LOCUS       sequence-496538-       13151 bp    DNA     circular SYN 07-JAN-2026
DEFINITION  Overexpression vector for FLAG-tagged UBR5..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 13151)
  AUTHORS   Tsai JM, Aguirre JD, Li YD, Brown J, Focht V, Kater L, Kempf G, 
            Sandoval B, Schmitt S, Rutter JC, Galli P, Sandate CR, Cutler JA, 
            Zou C, Donovan KA, Lumpkin RJ, Cavadini S, Park PMC, Sievers Q, 
            Hatton C, Ener E, Regalado BD, Sperling MT, Slabicki M, Kim J, Zon 
            R, Zhang Z, Miller PG, Belizaire R, Sperling AS, Fischer ES, 
            Irizarry R, Armstrong SA, Thoma NH, Ebert BL
  TITLE     UBR5 forms ligand-dependent complexes on chromatin to regulate 
            nuclear hormone receptor stability.
  JOURNAL   Mol Cell. 2023 Aug 3;83(15):2753-2767.e10. doi: 
            10.1016/j.molcel.2023.06.028. Epub 2023 Jul 20.
  PUBMED    37478846
REFERENCE   2  (bases 1 to 13151)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Jan 7, 2026 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.molcel.2023.06.028"; journalName: "Mol Cell"; date: 
            "2023-08-3- 3"; volume: "83"; issue: "15"; pages: "2753-2767.e10"
COMMENT     Sequence Label: pMH180_pDEST_CMV_UBR5_Flag
FEATURES             Location/Qualifiers
     source          1..13151
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     126..148
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     140..157
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     141..157
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     enhancer        318..697
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        698..901
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     852..872
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     CDS             935..958
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     protein_bind    995..1019
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(9434..9458)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     polyA_signal    9492..10114
                     /label=hGH poly(A) signal
                     /note="human growth hormone polyadenylation signal"
     primer_bind     complement(9691..9710)
                     /label=hGH-PA-R
                     /note="Human growth hormone terminator, reverse primer"
     primer_bind     complement(10138..10158)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        10143..10472
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      10323..10458
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(10510..10529)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(10511..10529)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(10543..10559)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(10543..10559)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(10556..10578)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    10567..10583
