LOCUS       sequence-496754-       10062 bp    DNA     circular SYN 09-JAN-2026
DEFINITION  PBT0779+40xTetO biosensor cicuit in pNBU2 integrative Bacteroides 
            plasmid.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10062)
  TITLE     Tropini Lab plasmids
REFERENCE   2  (bases 1 to 10062)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Jan 9, 2026 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pJB178
FEATURES             Location/Qualifiers
     source          1..10062
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             207..893
                     /codon_start=1
                     /product="bright cysteine-free monomeric red fluorescent 
                     protein with fast and complete maturation (Gadella et al., 
                     2023)"
                     /label=mScarlet3
                     /translation="MDSTEAVIKEFMRFKVHMEGSMNGHEFEIEGEGEGRPYEGTQTAK
                     LKVTKGGPLPFSWDILSPQFMYGSRAFIKHPADIPDYWKQSFPEGFKWERVMIFEDGGT
                     VSVTQDTSLEDGTLIYKVKLRGGNFPPDGPVMQKRTMGWEASTERLYPEDVVLKGDIKM
                     ALRLKDGGRYLADFKTTYKAKKPVQMPGAFNIDRKLDITSHNEDYTVVEQYERSVARHS
                     TGGSGGS"
     primer_bind     complement(337..355)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     terminator      919..946
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     protein_bind    1023..1041
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    1070..1088
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    1119..1137
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    1151..1169
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    1193..1211
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    1239..1257
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    1281..1299
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    1329..1347
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    1378..1396
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    1420..1438
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    1483..1501
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    1530..1548
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    1579..1597
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    1611..1629
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    1653..1671
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    1699..1717
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    1741..1759
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    1789..1807
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    1838..1856
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    1880..1898
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    1943..1961
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    1990..2008
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2039..2057
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2071..2089
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2113..2131
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2159..2177
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2201..2219
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2249..2267
