LOCUS       sequence-496440-       12103 bp    DNA     circular SYN 07-JAN-2026
DEFINITION  Overexpression of EnAsCas12a-BFP with blasticidin resistance gene. 
            Contains minimal A2UCOE and Cp36 recombination site..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12103)
  AUTHORS   Arana S, Du PP, Vaughan-Jackson A, Enright N, Spees K, Valbuena R, 
            Garcia CA, Nguyen T, Venida A, Seczynska M, Bintu L, Lehner PJ, 
            Prolo LM, Bassik MC
  TITLE     Reduced Cas9 transgene silencing by incorporation of intron 
            sequences.
  JOURNAL   Nat Commun. 2025 Nov 27;16(1):10656. doi: 
            10.1038/s41467-025-65669-0.
  PUBMED    41309578
REFERENCE   2  (bases 1 to 12103)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Jan 7, 2026 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2025-11-27"; volume: "16(1):10656. doi"; pages: " 
            10.1038/s41467-025-65669-0"
COMMENT     Sequence Label: pPD686 A2UCOE-EnAsCas12a-BFP
FEATURES             Location/Qualifiers
     source          1..12103
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        500..1709
                     /label=UbC promoter
                     /note="human ubiquitin C promoter"
     primer_bind     1546..1565
                     /label=hUBCpro-F
                     /note="Human Ubiquitin C (UbC) promoter, forward primer"
     CDS             1713..2111
                     /codon_start=1
                     /gene="Aspergillus terreus BSD"
                     /product="blasticidin S deaminase"
                     /label=BSD
                     /note="confers resistance to blasticidin"
                     /translation="MAKPLSQEESTLIERATATINSIPISEDYSVASAALSSDGRIFTG
                     VNVYHFTGGPCAELVVLGTAAAAAAGNLTCIVAIGNENRGILSPCGRCRQVLLDLHPGI
                     KAIVKDSDGQPTAVGIRELLPSGYVWEG"
     polyA_signal    2138..2186
                     /note="synthetic polyadenylation signal"
     CDS             join(2452..2520,2521..3312)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(2670..2689)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     terminator      3325..3461
                     /label=rrnG terminator
                     /note="transcription terminator from the E. coli ribosomal 
                     RNA rrnG operon (Albrechtsen et al., 1991)"
     misc_feature    3613..3684
                     /label=5' beta-globin insulator
                     /note="insulator upstream of the human beta-globin locus 
                     (Farrell et al., 2002)"
     terminator      complement(3689..3735)
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     polyA_signal    3753..3801
                     /note="synthetic polyadenylation signal"
     promoter        4627..5805
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          4858..5796
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     5753..5773
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     regulatory      5937..5946
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             5949..5969
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             5994..9911
                     /codon_start=1
