LOCUS       sequence-496755-        7910 bp    DNA     circular SYN 09-JAN-2026
DEFINITION  PBT0722 biosensor circuit in pNBU2 integrative Bacteroides plasmid.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7910)
  TITLE     Tropini Lab plasmids
REFERENCE   2  (bases 1 to 7910)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Jan 9, 2026 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pAH110
FEATURES             Location/Qualifiers
     source          1..7910
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             207..893
                     /codon_start=1
                     /product="bright cysteine-free monomeric red fluorescent 
                     protein with fast and complete maturation (Gadella et al., 
                     2023)"
                     /label=mScarlet3
                     /translation="MDSTEAVIKEFMRFKVHMEGSMNGHEFEIEGEGEGRPYEGTQTAK
                     LKVTKGGPLPFSWDILSPQFMYGSRAFIKHPADIPDYWKQSFPEGFKWERVMIFEDGGT
                     VSVTQDTSLEDGTLIYKVKLRGGNFPPDGPVMQKRTMGWEASTERLYPEDVVLKGDIKM
                     ALRLKDGGRYLADFKTTYKAKKPVQMPGAFNIDRKLDITSHNEDYTVVEQYERSVARHS
                     TGGSGGS"
     primer_bind     complement(337..355)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     terminator      919..946
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     CDS             1313..1936
                     /codon_start=1
                     /gene="tetR from transposon Tn10"
                     /product="tetracycline repressor TetR"
                     /label=TetR
                     /note="TetR binds to the tetracycline operator tetO to 
                     inhibit transcription. This inhibition can be relieved by 
                     adding tetracycline or doxycycline."
                     /translation="MSRLDKSKVINSALELLNEVGIEGLTTRKLAQKLGVEQPTLYWHV
                     KNKRALLDALAIEMLDRHHTHFCPLEGESWQDFLRNNAKSFRCALLSHRDGAKVHLGTR
                     PTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEDQEHQVAKEERETPTT
                     DSMPPLLRQAIELFDHQGAEPAFLFGLELIICGLEKQLKCESGS"
     primer_bind     complement(1386..1405)
                     /label=Tet-R
                     /note="Tetracycline resistance gene, reverse primer"
     protein_bind    2101..2119
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     CDS             join(2206..2208,2209..2211,2212..2925)
                     /codon_start=1
                     /product="monomeric GFP derivative that displays 
                     exceptionally high brightness in cells (Campbell et al., 
                     2020)"
                     /label=mGreenLantern
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVRGEGEGDATNGKLTL
                     KFICTTGKLPVPWPTLVTTLGYGVACFARYPDHMKQHDFFKSAMPEGYVQERTISFKDD
                     GTYKTRAEVKFEGDTLVNRIVLKGIDFKEDGNILGHKLEYNFNSHKVYITADKQKNGIK
                     ANFKTRHNVEDGGVQLADHYQQNTPIGDGPVLLPDNHYLSHQSKLSKDPNEKRDHMVLK
                     ERVTAAGITHDMDELYK"
     primer_bind     complement(2251..2272)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     terminator      2946..2973
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     CDS             complement(join(4953..5744,5745..5813))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     5576..5595
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      7105..7493
                     /label=R6K gamma ori
