LOCUS       sequence-496441-       11418 bp    DNA     circular SYN 07-JAN-2026
DEFINITION  Overexpression of EnAsCas12a-BFP with blasticidin resistance gene. 
            Contains Cp36 recombination site..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11418)
  AUTHORS   Arana S, Du PP, Vaughan-Jackson A, Enright N, Spees K, Valbuena R, 
            Garcia CA, Nguyen T, Venida A, Seczynska M, Bintu L, Lehner PJ, 
            Prolo LM, Bassik MC
  TITLE     Reduced Cas9 transgene silencing by incorporation of intron 
            sequences.
  JOURNAL   Nat Commun. 2025 Nov 27;16(1):10656. doi: 
            10.1038/s41467-025-65669-0.
  PUBMED    41309578
REFERENCE   2  (bases 1 to 11418)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Jan 7, 2026 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2025-11-27"; volume: "16(1):10656. doi"; pages: " 
            10.1038/s41467-025-65669-0"
COMMENT     Sequence Label: pPD684 EnAsCas12a-BFP
FEATURES             Location/Qualifiers
     source          1..11418
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        500..1709
                     /label=UbC promoter
                     /note="human ubiquitin C promoter"
     primer_bind     1546..1565
                     /label=hUBCpro-F
                     /note="Human Ubiquitin C (UbC) promoter, forward primer"
     CDS             1713..2111
                     /codon_start=1
                     /gene="Aspergillus terreus BSD"
                     /product="blasticidin S deaminase"
                     /label=BSD
                     /note="confers resistance to blasticidin"
                     /translation="MAKPLSQEESTLIERATATINSIPISEDYSVASAALSSDGRIFTG
                     VNVYHFTGGPCAELVVLGTAAAAAAGNLTCIVAIGNENRGILSPCGRCRQVLLDLHPGI
                     KAIVKDSDGQPTAVGIRELLPSGYVWEG"
     polyA_signal    2138..2186
                     /note="synthetic polyadenylation signal"
     CDS             join(2452..2520,2521..3312)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(2670..2689)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     terminator      3325..3461
                     /label=rrnG terminator
                     /note="transcription terminator from the E. coli ribosomal 
                     RNA rrnG operon (Albrechtsen et al., 1991)"
     misc_feature    3613..3684
                     /label=5' beta-globin insulator
                     /note="insulator upstream of the human beta-globin locus 
                     (Farrell et al., 2002)"
     terminator      complement(3689..3735)
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     polyA_signal    3753..3801
                     /note="synthetic polyadenylation signal"
     promoter        3942..5120
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          4173..5111
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     5068..5088
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     regulatory      5252..5261
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             5264..5284
