LOCUS       sequence-496763-        8083 bp    DNA     circular SYN 09-JAN-2026
DEFINITION  PBT0981 biosensor circuit in pNBU2 integrative Bacteroides plasmid.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8083)
  TITLE     Tropini Lab plasmids
REFERENCE   2  (bases 1 to 8083)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Jan 9, 2026 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pAH121
FEATURES             Location/Qualifiers
     source          1..8083
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             207..893
                     /codon_start=1
                     /product="bright cysteine-free monomeric red fluorescent 
                     protein with fast and complete maturation (Gadella et al., 
                     2023)"
                     /label=mScarlet3
                     /translation="MDSTEAVIKEFMRFKVHMEGSMNGHEFEIEGEGEGRPYEGTQTAK
                     LKVTKGGPLPFSWDILSPQFMYGSRAFIKHPADIPDYWKQSFPEGFKWERVMIFEDGGT
                     VSVTQDTSLEDGTLIYKVKLRGGNFPPDGPVMQKRTMGWEASTERLYPEDVVLKGDIKM
                     ALRLKDGGRYLADFKTTYKAKKPVQMPGAFNIDRKLDITSHNEDYTVVEQYERSVARHS
                     TGGSGGS"
     primer_bind     complement(337..355)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     terminator      919..946
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     CDS             1486..2109
                     /codon_start=1
                     /gene="tetR from transposon Tn10"
                     /product="tetracycline repressor TetR"
                     /label=TetR
                     /note="TetR binds to the tetracycline operator tetO to 
                     inhibit transcription. This inhibition can be relieved by 
                     adding tetracycline or doxycycline."
                     /translation="MSRLDKSKVINSALELLNEVGIEGLTTRKLAQKLGVEQPTLYWHV
                     KNKRALLDALAIEMLDRHHTHFCPLEGESWQDFLRNNAKSFRCALLSHRDGAKVHLGTR
                     PTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEDQEHQVAKEERETPTT
                     DSMPPLLRQAIELFDHQGAEPAFLFGLELIICGLEKQLKCESGS"
     primer_bind     complement(1559..1578)
                     /label=Tet-R
                     /note="Tetracycline resistance gene, reverse primer"
     protein_bind    2274..2292
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     CDS             join(2379..2381,2382..2384,2385..3098)
                     /codon_start=1
                     /product="monomeric GFP derivative that displays 
                     exceptionally high brightness in cells (Campbell et al., 
                     2020)"
                     /label=mGreenLantern
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVRGEGEGDATNGKLTL
                     KFICTTGKLPVPWPTLVTTLGYGVACFARYPDHMKQHDFFKSAMPEGYVQERTISFKDD
                     GTYKTRAEVKFEGDTLVNRIVLKGIDFKEDGNILGHKLEYNFNSHKVYITADKQKNGIK
                     ANFKTRHNVEDGGVQLADHYQQNTPIGDGPVLLPDNHYLSHQSKLSKDPNEKRDHMVLK
                     ERVTAAGITHDMDELYK"
     primer_bind     complement(2424..2445)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     terminator      3119..3146
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     CDS             complement(join(5126..5917,5918..5986))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     5749..5768
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      7278..7666
                     /label=R6K gamma ori
                     /note="gamma replication origin from E. coli plasmid R6K; 
