LOCUS       sequence-496436-       12268 bp    DNA     circular SYN 07-JAN-2026
DEFINITION  Overexpression of EnAsCas12a-BFP with HBB IVS2 intron in 5' UTR with
            blasticidin resistance gene. Contains Cp36 recombination site..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12268)
  AUTHORS   Arana S, Du PP, Vaughan-Jackson A, Enright N, Spees K, Valbuena R, 
            Garcia CA, Nguyen T, Venida A, Seczynska M, Bintu L, Lehner PJ, 
            Prolo LM, Bassik MC
  TITLE     Reduced Cas9 transgene silencing by incorporation of intron 
            sequences.
  JOURNAL   Nat Commun. 2025 Nov 27;16(1):10656. doi: 
            10.1038/s41467-025-65669-0.
  PUBMED    41309578
REFERENCE   2  (bases 1 to 12268)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Jan 7, 2026 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2025-11-27"; volume: "16(1):10656. doi"; pages: " 
            10.1038/s41467-025-65669-0"
COMMENT     Sequence Label: pPD685 HBB_IVS2-EnAsCas12a-BFP
FEATURES             Location/Qualifiers
     source          1..12268
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        500..1709
                     /label=UbC promoter
                     /note="human ubiquitin C promoter"
     primer_bind     1546..1565
                     /label=hUBCpro-F
                     /note="Human Ubiquitin C (UbC) promoter, forward primer"
     CDS             1713..2111
                     /codon_start=1
                     /gene="Aspergillus terreus BSD"
                     /product="blasticidin S deaminase"
                     /label=BSD
                     /note="confers resistance to blasticidin"
                     /translation="MAKPLSQEESTLIERATATINSIPISEDYSVASAALSSDGRIFTG
                     VNVYHFTGGPCAELVVLGTAAAAAAGNLTCIVAIGNENRGILSPCGRCRQVLLDLHPGI
                     KAIVKDSDGQPTAVGIRELLPSGYVWEG"
     polyA_signal    2138..2186
                     /note="synthetic polyadenylation signal"
     CDS             join(2452..2520,2521..3312)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(2670..2689)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     terminator      3325..3461
                     /label=rrnG terminator
                     /note="transcription terminator from the E. coli ribosomal 
                     RNA rrnG operon (Albrechtsen et al., 1991)"
     misc_feature    3613..3684
                     /label=5' beta-globin insulator
                     /note="insulator upstream of the human beta-globin locus 
                     (Farrell et al., 2002)"
     terminator      complement(3689..3735)
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     polyA_signal    3753..3801
                     /note="synthetic polyadenylation signal"
     promoter        3942..5120
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          4173..5111
