LOCUS       sequence-496757-        8058 bp    DNA     circular SYN 09-JAN-2026
DEFINITION  PBT0733 biosensor circuit in pNBU2 integrative Bacteroides plasmid.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8058)
  TITLE     Tropini Lab plasmids
REFERENCE   2  (bases 1 to 8058)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Jan 9, 2026 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pAH111
FEATURES             Location/Qualifiers
     source          1..8058
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             207..893
                     /codon_start=1
                     /product="bright cysteine-free monomeric red fluorescent 
                     protein with fast and complete maturation (Gadella et al., 
                     2023)"
                     /label=mScarlet3
                     /translation="MDSTEAVIKEFMRFKVHMEGSMNGHEFEIEGEGEGRPYEGTQTAK
                     LKVTKGGPLPFSWDILSPQFMYGSRAFIKHPADIPDYWKQSFPEGFKWERVMIFEDGGT
                     VSVTQDTSLEDGTLIYKVKLRGGNFPPDGPVMQKRTMGWEASTERLYPEDVVLKGDIKM
                     ALRLKDGGRYLADFKTTYKAKKPVQMPGAFNIDRKLDITSHNEDYTVVEQYERSVARHS
                     TGGSGGS"
     primer_bind     complement(337..355)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     terminator      919..946
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     CDS             1461..2084
                     /codon_start=1
                     /gene="tetR from transposon Tn10"
                     /product="tetracycline repressor TetR"
                     /label=TetR
                     /note="TetR binds to the tetracycline operator tetO to 
                     inhibit transcription. This inhibition can be relieved by 
                     adding tetracycline or doxycycline."
                     /translation="MSRLDKSKVINSALELLNEVGIEGLTTRKLAQKLGVEQPTLYWHV
                     KNKRALLDALAIEMLDRHHTHFCPLEGESWQDFLRNNAKSFRCALLSHRDGAKVHLGTR
                     PTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEDQEHQVAKEERETPTT
                     DSMPPLLRQAIELFDHQGAEPAFLFGLELIICGLEKQLKCESGS"
     primer_bind     complement(1534..1553)
                     /label=Tet-R
                     /note="Tetracycline resistance gene, reverse primer"
     protein_bind    2249..2267
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     CDS             join(2354..2356,2357..2359,2360..3073)
                     /codon_start=1
                     /product="monomeric GFP derivative that displays 
                     exceptionally high brightness in cells (Campbell et al., 
                     2020)"
                     /label=mGreenLantern
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVRGEGEGDATNGKLTL
                     KFICTTGKLPVPWPTLVTTLGYGVACFARYPDHMKQHDFFKSAMPEGYVQERTISFKDD
                     GTYKTRAEVKFEGDTLVNRIVLKGIDFKEDGNILGHKLEYNFNSHKVYITADKQKNGIK
                     ANFKTRHNVEDGGVQLADHYQQNTPIGDGPVLLPDNHYLSHQSKLSKDPNEKRDHMVLK
                     ERVTAAGITHDMDELYK"
     primer_bind     complement(2399..2420)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     terminator      3094..3121
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     CDS             complement(join(5101..5892,5893..5961))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     5724..5743
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      7253..7641
                     /label=R6K gamma ori
                     /note="gamma replication origin from E. coli plasmid R6K; 