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2298..2316
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2340..2358
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2403..2421
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2450..2468
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2499..2517
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2531..2549
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2573..2591
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2619..2637
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2661..2679
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2709..2727
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2758..2776
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2800..2818
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     terminator      2853..2924
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     terminator      2940..2967
                     /label=T7Te terminator
                     /note="phage T7 early transcription terminator"
     CDS             3465..4088
                     /codon_start=1
                     /gene="tetR from transposon Tn10"
                     /product="tetracycline repressor TetR"
                     /label=TetR
                     /note="TetR binds to the tetracycline operator tetO to 
                     inhibit transcription. This inhibition can be relieved by 
                     adding tetracycline or doxycycline."
                     /translation="MSRLDKSKVINSALELLNEVGIEGLTTRKLAQKLGVEQPTLYWHV
                     KNKRALLDALAIEMLDRHHTHFCPLEGESWQDFLRNNAKSFRCALLSHRDGAKVHLGTR
                     PTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEDQEHQVAKEERETPTT
                     DSMPPLLRQAIELFDHQGAEPAFLFGLELIICGLEKQLKCESGS"
     primer_bind     complement(3538..3557)
                     /label=Tet-R
                     /note="Tetracycline resistance gene, reverse primer"
     protein_bind    4253..4271
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     CDS             join(4358..4360,4361..4363,4364..5077)
                     /codon_start=1
                     /product="monomeric GFP derivative that displays 
                     exceptionally high brightness in cells (Campbell et al., 
                     2020)"
                     /label=mGreenLantern
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVRGEGEGDATNGKLTL
                     KFICTTGKLPVPWPTLVTTLGYGVACFARYPDHMKQHDFFKSAMPEGYVQERTISFKDD
                     GTYKTRAEVKFEGDTLVNRIVLKGIDFKEDGNILGHKLEYNFNSHKVYITADKQKNGIK
                     ANFKTRHNVEDGGVQLADHYQQNTPIGDGPVLLPDNHYLSHQSKLSKDPNEKRDHMVLK
                     ERVTAAGITHDMDELYK"
     primer_bind     complement(4403..4424)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     terminator      5098..5125
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     CDS             complement(join(7105..7896,7897..7965))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     7728..7747
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      9257..9645
                     /label=R6K gamma ori
                     /note="gamma replication origin from E. coli plasmid R6K; 