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     5068..5088
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     regulatory      6102..6111
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             6114..6134
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             6159..10076
                     /codon_start=1
                     /product="CRISPR-associated protein from Acidaminococcus 
                     sp. BV3L6 (Zetsche et al., 2015)"
                     /label=AsCpf1
                     /translation="TQFEGFTNLYQVSKTLRFELIPQGKTLKHIQEQGFIEEDKARNDH
                     YKELKPIIDRIYKTYADQCLQLVQLDWENLSAAIDSYRKEKTEETRNALIEEQATYRNA
                     IHDYFIGRTDNLTDAINKRHAEIYKGLFKAELFNGKVLKQLGTVTTTEHENALLRSFDK
                     FTTYFSGFYRNRKNVFSAEDISTAIPHRIVQDNFPKFKENCHIFTRLITAVPSLREHFE
                     NVKKAIGIFVSTSIEEVFSFPFYNQLLTQTQIDLYNQLLGGISREAGTEKIKGLNEVLN
                     LAIQKNDETAHIIASLPHRFIPLFKQILSDRNTLSFILEEFKSDEEVIQSFCKYKTLLR
                     NENVLETAEALFNELNSIDLTHIFISHKKLETISSALCDHWDTLRNALYERRISELTGK
                     ITKSAKEKVQRSLKHEDINLQEIISAAGKELSEAFKQKTSEILSHAHAALDQPLPTTLK
                     KQEEKEILKSQLDSLLGLYHLLDWFAVDESNEVDPEFSARLTGIKLEMEPSLSFYNKAR
                     NYATKKPYSVEKFKLNFQMPTLARGWDVNREKNNGAILFVKNGLYYLGIMPKQKGRYKA
                     LSFEPTEKTSEGFDKMYYDYFPDAAKMIPKCSTQLKAVTAHFQTHTTPILLSNNFIEPL
                     EITKEIYDLNNPEKEPKKFQTAYAKKTGDQKGYREALCKWIDFTRDFLSKYTKTTSIDL
                     SSLRPSSQYKDLGEYYAELNPLLYHISFQRIAEKEIMDAVETGKLYLFQIYNKDFAKGH
                     HGKPNLHTLYWTGLFSPENLAKTSIKLNGQAELFYRPKSRMKRMAHRLGEKMLNKKLKD
                     QKTPIPDTLYQELYDYVNHRLSHDLSDEARALLPNVITKEVSHEIIKDRRFTSDKFFFH
                     VPITLNYQAANSPSKFNQRVNAYLKEHPETPIIGIDRGERNLIYITVIDSTGKILEQRS
                     LNTIQQFDYQKKLDNREKERVAARQAWSVVGTIKDLKQGYLSQVIHEIVDLMIHYQAVV
                     VLENLNFGFKSKRTGIAEKAVYQQFEKMLIDKLNCLVLKDYPAEKVGGVLNPYQLTDQF
                     TSFAKMGTQSGFLFYVPAPYTSKIDPLTGFVDPFVWKTIKNHESRKHFLEGFDFLHYDV
                     KTGDFILHFKMNRNLSFQRGLPGFMPAWDIVFEKNETQFDAKGTPFIAGKRIVPVIENH
                     RFTGRYRDLYPANELIALLEEKGIVFRDGSNILPKLLENDDSHAIDTMVALIRSVLQMR
                     NSNAATGEDYINSPVRDLNGVCFDSRFQNPEWPMDADANGAYHIALKGQLLLNHLKESK
                     DLKLQNGISNQDWLAYIQELRN"
     regulatory      9796..9805
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             10077..10124
                     /codon_start=1
                     /product="bipartite nuclear localization signal from 
                     nucleoplasmin"
                     /label=nucleoplasmin NLS
                     /translation="KRPAATKKAGQAKKKK"
     CDS             10140..10838
                     /codon_start=1
                     /product="monomeric blue fluorescent protein"
