LOCUS       sequence-496756-        8045 bp    DNA     circular SYN 09-JAN-2026
DEFINITION  PBT1004 biosensor circuit in pNBU2 integrative Bacteroides plasmid.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8045)
  TITLE     Tropini Lab plasmids
REFERENCE   2  (bases 1 to 8045)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Jan 9, 2026 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pAH120
FEATURES             Location/Qualifiers
     source          1..8045
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             207..893
                     /codon_start=1
                     /product="bright cysteine-free monomeric red fluorescent 
                     protein with fast and complete maturation (Gadella et al., 
                     2023)"
                     /label=mScarlet3
                     /translation="MDSTEAVIKEFMRFKVHMEGSMNGHEFEIEGEGEGRPYEGTQTAK
                     LKVTKGGPLPFSWDILSPQFMYGSRAFIKHPADIPDYWKQSFPEGFKWERVMIFEDGGT
                     VSVTQDTSLEDGTLIYKVKLRGGNFPPDGPVMQKRTMGWEASTERLYPEDVVLKGDIKM
                     ALRLKDGGRYLADFKTTYKAKKPVQMPGAFNIDRKLDITSHNEDYTVVEQYERSVARHS
                     TGGSGGS"
     primer_bind     complement(337..355)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     terminator      919..946
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     CDS             1448..2071
                     /codon_start=1
                     /gene="tetR from transposon Tn10"
                     /product="tetracycline repressor TetR"
                     /label=TetR
                     /note="TetR binds to the tetracycline operator tetO to 
                     inhibit transcription. This inhibition can be relieved by 
                     adding tetracycline or doxycycline."
                     /translation="MSRLDKSKVINSALELLNEVGIEGLTTRKLAQKLGVEQPTLYWHV
                     KNKRALLDALAIEMLDRHHTHFCPLEGESWQDFLRNNAKSFRCALLSHRDGAKVHLGTR
                     PTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEDQEHQVAKEERETPTT
                     DSMPPLLRQAIELFDHQGAEPAFLFGLELIICGLEKQLKCESGS"
     primer_bind     complement(1521..1540)
                     /label=Tet-R
                     /note="Tetracycline resistance gene, reverse primer"
     protein_bind    2236..2254
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     CDS             join(2341..2343,2344..2346,2347..3060)
                     /codon_start=1
                     /product="monomeric GFP derivative that displays 
                     exceptionally high brightness in cells (Campbell et al., 
                     2020)"
                     /label=mGreenLantern
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVRGEGEGDATNGKLTL
                     KFICTTGKLPVPWPTLVTTLGYGVACFARYPDHMKQHDFFKSAMPEGYVQERTISFKDD
                     GTYKTRAEVKFEGDTLVNRIVLKGIDFKEDGNILGHKLEYNFNSHKVYITADKQKNGIK
                     ANFKTRHNVEDGGVQLADHYQQNTPIGDGPVLLPDNHYLSHQSKLSKDPNEKRDHMVLK
                     ERVTAAGITHDMDELYK"
     primer_bind     complement(2386..2407)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     terminator      3081..3108
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     CDS             complement(join(5088..5879,5880..5948))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     5711..5730
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      7240..7628
                     /label=R6K gamma ori
                     /note="gamma replication origin from E. coli plasmid R6K; 
