LOCUS       sequence-496470-       12309 bp    DNA     circular SYN 15-JAN-2026
DEFINITION  Stable transformation or transient expression of gene of interest in
            plants.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12309)
  AUTHORS   Wan WL, Kim G, Kim N, Tan YY, Watari M, Charoennit N, Lee RRQ, Phang
            IRK, Wang J, Liew YY, Hu D, Ng SK, Zhang Y, Jang IC, Song JJ, Chae 
            E.
  TITLE     Structural determinants of DANGEROUS MIX 3, an alpha/beta hydrolase 
            that triggers NLR-mediated genetic incompatibility in plants.
  JOURNAL   Molecular Cell, Volume 85, Issue 14
REFERENCE   2  (bases 1 to 12309)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Jan 15, 2026 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Molecular 
            Cell, Volume 85, Issue 14"
COMMENT     Sequence Label: pGWB414_35S-(Delta-TP)DM3(Hh-0)-HA
FEATURES             Location/Qualifiers
     source          1..12309
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    54..78
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     primer_bind     266..288
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     280..297
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     281..297
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        812..1157
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     primer_bind     1100..1120
                     /label=35S promoter
                     /note="CaMV 35S promoter, forward primer"
     protein_bind    1179..1203
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(3376..3400)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             3429..3518
                     /codon_start=1
                     /product="three tandem HA epitope tags"
                     /label=3xHA
                     /translation="YPYDVPDYAGYPYDVPDYAGSYPYDVPDYA"
     terminator      3552..3804
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     primer_bind     complement(3837..3853)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3837..3853)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(3850..3872)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    3861..3877
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(3885..3891,3892..3909,3910..3915))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    3930..3951
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     terminator      complement(3985..4237)
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     CDS             complement(4627..5421)
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGDDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(4738..4757)
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     5348..5367
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     promoter        complement(5442..5625)
                     /function="NOS promoter"
                     /label=NOS promoter
                     /note="nopaline synthase promoter"
     promoter        complement(5478..5657)
                     /label=NOS promoter
                     /note="nopaline synthase promoter"
     misc_feature    5831..5855
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     CDS             6376..7167
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MGEAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      7413..8001