                     /product="CRISPR-associated protein from Acidaminococcus 
                     sp. BV3L6 (Zetsche et al., 2015)"
                     /label=AsCpf1
                     /translation="TQFEGFTNLYQVSKTLRFELIPQGKTLKHIQEQGFIEEDKARNDH
                     YKELKPIIDRIYKTYADQCLQLVQLDWENLSAAIDSYRKEKTEETRNALIEEQATYRNA
                     IHDYFIGRTDNLTDAINKRHAEIYKGLFKAELFNGKVLKQLGTVTTTEHENALLRSFDK
                     FTTYFSGFYRNRKNVFSAEDISTAIPHRIVQDNFPKFKENCHIFTRLITAVPSLREHFE
                     NVKKAIGIFVSTSIEEVFSFPFYNQLLTQTQIDLYNQLLGGISREAGTEKIKGLNEVLN
                     LAIQKNDETAHIIASLPHRFIPLFKQILSDRNTLSFILEEFKSDEEVIQSFCKYKTLLR
                     NENVLETAEALFNELNSIDLTHIFISHKKLETISSALCDHWDTLRNALYERRISELTGK
                     ITKSAKEKVQRSLKHEDINLQEIISAAGKELSEAFKQKTSEILSHAHAALDQPLPTTLK
                     KQEEKEILKSQLDSLLGLYHLLDWFAVDESNEVDPEFSARLTGIKLEMEPSLSFYNKAR
                     NYATKKPYSVEKFKLNFQMPTLARGWDVNREKNNGAILFVKNGLYYLGIMPKQKGRYKA
                     LSFEPTEKTSEGFDKMYYDYFPDAAKMIPKCSTQLKAVTAHFQTHTTPILLSNNFIEPL
                     EITKEIYDLNNPEKEPKKFQTAYAKKTGDQKGYREALCKWIDFTRDFLSKYTKTTSIDL
                     SSLRPSSQYKDLGEYYAELNPLLYHISFQRIAEKEIMDAVETGKLYLFQIYNKDFAKGH
                     HGKPNLHTLYWTGLFSPENLAKTSIKLNGQAELFYRPKSRMKRMAHRLGEKMLNKKLKD
                     QKTPIPDTLYQELYDYVNHRLSHDLSDEARALLPNVITKEVSHEIIKDRRFTSDKFFFH
                     VPITLNYQAANSPSKFNQRVNAYLKEHPETPIIGIDRGERNLIYITVIDSTGKILEQRS
                     LNTIQQFDYQKKLDNREKERVAARQAWSVVGTIKDLKQGYLSQVIHEIVDLMIHYQAVV
                     VLENLNFGFKSKRTGIAEKAVYQQFEKMLIDKLNCLVLKDYPAEKVGGVLNPYQLTDQF
                     TSFAKMGTQSGFLFYVPAPYTSKIDPLTGFVDPFVWKTIKNHESRKHFLEGFDFLHYDV
                     KTGDFILHFKMNRNLSFQRGLPGFMPAWDIVFEKNETQFDAKGTPFIAGKRIVPVIENH
                     RFTGRYRDLYPANELIALLEEKGIVFRDGSNILPKLLENDDSHAIDTMVALIRSVLQMR
                     NSNAATGEDYINSPVRDLNGVCFDSRFQNPEWPMDADANGAYHIALKGQLLLNHLKESK
                     DLKLQNGISNQDWLAYIQELRN"
     regulatory      9631..9640
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             9912..9959
                     /codon_start=1
                     /product="bipartite nuclear localization signal from 
                     nucleoplasmin"
                     /label=nucleoplasmin NLS
                     /translation="KRPAATKKAGQAKKKK"
     CDS             9975..10673
                     /codon_start=1
                     /product="monomeric blue fluorescent protein"
                     /label=TagBFP
                     /note="mammalian codon-optimized"
                     /translation="SELIKENMHMKLYMEGTVDNHHFKCTSEGEGKPYEGTQTMRIKVV
                     EGGPLPFAFDILATSFLYGSKTFINHTQGIPDFFKQSFPEGFTWERVTTYEDGGVLTAT
                     QDTSLQDGCLIYNVKIRGVNFTSNGPVMQKKTLGWEAFTETLYPADGGLEGRNDMALKL
                     VGGSHLIANIKTTYRSKKPAKNLKMPGVYYVDYRLERIKEANNETYVEQHEVAVARYCD
                     LPSKLGHKLN"
     polyA_signal    10725..10846
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(10736..10755)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     10790..10809
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     terminator      10961..11007