                     requires the R6K initiator protein pi for replication"
     oriT            7837..7946
                     /note="incP origin of transfer"
ORIGIN
        1 caattgggct accttttttt tgttttgttt gcaatggtta atctattgtt aaaatttaaa
       61 gtttcacttg aactttcaaa taatgttctt atatttgcag tgtcgaaaga aacaaagtag
      121 gactgatcac acaacattta aaaaataaca ttatgaaagc actcgaaaag agattcaaag
      181 gtaatattaa caataattta ttttcaatgg atagcaccga ggcagtgatc aaggagttca
      241 tgcggttcaa ggtgcacatg gagggctcca tgaacggcca cgagttcgag atcgagggcg
      301 agggcgaggg ccgcccctac gagggcaccc agaccgccaa gctgaaggtg accaagggtg
      361 gccccctgcc cttctcctgg gacatcctgt cccctcagtt catgtacggc tccagggcct
      421 tcatcaagca ccccgccgac atccccgact actggaagca gtccttcccc gagggcttca
      481 agtgggagcg cgtgatgatc ttcgaggacg gcggcaccgt gtccgtgacc caggacacct
      541 ccctggagga cggcaccctg atctacaagg tgaagctccg cggcggcaac ttccctcctg
      601 acggccccgt aatgcagaag aggacaatgg gctgggaagc atccaccgag cggttgtacc
      661 ccgaggacgt cgtgctgaag ggcgacatta agatggccct gcgcctgaag gacggcggcc
      721 gctacctggc ggacttcaag accacctaca aggccaagaa gcccgtgcag atgcccggcg
      781 ccttcaacat cgaccgcaag ttggacatca cctcccacaa cgaggactac accgtggtgg
      841 aacagtacga acgctccgtg gcccgccact ccaccggcgg ctccggtggc tcctaataaa
      901 tcctagctga ttagctcgag aaggccatcc tgacggatgg cctttttttt gtatggggac
      961 tctgagaacc ctacctttca aaccccgaag ttctcacgga aagggaataa tggtgatata
     1021 tatatacata aaccttaaac aggctctgtt tttatatcct atcatgaaat atttcccgaa
     1081 catagtggga accggtagca agaatcgtgt ttctaaaaaa gcaaataggc gttacaaagc
     1141 ttatagatgt gtttttatag gtttatttac cccttttgtt ctttttgaaa agcctaatac
     1201 tggattttta gactttactg gaatttgaga atgaaaaatg aggtagttta gatcgaaata
     1261 cagcatttaa tgtaagtact taaatatgac attcgctttt gttgaatctt tgcttcggat
     1321 cattgctttg gggctgtttc cagttcagct ttagcactga aattctcgcg aaatttcaat
     1381 gaatatcttc gatgataaaa aagataagtt ataaaattca gtttcttttt taaagataaa
     1441 atagtatctt tgatgcaaga ttcatttcca ctaatataat atacaatgtc cagattagat
     1501 aaaagtaaag tgattaacag cgcattagag ctgcttaatg aggtcggaat cgaaggttta
     1561 acaacccgta aactcgccca gaagctaggt gtagagcagc ctacattgta ttggcatgta
     1621 aaaaataagc gggctttgct cgacgcctta gccattgaga tgttagatag gcaccatact
     1681 cacttttgcc ctttagaagg ggaaagctgg caagattttt tacgtaataa cgctaaaagt
     1741 tttagatgtg ctttactaag tcatcgcgat ggagcaaaag tacatttagg tacacggcct
     1801 acagaaaaac agtatgaaac tctcgaaaat caattagcct ttttatgcca acaaggtttt
     1861 tcactagaga atgcattata tgcactcagc gctgtggggc attttacttt aggttgcgta
     1921 ttggaagatc aagagcatca agtcgctaaa gaagaaaggg aaacacctac tactgatagt
     1981 atgccgccat tattacgaca agctatcgaa ttatttgatc accaaggtgc agagccagcc
     2041 ttcttattcg gccttgaatt gatcatatgc ggattagaaa aacaacttaa atgtgaaagt
     2101 gggtcttaat ggccacatcg gttcctgcta gaaggcccat cctgacggat ggccttttgg
     2161 tattcgagca ttacctcata ctttggattt ttgttttgtt tgcaatggtt aatctattgt
     2221 taaaatttaa agtttcactt gaactttcaa ataatgttct tatatttgca gtgtccctat
     2281 cagtgataga gagtaggatc acacaacatt taaaaaataa cattatgaaa gcactcgaaa
     2341 agagattcaa aggtaatatt aacaataatt tattttcaat ggtgagcaag ggcgaggagc
     2401 tgttcaccgg ggtggtgccc atcctggtcg agctggacgg cgacgtaaac ggccacaagt
     2461 tcagcgtccg cggcgagggc gagggcgatg ccaccaacgg caagctgacc ctgaagttca
     2521 tctgcaccac cggcaagctg cccgtgccct ggcccaccct cgtgaccacc ttaggctacg