                     requires the R6K initiator protein pi for replication"
     oriT            9816..9925
                     /note="incP origin of transfer"
ORIGIN
        1 caattgggct accttttttt tgttttgttt gcaatggtta atctattgtt aaaatttaaa
       61 gtttcacttg aactttcaaa taatgttctt atatttgcag tgtcgaaaga aacaaagtag
      121 gactgatcac acaacattta aaaaataaca ttatgaaagc actcgaaaag agattcaaag
      181 gtaatattaa caataattta ttttcaatgg atagcaccga ggcagtgatc aaggagttca
      241 tgcggttcaa ggtgcacatg gagggctcca tgaacggcca cgagttcgag atcgagggcg
      301 agggcgaggg ccgcccctac gagggcaccc agaccgccaa gctgaaggtg accaagggtg
      361 gccccctgcc cttctcctgg gacatcctgt cccctcagtt catgtacggc tccagggcct
      421 tcatcaagca ccccgccgac atccccgact actggaagca gtccttcccc gagggcttca
      481 agtgggagcg cgtgatgatc ttcgaggacg gcggcaccgt gtccgtgacc caggacacct
      541 ccctggagga cggcaccctg atctacaagg tgaagctccg cggcggcaac ttccctcctg
      601 acggccccgt aatgcagaag aggacaatgg gctgggaagc atccaccgag cggttgtacc
      661 ccgaggacgt cgtgctgaag ggcgacatta agatggccct gcgcctgaag gacggcggcc
      721 gctacctggc ggacttcaag accacctaca aggccaagaa gcccgtgcag atgcccggcg
      781 ccttcaacat cgaccgcaag ttggacatca cctcccacaa cgaggactac accgtggtgg
      841 aacagtacga acgctccgtg gcccgccact ccaccggcgg ctccggtggc tcctaataaa
      901 tcctagctga ttagctcgag aaggccatcc tgacggatgg cctttttttt gtatggggac
      961 tctgagaacc ctacctttca aaccccgagc ggccgcttct agagatcgat tttagagctg
     1021 cctccctatc agtgatagag attgctacaa ctgcaaggta gtcccggtat ccctatcagt
     1081 gatagagact gatagctacg agtctgagat cctacttgtc cctatcagtg atagagacag
     1141 taccgagttt tccctatcag tgatagagag ctcatactgc gacagagtct tttccctatc
     1201 agtgatagag ataggctcct ttgtcaaaag tgatcgcttc cctatcagtg atagagactt
     1261 cacgggataa tgtgttctac tccctatcag tgatagagag cttaagtcaa tccaatgcgt
     1321 tcaggtgctc cctatcagtg atagagaaac tccgtcatcg tggatgtcaa tactgactcc
     1381 ctatcagtga tagagattga tttcaccgct ggcgtcaatt ccctatcagt gatagagact
     1441 taggtgtccc tagaattact agagatcgat tttagagctg cctccctatc agtgatagag
     1501 attgctacaa ctgcaaggta gtcccggtat ccctatcagt gatagagact gatagctacg
     1561 agtctgagat cctacttgtc cctatcagtg atagagacag taccgagttt tccctatcag
     1621 tgatagagag ctcatactgc gacagagtct tttccctatc agtgatagag ataggctcct
     1681 ttgtcaaaag tgatcgcttc cctatcagtg atagagactt cacgggataa tgtgttctac
     1741 tccctatcag tgatagagag cttaagtcaa tccaatgcgt tcaggtgctc cctatcagtg
     1801 atagagaaac tccgtcatcg tggatgtcaa tactgactcc ctatcagtga tagagattga
     1861 tttcaccgct ggcgtcaatt ccctatcagt gatagagact taggtgtccc tagaattact
     1921 agagatcgat tttagagctg cctccctatc agtgatagag attgctacaa ctgcaaggta
     1981 gtcccggtat ccctatcagt gatagagact gatagctacg agtctgagat cctacttgtc
     2041 cctatcagtg atagagacag taccgagttt tccctatcag tgatagagag ctcatactgc
     2101 gacagagtct tttccctatc agtgatagag ataggctcct ttgtcaaaag tgatcgcttc
     2161 cctatcagtg atagagactt cacgggataa tgtgttctac tccctatcag tgatagagag
     2221 cttaagtcaa tccaatgcgt tcaggtgctc cctatcagtg atagagaaac tccgtcatcg
     2281 tggatgtcaa tactgactcc ctatcagtga tagagattga tttcaccgct ggcgtcaatt
     2341 ccctatcagt gatagagact taggtgtccc tagaattact agagatcgat tttagagctg
     2401 cctccctatc agtgatagag attgctacaa ctgcaaggta gtcccggtat ccctatcagt