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(10591..10597,10598..10615,10616..10621))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    10636..10657
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(10774..10791)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(10945..11533)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(11025..11044)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(11704..12495,12496..12564))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     12327..12346
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(12565..12669)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      12696..13124
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(12783..12802)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     12993..13014
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
ORIGIN
        1 ccattcgcca ttcaggctgc gcaactgttg ggaagggcga tcggtgcggg cctcttcgct
       61 attacgccag ctggcgaaag ggggatgtgc tgcaaggcga ttaagttggg taacgccagg
      121 gttttcccag tcacgacgtt gtaaaacgac ggccagtgcc aagctgatct atacattgaa
      181 tcaatattgg caattagcca tattagtcat tggttatata gcataaatca atattggcta
      241 ttggccattg catacgttgt atctatatca taatatgtac atttatattg gctcatgtcc
      301 aatatgaccg ccatgttgac attgattatt gactagttat taatagtaat caattacggg
      361 gtcattagtt catagcccat atatggagtt ccgcgttaca taacttacgg taaatggccc
      421 gcctggctga ccgcccaacg acccccgccc attgacgtca ataatgacgt atgttcccat
      481 agtaacgcca atagggactt tccattgacg tcaatgggtg gagtatttac ggtaaactgc
      541 ccacttggca gtacatcaag tgtatcatat gccaagtccg ccccctattg acgtcaatga
      601 cggtaaatgg cccgcctggc attatgccca gtacatgacc ttacgggact ttcctacttg
      661 gcagtacatc tacgtattag tcatcgctat taccatggtg atgcggtttt ggcagtacac
      721 caatgggcgt ggatagcggt ttgactcacg gggatttcca agtctccacc ccattgacgt
      781 caatgggagt ttgttttggc accaaaatca acgggacttt ccaaaatgtc gtaataaccc
      841 cgccccgttg acgcaaatgg gcggtaggcg tgtacggtgg gaggtctata taagcagagc
      901 tcgtttagtg aaccgtcaga attgatctac catggactac aaagacgatg acgacaagct
      961 tgcggccgcg aattcatcga tagatctgat atcaacaagt ttgtacaaaa aagcaggctt
     1021 ccggaccatg acgtccatcc atttcgtggt tcacccgctg ccgggcaccg aggaccagct
     1081 caatgacagg ttacgagaag tttctgagaa gctgaacaaa tataatttaa acagccaccc
     1141 ccctttgaat gtattggaac aggctactat taaacagtgt gtggtgggac caaatcatgc
     1201 tgcctttctt cttgaggatg gtagagtttg caggattggt ttttcagtac agccagacag
     1261 attggaattg ggtaaacctg ataataatga tgggtcaaag ttgaacagca actcgggggc
     1321 agggaggacg tcaaggcctg gtaggacaag cgactctcca tggtttctct caggttctga
     1381 gactctaggc aggctggcag gcaacacctt aggaagccgc tggagttctg gagtgggtgg
     1441 aagtggtgga ggatcctctg gtaggtcatc agctggagct cgagattccc gccggcagac
     1501 tcgagttatt cggacaggac gggatcgagg gtctgggctt ttgggcagtc agccccagcc
     1561 agttattcca gcatctgtca ttccagagga gctgatttca caggcccaag ttgttttaca