                     /label=TagBFP
                     /note="mammalian codon-optimized"
                     /translation="SELIKENMHMKLYMEGTVDNHHFKCTSEGEGKPYEGTQTMRIKVV
                     EGGPLPFAFDILATSFLYGSKTFINHTQGIPDFFKQSFPEGFTWERVTTYEDGGVLTAT
                     QDTSLQDGCLIYNVKIRGVNFTSNGPVMQKKTLGWEAFTETLYPADGGLEGRNDMALKL
                     VGGSHLIANIKTTYRSKKPAKNLKMPGVYYVDYRLERIKEANNETYVEQHEVAVARYCD
                     LPSKLGHKLN"
     polyA_signal    10890..11011
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(10901..10920)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     10955..10974
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     terminator      11126..11172
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     terminator      11202..11338
                     /label=rrnG terminator
                     /note="transcription terminator from the E. coli ribosomal 
                     RNA rrnG operon (Albrechtsen et al., 1991)"
     misc_feature    11383..11454
                     /label=3' beta-globin insulator
                     /note="insulator downstream of the human beta-globin locus 
                     (Farrell et al., 2002)"
     rep_origin      complement(11680..12268)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(11760..11779)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 gaagatcctt tgatcttttc tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa
       61 gggattttgg tcatgagatt atcaaaaagg atcttcacct agatcctttt aaattaaaaa
      121 tgaagtttta aatcaatcta aagtatatat gagtaaactt ggtctgacag ttaccaatgc
      181 ttaatcagtg aggcacctat ctcagcgatc tgtctatttc gttcatccat agttgcattt
      241 aaatttccga actctccaag gccctcgtcg gaaaatcttc aaacctttcg tccgatccat
      301 cttgcaggct acctctcgaa cgaactatcg caagtctctt ggccggcctt gcgccttggc
      361 tattgcttgg cagcgcctat cgccaggtat tactccaatc ccgaatatcc gagatcggga
      421 tcacccgaga gaagttcaac ctacatcctc aatcccgatc tatccgagat ccgaggaata
      481 tcgaaatcgg ggcgcgcctg gcctccgcgc cgggttttgg cgcctcccgc gggcgccccc
      541 ctcgtcacgg cgagcgctgc cacgtcagac gaagggcgca ggagcgtcct gatccttccg
      601 cccggacgct caggacagcg gcccgctgct cataagactc ggccttagaa ccccagtatc
      661 agcagaagga cattttagga cgggacttgg gtgactctag ggcactggtt ttctttccag
      721 agagcggaac aggcgaggaa aagtagtccc ttctcggcga ttctgcggag ggatctccgt
      781 ggggcggtga acgccgatga ttatataagg acgcgccggg tgtggcacag ctagttccgt
      841 cgcagccggg atttgggtcg cggttcttgt ttgtggatcg ctgtgatcgt cacttggtga
      901 gtagcgggct gctgggctgg ccggggcttt cgtggccgcc gggccgctcg gtgggacgga
      961 agcgtgtgga gagaccgcca agggctgtag tctgggtccg cgagcaaggt tgccctgaac
     1021 tgggggttgg ggggagcgca gcaaaatggc ggctgttccc gagtcttgaa tggaagacgc
     1081 ttgtgaggcg ggctgtgagg tcgttgaaac aaggtggggg gcatggtggg cggcaagaac
