LOCUS       sequence-496451-       11779 bp    DNA     circular SYN 15-JAN-2026
DEFINITION  Stable transformation or transient expression of gene of interest in
            plants.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11779)
  AUTHORS   Wan WL, Kim G, Kim N, Tan YY, Watari M, Charoennit N, Lee RRQ, Phang
            IRK, Wang J, Liew YY, Hu D, Ng SK, Zhang Y, Jang IC, Song JJ, Chae 
            E.
  TITLE     Structural determinants of DANGEROUS MIX 3, an alpha/beta hydrolase 
            that triggers NLR-mediated genetic incompatibility in plants.
  JOURNAL   Molecular Cell, Volume 85, Issue 14
REFERENCE   2  (bases 1 to 11779)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Jan 15, 2026 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Molecular 
            Cell, Volume 85, Issue 14"
COMMENT     Sequence Label: pGWB414_35S-DM3(Hh-0)-HA
FEATURES             Location/Qualifiers
     source          1..11779
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    54..78
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     primer_bind     266..288
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     280..297
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     281..297
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        812..1157
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     primer_bind     1100..1120
                     /label=35S promoter
                     /note="CaMV 35S promoter, forward primer"
     protein_bind    1179..1203
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(3542..3566)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             3595..3684
                     /codon_start=1
                     /product="three tandem HA epitope tags"
                     /label=3xHA
                     /translation="YPYDVPDYAGYPYDVPDYAGSYPYDVPDYA"
     CDS             complement(4097..4891)
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGDDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(4208..4227)
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     4818..4837
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     promoter        complement(4912..5095)
                     /function="NOS promoter"
                     /label=NOS promoter
                     /note="nopaline synthase promoter"
     promoter        complement(4948..5127)
                     /label=NOS promoter
                     /note="nopaline synthase promoter"
     misc_feature    5301..5325
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     CDS             5846..6637
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MGEAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      6883..7471
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     7372..7391
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     7625..7642
                     /label=L4440
                     /note="L4440 vector, forward primer"
     misc_feature    7657..7797
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     complement(7723..7742)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     7883..7905