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             5309..9226
                     /codon_start=1
                     /product="CRISPR-associated protein from Acidaminococcus 
                     sp. BV3L6 (Zetsche et al., 2015)"
                     /label=AsCpf1
                     /translation="TQFEGFTNLYQVSKTLRFELIPQGKTLKHIQEQGFIEEDKARNDH
                     YKELKPIIDRIYKTYADQCLQLVQLDWENLSAAIDSYRKEKTEETRNALIEEQATYRNA
                     IHDYFIGRTDNLTDAINKRHAEIYKGLFKAELFNGKVLKQLGTVTTTEHENALLRSFDK
                     FTTYFSGFYRNRKNVFSAEDISTAIPHRIVQDNFPKFKENCHIFTRLITAVPSLREHFE
                     NVKKAIGIFVSTSIEEVFSFPFYNQLLTQTQIDLYNQLLGGISREAGTEKIKGLNEVLN
                     LAIQKNDETAHIIASLPHRFIPLFKQILSDRNTLSFILEEFKSDEEVIQSFCKYKTLLR
                     NENVLETAEALFNELNSIDLTHIFISHKKLETISSALCDHWDTLRNALYERRISELTGK
                     ITKSAKEKVQRSLKHEDINLQEIISAAGKELSEAFKQKTSEILSHAHAALDQPLPTTLK
                     KQEEKEILKSQLDSLLGLYHLLDWFAVDESNEVDPEFSARLTGIKLEMEPSLSFYNKAR
                     NYATKKPYSVEKFKLNFQMPTLARGWDVNREKNNGAILFVKNGLYYLGIMPKQKGRYKA
                     LSFEPTEKTSEGFDKMYYDYFPDAAKMIPKCSTQLKAVTAHFQTHTTPILLSNNFIEPL
                     EITKEIYDLNNPEKEPKKFQTAYAKKTGDQKGYREALCKWIDFTRDFLSKYTKTTSIDL
                     SSLRPSSQYKDLGEYYAELNPLLYHISFQRIAEKEIMDAVETGKLYLFQIYNKDFAKGH
                     HGKPNLHTLYWTGLFSPENLAKTSIKLNGQAELFYRPKSRMKRMAHRLGEKMLNKKLKD
                     QKTPIPDTLYQELYDYVNHRLSHDLSDEARALLPNVITKEVSHEIIKDRRFTSDKFFFH
                     VPITLNYQAANSPSKFNQRVNAYLKEHPETPIIGIDRGERNLIYITVIDSTGKILEQRS
                     LNTIQQFDYQKKLDNREKERVAARQAWSVVGTIKDLKQGYLSQVIHEIVDLMIHYQAVV
                     VLENLNFGFKSKRTGIAEKAVYQQFEKMLIDKLNCLVLKDYPAEKVGGVLNPYQLTDQF
                     TSFAKMGTQSGFLFYVPAPYTSKIDPLTGFVDPFVWKTIKNHESRKHFLEGFDFLHYDV
                     KTGDFILHFKMNRNLSFQRGLPGFMPAWDIVFEKNETQFDAKGTPFIAGKRIVPVIENH
                     RFTGRYRDLYPANELIALLEEKGIVFRDGSNILPKLLENDDSHAIDTMVALIRSVLQMR
                     NSNAATGEDYINSPVRDLNGVCFDSRFQNPEWPMDADANGAYHIALKGQLLLNHLKESK
                     DLKLQNGISNQDWLAYIQELRN"
     regulatory      8946..8955
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             9227..9274
                     /codon_start=1
                     /product="bipartite nuclear localization signal from 
                     nucleoplasmin"
                     /label=nucleoplasmin NLS
                     /translation="KRPAATKKAGQAKKKK"
     CDS             9290..9988
                     /codon_start=1
                     /product="monomeric blue fluorescent protein"
                     /label=TagBFP
                     /note="mammalian codon-optimized"
                     /translation="SELIKENMHMKLYMEGTVDNHHFKCTSEGEGKPYEGTQTMRIKVV
                     EGGPLPFAFDILATSFLYGSKTFINHTQGIPDFFKQSFPEGFTWERVTTYEDGGVLTAT
                     QDTSLQDGCLIYNVKIRGVNFTSNGPVMQKKTLGWEAFTETLYPADGGLEGRNDMALKL
                     VGGSHLIANIKTTYRSKKPAKNLKMPGVYYVDYRLERIKEANNETYVEQHEVAVARYCD
                     LPSKLGHKLN"
     polyA_signal    10040..10161