                     requires the R6K initiator protein pi for replication"
     oriT            7812..7921
                     /note="incP origin of transfer"
ORIGIN
        1 caattgggct accttttttt tgttttgttt gcaatggtta atctattgtt aaaatttaaa
       61 gtttcacttg aactttcaaa taatgttctt atatttgcag tgtcgaaaga aacaaagtag
      121 gactgatcac acaacattta aaaaataaca ttatgaaagc actcgaaaag agattcaaag
      181 gtaatattaa caataattta ttttcaatgg atagcaccga ggcagtgatc aaggagttca
      241 tgcggttcaa ggtgcacatg gagggctcca tgaacggcca cgagttcgag atcgagggcg
      301 agggcgaggg ccgcccctac gagggcaccc agaccgccaa gctgaaggtg accaagggtg
      361 gccccctgcc cttctcctgg gacatcctgt cccctcagtt catgtacggc tccagggcct
      421 tcatcaagca ccccgccgac atccccgact actggaagca gtccttcccc gagggcttca
      481 agtgggagcg cgtgatgatc ttcgaggacg gcggcaccgt gtccgtgacc caggacacct
      541 ccctggagga cggcaccctg atctacaagg tgaagctccg cggcggcaac ttccctcctg
      601 acggccccgt aatgcagaag aggacaatgg gctgggaagc atccaccgag cggttgtacc
      661 ccgaggacgt cgtgctgaag ggcgacatta agatggccct gcgcctgaag gacggcggcc
      721 gctacctggc ggacttcaag accacctaca aggccaagaa gcccgtgcag atgcccggcg
      781 ccttcaacat cgaccgcaag ttggacatca cctcccacaa cgaggactac accgtggtgg
      841 aacagtacga acgctccgtg gcccgccact ccaccggcgg ctccggtggc tcctaataaa
      901 tcctagctga ttagctcgag aaggccatcc tgacggatgg cctttttttt gtatggggac
      961 tctgagaacc ctacctttca aaccccgagt cgtataccag tttcccctgt tcggctttta
     1021 ctaccggacg ttcgccggtg atcatgactt cgggaagttc ccgatacagg ctgtctatat
     1081 aggctacgtt ttttacgctg tcggcaggtt cctgcgctgt ctgtacctgc tgtgcagaag
     1141 cgtacatcca atacatgaaa agtaacaaaa gcagtaaata agttcgtttc atatcgcatt
     1201 tatttttgag tatgctgcaa aatacgggag aaaaaacgaa actttacgtt atcgccggct
     1261 tatctagtgt tatttagtct tatcaaagag aagaaaaata acagtttcat actatctttg
     1321 tccgtatgaa actaccgttg aaacatatca ttatccttgt catctgctcg ctgacaggca
     1381 tctttgttta ccagacatat tggctgacag ggctttaccg cacgatgaag caggaaatga
     1441 acaataatat caaagacgcc atgtccagat tagataaaag taaagtgatt aacagcgcat
     1501 tagagctgct taatgaggtc ggaatcgaag gtttaacaac ccgtaaactc gcccagaagc
     1561 taggtgtaga gcagcctaca ttgtattggc atgtaaaaaa taagcgggct ttgctcgacg
     1621 ccttagccat tgagatgtta gataggcacc atactcactt ttgcccttta gaaggggaaa
     1681 gctggcaaga ttttttacgt aataacgcta aaagttttag atgtgcttta ctaagtcatc
     1741 gcgatggagc aaaagtacat ttaggtacac ggcctacaga aaaacagtat gaaactctcg
     1801 aaaatcaatt agccttttta tgccaacaag gtttttcact agagaatgca ttatatgcac
     1861 tcagcgctgt ggggcatttt actttaggtt gcgtattgga agatcaagag catcaagtcg
     1921 ctaaagaaga aagggaaaca cctactactg atagtatgcc gccattatta cgacaagcta
     1981 tcgaattatt tgatcaccaa ggtgcagagc cagccttctt attcggcctt gaattgatca
     2041 tatgcggatt agaaaaacaa cttaaatgtg aaagtgggtc ttaatggcca catcggttcc
     2101 tgctagaagg cccatcctga cggatggcct tttggtattc gagcattacc tcatactttg
     2161 gatttttgtt ttgtttgcaa tggttaatct attgttaaaa tttaaagttt cacttgaact
     2221 ttcaaataat gttcttatat ttgcagtgtc cctatcagtg atagagagta ggatcacaca
     2281 acatttaaaa aataacatta tgaaagcact cgaaaagaga ttcaaaggta atattaacaa
     2341 taatttattt tcaatggtga gcaagggcga ggagctgttc accggggtgg tgcccatcct
     2401 ggtcgagctg gacggcgacg taaacggcca caagttcagc gtccgcggcg agggcgaggg
     2461 cgatgccacc aacggcaagc tgaccctgaa gttcatctgc accaccggca agctgcccgt
     2521 gccctggccc accctcgtga ccaccttagg ctacggcgtg gcctgcttcg cccgctaccc
