LOCUS       sequence-496753-       10102 bp    DNA     circular SYN 09-JAN-2026
DEFINITION  PopuA+40xTetO biosensor cicuit in pNBU2 integrative Bacteroides 
            plasmid.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10102)
  TITLE     Tropini Lab plasmids
REFERENCE   2  (bases 1 to 10102)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Jan 9, 2026 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pJB159
FEATURES             Location/Qualifiers
     source          1..10102
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             207..893
                     /codon_start=1
                     /product="bright cysteine-free monomeric red fluorescent 
                     protein with fast and complete maturation (Gadella et al., 
                     2023)"
                     /label=mScarlet3
                     /translation="MDSTEAVIKEFMRFKVHMEGSMNGHEFEIEGEGEGRPYEGTQTAK
                     LKVTKGGPLPFSWDILSPQFMYGSRAFIKHPADIPDYWKQSFPEGFKWERVMIFEDGGT
                     VSVTQDTSLEDGTLIYKVKLRGGNFPPDGPVMQKRTMGWEASTERLYPEDVVLKGDIKM
                     ALRLKDGGRYLADFKTTYKAKKPVQMPGAFNIDRKLDITSHNEDYTVVEQYERSVARHS
                     TGGSGGS"
     primer_bind     complement(337..355)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     terminator      919..946
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     protein_bind    1023..1041
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    1070..1088
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    1119..1137
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    1151..1169
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    1193..1211
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    1239..1257
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    1281..1299
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    1329..1347
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    1378..1396
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    1420..1438
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    1483..1501
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    1530..1548
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    1579..1597
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    1611..1629
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    1653..1671
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    1699..1717
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    1741..1759
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    1789..1807
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    1838..1856
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    1880..1898
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    1943..1961
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    1990..2008
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2039..2057
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2071..2089
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2113..2131
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2159..2177
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2201..2219
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2249..2267