                     /note="gamma replication origin from E. coli plasmid R6K; 
                     requires the R6K initiator protein pi for replication"
     oriT            7664..7773
                     /note="incP origin of transfer"
ORIGIN
        1 caattgggct accttttttt tgttttgttt gcaatggtta atctattgtt aaaatttaaa
       61 gtttcacttg aactttcaaa taatgttctt atatttgcag tgtcgaaaga aacaaagtag
      121 gactgatcac acaacattta aaaaataaca ttatgaaagc actcgaaaag agattcaaag
      181 gtaatattaa caataattta ttttcaatgg atagcaccga ggcagtgatc aaggagttca
      241 tgcggttcaa ggtgcacatg gagggctcca tgaacggcca cgagttcgag atcgagggcg
      301 agggcgaggg ccgcccctac gagggcaccc agaccgccaa gctgaaggtg accaagggtg
      361 gccccctgcc cttctcctgg gacatcctgt cccctcagtt catgtacggc tccagggcct
      421 tcatcaagca ccccgccgac atccccgact actggaagca gtccttcccc gagggcttca
      481 agtgggagcg cgtgatgatc ttcgaggacg gcggcaccgt gtccgtgacc caggacacct
      541 ccctggagga cggcaccctg atctacaagg tgaagctccg cggcggcaac ttccctcctg
      601 acggccccgt aatgcagaag aggacaatgg gctgggaagc atccaccgag cggttgtacc
      661 ccgaggacgt cgtgctgaag ggcgacatta agatggccct gcgcctgaag gacggcggcc
      721 gctacctggc ggacttcaag accacctaca aggccaagaa gcccgtgcag atgcccggcg
      781 ccttcaacat cgaccgcaag ttggacatca cctcccacaa cgaggactac accgtggtgg
      841 aacagtacga acgctccgtg gcccgccact ccaccggcgg ctccggtggc tcctaataaa
      901 tcctagctga ttagctcgag aaggccatcc tgacggatgg cctttttttt gtatggggac
      961 tctgagaacc ctacctttca aaccccgact catggagtgg aaacattaac cgacaaatcc
     1021 tattaatcag attgtttgca caaataaaag tatgtgctct acacaacata gaaatttgac
     1081 tcaggcatcg aactggcaac taaataaaaa tgatataaac acataatata taaaggttaa
     1141 ggtctacata accaagtttt tactacattc tattcctagt ttcagctacc tttcccattc
     1201 taattattgc atatttattt tgaaaatatg caataagttc ttatctttgc acccgaatta
     1261 aagaaccaga agatgaatca aacattacaa ttacataacg cccttatatt tcatgtccag
     1321 attagataaa agtaaagtga ttaacagcgc attagagctg cttaatgagg tcggaatcga
     1381 aggtttaaca acccgtaaac tcgcccagaa gctaggtgta gagcagccta cattgtattg
     1441 gcatgtaaaa aataagcggg ctttgctcga cgccttagcc attgagatgt tagataggca
     1501 ccatactcac ttttgccctt tagaagggga aagctggcaa gattttttac gtaataacgc
     1561 taaaagtttt agatgtgctt tactaagtca tcgcgatgga gcaaaagtac atttaggtac
     1621 acggcctaca gaaaaacagt atgaaactct cgaaaatcaa ttagcctttt tatgccaaca
     1681 aggtttttca ctagagaatg cattatatgc actcagcgct gtggggcatt ttactttagg
     1741 ttgcgtattg gaagatcaag agcatcaagt cgctaaagaa gaaagggaaa cacctactac
     1801 tgatagtatg ccgccattat tacgacaagc tatcgaatta tttgatcacc aaggtgcaga
     1861 gccagccttc ttattcggcc ttgaattgat catatgcgga ttagaaaaac aacttaaatg
     1921 tgaaagtggg tcttaatggc cacatcggtt cctgctagaa ggcccatcct gacggatggc
     1981 cttttggtat tcgagcatta cctcatactt tggatttttg ttttgtttgc aatggttaat
     2041 ctattgttaa aatttaaagt ttcacttgaa ctttcaaata atgttcttat atttgcagtg
     2101 tccctatcag tgatagagag taggatcaca caacatttaa aaaataacat tatgaaagca
     2161 ctcgaaaaga gattcaaagg taatattaac aataatttat tttcaatggt gagcaagggc
     2221 gaggagctgt tcaccggggt ggtgcccatc ctggtcgagc tggacggcga cgtaaacggc
     2281 cacaagttca gcgtccgcgg cgagggcgag ggcgatgcca ccaacggcaa gctgaccctg
     2341 aagttcatct gcaccaccgg caagctgccc gtgccctggc ccaccctcgt gaccacctta
     2401 ggctacggcg tggcctgctt cgcccgctac cccgaccaca tgaagcagca cgacttcttc
     2461 aagtccgcca tgcccgaagg ctacgtccag gagcgcacca tctctttcaa ggacgacggt
