LOCUS       sequence-496476-       12477 bp    DNA     circular SYN 07-JAN-2026
DEFINITION  Stable transformation or transient expression of gene of interest in
            plants.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12477)
  AUTHORS   Wan WL, Kim G, Kim N, Tan YY, Watari M, Charoennit N, Lee RRQ, Phang
            IRK, Wang J, Liew YY, Hu D, Ng SK, Zhang Y, Jang IC, Song JJ, Chae 
            E.
  TITLE     Structural determinants of DANGEROUS MIX 3, an alpha/beta hydrolase 
            that triggers NLR-mediated genetic incompatibility in plants.
  JOURNAL   Molecular Cell, Volume 85, Issue 14
REFERENCE   2  (bases 1 to 12477)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Jan 7, 2026 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Molecular 
            Cell, Volume 85, Issue 14"
COMMENT     Sequence Label: pGWB512_35S-FLAG-DM3(Hh-0)68-510
FEATURES             Location/Qualifiers
     source          1..12477
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    54..78
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     primer_bind     266..288
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     280..297
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     281..297
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        812..1157
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     primer_bind     1100..1120
                     /label=35S promoter
                     /note="CaMV 35S promoter, forward primer"
     protein_bind    1179..1203
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(3319..3343)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             3354..3377
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     terminator      3399..3651
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     primer_bind     complement(3684..3700)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3684..3700)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(3697..3719)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    3708..3724
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(3732..3738,3739..3756,3757..3762))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    3777..3798
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     terminator      complement(3834..4086)
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     CDS             complement(4237..5262)
                     /codon_start=1
                     /gene="aph(4)-Ia"
                     /product="aminoglycoside phosphotransferase from E. coli"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGAFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     promoter        complement(5314..5493)
                     /label=NOS promoter
                     /note="nopaline synthase promoter"
     misc_feature    5999..6023
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     CDS             6544..7335
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MGEAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      7581..8169
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     8070..8089
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     8323..8340
                     /label=L4440
                     /note="L4440 vector, forward primer"
     misc_feature    8355..8495