     1621 aggcaaatcc agaagtgtca ttattcgaga acttcagaga acaaatcttg atgtgaacct
     1681 tgctgtaaat aatttactta gccgggatga tgaagatgga gatgatgggg atgatacagc
     1741 cagcgaatct tatttgcctg gagaggatct tatgtctctc cttgatgccg acattcattc
     1801 tgcccaccca agtgtcatta ttgatgcaga tgccatgttt tctgaagaca ttagctattt
     1861 tggttaccct tcttttcgtc gttcatcact ttccaggcta ggctcatctc gagttctcct
     1921 tcttccctta gagagagact ctgagctgtt gcgtgaacgt gaatccgttt tacgtttacg
     1981 tgaacgaagg tggcttgatg gagcctcatt tgataatgaa aggggttcta ccagcaagga
     2041 aggagagcca aacttggata agaagaatac acctgttcaa agtccagtat ctctaggaga
     2101 agatttgcag tggtggcctg ataaggatgg aacaaaattc atctgtattg gggctctgta
     2161 ttctgaactt ctggctgtca gcagtaaagg agaactttat cagtggaaat ggagtgaatc
     2221 tgagccttac agaaatgccc agaatccttc attacatcat ccacgagcaa catttttggg
     2281 gttaaccaat gaaaagatag tcctcctgtc tgcaaatagc ataagagcaa ctgtagctac
     2341 agaaaataac aaggttgcta catgggtgga tgaaacttta agttctgtgg cttctaaatt
     2401 agagcacact gctcagactt actctgaact tcaaggagag cggatagttt ctttacattg
     2461 ctgtgccctt tacacctgcg ctcagctgga aaacagttta tattggtggg gtgtagttcc
     2521 ttttagtcaa aggaggaaaa tgttagagaa agctagagca aaaaataaaa agcctaaatc
     2581 cagtgctggt atttcttcaa tgccgaacat cactgttggt acccaggtat gcttgagaaa
     2641 taatcctctt tatcatgctg gagcagttgc attttcaatt agtgctggga ttcctaaagt
     2701 tggtgtctta atggagtcag tttggaatat gaatgacagc tgtagatttc aacttagatc
     2761 tcctgaaagc ttgaaaaaca tggaaaaagc tagcaaaact actgaagcta agcctgaaag
     2821 taagcaggag ccagtgaaaa cagaaatggg tcctccacca tctccagcat ccacgtgtag
     2881 tgatgcatcc tcaattgcca gcagtgcatc aatgccatac aaacgacgac ggtcaacccc
     2941 tgcaccaaaa gaagaggaaa aggtgaatga agagcagtgg tctcttcggg aagtggtttt
     3001 tgtggaagat gtcaagaatg ttcctgttgg caaggtgcta aaagtagatg gtgcctatgt
     3061 tgctgtaaaa tttccaggaa cctccagtaa tactaactgt cagaacagct ctggtccaga
     3121 tgctgaccct tcttctctcc tgcaggattg taggttactt agaattgatg aattgcaggt
     3181 tgtcaaaact ggtggaacac cgaaggttcc cgactgtttc caaaggactc ctaaaaagct
     3241 ttgtatacct gaaaaaacag aaatattagc agtgaatgta gattccaaag gtgttcatgc
     3301 tgttctgaag actggaaatt gggtacgata ctgtatcttt gatcttgcta caggaaaagc
     3361 agaacaggaa aataattttc ctacaagcag cattgctttc cttggtcaga atgagaggaa
     3421 tgtagccatt ttcactgctg gacaggaatc tcccattatt cttcgagatg gaaatggtac
     3481 catctaccca atggccaaag attgcatggg aggaataagg gatcccgatt ggctggatct
     3541 tccacctatt agtagtcttg gaatgggtgt gcattcttta ataaatcttc ctgccaattc
     3601 aacaatcaaa aagaaagctg ctgttatcat catggctgta gagaaacaaa ccttaatgca
     3661 acacattctg cgctgtgact atgaggcctg tcgacaatat ctaatgaatc ttgagcaagc
     3721 ggttgtttta gagcagaatc tacagatgct gcagacattc atcagccaca gatgtgatgg
     3781 aaatcgaaat attttgcatg cttgtgtatc agtttgcttt ccaaccagca ataaagaaac
     3841 taaagaagaa gaggaagcgg agcgttctga aagaaataca tttgcagaaa ggctttctgc
     3901 tgttgaggcc attgcaaatg caatatcagt tgtttcaagt aatggcccag gtaatcgggc
