LOCUS       sequence-496760-        8113 bp    DNA     circular SYN 09-JAN-2026
DEFINITION  PBT1318 biosensor circuit in pNBU2 integrative Bacteroides plasmid.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8113)
  TITLE     Tropini Lab plasmids
REFERENCE   2  (bases 1 to 8113)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Jan 9, 2026 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pAH109
FEATURES             Location/Qualifiers
     source          1..8113
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             207..893
                     /codon_start=1
                     /product="bright cysteine-free monomeric red fluorescent 
                     protein with fast and complete maturation (Gadella et al., 
                     2023)"
                     /label=mScarlet3
                     /translation="MDSTEAVIKEFMRFKVHMEGSMNGHEFEIEGEGEGRPYEGTQTAK
                     LKVTKGGPLPFSWDILSPQFMYGSRAFIKHPADIPDYWKQSFPEGFKWERVMIFEDGGT
                     VSVTQDTSLEDGTLIYKVKLRGGNFPPDGPVMQKRTMGWEASTERLYPEDVVLKGDIKM
                     ALRLKDGGRYLADFKTTYKAKKPVQMPGAFNIDRKLDITSHNEDYTVVEQYERSVARHS
                     TGGSGGS"
     primer_bind     complement(337..355)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     terminator      919..946
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     CDS             1516..2139
                     /codon_start=1
                     /gene="tetR from transposon Tn10"
                     /product="tetracycline repressor TetR"
                     /label=TetR
                     /note="TetR binds to the tetracycline operator tetO to 
                     inhibit transcription. This inhibition can be relieved by 
                     adding tetracycline or doxycycline."
                     /translation="MSRLDKSKVINSALELLNEVGIEGLTTRKLAQKLGVEQPTLYWHV
                     KNKRALLDALAIEMLDRHHTHFCPLEGESWQDFLRNNAKSFRCALLSHRDGAKVHLGTR
                     PTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEDQEHQVAKEERETPTT
                     DSMPPLLRQAIELFDHQGAEPAFLFGLELIICGLEKQLKCESGS"
     primer_bind     complement(1589..1608)
                     /label=Tet-R
                     /note="Tetracycline resistance gene, reverse primer"
     protein_bind    2304..2322
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     CDS             join(2409..2411,2412..2414,2415..3128)
                     /codon_start=1
                     /product="monomeric GFP derivative that displays 
                     exceptionally high brightness in cells (Campbell et al., 
                     2020)"
                     /label=mGreenLantern
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVRGEGEGDATNGKLTL
                     KFICTTGKLPVPWPTLVTTLGYGVACFARYPDHMKQHDFFKSAMPEGYVQERTISFKDD
                     GTYKTRAEVKFEGDTLVNRIVLKGIDFKEDGNILGHKLEYNFNSHKVYITADKQKNGIK
                     ANFKTRHNVEDGGVQLADHYQQNTPIGDGPVLLPDNHYLSHQSKLSKDPNEKRDHMVLK
                     ERVTAAGITHDMDELYK"
     primer_bind     complement(2454..2475)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     terminator      3149..3176
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     CDS             complement(join(5156..5947,5948..6016))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     5779..5798
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      7308..7696
                     /label=R6K gamma ori
                     /note="gamma replication origin from E. coli plasmid R6K; 