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     complement(8421..8440)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     8581..8603
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     rep_origin      8839..9033
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     CDS             complement(9099..10172)
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="MSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
                     QAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
                     KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
                     GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
                     ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
                     LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
                     LVMRYRNLIEGEASAGS"
     CDS             complement(10601..11230)
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
ORIGIN
        1 tttcacgccc ttttaaatat ccgattattc taataaacgc tcttttctct taggtttacc
       61 cgccaatata tcctgtcaaa cactgatagt ttaaactgaa ggcgggaaac gacaatctga
      121 tccaagctca agctgctcta gcattcgcca ttcaggctgc gcaactgttg ggaagggcga
      181 tcggtgcggg cctcttcgct attacgccag ctggcgaaag ggggatgtgc tgcaaggcga
      241 ttaagttggg taacgccagg gttttcccag tcacgacgtt gtaaaacgac ggccagtgcc
      301 aagcttgcat gcctgcaggt ccccagatta gccttttcaa tttcagaaag aatgctaacc
      361 cacagatggt tagagaggct tacgcagcag gtctcatcaa gacgatctac ccgagcaata
      421 atctccagga aatcaaatac cttcccaaga aggttaaaga tgcagtcaaa agattcagga
      481 ctaactgcat caagaacaca gagaaagata tatttctcaa gatcagaagt actattccag
      541 tatggacgat tcaaggcttg cttcacaaac caaggcaagt aatagagatt ggagtctcta
      601 aaaaggtagt tcccactgaa tcaaaggcca tggagtcaaa gattcaaata gaggacctaa
      661 cagaactcgc cgtaaagact ggcgaacagt tcatacagag tctcttacga ctcaatgaca
      721 agaagaaaat cttcgtcaac atggtggagc acgacacact tgtctactcc aaaaatatca
      781 aagatacagt ctcagaagac caaagggcaa ttgagacttt tcaacaaagg gtaatatccg
      841 gaaacctcct cggattccat tgcccagcta tctgtcactt tattgtgaag atagtggaaa
      901 aggaaggtgg ctcctacaaa tgccatcatt gcgataaagg aaaggccatc gttgaagatg
      961 cctctgccga cagtggtccc aaagatggac ccccacccac gaggagcatc gtggaaaaag
     1021 aagacgttcc aaccacgtct tcaaagcaag tggattgatg tgatatctcc actgacgtaa
     1081 gggatgacgc acaatcccac tatccttcgc aagacccttc ctctatataa ggaagttcat
     1141 ttcatttgga gagaacacgg gggactctag agttatcaac aagtttgtac aaaaaagcag
     1201 gctttcgaat tccaatggag catgttactg ggaagtggtt ttcggtgccg gagctccgac
     1261 tgcgtgacca ccggttcata gttcctcttg attattccaa aagttctccc aaaattaccg
     1321 tctttgctcg agagatcgtt gcgggtaact tgaaatttgg agctttttga ttcagagctc
     1381 tttctgtgat tacgaagcta aaagtttgtg tcttttgttt tttgtagttg ggaaagagga
     1441 gcaagcaatg ccgtatttat tgtatttgca aggtggtcca ggatttgaag gtccaaggcc
     1501 tagtgaagct agtggatgga ttcaaagagc ttgtgaagag tttcgagtgg tcttactcga
     1561 tcaagtatga tgctttcata gttttatact cttctctgga cagttttttt agtctttttg
     1621 ttgttctaat tttgtttgtc ctgcagcgag gaacaggctt gtccactcct ttaatatgtt
     1681 cctctatgct tcaattcaaa tcagcaaagg aattagctga ttatttggtt cactttcgag
     1741 ctgataatat agttaaagat gctgagttta ttagagttcg tcttgttcct aaggctgatc
     1801 cttggacaat tttgggtcag gtaatttgtc tttgtattgt ctattggtca tcttgcttca
     1861 gttcttgtca tttgattttg tattcatctt tttttcttca aatctacaga gtttcggtgg