     3961 tggatcatca agtagccgaa gtttgagatt acgggaaatg atgagacgtt cgttgagagc
     4021 agctggtttg ggtagacatg aagctggagc ttcatccagt gaccaccagg atccagtttc
     4081 accccccata gctcccccta gttgggttcc tgaccctcct gcgatggatc ctgatggtga
     4141 cattgatttt atcctggccc ccgctgtggg atctcttacc acagcagcaa ccggtactgg
     4201 tcaaggacca agcacctcca ctattccagg tccttccaca gagccatctg tagtagaatc
     4261 caaggatcga aaggcgaatg ctcattttat attgaaattg ttatgtgaca gtgtggttct
     4321 ccagccctat ctacgagaac ttctttctgc caaggatgca agagggatga ccccatttat
     4381 gtcagctgta agtggccgag cttatcctgc tgcaattacc atcttagaaa ctgctcagaa
     4441 aattgcaaaa gctgaaatat cctcaagtga aaaagaggaa gatgtattca tgggaatggt
     4501 ttgcccatca ggtaccaacc ctgatgactc tcctttatat gttttatgtt gtaatgacac
     4561 ttgcagtttt acatggactg gagcagagca cattaaccag gatatttttg agtgtcgaac
     4621 ttgtggcttg ctggagtcac tgtgttgttg tacggaatgt gcaagggttt gtcataaagg
     4681 tcatgattgc aaactcaaac ggacatcacc aacagcctac tgtgattgtt gggagaaatg
     4741 taaatgtaaa actcttattg ctggacagaa atctgctcgt cttgatctac tttatcgcct
     4801 gctcactgct actaatctgg ttactctgcc aaacagcagg ggagagcacc tcttactatt
     4861 cttagtacag acagtcgcaa ggcagacggt ggagcattgt caatacaggc cacctcgaat
     4921 cagggaagat cgtaaccgaa aaacagccag tcctgaagat tcagatatgc cagatcatga
     4981 tttggagcct ccaagatttg cccagcttgc attggagcgt gttctacagg actggaatgc
     5041 cttgaaatct atgattatgt ttgggtcgca ggagaataaa gaccctctta gtgccagcag
     5101 tagaataggc catcttttgc cagaagagca agtatacctc aatcagcaaa gtggcacaat
     5161 tcggctggac tgtttcactc attgccttat agttaagtgt acagcagata ttttgctttt
     5221 agatactcta ctaggtacac tagtgaaaga actccaaaac aaatatacac ctggacgtag
     5281 agaagaagct attgctgtga caatgaggtt tctacgttca gtggcaagag tttttgttat
     5341 tctgagtgtg gaaatggctt catccaaaaa gaaaaacaac tttattccac agccaattgg
     5401 aaaatgcaag cgtgtattcc aagcattgct accttacgct gtggaagaat tgtgcaacgt
     5461 agcagagtca ctgattgttc ctgtcagaat ggggattgct cgtccaactg caccatttac
     5521 cctggctagt actagcatag atgccatgca gggcagtgaa gaattatttt cagtggaacc
     5581 actaccacca cgaccatcat ctgatcagtc tagcagctcc agtcagtctc agtcatccta
     5641 catcatcagg aatccacagc agaggcgcat cagccagtca cagcccgttc ggggcagaga
     5701 tgaagaacag gatgatattg tttcagcaga tgtggaagag gttgaggtgg tggagggtgt
     5761 ggctggagaa gaggatcatc atgatgaaca ggaagaacac ggggaagaaa atgctgaggc
     5821 agagggacaa catgatgagc atgatgaaga cgggagtgat atggagctgg acttgttagc
     5881 agcagctgaa acagaaagtg atagtgaaag taaccacagc aaccaagata atgctagtgg
     5941 gcgcagaagc gttgtcactg cagcaactgc tggttcagaa gcaggagcaa gcagtgttcc
     6001 tgccttcttt tctgaagatg attctcaatc gaatgactca agtgattctg atagcagtag
     6061 tagtcagagt gacgacatag aacaggagac ctttatgctt gatgagccat tagaaagaac
     6121 cacaaatagc tcccatgcca atggtgctgc ccaagctccc cgttcaatgc agtgggctgt
     6181 ccgcaacacc cagcatcagc gagcagccag tacagcccct tccagtacat ctacaccagc