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     rep_origin      8141..8335
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     CDS             complement(8401..9474)
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="MSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
                     QAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
                     KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
                     GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
                     ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
                     LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
                     LVMRYRNLIEGEASAGS"
     CDS             complement(9903..10532)
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
ORIGIN
        1 tttcacgccc ttttaaatat ccgattattc taataaacgc tcttttctct taggtttacc
       61 cgccaatata tcctgtcaaa cactgatagt ttaaactgaa ggcgggaaac gacaatctga
      121 tccaagctca agctgctcta gcattcgcca ttcaggctgc gcaactgttg ggaagggcga
      181 tcggtgcggg cctcttcgct attacgccag ctggcgaaag ggggatgtgc tgcaaggcga
      241 ttaagttggg taacgccagg gttttcccag tcacgacgtt gtaaaacgac ggccagtgcc
      301 aagcttgcat gcctgcaggt ccccagatta gccttttcaa tttcagaaag aatgctaacc
      361 cacagatggt tagagaggct tacgcagcag gtctcatcaa gacgatctac ccgagcaata
      421 atctccagga aatcaaatac cttcccaaga aggttaaaga tgcagtcaaa agattcagga
      481 ctaactgcat caagaacaca gagaaagata tatttctcaa gatcagaagt actattccag
      541 tatggacgat tcaaggcttg cttcacaaac caaggcaagt aatagagatt ggagtctcta
      601 aaaaggtagt tcccactgaa tcaaaggcca tggagtcaaa gattcaaata gaggacctaa
      661 cagaactcgc cgtaaagact ggcgaacagt tcatacagag tctcttacga ctcaatgaca
      721 agaagaaaat cttcgtcaac atggtggagc acgacacact tgtctactcc aaaaatatca
      781 aagatacagt ctcagaagac caaagggcaa ttgagacttt tcaacaaagg gtaatatccg
      841 gaaacctcct cggattccat tgcccagcta tctgtcactt tattgtgaag atagtggaaa
      901 aggaaggtgg ctcctacaaa tgccatcatt gcgataaagg aaaggccatc gttgaagatg
      961 cctctgccga cagtggtccc aaagatggac ccccacccac gaggagcatc gtggaaaaag
     1021 aagacgttcc aaccacgtct tcaaagcaag tggattgatg tgatatctcc actgacgtaa
     1081 gggatgacgc acaatcccac tatccttcgc aagacccttc ctctatataa ggaagttcat
     1141 ttcatttgga gagaacacgg gggactctag agttatcaac aagtttgtac aaaaaagcag
     1201 gctccgaatt cgcccttaag aataaaaacg atgttggcga ctcacgcgct ggtgactccg
     1261 attccaaagt tgactgcttt cgtactctct ctatctacta ctccatcaag attgtctggt
     1321 tctgttccgg gtttgattga tttctaccgt cgccggagat tttgtcgtgt aatcacctcc
     1381 atggctgaag cgggatcggt ttacgtcgat gtagctgggg aatcgaagtc ggagcatgtt
     1441 actgggaagt ggttttcggt gccggagctc cgactgcgtg accaccggtt catagttcct
     1501 cttgattatt ccaaaagttc tcccaaaatt accgtctttg ctcgagagat cgttgcgggt
     1561 aacttgaaat ttggagcttt ttgattcaga gctctttctg tgattacgaa gctaaaagtt
     1621 tgtgtctttt gttttttgta gttgggaaag aggagcaagc aatgccgtat ttattgtatt
     1681 tgcaaggtgg tccaggattt gaaggtccaa ggcctagtga agctagtgga tggattcaaa
     1741 gagcttgtga agagtttcga gtggtcttac tcgatcaagt atgatgcttt catagtttta
     1801 tactcttctc tggacagttt ttttagtctt tttgttgttc taattttgtt tgtcctgcag