     2461 gatagagact gatagctacg agtctgagat cctacttgtc cctatcagtg atagagacag
     2521 taccgagttt tccctatcag tgatagagag ctcatactgc gacagagtct tttccctatc
     2581 agtgatagag ataggctcct ttgtcaaaag tgatcgcttc cctatcagtg atagagactt
     2641 cacgggataa tgtgttctac tccctatcag tgatagagag cttaagtcaa tccaatgcgt
     2701 tcaggtgctc cctatcagtg atagagaaac tccgtcatcg tggatgtcaa tactgactcc
     2761 ctatcagtga tagagattga tttcaccgct ggcgtcaatt ccctatcagt gatagagact
     2821 taggtgtccc tagaattact agagccaggc atcaaataaa acgaaaggct cagtcgaaag
     2881 actgggcctt tcgttttatc tgttgtttgt cggtgaacgc tctctactag agtcacactg
     2941 gctcaccttc gggtgggcct ttctgcgttt atatatgatg gcagcatcca ttgcctgaaa
     3001 aataaccaga accgagtagc ggtcttgtgc ataaagcata ctttgcagtt ctgacagctt
     3061 ttcgatattt tgggctaact gttctttggc atcctgtttg gacatatcag cagtaaatga
     3121 gggattgaaa tcggatacat gatgttcctt ccccggtttg gctgtaagct cttttaagac
     3181 ttcttttttc attgttgtat aattaggttg ttataggtat ataacagttg aataaagaaa
     3241 tcagttcttg ccaaattaag agcgttgaat agtaattccc caattatgta tcaacagatt
     3301 ctacataatt ccacactaat tccacaatta tatttatatt gtctgctgtt tatatgtttg
     3361 attatcagtt atatatggcg gagtgaaacg tttggcatct gctttgctat atattaagta
     3421 caagaaacaa tagttctatt attcacactt aaaaaattac gattatgtcc agattagata
     3481 aaagtaaagt gattaacagc gcattagagc tgcttaatga ggtcggaatc gaaggtttaa
     3541 caacccgtaa actcgcccag aagctaggtg tagagcagcc tacattgtat tggcatgtaa
     3601 aaaataagcg ggctttgctc gacgccttag ccattgagat gttagatagg caccatactc
     3661 acttttgccc tttagaaggg gaaagctggc aagatttttt acgtaataac gctaaaagtt
     3721 ttagatgtgc tttactaagt catcgcgatg gagcaaaagt acatttaggt acacggccta
     3781 cagaaaaaca gtatgaaact ctcgaaaatc aattagcctt tttatgccaa caaggttttt
     3841 cactagagaa tgcattatat gcactcagcg ctgtggggca ttttacttta ggttgcgtat
     3901 tggaagatca agagcatcaa gtcgctaaag aagaaaggga aacacctact actgatagta
     3961 tgccgccatt attacgacaa gctatcgaat tatttgatca ccaaggtgca gagccagcct
     4021 tcttattcgg ccttgaattg atcatatgcg gattagaaaa acaacttaaa tgtgaaagtg
     4081 ggtcttaatg gccacatcgg ttcctgctag aaggcccatc ctgacggatg gccttttggt
     4141 attcgagcat tacctcatac tttggatttt tgttttgttt gcaatggtta atctattgtt
     4201 aaaatttaaa gtttcacttg aactttcaaa taatgttctt atatttgcag tgtccctatc
     4261 agtgatagag agtaggatca cacaacattt aaaaaataac attatgaaag cactcgaaaa
     4321 gagattcaaa ggtaatatta acaataattt attttcaatg gtgagcaagg gcgaggagct
     4381 gttcaccggg gtggtgccca tcctggtcga gctggacggc gacgtaaacg gccacaagtt
     4441 cagcgtccgc ggcgagggcg agggcgatgc caccaacggc aagctgaccc tgaagttcat
     4501 ctgcaccacc ggcaagctgc ccgtgccctg gcccaccctc gtgaccacct taggctacgg
     4561 cgtggcctgc ttcgcccgct accccgacca catgaagcag cacgacttct tcaagtccgc
     4621 catgcccgaa ggctacgtcc aggagcgcac catctctttc aaggacgacg gtacctacaa
     4681 gacccgcgcc gaggtgaagt tcgagggcga caccctggtg aaccgcatcg tgctgaaggg
     4741 catcgacttc aaggaggacg gcaacatcct ggggcacaag ctggagtaca acttcaacag
     4801 ccacaaggtc tatatcacgg ccgacaagca gaagaacggc atcaaggcta acttcaagac
     4861 ccgccacaac gttgaggacg gcggcgtgca gctcgccgac cactaccagc agaacacccc
     4921 catcggcgac ggccccgtgc tgctgcccga caaccactac ctgagccatc agtccaagct
