LOCUS       sequence-496429-       10412 bp    DNA     circular SYN 07-JAN-2026
DEFINITION  To express the EPEC DarT2(DLAA)-nScCas9(D10A) fusion protein in 
            experiments for Append Editing.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10412)
  AUTHORS   Gupta D, Patinios C, Bassett HV, Kibe A, Collins SP, Kamm C, Wang Y,
            Zhao C, Vollen K, Toussaint C, Calvin I, Cullot G, Aird EJ, Polkoff 
            KM, Nguyen-Vo TP, Migur A, Schut F, Al'Abri IS, Achmedov T, Del Re 
            A, Corn JE, Saliba AE, Crook N, Stepanova AN, Alonso JM, Beisel CL
  TITLE     Targeted DNA ADP-ribosylation triggers templated repair in bacteria 
            and base mutagenesis in eukaryotes.
  JOURNAL   Nat Biotechnol. 2025 Sep 4. doi: 10.1038/s41587-025-02802-w.
  PUBMED    40908325
REFERENCE   2  (bases 1 to 10412)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Jan 7, 2026 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Biotechnol. 2025 Sep 4. doi: 10.1038/s41587-025-02802-w."
COMMENT     Sequence Label: pCBS-5738
FEATURES             Location/Qualifiers
     source          1..10412
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(join(255..1046,1047..1115))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     878..897
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(1116..1220)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      1323..1911
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     1812..1831
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     promoter        2297..2738
                     /gene="S. cerevisiae GAL1"
                     /label=GAL1 promoter
                     /note="inducible promoter, regulated by Gal4"
     protein_bind    2297..2414
                     /label=UAS
                     /bound_moiety="Gal4"
                     /note="upstream activating sequence mediating 
                     Gal4-dependent induction"
     primer_bind     2709..2732
                     /label=GAL1
                     /note="S. cerevisiae GAL1 promoter, forward primer"
     CDS             2792..2812
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             7694..7714
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     promoter        8372..8779
                     /gene="S. cerevisiae LEU2"
                     /label=LEU2 promoter
     CDS             8780..9874
                     /codon_start=1
                     /gene="S. cerevisiae LEU2"
                     /product="3-isopropylmalate dehydrogenase, required for 
                     leucine biosynthesis"
                     /label=LEU2
                     /note="yeast auxotrophic marker"
                     /translation="MSAPKKIVVLPGDHVGQEITAEAIKVLKAISDVRSNVKFDFENHL