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     terminator      11037..11173
                     /label=rrnG terminator
                     /note="transcription terminator from the E. coli ribosomal 
                     RNA rrnG operon (Albrechtsen et al., 1991)"
     misc_feature    11218..11289
                     /label=3' beta-globin insulator
                     /note="insulator downstream of the human beta-globin locus 
                     (Farrell et al., 2002)"
     rep_origin      complement(11515..12103)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(11595..11614)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 gaagatcctt tgatcttttc tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa
       61 gggattttgg tcatgagatt atcaaaaagg atcttcacct agatcctttt aaattaaaaa
      121 tgaagtttta aatcaatcta aagtatatat gagtaaactt ggtctgacag ttaccaatgc
      181 ttaatcagtg aggcacctat ctcagcgatc tgtctatttc gttcatccat agttgcattt
      241 aaatttccga actctccaag gccctcgtcg gaaaatcttc aaacctttcg tccgatccat
      301 cttgcaggct acctctcgaa cgaactatcg caagtctctt ggccggcctt gcgccttggc
      361 tattgcttgg cagcgcctat cgccaggtat tactccaatc ccgaatatcc gagatcggga
      421 tcacccgaga gaagttcaac ctacatcctc aatcccgatc tatccgagat ccgaggaata
      481 tcgaaatcgg ggcgcgcctg gcctccgcgc cgggttttgg cgcctcccgc gggcgccccc
      541 ctcgtcacgg cgagcgctgc cacgtcagac gaagggcgca ggagcgtcct gatccttccg
      601 cccggacgct caggacagcg gcccgctgct cataagactc ggccttagaa ccccagtatc
      661 agcagaagga cattttagga cgggacttgg gtgactctag ggcactggtt ttctttccag
      721 agagcggaac aggcgaggaa aagtagtccc ttctcggcga ttctgcggag ggatctccgt
      781 ggggcggtga acgccgatga ttatataagg acgcgccggg tgtggcacag ctagttccgt
      841 cgcagccggg atttgggtcg cggttcttgt ttgtggatcg ctgtgatcgt cacttggtga
      901 gtagcgggct gctgggctgg ccggggcttt cgtggccgcc gggccgctcg gtgggacgga
      961 agcgtgtgga gagaccgcca agggctgtag tctgggtccg cgagcaaggt tgccctgaac
     1021 tgggggttgg ggggagcgca gcaaaatggc ggctgttccc gagtcttgaa tggaagacgc
     1081 ttgtgaggcg ggctgtgagg tcgttgaaac aaggtggggg gcatggtggg cggcaagaac
     1141 ccaaggtctt gagcccttcg ctaatgcggg aaagctctta ttcgggtgag atgggctggg
     1201 caccatctgg ggaccctgac gtgaagtttg tcactgactg gagaactcgg tttgtcgtct
     1261 gttgcggggg cggcagttat ggcggtgccg ttgggcagtg cacccgtacc tttgggagcg
     1321 cgcgccctcg tcgtgtcgtg acgtcacccg ttctgttggc ttataatgca gggtggggcc
     1381 acctgccggt aggtgtgcgg taggcttttc tccgtcgcag gacgcagggt tcgggcctag
     1441 ggtaggctct cctgaatcga caggcgccgg acctctggtg aggggaggga taagtgaggc
     1501 gtcagtttct ttggtcggtt ttatgtacct atcttcttaa gtagctgaag ctccggtttt
     1561 gaactatgcg ctcggggttg gcgagtgtgt tttgtgaagt tttttaggca ccttttgaaa
     1621 tgtaatcatt tgggtcaata tgtaattttc agtgttagac ttgtaaattg tccgctaaat
     1681 tctggccgtt tttggctttt ttgttagaca acatggccaa gcctttgtct caagaagaat
     1741 ccaccctcat tgaaagagca acggctacaa tcaacagcat ccccatctct gaagactaca
     1801 gcgtcgccag cgcagctctc tctagcgacg gccgcatctt cactggtgtc aatgtatatc
     1861 attttactgg gggaccttgt gcagaactcg tggtgctggg cactgctgct gctgcggcag
     1921 ctggcaacct gacttgtatc gtcgcgatcg gaaatgagaa caggggcatc ttgagcccct
     1981 gcggacggtg ccgacaggtg cttctcgatc tgcatcctgg gatcaaagcc atagtgaagg
