taaagttctg ctatgtggcg cggtattatc ccgtattgac gccgggcaag
     3301 agcaactcgg tcgccgcata cactattctc agaatgactt ggttgagtat tcaccagtca
     3361 cagaaaagca tcttacggat ggcatgacag taagagaatt atgcagtgct gccataacca
     3421 tgagtgataa cactgcggcc aacttacttc tgacaacgat tggaggaccg aaggagctaa
     3481 ccgctttttt gcacaacatg ggggatcatg taactcgcct tgatcgttgg gaaccggagc
     3541 tgaatgaagc cataccaaac gacgagcgtg acaccacgat gcctgtagca atggcaacaa
     3601 ccttgcgtaa actattaact ggcgaactac ttactctagc ttcccggcaa cagttgatag
     3661 actggatgga ggcggataaa gttgcaggac cacttctgcg ctcggccctt ccggctggct
     3721 ggtttattgc tgataaatct ggagccggtg agcgtgggtc tcgcggtatc attgcagcac
     3781 tggggccaga tggtaagccc tcccgtatcg tagttatcta cacgacgggg agtcaggcaa
     3841 ctatggatga acgaaataga cagatcgctg agataggtgc ctcactgatt aagcattggt
     3901 aaccgattct aggtgcattg gcgcagaaaa aaatgcctga tgcgacgctg cgcgtcttat
     3961 actcccacat atgccagatt cagcaacgga tacggcttcc ccaacttgcc cacttccata
     4021 cgtgtcctcc ttaccagaaa tttatcctta agatcccgaa tcgtttaaac tcgactctgg
     4081 ctctatcgaa tctccgtcgt ttcgagctta cgcgaacagc cgtggcgctc atttgctcgt
     4141 cgggcatcga atctcgtcag ctatcgtcag cttacctttt tggcagcgat cgcggctccc
     4201 gacatcttgg accattagct ccacaggtat cttcttccct ctagtggtca taacagcagc
     4261 ttcagctacc tctcaattca aaaaacccct caagacccgt ttagaggccc caaggggtta
     4321 tgctatcaat cgttgcgtta cacacacaaa aaaccaacac acatccatct tcgatggata
     4381 gcgattttat tatctaactg ctgatcgagt gtagccagat ctagtaatca attacggggt
     4441 cattagttca tagcccatat atggagttcc gcgttacata acttacggta aatggcccgc
     4501 ctggctgacc gcccaacgac ccccgcccat tgacgtcaat aatgacgtat gttcccatag
     4561 taacgccaat agggactttc cattgacgtc aatgggtgga gtatttacgg taaactgccc
     4621 acttggcagt acatcaagtg tatcatatgc caagtacgcc ccctattgac gtcaatgacg
     4681 gtaaatggcc cgcctggcat tatgcccagt acatgacctt atgggacttt cctacttggc
     4741 agtacatcta cgtattagtc atcgctatta ccatgctgat gcggttttgg cagtacatca
     4801 atgggcgtgg atagcggttt gactcacggg gatttccaag tctccacccc attgacgtca
     4861 atgggagttt gttttggcac caaaatcaac gggactttcc aaaatgtcgt aacaactccg
     4921 ccccattgac gcaaatgggc ggtaggcgtg tacggtggga ggtctatata agcagagctg
     4981 gtttagtgaa ccgtcagatc agatctttgt cgatcctacc atccactcga cacacccgcc
     5041 agcggccgct gccaagctt
//