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2298..2316
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2340..2358
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2403..2421
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2450..2468
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2499..2517
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2531..2549
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2573..2591
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2619..2637
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2661..2679
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2709..2727
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2758..2776
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2800..2818
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     terminator      2853..2924
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     terminator      2940..2967
                     /label=T7Te terminator
                     /note="phage T7 early transcription terminator"
     CDS             3505..4128
                     /codon_start=1
                     /gene="tetR from transposon Tn10"
                     /product="tetracycline repressor TetR"
                     /label=TetR
                     /note="TetR binds to the tetracycline operator tetO to 
                     inhibit transcription. This inhibition can be relieved by 
                     adding tetracycline or doxycycline."
                     /translation="MSRLDKSKVINSALELLNEVGIEGLTTRKLAQKLGVEQPTLYWHV
                     KNKRALLDALAIEMLDRHHTHFCPLEGESWQDFLRNNAKSFRCALLSHRDGAKVHLGTR
                     PTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEDQEHQVAKEERETPTT
                     DSMPPLLRQAIELFDHQGAEPAFLFGLELIICGLEKQLKCESGS"
     primer_bind     complement(3578..3597)
                     /label=Tet-R
                     /note="Tetracycline resistance gene, reverse primer"
     protein_bind    4293..4311
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     CDS             join(4398..4400,4401..4403,4404..5117)
                     /codon_start=1
                     /product="monomeric GFP derivative that displays 
                     exceptionally high brightness in cells (Campbell et al., 
                     2020)"
                     /label=mGreenLantern
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVRGEGEGDATNGKLTL
                     KFICTTGKLPVPWPTLVTTLGYGVACFARYPDHMKQHDFFKSAMPEGYVQERTISFKDD
                     GTYKTRAEVKFEGDTLVNRIVLKGIDFKEDGNILGHKLEYNFNSHKVYITADKQKNGIK
                     ANFKTRHNVEDGGVQLADHYQQNTPIGDGPVLLPDNHYLSHQSKLSKDPNEKRDHMVLK
                     ERVTAAGITHDMDELYK"
     primer_bind     complement(4443..4464)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     terminator      5138..5165
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     CDS             complement(join(7145..7936,7937..8005))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     7768..7787
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      9297..9685
                     /label=R6K gamma ori
                     /note="gamma replication origin from E. coli plasmid R6K; 
                     requires the R6K initiator protein pi for replication"