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(10051..10070)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     10105..10124
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     terminator      10276..10322
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     terminator      10352..10488
                     /label=rrnG terminator
                     /note="transcription terminator from the E. coli ribosomal 
                     RNA rrnG operon (Albrechtsen et al., 1991)"
     misc_feature    10533..10604
                     /label=3' beta-globin insulator
                     /note="insulator downstream of the human beta-globin locus 
                     (Farrell et al., 2002)"
     rep_origin      complement(10830..11418)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(10910..10929)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 gaagatcctt tgatcttttc tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa
       61 gggattttgg tcatgagatt atcaaaaagg atcttcacct agatcctttt aaattaaaaa
      121 tgaagtttta aatcaatcta aagtatatat gagtaaactt ggtctgacag ttaccaatgc
      181 ttaatcagtg aggcacctat ctcagcgatc tgtctatttc gttcatccat agttgcattt
      241 aaatttccga actctccaag gccctcgtcg gaaaatcttc aaacctttcg tccgatccat
      301 cttgcaggct acctctcgaa cgaactatcg caagtctctt ggccggcctt gcgccttggc
      361 tattgcttgg cagcgcctat cgccaggtat tactccaatc ccgaatatcc gagatcggga
      421 tcacccgaga gaagttcaac ctacatcctc aatcccgatc tatccgagat ccgaggaata
      481 tcgaaatcgg ggcgcgcctg gcctccgcgc cgggttttgg cgcctcccgc gggcgccccc
      541 ctcgtcacgg cgagcgctgc cacgtcagac gaagggcgca ggagcgtcct gatccttccg
      601 cccggacgct caggacagcg gcccgctgct cataagactc ggccttagaa ccccagtatc
      661 agcagaagga cattttagga cgggacttgg gtgactctag ggcactggtt ttctttccag
      721 agagcggaac aggcgaggaa aagtagtccc ttctcggcga ttctgcggag ggatctccgt
      781 ggggcggtga acgccgatga ttatataagg acgcgccggg tgtggcacag ctagttccgt
      841 cgcagccggg atttgggtcg cggttcttgt ttgtggatcg ctgtgatcgt cacttggtga
      901 gtagcgggct gctgggctgg ccggggcttt cgtggccgcc gggccgctcg gtgggacgga
      961 agcgtgtgga gagaccgcca agggctgtag tctgggtccg cgagcaaggt tgccctgaac
     1021 tgggggttgg ggggagcgca gcaaaatggc ggctgttccc gagtcttgaa tggaagacgc
     1081 ttgtgaggcg ggctgtgagg tcgttgaaac aaggtggggg gcatggtggg cggcaagaac
     1141 ccaaggtctt gagcccttcg ctaatgcggg aaagctctta ttcgggtgag atgggctggg
     1201 caccatctgg ggaccctgac gtgaagtttg tcactgactg gagaactcgg tttgtcgtct
     1261 gttgcggggg cggcagttat ggcggtgccg ttgggcagtg cacccgtacc tttgggagcg
     1321 cgcgccctcg tcgtgtcgtg acgtcacccg ttctgttggc ttataatgca gggtggggcc
     1381 acctgccggt aggtgtgcgg taggcttttc tccgtcgcag gacgcagggt tcgggcctag
     1441 ggtaggctct cctgaatcga caggcgccgg acctctggtg aggggaggga taagtgaggc
     1501 gtcagtttct ttggtcggtt ttatgtacct atcttcttaa gtagctgaag ctccggtttt
     1561 gaactatgcg ctcggggttg gcgagtgtgt tttgtgaagt tttttaggca ccttttgaaa
     1621 tgtaatcatt tgggtcaata tgtaattttc agtgttagac ttgtaaattg tccgctaaat
     1681 tctggccgtt tttggctttt ttgttagaca acatggccaa gcctttgtct caagaagaat