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     7902..7921
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     8155..8172
                     /label=L4440
                     /note="L4440 vector, forward primer"
     misc_feature    8187..8327
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     complement(8253..8272)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     8413..8435
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     rep_origin      8671..8865
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     CDS             complement(8931..10004)
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="MSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
                     QAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
                     KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
                     GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
                     ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
                     LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
                     LVMRYRNLIEGEASAGS"
     CDS             complement(10433..11062)
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
ORIGIN
        1 tttcacgccc ttttaaatat ccgattattc taataaacgc tcttttctct taggtttacc
       61 cgccaatata tcctgtcaaa cactgatagt ttaaactgaa ggcgggaaac gacaatctga
      121 tccaagctca agctgctcta gcattcgcca ttcaggctgc gcaactgttg ggaagggcga
      181 tcggtgcggg cctcttcgct attacgccag ctggcgaaag ggggatgtgc tgcaaggcga
      241 ttaagttggg taacgccagg gttttcccag tcacgacgtt gtaaaacgac ggccagtgcc
      301 aagcttgcat gcctgcaggt ccccagatta gccttttcaa tttcagaaag aatgctaacc
      361 cacagatggt tagagaggct tacgcagcag gtctcatcaa gacgatctac ccgagcaata
      421 atctccagga aatcaaatac cttcccaaga aggttaaaga tgcagtcaaa agattcagga
      481 ctaactgcat caagaacaca gagaaagata tatttctcaa gatcagaagt actattccag
      541 tatggacgat tcaaggcttg cttcacaaac caaggcaagt aatagagatt ggagtctcta
      601 aaaaggtagt tcccactgaa tcaaaggcca tggagtcaaa gattcaaata gaggacctaa
      661 cagaactcgc cgtaaagact ggcgaacagt tcatacagag tctcttacga ctcaatgaca
      721 agaagaaaat cttcgtcaac atggtggagc acgacacact tgtctactcc aaaaatatca
      781 aagatacagt ctcagaagac caaagggcaa ttgagacttt tcaacaaagg gtaatatccg
      841 gaaacctcct cggattccat tgcccagcta tctgtcactt tattgtgaag atagtggaaa
      901 aggaaggtgg ctcctacaaa tgccatcatt gcgataaagg aaaggccatc gttgaagatg
      961 cctctgccga cagtggtccc aaagatggac ccccacccac gaggagcatc gtggaaaaag
     1021 aagacgttcc aaccacgtct tcaaagcaag tggattgatg tgatatctcc actgacgtaa
     1081 gggatgacgc acaatcccac tatccttcgc aagacccttc ctctatataa ggaagttcat
     1141 ttcatttgga gagaacacgg gggactctag agttatcaac aagtttgtac aaaaaagcag
     1201 gctccgaatt cgcccttatg gaagcgggat cggtttacgt cgatgtagct ggggaatcga
     1261 agtcggagca tgttactggg aagtggtttt cggtgccgga gctccgactg cgtgaccacc
     1321 ggttcatagt tcctcttgat tattccaaaa gttctcccaa aattaccgtc tttgctcgag
     1381 agatcgttgc gggtaacttg aaatttggag ctttttgatt cagagctctt tctgtgatta
     1441 cgaagctaaa agtttgtgtc ttttgttttt tgtagttggg aaagaggagc aagcaatgcc
     1501 gtatttattg tatttgcaag gtggtccagg atttgaaggt ccaaggccta gtgaagctag
     1561 tggatggatt caaagagctt gtgaagagtt tcgagtggtc ttactcgatc aagtatgatg
     1621 ctttcatagt tttatactct tctctggaca gtttttttag tctttttgtt gttctaattt