     1141 ccaaggtctt gagcccttcg ctaatgcggg aaagctctta ttcgggtgag atgggctggg
     1201 caccatctgg ggaccctgac gtgaagtttg tcactgactg gagaactcgg tttgtcgtct
     1261 gttgcggggg cggcagttat ggcggtgccg ttgggcagtg cacccgtacc tttgggagcg
     1321 cgcgccctcg tcgtgtcgtg acgtcacccg ttctgttggc ttataatgca gggtggggcc
     1381 acctgccggt aggtgtgcgg taggcttttc tccgtcgcag gacgcagggt tcgggcctag
     1441 ggtaggctct cctgaatcga caggcgccgg acctctggtg aggggaggga taagtgaggc
     1501 gtcagtttct ttggtcggtt ttatgtacct atcttcttaa gtagctgaag ctccggtttt
     1561 gaactatgcg ctcggggttg gcgagtgtgt tttgtgaagt tttttaggca ccttttgaaa
     1621 tgtaatcatt tgggtcaata tgtaattttc agtgttagac ttgtaaattg tccgctaaat
     1681 tctggccgtt tttggctttt ttgttagaca acatggccaa gcctttgtct caagaagaat
     1741 ccaccctcat tgaaagagca acggctacaa tcaacagcat ccccatctct gaagactaca
     1801 gcgtcgccag cgcagctctc tctagcgacg gccgcatctt cactggtgtc aatgtatatc
     1861 attttactgg gggaccttgt gcagaactcg tggtgctggg cactgctgct gctgcggcag
     1921 ctggcaacct gacttgtatc gtcgcgatcg gaaatgagaa caggggcatc ttgagcccct
     1981 gcggacggtg ccgacaggtg cttctcgatc tgcatcctgg gatcaaagcc atagtgaagg
     2041 acagtgatgg acagccgacg gcagttggga ttcgtgaatt gctgccctct ggttatgtgt
     2101 gggagggcta agctagtatg taagcctagt cttagataat aaaatcgcta tccatcgaag
     2161 atggatgtgt gttggttttt tgtgtgtgta acgctaggcg cgcctggtgt accgagaacg
     2221 atcctctcag tgcgagtctc gacgatccat atcgttgctt ggcagtcagc cagtcggaat
     2281 ccagcttggg acccaggaag tccaatcgtc agatattgta ctcaagcctg gtcacggcag
     2341 cgtaccgatc tgtttaaacc tagatattga tagtctgatc ggtcaacgta taatcgagtc
     2401 ctagcttttg caaacatcta tcaagagaca ggatcagcag gaggctttcg catgagtatt
     2461 caacatttcc gtgtcgccct tattcccttt tttgcggcat tttgccttcc tgtttttgct
     2521 cacccagaaa cgctggtgaa agtaaaagat gctgaagatc agttgggtgc gcgagtgggt
     2581 tacatcgaac tggatctcaa cagcggtaag atccttgaga gttttcgccc cgaagaacgc
     2641 tttccaatga tgagcacttt taaagttctg ctatgtggcg cggtattatc ccgtattgac
     2701 gccgggcaag agcaactcgg tcgccgcata cactattctc agaatgactt ggttgagtat
     2761 tcaccagtca cagaaaagca tcttacggat ggcatgacag taagagaatt atgcagtgct
     2821 gccataacca tgagtgataa cactgcggcc aacttacttc tgacaacgat tggaggaccg
     2881 aaggagctaa ccgctttttt gcacaacatg ggggatcatg taactcgcct tgatcgttgg
     2941 gaaccggagc tgaatgaagc cataccaaac gacgagcgtg acaccacgat gcctgtagca
     3001 atggcaacaa ccttgcgtaa actattaact ggcgaactac ttactctagc ttcccggcaa
     3061 cagttgatag actggatgga ggcggataaa gttgcaggac cacttctgcg ctcggccctt
     3121 ccggctggct ggtttattgc tgataaatct ggagccggtg agcgtgggtc tcgcggtatc
     3181 attgcagcac tggggccaga tggtaagccc tcccgtatcg tagttatcta cacgacgggg
     3241 agtcaggcaa ctatggatga acgaaataga cagatcgctg agataggtgc ctcactgatt
     3301 aagcattggt aaccgattct aggtgcattg gcgcagaaaa aaatgcctga tgcgacgctg