                     IGGAAIDATGVPLPDEALEASKKADAVLLGAVGGPKWGTGSVRPEQGLLKIRKELQLYA
                     NLRPCNFASDSLLDLSPIKPQFAKGTDFVVVRELVGGIYFGKRKEDDGDGVAWDSEQYT
                     VPEVQRITRMAAFMALQHEPPLPIWSLDKANVLASSRLWRKTVEETIKNEFPTLKVQHQ
                     LIDSAAMILVKNPTHLNGIIITSNMFGDIISDEASVIPGSLGLLPSASLASLPDKNTAF
                     GLYEPCHGSAPDLPKNKVNPIATILSAAMMLKLSLNLPEEGKAIEDAVKKVLDAGIRTG
                     DLGGSNSTTEVGDAVAEEVKKILA"
     misc_feature    join(10053..10412,1..144)
                     /label=CEN/ARS
                     /note="S. cerevisiae CEN6 centromere fused to an 
                     autonomously replicating sequence"
ORIGIN
        1 cttcattccg taactcttct accttcttta tttactttct aaaatccaaa tacaaaacat
       61 aaaaataaat aaacacagag taaattccca aattattcca tcattaaaag atacgaggcg
      121 cgtgtaagtt acaggcaagc gatccgtccc gagcggccgc gattatcaaa aaggatcttc
      181 acctagatcc ttttaaatta aaaatgaagt tttaaatcaa tctaaagtat atatgagtaa
      241 acttggtctg acagttacca atgcttaatc agtgaggcac ctatctcagc gatctgtcta
      301 tttcgttcat ccatagttgc ctgactcccc gtcgtgtaga taactacgat acgggagggc
      361 ttaccatctg gccccagtgc tgcaatgata ccgcgggacc cacgctcacc ggctccagat
      421 ttatcagcaa taaaccagcc agccggaagg gccgagcgca gaagtggtcc tgcaacttta
      481 tccgcctcca tccagtctat taattgttgc cgggaagcta gagtaagtag ttcgccagtt
      541 aatagtttgc gcaacgttgt tgccattgct acaggcatcg tggtgtcacg ctcgtcgttt
      601 ggtatggctt cattcagctc cggttcccaa cgatcaaggc gagttacatg atcccccatg
      661 ttgtgcaaaa aagcggttag ctccttcggt cctccgatcg ttgtcagaag taagttggcc
      721 gcagtgttat cactcatggt tatggcagca ctgcataatt ctcttactgt catgccatcc
      781 gtaagatgct tttctgtgac tggtgagtac tcaaccaagt cattctgaga atagtgtatg
      841 cggcgaccga gttgctcttg cccggcgtca atacgggata ataccgcgcc acatagcaga
      901 actttaaaag tgctcatcat tggaaaacgt tcttcggggc gaaaactctc aaggatctta
      961 ccgctgttga gatccagttc gatgtaaccc actcgtgcac ccaactgatc ttcagcatct
     1021 tttactttca ccagcgtttc tgggtgagca aaaacaggaa ggcaaaatgc cgcaaaaaag
     1081 ggaataaggg cgacacggaa atgttgaata ctcatactct tcctttttca atattattga
     1141 agcatttatc agggttattg tctcatgagc ggatacatat ttgaatgtat ttagaaaaat
     1201 aaacaaatag gggttccgcg cacatttccc cgaaaagtgc cacctgtcat gaccaaaatc
     1261 ccttaacgtg agttttcgtt ccactgagcg tcagaccccg tagaaaagat caaaggatct
     1321 tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta
     1381 ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc tttttccgaa ggtaactggc
     1441 ttcagcagag cgcagatacc aaatactgtt cttctagtgt agccgtagtt aggccaccac
     1501 ttcaagaact ctgtagcacc gcctacatac ctcgctctgc taatcctgtt accagtggct
     1561 gctgccagtg gcgataagtc gtgtcttacc gggttggact caagacgata gttaccggat
     1621 aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac agcccagctt ggagcgaacg