     1741 ccaccctcat tgaaagagca acggctacaa tcaacagcat ccccatctct gaagactaca
     1801 gcgtcgccag cgcagctctc tctagcgacg gccgcatctt cactggtgtc aatgtatatc
     1861 attttactgg gggaccttgt gcagaactcg tggtgctggg cactgctgct gctgcggcag
     1921 ctggcaacct gacttgtatc gtcgcgatcg gaaatgagaa caggggcatc ttgagcccct
     1981 gcggacggtg ccgacaggtg cttctcgatc tgcatcctgg gatcaaagcc atagtgaagg
     2041 acagtgatgg acagccgacg gcagttggga ttcgtgaatt gctgccctct ggttatgtgt
     2101 gggagggcta agctagtatg taagcctagt cttagataat aaaatcgcta tccatcgaag
     2161 atggatgtgt gttggttttt tgtgtgtgta acgctaggcg cgcctggtgt accgagaacg
     2221 atcctctcag tgcgagtctc gacgatccat atcgttgctt ggcagtcagc cagtcggaat
     2281 ccagcttggg acccaggaag tccaatcgtc agatattgta ctcaagcctg gtcacggcag
     2341 cgtaccgatc tgtttaaacc tagatattga tagtctgatc ggtcaacgta taatcgagtc
     2401 ctagcttttg caaacatcta tcaagagaca ggatcagcag gaggctttcg catgagtatt
     2461 caacatttcc gtgtcgccct tattcccttt tttgcggcat tttgccttcc tgtttttgct
     2521 cacccagaaa cgctggtgaa agtaaaagat gctgaagatc agttgggtgc gcgagtgggt
     2581 tacatcgaac tggatctcaa cagcggtaag atccttgaga gttttcgccc cgaagaacgc
     2641 tttccaatga tgagcacttt taaagttctg ctatgtggcg cggtattatc ccgtattgac
     2701 gccgggcaag agcaactcgg tcgccgcata cactattctc agaatgactt ggttgagtat
     2761 tcaccagtca cagaaaagca tcttacggat ggcatgacag taagagaatt atgcagtgct
     2821 gccataacca tgagtgataa cactgcggcc aacttacttc tgacaacgat tggaggaccg
     2881 aaggagctaa ccgctttttt gcacaacatg ggggatcatg taactcgcct tgatcgttgg
     2941 gaaccggagc tgaatgaagc cataccaaac gacgagcgtg acaccacgat gcctgtagca
     3001 atggcaacaa ccttgcgtaa actattaact ggcgaactac ttactctagc ttcccggcaa
     3061 cagttgatag actggatgga ggcggataaa gttgcaggac cacttctgcg ctcggccctt
     3121 ccggctggct ggtttattgc tgataaatct ggagccggtg agcgtgggtc tcgcggtatc
     3181 attgcagcac tggggccaga tggtaagccc tcccgtatcg tagttatcta cacgacgggg
     3241 agtcaggcaa ctatggatga acgaaataga cagatcgctg agataggtgc ctcactgatt
     3301 aagcattggt aaccgattct aggtgcattg gcgcagaaaa aaatgcctga tgcgacgctg
     3361 cgcgtcttat actcccacat atgccagatt cagcaacgga tacggcttcc ccaacttgcc
     3421 cacttccata cgtgtcctcc ttaccagaaa tttatcctta agatcccgaa tcgtttaaac
     3481 tcgactctgg ctctatcgaa tctccgtcgt ttcgagctta cgcgaacagc cgtggcgctc
     3541 atttgctcgt cgggcatcga atctcgtcag ctatcgtcag cttacctttt tggcagcgat
     3601 cgcggctccc gacatcttgg accattagct ccacaggtat cttcttccct ctagtggtca
     3661 taacagcagc ttcagctacc tctcaattca aaaaacccct caagacccgt ttagaggccc
     3721 caaggggtta tgctatcaat cgttgcgtta cacacacaaa aaaccaacac acatccatct
     3781 tcgatggata gcgattttat tatctaactg ctgatcgagt gtagccctta accgcttttg
     3841 aatgtccgtc ttagttaatc cctaattgaa cgctccaaaa ggtgacttcc aatagggatt
     3901 tattcctttt aaaattaacg gcataatcgt agatctcgtg aggctccggt gcccgtcagt
     3961 gggcagagcg cacatcgccc acagtccccg agaagttggg gggaggggtc ggcaattgaa
     4021 ccggtgccta gagaaggtgg cgcggggtaa actgggaaag tgatgtcgtg tactggctcc
     4081 gcctttttcc cgagggtggg ggagaaccgt atataagtgc actagtcgcc gtgaacgttc