     2581 gcgtggcctg cttcgcccgc taccccgacc acatgaagca gcacgacttc ttcaagtccg
     2641 ccatgcccga aggctacgtc caggagcgca ccatctcttt caaggacgac ggtacctaca
     2701 agacccgcgc cgaggtgaag ttcgagggcg acaccctggt gaaccgcatc gtgctgaagg
     2761 gcatcgactt caaggaggac ggcaacatcc tggggcacaa gctggagtac aacttcaaca
     2821 gccacaaggt ctatatcacg gccgacaagc agaagaacgg catcaaggct aacttcaaga
     2881 cccgccacaa cgttgaggac ggcggcgtgc agctcgccga ccactaccag cagaacaccc
     2941 ccatcggcga cggccccgtg ctgctgcccg acaaccacta cctgagccat cagtccaagc
     3001 tgagcaaaga ccccaacgag aagcgcgatc acatggtcct gaaggagagg gtgaccgccg
     3061 ccgggattac acatgacatg gacgagctgt acaagtaata gcgttcagct cgactgtgag
     3121 aaggccatcc tgacggatgg cctttttttt tcgttataac tagttgcgtt cctagccgct
     3181 actagtgtaa ttgcctatct tccagtgatg gaacagcatt tgtgcattgg ctgcaacaat
     3241 cagccttact tgtgcctgtt ctatttccga accgaccgct tgtatgaatg catcaaaatt
     3301 cgttttctct acgttggatt ccttgttgct catattgtga tgataatttc tacaaatata
     3361 gtcattggta actatctatg aaactgtttg atacttttat agttgattaa acttgttcat
     3421 ggcatttgcc ttaatatcat ccgctatgtc aatgtagggt ttcatagctt tgtagtcgct
     3481 gtgtcccgtc catttcatga ccacctgtgc cgggattccg agagccagcg cattgcagat
     3541 gaatgtcctt tttcctgcat gggtactgag caaagcgtat ttgtgtgtga cttcatcaat
     3601 acgttcattt cccttgtagt aggtttcccg tacaggctcg ttgatttctg ccagttcgcc
     3661 cagctctttc aggtaatcgt tcatcttctg gttgctgatg acgggcagag ccatgtaatt
     3721 ctcgaaatgg atgtccttgt atttgtccag tatggctttg ctgtatttgt tcagttcaat
     3781 cgtcaggctg tcggcagtct tgactgtggt tatttcgatg tggtcggact tcacatcgct
     3841 tcttttcaga ttgcgaacat ccgaataccg caaactcgta aagcagcaga acaggaaaac
     3901 atcacgcaca cgttccaggt attgcttatc cttgggtatc tggtagtctt tcagcttgtt
     3961 cagttcatcc caagtcagga agattacttt tttcgaggtg gttttcagtt tcggtttgaa
     4021 cgtatcgtat gcaatgttct gatgatgtcc tttcttgaag ctccagcgca ggaaccattt
     4081 gaggaatccc atttgcttgc cgatggtgct gtttctcata tccttggtgt cacgcaggaa
     4141 gttgacgtat tcgttcaatc caaactcgtt gaaatagttg aacgttgcat cctccttgaa
     4201 ctctttgagg tggttcctca ctgctgcaaa tttttcatag gtggatgccg tccagttatt
     4261 ctggttaccg cactctttta caaactcatc gaacacctcc caaaagctga caggggcttc
     4321 ttccggctgt tcttcactgg tgtctttcat tctcatgttg aaagcttcct tcaactgttg
     4381 ggtcgttggc atgacctcct gcacctcaaa ttccttgaaa atattctgga tttcggcata
     4441 gtatttcagc aagtccgtat tgatttcggc tgcactttgc tttagcttgt tggtacatcc
     4501 gttctttacc cgctgcttat ctgcatccca tttggctacg tcaatccggt agcccgttgt
     4561 aaactcgata cgttggctgg caaagatgac acgcatacgg atgggtacgt tctctacgat
     4621 tggcacaccg ttctttttcc ggctctccaa tgcaaaaatg atgttgcgct tgatattcat
     4681 aattgggtgc gtttgaaatt ctacacccaa atatacaccc aattattgag atagcaaaag
     4741 acatttagaa acatttactt ttactctata ttgtaattta cacttgatta tcagtcgttt
     4801 gcagttttat gatattctgt gaaagtataa gttcgagagc ctgtctctcc gcaaaaaacg
     4861 ctgaaaatca gcagattgca aaacaaacac cctgttttac acccaagaat gtaaagtcgg
     4921 gtgtttttgt tttatttaag ataatacaac cactacataa taaaagagta gcgatattaa
     4981 aagaatccga tgagaaaaga ctaatattta tctatccatt cagtttgatt tctcaggact
     5041 ttacatcgtc ctgaaagtat ttgttggtac cggtaccgag gacgcgacag ctgcagacgc
     5101 gttatgagta aacttggtct gacagttacc aatgcttaat cagtgaggca cctatctcag
     5161 cgatctgtct atttcgttca tccatagttg cctgactccc cgtcgtgtag ataactacga
     5221 tacgggaggg cttaccatct ggccccagtg ctgcaatgat accgcgggac ccacgctcac
     5281 cggctccaga tttatcagca ataaaccagc cagccggaag ggccgagcgc agaagtggtc