     3361 cgcgtcttat actcccacat atgccagatt cagcaacgga tacggcttcc ccaacttgcc
     3421 cacttccata cgtgtcctcc ttaccagaaa tttatcctta agatcccgaa tcgtttaaac
     3481 tcgactctgg ctctatcgaa tctccgtcgt ttcgagctta cgcgaacagc cgtggcgctc
     3541 atttgctcgt cgggcatcga atctcgtcag ctatcgtcag cttacctttt tggcagcgat
     3601 cgcggctccc gacatcttgg accattagct ccacaggtat cttcttccct ctagtggtca
     3661 taacagcagc ttcagctacc tctcaattca aaaaacccct caagacccgt ttagaggccc
     3721 caaggggtta tgctatcaat cgttgcgtta cacacacaaa aaaccaacac acatccatct
     3781 tcgatggata gcgattttat tatctaactg ctgatcgagt gtagccctta accgcttttg
     3841 aatgtccgtc ttagttaatc cctaattgaa cgctccaaaa ggtgacttcc aatagggatt
     3901 tattcctttt aaaattaacg gcataatcgt agatctcgtg aggctccggt gcccgtcagt
     3961 gggcagagcg cacatcgccc acagtccccg agaagttggg gggaggggtc ggcaattgaa
     4021 ccggtgccta gagaaggtgg cgcggggtaa actgggaaag tgatgtcgtg tactggctcc
     4081 gcctttttcc cgagggtggg ggagaaccgt atataagtgc actagtcgcc gtgaacgttc
     4141 tttttcgcaa cgggtttgcc gccagaacac aggtaagtgc cgtgtgtggt tcccgcgggc
     4201 ctggcctctt tacgggttat ggcccttgcg tgccttgaat tacttccacc tggctgcagt
     4261 acgtgattct tgatcccgag cttcgggttg gaagtgggtg ggagagttcg tggccttgcg
     4321 cttaaggagc cccttcgcct cgtgcttgag ttgtggcctg gcctgggcgc tggggccgcc
     4381 gcgtgcgaat ctggtggcac cttcgcgcct gtctcgctgc tttcgataag tctctagcca
     4441 tttaaaattt ttgatgacct gctgcgacgc tttttttctg gcaagatagt cttgtaaatg
     4501 cgggccaaga tcagcacact ggtatttcgg tttttggggc cgcgggcggc gacggggccc
     4561 gtgcgtccca gcgcacatgt tcggcgaggc ggggcctgcg agcgcggcca ccgagaatcg
     4621 gacgggggta gtctcaagct gcccggcctg ctctggtgcc tggcctcgcg ccgccgtgta
     4681 tcgccccgcc ctgggcggca aggctggccc ggtcggcacc agttgcgtga gcggaaagat
     4741 ggccgcttcc cggccctgct gcagggagca caaaatggag gacgcggcgc tcgggagagc
     4801 gggcgggtga gtcacccaca caaaggaaaa gggcctttcc gtcctcagcc gtcgcttcat
     4861 gtgactccac ggagtaccgg gcgccgtcca ggcacctcga ttagttctcc agcttttgga
     4921 gtacgtcgtc tttaggttgg ggggaggggt tttatgcgat ggagtttccc cacactgagt
     4981 gggtggagac tgaagttagg ccagcttggc acttgatgta attctccttg gaatttgccc
     5041 tttttgagtt tggatcttgg ttcattctca agcctcagac agtggttcaa agtttttttc
     5101 ttccatttca ggtgtcgtga aaactacccc taaaagccaa aagatctttg tcgatcctac
     5161 catccactcg acacacccgc cagcggccgc tgccaagctt gctaggaatt cgtgagtcta
     5221 tgggacgctt gatgttttct ttccccttct tttctatggt taagttcatg tcataggaag
     5281 gggataagta acagggtaca gtttagaatg ggaaacagac gaatgattgc atcagtgtgg
     5341 aagtctcagg atcgttttag tttcttttat ttgctgttca taacaattgt tttcttttgt
     5401 ttaattcttg ctttcttttt ttttcttctc cgcaattttt actattatac ttaatgcctt
     5461 aacattgtgt ataacaaaag gaaatatctc tgagatacat taagtaactt aaaaaaaaac
     5521 tttacacagt ctgcctagta cattactatt tggaatatat gtgtgcttat ttgcatattc