                     requires the R6K initiator protein pi for replication"
     oriT            7799..7908
                     /note="incP origin of transfer"
ORIGIN
        1 caattgggct accttttttt tgttttgttt gcaatggtta atctattgtt aaaatttaaa
       61 gtttcacttg aactttcaaa taatgttctt atatttgcag tgtcgaaaga aacaaagtag
      121 gactgatcac acaacattta aaaaataaca ttatgaaagc actcgaaaag agattcaaag
      181 gtaatattaa caataattta ttttcaatgg atagcaccga ggcagtgatc aaggagttca
      241 tgcggttcaa ggtgcacatg gagggctcca tgaacggcca cgagttcgag atcgagggcg
      301 agggcgaggg ccgcccctac gagggcaccc agaccgccaa gctgaaggtg accaagggtg
      361 gccccctgcc cttctcctgg gacatcctgt cccctcagtt catgtacggc tccagggcct
      421 tcatcaagca ccccgccgac atccccgact actggaagca gtccttcccc gagggcttca
      481 agtgggagcg cgtgatgatc ttcgaggacg gcggcaccgt gtccgtgacc caggacacct
      541 ccctggagga cggcaccctg atctacaagg tgaagctccg cggcggcaac ttccctcctg
      601 acggccccgt aatgcagaag aggacaatgg gctgggaagc atccaccgag cggttgtacc
      661 ccgaggacgt cgtgctgaag ggcgacatta agatggccct gcgcctgaag gacggcggcc
      721 gctacctggc ggacttcaag accacctaca aggccaagaa gcccgtgcag atgcccggcg
      781 ccttcaacat cgaccgcaag ttggacatca cctcccacaa cgaggactac accgtggtgg
      841 aacagtacga acgctccgtg gcccgccact ccaccggcgg ctccggtggc tcctaataaa
      901 tcctagctga ttagctcgag aaggccatcc tgacggatgg cctttttttt gtatggggac
      961 tctgagaacc ctacctttca aaccccgatc agatgtgcgg agatatgctt ggcatatctc
     1021 cgttttaatt gacataatcc gccggagtaa agggatgaat gatgcttacc gacgtgattt
     1081 aatcgttttt taataaatga aatttatttt ttcctgatta tacaaatagt acaaaagaat
     1141 cttcttgtct ggtcttgttt tttatattcc gcaacatttc atcctttgtg tcatagtatt
     1201 agtcaccttc gaaacattta ttgtttgtat ccgacatgga tggagtttgg ctagtcccca
     1261 aatatgcgga tatttgtgaa gtgagatttc actatcaaaa gtattggtcc tttcggaccg
     1321 gccgcctgtg aaggtagctc tattgtttgt tttgtttgtg tttggtgcat cttttttcaa
     1381 gcgagaaaga agatgcacta tttatatcta tctaccctga ctatcggata ttgaatgaaa
     1441 tttgcctatg tccagattag ataaaagtaa agtgattaac agcgcattag agctgcttaa
     1501 tgaggtcgga atcgaaggtt taacaacccg taaactcgcc cagaagctag gtgtagagca
     1561 gcctacattg tattggcatg taaaaaataa gcgggctttg ctcgacgcct tagccattga
     1621 gatgttagat aggcaccata ctcacttttg ccctttagaa ggggaaagct ggcaagattt
     1681 tttacgtaat aacgctaaaa gttttagatg tgctttacta agtcatcgcg atggagcaaa
     1741 agtacattta ggtacacggc ctacagaaaa acagtatgaa actctcgaaa atcaattagc
     1801 ctttttatgc caacaaggtt tttcactaga gaatgcatta tatgcactca gcgctgtggg
     1861 gcattttact ttaggttgcg tattggaaga tcaagagcat caagtcgcta aagaagaaag
     1921 ggaaacacct actactgata gtatgccgcc attattacga caagctatcg aattatttga
     1981 tcaccaaggt gcagagccag ccttcttatt cggccttgaa ttgatcatat gcggattaga
     2041 aaaacaactt aaatgtgaaa gtgggtctta atggccacat cggttcctgc tagaaggccc
     2101 atcctgacgg atggcctttt ggtattcgag cattacctca tactttggat ttttgttttg
     2161 tttgcaatgg ttaatctatt gttaaaattt aaagtttcac ttgaactttc aaataatgtt
     2221 cttatatttg cagtgtccct atcagtgata gagagtagga tcacacaaca tttaaaaaat
     2281 aacattatga aagcactcga aaagagattc aaaggtaata ttaacaataa tttattttca
     2341 atggtgagca agggcgagga gctgttcacc ggggtggtgc ccatcctggt cgagctggac
     2401 ggcgacgtaa acggccacaa gttcagcgtc cgcggcgagg gcgagggcga tgccaccaac
     2461 ggcaagctga ccctgaagtt catctgcacc accggcaagc tgcccgtgcc ctggcccacc
     2521 ctcgtgacca ccttaggcta cggcgtggcc tgcttcgccc gctaccccga ccacatgaag