     4981 gagcaaagac cccaacgaga agcgcgatca catggtcctg aaggagaggg tgaccgccgc
     5041 cgggattaca catgacatgg acgagctgta caagtaatag cgttcagctc gactgtgaga
     5101 aggccatcct gacggatggc cttttttttt cgttataact agttgcgttc ctagccgcta
     5161 ctagtgtaat tgcctatctt ccagtgatgg aacagcattt gtgcattggc tgcaacaatc
     5221 agccttactt gtgcctgttc tatttccgaa ccgaccgctt gtatgaatgc atcaaaattc
     5281 gttttctcta cgttggattc cttgttgctc atattgtgat gataatttct acaaatatag
     5341 tcattggtaa ctatctatga aactgtttga tacttttata gttgattaaa cttgttcatg
     5401 gcatttgcct taatatcatc cgctatgtca atgtagggtt tcatagcttt gtagtcgctg
     5461 tgtcccgtcc atttcatgac cacctgtgcc gggattccga gagccagcgc attgcagatg
     5521 aatgtccttt ttcctgcatg ggtactgagc aaagcgtatt tgtgtgtgac ttcatcaata
     5581 cgttcatttc ccttgtagta ggtttcccgt acaggctcgt tgatttctgc cagttcgccc
     5641 agctctttca ggtaatcgtt catcttctgg ttgctgatga cgggcagagc catgtaattc
     5701 tcgaaatgga tgtccttgta tttgtccagt atggctttgc tgtatttgtt cagttcaatc
     5761 gtcaggctgt cggcagtctt gactgtggtt atttcgatgt ggtcggactt cacatcgctt
     5821 cttttcagat tgcgaacatc cgaataccgc aaactcgtaa agcagcagaa caggaaaaca
     5881 tcacgcacac gttccaggta ttgcttatcc ttgggtatct ggtagtcttt cagcttgttc
     5941 agttcatccc aagtcaggaa gattactttt ttcgaggtgg ttttcagttt cggtttgaac
     6001 gtatcgtatg caatgttctg atgatgtcct ttcttgaagc tccagcgcag gaaccatttg
     6061 aggaatccca tttgcttgcc gatggtgctg tttctcatat ccttggtgtc acgcaggaag
     6121 ttgacgtatt cgttcaatcc aaactcgttg aaatagttga acgttgcatc ctccttgaac
     6181 tctttgaggt ggttcctcac tgctgcaaat ttttcatagg tggatgccgt ccagttattc
     6241 tggttaccgc actcttttac aaactcatcg aacacctccc aaaagctgac aggggcttct
     6301 tccggctgtt cttcactggt gtctttcatt ctcatgttga aagcttcctt caactgttgg
     6361 gtcgttggca tgacctcctg cacctcaaat tccttgaaaa tattctggat ttcggcatag
     6421 tatttcagca agtccgtatt gatttcggct gcactttgct ttagcttgtt ggtacatccg
     6481 ttctttaccc gctgcttatc tgcatcccat ttggctacgt caatccggta gcccgttgta
     6541 aactcgatac gttggctggc aaagatgaca cgcatacgga tgggtacgtt ctctacgatt
     6601 ggcacaccgt tctttttccg gctctccaat gcaaaaatga tgttgcgctt gatattcata
     6661 attgggtgcg tttgaaattc tacacccaaa tatacaccca attattgaga tagcaaaaga
     6721 catttagaaa catttacttt tactctatat tgtaatttac acttgattat cagtcgtttg
     6781 cagttttatg atattctgtg aaagtataag ttcgagagcc tgtctctccg caaaaaacgc
     6841 tgaaaatcag cagattgcaa aacaaacacc ctgttttaca cccaagaatg taaagtcggg
     6901 tgtttttgtt ttatttaaga taatacaacc actacataat aaaagagtag cgatattaaa
     6961 agaatccgat gagaaaagac taatatttat ctatccattc agtttgattt ctcaggactt
     7021 tacatcgtcc tgaaagtatt tgttggtacc ggtaccgagg acgcgacagc tgcagacgcg
     7081 ttatgagtaa acttggtctg acagttacca atgcttaatc agtgaggcac ctatctcagc
     7141 gatctgtcta tttcgttcat ccatagttgc ctgactcccc gtcgtgtaga taactacgat
     7201 acgggagggc ttaccatctg gccccagtgc tgcaatgata ccgcgggacc cacgctcacc
     7261 ggctccagat ttatcagcaa taaaccagcc agccggaagg gccgagcgca gaagtggtcc
     7321 tgcaacttta tccgcctcca tccagtctat taattgttgc cgggaagcta gagtaagtag
     7381 ttcgccagtt aatagtttgc gcaacgttgt tgccattgct acaggcatcg tggtgtcacg
     7441 ctcgtcgttt ggtatggctt cattcagctc cggttcccaa cgatcaaggc gagttacatg