     5581 ataatctccc tactttattt tcttttattt ttaattgata cataatcatt atacatattt
     5641 atgggttaaa gtgtaatgtt ttaatatgtg tacacatatt gaccaaatca gggtaatttt
     5701 gcatttgtaa ttttaaaaaa tgctttcttc ttttaatata cttttttgtt tatcttattt
     5761 ctaatacttt ccctaatctc tttctttcag ggcaataatg atacaatgta tcatgcctct
     5821 ttgcaccatt ctaaagaata acagtgataa tttctgggtt aaggcaatag caatatctct
     5881 gcatataaat atttctgcat ataaattgta actgatgtaa gaggtttcat attgctaata
     5941 gcagctacaa tccagctacc attctgcttt tattttatgg ttgggataag gctggattat
     6001 tctgagtcca agctaggccc ttttgctaat catgttcata cctcttatct tcctcccaca
     6061 gcgcgtctgc aggatatcaa gcttgcggta ccgcgggccc ggccaccatg gccccaaaga
     6121 agaagcggaa ggtcggtatc cacggagtcc cagcagccac acagttcgag ggctttacca
     6181 acctgtatca ggtgagcaag acactgcggt ttgagctgat cccacagggc aagaccctga
     6241 agcacatcca ggagcagggc ttcatcgagg aggacaaggc ccgcaatgat cactacaagg
     6301 agctgaagcc catcatcgat cggatctaca agacctatgc cgaccagtgc ctgcagctgg
     6361 tgcagctgga ttgggagaac ctgagcgccg ccatcgactc ctatagaaag gagaaaaccg
     6421 aggagacaag gaacgccctg atcgaggagc aggccacata tcgcaatgcc atccacgact
     6481 acttcatcgg ccggacagac aacctgaccg atgccatcaa taagagacac gccgagatct
     6541 acaagggcct gttcaaggcc gagctgttta atggcaaggt gctgaagcag ctgggcaccg
     6601 tgaccacaac cgagcacgag aacgccctgc tgcggagctt cgacaagttt acaacctact
     6661 tctccggctt ttatcggaac aggaagaacg tgttcagcgc cgaggatatc agcacagcca
     6721 tcccacaccg catcgtgcag gacaacttcc ccaagtttaa ggagaattgt cacatcttca
     6781 cacgcctgat caccgccgtg cccagcctgc gggagcactt tgagaacgtg aagaaggcca
     6841 tcggcatctt cgtgagcacc tccatcgagg aggtgttttc cttccctttt tataaccagc
     6901 tgctgacaca gacccagatc gacctgtata accagctgct gggaggaatc tctcgggagg
     6961 caggcaccga gaagatcaag ggcctgaacg aggtgctgaa tctggccatc cagaagaatg
     7021 atgagacagc ccacatcatc gcctccctgc cacacagatt catccccctg tttaagcaga
     7081 tcctgtccga taggaacacc ctgtctttca tcctggagga gtttaagagc gacgaggaag
     7141 tgatccagtc cttctgcaag tacaagacac tgctgagaaa cgagaacgtg ctggagacag
     7201 ccgaggccct gtttaacgag ctgaacagca tcgacctgac acacatcttc atcagccaca
     7261 agaagctgga gacaatcagc agcgccctgt gcgaccactg ggatacactg aggaatgccc
     7321 tgtatgagcg gagaatctcc gagctgacag gcaagatcac caagtctgcc aaggagaagg
     7381 tgcagcgcag cctgaagcac gaggatatca acctgcagga gatcatctct gccgcaggca
     7441 aggagctgag cgaggccttc aagcagaaaa ccagcgagat cctgtcccac gcacacgccg
     7501 ccctggatca gccactgcct acaaccctga agaagcagga ggagaaggag atcctgaagt
     7561 ctcagctgga cagcctgctg ggcctgtacc acctgctgga ctggtttgcc gtggatgagt
     7621 ccaacgaggt ggaccccgag ttctctgccc ggctgaccgg catcaagctg gagatggagc
     7681 cttctctgag cttctacaac aaggccagaa attatgccac caagaagccc tactccgtgg
     7741 agaagttcaa gctgaacttt cagatgccta cactggcccg cggctgggac gtgaatcggg
     7801 agaagaacaa tggcgccatc ctgtttgtga agaacggcct gtactatctg ggcatcatgc