     oriT            9856..9965
                     /note="incP origin of transfer"
ORIGIN
        1 caattgggct accttttttt tgttttgttt gcaatggtta atctattgtt aaaatttaaa
       61 gtttcacttg aactttcaaa taatgttctt atatttgcag tgtcgaaaga aacaaagtag
      121 gactgatcac acaacattta aaaaataaca ttatgaaagc actcgaaaag agattcaaag
      181 gtaatattaa caataattta ttttcaatgg atagcaccga ggcagtgatc aaggagttca
      241 tgcggttcaa ggtgcacatg gagggctcca tgaacggcca cgagttcgag atcgagggcg
      301 agggcgaggg ccgcccctac gagggcaccc agaccgccaa gctgaaggtg accaagggtg
      361 gccccctgcc cttctcctgg gacatcctgt cccctcagtt catgtacggc tccagggcct
      421 tcatcaagca ccccgccgac atccccgact actggaagca gtccttcccc gagggcttca
      481 agtgggagcg cgtgatgatc ttcgaggacg gcggcaccgt gtccgtgacc caggacacct
      541 ccctggagga cggcaccctg atctacaagg tgaagctccg cggcggcaac ttccctcctg
      601 acggccccgt aatgcagaag aggacaatgg gctgggaagc atccaccgag cggttgtacc
      661 ccgaggacgt cgtgctgaag ggcgacatta agatggccct gcgcctgaag gacggcggcc
      721 gctacctggc ggacttcaag accacctaca aggccaagaa gcccgtgcag atgcccggcg
      781 ccttcaacat cgaccgcaag ttggacatca cctcccacaa cgaggactac accgtggtgg
      841 aacagtacga acgctccgtg gcccgccact ccaccggcgg ctccggtggc tcctaataaa
      901 tcctagctga ttagctcgag aaggccatcc tgacggatgg cctttttttt gtatggggac
      961 tctgagaacc ctacctttca aaccccgagc ggccgcttct agagatcgat tttagagctg
     1021 cctccctatc agtgatagag attgctacaa ctgcaaggta gtcccggtat ccctatcagt
     1081 gatagagact gatagctacg agtctgagat cctacttgtc cctatcagtg atagagacag
     1141 taccgagttt tccctatcag tgatagagag ctcatactgc gacagagtct tttccctatc
     1201 agtgatagag ataggctcct ttgtcaaaag tgatcgcttc cctatcagtg atagagactt
     1261 cacgggataa tgtgttctac tccctatcag tgatagagag cttaagtcaa tccaatgcgt
     1321 tcaggtgctc cctatcagtg atagagaaac tccgtcatcg tggatgtcaa tactgactcc
     1381 ctatcagtga tagagattga tttcaccgct ggcgtcaatt ccctatcagt gatagagact
     1441 taggtgtccc tagaattact agagatcgat tttagagctg cctccctatc agtgatagag
     1501 attgctacaa ctgcaaggta gtcccggtat ccctatcagt gatagagact gatagctacg
     1561 agtctgagat cctacttgtc cctatcagtg atagagacag taccgagttt tccctatcag
     1621 tgatagagag ctcatactgc gacagagtct tttccctatc agtgatagag ataggctcct
     1681 ttgtcaaaag tgatcgcttc cctatcagtg atagagactt cacgggataa tgtgttctac
     1741 tccctatcag tgatagagag cttaagtcaa tccaatgcgt tcaggtgctc cctatcagtg
     1801 atagagaaac tccgtcatcg tggatgtcaa tactgactcc ctatcagtga tagagattga
     1861 tttcaccgct ggcgtcaatt ccctatcagt gatagagact taggtgtccc tagaattact
     1921 agagatcgat tttagagctg cctccctatc agtgatagag attgctacaa ctgcaaggta
     1981 gtcccggtat ccctatcagt gatagagact gatagctacg agtctgagat cctacttgtc
     2041 cctatcagtg atagagacag taccgagttt tccctatcag tgatagagag ctcatactgc
     2101 gacagagtct tttccctatc agtgatagag ataggctcct ttgtcaaaag tgatcgcttc
     2161 cctatcagtg atagagactt cacgggataa tgtgttctac tccctatcag tgatagagag
     2221 cttaagtcaa tccaatgcgt tcaggtgctc cctatcagtg atagagaaac tccgtcatcg
     2281 tggatgtcaa tactgactcc ctatcagtga tagagattga tttcaccgct ggcgtcaatt
     2341 ccctatcagt gatagagact taggtgtccc tagaattact agagatcgat tttagagctg
     2401 cctccctatc agtgatagag attgctacaa ctgcaaggta gtcccggtat ccctatcagt