     1921 cttttgtgca cttacatatc tgagttttgc acccgaagga ctcaaacaag tgctaataac
     1981 tggaggaatt ccaccaattg gtaaagcatg tacagcagat gatgtatatg aagccggttt
     2041 tgaacaagtt gctcgccaaa atgaaaaata ctacaagaga ttccctcaag acattgagat
     2101 tgttcgtgaa ctcgtaaatt acttggctga atctgaaggt ggcggggtga gggattacaa
     2161 catttgctca atccactgac cagtttagtt tagttcctta cttgtctctg tttctttgtc
     2221 tttaggtacc tctcccatca ggaggcattc ttactcctaa gggtcttcaa actcttggtc
     2281 tttctggttt aggatcaagt actggttttg agcgcctgca ttatatgtaa gagtttagct
     2341 taatttttat caaccacctc acatgttctg tgttaaatct ggccttctta attgatcata
     2401 ggttggagag agtatgggat cctattttag ttactggagc tccaaagtgt attagtcagt
     2461 tcttcttaaa cgctgtaaga gacccacaaa aatttgttgt tttctgttct cagctcaaat
     2521 aaggaattca atagtctgag acattttatc gttgctgcag tttgagagtt ggcattcttt
     2581 tgatgcaaat ccactatatg ctcttctcca cgaggccata tactgtgagg tatagtctct
     2641 tcaaatttct catctttttt tctatttcag acaattcctt cttcttgtta tcatttatgt
     2701 ttaaatggaa actttttcag ggtgcttcat caggatggtc tgctcataga ttacgggata
     2761 aatatgagta caaattcgac gcaatgaagg cagtcaaaga aagtcagcct gtactcttca
     2821 caggagaggt acaggttttg agtatttcta attgtatggc tcatagcttg gatgattggt
     2881 ccacattgct taactaaaat agaatttgga tttgtataga tgatatttcc atggatgttt
     2941 gatgaaattc atgccttgaa gccatttaaa gctgccgccg atcttttggc caagaaagag
     3001 gattggcctc cattatatga tgttcctaga ttgcagaata acaaggtctg tttcagaatc
     3061 ttgatcgttc tttcatatgg atcaaataat tttactctgt ttcacatgat ttctttttgt
     3121 aggtgcctgt tgctgcagcg gtttactacg aagatatgta tgtaaacttc aagctggtga
     3181 cggaaacagc ttctcatata tcgggtatca gactttgggt aacgaatgag tttatgcatt
     3241 cgggtcttcg tgatgctgga agacaaatta ttgatcattt gttgggaatg atcaatgggt
     3301 gaccgaattc ctatctagac ccagctttct tgtacaaagt ggttgataac agcgactaca
     3361 aggatgacga tgacaaggct tagagctcga atttccccga tcgttcaaac atttggcaat
     3421 aaagtttctt aagattgaat cctgttgccg gtcttgcgat gattatcata taatttctgt
     3481 tgaattacgt taagcatgta ataattaaca tgtaatgcat gacgttattt atgagatggg
     3541 tttttatgat tagagtcccg caattataca tttaatacgc gatagaaaac aaaatatagc
     3601 gcgcaaacta ggataaatta tcgcgcgcgg tgtcatctat gttactagat cgggaattgg
     3661 ttccggaacc aattcgtaat catgtcatag ctgtttcctg tgtgaaattg ttatccgctc
     3721 acaattccac acaacatacg agccggaagc ataaagtgta aagcctgggg tgcctaatga
     3781 gtgagctaac tcacattagg ctgaattagg cgcgcctatt tctattaatt cccgatctag
     3841 taacatagat gacaccgcgc gcgataattt atcctagttt gcgcgctata ttttgttttc
     3901 tatcgcgtat taaatgtata attgcgggac tctaatcata aaaacccatc tcataaataa
     3961 cgtcatgcat tacatgttaa ttattacatg cttaacgtaa ttcaacagaa attatatgat
     4021 aatcatcgca agaccggcaa caggattcaa tcttaagaaa ctttattgcc aaatgtttga
     4081 acgatcgggg aaattcgggg gatcgatccc gcgaactgtg gacgagaact gtgccaccaa
     4141 gcgtaaggcc gttctctcgc atttgccttg ctaggctcgc gcgagttgct ggctgaggcg
     4201 ttctcgaaat cagctcttgt tcggtcggca tctactctat tcctttgccc tcggacgagt
     4261 gctggggcgt cggtttccac tatcggcgag tacttctaca cagccatcgg tccagacggc
     4321 cgcgcttctg cgggcgattt gtgtacgccc gacagtcccg gctccggatc ggacgattgc
     4381 gtcgcatcga ccctgcgccc aagctgcatc atcgaaattg ccgtcaacca agctctgata
     4441 gagttggtca agaccaatgc ggagcatata cgcccggagc cgcggcgatc ctgcaagctc
     4501 cggatgcctc cgctcgaagt agcgcgtctg ctgctccata caagccaacc acggcctcca