     4141 tttttcgcaa cgggtttgcc gccagaacac aggtaagtgc cgtgtgtggt tcccgcgggc
     4201 ctggcctctt tacgggttat ggcccttgcg tgccttgaat tacttccacc tggctgcagt
     4261 acgtgattct tgatcccgag cttcgggttg gaagtgggtg ggagagttcg tggccttgcg
     4321 cttaaggagc cccttcgcct cgtgcttgag ttgtggcctg gcctgggcgc tggggccgcc
     4381 gcgtgcgaat ctggtggcac cttcgcgcct gtctcgctgc tttcgataag tctctagcca
     4441 tttaaaattt ttgatgacct gctgcgacgc tttttttctg gcaagatagt cttgtaaatg
     4501 cgggccaaga tcagcacact ggtatttcgg tttttggggc cgcgggcggc gacggggccc
     4561 gtgcgtccca gcgcacatgt tcggcgaggc ggggcctgcg agcgcggcca ccgagaatcg
     4621 gacgggggta gtctcaagct gcccggcctg ctctggtgcc tggcctcgcg ccgccgtgta
     4681 tcgccccgcc ctgggcggca aggctggccc ggtcggcacc agttgcgtga gcggaaagat
     4741 ggccgcttcc cggccctgct gcagggagca caaaatggag gacgcggcgc tcgggagagc
     4801 gggcgggtga gtcacccaca caaaggaaaa gggcctttcc gtcctcagcc gtcgcttcat
     4861 gtgactccac ggagtaccgg gcgccgtcca ggcacctcga ttagttctcc agcttttgga
     4921 gtacgtcgtc tttaggttgg ggggaggggt tttatgcgat ggagtttccc cacactgagt
     4981 gggtggagac tgaagttagg ccagcttggc acttgatgta attctccttg gaatttgccc
     5041 tttttgagtt tggatcttgg ttcattctca agcctcagac agtggttcaa agtttttttc
     5101 ttccatttca ggtgtcgtga aaactacccc taaaagccaa aagatctttg tcgatcctac
     5161 catccactcg acacacccgc cagcggccgc tgccaagctt gctaggaatt ccgcgtctgc
     5221 aggatatcaa gcttgcggta ccgcgggccc ggccaccatg gccccaaaga agaagcggaa
     5281 ggtcggtatc cacggagtcc cagcagccac acagttcgag ggctttacca acctgtatca
     5341 ggtgagcaag acactgcggt ttgagctgat cccacagggc aagaccctga agcacatcca
     5401 ggagcagggc ttcatcgagg aggacaaggc ccgcaatgat cactacaagg agctgaagcc
     5461 catcatcgat cggatctaca agacctatgc cgaccagtgc ctgcagctgg tgcagctgga
     5521 ttgggagaac ctgagcgccg ccatcgactc ctatagaaag gagaaaaccg aggagacaag
     5581 gaacgccctg atcgaggagc aggccacata tcgcaatgcc atccacgact acttcatcgg
     5641 ccggacagac aacctgaccg atgccatcaa taagagacac gccgagatct acaagggcct
     5701 gttcaaggcc gagctgttta atggcaaggt gctgaagcag ctgggcaccg tgaccacaac
     5761 cgagcacgag aacgccctgc tgcggagctt cgacaagttt acaacctact tctccggctt
     5821 ttatcggaac aggaagaacg tgttcagcgc cgaggatatc agcacagcca tcccacaccg
     5881 catcgtgcag gacaacttcc ccaagtttaa ggagaattgt cacatcttca cacgcctgat
     5941 caccgccgtg cccagcctgc gggagcactt tgagaacgtg aagaaggcca tcggcatctt
     6001 cgtgagcacc tccatcgagg aggtgttttc cttccctttt tataaccagc tgctgacaca
     6061 gacccagatc gacctgtata accagctgct gggaggaatc tctcgggagg caggcaccga
     6121 gaagatcaag ggcctgaacg aggtgctgaa tctggccatc cagaagaatg atgagacagc
     6181 ccacatcatc gcctccctgc cacacagatt catccccctg tttaagcaga tcctgtccga
     6241 taggaacacc ctgtctttca tcctggagga gtttaagagc gacgaggaag tgatccagtc
     6301 cttctgcaag tacaagacac tgctgagaaa cgagaacgtg ctggagacag ccgaggccct
     6361 gtttaacgag ctgaacagca tcgacctgac acacatcttc atcagccaca agaagctgga
     6421 gacaatcagc agcgccctgt gcgaccactg ggatacactg aggaatgccc tgtatgagcg
     6481 gagaatctcc gagctgacag gcaagatcac caagtctgcc aaggagaagg tgcagcgcag
     6541 cctgaagcac gaggatatca acctgcagga gatcatctct gccgcaggca aggagctgag