     2041 acagtgatgg acagccgacg gcagttggga ttcgtgaatt gctgccctct ggttatgtgt
     2101 gggagggcta agctagtatg taagcctagt cttagataat aaaatcgcta tccatcgaag
     2161 atggatgtgt gttggttttt tgtgtgtgta acgctaggcg cgcctggtgt accgagaacg
     2221 atcctctcag tgcgagtctc gacgatccat atcgttgctt ggcagtcagc cagtcggaat
     2281 ccagcttggg acccaggaag tccaatcgtc agatattgta ctcaagcctg gtcacggcag
     2341 cgtaccgatc tgtttaaacc tagatattga tagtctgatc ggtcaacgta taatcgagtc
     2401 ctagcttttg caaacatcta tcaagagaca ggatcagcag gaggctttcg catgagtatt
     2461 caacatttcc gtgtcgccct tattcccttt tttgcggcat tttgccttcc tgtttttgct
     2521 cacccagaaa cgctggtgaa agtaaaagat gctgaagatc agttgggtgc gcgagtgggt
     2581 tacatcgaac tggatctcaa cagcggtaag atccttgaga gttttcgccc cgaagaacgc
     2641 tttccaatga tgagcacttt taaagttctg ctatgtggcg cggtattatc ccgtattgac
     2701 gccgggcaag agcaactcgg tcgccgcata cactattctc agaatgactt ggttgagtat
     2761 tcaccagtca cagaaaagca tcttacggat ggcatgacag taagagaatt atgcagtgct
     2821 gccataacca tgagtgataa cactgcggcc aacttacttc tgacaacgat tggaggaccg
     2881 aaggagctaa ccgctttttt gcacaacatg ggggatcatg taactcgcct tgatcgttgg
     2941 gaaccggagc tgaatgaagc cataccaaac gacgagcgtg acaccacgat gcctgtagca
     3001 atggcaacaa ccttgcgtaa actattaact ggcgaactac ttactctagc ttcccggcaa
     3061 cagttgatag actggatgga ggcggataaa gttgcaggac cacttctgcg ctcggccctt
     3121 ccggctggct ggtttattgc tgataaatct ggagccggtg agcgtgggtc tcgcggtatc
     3181 attgcagcac tggggccaga tggtaagccc tcccgtatcg tagttatcta cacgacgggg
     3241 agtcaggcaa ctatggatga acgaaataga cagatcgctg agataggtgc ctcactgatt
     3301 aagcattggt aaccgattct aggtgcattg gcgcagaaaa aaatgcctga tgcgacgctg
     3361 cgcgtcttat actcccacat atgccagatt cagcaacgga tacggcttcc ccaacttgcc
     3421 cacttccata cgtgtcctcc ttaccagaaa tttatcctta agatcccgaa tcgtttaaac
     3481 tcgactctgg ctctatcgaa tctccgtcgt ttcgagctta cgcgaacagc cgtggcgctc
     3541 atttgctcgt cgggcatcga atctcgtcag ctatcgtcag cttacctttt tggcagcgat
     3601 cgcggctccc gacatcttgg accattagct ccacaggtat cttcttccct ctagtggtca
     3661 taacagcagc ttcagctacc tctcaattca aaaaacccct caagacccgt ttagaggccc
     3721 caaggggtta tgctatcaat cgttgcgtta cacacacaaa aaaccaacac acatccatct
     3781 tcgatggata gcgattttat tatctaactg ctgatcgagt gtagccctta accgcttttg
     3841 aatgtccgtc ttagttaatc cctaattgaa cgctccaaaa ggtgacttcc aatagggatt
     3901 tattcctttt aaaattaacg gcataatcgt agatcgcatg ccgggaggtg gtccctgcag
     3961 ttacgccaat gataaccccc gccagaaaaa tcttagtagc cttccctttt tgttttccgt
     4021 gccccaactc ggcggattga ctcggcccct tccggaaaca cccgaatcaa cttctagtca
     4081 aattattgtt cacgccgcaa tgacccaccc ctggcccgcg tctgtggaac tgacccctgg
     4141 tgtacaggag agttcgctgc tgaaagtggt cccaaagggg tactagtttt taagctccca
     4201 actccccctc ccccagcgtc tggaggattc cacaccctcg caccgcaggg gcgaggaagt
     4261 gggcggagtc cggttttggc gccagccgct gaggctgcca agcagaaaag ccaccgctga
     4321 ggagactccg gtcactgtcc tcgccccgcc tcccccttcc ctccccttgg ggaccaccgg
     4381 gcgccacgcc gcgaacggta agtgccgcgg tcgtcggcgc ctccgccctc cccctagggc
     4441 cccaattccc agcgggcgcg gcgcgcggcc cctccccccg ccgggcgcgc gcccgctgcc
     4501 ccgcccttcg tggccgcccg gcgtgggcgg tgccacccct ccccccggcg gccccgcgcg