     6241 agcaagttca gcgggtttga tttatattga tccttcaaac ttacgccgga gtggtaccat
     6301 cagtacaagt gctgcagctg cagcagctgc tttggaagct agcaacgcca gcagttacct
     6361 aacatctgca agcagtttag ccagggctta cagcattgtc attagacaaa tctcggactt
     6421 gatgggcctt attcctaagt ataatcacct agtatactct cagattccag cagctgtgaa
     6481 attgacttac caagatgcag taaacttaca gaactatgta gaagaaaagc ttattcccac
     6541 ttggaactgg atggtcagta ttatggattc tactgaagct caattacgtt atggttctgc
     6601 attagcatct gctggtgatc ctggacatcc aaatcatcct cttcacgctt ctcagaattc
     6661 agcgagaaga gagaggatga ctgcgcgaga agaagctagc ttacgaacac ttgaaggcag
     6721 acgacgtgcc accttgctta gcgcccgtca aggaatgatg tctgcacgag gagacttcct
     6781 aaattatgct ctgtctctaa tgcggtctca taatgatgag cattctgatg ttcttccagt
     6841 tttggatgtt tgctcattga agcatgtggc atatgttttt caagcactta tatactggat
     6901 taaggcaatg aatcagcaga caacattgga tacacctcaa ctagaacgca aaaggacgcg
     6961 agaactcttg gaactgggta ttgataatga agattcagaa catgaaaatg atgatgacac
     7021 caatcaaagt gctactttga atgataagga tgatgactct cttcctgcag aaactggcca
     7081 aaaccatcca tttttccgac gttcagactc catgacattc cttgggtgta tacccccaaa
     7141 tccatttgaa gtgcctctgg ctgaagccat ccccttggct gatcagccac atctgttgca
     7201 gccaaatgct agaaaggagg atctttttgg ccgtccaagt cagggtcttt attcttcatc
     7261 tgccagtagt gggaaatgtt taatggaggt tacagtggat agaaactgcc tagaggttct
     7321 tccaacaaaa atgtcttatg ctgccaatct gaaaaatgta atgaacatgc aaaaccggca
     7381 aaaaaaagaa ggggaagaac agcccgtgct gccagaagaa actgagagtt caaaaccagg
     7441 gccatctgct catgatcttg ctgcacaatt aaaaagtagc ttactagcag aaataggact
     7501 tactgaaagt gaagggccac ctctcacatc tttcaggcca cagtgtagct ttatgggaat
     7561 ggttatttcc catgatatgc tgctaggacg ttggcgcctt tctttagaac tgttcggcag
     7621 ggtattcatg gaagatgttg gagcagaacc tggatcaatc ctaactgaat tgggtggttt
     7681 tgaggtaaaa gaatcaaaat tccgcagaga aatggaaaaa ctgagaaacc agcagtcaag
     7741 agatttgtca ctagaggttg atcgggatcg agatcttctc attcagcaga ctatgaggca
     7801 gcttaacaat cactttggtc gaagatgtgc tactacacca atggctgtac acagagtaaa
     7861 agtcacattt aaggatgagc caggagaggg cagtggtgta gcacgaagtt tttatacagc
     7921 cattgcacaa gcatttttat caaatgaaaa attgccaaat ctagagtgta tccaaaatgc
     7981 caacaaaggc acccacacaa gtttaatgca gagattaagg aaccgaggag agagagaccg
     8041 ggaaagggag agagaaaggg aaatgaggag gagtagtggt ttgcgagcag gttctcggag
     8101 ggaccgggat agagacttta gaagacagct ttccatcgac actaggccct ttagaccagc
     8161 ctctgaaggg aatcctagcg atgatcctga gcctttgcca gcacatcggc aggcacttgg
     8221 agagaggctt tatcctcgtg tacaagcaat gcaaccagca tttgcaagta aaatcactgg
     8281 catgttgttg gaattatccc cagctcagct gcttctcctt ctagcaagtg aggattctct
     8341 gagagcaaga gtggatgagg ccatggaact cattattgca catggacggg aaaatggagc
     8401 tgatagtatc ctggatcttg gattagtaga ctcctcagaa aaggtacagc aggaaaaccg
     8461 aaagcgccat ggctctagtc gaagtgtagt agatatggat ttagatgata cagatgatgg