     2461 gatagagact gatagctacg agtctgagat cctacttgtc cctatcagtg atagagacag
     2521 taccgagttt tccctatcag tgatagagag ctcatactgc gacagagtct tttccctatc
     2581 agtgatagag ataggctcct ttgtcaaaag tgatcgcttc cctatcagtg atagagactt
     2641 cacgggataa tgtgttctac tccctatcag tgatagagag cttaagtcaa tccaatgcgt
     2701 tcaggtgctc cctatcagtg atagagaaac tccgtcatcg tggatgtcaa tactgactcc
     2761 ctatcagtga tagagattga tttcaccgct ggcgtcaatt ccctatcagt gatagagact
     2821 taggtgtccc tagaattact agagccaggc atcaaataaa acgaaaggct cagtcgaaag
     2881 actgggcctt tcgttttatc tgttgtttgt cggtgaacgc tctctactag agtcacactg
     2941 gctcaccttc gggtgggcct ttctgcgttt atatatgatg tgtaatgaca attatccttc
     3001 cccaggaaaa ttgtaatagc cctgttgaac atataaatct agtttagtta ttgacgccaa
     3061 atatacggtg tttaagtgaa atattcaaac ttattttata tttatctgcc ggtttgttct
     3121 attttgctga gtgatagggg aatattaccc ggaaactttc catatcatta caaataaagc
     3181 cctctgtatg ccccggattt tgaatataat ttgtatgttt aatccgtttt ttgtatcttt
     3241 gtgccagcaa gatactggaa caggctgttt ttatacataa aaatgctatt ttttgctatt
     3301 cgttgaaagg attatcttat caaattctat tcctttttta attactcagt ctgtcaataa
     3361 tcattttatt aattatttcg ggattcattg ttatttgtcc cttatttaaa tcagaacttt
     3421 atacgatata aagaaagatt cctgttatcg tttgttcttg tcttgctgtg tatattattt
     3481 taataaccta aaccatttta ttgtatgtcc agattagata aaagtaaagt gattaacagc
     3541 gcattagagc tgcttaatga ggtcggaatc gaaggtttaa caacccgtaa actcgcccag
     3601 aagctaggtg tagagcagcc tacattgtat tggcatgtaa aaaataagcg ggctttgctc
     3661 gacgccttag ccattgagat gttagatagg caccatactc acttttgccc tttagaaggg
     3721 gaaagctggc aagatttttt acgtaataac gctaaaagtt ttagatgtgc tttactaagt
     3781 catcgcgatg gagcaaaagt acatttaggt acacggccta cagaaaaaca gtatgaaact
     3841 ctcgaaaatc aattagcctt tttatgccaa caaggttttt cactagagaa tgcattatat
     3901 gcactcagcg ctgtggggca ttttacttta ggttgcgtat tggaagatca agagcatcaa
     3961 gtcgctaaag aagaaaggga aacacctact actgatagta tgccgccatt attacgacaa
     4021 gctatcgaat tatttgatca ccaaggtgca gagccagcct tcttattcgg ccttgaattg
     4081 atcatatgcg gattagaaaa acaacttaaa tgtgaaagtg ggtcttaatg gccacatcgg
     4141 ttcctgctag aaggcccatc ctgacggatg gccttttggt attcgagcat tacctcatac
     4201 tttggatttt tgttttgttt gcaatggtta atctattgtt aaaatttaaa gtttcacttg
     4261 aactttcaaa taatgttctt atatttgcag tgtccctatc agtgatagag agtaggatca
     4321 cacaacattt aaaaaataac attatgaaag cactcgaaaa gagattcaaa ggtaatatta
     4381 acaataattt attttcaatg gtgagcaagg gcgaggagct gttcaccggg gtggtgccca
     4441 tcctggtcga gctggacggc gacgtaaacg gccacaagtt cagcgtccgc ggcgagggcg
     4501 agggcgatgc caccaacggc aagctgaccc tgaagttcat ctgcaccacc ggcaagctgc
     4561 ccgtgccctg gcccaccctc gtgaccacct taggctacgg cgtggcctgc ttcgcccgct
     4621 accccgacca catgaagcag cacgacttct tcaagtccgc catgcccgaa ggctacgtcc
     4681 aggagcgcac catctctttc aaggacgacg gtacctacaa gacccgcgcc gaggtgaagt
     4741 tcgagggcga caccctggtg aaccgcatcg tgctgaaggg catcgacttc aaggaggacg
     4801 gcaacatcct ggggcacaag ctggagtaca acttcaacag ccacaaggtc tatatcacgg
     4861 ccgacaagca gaagaacggc atcaaggcta acttcaagac ccgccacaac gttgaggacg
     4921 gcggcgtgca gctcgccgac cactaccagc agaacacccc catcggcgac ggccccgtgc