                     requires the R6K initiator protein pi for replication"
     oriT            7867..7976
                     /note="incP origin of transfer"
ORIGIN
        1 caattgggct accttttttt tgttttgttt gcaatggtta atctattgtt aaaatttaaa
       61 gtttcacttg aactttcaaa taatgttctt atatttgcag tgtcgaaaga aacaaagtag
      121 gactgatcac acaacattta aaaaataaca ttatgaaagc actcgaaaag agattcaaag
      181 gtaatattaa caataattta ttttcaatgg atagcaccga ggcagtgatc aaggagttca
      241 tgcggttcaa ggtgcacatg gagggctcca tgaacggcca cgagttcgag atcgagggcg
      301 agggcgaggg ccgcccctac gagggcaccc agaccgccaa gctgaaggtg accaagggtg
      361 gccccctgcc cttctcctgg gacatcctgt cccctcagtt catgtacggc tccagggcct
      421 tcatcaagca ccccgccgac atccccgact actggaagca gtccttcccc gagggcttca
      481 agtgggagcg cgtgatgatc ttcgaggacg gcggcaccgt gtccgtgacc caggacacct
      541 ccctggagga cggcaccctg atctacaagg tgaagctccg cggcggcaac ttccctcctg
      601 acggccccgt aatgcagaag aggacaatgg gctgggaagc atccaccgag cggttgtacc
      661 ccgaggacgt cgtgctgaag ggcgacatta agatggccct gcgcctgaag gacggcggcc
      721 gctacctggc ggacttcaag accacctaca aggccaagaa gcccgtgcag atgcccggcg
      781 ccttcaacat cgaccgcaag ttggacatca cctcccacaa cgaggactac accgtggtgg
      841 aacagtacga acgctccgtg gcccgccact ccaccggcgg ctccggtggc tcctaataaa
      901 tcctagctga ttagctcgag aaggccatcc tgacggatgg cctttttttt gtatggggac
      961 tctgagaacc ctacctttca aaccccgacc ttatcgccca ctttcagcaa accaagattc
     1021 gaacggtcca acgtttcttt catggcagtt accgtatacg tatcatcggt caagttcact
     1081 accgtcaggc atactccatt gtgagaaata ctctgatcaa tcttcagctc atttacaaac
     1141 gaacacttaa atgtaaagtg tatattctcc tggtctttca ccagtgctac caaggtagca
     1201 tactcttcta cgattccaga aaacataatg ttaattatta atgttctaca ataaaagact
     1261 gactaaaaag ggaaataaga gagcttctaa accttttagc caactatact aatatattag
     1321 ctttttcatc aacagctgcg ctaatataat agcacaaaga tacgaataag gaaggaagtt
     1381 ggaaaacttt ctgttttttt tcatatattg caaaggttaa cttgtgaata aattccgccc
     1441 gttcgttcat tttatttttt aaagagaaaa aataaacgga gttttgtgat aggttttact
     1501 aaacaagaag aatatatgtc cagattagat aaaagtaaag tgattaacag cgcattagag
     1561 ctgcttaatg aggtcggaat cgaaggttta acaacccgta aactcgccca gaagctaggt
     1621 gtagagcagc ctacattgta ttggcatgta aaaaataagc gggctttgct cgacgcctta
     1681 gccattgaga tgttagatag gcaccatact cacttttgcc ctttagaagg ggaaagctgg
     1741 caagattttt tacgtaataa cgctaaaagt tttagatgtg ctttactaag tcatcgcgat
     1801 ggagcaaaag tacatttagg tacacggcct acagaaaaac agtatgaaac tctcgaaaat
     1861 caattagcct ttttatgcca acaaggtttt tcactagaga atgcattata tgcactcagc
     1921 gctgtggggc attttacttt aggttgcgta ttggaagatc aagagcatca agtcgctaaa
     1981 gaagaaaggg aaacacctac tactgatagt atgccgccat tattacgaca agctatcgaa
     2041 ttatttgatc accaaggtgc agagccagcc ttcttattcg gccttgaatt gatcatatgc
     2101 ggattagaaa aacaacttaa atgtgaaagt gggtcttaat ggccacatcg gttcctgcta
     2161 gaaggcccat cctgacggat ggccttttgg tattcgagca ttacctcata ctttggattt
     2221 ttgttttgtt tgcaatggtt aatctattgt taaaatttaa agtttcactt gaactttcaa
     2281 ataatgttct tatatttgca gtgtccctat cagtgataga gagtaggatc acacaacatt
     2341 taaaaaataa cattatgaaa gcactcgaaa agagattcaa aggtaatatt aacaataatt
     2401 tattttcaat ggtgagcaag ggcgaggagc tgttcaccgg ggtggtgccc atcctggtcg
     2461 agctggacgg cgacgtaaac ggccacaagt tcagcgtccg cggcgagggc gagggcgatg
     2521 ccaccaacgg caagctgacc ctgaagttca tctgcaccac cggcaagctg cccgtgccct