     1681 tgtttgtcct gcagcgagga acaggcttgt ccactccttt aatatgttcc tctatgcttc
     1741 aattcaaatc agcaaaggaa ttagctgatt atttggttca ctttcgagct gataatatag
     1801 ttaaagatgc tgagtttatt agagttcgtc ttgttcctaa ggctgatcct tggacaattt
     1861 tgggtcaggt aatttgtctt tgtattgtct attggtcatc ttgcttcagt tcttgtcatt
     1921 tgattttgta ttcatctttt tttcttcaaa tctacagagt ttcggtggct tttgtgcact
     1981 tacatatctg agttttgcac ccgaaggact caaacaagtg ctaataactg gaggaattcc
     2041 accaattggt aaagcatgta cagcagatga tgtatatgaa gccggttttg aacaagttgc
     2101 tcgccaaaat gaaaaatact acaagagatt ccctcaagac attgagattg ttcgtgaact
     2161 cgtaaattac ttggctgaat ctgaaggtgg cggggtgagg gattacaaca tttgctcaat
     2221 ccactgacca gtttagttta gttccttact tgtctctgtt tctttgtctt taggtacctc
     2281 tcccatcagg aggcattctt actcctaagg gtcttcaaac tcttggtctt tctggtttag
     2341 gatcaagtac tggttttgag cgcctgcatt atatgtaaga gtttagctta atttttatca
     2401 accacctcac atgttctgtg ttaaatctgg ccttcttaat tgatcatagg ttggagagag
     2461 tatgggatcc tattttagtt actggagctc caaagtgtat tagtcagttc ttcttaaacg
     2521 ctgtaagaga cccacaaaaa tttgttgttt tctgttctca gctcaaataa ggaattcaat
     2581 agtctgagac attttatcgt tgctgcagtt tgagagttgg cattcttttg atgcaaatcc
     2641 actatatgct cttctccacg aggccatata ctgtgaggta tagtctcttc aaatttctca
     2701 tctttttttc tatttcagac aattccttct tcttgttatc atttatgttt aaatggaaac
     2761 tttttcaggg tgcttcatca ggatggtctg ctcatagatt acgggataaa tatgagtaca
     2821 aattcgacgc aatgaaggca gtcaaagaaa gtcagcctgt actcttcaca ggagaggtac
     2881 aggttttgag tatttctaat tgtatggctc atagcttgga tgattggtcc acattgctta
     2941 actaaaatag aatttggatt tgtatagatg atatttccat ggatgtttga tgaaattcat
     3001 gccttgaagc catttaaagc tgccgccgat cttttggcca agaaagagga ttggcctcca
     3061 ttatatgatg ttcctagatt gcagaataac aaggtctgtt tcagaatctt gatcgttctt
     3121 tcatatggat caaataattt tactctgttt cacatgattt ctttttgtag gtgcctgttg
     3181 ctgcagcggt ttactacgaa gatatgtatg taaacttcaa gctggtgacg gaaacagctt
     3241 ctcatatatc gggtatcaga ctttgggtaa cgaatgagtt tatgcattcg ggtcttcgtg
     3301 atgctggaag acaaattatt gatcatttgt tgggaatgat caatgggaaa aagcctctct
     3361 ttaagggcga attcgaccca gctttcttgt acaaagtggt tgataacagc gggttaatta
     3421 acatctttta cccatacgat gttcctgact atgcgggcta tccctatgac gtcccggact
     3481 atgcaggatc ctatccatat gacgttccag attacgctgc tcagtgcagc gcttagagct
     3541 cgaatttccc cgatcgttca aacatttggc aataaagttt cttaagattg aatcctgttg
     3601 ccggtcttgc gatgattatc atataatttc tgttgaatta cgttaagcat gtaataatta
     3661 acatgtaatg catgacgtta tttatgagat gggtttttat gattagagtc ccgcaattat
     3721 acatttaata cgcgatagaa aacaaaatat agcgcgcaaa ctaggataaa ttatcgcgcg
     3781 cggtgtcatc tatgttacta gatcgggaat tggttccgga accaattcgt aatcatgtca
     3841 tagctgtttc ctgtgtgaaa ttgttatccg ctcacaattc cacacaacat acgagccgga
     3901 agcataaagt gtaaagcctg gggtgcctaa tgagtgagct aactcacatt aggctgaatt
     3961 aggcgcgcct atttctgaat tcccgatcta gtaacataga tgacaccgcg cgcgataatt
     4021 tatcctagtt tgcgcgctat attttgtttt ctatcgcgta ttaaatgtat aattgcggga
     4081 ctctaatcat aaaaacccat ctcataaata acgtcatgca ttacatgtta attattacat
     4141 gcttaacgta attcaacaga aattatatga taatcatcgc aagaccggca acaggattca
     4201 atcttaagaa actttattgc caaatgtttg aacgatcggg gatcatccgg gtctgtggcg
     4261 ggaactccac gaaaatatcc gaacgcagca agatatcgcg gtgcatctcg gtcttgcctg
     4321 ggcagtcgcc gccgacgccg ttgatgtgga cgccgggccc gatcatattg tcgctcagga