     6601 cgaggccttc aagcagaaaa ccagcgagat cctgtcccac gcacacgccg ccctggatca
     6661 gccactgcct acaaccctga agaagcagga ggagaaggag atcctgaagt ctcagctgga
     6721 cagcctgctg ggcctgtacc acctgctgga ctggtttgcc gtggatgagt ccaacgaggt
     6781 ggaccccgag ttctctgccc ggctgaccgg catcaagctg gagatggagc cttctctgag
     6841 cttctacaac aaggccagaa attatgccac caagaagccc tactccgtgg agaagttcaa
     6901 gctgaacttt cagatgccta cactggcccg cggctgggac gtgaatcggg agaagaacaa
     6961 tggcgccatc ctgtttgtga agaacggcct gtactatctg ggcatcatgc caaagcagaa
     7021 gggcaggtat aaggccctga gcttcgagcc cacagagaaa accagcgagg gctttgataa
     7081 gatgtactat gactacttcc ctgatgccgc caagatgatc ccaaagtgca gcacccagct
     7141 gaaggccgtg acagcccact ttcagaccca cacaaccccc atcctgctgt ccaacaattt
     7201 catcgagcct ctggagatca caaaggagat ctacgacctg aacaatcctg agaaggagcc
     7261 aaagaagttt cagacagcct acgccaagaa aaccggcgac cagaagggct acagagaggc
     7321 cctgtgcaag tggatcgact tcacaaggga ttttctgtcc aagtatacca agacaacctc
     7381 tatcgatctg tctagcctgc ggccatcctc tcagtataag gacctgggcg agtactatgc
     7441 cgagctgaat cccctgctgt accacatcag cttccagaga atcgccgaga aggagatcat
     7501 ggatgccgtg gagacaggca agctgtacct gttccagatc tataacaagg actttgccaa
     7561 gggccaccac ggcaagccta atctgcacac actgtattgg accggcctgt tttctccaga
     7621 gaacctggcc aagacaagca tcaagctgaa tggccaggcc gagctgttct accgccctaa
     7681 gtccaggatg aagaggatgg cacaccggct gggagagaag atgctgaaca agaagctgaa
     7741 ggatcagaaa accccaatcc ccgacaccct gtaccaggag ctgtacgact atgtgaatca
     7801 cagactgtcc cacgacctgt ctgatgaggc cagggccctg ctgcccaacg tgatcaccaa
     7861 ggaggtgtct cacgagatca tcaaggatag gcgctttacc agcgacaagt tctttttcca
     7921 cgtgcctatc acactgaact atcaggccgc caattcccca tctaagttca accagagggt
     7981 gaatgcctac ctgaaggagc accccgagac acctatcatc ggcatcgatc ggggcgagag
     8041 aaacctgatc tatatcacag tgatcgactc caccggcaag atcctggagc agcggagcct
     8101 gaacaccatc cagcagtttg attaccagaa gaagctggac aacagggaga aggagagggt
     8161 ggcagcaagg caggcctggt ctgtggtggg cacaatcaag gatctgaagc agggctatct
     8221 gagccaggtc atccacgaga tcgtggacct gatgatccac taccaggccg tggtggtgct
     8281 ggagaacctg aatttcggct ttaagagcaa gaggaccggc atcgccgaga aggccgtgta
     8341 ccagcagttc gagaagatgc tgatcgataa gctgaattgc ctggtgctga aggactatcc
     8401 agcagagaaa gtgggaggcg tgctgaaccc ataccagctg acagaccagt tcacctcctt
     8461 tgccaagatg ggcacccagt ctggcttcct gttttacgtg cctgccccat atacatctaa
     8521 gatcgatccc ctgaccggct tcgtggaccc cttcgtgtgg aaaaccatca agaatcacga
     8581 gagccgcaag cacttcctgg agggcttcga ctttctgcac tacgacgtga aaaccggcga
     8641 cttcatcctg cactttaaga tgaacagaaa tctgtccttc cagaggggcc tgcccggctt
     8701 tatgcctgca tgggatatcg tgttcgagaa gaacgagaca cagtttgacg ccaagggcac
     8761 ccctttcatc gccggcaaga gaatcgtgcc agtgatcgag aatcacagat tcaccggcag
     8821 ataccgggac ctgtatcctg ccaacgagct gatcgccctg ctggaggaga agggcatcgt
     8881 gttcagggat ggctccaaca tcctgccaaa gctgctggag aatgacgatt ctcacgccat
     8941 cgacaccatg gtggccctga tccgcagcgt gctgcagatg cggaactcca atgccgccac