     2581 cagcacgact tcttcaagtc cgccatgccc gaaggctacg tccaggagcg caccatctct
     2641 ttcaaggacg acggtaccta caagacccgc gccgaggtga agttcgaggg cgacaccctg
     2701 gtgaaccgca tcgtgctgaa gggcatcgac ttcaaggagg acggcaacat cctggggcac
     2761 aagctggagt acaacttcaa cagccacaag gtctatatca cggccgacaa gcagaagaac
     2821 ggcatcaagg ctaacttcaa gacccgccac aacgttgagg acggcggcgt gcagctcgcc
     2881 gaccactacc agcagaacac ccccatcggc gacggccccg tgctgctgcc cgacaaccac
     2941 tacctgagcc atcagtccaa gctgagcaaa gaccccaacg agaagcgcga tcacatggtc
     3001 ctgaaggaga gggtgaccgc cgccgggatt acacatgaca tggacgagct gtacaagtaa
     3061 tagcgttcag ctcgactgtg agaaggccat cctgacggat ggcctttttt tttcgttata
     3121 actagttgcg ttcctagccg ctactagtgt aattgcctat cttccagtga tggaacagca
     3181 tttgtgcatt ggctgcaaca atcagcctta cttgtgcctg ttctatttcc gaaccgaccg
     3241 cttgtatgaa tgcatcaaaa ttcgttttct ctacgttgga ttccttgttg ctcatattgt
     3301 gatgataatt tctacaaata tagtcattgg taactatcta tgaaactgtt tgatactttt
     3361 atagttgatt aaacttgttc atggcatttg ccttaatatc atccgctatg tcaatgtagg
     3421 gtttcatagc tttgtagtcg ctgtgtcccg tccatttcat gaccacctgt gccgggattc
     3481 cgagagccag cgcattgcag atgaatgtcc tttttcctgc atgggtactg agcaaagcgt
     3541 atttgtgtgt gacttcatca atacgttcat ttcccttgta gtaggtttcc cgtacaggct
     3601 cgttgatttc tgccagttcg cccagctctt tcaggtaatc gttcatcttc tggttgctga
     3661 tgacgggcag agccatgtaa ttctcgaaat ggatgtcctt gtatttgtcc agtatggctt
     3721 tgctgtattt gttcagttca atcgtcaggc tgtcggcagt cttgactgtg gttatttcga
     3781 tgtggtcgga cttcacatcg cttcttttca gattgcgaac atccgaatac cgcaaactcg
     3841 taaagcagca gaacaggaaa acatcacgca cacgttccag gtattgctta tccttgggta
     3901 tctggtagtc tttcagcttg ttcagttcat cccaagtcag gaagattact tttttcgagg
     3961 tggttttcag tttcggtttg aacgtatcgt atgcaatgtt ctgatgatgt cctttcttga
     4021 agctccagcg caggaaccat ttgaggaatc ccatttgctt gccgatggtg ctgtttctca
     4081 tatccttggt gtcacgcagg aagttgacgt attcgttcaa tccaaactcg ttgaaatagt
     4141 tgaacgttgc atcctccttg aactctttga ggtggttcct cactgctgca aatttttcat
     4201 aggtggatgc cgtccagtta ttctggttac cgcactcttt tacaaactca tcgaacacct
     4261 cccaaaagct gacaggggct tcttccggct gttcttcact ggtgtctttc attctcatgt
     4321 tgaaagcttc cttcaactgt tgggtcgttg gcatgacctc ctgcacctca aattccttga
     4381 aaatattctg gatttcggca tagtatttca gcaagtccgt attgatttcg gctgcacttt
     4441 gctttagctt gttggtacat ccgttcttta cccgctgctt atctgcatcc catttggcta
     4501 cgtcaatccg gtagcccgtt gtaaactcga tacgttggct ggcaaagatg acacgcatac
     4561 ggatgggtac gttctctacg attggcacac cgttcttttt ccggctctcc aatgcaaaaa
     4621 tgatgttgcg cttgatattc ataattgggt gcgtttgaaa ttctacaccc aaatatacac
     4681 ccaattattg agatagcaaa agacatttag aaacatttac ttttactcta tattgtaatt
     4741 tacacttgat tatcagtcgt ttgcagtttt atgatattct gtgaaagtat aagttcgaga
     4801 gcctgtctct ccgcaaaaaa cgctgaaaat cagcagattg caaaacaaac accctgtttt
     4861 acacccaaga atgtaaagtc gggtgttttt gttttattta agataataca accactacat
     4921 aataaaagag tagcgatatt aaaagaatcc gatgagaaaa gactaatatt tatctatcca
     4981 ttcagtttga tttctcagga ctttacatcg tcctgaaagt atttgttggt accggtaccg
     5041 aggacgcgac agctgcagac gcgttatgag taaacttggt ctgacagtta ccaatgctta
     5101 atcagtgagg cacctatctc agcgatctgt ctatttcgtt catccatagt tgcctgactc
     5161 cccgtcgtgt agataactac gatacgggag ggcttaccat ctggccccag tgctgcaatg
     5221 ataccgcggg acccacgctc accggctcca gatttatcag caataaacca gccagccgga
     5281 agggccgagc gcagaagtgg tcctgcaact ttatccgcct ccatccagtc tattaattgt