     7861 caaagcagaa gggcaggtat aaggccctga gcttcgagcc cacagagaaa accagcgagg
     7921 gctttgataa gatgtactat gactacttcc ctgatgccgc caagatgatc ccaaagtgca
     7981 gcacccagct gaaggccgtg acagcccact ttcagaccca cacaaccccc atcctgctgt
     8041 ccaacaattt catcgagcct ctggagatca caaaggagat ctacgacctg aacaatcctg
     8101 agaaggagcc aaagaagttt cagacagcct acgccaagaa aaccggcgac cagaagggct
     8161 acagagaggc cctgtgcaag tggatcgact tcacaaggga ttttctgtcc aagtatacca
     8221 agacaacctc tatcgatctg tctagcctgc ggccatcctc tcagtataag gacctgggcg
     8281 agtactatgc cgagctgaat cccctgctgt accacatcag cttccagaga atcgccgaga
     8341 aggagatcat ggatgccgtg gagacaggca agctgtacct gttccagatc tataacaagg
     8401 actttgccaa gggccaccac ggcaagccta atctgcacac actgtattgg accggcctgt
     8461 tttctccaga gaacctggcc aagacaagca tcaagctgaa tggccaggcc gagctgttct
     8521 accgccctaa gtccaggatg aagaggatgg cacaccggct gggagagaag atgctgaaca
     8581 agaagctgaa ggatcagaaa accccaatcc ccgacaccct gtaccaggag ctgtacgact
     8641 atgtgaatca cagactgtcc cacgacctgt ctgatgaggc cagggccctg ctgcccaacg
     8701 tgatcaccaa ggaggtgtct cacgagatca tcaaggatag gcgctttacc agcgacaagt
     8761 tctttttcca cgtgcctatc acactgaact atcaggccgc caattcccca tctaagttca
     8821 accagagggt gaatgcctac ctgaaggagc accccgagac acctatcatc ggcatcgatc
     8881 ggggcgagag aaacctgatc tatatcacag tgatcgactc caccggcaag atcctggagc
     8941 agcggagcct gaacaccatc cagcagtttg attaccagaa gaagctggac aacagggaga
     9001 aggagagggt ggcagcaagg caggcctggt ctgtggtggg cacaatcaag gatctgaagc
     9061 agggctatct gagccaggtc atccacgaga tcgtggacct gatgatccac taccaggccg
     9121 tggtggtgct ggagaacctg aatttcggct ttaagagcaa gaggaccggc atcgccgaga
     9181 aggccgtgta ccagcagttc gagaagatgc tgatcgataa gctgaattgc ctggtgctga
     9241 aggactatcc agcagagaaa gtgggaggcg tgctgaaccc ataccagctg acagaccagt
     9301 tcacctcctt tgccaagatg ggcacccagt ctggcttcct gttttacgtg cctgccccat
     9361 atacatctaa gatcgatccc ctgaccggct tcgtggaccc cttcgtgtgg aaaaccatca
     9421 agaatcacga gagccgcaag cacttcctgg agggcttcga ctttctgcac tacgacgtga
     9481 aaaccggcga cttcatcctg cactttaaga tgaacagaaa tctgtccttc cagaggggcc
     9541 tgcccggctt tatgcctgca tgggatatcg tgttcgagaa gaacgagaca cagtttgacg
     9601 ccaagggcac ccctttcatc gccggcaaga gaatcgtgcc agtgatcgag aatcacagat
     9661 tcaccggcag ataccgggac ctgtatcctg ccaacgagct gatcgccctg ctggaggaga
     9721 agggcatcgt gttcagggat ggctccaaca tcctgccaaa gctgctggag aatgacgatt
     9781 ctcacgccat cgacaccatg gtggccctga tccgcagcgt gctgcagatg cggaactcca
     9841 atgccgccac aggcgaggac tatatcaaca gccccgtgcg cgatctgaat ggcgtgtgct
     9901 tcgactcccg gtttcagaac ccagagtggc ccatggacgc cgatgccaat ggcgcctacc
     9961 acatcgccct gaagggccag ctgctgctga atcacctgaa ggagagcaag gatctgaagc
    10021 tgcagaacgg catctccaat caggactggc tggcctacat ccaggagctg cgcaacaaaa