     8521 tgatgacaat gcccctttgt tttaccaacc tgggaaaaga ggattttata ctccaaggcc
     8581 tggcaagaac acagaagcaa ggttgaattg tttcagaaac attggcagga ttcttggact
     8641 atgtctgtta cagaatgaac tctgtcctat cacattgaat agacatgtaa ttaaagtatt
     8701 gcttggtaga aaagtcaatt ggcatgattt tgcttttttt gatcctgtaa tgtatgagag
     8761 tttgcggcaa ctaatcctcg cgtctcagag ttcagatgct gatgctgttt tctcagcaat
     8821 ggatttggca tttgcaattg acctgtgtaa agaagaaggt ggaggacagg ttgaactcat
     8881 tcctaatggt gtaaatatac cagtcactcc acagaatgta tatgagtatg tgcggaaata
     8941 cgcagaacac agaatgttgg tagttgcaga acagccctta catgcaatga ggaaaggtct
     9001 actagatgtg cttccaaaaa attcattaga agatttaacg gcagaagatt ttaggctttt
     9061 ggtaaatggc tgcggtgaag tcaatgtgca aatgctgatc agttttacct ctttcaatga
     9121 tgaatcagga gaaaatgctg agaagcttct gcagttcaag cgttggttct ggtcaatagt
     9181 agagaagatg agcatgacag aacgacaaga tcttgtttac ttttggacat caagcccatc
     9241 actgccagcc agtgaagaag gattccagcc tatgccctca atcacaataa gaccaccaga
     9301 tgaccaacat cttcctactg caaatacttg catttctcga ctttacgtcc cactctattc
     9361 ctctaaacag attctcaaac agaaattgtt actcgccatt aagaccaaga attttggttt
     9421 tgtgtagccg cggacccagc tttcttgtac aaagtggttg atatcggtac cagtcgactc
     9481 tagaggatcc cgggtggcat ccctgtgacc cctccccagt gcctctcctg gccctggaag
     9541 ttgccactcc agtgcccacc agccttgtcc taataaaatt aagttgcatc attttgtctg
     9601 actaggtgtc cttctataat attatggggt ggaggggggt ggtatggagc aaggggcaag
     9661 ttgggaagac aacctgtagg gcctgcgggg tctattggga accaagctgg agtgcagtgg
     9721 cacaatcttg gctcactgca atctccgcct cctgggttca agcgattctc ctgcctcagc
     9781 ctcccgagtt gttgggattc caggcatgca tgaccaggct cagctaattt ttgttttttt
     9841 ggtagagacg gggtttcacc atattggcca ggctggtctc caactcctaa tctcaggtga
     9901 tctacccacc ttggcctccc aaattgctgg gattacaggc gtgaaccact gctcccttcc
     9961 ctgtccttct gattttaaaa taactatacc agcaggagga cgtccagaca cagcataggc
    10021 tacctggcca tgcccaaccg gtgggacatt tgagttgctt gcttggcact gtcctctcat
    10081 gcgttgggtc cactcagtag atgcctgttg aattgggtac gcggccagct tggctgtgga
    10141 atgtgtgtca gttagggtgt ggaaagtccc caggctcccc agcaggcaga agtatgcaaa
    10201 gcatgcatct caattagtca gcaaccaggt gtggaaagtc cccaggctcc ccagcaggca
    10261 gaagtatgca aagcatgcat ctcaattagt cagcaaccat agtcccgccc ctaactccgc
    10321 ccatcccgcc cctaactccg cccagttccg cccattctcc gccccatggc tgactaattt
    10381 tttttattta tgcagaggcc gaggccgcct cggcctctga gctattccag aagtagtgag
    10441 gaggcttttt tggaggccta ggcttttgca aaaagctcct cgaggaactg aaaaaccaga
    10501 aagttaattc cctatagtga gtcgtattaa attcgtaatc atgtcatagc tgtttcctgt
    10561 gtgaaattgt tatccgctca caattccaca caacatacga gccggaagca taaagtgtaa
    10621 agcctggggt gcctaatgag tgagctaact cacattaatt gcgttgcgct cactgcccgc
    10681 tttccagtcg ggaaacctgt cgtgccagct gcattaatga atcggccaac gcgcggggag
    10741 aggcggtttg cgtattgggc gctcttccgc ttcctcgctc actgactcgc tgcgctcggt
    10801 cgttcggctg cggcgagcgg tatcagctca ctcaaaggcg gtaatacggt tatccacaga