     1681 acctacaccg aactgagata cctacagcgt gagctatgag aaagcgccac gcttcccgaa
     1741 gggagaaagg cggacaggta tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg
     1801 gagcttccag ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg ccacctctga
     1861 cttgagcgtc gatttttgtg atgctcgtca ggggggcgga gcctatggaa aaacgccagc
     1921 aacgcggcct ttttacggtt cctggccttt tgctggcctt ttgctcacat gttctttcct
     1981 gcgttatccc ctgattctgt ggataaccgt gcggccgccc ctgaattcgc atctagatgg
     2041 tagagccaca aacagccggt acaagcaacg atctccagga ccatctgaat catgcgcgga
     2101 tgacacgaac tcacgacggc gatcacagac attaacccac agtacagaca ctgcgacaac
     2161 gtggcaattc gtcgcaatac cgtctcactg aactggccga taattgcaga cgaacgcccc
     2221 attatcttag cctaaaaaaa ccttctcttt ggaactttca gtaatacgct taactgctca
     2281 ttgctatatt gaagtacgga ttagaagccg ccgagcgggc gacagccctc cgacggatga
     2341 ctctcctccg tgcgtcctcg tcatcaccgg tcgcgttcct gaaacgcaga tgtgcctcgc
     2401 gccgcactgc tccgaacaat aaagattcta caatactagc ttttatggtt atgaagagga
     2461 aaaattggca gtaacctggc cccacaaacc ttcaaattaa cgaatcaaat taacaaccat
     2521 aggatgataa tgcgattagt tttttagcct tatttctggg gtaattaatc agcgaagcga
     2581 tgatttttga tctattaaca gatatataaa tggaaaagct gcataaccac tttaactaat
     2641 actttcaaca ttttcagttt gtattacttc ttattcaaat gtcataaaag tatcaacaaa
     2701 aaattgttaa tatacctcta tactttaacg tcaaggagaa aaaactataa gatctatgaa
     2761 acggacagcc gacggaagcg agttcgagtc accaaagaag aagcggaaag tcgcctatga
     2821 ttactcagcc agcttgaatc cacagaaagc tcttatctgg cgcattgtcc atcgtgataa
     2881 cattccttgg atcttagaca atggtctgca ctgcggtaat agcttggttc aagccgagaa
     2941 ctggattaac atcgacaatc ccgagttgat tggtaaacgc gctgggcacc cggttcccgt
     3001 aggcaccggg ggtacgttgc acgattatgt cccgttctac tttacgcctt ttagtcctat
     3061 gcttctgaat atccattcag gcgccggcgg cattaaacgc cgccccaatg aagagattgt
     3121 aatcttagtt agcaacttac gcaacgtcgc agcccatgac gtgccctttg tttttaccga
     3181 ctcacacgcc tattataatt ggacaaacta ctatacgtcg ttaaactcat tggaccagat
     3241 cgattggcca attttgcaag cccgcgattt ccgccgtgac ccagatgacc cggctaagtt
     3301 tgaacgctac caagcagagg ccttaatctg gcaacactgt ccgatctcat tacttgacgg
     3361 tatcatctgt tactcggaag aggtcgcctt gcagttggag cagtggttgt ttcaacgtaa
     3421 tttgacaatg tcagtacaca cccgttctgg ctggtatttt tcgagtggcg gatcgtctgg
     3481 tggcagttca ggctcggaaa caccaggcac aagcgagtcc gcgacacccg aatcgagtgg
     3541 agggagctcc gggggttccg agaagaaata ttctatcggt ctggccatcg gcactaactc
     3601 agttggatgg gctgtaatta cagacgatta caaggtccct agcaaaaagt ttaaggtgct
     3661 gggtaatacg aatcgcaaga gtattaagaa gaatctgatg ggtgcattac tgtttgattc
     3721 tggcgaaaca gccgaagcta ctcgtttaaa gcgcaccgcc cgccgtcgtt atacacgccg
     3781 caagaatcgc attcgctatc ttcaagaaat cttcgctaat gaaatggcga agctggacga