     2521 acctacaaga cccgcgccga ggtgaagttc gagggcgaca ccctggtgaa ccgcatcgtg
     2581 ctgaagggca tcgacttcaa ggaggacggc aacatcctgg ggcacaagct ggagtacaac
     2641 ttcaacagcc acaaggtcta tatcacggcc gacaagcaga agaacggcat caaggctaac
     2701 ttcaagaccc gccacaacgt tgaggacggc ggcgtgcagc tcgccgacca ctaccagcag
     2761 aacaccccca tcggcgacgg ccccgtgctg ctgcccgaca accactacct gagccatcag
     2821 tccaagctga gcaaagaccc caacgagaag cgcgatcaca tggtcctgaa ggagagggtg
     2881 accgccgccg ggattacaca tgacatggac gagctgtaca agtaatagcg ttcagctcga
     2941 ctgtgagaag gccatcctga cggatggcct ttttttttcg ttataactag ttgcgttcct
     3001 agccgctact agtgtaattg cctatcttcc agtgatggaa cagcatttgt gcattggctg
     3061 caacaatcag ccttacttgt gcctgttcta tttccgaacc gaccgcttgt atgaatgcat
     3121 caaaattcgt tttctctacg ttggattcct tgttgctcat attgtgatga taatttctac
     3181 aaatatagtc attggtaact atctatgaaa ctgtttgata cttttatagt tgattaaact
     3241 tgttcatggc atttgcctta atatcatccg ctatgtcaat gtagggtttc atagctttgt
     3301 agtcgctgtg tcccgtccat ttcatgacca cctgtgccgg gattccgaga gccagcgcat
     3361 tgcagatgaa tgtccttttt cctgcatggg tactgagcaa agcgtatttg tgtgtgactt
     3421 catcaatacg ttcatttccc ttgtagtagg tttcccgtac aggctcgttg atttctgcca
     3481 gttcgcccag ctctttcagg taatcgttca tcttctggtt gctgatgacg ggcagagcca
     3541 tgtaattctc gaaatggatg tccttgtatt tgtccagtat ggctttgctg tatttgttca
     3601 gttcaatcgt caggctgtcg gcagtcttga ctgtggttat ttcgatgtgg tcggacttca
     3661 catcgcttct tttcagattg cgaacatccg aataccgcaa actcgtaaag cagcagaaca
     3721 ggaaaacatc acgcacacgt tccaggtatt gcttatcctt gggtatctgg tagtctttca
     3781 gcttgttcag ttcatcccaa gtcaggaaga ttactttttt cgaggtggtt ttcagtttcg
     3841 gtttgaacgt atcgtatgca atgttctgat gatgtccttt cttgaagctc cagcgcagga
     3901 accatttgag gaatcccatt tgcttgccga tggtgctgtt tctcatatcc ttggtgtcac
     3961 gcaggaagtt gacgtattcg ttcaatccaa actcgttgaa atagttgaac gttgcatcct
     4021 ccttgaactc tttgaggtgg ttcctcactg ctgcaaattt ttcataggtg gatgccgtcc
     4081 agttattctg gttaccgcac tcttttacaa actcatcgaa cacctcccaa aagctgacag
     4141 gggcttcttc cggctgttct tcactggtgt ctttcattct catgttgaaa gcttccttca
     4201 actgttgggt cgttggcatg acctcctgca cctcaaattc cttgaaaata ttctggattt
     4261 cggcatagta tttcagcaag tccgtattga tttcggctgc actttgcttt agcttgttgg
     4321 tacatccgtt ctttacccgc tgcttatctg catcccattt ggctacgtca atccggtagc
     4381 ccgttgtaaa ctcgatacgt tggctggcaa agatgacacg catacggatg ggtacgttct
     4441 ctacgattgg cacaccgttc tttttccggc tctccaatgc aaaaatgatg ttgcgcttga
     4501 tattcataat tgggtgcgtt tgaaattcta cacccaaata tacacccaat tattgagata
     4561 gcaaaagaca tttagaaaca tttactttta ctctatattg taatttacac ttgattatca
     4621 gtcgtttgca gttttatgat attctgtgaa agtataagtt cgagagcctg tctctccgca
     4681 aaaaacgctg aaaatcagca gattgcaaaa caaacaccct gttttacacc caagaatgta
     4741 aagtcgggtg tttttgtttt atttaagata atacaaccac tacataataa aagagtagcg
     4801 atattaaaag aatccgatga gaaaagacta atatttatct atccattcag tttgatttct
     4861 caggacttta catcgtcctg aaagtatttg ttggtaccgg taccgaggac gcgacagctg
     4921 cagacgcgtt atgagtaaac ttggtctgac agttaccaat gcttaatcag tgaggcacct
     4981 atctcagcga tctgtctatt tcgttcatcc atagttgcct gactccccgt cgtgtagata
     5041 actacgatac gggagggctt accatctggc cccagtgctg caatgatacc gcgggaccca
     5101 cgctcaccgg ctccagattt atcagcaata aaccagccag ccggaagggc cgagcgcaga
     5161 agtggtcctg caactttatc cgcctccatc cagtctatta attgttgccg ggaagctaga