     2581 ggcccaccct cgtgaccacc ttaggctacg gcgtggcctg cttcgcccgc taccccgacc
     2641 acatgaagca gcacgacttc ttcaagtccg ccatgcccga aggctacgtc caggagcgca
     2701 ccatctcttt caaggacgac ggtacctaca agacccgcgc cgaggtgaag ttcgagggcg
     2761 acaccctggt gaaccgcatc gtgctgaagg gcatcgactt caaggaggac ggcaacatcc
     2821 tggggcacaa gctggagtac aacttcaaca gccacaaggt ctatatcacg gccgacaagc
     2881 agaagaacgg catcaaggct aacttcaaga cccgccacaa cgttgaggac ggcggcgtgc
     2941 agctcgccga ccactaccag cagaacaccc ccatcggcga cggccccgtg ctgctgcccg
     3001 acaaccacta cctgagccat cagtccaagc tgagcaaaga ccccaacgag aagcgcgatc
     3061 acatggtcct gaaggagagg gtgaccgccg ccgggattac acatgacatg gacgagctgt
     3121 acaagtaata gcgttcagct cgactgtgag aaggccatcc tgacggatgg cctttttttt
     3181 tcgttataac tagttgcgtt cctagccgct actagtgtaa ttgcctatct tccagtgatg
     3241 gaacagcatt tgtgcattgg ctgcaacaat cagccttact tgtgcctgtt ctatttccga
     3301 accgaccgct tgtatgaatg catcaaaatt cgttttctct acgttggatt ccttgttgct
     3361 catattgtga tgataatttc tacaaatata gtcattggta actatctatg aaactgtttg
     3421 atacttttat agttgattaa acttgttcat ggcatttgcc ttaatatcat ccgctatgtc
     3481 aatgtagggt ttcatagctt tgtagtcgct gtgtcccgtc catttcatga ccacctgtgc
     3541 cgggattccg agagccagcg cattgcagat gaatgtcctt tttcctgcat gggtactgag
     3601 caaagcgtat ttgtgtgtga cttcatcaat acgttcattt cccttgtagt aggtttcccg
     3661 tacaggctcg ttgatttctg ccagttcgcc cagctctttc aggtaatcgt tcatcttctg
     3721 gttgctgatg acgggcagag ccatgtaatt ctcgaaatgg atgtccttgt atttgtccag
     3781 tatggctttg ctgtatttgt tcagttcaat cgtcaggctg tcggcagtct tgactgtggt
     3841 tatttcgatg tggtcggact tcacatcgct tcttttcaga ttgcgaacat ccgaataccg
     3901 caaactcgta aagcagcaga acaggaaaac atcacgcaca cgttccaggt attgcttatc
     3961 cttgggtatc tggtagtctt tcagcttgtt cagttcatcc caagtcagga agattacttt
     4021 tttcgaggtg gttttcagtt tcggtttgaa cgtatcgtat gcaatgttct gatgatgtcc
     4081 tttcttgaag ctccagcgca ggaaccattt gaggaatccc atttgcttgc cgatggtgct
     4141 gtttctcata tccttggtgt cacgcaggaa gttgacgtat tcgttcaatc caaactcgtt
     4201 gaaatagttg aacgttgcat cctccttgaa ctctttgagg tggttcctca ctgctgcaaa
     4261 tttttcatag gtggatgccg tccagttatt ctggttaccg cactctttta caaactcatc
     4321 gaacacctcc caaaagctga caggggcttc ttccggctgt tcttcactgg tgtctttcat
     4381 tctcatgttg aaagcttcct tcaactgttg ggtcgttggc atgacctcct gcacctcaaa
     4441 ttccttgaaa atattctgga tttcggcata gtatttcagc aagtccgtat tgatttcggc
     4501 tgcactttgc tttagcttgt tggtacatcc gttctttacc cgctgcttat ctgcatccca
     4561 tttggctacg tcaatccggt agcccgttgt aaactcgata cgttggctgg caaagatgac
     4621 acgcatacgg atgggtacgt tctctacgat tggcacaccg ttctttttcc ggctctccaa
     4681 tgcaaaaatg atgttgcgct tgatattcat aattgggtgc gtttgaaatt ctacacccaa
     4741 atatacaccc aattattgag atagcaaaag acatttagaa acatttactt ttactctata
     4801 ttgtaattta cacttgatta tcagtcgttt gcagttttat gatattctgt gaaagtataa
     4861 gttcgagagc ctgtctctcc gcaaaaaacg ctgaaaatca gcagattgca aaacaaacac
     4921 cctgttttac acccaagaat gtaaagtcgg gtgtttttgt tttatttaag ataatacaac
     4981 cactacataa taaaagagta gcgatattaa aagaatccga tgagaaaaga ctaatattta
     5041 tctatccatt cagtttgatt tctcaggact ttacatcgtc ctgaaagtat ttgttggtac
     5101 cggtaccgag gacgcgacag ctgcagacgc gttatgagta aacttggtct gacagttacc
     5161 aatgcttaat cagtgaggca cctatctcag cgatctgtct atttcgttca tccatagttg
     5221 cctgactccc cgtcgtgtag ataactacga tacgggaggg cttaccatct ggccccagtg
     5281 ctgcaatgat accgcgggac ccacgctcac cggctccaga tttatcagca ataaaccagc