     9001 aggcgaggac tatatcaaca gccccgtgcg cgatctgaat ggcgtgtgct tcgactcccg
     9061 gtttcagaac ccagagtggc ccatggacgc cgatgccaat ggcgcctacc acatcgccct
     9121 gaagggccag ctgctgctga atcacctgaa ggagagcaag gatctgaagc tgcagaacgg
     9181 catctccaat caggactggc tggcctacat ccaggagctg cgcaacaaaa ggccggcggc
     9241 cacgaaaaag gccggccagg caaaaaagaa aaagggcagc agcggatcca gcgagctgat
     9301 taaggagaac atgcacatga agctgtacat ggagggcacc gtggacaacc atcacttcaa
     9361 gtgcacatcc gagggcgaag gcaagcccta cgagggcacc cagaccatga gaatcaaggt
     9421 ggtcgagggc ggccctctcc ccttcgcctt cgacatcctg gctactagct tcctctacgg
     9481 cagcaagacc ttcatcaacc acacccaggg catccccgac ttcttcaagc agtccttccc
     9541 tgagggcttc acatgggaga gagtcaccac atacgaagac gggggcgtgc tgaccgctac
     9601 ccaggacacc agcctccagg acggctgcct catctacaac gtcaagatca gaggggtgaa
     9661 cttcacatcc aacggccctg tgatgcagaa gaaaacactc ggctgggagg ccttcaccga
     9721 gacgctgtac cccgctgacg gcggcctgga aggcagaaac gacatggccc tgaagctcgt
     9781 gggcgggagc catctgatcg caaacatcaa gaccacatat agatccaaga aacccgctaa
     9841 gaacctcaag atgcctggcg tctactatgt ggactacaga ctggaaagaa tcaaggaggc
     9901 caacaacgag acctacgtcg agcagcacga ggtggcagtg gccagatact gcgacctccc
     9961 tagcaaactg gggcacaagc ttaattagta actagcttga ctgactgaga tacagcgtac
    10021 cttcagctca cagacatgat aagatacatt gatgagtttg gacaaaccac aactagaatg
    10081 cagtgaaaaa aatgctttat ttgtgaaatt tgtgatgcta ttgctttatt tgtaaccatt
    10141 ataagctgca ataaacaagt taacaacaac aattgcattc attttatgtt tcaggttcag
    10201 ggggaggtgt gggaggtttt ttaaagcaag taaaacctct acaaatgtgg tattggccca
    10261 tctctatcgg tatcgtagca taaccccttg gggcctctaa acgggtcttg aggggttttt
    10321 tgtgcccctc gggccggatt gctatctacc ggcattggcg cagaaaaaaa tgcctgatgc
    10381 gacgctgcgc gtcttatact cccacatatg ccagattcag caacggatac ggcttcccca
    10441 acttgcccac ttccatacgt gtcctcctta ccagaaattt atccttaagg tcgtcagcta
    10501 tcctgcaggc gatctctcga tttcgatcaa gacattcctt taatggtctt ttctggacac
    10561 cactaggggt cagaagtagt tcatcaaact ttcttccctc cctaatctca ttggttacct
    10621 tgggctatcg aaacttaatt aaccagtcaa gtcagctact tggcgagatc gacttgtctg
    10681 ggtttcgact acgctcagaa ttgcgtcagt caagttcgat ctggtccttg ctattgcacc
    10741 cgttctccga ttacgagttt catttaaatc atgtgagcaa aaggccagca aaaggccagg
    10801 aaccgtaaaa aggccgcgtt gctggcgttt ttccataggc tccgcccccc tgacgagcat
    10861 cacaaaaatc gacgctcaag tcagaggtgg cgaaacccga caggactata aagataccag
    10921 gcgtttcccc ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga
    10981 tacctgtccg cctttctccc ttcgggaagc gtggcgcttt ctcatagctc acgctgtagg
    11041 tatctcagtt cggtgtaggt cgttcgctcc aagctgggct gtgtgcacga accccccgtt
    11101 cagcccgacc gctgcgcctt atccggtaac tatcgtcttg agtccaaccc ggtaagacac
    11161 gacttatcgc cactggcagc agccactggt aacaggatta gcagagcgag gtatgtaggc
    11221 ggtgctacag agttcttgaa gtggtggcct aactacggct acactagaag aacagtattt
    11281 ggtatctgcg ctctgctgaa gccagttacc ttcggaaaaa gagttggtag ctcttgatcc
    11341 ggcaaacaaa ccaccgctgg tagcggtggt ttttttgttt gcaagcagca gattacgcgc
    11401 agaaaaaaag gatctcaa
//