     4561 cagctcccgg ctccctcccc cttcggatgt ggcttgagct gtaggcgcgg aggggctagc
     4621 tcgtgaggct ccggtgcccg tcagtgggca gagcgcacat cgcccacagt ccccgagaag
     4681 ttggggggag gggtcggcaa ttgaaccggt gcctagagaa ggtggcgcgg ggtaaactgg
     4741 gaaagtgatg tcgtgtactg gctccgcctt tttcccgagg gtgggggaga accgtatata
     4801 agtgcactag tcgccgtgaa cgttcttttt cgcaacgggt ttgccgccag aacacaggta
     4861 agtgccgtgt gtggttcccg cgggcctggc ctctttacgg gttatggccc ttgcgtgcct
     4921 tgaattactt ccacctggct gcagtacgtg attcttgatc ccgagcttcg ggttggaagt
     4981 gggtgggaga gttcgtggcc ttgcgcttaa ggagcccctt cgcctcgtgc ttgagttgtg
     5041 gcctggcctg ggcgctgggg ccgccgcgtg cgaatctggt ggcaccttcg cgcctgtctc
     5101 gctgctttcg ataagtctct agccatttaa aatttttgat gacctgctgc gacgcttttt
     5161 ttctggcaag atagtcttgt aaatgcgggc caagatcagc acactggtat ttcggttttt
     5221 ggggccgcgg gcggcgacgg ggcccgtgcg tcccagcgca catgttcggc gaggcggggc
     5281 ctgcgagcgc ggccaccgag aatcggacgg gggtagtctc aagctgcccg gcctgctctg
     5341 gtgcctggcc tcgcgccgcc gtgtatcgcc ccgccctggg cggcaaggct ggcccggtcg
     5401 gcaccagttg cgtgagcgga aagatggccg cttcccggcc ctgctgcagg gagcacaaaa
     5461 tggaggacgc ggcgctcggg agagcgggcg ggtgagtcac ccacacaaag gaaaagggcc
     5521 tttccgtcct cagccgtcgc ttcatgtgac tccacggagt accgggcgcc gtccaggcac
     5581 ctcgattagt tctccagctt ttggagtacg tcgtctttag gttgggggga ggggttttat
     5641 gcgatggagt ttccccacac tgagtgggtg gagactgaag ttaggccagc ttggcacttg
     5701 atgtaattct ccttggaatt tgcccttttt gagtttggat cttggttcat tctcaagcct
     5761 cagacagtgg ttcaaagttt ttttcttcca tttcaggtgt cgtgaaaact acccctaaaa
     5821 gccaaaagat ctttgtcgat cctaccatcc actcgacaca cccgccagcg gccgctgcca
     5881 agcttgctag gaattccgcg tctgcaggat atcaagcttg cggtaccgcg ggcccggcca
     5941 ccatggcccc aaagaagaag cggaaggtcg gtatccacgg agtcccagca gccacacagt
     6001 tcgagggctt taccaacctg tatcaggtga gcaagacact gcggtttgag ctgatcccac
     6061 agggcaagac cctgaagcac atccaggagc agggcttcat cgaggaggac aaggcccgca
     6121 atgatcacta caaggagctg aagcccatca tcgatcggat ctacaagacc tatgccgacc
     6181 agtgcctgca gctggtgcag ctggattggg agaacctgag cgccgccatc gactcctata
     6241 gaaaggagaa aaccgaggag acaaggaacg ccctgatcga ggagcaggcc acatatcgca
     6301 atgccatcca cgactacttc atcggccgga cagacaacct gaccgatgcc atcaataaga
     6361 gacacgccga gatctacaag ggcctgttca aggccgagct gtttaatggc aaggtgctga
     6421 agcagctggg caccgtgacc acaaccgagc acgagaacgc cctgctgcgg agcttcgaca
     6481 agtttacaac ctacttctcc ggcttttatc ggaacaggaa gaacgtgttc agcgccgagg
     6541 atatcagcac agccatccca caccgcatcg tgcaggacaa cttccccaag tttaaggaga
     6601 attgtcacat cttcacacgc ctgatcaccg ccgtgcccag cctgcgggag cactttgaga
     6661 acgtgaagaa ggccatcggc atcttcgtga gcacctccat cgaggaggtg ttttccttcc
     6721 ctttttataa ccagctgctg acacagaccc agatcgacct gtataaccag ctgctgggag
     6781 gaatctctcg ggaggcaggc accgagaaga tcaagggcct gaacgaggtg ctgaatctgg
     6841 ccatccagaa gaatgatgag acagcccaca tcatcgcctc cctgccacac agattcatcc
     6901 ccctgtttaa gcagatcctg tccgatagga acaccctgtc tttcatcctg gaggagttta
     6961 agagcgacga ggaagtgatc cagtccttct gcaagtacaa gacactgctg agaaacgaga
     7021 acgtgctgga gacagccgag gccctgttta acgagctgaa cagcatcgac ctgacacaca