     4381 tcgtggcgtt gtgcttgtcg gccgttgctg tcgtaatgat atcggcacct tcgaccgcct
     4441 gttccgcaga gatcccgtgg gcgaagaact ccagcatgag atccccgcgc tggaggatca
     4501 tccagccggc gtcccggaaa acgattccga agcccaacct ttcatagaag gcggcggtgg
     4561 aatcgaaatc tcgtgatggc aggttgggcg tcgcttggtc ggtcatttcg aaccccagag
     4621 tcccgctcag aagaactcgt caagaaggcg atagaaggcg atgcgctgcg aatcgggagc
     4681 ggcgataccg taaagcacga ggaagcggtc agcccattcg ccgccaagct cttcagcaat
     4741 atcacgggta gccaacgcta tgtcctgata gcggtccgcc acacccagcc ggccacagtc
     4801 gatgaatcca gaaaagcggc cattttccac catgatattc ggcaagcagg catcgccatg
     4861 ggtcacgacg agatcatcgc cgtcgggcat gcgcgccttg agcctggcga acagttcggc
     4921 tggcgcgagc ccctgatgct cttcgtccag atcatcctga tcgacaagac cggcttccat
     4981 ccgagtacgt gctcgctcga tgcgatgttt cgcttggtgg tcgaatgggc aggtagccgg
     5041 atcaagcgta tgcagccgcc gcattgcatc agccatgatg gatactttct cggcaggagc
     5101 aaggtgagat gacaggagat cctgccccgg cacttcgccc aatagcagcc agtcccttcc
     5161 cgcttcagtg acaacgtcga gcacagctgc gcaaggaacg cccgtcgtgg ccagccacga
     5221 tagccgcgct gcctcgtcct gcagttcatt cagggcaccg gacaggtcgg tcttgacaaa
     5281 aagaaccggg cgcccctgcg ctgacagccg gaacacggcg gcatcagagc agccgattgt
     5341 ctgttgtgcc cagtcatagc cgaatagcct ctccacccaa gcggccggag aacctgcgtg
     5401 caatccatct tgttcaatca tgcgaaacga tccagatccg gtgcagatta tttggattga
     5461 gagtgaatat gagactctaa ttggataccg aggggaattt atggaacgtc agtggagcat
     5521 ttttgacaag aaatatttgc tagctgatag tgaccttagg cgacttttga acgcgcaata
     5581 atggtttctg acgtatgtgc ttagctcatt aaactccaga aacccgcggc tgagtggctc
     5641 cttcaacgtt gcggttctgt cagttccaaa cgtaaaacgg cttgtcccgc gtcatcggcg
     5701 ggggtcataa cgtgactccc ttaattctcc gctcatgatg aattcagaaa taaattcagc
     5761 ctaattcggc gttaattcag tacattaaaa acgtccgcaa tgtgttatta agttgtctaa
     5821 gcgtcaattt gtttacacca caatatatcc tgccaccagc cagccaacag ctccccgacc
     5881 ggcagctcgg cacaaaatca ccactcgata caggcagccc atcagtccgg gacggcgtca
     5941 gcgggagagc cgttgtaagg cggcagactt tgctcatgtt accgatgcta ttcggaagaa
     6001 cggcaactaa gctgccgggt ttgaaacacg gatgatctcg cggagggtag catgttgatt
     6061 gtaacgatga cagagcgttg ctgcctgtga tcaattcggg cacgaaccca gtggacataa
     6121 gcctgttcgg ttcgtaagct gtaatgcaag tagcgtatgc gctcacgcaa ctggtccaga
     6181 accttgaccg aacgcagcgg tggtaacggc gcagtggcgg ttttcatggc ttgttatgac
     6241 tgtttttttg gggtacagtc tatgcctcgg gcatccaagc agcaagcgcg ttacgccgtg
     6301 ggtcgatgtt tgatgttatg gagcagcaac gatgttacgc agcagggcag tcgccctaaa
     6361 acaaagttaa acatcatggg ggaagcggtg atcgccgaag tatcgactca actatcagag
     6421 gtagttggcg tcatcgagcg ccatctcgaa ccgacgttgc tggccgtaca tttgtacggc
     6481 tccgcagtgg atggcggcct gaagccacac agtgatattg atttgctggt tacggtgacc
     6541 gtaaggcttg atgaaacaac gcggcgagct ttgatcaacg accttttgga aacttcggct
     6601 tcccctggag agagcgagat tctccgcgct gtagaagtca ccattgttgt gcacgacgac
     6661 atcattccgt ggcgttatcc agctaagcgc gaactgcaat ttggagaatg gcagcgcaat
     6721 gacattcttg caggtatctt cgagccagcc acgatcgaca ttgatctggc tatcttgctg
     6781 acaaaagcaa gagaacatag cgttgccttg gtaggtccag cggcggagga actctttgat
     6841 ccggttcctg aacaggatct atttgaggcg ctaaatgaaa ccttaacgct atggaactcg
     6901 ccgcccgact gggctggcga tgagcgaaat gtagtgctta cgttgtcccg catttggtac
     6961 agcgcagtaa ccggcaaaat cgcgccgaag gatgtcgctg ccgactgggc aatggagcgc