     4561 gaagaagatg ttggcgacct cgtattggga atccccgaac atcgcctcgc tccagtcaat
     4621 gaccgctgtt atgcggccat tgtccgtcag gacattgttg gagccgaaat ccgcgtgcac
     4681 gaggtgccgg acttcggggc agtcctcggc ccaaagcatc agctcatcga gagcctgcgc
     4741 gacggacgca ctgacggtgt cgtccatcac agtttgccag tgatacacat ggggatcagc
     4801 aatcgcgcat atgaaatcac gccatgtagt gtattgaccg attccttgcg gtccgaatgg
     4861 gccgaacccg ctcgtctggc taagatcggc cgcagcgatc gcatccatgg cctccgcgac
     4921 cggctgcaga acagcgggca gttcggtttc aggcaggtct tgcaacgtga caccctgtgc
     4981 acggcgggag atgcaatagg tcaggctctc gctgaatgcc ccaatgtcaa gcacttccgg
     5041 aatcgggagc gcggccgatg caaagtgccg ataaacataa cgatctttgt agaaaccatc
     5101 ggcgcagcta tttacccgca ggacatatcc acgccctcct acatcgaagc tgaaagcacg
     5161 agattcttcg ccctccgaga gctgcatcag gtcggagacg ctgtcgaact tttcgatcag
     5221 aaacttctcg acagacgtcg cggtgagttc aggctttttc atatcttatt gccccccggg
     5281 atcagatctg gattgagagt gaatatgaga ctctaattgg ataccgaggg gaatttatgg
     5341 aacgtcagtg gagcattttt gacaagaaat atttgctagc tgatagtgac cttaggcgac
     5401 ttttgaacgc gcaataatgg tttctgacgt atgtgcttag ctcattaaac tccagaaacc
     5461 cgcggctgag tggctccttc aacgttgcgg ttctgtcagt tccaaacgta aaacggcttg
     5521 tcccgcgtca tcggcggggg tcataacgtg actcccttaa ttctccgctc atgatcttga
     5581 tcccctgcgc catcagatcc ttggcggcaa gaaagccatc cagtttactt tgcagggctt
     5641 cccaacctta ccagagggcg ccccagctgg caattccggt tcgcttgctg tccataaaac
     5701 cgcccagtct agctatcgcc atgtaagccc actgcaagct acctgctttc tctttgcgct
     5761 tgcgttttcc cttgtccaga tagcccagta gctgacattc atccggggtc agcaccgttt
     5821 ctgcggactg gctttctacg tgttccgctt cctttagcag cccttgcgcc ctgagtgctt
     5881 gcggcagcgt gaagctgatc cgtcgagatt aatagaaata aattcagcct aattcggcgt
     5941 taattcagta cattaaaaac gtccgcaatg tgttattaag ttgtctaagc gtcaatttgt
     6001 ttacaccaca atatatcctg ccaccagcca gccaacagct ccccgaccgg cagctcggca
     6061 caaaatcacc actcgataca ggcagcccat cagtccggga cggcgtcagc gggagagccg
     6121 ttgtaaggcg gcagactttg ctcatgttac cgatgctatt cggaagaacg gcaactaagc
     6181 tgccgggttt gaaacacgga tgatctcgcg gagggtagca tgttgattgt aacgatgaca
     6241 gagcgttgct gcctgtgatc aattcgggca cgaacccagt ggacataagc ctgttcggtt
     6301 cgtaagctgt aatgcaagta gcgtatgcgc tcacgcaact ggtccagaac cttgaccgaa
     6361 cgcagcggtg gtaacggcgc agtggcggtt ttcatggctt gttatgactg tttttttggg
     6421 gtacagtcta tgcctcgggc atccaagcag caagcgcgtt acgccgtggg tcgatgtttg
     6481 atgttatgga gcagcaacga tgttacgcag cagggcagtc gccctaaaac aaagttaaac
     6541 atcatggggg aagcggtgat cgccgaagta tcgactcaac tatcagaggt agttggcgtc
     6601 atcgagcgcc atctcgaacc gacgttgctg gccgtacatt tgtacggctc cgcagtggat
     6661 ggcggcctga agccacacag tgatattgat ttgctggtta cggtgaccgt aaggcttgat
     6721 gaaacaacgc ggcgagcttt gatcaacgac cttttggaaa cttcggcttc ccctggagag
     6781 agcgagattc tccgcgctgt agaagtcacc attgttgtgc acgacgacat cattccgtgg
     6841 cgttatccag ctaagcgcga actgcaattt ggagaatggc agcgcaatga cattcttgca
     6901 ggtatcttcg agccagccac gatcgacatt gatctggcta tcttgctgac aaaagcaaga
     6961 gaacatagcg ttgccttggt aggtccagcg gcggaggaac tctttgatcc ggttcctgaa
     7021 caggatctat ttgaggcgct aaatgaaacc ttaacgctat ggaactcgcc gcccgactgg
     7081 gctggcgatg agcgaaatgt agtgcttacg ttgtcccgca tttggtacag cgcagtaacc
     7141 ggcaaaatcg cgccgaagga tgtcgctgcc gactgggcaa tggagcgcct gccggcccag