    10861 atcaggggat aacgcaggaa agaacatgtg agcaaaaggc cagcaaaagg ccaggaaccg
    10921 taaaaaggcc gcgttgctgg cgtttttcca taggctccgc ccccctgacg agcatcacaa
    10981 aaatcgacgc tcaagtcaga ggtggcgaaa cccgacagga ctataaagat accaggcgtt
    11041 tccccctgga agctccctcg tgcgctctcc tgttccgacc ctgccgctta ccggatacct
    11101 gtccgccttt ctcccttcgg gaagcgtggc gctttctcat agctcacgct gtaggtatct
    11161 cagttcggtg taggtcgttc gctccaagct gggctgtgtg cacgaacccc ccgttcagcc
    11221 cgaccgctgc gccttatccg gtaactatcg tcttgagtcc aacccggtaa gacacgactt
    11281 atcgccactg gcagcagcca ctggtaacag gattagcaga gcgaggtatg taggcggtgc
    11341 tacagagttc ttgaagtggt ggcctaacta cggctacact agaagaacag tatttggtat
    11401 ctgcgctctg ctgaagccag ttaccttcgg aaaaagagtt ggtagctctt gatccggcaa
    11461 acaaaccacc gctggtagcg gtggtttttt tgtttgcaag cagcagatta cgcgcagaaa
    11521 aaaaggatct caagaagatc ctttgatctt ttctacgggg tctgacgctc agtggaacga
    11581 aaactcacgt taagggattt tggtcatgag attatcaaaa aggatcttca cctagatcct
    11641 tttaaattaa aaatgaagtt ttaaatcaat ctaaagtata tatgagtaaa cttggtctga
    11701 cagttaccaa tgcttaatca gtgaggcacc tatctcagcg atctgtctat ttcgttcatc
    11761 catagttgcc tgactccccg tcgtgtagat aactacgata cgggagggct taccatctgg
    11821 ccccagtgct gcaatgatac cgcgagaccc acgctcaccg gctccagatt tatcagcaat
    11881 aaaccagcca gccggaaggg ccgagcgcag aagtggtcct gcaactttat ccgcctccat
    11941 ccagtctatt aattgttgcc gggaagctag agtaagtagt tcgccagtta atagtttgcg
    12001 caacgttgtt gccattgcta caggcatcgt ggtgtcacgc tcgtcgtttg gtatggcttc
    12061 attcagctcc ggttcccaac gatcaaggcg agttacatga tcccccatgt tgtgcaaaaa
    12121 agcggttagc tccttcggtc ctccgatcgt tgtcagaagt aagttggccg cagtgttatc
    12181 actcatggtt atggcagcac tgcataattc tcttactgtc atgccatccg taagatgctt
    12241 ttctgtgact ggtgagtact caaccaagtc attctgagaa tagtgtatgc ggcgaccgag
    12301 ttgctcttgc ccggcgtcaa tacgggataa taccgcgcca catagcagaa ctttaaaagt
    12361 gctcatcatt ggaaaacgtt cttcggggcg aaaactctca aggatcttac cgctgttgag
    12421 atccagttcg atgtaaccca ctcgtgcacc caactgatct tcagcatctt ttactttcac
    12481 cagcgtttct gggtgagcaa aaacaggaag gcaaaatgcc gcaaaaaagg gaataagggc
    12541 gacacggaaa tgttgaatac tcatactctt cctttttcaa tattattgaa gcatttatca
    12601 gggttattgt ctcatgagcg gatacatatt tgaatgtatt tagaaaaata aacaaatagg
    12661 ggttccgcgc acatttcccc gaaaagtgcc acctgacgcg ccctgtagcg gcgcattaag
    12721 cgcggcgggt gtggtggtta cgcgcagcgt gaccgctaca cttgccagcg ccctagcgcc
    12781 cgctcctttc gctttcttcc cttcctttct cgccacgttc gccggctttc cccgtcaagc
    12841 tctaaatcgg gggctccctt tagggttccg atttagtgct ttacggcacc tcgaccccaa
    12901 aaaacttgat tagggtgatg gttcacgtag tgggccatcg ccctgataga cggtttttcg
    12961 ccctttgacg ttggagtcca cgttctttaa tagtggactc ttgttccaaa ctggaacaac
    13021 actcaaccct atctcggtct attcttttga tttataaggg attttgccga tttcggccta
    13081 ttggttaaaa aatgagctga tttaacaaaa atttaacgcg aattttaaca aaatattaac
    13141 gcttacaatt t
//