     7021 ctgccggccc agtatcagcc cgtcatactt gaagctagac aggcttatct tggacaagaa
     7081 gaagatcgct tggcctcgcg cgcagatcag ttggaagaat ttgtccacta cgtgaaaggc
     7141 gagatcacca aggtagtcgg caaataatgt ctagctagaa attcgttcaa gccgacgccg
     7201 cttcgcggcg cggcttaact caagcgttag atgcactaag cacataattg ctcacagcca
     7261 aactatcagg tcaagtctgc ttttattatt tttaagcgtg cataataagc cctacacaaa
     7321 ttgggagata tatcatgcat gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg
     7381 tcagaccccg tagaaaagat caaaggatct tcttgagatc ctttttttct gcgcgtaatc
     7441 tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc ggatcaagag
     7501 ctaccaactc tttttccgaa ggtaactggc ttcagcagag cgcagatacc aaatactgtc
     7561 cttctagtgt agccgtagtt aggccaccac ttcaagaact ctgtagcacc gcctacatac
     7621 ctcgctctgc taatcctgtt accagtggct gctgccagtg gcgataagtc gtgtcttacc
     7681 gggttggact caagacgata gttaccggat aaggcgcagc ggtcgggctg aacggggggt
     7741 tcgtgcacac agcccagctt ggagcgaacg acctacaccg aactgagata cctacagcgt
     7801 gagctatgag aaagcgccac gcttcccgaa gggagaaagg cggacaggta tccggtaagc
     7861 ggcagggtcg gaacaggaga gcgcacgagg gagcttccag ggggaaacgc ctggtatctt
     7921 tatagtcctg tcgggtttcg ccacctctga cttgagcgtc gatttttgtg atgctcgtca
     7981 ggggggcgga gcctatggaa aaacgccagc aacgcggcct ttttacggtt cctggccttt
     8041 tgctggcctt ttgctcacat gttctttcct gcgttatccc ctgattctgt ggataaccgt
     8101 attaccgcct ttgagtgagc tgataccgct cgccgcagcc gaacgaccga gcgcagcgag
     8161 tcagtgagcg aggaagcgga agagcgcctg atgcggtatt ttctccttac gcatctgtgc
     8221 ggtatttcac accgcatatg gtgcactctc agtacaatct gctctgatgc cgcatagtta
     8281 agccagtata cactccgcta tcgctacgtg actgggtcat ggctgcgccc cgacacccgc
     8341 caacacccgc tgacgcgccc tgacgggctt gtctgctccc ggcatccgct tacagacaag
     8401 ctgtgaccgt ctccgggagc tgcatgtgtc agaggttttc accgtcatca ccgaaacgcg
     8461 cgaggcaggg tgccttgatg tgggcgccgg cggtcgagtg gcgacggcgc ggcttgtccg
     8521 cgccctggta gattgcctgg ccgtaggcca gccatttttg agcggccagc ggccgcgata
     8581 ggccgacgcg aagcggcggg gcgtagggag cgcagcgacc gaagggtagg cgctttttgc
     8641 agctcttcgg ctgtgcgctg gccagacagt tatgcacagg ccaggcgggt tttaagagtt
     8701 ttaataagtt ttaaagagtt ttaggcggaa aaatcgcctt ttttctcttt tatatcagtc
     8761 acttacatgt gtgaccggtt cccaatgtac ggctttgggt tcccaatgta cgggttccgg
     8821 ttcccaatgt acggctttgg gttcccaatg tacgtgctat ccacaggaaa gagacctttt
     8881 cgaccttttt cccctgctag ggcaatttgc cctagcatct gctccgtaca ttaggaaccg
     8941 gcggatgctt cgccctcgat caggttgcgg tagcgcatga ctaggatcgg gccagcctgc
     9001 cccgcctcct ccttcaaatc gtactccggc aggtcatttg acccgatcag cttgcgcacg
     9061 gtgaaacaga acttcttgaa ctctccggcg ctgccactgc gttcgtagat cgtcttgaac
     9121 aaccatctgg cttctgcctt gcctgcggcg cggcgtgcca ggcggtagag aaaacggccg
     9181 atgccgggat cgatcaaaaa gtaatcgggg tgaaccgtca gcacgtccgg gttcttgcct
     9241 tctgtgatct cgcggtacat ccaatcagct agctcgatct cgatgtactc cggccgcccg
     9301 gtttcgctct ttacgatctt gtagcggcta atcaaggctt caccctcgga taccgtcacc
     9361 aggcggccgt tcttggcctt cttcgtacgc tgcatggcaa cgtgcgtggt gtttaaccga
     9421 atgcaggttt ctaccaggtc gtctttctgc tttccgccat cggctcgccg gcagaacttg
     9481 agtacgtccg caacgtgtgg acggaacacg cggccgggct tgtctccctt cccttcccgg
     9541 tatcggttca tggattcggt tagatgggaa accgccatca gtaccaggtc gtaatcccac
     9601 acactggcca tgccggccgg ccctgcggaa acctctacgt gcccgtctgg aagctcgtag