     7201 tatcagcccg tcatacttga agctagacag gcttatcttg gacaagaaga agatcgcttg
     7261 gcctcgcgcg cagatcagtt ggaagaattt gtccactacg tgaaaggcga gatcaccaag
     7321 gtagtcggca aataatgtct agctagaaat tcgttcaagc cgacgccgct tcgcggcgcg
     7381 gcttaactca agcgttagat gcactaagca cataattgct cacagccaaa ctatcaggtc
     7441 aagtctgctt ttattatttt taagcgtgca taataagccc tacacaaatt gggagatata
     7501 tcatgcatga ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc agaccccgta
     7561 gaaaagatca aaggatcttc ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa
     7621 acaaaaaaac caccgctacc agcggtggtt tgtttgccgg atcaagagct accaactctt
     7681 tttccgaagg taactggctt cagcagagcg cagataccaa atactgtcct tctagtgtag
     7741 ccgtagttag gccaccactt caagaactct gtagcaccgc ctacatacct cgctctgcta
     7801 atcctgttac cagtggctgc tgccagtggc gataagtcgt gtcttaccgg gttggactca
     7861 agacgatagt taccggataa ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag
     7921 cccagcttgg agcgaacgac ctacaccgaa ctgagatacc tacagcgtga gctatgagaa
     7981 agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga
     8041 acaggagagc gcacgaggga gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc
     8101 gggtttcgcc acctctgact tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc
     8161 ctatggaaaa acgccagcaa cgcggccttt ttacggttcc tggccttttg ctggcctttt
     8221 gctcacatgt tctttcctgc gttatcccct gattctgtgg ataaccgtat taccgccttt
     8281 gagtgagctg ataccgctcg ccgcagccga acgaccgagc gcagcgagtc agtgagcgag
     8341 gaagcggaag agcgcctgat gcggtatttt ctccttacgc atctgtgcgg tatttcacac
     8401 cgcatatggt gcactctcag tacaatctgc tctgatgccg catagttaag ccagtataca
     8461 ctccgctatc gctacgtgac tgggtcatgg ctgcgccccg acacccgcca acacccgctg
     8521 acgcgccctg acgggcttgt ctgctcccgg catccgctta cagacaagct gtgaccgtct
     8581 ccgggagctg catgtgtcag aggttttcac cgtcatcacc gaaacgcgcg aggcagggtg
     8641 ccttgatgtg ggcgccggcg gtcgagtggc gacggcgcgg cttgtccgcg ccctggtaga
     8701 ttgcctggcc gtaggccagc catttttgag cggccagcgg ccgcgatagg ccgacgcgaa
     8761 gcggcggggc gtagggagcg cagcgaccga agggtaggcg ctttttgcag ctcttcggct
     8821 gtgcgctggc cagacagtta tgcacaggcc aggcgggttt taagagtttt aataagtttt
     8881 aaagagtttt aggcggaaaa atcgcctttt ttctctttta tatcagtcac ttacatgtgt
     8941 gaccggttcc caatgtacgg ctttgggttc ccaatgtacg ggttccggtt cccaatgtac
     9001 ggctttgggt tcccaatgta cgtgctatcc acaggaaaga gaccttttcg acctttttcc
     9061 cctgctaggg caatttgccc tagcatctgc tccgtacatt aggaaccggc ggatgcttcg
     9121 ccctcgatca ggttgcggta gcgcatgact aggatcgggc cagcctgccc cgcctcctcc
     9181 ttcaaatcgt actccggcag gtcatttgac ccgatcagct tgcgcacggt gaaacagaac
     9241 ttcttgaact ctccggcgct gccactgcgt tcgtagatcg tcttgaacaa ccatctggct
     9301 tctgccttgc ctgcggcgcg gcgtgccagg cggtagagaa aacggccgat gccgggatcg
     9361 atcaaaaagt aatcggggtg aaccgtcagc acgtccgggt tcttgccttc tgtgatctcg
     9421 cggtacatcc aatcagctag ctcgatctcg atgtactccg gccgcccggt ttcgctcttt
     9481 acgatcttgt agcggctaat caaggcttca ccctcggata ccgtcaccag gcggccgttc
     9541 ttggccttct tcgtacgctg catggcaacg tgcgtggtgt ttaaccgaat gcaggtttct
     9601 accaggtcgt ctttctgctt tccgccatcg gctcgccggc agaacttgag tacgtccgca
     9661 acgtgtggac ggaacacgcg gccgggcttg tctcccttcc cttcccggta tcggttcatg
     9721 gattcggtta gatgggaaac cgccatcagt accaggtcgt aatcccacac actggccatg
     9781 ccggccggcc ctgcggaaac ctctacgtgc ccgtctggaa gctcgtagcg gatcacctcg