     3841 ctctttcttc cagcgtttgg aggaaagttt tcttgtggag gaggacaaaa aaaatgaacg
     3901 tcaccccatt ttcgggaact tagcggacga ggtcgcctat caccgtaact atcccactat
     3961 ttaccacctg cgcaaaaagc ttgcggattc tccagaaaag gctgaccttc gcctgattta
     4021 tctggcattg gcacatatca tcaaatttcg tgggcacttc cttattgaag gaaaattgaa
     4081 cgcagaaaat tctgatgtcg caaaactgtt ctaccagttg atccagacat ataatcaatt
     4141 attcgaggag agtcccctgg atgaaatcga agtggatgca aagggtatcc tgtccgcccg
     4201 tctgtcaaag agtaaacgtc ttgaaaagct gattgcggtg tttcccaatg aaaaaaagaa
     4261 cggtttgttc gggaacatta tcgctctggc ccttggttta acccctaact ttaagtcaaa
     4321 ctttgacctg actgaggacg cgaaattaca gctttctaaa gatacttatg acgatgactt
     4381 agatgagctt ctggggcaaa ttggggacca gtacgccgat cttttcagtg cagccaagaa
     4441 tcttagtgac gcgattttgc tttcagacat cttacgttcc aacagcgaag taacaaaggc
     4501 gccactgagc gcctctatgg ttaagcgtta tgacgagcac catcaggacc ttgcacttct
     4561 taagactctg gtgcgtcaac agtttccaga aaagtacgca gagatcttca aggacgatac
     4621 aaaaaacgga tacgccgggt atgtcggaat tggaattaaa catcgcaagc gcacaactaa
     4681 actggcgaca caagaagagt tctacaaatt cattaaacct attcttgaga aaatggacgg
     4741 ggccgaggag ctgttggcca aactgaaccg tgacgacctt ttacgtaaac agcgcacctt
     4801 tgacaacggt tccattcctc accagatcca tcttaaagaa ttgcacgcaa ttttgcgtcg
     4861 tcaggaggag ttttatccat ttctgaaaga gaatcgtgag aaaattgaga aaatccttac
     4921 gttccgcatc ccctactatg ttggtcctct ggctcgcggc aactcccgct tcgcgtggtt
     4981 gacacgtaag tctgaggagg ctatcactcc ctggaacttc gaggaagtgg tggacaaagg
     5041 agccagcgca cagagcttta tcgaacgtat gacaaatttc gacgaacagc ttccaaacaa
     5101 aaaagtctta ccgaagcata gtcttttata tgagtatttc accgtctata atgagttaac
     5161 gaaagtgaag tacgttactg agcgcatgcg taagcccgag tttttaagtg gcgagcaaaa
     5221 aaaggctatt gtcgatcttc tgtttaagac taaccgcaaa gtcaccgtga aacaacttaa
     5281 agaagattat tttaagaaga tcgaatgttt cgattcggta gaaattattg gcgtagagga
     5341 tcgtttcaac gctagtttgg ggacatacca tgacttactt aagatcatca aggacaagga
     5401 ttttttggat aatgaagaaa acgaggatat tctggaggac attgtattga ccctgacact
     5461 gttcgaggac cgtgaaatga ttgaagagcg tttaaaaaca tatgcgcacc tgtttgacga
     5521 taaagtgatg aagcaattaa agcgccgtca ttacactggg tggggtcgtc tttctcgtaa
     5581 aatgattaac ggtatccgtg ataaacagtc tggtaagacg attctggact tccttaagag
     5641 cgatggcttc tcgaaccgta actttatgca actgattcac gatgactccc tgacgtttaa
     5701 agaagaaatt gagaaggctc aagtctctgg gcaaggggac agtctgcacg agcaaattgc
     5761 tgaccttgcc ggaagtcccg cgattaagaa aggaatcttg caaacagtaa aaattgtgga
     5821 tgaattggtt aaagtgatgg gacataaacc cgaaaatatt gtcatcgaga tggcgcgcga
     5881 gaaccagacc actaccaagg ggcttcaaca atcgcgtgaa cgtaagaaac gcatcgaaga
     5941 aggcattaag gagttggaga gccaaattct gaaagaaaat ccagtagaga atactcagtt
     6001 acagaatgaa aaattgtatc tttattactt acagaacgga cgcgatatgt acgtggatca