     2581 cgaccacatg aagcagcacg acttcttcaa gtccgccatg cccgaaggct acgtccagga
     2641 gcgcaccatc tctttcaagg acgacggtac ctacaagacc cgcgccgagg tgaagttcga
     2701 gggcgacacc ctggtgaacc gcatcgtgct gaagggcatc gacttcaagg aggacggcaa
     2761 catcctgggg cacaagctgg agtacaactt caacagccac aaggtctata tcacggccga
     2821 caagcagaag aacggcatca aggctaactt caagacccgc cacaacgttg aggacggcgg
     2881 cgtgcagctc gccgaccact accagcagaa cacccccatc ggcgacggcc ccgtgctgct
     2941 gcccgacaac cactacctga gccatcagtc caagctgagc aaagacccca acgagaagcg
     3001 cgatcacatg gtcctgaagg agagggtgac cgccgccggg attacacatg acatggacga
     3061 gctgtacaag taatagcgtt cagctcgact gtgagaaggc catcctgacg gatggccttt
     3121 ttttttcgtt ataactagtt gcgttcctag ccgctactag tgtaattgcc tatcttccag
     3181 tgatggaaca gcatttgtgc attggctgca acaatcagcc ttacttgtgc ctgttctatt
     3241 tccgaaccga ccgcttgtat gaatgcatca aaattcgttt tctctacgtt ggattccttg
     3301 ttgctcatat tgtgatgata atttctacaa atatagtcat tggtaactat ctatgaaact
     3361 gtttgatact tttatagttg attaaacttg ttcatggcat ttgccttaat atcatccgct
     3421 atgtcaatgt agggtttcat agctttgtag tcgctgtgtc ccgtccattt catgaccacc
     3481 tgtgccggga ttccgagagc cagcgcattg cagatgaatg tcctttttcc tgcatgggta
     3541 ctgagcaaag cgtatttgtg tgtgacttca tcaatacgtt catttccctt gtagtaggtt
     3601 tcccgtacag gctcgttgat ttctgccagt tcgcccagct ctttcaggta atcgttcatc
     3661 ttctggttgc tgatgacggg cagagccatg taattctcga aatggatgtc cttgtatttg
     3721 tccagtatgg ctttgctgta tttgttcagt tcaatcgtca ggctgtcggc agtcttgact
     3781 gtggttattt cgatgtggtc ggacttcaca tcgcttcttt tcagattgcg aacatccgaa
     3841 taccgcaaac tcgtaaagca gcagaacagg aaaacatcac gcacacgttc caggtattgc
     3901 ttatccttgg gtatctggta gtctttcagc ttgttcagtt catcccaagt caggaagatt
     3961 acttttttcg aggtggtttt cagtttcggt ttgaacgtat cgtatgcaat gttctgatga
     4021 tgtcctttct tgaagctcca gcgcaggaac catttgagga atcccatttg cttgccgatg
     4081 gtgctgtttc tcatatcctt ggtgtcacgc aggaagttga cgtattcgtt caatccaaac
     4141 tcgttgaaat agttgaacgt tgcatcctcc ttgaactctt tgaggtggtt cctcactgct
     4201 gcaaattttt cataggtgga tgccgtccag ttattctggt taccgcactc ttttacaaac
     4261 tcatcgaaca cctcccaaaa gctgacaggg gcttcttccg gctgttcttc actggtgtct
     4321 ttcattctca tgttgaaagc ttccttcaac tgttgggtcg ttggcatgac ctcctgcacc
     4381 tcaaattcct tgaaaatatt ctggatttcg gcatagtatt tcagcaagtc cgtattgatt
     4441 tcggctgcac tttgctttag cttgttggta catccgttct ttacccgctg cttatctgca
     4501 tcccatttgg ctacgtcaat ccggtagccc gttgtaaact cgatacgttg gctggcaaag
     4561 atgacacgca tacggatggg tacgttctct acgattggca caccgttctt tttccggctc
     4621 tccaatgcaa aaatgatgtt gcgcttgata ttcataattg ggtgcgtttg aaattctaca
     4681 cccaaatata cacccaatta ttgagatagc aaaagacatt tagaaacatt tacttttact
     4741 ctatattgta atttacactt gattatcagt cgtttgcagt tttatgatat tctgtgaaag
     4801 tataagttcg agagcctgtc tctccgcaaa aaacgctgaa aatcagcaga ttgcaaaaca
     4861 aacaccctgt tttacaccca agaatgtaaa gtcgggtgtt tttgttttat ttaagataat
     4921 acaaccacta cataataaaa gagtagcgat attaaaagaa tccgatgaga aaagactaat
     4981 atttatctat ccattcagtt tgatttctca ggactttaca tcgtcctgaa agtatttgtt
     5041 ggtaccggta ccgaggacgc gacagctgca gacgcgttat gagtaaactt ggtctgacag
     5101 ttaccaatgc ttaatcagtg aggcacctat ctcagcgatc tgtctatttc gttcatccat
     5161 agttgcctga ctccccgtcg tgtagataac tacgatacgg gagggcttac catctggccc
     5221 cagtgctgca atgataccgc gggacccacg ctcaccggct ccagatttat cagcaataaa
     5281 ccagccagcc ggaagggccg agcgcagaag tggtcctgca actttatccg cctccatcca