     5341 cagccggaag ggccgagcgc agaagtggtc ctgcaacttt atccgcctcc atccagtcta
     5401 ttaattgttg ccgggaagct agagtaagta gttcgccagt taatagtttg cgcaacgttg
     5461 ttgccattgc tacaggcatc gtggtgtcac gctcgtcgtt tggtatggct tcattcagct
     5521 ccggttccca acgatcaagg cgagttacat gatcccccat gttgtgcaaa aaagcggtta
     5581 gctccttcgg tcctccgatc gttgtcagaa gtaagttggc cgcagtgtta tcactcatgg
     5641 ttatggcagc actgcataat tctcttactg tcatgccatc cgtaagatgc ttttctgtga
     5701 ctggtgagta ctcaaccaag tcattctgag aatagtgtat gcggcgaccg agttgctctt
     5761 gcccggcgtc aatacgggat aataccgcgc cacatagcag aactttaaaa gtgctcatca
     5821 ttggaaaacg ttcttcgggg cgaaaactct caaggatctt accgctgttg agatccagtt
     5881 cgatgtaacc cactcgtgca cccaactgat cttcagcatc ttttactttc accagcgttt
     5941 ctgggtgagc aaaaacagga aggcaaaatg ccgcaaaaaa gggaataagg gcgacacgga
     6001 aatgttgaat actcatactc ttcctttttc aatcatggcc gcgggattaa aagtcgggga
     6061 ttggtgaaca aaaaggtgtt tctctcttta agagaaatat cgttttgcta aacagttgat
     6121 attgaggtat cattttatcg taaaagacat ttttgctcaa caattgcttg acggaaatca
     6181 acaaatttta gcattttgta aaaaagtcgc tatataattt ggtgaattgg agttattttc
     6241 atatttttgc atcccgaaga gtttctctta aagagagaaa catcttttgc ataccttttc
     6301 cgaccgaatt tttatgtcgt aaagaggggc tttgcagggg gtggactcag aaagatgaga
     6361 atagatgact attgtagttg aaacacatag aaagttgctg atatacagac cgatacgcat
     6421 atcgggatga accatgagta cgttcttttc tcaaaaaaca taaatattcg aaaagagatg
     6481 caataaatta aggagaggtt ataatgaaca aagtaaatat aaaagatagt caaaatttta
     6541 ttacttcaaa atatcacata gaaaaaataa tgaattgcat aagtttagat gaaaaagata
     6601 acatctttga aataggtgca gggaaaggtc attttactgc tggattggta aagagatgta
     6661 attttgtaac ggcgatagaa attgattcta aattatgtga ggtaactcgt aataagctct
     6721 taaattatcc taactatcaa atagtaaatg atgatatact gaaatttaca tttcctagcc
     6781 acaatccata taaaatattt ggcaacatac cttacaacat aagcacaaat ataattcgaa
     6841 aaattgtttt tgaaagttca gccacaataa gttatttaat agtggaatat ggttttgcta
     6901 aaatgttatt agatacaaac agatcactag cattgctgtt aatggcagag gtagatattt
     6961 ctatattagc aaaaattcct aggtattatt tccatccaaa acctaaagtg gatagcacat
     7021 taattgtatt aaaaagaaag ccagcaaaaa tggcatttaa agagagaaaa aaatatgaaa
     7081 cttttgtaat gaaatgggtt aacaaagagt acgaaaaact gtttacaaaa aatcaattta
     7141 ataaagcttt aaaacatgcg agaatatatg atataaacaa tattagtttc gaacaatttg
     7201 tatcgctatt taatagttat aaaatattta acggctaaaa acaataggcc acatgcaact
     7261 gtaaatgttt acgcgggtac cgacaccgcg gtggagggga attcccatgt cagccgttaa
     7321 gtgttcctgt gtcactcaaa attgctttga gaggctctaa gggcttctca gtgcgttaca
     7381 tccctggctt gttgtccaca accgttaaac cttaaaagct ttaaaagcct tatatattct
     7441 tttttttctt ataaaactta aaaccttaga ggctatttaa gttgctgatt tatattaatt
     7501 ttattgttca aacatgagag cttagtacgt gaaacatgag agcttagtac gttagccatg
     7561 agagcttagt acgttagcca tgagggttta gttcgttaaa catgagagct tagtacgtta
     7621 aacatgagag cttagtacgt gaaacatgag agcttagtac gtactatcaa caggttgaac
     7681 tgctgatctt cagatcctct acgccggacg catcgtggcc ggatcaattc cgttttccgc
     7741 tgcataaccc tgcttcgggg tcattatagc gattttttcg gtatatccat cctttttcgc
     7801 acgatataca ggattttgcc aaagggttcg tgtagacttt ccttggtgta tccaacggcg
     7861 tcagccgggc aggataggtg aagtaggccc acccgcgagc gggtgttcct tcttcactgt
     7921 cccttattcg cacctggcgg tgctcaacgg gaatcctgct ctgcgaggct ggccggctac
     7981 cgccggcgta acagatgagg gcaagcggat ggctgatgaa accaagccaa ccaggaaggg
     8041 cagcccacct atcacggaat tgatccccct cgaattggaa ttctagcctg gaatagggaa
     8101 gcaccacatc acc
//