     1861 cgaggaacag gcttgtccac tcctttaata tgttcctcta tgcttcaatt caaatcagca
     1921 aaggaattag ctgattattt ggttcacttt cgagctgata atatagttaa agatgctgag
     1981 tttattagag ttcgtcttgt tcctaaggct gatccttgga caattttggg tcaggtaatt
     2041 tgtctttgta ttgtctattg gtcatcttgc ttcagttctt gtcatttgat tttgtattca
     2101 tctttttttc ttcaaatcta cagagtttcg gtggcttttg tgcacttaca tatctgagtt
     2161 ttgcacccga aggactcaaa caagtgctaa taactggagg aattccacca attggtaaag
     2221 catgtacagc agatgatgta tatgaagccg gttttgaaca agttgctcgc caaaatgaaa
     2281 aatactacaa gagattccct caagacattg agattgttcg tgaactcgta aattacttgg
     2341 ctgaatctga aggtggcggg gtgagggatt acaacatttg ctcaatccac tgaccagttt
     2401 agtttagttc cttacttgtc tctgtttctt tgtctttagg tacctctccc atcaggaggc
     2461 attcttactc ctaagggtct tcaaactctt ggtctttctg gtttaggatc aagtactggt
     2521 tttgagcgcc tgcattatat gtaagagttt agcttaattt ttatcaacca cctcacatgt
     2581 tctgtgttaa atctggcctt cttaattgat cataggttgg agagagtatg ggatcctatt
     2641 ttagttactg gagctccaaa gtgtattagt cagttcttct taaacgctgt aagagaccca
     2701 caaaaatttg ttgttttctg ttctcagctc aaataaggaa ttcaatagtc tgagacattt
     2761 tatcgttgct gcagtttgag agttggcatt cttttgatgc aaatccacta tatgctcttc
     2821 tccacgaggc catatactgt gaggtatagt ctcttcaaat ttctcatctt tttttctatt
     2881 tcagacaatt ccttcttctt gttatcattt atgtttaaat ggaaactttt tcagggtgct
     2941 tcatcaggat ggtctgctca tagattacgg gataaatatg agtacaaatt cgacgcaatg
     3001 aaggcagtca aagaaagtca gcctgtactc ttcacaggag aggtacaggt tttgagtatt
     3061 tctaattgta tggctcatag cttggatgat tggtccacat tgcttaacta aaatagaatt
     3121 tggatttgta tagatgatat ttccatggat gtttgatgaa attcatgcct tgaagccatt
     3181 taaagctgcc gccgatcttt tggccaagaa agaggattgg cctccattat atgatgttcc
     3241 tagattgcag aataacaagg tctgtttcag aatcttgatc gttctttcat atggatcaaa
     3301 taattttact ctgtttcaca tgatttcttt ttgtaggtgc ctgttgctgc agcggtttac
     3361 tacgaagata tgtatgtaaa cttcaagctg gtgacggaaa cagcttctca tatatcgggt
     3421 atcagacttt gggtaacgaa tgagtttatg cattcgggtc ttcgtgatgc tggaagacaa
     3481 attattgatc atttgttggg aatgatcaat gggaaaaagc ctctctttaa gggcgaattc
     3541 gacccagctt tcttgtacaa agtggttgat aacagcgggt taattaacat cttttaccca
     3601 tacgatgttc ctgactatgc gggctatccc tatgacgtcc cggactatgc aggatcctat
     3661 ccatatgacg ttccagatta cgctgctcag tgcagcgctt agagctcgaa tttcatccgg
     3721 gtctgtggcg ggaactccac gaaaatatcc gaacgcagca agatatcgcg gtgcatctcg
     3781 gtcttgcctg ggcagtcgcc gccgacgccg ttgatgtgga cgccgggccc gatcatattg
     3841 tcgctcagga tcgtggcgtt gtgcttgtcg gccgttgctg tcgtaatgat atcggcacct
     3901 tcgaccgcct gttccgcaga gatcccgtgg gcgaagaact ccagcatgag atccccgcgc
     3961 tggaggatca tccagccggc gtcccggaaa acgattccga agcccaacct ttcatagaag
     4021 gcggcggtgg aatcgaaatc tcgtgatggc aggttgggcg tcgcttggtc ggtcatttcg
     4081 aaccccagag tcccgctcag aagaactcgt caagaaggcg atagaaggcg atgcgctgcg
     4141 aatcgggagc ggcgataccg taaagcacga ggaagcggtc agcccattcg ccgccaagct
     4201 cttcagcaat atcacgggta gccaacgcta tgtcctgata gcggtccgcc acacccagcc
     4261 ggccacagtc gatgaatcca gaaaagcggc cattttccac catgatattc ggcaagcagg