     9841 ccagctcgtc ggtcacgctt cgacagacgg aaaacggcca cgtccatgat gctgcgacta
     9901 tcgcgggtgc ccacgtcata gagcatcgga acgaaaaaat ctggttgctc gtcgcccttg
     9961 ggcggcttcc taatcgacgg cgcaccggct gccggcggtt gccgggattc tttgcggatt
    10021 cgatcagcgg ccgcttgcca cgattcaccg gggcgtgctt ctgcctcgat gcgttgccgc
    10081 tgggcggcct gcgcggcctt caacttctcc accaggtcat cacccagcgc cgcgccgatt
    10141 tgtaccgggc cggatggttt gcgaccgctc acgccgattc ctcgggcttg ggggttccag
    10201 tgccattgca gggccggcag acaacccagc cgcttacgcc tggccaaccg cccgttcctc
    10261 cacacatggg gcattccacg gcgtcggtgc ctggttgttc ttgattttcc atgccgcctc
    10321 ctttagccgc taaaattcat ctactcattt attcatttgc tcatttactc tggtagctgc
    10381 gcgatgtatt cagatagcag ctcggtaatg gtcttgcctt ggcgtaccgc gtacatcttc
    10441 agcttggtgt gatcctccgc cggcaactga aagttgaccc gcttcatggc tggcgtgtct
    10501 gccaggctgg ccaacgttgc agccttgctg ctgcgtgcgc tcggacggcc ggcacttagc
    10561 gtgtttgtgc ttttgctcat tttctcttta cctcattaac tcaaatgagt tttgatttaa
    10621 tttcagcggc cagcgcctgg acctcgcggg cagcgtcgcc ctcgggttct gattcaagaa
    10681 cggttgtgcc ggcggcggca gtgcctgggt agctcacgcg ctgcgtgata cgggactcaa
    10741 gaatgggcag ctcgtacccg gccagcgcct cggcaacctc accgccgatg cgcgtgcctt
    10801 tgatcgcccg cgacacgaca aaggccgctt gtagccttcc atccgtgacc tcaatgcgct
    10861 gcttaaccag ctccaccagg tcggcggtgg cccatatgtc gtaagggctt ggctgcaccg
    10921 gaatcagcac gaagtcggct gccttgatcg cggacacagc caagtccgcc gcctggggcg
    10981 ctccgtcgat cactacgaag tcgcgccggc cgatggcctt cacgtcgcgg tcaatcgtcg
    11041 ggcggtcgat gccgacaacg gttagcggtt gatcttcccg cacggccgcc caatcgcggg
    11101 cactgccctg gggatcggaa tcgactaaca gaacatcggc cccggcgagt tgcagggcgc
    11161 gggctagatg ggttgcgatg gtcgtcttgc ctgacccgcc tttctggtta agtacagcga
    11221 taaccttcat gcgttcccct tgcgtatttg tttatttact catcgcatca tatacgcagc
    11281 gaccgcatga cgcaagctgt tttactcaaa tacacatcac ctttttagac ggcggcgctc
    11341 ggtttcttca gcggccaagc tggccggcca ggccgccagc ttggcatcag acaaaccggc
    11401 caggatttca tgcagccgca cggttgagac gtgcgcgggc ggctcgaaca cgtacccggc
    11461 cgcgatcatc tccgcctcga tctcttcggt aatgaaaaac ggttcgtcct ggccgtcctg
    11521 gtgcggtttc atgcttgttc ctcttggcgt tcattctcgg cggccgccag ggcgtcggcc
    11581 tcggtcaatg cgtcctcacg gaaggcaccg cgccgcctgg cctcggtggg cgtcacttcc
    11641 tcgctgcgct caagtgcgcg gtacagggtc gagcgatgca cgccaagcag tgcagccgcc
    11701 tctttcacgg tgcggccttc ctggtcgatc agctcgcggg cgtgcgcgat ctgtgccggg
    11761 gtgagggtag ggcgggggcc aaacttcacg cctcgggcct tggcggcctc gcgcccgctc
    11821 cgggtgcggt cgatgattag ggaacgctcg aactcggcaa tgccggcgaa cacggtcaac
    11881 accatgcggc cggccggcgt ggtggtgtcg gcccacggct ctgccaggct acgcaggccc
    11941 gcgccggcct cctggatgcg ctcggcaatg tccagtaggt cgcgggtgct gcgggccagg
    12001 cggtctagcc tggtcactgt cacaacgtcg ccagggcgta ggtggtcaag catcctggcc
    12061 agctccgggc ggtcgcgcct ggtgccggtg atcttctcgg aaaacagctt ggtgcagccg
    12121 gccgcgtgca gttcggcccg ttggttggtc aagtcctggt cgtcggtgct gacgcgggca
    12181 tagcccagca ggccagcggc ggcgctcttg ttcatggcgt aatgtctccg gttctagtcg
    12241 caagtattct actttatgcg actaaaacac gcgacaagaa aacgccagga aaagggcagg
    12301 gcggcagcct gtcgcgtaac ttaggacttg tgcgacatgt cgttttcaga agacggctgc
    12361 actgaacgtc agaagccgac tgcactatag cagcggaggg gttggatcaa agtactttga
    12421 tcccgagggg aaccctgtgg ttggcatgca catacaaatg gacgaacgga taaacct
//