    10081 ggccggcggc cacgaaaaag gccggccagg caaaaaagaa aaagggcagc agcggatcca
    10141 gcgagctgat taaggagaac atgcacatga agctgtacat ggagggcacc gtggacaacc
    10201 atcacttcaa gtgcacatcc gagggcgaag gcaagcccta cgagggcacc cagaccatga
    10261 gaatcaaggt ggtcgagggc ggccctctcc ccttcgcctt cgacatcctg gctactagct
    10321 tcctctacgg cagcaagacc ttcatcaacc acacccaggg catccccgac ttcttcaagc
    10381 agtccttccc tgagggcttc acatgggaga gagtcaccac atacgaagac gggggcgtgc
    10441 tgaccgctac ccaggacacc agcctccagg acggctgcct catctacaac gtcaagatca
    10501 gaggggtgaa cttcacatcc aacggccctg tgatgcagaa gaaaacactc ggctgggagg
    10561 ccttcaccga gacgctgtac cccgctgacg gcggcctgga aggcagaaac gacatggccc
    10621 tgaagctcgt gggcgggagc catctgatcg caaacatcaa gaccacatat agatccaaga
    10681 aacccgctaa gaacctcaag atgcctggcg tctactatgt ggactacaga ctggaaagaa
    10741 tcaaggaggc caacaacgag acctacgtcg agcagcacga ggtggcagtg gccagatact
    10801 gcgacctccc tagcaaactg gggcacaagc ttaattagta actagcttga ctgactgaga
    10861 tacagcgtac cttcagctca cagacatgat aagatacatt gatgagtttg gacaaaccac
    10921 aactagaatg cagtgaaaaa aatgctttat ttgtgaaatt tgtgatgcta ttgctttatt
    10981 tgtaaccatt ataagctgca ataaacaagt taacaacaac aattgcattc attttatgtt
    11041 tcaggttcag ggggaggtgt gggaggtttt ttaaagcaag taaaacctct acaaatgtgg
    11101 tattggccca tctctatcgg tatcgtagca taaccccttg gggcctctaa acgggtcttg
    11161 aggggttttt tgtgcccctc gggccggatt gctatctacc ggcattggcg cagaaaaaaa
    11221 tgcctgatgc gacgctgcgc gtcttatact cccacatatg ccagattcag caacggatac
    11281 ggcttcccca acttgcccac ttccatacgt gtcctcctta ccagaaattt atccttaagg
    11341 tcgtcagcta tcctgcaggc gatctctcga tttcgatcaa gacattcctt taatggtctt
    11401 ttctggacac cactaggggt cagaagtagt tcatcaaact ttcttccctc cctaatctca
    11461 ttggttacct tgggctatcg aaacttaatt aaccagtcaa gtcagctact tggcgagatc
    11521 gacttgtctg ggtttcgact acgctcagaa ttgcgtcagt caagttcgat ctggtccttg
    11581 ctattgcacc cgttctccga ttacgagttt catttaaatc atgtgagcaa aaggccagca
    11641 aaaggccagg aaccgtaaaa aggccgcgtt gctggcgttt ttccataggc tccgcccccc
    11701 tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg cgaaacccga caggactata
    11761 aagataccag gcgtttcccc ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc
    11821 gcttaccgga tacctgtccg cctttctccc ttcgggaagc gtggcgcttt ctcatagctc
    11881 acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc aagctgggct gtgtgcacga
    11941 accccccgtt cagcccgacc gctgcgcctt atccggtaac tatcgtcttg agtccaaccc
    12001 ggtaagacac gacttatcgc cactggcagc agccactggt aacaggatta gcagagcgag
    12061 gtatgtaggc ggtgctacag agttcttgaa gtggtggcct aactacggct acactagaag
    12121 aacagtattt ggtatctgcg ctctgctgaa gccagttacc ttcggaaaaa gagttggtag
    12181 ctcttgatcc ggcaaacaaa ccaccgctgg tagcggtggt ttttttgttt gcaagcagca
    12241 gattacgcgc agaaaaaaag gatctcaa
//