     4321 catcgccatg ggtcacgacg agatcatcgc cgtcgggcat gcgcgccttg agcctggcga
     4381 acagttcggc tggcgcgagc ccctgatgct cttcgtccag atcatcctga tcgacaagac
     4441 cggcttccat ccgagtacgt gctcgctcga tgcgatgttt cgcttggtgg tcgaatgggc
     4501 aggtagccgg atcaagcgta tgcagccgcc gcattgcatc agccatgatg gatactttct
     4561 cggcaggagc aaggtgagat gacaggagat cctgccccgg cacttcgccc aatagcagcc
     4621 agtcccttcc cgcttcagtg acaacgtcga gcacagctgc gcaaggaacg cccgtcgtgg
     4681 ccagccacga tagccgcgct gcctcgtcct gcagttcatt cagggcaccg gacaggtcgg
     4741 tcttgacaaa aagaaccggg cgcccctgcg ctgacagccg gaacacggcg gcatcagagc
     4801 agccgattgt ctgttgtgcc cagtcatagc cgaatagcct ctccacccaa gcggccggag
     4861 aacctgcgtg caatccatct tgttcaatca tgcgaaacga tccagatccg gtgcagatta
     4921 tttggattga gagtgaatat gagactctaa ttggataccg aggggaattt atggaacgtc
     4981 agtggagcat ttttgacaag aaatatttgc tagctgatag tgaccttagg cgacttttga
     5041 acgcgcaata atggtttctg acgtatgtgc ttagctcatt aaactccaga aacccgcggc
     5101 tgagtggctc cttcaacgtt gcggttctgt cagttccaaa cgtaaaacgg cttgtcccgc
     5161 gtcatcggcg ggggtcataa cgtgactccc ttaattctcc gctcatgatg aattcagaaa
     5221 taaattcagc ctaattcggc gttaattcag tacattaaaa acgtccgcaa tgtgttatta
     5281 agttgtctaa gcgtcaattt gtttacacca caatatatcc tgccaccagc cagccaacag
     5341 ctccccgacc ggcagctcgg cacaaaatca ccactcgata caggcagccc atcagtccgg
     5401 gacggcgtca gcgggagagc cgttgtaagg cggcagactt tgctcatgtt accgatgcta
     5461 ttcggaagaa cggcaactaa gctgccgggt ttgaaacacg gatgatctcg cggagggtag
     5521 catgttgatt gtaacgatga cagagcgttg ctgcctgtga tcaattcggg cacgaaccca
     5581 gtggacataa gcctgttcgg ttcgtaagct gtaatgcaag tagcgtatgc gctcacgcaa
     5641 ctggtccaga accttgaccg aacgcagcgg tggtaacggc gcagtggcgg ttttcatggc
     5701 ttgttatgac tgtttttttg gggtacagtc tatgcctcgg gcatccaagc agcaagcgcg
     5761 ttacgccgtg ggtcgatgtt tgatgttatg gagcagcaac gatgttacgc agcagggcag
     5821 tcgccctaaa acaaagttaa acatcatggg ggaagcggtg atcgccgaag tatcgactca
     5881 actatcagag gtagttggcg tcatcgagcg ccatctcgaa ccgacgttgc tggccgtaca
     5941 tttgtacggc tccgcagtgg atggcggcct gaagccacac agtgatattg atttgctggt
     6001 tacggtgacc gtaaggcttg atgaaacaac gcggcgagct ttgatcaacg accttttgga
     6061 aacttcggct tcccctggag agagcgagat tctccgcgct gtagaagtca ccattgttgt
     6121 gcacgacgac atcattccgt ggcgttatcc agctaagcgc gaactgcaat ttggagaatg
     6181 gcagcgcaat gacattcttg caggtatctt cgagccagcc acgatcgaca ttgatctggc
     6241 tatcttgctg acaaaagcaa gagaacatag cgttgccttg gtaggtccag cggcggagga
     6301 actctttgat ccggttcctg aacaggatct atttgaggcg ctaaatgaaa ccttaacgct
     6361 atggaactcg ccgcccgact gggctggcga tgagcgaaat gtagtgctta cgttgtcccg
     6421 catttggtac agcgcagtaa ccggcaaaat cgcgccgaag gatgtcgctg ccgactgggc
     6481 aatggagcgc ctgccggccc agtatcagcc cgtcatactt gaagctagac aggcttatct
     6541 tggacaagaa gaagatcgct tggcctcgcg cgcagatcag ttggaagaat ttgtccacta
     6601 cgtgaaaggc gagatcacca aggtagtcgg caaataatgt ctagctagaa attcgttcaa
     6661 gccgacgccg cttcgcggcg cggcttaact caagcgttag atgcactaag cacataattg
     6721 ctcacagcca aactatcagg tcaagtctgc ttttattatt tttaagcgtg cataataagc
     6781 cctacacaaa ttgggagata tatcatgcat gaccaaaatc ccttaacgtg agttttcgtt