     5341 gtctattaat tgttgccggg aagctagagt aagtagttcg ccagttaata gtttgcgcaa
     5401 cgttgttgcc attgctacag gcatcgtggt gtcacgctcg tcgtttggta tggcttcatt
     5461 cagctccggt tcccaacgat caaggcgagt tacatgatcc cccatgttgt gcaaaaaagc
     5521 ggttagctcc ttcggtcctc cgatcgttgt cagaagtaag ttggccgcag tgttatcact
     5581 catggttatg gcagcactgc ataattctct tactgtcatg ccatccgtaa gatgcttttc
     5641 tgtgactggt gagtactcaa ccaagtcatt ctgagaatag tgtatgcggc gaccgagttg
     5701 ctcttgcccg gcgtcaatac gggataatac cgcgccacat agcagaactt taaaagtgct
     5761 catcattgga aaacgttctt cggggcgaaa actctcaagg atcttaccgc tgttgagatc
     5821 cagttcgatg taacccactc gtgcacccaa ctgatcttca gcatctttta ctttcaccag
     5881 cgtttctggg tgagcaaaaa caggaaggca aaatgccgca aaaaagggaa taagggcgac
     5941 acggaaatgt tgaatactca tactcttcct ttttcaatca tggccgcggg attaaaagtc
     6001 ggggattggt gaacaaaaag gtgtttctct ctttaagaga aatatcgttt tgctaaacag
     6061 ttgatattga ggtatcattt tatcgtaaaa gacatttttg ctcaacaatt gcttgacgga
     6121 aatcaacaaa ttttagcatt ttgtaaaaaa gtcgctatat aatttggtga attggagtta
     6181 ttttcatatt tttgcatccc gaagagtttc tcttaaagag agaaacatct tttgcatacc
     6241 ttttccgacc gaatttttat gtcgtaaaga ggggctttgc agggggtgga ctcagaaaga
     6301 tgagaataga tgactattgt agttgaaaca catagaaagt tgctgatata cagaccgata
     6361 cgcatatcgg gatgaaccat gagtacgttc ttttctcaaa aaacataaat attcgaaaag
     6421 agatgcaata aattaaggag aggttataat gaacaaagta aatataaaag atagtcaaaa
     6481 ttttattact tcaaaatatc acatagaaaa aataatgaat tgcataagtt tagatgaaaa
     6541 agataacatc tttgaaatag gtgcagggaa aggtcatttt actgctggat tggtaaagag
     6601 atgtaatttt gtaacggcga tagaaattga ttctaaatta tgtgaggtaa ctcgtaataa
     6661 gctcttaaat tatcctaact atcaaatagt aaatgatgat atactgaaat ttacatttcc
     6721 tagccacaat ccatataaaa tatttggcaa cataccttac aacataagca caaatataat
     6781 tcgaaaaatt gtttttgaaa gttcagccac aataagttat ttaatagtgg aatatggttt
     6841 tgctaaaatg ttattagata caaacagatc actagcattg ctgttaatgg cagaggtaga
     6901 tatttctata ttagcaaaaa ttcctaggta ttatttccat ccaaaaccta aagtggatag
     6961 cacattaatt gtattaaaaa gaaagccagc aaaaatggca tttaaagaga gaaaaaaata
     7021 tgaaactttt gtaatgaaat gggttaacaa agagtacgaa aaactgttta caaaaaatca
     7081 atttaataaa gctttaaaac atgcgagaat atatgatata aacaatatta gtttcgaaca
     7141 atttgtatcg ctatttaata gttataaaat atttaacggc taaaaacaat aggccacatg
     7201 caactgtaaa tgtttacgcg ggtaccgaca ccgcggtgga ggggaattcc catgtcagcc
     7261 gttaagtgtt cctgtgtcac tcaaaattgc tttgagaggc tctaagggct tctcagtgcg
     7321 ttacatccct ggcttgttgt ccacaaccgt taaaccttaa aagctttaaa agccttatat
     7381 attctttttt ttcttataaa acttaaaacc ttagaggcta tttaagttgc tgatttatat
     7441 taattttatt gttcaaacat gagagcttag tacgtgaaac atgagagctt agtacgttag
     7501 ccatgagagc ttagtacgtt agccatgagg gtttagttcg ttaaacatga gagcttagta
     7561 cgttaaacat gagagcttag tacgtgaaac atgagagctt agtacgtact atcaacaggt
     7621 tgaactgctg atcttcagat cctctacgcc ggacgcatcg tggccggatc aattccgttt
     7681 tccgctgcat aaccctgctt cggggtcatt atagcgattt tttcggtata tccatccttt
     7741 ttcgcacgat atacaggatt ttgccaaagg gttcgtgtag actttccttg gtgtatccaa
     7801 cggcgtcagc cgggcaggat aggtgaagta ggcccacccg cgagcgggtg ttccttcttc
     7861 actgtccctt attcgcacct ggcggtgctc aacgggaatc ctgctctgcg aggctggccg
     7921 gctaccgccg gcgtaacaga tgagggcaag cggatggctg atgaaaccaa gccaaccagg
     7981 aagggcagcc cacctatcac ggaattgatc cccctcgaat tggaattcta gcctggaata
     8041 gggaagcacc acatcacc
//