     5221 gtaagtagtt cgccagttaa tagtttgcgc aacgttgttg ccattgctac aggcatcgtg
     5281 gtgtcacgct cgtcgtttgg tatggcttca ttcagctccg gttcccaacg atcaaggcga
     5341 gttacatgat cccccatgtt gtgcaaaaaa gcggttagct ccttcggtcc tccgatcgtt
     5401 gtcagaagta agttggccgc agtgttatca ctcatggtta tggcagcact gcataattct
     5461 cttactgtca tgccatccgt aagatgcttt tctgtgactg gtgagtactc aaccaagtca
     5521 ttctgagaat agtgtatgcg gcgaccgagt tgctcttgcc cggcgtcaat acgggataat
     5581 accgcgccac atagcagaac tttaaaagtg ctcatcattg gaaaacgttc ttcggggcga
     5641 aaactctcaa ggatcttacc gctgttgaga tccagttcga tgtaacccac tcgtgcaccc
     5701 aactgatctt cagcatcttt tactttcacc agcgtttctg ggtgagcaaa aacaggaagg
     5761 caaaatgccg caaaaaaggg aataagggcg acacggaaat gttgaatact catactcttc
     5821 ctttttcaat catggccgcg ggattaaaag tcggggattg gtgaacaaaa aggtgtttct
     5881 ctctttaaga gaaatatcgt tttgctaaac agttgatatt gaggtatcat tttatcgtaa
     5941 aagacatttt tgctcaacaa ttgcttgacg gaaatcaaca aattttagca ttttgtaaaa
     6001 aagtcgctat ataatttggt gaattggagt tattttcata tttttgcatc ccgaagagtt
     6061 tctcttaaag agagaaacat cttttgcata ccttttccga ccgaattttt atgtcgtaaa
     6121 gaggggcttt gcagggggtg gactcagaaa gatgagaata gatgactatt gtagttgaaa
     6181 cacatagaaa gttgctgata tacagaccga tacgcatatc gggatgaacc atgagtacgt
     6241 tcttttctca aaaaacataa atattcgaaa agagatgcaa taaattaagg agaggttata
     6301 atgaacaaag taaatataaa agatagtcaa aattttatta cttcaaaata tcacatagaa
     6361 aaaataatga attgcataag tttagatgaa aaagataaca tctttgaaat aggtgcaggg
     6421 aaaggtcatt ttactgctgg attggtaaag agatgtaatt ttgtaacggc gatagaaatt
     6481 gattctaaat tatgtgaggt aactcgtaat aagctcttaa attatcctaa ctatcaaata
     6541 gtaaatgatg atatactgaa atttacattt cctagccaca atccatataa aatatttggc
     6601 aacatacctt acaacataag cacaaatata attcgaaaaa ttgtttttga aagttcagcc
     6661 acaataagtt atttaatagt ggaatatggt tttgctaaaa tgttattaga tacaaacaga
     6721 tcactagcat tgctgttaat ggcagaggta gatatttcta tattagcaaa aattcctagg
     6781 tattatttcc atccaaaacc taaagtggat agcacattaa ttgtattaaa aagaaagcca
     6841 gcaaaaatgg catttaaaga gagaaaaaaa tatgaaactt ttgtaatgaa atgggttaac
     6901 aaagagtacg aaaaactgtt tacaaaaaat caatttaata aagctttaaa acatgcgaga
     6961 atatatgata taaacaatat tagtttcgaa caatttgtat cgctatttaa tagttataaa
     7021 atatttaacg gctaaaaaca ataggccaca tgcaactgta aatgtttacg cgggtaccga
     7081 caccgcggtg gaggggaatt cccatgtcag ccgttaagtg ttcctgtgtc actcaaaatt
     7141 gctttgagag gctctaaggg cttctcagtg cgttacatcc ctggcttgtt gtccacaacc
     7201 gttaaacctt aaaagcttta aaagccttat atattctttt ttttcttata aaacttaaaa
     7261 ccttagaggc tatttaagtt gctgatttat attaatttta ttgttcaaac atgagagctt
     7321 agtacgtgaa acatgagagc ttagtacgtt agccatgaga gcttagtacg ttagccatga
     7381 gggtttagtt cgttaaacat gagagcttag tacgttaaac atgagagctt agtacgtgaa
     7441 acatgagagc ttagtacgta ctatcaacag gttgaactgc tgatcttcag atcctctacg
     7501 ccggacgcat cgtggccgga tcaattccgt tttccgctgc ataaccctgc ttcggggtca
     7561 ttatagcgat tttttcggta tatccatcct ttttcgcacg atatacagga ttttgccaaa
     7621 gggttcgtgt agactttcct tggtgtatcc aacggcgtca gccgggcagg ataggtgaag
     7681 taggcccacc cgcgagcggg tgttccttct tcactgtccc ttattcgcac ctggcggtgc
     7741 tcaacgggaa tcctgctctg cgaggctggc cggctaccgc cggcgtaaca gatgagggca
     7801 agcggatggc tgatgaaacc aagccaacca ggaagggcag cccacctatc acggaattga
     7861 tccccctcga attggaattc tagcctggaa tagggaagca ccacatcacc
//