     6061 agagctggac atcaatcgcc tgagtgacta cgacgtagat catatcgttc ctcagtcgtt
     6121 tatcaaggat gacagcatcg acaataaagt gctgacgcgt agtgtcgaaa atcgcggaaa
     6181 atcggataat gtgccatcgg aggaagtcgt gaaaaaaatg aagaattatt ggcgccaact
     6241 tttgaacgcc aagttgatta cccaacgtaa attcgataat cttaccaagg ctgaacgcgg
     6301 gggcctgtcc gaagcagaca aagcagggtt tattaagcgt caacttgtag agacacgtca
     6361 gattacaaag catgtcgccc gcattcttga ttcgcgcatg aatactaagc gtgataaaaa
     6421 tgacaagccc attcgtgagg ttaaagtaat tacactgaag tctaagctgg ttagtgactt
     6481 tcgtaaagac ttccagttat acaaggtgcg cgatatcaat aattatcatc acgcccatga
     6541 cgcttatctg aatgcggttg taggtacggc cctgatcaag aagtatccaa aattagagtc
     6601 cgagttcgtg tacggtgatt acaaggtcta cgatgtccgt aaaatgattg ccaaatccga
     6661 acaagaaatc ggtaaagcga ctgcaaaacg cttcttctat tctaacatta tgaatttctt
     6721 taagaccgag gtaaaattgg cgaacgggga aattcgtaaa cgtccgttga tcgagacaaa
     6781 cggagaaacg ggcgaggttg tttggaacaa agaaaaagac ttcgccactg ttcgtaaagt
     6841 tctggcgatg ccgcaggtaa acatcgtaaa gaaaaccgaa gttcaaactg gaggattttc
     6901 taaagaatca atcctttcta aacgtgaatc ggcgaagttg attccgcgca aaaaagggtg
     6961 ggacactcgt aaatacggcg gttttgggag tccgactgta gcctatagta tcctggtggt
     7021 tgcaaaggtc gagaagggaa aagctaaaaa actgaaatcc gttaaggtct tggttgggat
     7081 cacgattatg gagaaaggaa gctatgaaaa ggaccctatt ggttttctgg aagccaaagg
     7141 ctacaaggat atcaaaaagg agttgatctt caaattacct aaatattcat tgttcgaact
     7201 ggagaacgga cgccgtcgta tgttagcttc tgcaaccgag ttgcagaagg ctaacgaact
     7261 ggtattgcca caacatctgg tgcgtttact ttattataca cagaacatct cggctacgac
     7321 aggctctaac aatttggggt acatcgagca acatcgtgaa gaattcaagg agattttcga
     7381 aaaaattatt gatttctcgg agaagtatat cttgaaaaat aaagtgaatt caaacttgaa
     7441 atcgagtttt gacgagcaat tcgcggtatc ggattccatt ctgttgtcca acagctttgt
     7501 tagcctgttg aaatatacct cttttggcgc gtcgggagga ttcaccttcc tggaccttga
     7561 cgttaagcaa gggcgcttgc gctaccagac ggttacggag gttctggatg cgaccttgat
     7621 ctatcaaagc atcaccggac tgtacgagac acgtacagat ctgagccaac tgggaggaga
     7681 ctcgcgggct gacccgaaaa agaagagaaa ggtctagatc ctaactcgag ataaagcaat
     7741 cttgatgagg ataatgattt ttttttgaat atacataaat actaccgttt ttctgctaga
     7801 ttttgtgatg acgtaaataa gtacatatta ctttttaagc caagacaaga ttaagcatta
     7861 actttaccct tttctttcta agtttcaata ttagttatca ctgtttaaaa gttatggcga
     7921 gaacgtcggc ggttaaaata tattaccctg aacggctgcc aatgagacga cggggtcatc
     7981 acggctcatc atgcgccaaa caaatgtgtg caatacacgc tcggatgact gcatgatgac
     8041 cgcactgact ggggacagca gatccaccta agcctgtgag agaagcagac acccgacaga
     8101 tcaaggcagt taactagtgc actgcagtac atcgaggaga acttctagta tatctacata
     8161 cctaatatta ttgccttatt aaaaatggaa tcccaacaat tacatcaaaa tccacattct