     5341 ctgcaacttt atccgcctcc atccagtcta ttaattgttg ccgggaagct agagtaagta
     5401 gttcgccagt taatagtttg cgcaacgttg ttgccattgc tacaggcatc gtggtgtcac
     5461 gctcgtcgtt tggtatggct tcattcagct ccggttccca acgatcaagg cgagttacat
     5521 gatcccccat gttgtgcaaa aaagcggtta gctccttcgg tcctccgatc gttgtcagaa
     5581 gtaagttggc cgcagtgtta tcactcatgg ttatggcagc actgcataat tctcttactg
     5641 tcatgccatc cgtaagatgc ttttctgtga ctggtgagta ctcaaccaag tcattctgag
     5701 aatagtgtat gcggcgaccg agttgctctt gcccggcgtc aatacgggat aataccgcgc
     5761 cacatagcag aactttaaaa gtgctcatca ttggaaaacg ttcttcgggg cgaaaactct
     5821 caaggatctt accgctgttg agatccagtt cgatgtaacc cactcgtgca cccaactgat
     5881 cttcagcatc ttttactttc accagcgttt ctgggtgagc aaaaacagga aggcaaaatg
     5941 ccgcaaaaaa gggaataagg gcgacacgga aatgttgaat actcatactc ttcctttttc
     6001 aatcatggcc gcgggattaa aagtcgggga ttggtgaaca aaaaggtgtt tctctcttta
     6061 agagaaatat cgttttgcta aacagttgat attgaggtat cattttatcg taaaagacat
     6121 ttttgctcaa caattgcttg acggaaatca acaaatttta gcattttgta aaaaagtcgc
     6181 tatataattt ggtgaattgg agttattttc atatttttgc atcccgaaga gtttctctta
     6241 aagagagaaa catcttttgc ataccttttc cgaccgaatt tttatgtcgt aaagaggggc
     6301 tttgcagggg gtggactcag aaagatgaga atagatgact attgtagttg aaacacatag
     6361 aaagttgctg atatacagac cgatacgcat atcgggatga accatgagta cgttcttttc
     6421 tcaaaaaaca taaatattcg aaaagagatg caataaatta aggagaggtt ataatgaaca
     6481 aagtaaatat aaaagatagt caaaatttta ttacttcaaa atatcacata gaaaaaataa
     6541 tgaattgcat aagtttagat gaaaaagata acatctttga aataggtgca gggaaaggtc
     6601 attttactgc tggattggta aagagatgta attttgtaac ggcgatagaa attgattcta
     6661 aattatgtga ggtaactcgt aataagctct taaattatcc taactatcaa atagtaaatg
     6721 atgatatact gaaatttaca tttcctagcc acaatccata taaaatattt ggcaacatac
     6781 cttacaacat aagcacaaat ataattcgaa aaattgtttt tgaaagttca gccacaataa
     6841 gttatttaat agtggaatat ggttttgcta aaatgttatt agatacaaac agatcactag
     6901 cattgctgtt aatggcagag gtagatattt ctatattagc aaaaattcct aggtattatt
     6961 tccatccaaa acctaaagtg gatagcacat taattgtatt aaaaagaaag ccagcaaaaa
     7021 tggcatttaa agagagaaaa aaatatgaaa cttttgtaat gaaatgggtt aacaaagagt
     7081 acgaaaaact gtttacaaaa aatcaattta ataaagcttt aaaacatgcg agaatatatg
     7141 atataaacaa tattagtttc gaacaatttg tatcgctatt taatagttat aaaatattta
     7201 acggctaaaa acaataggcc acatgcaact gtaaatgttt acgcgggtac cgacaccgcg
     7261 gtggagggga attcccatgt cagccgttaa gtgttcctgt gtcactcaaa attgctttga
     7321 gaggctctaa gggcttctca gtgcgttaca tccctggctt gttgtccaca accgttaaac
     7381 cttaaaagct ttaaaagcct tatatattct tttttttctt ataaaactta aaaccttaga
     7441 ggctatttaa gttgctgatt tatattaatt ttattgttca aacatgagag cttagtacgt
     7501 gaaacatgag agcttagtac gttagccatg agagcttagt acgttagcca tgagggttta
     7561 gttcgttaaa catgagagct tagtacgtta aacatgagag cttagtacgt gaaacatgag
     7621 agcttagtac gtactatcaa caggttgaac tgctgatctt cagatcctct acgccggacg
     7681 catcgtggcc ggatcaattc cgttttccgc tgcataaccc tgcttcgggg tcattatagc
     7741 gattttttcg gtatatccat cctttttcgc acgatataca ggattttgcc aaagggttcg
     7801 tgtagacttt ccttggtgta tccaacggcg tcagccgggc aggataggtg aagtaggccc
     7861 acccgcgagc gggtgttcct tcttcactgt cccttattcg cacctggcgg tgctcaacgg
     7921 gaatcctgct ctgcgaggct ggccggctac cgccggcgta acagatgagg gcaagcggat
     7981 ggctgatgaa accaagccaa ccaggaaggg cagcccacct atcacggaat tgatccccct
     8041 cgaattggaa ttctagcctg gaatagggaa gcaccacatc acc
//