     9661 cggatcacct cgccagctcg tcggtcacgc ttcgacagac ggaaaacggc cacgtccatg
     9721 atgctgcgac tatcgcgggt gcccacgtca tagagcatcg gaacgaaaaa atctggttgc
     9781 tcgtcgccct tgggcggctt cctaatcgac ggcgcaccgg ctgccggcgg ttgccgggat
     9841 tctttgcgga ttcgatcagc ggccgcttgc cacgattcac cggggcgtgc ttctgcctcg
     9901 atgcgttgcc gctgggcggc ctgcgcggcc ttcaacttct ccaccaggtc atcacccagc
     9961 gccgcgccga tttgtaccgg gccggatggt ttgcgaccgc tcacgccgat tcctcgggct
    10021 tgggggttcc agtgccattg cagggccggc agacaaccca gccgcttacg cctggccaac
    10081 cgcccgttcc tccacacatg gggcattcca cggcgtcggt gcctggttgt tcttgatttt
    10141 ccatgccgcc tcctttagcc gctaaaattc atctactcat ttattcattt gctcatttac
    10201 tctggtagct gcgcgatgta ttcagatagc agctcggtaa tggtcttgcc ttggcgtacc
    10261 gcgtacatct tcagcttggt gtgatcctcc gccggcaact gaaagttgac ccgcttcatg
    10321 gctggcgtgt ctgccaggct ggccaacgtt gcagccttgc tgctgcgtgc gctcggacgg
    10381 ccggcactta gcgtgtttgt gcttttgctc attttctctt tacctcatta actcaaatga
    10441 gttttgattt aatttcagcg gccagcgcct ggacctcgcg ggcagcgtcg ccctcgggtt
    10501 ctgattcaag aacggttgtg ccggcggcgg cagtgcctgg gtagctcacg cgctgcgtga
    10561 tacgggactc aagaatgggc agctcgtacc cggccagcgc ctcggcaacc tcaccgccga
    10621 tgcgcgtgcc tttgatcgcc cgcgacacga caaaggccgc ttgtagcctt ccatccgtga
    10681 cctcaatgcg ctgcttaacc agctccacca ggtcggcggt ggcccatatg tcgtaagggc
    10741 ttggctgcac cggaatcagc acgaagtcgg ctgccttgat cgcggacaca gccaagtccg
    10801 ccgcctgggg cgctccgtcg atcactacga agtcgcgccg gccgatggcc ttcacgtcgc
    10861 ggtcaatcgt cgggcggtcg atgccgacaa cggttagcgg ttgatcttcc cgcacggccg
    10921 cccaatcgcg ggcactgccc tggggatcgg aatcgactaa cagaacatcg gccccggcga
    10981 gttgcagggc gcgggctaga tgggttgcga tggtcgtctt gcctgacccg cctttctggt
    11041 taagtacagc gataaccttc atgcgttccc cttgcgtatt tgtttattta ctcatcgcat
    11101 catatacgca gcgaccgcat gacgcaagct gttttactca aatacacatc acctttttag
    11161 acggcggcgc tcggtttctt cagcggccaa gctggccggc caggccgcca gcttggcatc
    11221 agacaaaccg gccaggattt catgcagccg cacggttgag acgtgcgcgg gcggctcgaa
    11281 cacgtacccg gccgcgatca tctccgcctc gatctcttcg gtaatgaaaa acggttcgtc
    11341 ctggccgtcc tggtgcggtt tcatgcttgt tcctcttggc gttcattctc ggcggccgcc
    11401 agggcgtcgg cctcggtcaa tgcgtcctca cggaaggcac cgcgccgcct ggcctcggtg
    11461 ggcgtcactt cctcgctgcg ctcaagtgcg cggtacaggg tcgagcgatg cacgccaagc
    11521 agtgcagccg cctctttcac ggtgcggcct tcctggtcga tcagctcgcg ggcgtgcgcg
    11581 atctgtgccg gggtgagggt agggcggggg ccaaacttca cgcctcgggc cttggcggcc
    11641 tcgcgcccgc tccgggtgcg gtcgatgatt agggaacgct cgaactcggc aatgccggcg
    11701 aacacggtca acaccatgcg gccggccggc gtggtggtgt cggcccacgg ctctgccagg
    11761 ctacgcaggc ccgcgccggc ctcctggatg cgctcggcaa tgtccagtag gtcgcgggtg
    11821 ctgcgggcca ggcggtctag cctggtcact gtcacaacgt cgccagggcg taggtggtca
    11881 agcatcctgg ccagctccgg gcggtcgcgc ctggtgccgg tgatcttctc ggaaaacagc
    11941 ttggtgcagc cggccgcgtg cagttcggcc cgttggttgg tcaagtcctg gtcgtcggtg
    12001 ctgacgcggg catagcccag caggccagcg gcggcgctct tgttcatggc gtaatgtctc
    12061 cggttctagt cgcaagtatt ctactttatg cgactaaaac acgcgacaag aaaacgccag
    12121 gaaaagggca gggcggcagc ctgtcgcgta acttaggact tgtgcgacat gtcgttttca
    12181 gaagacggct gcactgaacg tcagaagccg actgcactat agcagcggag gggttggatc
    12241 aaagtacttt gatcccgagg ggaaccctgt ggttggcatg cacatacaaa tggacgaacg
    12301 gataaacct
//