     7501 atcccccatg ttgtgcaaaa aagcggttag ctccttcggt cctccgatcg ttgtcagaag
     7561 taagttggcc gcagtgttat cactcatggt tatggcagca ctgcataatt ctcttactgt
     7621 catgccatcc gtaagatgct tttctgtgac tggtgagtac tcaaccaagt cattctgaga
     7681 atagtgtatg cggcgaccga gttgctcttg cccggcgtca atacgggata ataccgcgcc
     7741 acatagcaga actttaaaag tgctcatcat tggaaaacgt tcttcggggc gaaaactctc
     7801 aaggatctta ccgctgttga gatccagttc gatgtaaccc actcgtgcac ccaactgatc
     7861 ttcagcatct tttactttca ccagcgtttc tgggtgagca aaaacaggaa ggcaaaatgc
     7921 cgcaaaaaag ggaataaggg cgacacggaa atgttgaata ctcatactct tcctttttca
     7981 atcatggccg cgggattaaa agtcggggat tggtgaacaa aaaggtgttt ctctctttaa
     8041 gagaaatatc gttttgctaa acagttgata ttgaggtatc attttatcgt aaaagacatt
     8101 tttgctcaac aattgcttga cggaaatcaa caaattttag cattttgtaa aaaagtcgct
     8161 atataatttg gtgaattgga gttattttca tatttttgca tcccgaagag tttctcttaa
     8221 agagagaaac atcttttgca taccttttcc gaccgaattt ttatgtcgta aagaggggct
     8281 ttgcaggggg tggactcaga aagatgagaa tagatgacta ttgtagttga aacacataga
     8341 aagttgctga tatacagacc gatacgcata tcgggatgaa ccatgagtac gttcttttct
     8401 caaaaaacat aaatattcga aaagagatgc aataaattaa ggagaggtta taatgaacaa
     8461 agtaaatata aaagatagtc aaaattttat tacttcaaaa tatcacatag aaaaaataat
     8521 gaattgcata agtttagatg aaaaagataa catctttgaa ataggtgcag ggaaaggtca
     8581 ttttactgct ggattggtaa agagatgtaa ttttgtaacg gcgatagaaa ttgattctaa
     8641 attatgtgag gtaactcgta ataagctctt aaattatcct aactatcaaa tagtaaatga
     8701 tgatatactg aaatttacat ttcctagcca caatccatat aaaatatttg gcaacatacc
     8761 ttacaacata agcacaaata taattcgaaa aattgttttt gaaagttcag ccacaataag
     8821 ttatttaata gtggaatatg gttttgctaa aatgttatta gatacaaaca gatcactagc
     8881 attgctgtta atggcagagg tagatatttc tatattagca aaaattccta ggtattattt
     8941 ccatccaaaa cctaaagtgg atagcacatt aattgtatta aaaagaaagc cagcaaaaat
     9001 ggcatttaaa gagagaaaaa aatatgaaac ttttgtaatg aaatgggtta acaaagagta
     9061 cgaaaaactg tttacaaaaa atcaatttaa taaagcttta aaacatgcga gaatatatga
     9121 tataaacaat attagtttcg aacaatttgt atcgctattt aatagttata aaatatttaa
     9181 cggctaaaaa caataggcca catgcaactg taaatgttta cgcgggtacc gacaccgcgg
     9241 tggaggggaa ttcccatgtc agccgttaag tgttcctgtg tcactcaaaa ttgctttgag
     9301 aggctctaag ggcttctcag tgcgttacat ccctggcttg ttgtccacaa ccgttaaacc
     9361 ttaaaagctt taaaagcctt atatattctt ttttttctta taaaacttaa aaccttagag
     9421 gctatttaag ttgctgattt atattaattt tattgttcaa acatgagagc ttagtacgtg
     9481 aaacatgaga gcttagtacg ttagccatga gagcttagta cgttagccat gagggtttag
     9541 ttcgttaaac atgagagctt agtacgttaa acatgagagc ttagtacgtg aaacatgaga
     9601 gcttagtacg tactatcaac aggttgaact gctgatcttc agatcctcta cgccggacgc
     9661 atcgtggccg gatcaattcc gttttccgct gcataaccct gcttcggggt cattatagcg
     9721 attttttcgg tatatccatc ctttttcgca cgatatacag gattttgcca aagggttcgt
     9781 gtagactttc cttggtgtat ccaacggcgt cagccgggca ggataggtga agtaggccca
     9841 cccgcgagcg ggtgttcctt cttcactgtc ccttattcgc acctggcggt gctcaacggg
     9901 aatcctgctc tgcgaggctg gccggctacc gccggcgtaa cagatgaggg caagcggatg
     9961 gctgatgaaa ccaagccaac caggaagggc agcccaccta tcacggaatt gatccccctc
    10021 gaattggaat tctagcctgg aatagggaag caccacatca cc
//