     8221 cttcaaaatc aattgtcctg tacttccttg ttcatgtgtg ttcaaaaacg ttatatttat
     8281 aggataatta tactctattt ctcaacaagt aattggttgt ttggccgagc ggtctaaggc
     8341 gcctgattca agaaatatct tgaccgcagt taactgtggg aatactcagg tatcgtaaga
     8401 tgcaagagtt cgaatctctt agcaaccatt atttttttcc tcaacataac gagaacacac
     8461 aggggcgcta tcgcacagaa tcaaattcga tgactggaaa ttttttgtta atttcagagg
     8521 tcgcctgacg catatacctt tttcaactga aaaattggga gaaaaaggaa aggtgagagc
     8581 gccggaaccg gcttttcata tagaatagag aagcgttcat gactaaatgc ttgcatcaca
     8641 atacttgaag ttgacaatat tatttaagga cctattgttt tttccaatag gtggttagca
     8701 atcgtcttac tttctaactt ttcttacctt ttacatttca gcaatatata tatatatatt
     8761 tcaaggatat accattctaa tgtctgcccc taagaagatc gtcgttttgc caggtgacca
     8821 cgttggtcaa gaaatcacag ccgaagccat taaggttctt aaagctattt ctgatgttcg
     8881 ttccaatgtc aagttcgatt tcgaaaatca tttaattggt ggtgctgcta tcgatgctac
     8941 aggtgttcca cttccagatg aggcgctgga agcctccaag aaggctgatg ccgttttgtt
     9001 aggtgctgtg ggtggtccta aatggggaac cggtagtgtt agacctgaac aaggtttact
     9061 aaaaatccgt aaagaacttc aattgtacgc caacttaaga ccatgtaact ttgcatccga
     9121 ctctctttta gacttatctc caatcaagcc acaatttgct aaaggtactg acttcgttgt
     9181 tgtcagagaa ttagtgggag gtatttactt tggtaagaga aaggaggacg atggtgatgg
     9241 tgtcgcttgg gatagtgaac aatacaccgt tccagaagtg caaagaatca caagaatggc
     9301 cgctttcatg gccctacaac atgagccacc attgcctatt tggtccttgg ataaagctaa
     9361 tgttttggcc tcttcaagat tatggagaaa aactgtggag gaaaccatca agaacgaatt
     9421 tcctacattg aaggttcaac atcaattgat tgattctgcc gccatgatcc tagttaagaa
     9481 cccaacccac ctaaatggta ttataatcac cagcaacatg tttggtgata tcatctccga
     9541 tgaagcctcc gttatcccag gttccttggg tttgttgcca tctgcgtcct tggcctcttt
     9601 gccagacaag aacaccgcat ttggtttgta cgaaccatgc cacggttctg ctccagattt
     9661 gccaaagaat aaggtcaacc ctatcgccac tatcttgtct gctgcaatga tgttgaaatt
     9721 gtcattgaac ttgcctgaag aaggtaaggc cattgaagat gcagttaaaa aggttttgga
     9781 tgcaggtatc agaactggtg atttaggtgg ttccaacagt accaccgaag tcggtgatgc
     9841 tgtcgccgaa gaagttaaga aaatccttgc ttaacctgga ggacccttct ctttagacta
     9901 ttctactctt atgcacgtaa aaaattctag gaaatatgta ttaactagga gtaaaataac
     9961 cggctagtgg cattcatata gccgtctgtt tacatctaca tcacacattt cgagtgtata
    10021 tctcgcaacg ttggcgttaa ataggcagga gtatcacgtg ctataaaaat aattataatt
    10081 taaatttttt aatataaata tataaattaa aaatagaaag taaaaaaaga aattaaagaa
    10141 aaaatagttt ttgttttccg aagatgtaaa agactctagg gggatcgcca acaaatacta
    10201 ccttttatct tgctcttcct gctctcaggt attaatgccg aattgtttca tcttgtctgt
    10261 gtagaaaacc acacacgaaa atcctgtgat tttacatttt acttatcgtt aatcgaatgt
    10321 atatctattt aatctgcttt tcttgtctaa taaatatata tgtaaagtac gctttttgtt
    10381 gaaatttttt aaacctttgt ttattttttt tt
//