     5341 tgccgggaag ctagagtaag tagttcgcca gttaatagtt tgcgcaacgt tgttgccatt
     5401 gctacaggca tcgtggtgtc acgctcgtcg tttggtatgg cttcattcag ctccggttcc
     5461 caacgatcaa ggcgagttac atgatccccc atgttgtgca aaaaagcggt tagctccttc
     5521 ggtcctccga tcgttgtcag aagtaagttg gccgcagtgt tatcactcat ggttatggca
     5581 gcactgcata attctcttac tgtcatgcca tccgtaagat gcttttctgt gactggtgag
     5641 tactcaacca agtcattctg agaatagtgt atgcggcgac cgagttgctc ttgcccggcg
     5701 tcaatacggg ataataccgc gccacatagc agaactttaa aagtgctcat cattggaaaa
     5761 cgttcttcgg ggcgaaaact ctcaaggatc ttaccgctgt tgagatccag ttcgatgtaa
     5821 cccactcgtg cacccaactg atcttcagca tcttttactt tcaccagcgt ttctgggtga
     5881 gcaaaaacag gaaggcaaaa tgccgcaaaa aagggaataa gggcgacacg gaaatgttga
     5941 atactcatac tcttcctttt tcaatcatgg ccgcgggatt aaaagtcggg gattggtgaa
     6001 caaaaaggtg tttctctctt taagagaaat atcgttttgc taaacagttg atattgaggt
     6061 atcattttat cgtaaaagac atttttgctc aacaattgct tgacggaaat caacaaattt
     6121 tagcattttg taaaaaagtc gctatataat ttggtgaatt ggagttattt tcatattttt
     6181 gcatcccgaa gagtttctct taaagagaga aacatctttt gcataccttt tccgaccgaa
     6241 tttttatgtc gtaaagaggg gctttgcagg gggtggactc agaaagatga gaatagatga
     6301 ctattgtagt tgaaacacat agaaagttgc tgatatacag accgatacgc atatcgggat
     6361 gaaccatgag tacgttcttt tctcaaaaaa cataaatatt cgaaaagaga tgcaataaat
     6421 taaggagagg ttataatgaa caaagtaaat ataaaagata gtcaaaattt tattacttca
     6481 aaatatcaca tagaaaaaat aatgaattgc ataagtttag atgaaaaaga taacatcttt
     6541 gaaataggtg cagggaaagg tcattttact gctggattgg taaagagatg taattttgta
     6601 acggcgatag aaattgattc taaattatgt gaggtaactc gtaataagct cttaaattat
     6661 cctaactatc aaatagtaaa tgatgatata ctgaaattta catttcctag ccacaatcca
     6721 tataaaatat ttggcaacat accttacaac ataagcacaa atataattcg aaaaattgtt
     6781 tttgaaagtt cagccacaat aagttattta atagtggaat atggttttgc taaaatgtta
     6841 ttagatacaa acagatcact agcattgctg ttaatggcag aggtagatat ttctatatta
     6901 gcaaaaattc ctaggtatta tttccatcca aaacctaaag tggatagcac attaattgta
     6961 ttaaaaagaa agccagcaaa aatggcattt aaagagagaa aaaaatatga aacttttgta
     7021 atgaaatggg ttaacaaaga gtacgaaaaa ctgtttacaa aaaatcaatt taataaagct
     7081 ttaaaacatg cgagaatata tgatataaac aatattagtt tcgaacaatt tgtatcgcta
     7141 tttaatagtt ataaaatatt taacggctaa aaacaatagg ccacatgcaa ctgtaaatgt
     7201 ttacgcgggt accgacaccg cggtggaggg gaattcccat gtcagccgtt aagtgttcct
     7261 gtgtcactca aaattgcttt gagaggctct aagggcttct cagtgcgtta catccctggc
     7321 ttgttgtcca caaccgttaa accttaaaag ctttaaaagc cttatatatt cttttttttc
     7381 ttataaaact taaaacctta gaggctattt aagttgctga tttatattaa ttttattgtt
     7441 caaacatgag agcttagtac gtgaaacatg agagcttagt acgttagcca tgagagctta
     7501 gtacgttagc catgagggtt tagttcgtta aacatgagag cttagtacgt taaacatgag
     7561 agcttagtac gtgaaacatg agagcttagt acgtactatc aacaggttga actgctgatc
     7621 ttcagatcct ctacgccgga cgcatcgtgg ccggatcaat tccgttttcc gctgcataac
     7681 cctgcttcgg ggtcattata gcgatttttt cggtatatcc atcctttttc gcacgatata
     7741 caggattttg ccaaagggtt cgtgtagact ttccttggtg tatccaacgg cgtcagccgg
     7801 gcaggatagg tgaagtaggc ccacccgcga gcgggtgttc cttcttcact gtcccttatt
     7861 cgcacctggc ggtgctcaac gggaatcctg ctctgcgagg ctggccggct accgccggcg
     7921 taacagatga gggcaagcgg atggctgatg aaaccaagcc aaccaggaag ggcagcccac
     7981 ctatcacgga attgatcccc ctcgaattgg aattctagcc tggaataggg aagcaccaca
     8041 tcacc
//