     7081 tcttcatcag ccacaagaag ctggagacaa tcagcagcgc cctgtgcgac cactgggata
     7141 cactgaggaa tgccctgtat gagcggagaa tctccgagct gacaggcaag atcaccaagt
     7201 ctgccaagga gaaggtgcag cgcagcctga agcacgagga tatcaacctg caggagatca
     7261 tctctgccgc aggcaaggag ctgagcgagg ccttcaagca gaaaaccagc gagatcctgt
     7321 cccacgcaca cgccgccctg gatcagccac tgcctacaac cctgaagaag caggaggaga
     7381 aggagatcct gaagtctcag ctggacagcc tgctgggcct gtaccacctg ctggactggt
     7441 ttgccgtgga tgagtccaac gaggtggacc ccgagttctc tgcccggctg accggcatca
     7501 agctggagat ggagccttct ctgagcttct acaacaaggc cagaaattat gccaccaaga
     7561 agccctactc cgtggagaag ttcaagctga actttcagat gcctacactg gcccgcggct
     7621 gggacgtgaa tcgggagaag aacaatggcg ccatcctgtt tgtgaagaac ggcctgtact
     7681 atctgggcat catgccaaag cagaagggca ggtataaggc cctgagcttc gagcccacag
     7741 agaaaaccag cgagggcttt gataagatgt actatgacta cttccctgat gccgccaaga
     7801 tgatcccaaa gtgcagcacc cagctgaagg ccgtgacagc ccactttcag acccacacaa
     7861 cccccatcct gctgtccaac aatttcatcg agcctctgga gatcacaaag gagatctacg
     7921 acctgaacaa tcctgagaag gagccaaaga agtttcagac agcctacgcc aagaaaaccg
     7981 gcgaccagaa gggctacaga gaggccctgt gcaagtggat cgacttcaca agggattttc
     8041 tgtccaagta taccaagaca acctctatcg atctgtctag cctgcggcca tcctctcagt
     8101 ataaggacct gggcgagtac tatgccgagc tgaatcccct gctgtaccac atcagcttcc
     8161 agagaatcgc cgagaaggag atcatggatg ccgtggagac aggcaagctg tacctgttcc
     8221 agatctataa caaggacttt gccaagggcc accacggcaa gcctaatctg cacacactgt
     8281 attggaccgg cctgttttct ccagagaacc tggccaagac aagcatcaag ctgaatggcc
     8341 aggccgagct gttctaccgc cctaagtcca ggatgaagag gatggcacac cggctgggag
     8401 agaagatgct gaacaagaag ctgaaggatc agaaaacccc aatccccgac accctgtacc
     8461 aggagctgta cgactatgtg aatcacagac tgtcccacga cctgtctgat gaggccaggg
     8521 ccctgctgcc caacgtgatc accaaggagg tgtctcacga gatcatcaag gataggcgct
     8581 ttaccagcga caagttcttt ttccacgtgc ctatcacact gaactatcag gccgccaatt
     8641 ccccatctaa gttcaaccag agggtgaatg cctacctgaa ggagcacccc gagacaccta
     8701 tcatcggcat cgatcggggc gagagaaacc tgatctatat cacagtgatc gactccaccg
     8761 gcaagatcct ggagcagcgg agcctgaaca ccatccagca gtttgattac cagaagaagc
     8821 tggacaacag ggagaaggag agggtggcag caaggcaggc ctggtctgtg gtgggcacaa
     8881 tcaaggatct gaagcagggc tatctgagcc aggtcatcca cgagatcgtg gacctgatga
     8941 tccactacca ggccgtggtg gtgctggaga acctgaattt cggctttaag agcaagagga
     9001 ccggcatcgc cgagaaggcc gtgtaccagc agttcgagaa gatgctgatc gataagctga
     9061 attgcctggt gctgaaggac tatccagcag agaaagtggg aggcgtgctg aacccatacc
     9121 agctgacaga ccagttcacc tcctttgcca agatgggcac ccagtctggc ttcctgtttt
     9181 acgtgcctgc cccatataca tctaagatcg atcccctgac cggcttcgtg gaccccttcg
     9241 tgtggaaaac catcaagaat cacgagagcc gcaagcactt cctggagggc ttcgactttc
     9301 tgcactacga cgtgaaaacc ggcgacttca tcctgcactt taagatgaac agaaatctgt
     9361 ccttccagag gggcctgccc ggctttatgc ctgcatggga tatcgtgttc gagaagaacg
     9421 agacacagtt tgacgccaag ggcacccctt tcatcgccgg caagagaatc gtgccagtga
     9481 tcgagaatca cagattcacc ggcagatacc gggacctgta tcctgccaac gagctgatcg
     9541 ccctgctgga ggagaagggc atcgtgttca gggatggctc caacatcctg ccaaagctgc