     6841 ccactgagcg tcagaccccg tagaaaagat caaaggatct tcttgagatc ctttttttct
     6901 gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc
     6961 ggatcaagag ctaccaactc tttttccgaa ggtaactggc ttcagcagag cgcagatacc
     7021 aaatactgtc cttctagtgt agccgtagtt aggccaccac ttcaagaact ctgtagcacc
     7081 gcctacatac ctcgctctgc taatcctgtt accagtggct gctgccagtg gcgataagtc
     7141 gtgtcttacc gggttggact caagacgata gttaccggat aaggcgcagc ggtcgggctg
     7201 aacggggggt tcgtgcacac agcccagctt ggagcgaacg acctacaccg aactgagata
     7261 cctacagcgt gagctatgag aaagcgccac gcttcccgaa gggagaaagg cggacaggta
     7321 tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg gagcttccag ggggaaacgc
     7381 ctggtatctt tatagtcctg tcgggtttcg ccacctctga cttgagcgtc gatttttgtg
     7441 atgctcgtca ggggggcgga gcctatggaa aaacgccagc aacgcggcct ttttacggtt
     7501 cctggccttt tgctggcctt ttgctcacat gttctttcct gcgttatccc ctgattctgt
     7561 ggataaccgt attaccgcct ttgagtgagc tgataccgct cgccgcagcc gaacgaccga
     7621 gcgcagcgag tcagtgagcg aggaagcgga agagcgcctg atgcggtatt ttctccttac
     7681 gcatctgtgc ggtatttcac accgcatatg gtgcactctc agtacaatct gctctgatgc
     7741 cgcatagtta agccagtata cactccgcta tcgctacgtg actgggtcat ggctgcgccc
     7801 cgacacccgc caacacccgc tgacgcgccc tgacgggctt gtctgctccc ggcatccgct
     7861 tacagacaag ctgtgaccgt ctccgggagc tgcatgtgtc agaggttttc accgtcatca
     7921 ccgaaacgcg cgaggcaggg tgccttgatg tgggcgccgg cggtcgagtg gcgacggcgc
     7981 ggcttgtccg cgccctggta gattgcctgg ccgtaggcca gccatttttg agcggccagc
     8041 ggccgcgata ggccgacgcg aagcggcggg gcgtagggag cgcagcgacc gaagggtagg
     8101 cgctttttgc agctcttcgg ctgtgcgctg gccagacagt tatgcacagg ccaggcgggt
     8161 tttaagagtt ttaataagtt ttaaagagtt ttaggcggaa aaatcgcctt ttttctcttt
     8221 tatatcagtc acttacatgt gtgaccggtt cccaatgtac ggctttgggt tcccaatgta
     8281 cgggttccgg ttcccaatgt acggctttgg gttcccaatg tacgtgctat ccacaggaaa
     8341 gagacctttt cgaccttttt cccctgctag ggcaatttgc cctagcatct gctccgtaca
     8401 ttaggaaccg gcggatgctt cgccctcgat caggttgcgg tagcgcatga ctaggatcgg
     8461 gccagcctgc cccgcctcct ccttcaaatc gtactccggc aggtcatttg acccgatcag
     8521 cttgcgcacg gtgaaacaga acttcttgaa ctctccggcg ctgccactgc gttcgtagat
     8581 cgtcttgaac aaccatctgg cttctgcctt gcctgcggcg cggcgtgcca ggcggtagag
     8641 aaaacggccg atgccgggat cgatcaaaaa gtaatcgggg tgaaccgtca gcacgtccgg
     8701 gttcttgcct tctgtgatct cgcggtacat ccaatcagct agctcgatct cgatgtactc
     8761 cggccgcccg gtttcgctct ttacgatctt gtagcggcta atcaaggctt caccctcgga
     8821 taccgtcacc aggcggccgt tcttggcctt cttcgtacgc tgcatggcaa cgtgcgtggt
     8881 gtttaaccga atgcaggttt ctaccaggtc gtctttctgc tttccgccat cggctcgccg
     8941 gcagaacttg agtacgtccg caacgtgtgg acggaacacg cggccgggct tgtctccctt
     9001 cccttcccgg tatcggttca tggattcggt tagatgggaa accgccatca gtaccaggtc
     9061 gtaatcccac acactggcca tgccggccgg ccctgcggaa acctctacgt gcccgtctgg
     9121 aagctcgtag cggatcacct cgccagctcg tcggtcacgc ttcgacagac ggaaaacggc
     9181 cacgtccatg atgctgcgac tatcgcgggt gcccacgtca tagagcatcg gaacgaaaaa
     9241 atctggttgc tcgtcgccct tgggcggctt cctaatcgac ggcgcaccgg ctgccggcgg