     4981 tgctgcccga caaccactac ctgagccatc agtccaagct gagcaaagac cccaacgaga
     5041 agcgcgatca catggtcctg aaggagaggg tgaccgccgc cgggattaca catgacatgg
     5101 acgagctgta caagtaatag cgttcagctc gactgtgaga aggccatcct gacggatggc
     5161 cttttttttt cgttataact agttgcgttc ctagccgcta ctagtgtaat tgcctatctt
     5221 ccagtgatgg aacagcattt gtgcattggc tgcaacaatc agccttactt gtgcctgttc
     5281 tatttccgaa ccgaccgctt gtatgaatgc atcaaaattc gttttctcta cgttggattc
     5341 cttgttgctc atattgtgat gataatttct acaaatatag tcattggtaa ctatctatga
     5401 aactgtttga tacttttata gttgattaaa cttgttcatg gcatttgcct taatatcatc
     5461 cgctatgtca atgtagggtt tcatagcttt gtagtcgctg tgtcccgtcc atttcatgac
     5521 cacctgtgcc gggattccga gagccagcgc attgcagatg aatgtccttt ttcctgcatg
     5581 ggtactgagc aaagcgtatt tgtgtgtgac ttcatcaata cgttcatttc ccttgtagta
     5641 ggtttcccgt acaggctcgt tgatttctgc cagttcgccc agctctttca ggtaatcgtt
     5701 catcttctgg ttgctgatga cgggcagagc catgtaattc tcgaaatgga tgtccttgta
     5761 tttgtccagt atggctttgc tgtatttgtt cagttcaatc gtcaggctgt cggcagtctt
     5821 gactgtggtt atttcgatgt ggtcggactt cacatcgctt cttttcagat tgcgaacatc
     5881 cgaataccgc aaactcgtaa agcagcagaa caggaaaaca tcacgcacac gttccaggta
     5941 ttgcttatcc ttgggtatct ggtagtcttt cagcttgttc agttcatccc aagtcaggaa
     6001 gattactttt ttcgaggtgg ttttcagttt cggtttgaac gtatcgtatg caatgttctg
     6061 atgatgtcct ttcttgaagc tccagcgcag gaaccatttg aggaatccca tttgcttgcc
     6121 gatggtgctg tttctcatat ccttggtgtc acgcaggaag ttgacgtatt cgttcaatcc
     6181 aaactcgttg aaatagttga acgttgcatc ctccttgaac tctttgaggt ggttcctcac
     6241 tgctgcaaat ttttcatagg tggatgccgt ccagttattc tggttaccgc actcttttac
     6301 aaactcatcg aacacctccc aaaagctgac aggggcttct tccggctgtt cttcactggt
     6361 gtctttcatt ctcatgttga aagcttcctt caactgttgg gtcgttggca tgacctcctg
     6421 cacctcaaat tccttgaaaa tattctggat ttcggcatag tatttcagca agtccgtatt
     6481 gatttcggct gcactttgct ttagcttgtt ggtacatccg ttctttaccc gctgcttatc
     6541 tgcatcccat ttggctacgt caatccggta gcccgttgta aactcgatac gttggctggc
     6601 aaagatgaca cgcatacgga tgggtacgtt ctctacgatt ggcacaccgt tctttttccg
     6661 gctctccaat gcaaaaatga tgttgcgctt gatattcata attgggtgcg tttgaaattc
     6721 tacacccaaa tatacaccca attattgaga tagcaaaaga catttagaaa catttacttt
     6781 tactctatat tgtaatttac acttgattat cagtcgtttg cagttttatg atattctgtg
     6841 aaagtataag ttcgagagcc tgtctctccg caaaaaacgc tgaaaatcag cagattgcaa
     6901 aacaaacacc ctgttttaca cccaagaatg taaagtcggg tgtttttgtt ttatttaaga
     6961 taatacaacc actacataat aaaagagtag cgatattaaa agaatccgat gagaaaagac
     7021 taatatttat ctatccattc agtttgattt ctcaggactt tacatcgtcc tgaaagtatt
     7081 tgttggtacc ggtaccgagg acgcgacagc tgcagacgcg ttatgagtaa acttggtctg
     7141 acagttacca atgcttaatc agtgaggcac ctatctcagc gatctgtcta tttcgttcat
     7201 ccatagttgc ctgactcccc gtcgtgtaga taactacgat acgggagggc ttaccatctg
     7261 gccccagtgc tgcaatgata ccgcgggacc cacgctcacc ggctccagat ttatcagcaa
     7321 taaaccagcc agccggaagg gccgagcgca gaagtggtcc tgcaacttta tccgcctcca
     7381 tccagtctat taattgttgc cgggaagcta gagtaagtag ttcgccagtt aatagtttgc
     7441 gcaacgttgt tgccattgct acaggcatcg tggtgtcacg ctcgtcgttt ggtatggctt
     7501 cattcagctc cggttcccaa cgatcaaggc gagttacatg atcccccatg ttgtgcaaaa