     9601 tggagaatga cgattctcac gccatcgaca ccatggtggc cctgatccgc agcgtgctgc
     9661 agatgcggaa ctccaatgcc gccacaggcg aggactatat caacagcccc gtgcgcgatc
     9721 tgaatggcgt gtgcttcgac tcccggtttc agaacccaga gtggcccatg gacgccgatg
     9781 ccaatggcgc ctaccacatc gccctgaagg gccagctgct gctgaatcac ctgaaggaga
     9841 gcaaggatct gaagctgcag aacggcatct ccaatcagga ctggctggcc tacatccagg
     9901 agctgcgcaa caaaaggccg gcggccacga aaaaggccgg ccaggcaaaa aagaaaaagg
     9961 gcagcagcgg atccagcgag ctgattaagg agaacatgca catgaagctg tacatggagg
    10021 gcaccgtgga caaccatcac ttcaagtgca catccgaggg cgaaggcaag ccctacgagg
    10081 gcacccagac catgagaatc aaggtggtcg agggcggccc tctccccttc gccttcgaca
    10141 tcctggctac tagcttcctc tacggcagca agaccttcat caaccacacc cagggcatcc
    10201 ccgacttctt caagcagtcc ttccctgagg gcttcacatg ggagagagtc accacatacg
    10261 aagacggggg cgtgctgacc gctacccagg acaccagcct ccaggacggc tgcctcatct
    10321 acaacgtcaa gatcagaggg gtgaacttca catccaacgg ccctgtgatg cagaagaaaa
    10381 cactcggctg ggaggccttc accgagacgc tgtaccccgc tgacggcggc ctggaaggca
    10441 gaaacgacat ggccctgaag ctcgtgggcg ggagccatct gatcgcaaac atcaagacca
    10501 catatagatc caagaaaccc gctaagaacc tcaagatgcc tggcgtctac tatgtggact
    10561 acagactgga aagaatcaag gaggccaaca acgagaccta cgtcgagcag cacgaggtgg
    10621 cagtggccag atactgcgac ctccctagca aactggggca caagcttaat tagtaactag
    10681 cttgactgac tgagatacag cgtaccttca gctcacagac atgataagat acattgatga
    10741 gtttggacaa accacaacta gaatgcagtg aaaaaaatgc tttatttgtg aaatttgtga
    10801 tgctattgct ttatttgtaa ccattataag ctgcaataaa caagttaaca acaacaattg
    10861 cattcatttt atgtttcagg ttcaggggga ggtgtgggag gttttttaaa gcaagtaaaa
    10921 cctctacaaa tgtggtattg gcccatctct atcggtatcg tagcataacc ccttggggcc
    10981 tctaaacggg tcttgagggg ttttttgtgc ccctcgggcc ggattgctat ctaccggcat
    11041 tggcgcagaa aaaaatgcct gatgcgacgc tgcgcgtctt atactcccac atatgccaga
    11101 ttcagcaacg gatacggctt ccccaacttg cccacttcca tacgtgtcct ccttaccaga
    11161 aatttatcct taaggtcgtc agctatcctg caggcgatct ctcgatttcg atcaagacat
    11221 tcctttaatg gtcttttctg gacaccacta ggggtcagaa gtagttcatc aaactttctt
    11281 ccctccctaa tctcattggt taccttgggc tatcgaaact taattaacca gtcaagtcag
    11341 ctacttggcg agatcgactt gtctgggttt cgactacgct cagaattgcg tcagtcaagt
    11401 tcgatctggt ccttgctatt gcacccgttc tccgattacg agtttcattt aaatcatgtg
    11461 agcaaaaggc cagcaaaagg ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca
    11521 taggctccgc ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa
    11581 cccgacagga ctataaagat accaggcgtt tccccctgga agctccctcg tgcgctctcc
    11641 tgttccgacc ctgccgctta ccggatacct gtccgccttt ctcccttcgg gaagcgtggc
    11701 gctttctcat agctcacgct gtaggtatct cagttcggtg taggtcgttc gctccaagct
    11761 gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc gccttatccg gtaactatcg
    11821 tcttgagtcc aacccggtaa gacacgactt atcgccactg gcagcagcca ctggtaacag
    11881 gattagcaga gcgaggtatg taggcggtgc tacagagttc ttgaagtggt ggcctaacta
    11941 cggctacact agaagaacag tatttggtat ctgcgctctg ctgaagccag ttaccttcgg
    12001 aaaaagagtt ggtagctctt gatccggcaa acaaaccacc gctggtagcg gtggtttttt
    12061 tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct caa
//