     9301 ttgccgggat tctttgcgga ttcgatcagc ggccgcttgc cacgattcac cggggcgtgc
     9361 ttctgcctcg atgcgttgcc gctgggcggc ctgcgcggcc ttcaacttct ccaccaggtc
     9421 atcacccagc gccgcgccga tttgtaccgg gccggatggt ttgcgaccgc tcacgccgat
     9481 tcctcgggct tgggggttcc agtgccattg cagggccggc agacaaccca gccgcttacg
     9541 cctggccaac cgcccgttcc tccacacatg gggcattcca cggcgtcggt gcctggttgt
     9601 tcttgatttt ccatgccgcc tcctttagcc gctaaaattc atctactcat ttattcattt
     9661 gctcatttac tctggtagct gcgcgatgta ttcagatagc agctcggtaa tggtcttgcc
     9721 ttggcgtacc gcgtacatct tcagcttggt gtgatcctcc gccggcaact gaaagttgac
     9781 ccgcttcatg gctggcgtgt ctgccaggct ggccaacgtt gcagccttgc tgctgcgtgc
     9841 gctcggacgg ccggcactta gcgtgtttgt gcttttgctc attttctctt tacctcatta
     9901 actcaaatga gttttgattt aatttcagcg gccagcgcct ggacctcgcg ggcagcgtcg
     9961 ccctcgggtt ctgattcaag aacggttgtg ccggcggcgg cagtgcctgg gtagctcacg
    10021 cgctgcgtga tacgggactc aagaatgggc agctcgtacc cggccagcgc ctcggcaacc
    10081 tcaccgccga tgcgcgtgcc tttgatcgcc cgcgacacga caaaggccgc ttgtagcctt
    10141 ccatccgtga cctcaatgcg ctgcttaacc agctccacca ggtcggcggt ggcccatatg
    10201 tcgtaagggc ttggctgcac cggaatcagc acgaagtcgg ctgccttgat cgcggacaca
    10261 gccaagtccg ccgcctgggg cgctccgtcg atcactacga agtcgcgccg gccgatggcc
    10321 ttcacgtcgc ggtcaatcgt cgggcggtcg atgccgacaa cggttagcgg ttgatcttcc
    10381 cgcacggccg cccaatcgcg ggcactgccc tggggatcgg aatcgactaa cagaacatcg
    10441 gccccggcga gttgcagggc gcgggctaga tgggttgcga tggtcgtctt gcctgacccg
    10501 cctttctggt taagtacagc gataaccttc atgcgttccc cttgcgtatt tgtttattta
    10561 ctcatcgcat catatacgca gcgaccgcat gacgcaagct gttttactca aatacacatc
    10621 acctttttag acggcggcgc tcggtttctt cagcggccaa gctggccggc caggccgcca
    10681 gcttggcatc agacaaaccg gccaggattt catgcagccg cacggttgag acgtgcgcgg
    10741 gcggctcgaa cacgtacccg gccgcgatca tctccgcctc gatctcttcg gtaatgaaaa
    10801 acggttcgtc ctggccgtcc tggtgcggtt tcatgcttgt tcctcttggc gttcattctc
    10861 ggcggccgcc agggcgtcgg cctcggtcaa tgcgtcctca cggaaggcac cgcgccgcct
    10921 ggcctcggtg ggcgtcactt cctcgctgcg ctcaagtgcg cggtacaggg tcgagcgatg
    10981 cacgccaagc agtgcagccg cctctttcac ggtgcggcct tcctggtcga tcagctcgcg
    11041 ggcgtgcgcg atctgtgccg gggtgagggt agggcggggg ccaaacttca cgcctcgggc
    11101 cttggcggcc tcgcgcccgc tccgggtgcg gtcgatgatt agggaacgct cgaactcggc
    11161 aatgccggcg aacacggtca acaccatgcg gccggccggc gtggtggtgt cggcccacgg
    11221 ctctgccagg ctacgcaggc ccgcgccggc ctcctggatg cgctcggcaa tgtccagtag
    11281 gtcgcgggtg ctgcgggcca ggcggtctag cctggtcact gtcacaacgt cgccagggcg
    11341 taggtggtca agcatcctgg ccagctccgg gcggtcgcgc ctggtgccgg tgatcttctc
    11401 ggaaaacagc ttggtgcagc cggccgcgtg cagttcggcc cgttggttgg tcaagtcctg
    11461 gtcgtcggtg ctgacgcggg catagcccag caggccagcg gcggcgctct tgttcatggc
    11521 gtaatgtctc cggttctagt cgcaagtatt ctactttatg cgactaaaac acgcgacaag
    11581 aaaacgccag gaaaagggca gggcggcagc ctgtcgcgta acttaggact tgtgcgacat
    11641 gtcgttttca gaagacggct gcactgaacg tcagaagccg actgcactat agcagcggag
    11701 gggttggatc aaagtacttt gatcccgagg ggaaccctgt ggttggcatg cacatacaaa
    11761 tggacgaacg gataaacct
//