     7561 aagcggttag ctccttcggt cctccgatcg ttgtcagaag taagttggcc gcagtgttat
     7621 cactcatggt tatggcagca ctgcataatt ctcttactgt catgccatcc gtaagatgct
     7681 tttctgtgac tggtgagtac tcaaccaagt cattctgaga atagtgtatg cggcgaccga
     7741 gttgctcttg cccggcgtca atacgggata ataccgcgcc acatagcaga actttaaaag
     7801 tgctcatcat tggaaaacgt tcttcggggc gaaaactctc aaggatctta ccgctgttga
     7861 gatccagttc gatgtaaccc actcgtgcac ccaactgatc ttcagcatct tttactttca
     7921 ccagcgtttc tgggtgagca aaaacaggaa ggcaaaatgc cgcaaaaaag ggaataaggg
     7981 cgacacggaa atgttgaata ctcatactct tcctttttca atcatggccg cgggattaaa
     8041 agtcggggat tggtgaacaa aaaggtgttt ctctctttaa gagaaatatc gttttgctaa
     8101 acagttgata ttgaggtatc attttatcgt aaaagacatt tttgctcaac aattgcttga
     8161 cggaaatcaa caaattttag cattttgtaa aaaagtcgct atataatttg gtgaattgga
     8221 gttattttca tatttttgca tcccgaagag tttctcttaa agagagaaac atcttttgca
     8281 taccttttcc gaccgaattt ttatgtcgta aagaggggct ttgcaggggg tggactcaga
     8341 aagatgagaa tagatgacta ttgtagttga aacacataga aagttgctga tatacagacc
     8401 gatacgcata tcgggatgaa ccatgagtac gttcttttct caaaaaacat aaatattcga
     8461 aaagagatgc aataaattaa ggagaggtta taatgaacaa agtaaatata aaagatagtc
     8521 aaaattttat tacttcaaaa tatcacatag aaaaaataat gaattgcata agtttagatg
     8581 aaaaagataa catctttgaa ataggtgcag ggaaaggtca ttttactgct ggattggtaa
     8641 agagatgtaa ttttgtaacg gcgatagaaa ttgattctaa attatgtgag gtaactcgta
     8701 ataagctctt aaattatcct aactatcaaa tagtaaatga tgatatactg aaatttacat
     8761 ttcctagcca caatccatat aaaatatttg gcaacatacc ttacaacata agcacaaata
     8821 taattcgaaa aattgttttt gaaagttcag ccacaataag ttatttaata gtggaatatg
     8881 gttttgctaa aatgttatta gatacaaaca gatcactagc attgctgtta atggcagagg
     8941 tagatatttc tatattagca aaaattccta ggtattattt ccatccaaaa cctaaagtgg
     9001 atagcacatt aattgtatta aaaagaaagc cagcaaaaat ggcatttaaa gagagaaaaa
     9061 aatatgaaac ttttgtaatg aaatgggtta acaaagagta cgaaaaactg tttacaaaaa
     9121 atcaatttaa taaagcttta aaacatgcga gaatatatga tataaacaat attagtttcg
     9181 aacaatttgt atcgctattt aatagttata aaatatttaa cggctaaaaa caataggcca
     9241 catgcaactg taaatgttta cgcgggtacc gacaccgcgg tggaggggaa ttcccatgtc
     9301 agccgttaag tgttcctgtg tcactcaaaa ttgctttgag aggctctaag ggcttctcag
     9361 tgcgttacat ccctggcttg ttgtccacaa ccgttaaacc ttaaaagctt taaaagcctt
     9421 atatattctt ttttttctta taaaacttaa aaccttagag gctatttaag ttgctgattt
     9481 atattaattt tattgttcaa acatgagagc ttagtacgtg aaacatgaga gcttagtacg
     9541 ttagccatga gagcttagta cgttagccat gagggtttag ttcgttaaac atgagagctt
     9601 agtacgttaa acatgagagc ttagtacgtg aaacatgaga gcttagtacg tactatcaac
     9661 aggttgaact gctgatcttc agatcctcta cgccggacgc atcgtggccg gatcaattcc
     9721 gttttccgct gcataaccct gcttcggggt cattatagcg attttttcgg tatatccatc
     9781 ctttttcgca cgatatacag gattttgcca aagggttcgt gtagactttc cttggtgtat
     9841 ccaacggcgt cagccgggca ggataggtga agtaggccca cccgcgagcg ggtgttcctt
     9901 cttcactgtc ccttattcgc acctggcggt gctcaacggg aatcctgctc tgcgaggctg
     9961 gccggctacc gccggcgtaa cagatgaggg caagcggatg gctgatgaaa ccaagccaac
    10021 caggaagggc agcccaccta tcacggaatt gatccccctc gaattggaat tctagcctgg
    10081 aatagggaag caccacatca cc
//
